SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 14526 FitnessBrowser__Keio:14526 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P10880 Shikimate kinase 2; SK 2; Shikimate kinase II; SKII; EC 2.7.1.71 from Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi) (see paper)
54% identity, 94% coverage: 1:164/174 of query aligns to 1:164/173 of P10880

query
sites
P10880

M

M

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T

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E

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P

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C13 (= C13) mutation to S: 66% of wild-type activity. Increase in substrates affinity.
K15 (= K15) mutation to M: Loss of activity. Increased thermostability.
D34 (= D34) mutation to N: Loss of activity.
C162 (≠ S162) mutation to S: No effect on activity and substrates affinity.

1e6cA K15m mutant of shikimate kinase from erwinia chrysanthemi (see paper)
53% identity, 94% coverage: 1:164/174 of query aligns to 1:164/170 of 1e6cA

query
sites
1e6cA

M

M

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M

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R

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M

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binding phosphate ion: R11 (= R11), G12 (= G12), M15 (≠ K15), T16 (= T16)

2shkB The three-dimensional structure of shikimate kinase from erwinia chrysanthemi complexed with adp (see paper)
51% identity, 94% coverage: 1:164/174 of query aligns to 1:154/162 of 2shkB

query
sites
2shkB

M

M

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C

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G

K
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K

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T

V

V

G

G

M

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L

L

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D

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A

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D

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M

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binding adenosine-5'-diphosphate: G12 (= G12), C13 (= C13), G14 (= G14), K15 (= K15), T16 (= T16), T17 (= T17), R110 (= R110), Q145 (≠ N155), P147 (= P157)
binding magnesium ion: Q134 (≠ R144), A137 (= A147)

1shkA The three-dimensional structure of shikimate kinase from erwinia chrysanthemi (see paper)
49% identity, 94% coverage: 1:164/174 of query aligns to 1:149/158 of 1shkA

query
sites
1shkA

M

M

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K

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G

M

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binding magnesium ion: R92 (≠ Q92), A93 (≠ N93), G95 (= G95), Q129 (≠ R144), A132 (= A147)

4y0aA Shikimate kinase from acinetobacter baumannii in complex with shikimate (see paper)
42% identity, 87% coverage: 5:156/174 of query aligns to 11:159/179 of 4y0aA

query
sites
4y0aA

L

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Q

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: M17 (≠ R11), D40 (= D34), F63 (= F57), R64 (= R58), G86 (= G79), G87 (= G80), L126 (≠ T122), R143 (= R139)
binding sulfate ion: G18 (= G12), A19 (≠ C13), G20 (= G14), K21 (= K15), T22 (= T16), E112 (≠ S105), R124 (= R120), R137 (≠ Q133)

3mufA Shikimate kinase from helicobacter pylori in complex with shikimate-3- phosphate and adp (see paper)
32% identity, 93% coverage: 3:164/174 of query aligns to 1:160/160 of 3mufA

query
sites
3mufA

Q

Q

P

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L

L

F

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G

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M

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x
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N

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I

binding adenosine-5'-diphosphate: G10 (= G12), S11 (≠ C13), G12 (= G14), K13 (= K15), S14 (≠ T16), S15 (≠ T17), M98 (≠ A102), R106 (= R110), G147 (vs. gap), G148 (vs. gap)
binding shikimate-3-phosphate: M9 (≠ R11), D32 (= D34), R56 (= R58), G78 (= G78), G79 (= G79), G80 (= G80), R115 (= R120), R131 (= R139)

1zuiA Structural basis for shikimate-binding specificity of helicobacter pylori shikimate kinase (see paper)
32% identity, 93% coverage: 3:164/174 of query aligns to 1:158/158 of 1zuiA

query
sites
1zuiA

Q

Q

P

H

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L

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M

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L

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F

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G

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x
E

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x
R

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L

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N

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N

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G

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K

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L

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N

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I

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: M9 (≠ R11), D32 (= D34), F47 (≠ V49), R56 (= R58), G78 (= G78), G79 (= G79), E111 (≠ P115), R113 (≠ E117), R129 (= R139)

3n2eA Crystal structure of helicobactor pylori shikimate kinase in complex with nsc162535 (see paper)
32% identity, 93% coverage: 3:164/174 of query aligns to 2:161/168 of 3n2eA

query
sites
3n2eA

Q

Q

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L

L

F

V

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L

I

I

G

G

P
x
F

R

M

G

G

C

S

G

G

K

K

T

S

V

L

G

A

M

Q

A

E

L

L

A

G

D

L

S

A

L

L

N

K

R

L

R

E

F

V

V

L

D

D

T

T

D

D

Q

M

W

I

L

I

Q

S

S

E

Q

R

L

V

N

G

M

L

T

S

V

V

A
x
R

E

E

I

I

V
x
F

E

E

R

E

E

L

E

G

W

E

A

D

G

N

F

F

R
|
R

A

M

R

F

E

E

T

K

A

N

A

L

L

I

E

D

A

E

V

L

T

K

A

T

P

L

S

K

T

T

-

P

-

H

V

V

I

I

A

S

T

T

G

G

G
|
G

I

I

I

V

L
x
M

T

H

E

E

F

-

N

-

R

-

H

-

F

N

M

L

Q

K

N

G

N

L

G

G

I

T

V

T

V

F

Y

Y

L

L

C

K

A

M

P

D

V

F

S

E

V

T

L

L

V

I

N

K

R

R

L

L

Q

N

A

-

A

Q

P

K

E

E

E

R

D

A

L

K

R
|
R

P

P

T

L

L

L

T

N

G

N

K

L

P

T

L

-

S

-

E

-

E

Q

V

A

Q

K

E

E

V

L

L

F

E

E

E

K

R
|
R

D

Q

A

A

L
|
L

Y
|
Y

R

E

E

K

V

N

A

A

H

S

I

F

I

I

D

D

A

A

-

R

-

G

-

L

T

N

N

N

E

S

P

L

S

K

Q

Q

V

V

I

L

S

Q

E

F

I

I

binding 7-amino-4-hydroxy-3-[(E)-(5-hydroxy-7-sulfonaphthalen-2-yl)diazenyl]naphthalene-2-sulfonic acid: F9 (≠ P10), R45 (≠ A46), F48 (≠ V49), R57 (= R58), G80 (= G79), G81 (= G80), M84 (≠ L83), R116 (= R120), R132 (= R139), L135 (= L142), Y136 (= Y143)

6hqvA Pentafunctional arom complex from chaetomium thermophilum (see paper)
32% identity, 87% coverage: 5:156/174 of query aligns to 845:996/1555 of 6hqvA

query
sites
6hqvA

L

I

F

F

L

I

I

V

G

G

P

M

R

R

G

G

C

A

G

G

K

K

T

S

T

T

V

A

G

G

M

R

A

W

L

M

A

S

D

E

S

L

L

L

N

K

R

R

R

P

F

L

V

V

D

D

T

L

D
|
D

Q

A

W

E

L

L

Q

E

S

R

Q

R

L

E

N

G

M

M

T

T

V

I

A

P

E

E

I

I

V

I

E

R

R

G

E

E

E

R

-

G

W

W

A

E

G

G

F

F

R

R

A

Q

R

A

E

E

T

L

A

E

A

L

L

L

E

Q

A

D

V

V

T

I

-

K

-

N

-

Q

A

S

P

K

S

G

T

Y

V

I

I

F

A

S

T

C

G

G

G
|
G

I

I

I

V

L

E

T

T

E

E

F

A

N

A

R

R

H

K

F

L

M

L

-

I

-

D

-

Y

Q

H

N

K

N

N

G

G

I

-

V

-

Y

-

L

-

C

-

A

G

P

P

V

V

S

-

V

L

L

L

V

V

N

H

R

R

L

D

Q

T

A

D

A

Q

P

V

E

V

E

E

D

Y

L

L

R

M

P

R

T

D

L

K

T

T

G

R

K

P

P

A

L

Y

S

S

E

E

E

N

V

I

Q

R

E

E

V

V

L

Y

E

E

E

R

R
|
R

D

K

A

P

L

W

Y

F

R

Y

E

E

V

C

A

S

H

N

I

L

I

Q

I

Y

D

H

A

S

T

P

N

H

E

E

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D874 (= D34), G923 (= G79), G924 (= G80), R979 (= R139)

Sites not aligning to the query:

active site: 123, 145, 187, 243, 253, 257, 261, 264, 268, 280
binding (4S,5R)-4,5-dihydroxy-3-oxocyclohex-1-ene-1-carboxylic acid: 1060, 1062, 1181, 1224, 1232, 1242, 1243
binding glutamic acid: 139, 145, 187, 243, 257, 264, 280
binding nicotinamide-adenine-dinucleotide: 42, 44, 45, 76, 79, 107, 108, 109, 112, 132, 133, 135, 139, 140, 145, 154, 175, 176, 177, 180, 280
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 413, 562, 563, 1277, 1279, 1323, 1327, 1348, 1368, 1526
binding zinc ion: 187, 264, 280

P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
28% identity, 83% coverage: 5:149/174 of query aligns to 866:1009/1583 of P07547

query
sites
P07547

L

I

F

Y

L

I

I

I

G

G

P

M

R

R

G

G

C

A

G

G

K

K

T

S

T

T

V

A

G

G

M

N

A

W

L

V

A

S

D

K

S

A

L

L

N

N

R

R

R

P

F

F

V

V

D

D

T

L

D

D

Q

T

W

E

L

L

Q

E

S

T

Q

V

L

E

N

G

M

M

T

T

V

I

A

P

E

D

I

I

V

I

E

K

R

T

E

R

E

G

W

W

A

Q

G

G

F

F

R

R

A

N

R

A

E

E

T

L

A

E

A

I

L

L

E

K

A

R

V

T

T

L

A

K

P

E

S

R

T

S

-

R

-

G

-

Y

V

V

I

F

A

A

T

C

G

G

I

V

-

E

-

M

-

P

-

E

-

A

-

R

-

K

-

L

L

L

T

T

E

D

F

Y

N

H

R

K

H

-

F

-

M

-

Q

-

N

T

N

K

G

G

I

N

V

V

V

L

Y

L

L

L

C

M

A

R

P

D

V

I

S

K

V

K

L

I

V

M

N

D

R

F

L

L

Q

S

A

I

A

-

P

-

E

-

E

D

D

K

L

T

R

R

P

P

T

A

L

Y

T

V

G

-

K

-

P

-

L

-

S

-

E

E

E

D

V

M

Q

M

E

G

V

V

L

W

E

L

E

R

R

R

D

K

A

P

L

W

Y

F

R

Q

E

E

V

C

A

S

H

N

I

I

Sites not aligning to the query:

44:46 binding
81:84 binding
114:116 binding
119 binding
139:140 binding
161 binding
179:182 binding
190 binding
194 binding
271 binding
287 binding

2iywA Shikimate kinase from mycobacterium tuberculosis in complex with mgatp, open lid (conf. B) (see paper)
29% identity, 93% coverage: 7:168/174 of query aligns to 6:165/178 of 2iywA

query
sites
2iywA

L

L

I

V

G

G

P

L

R

P

G
|
G

C

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

M

R

A

R

L

L

A

A

D

K

S

A

L

L

N

G

R

V

R

G

F

L

V

L

D

D

T

T

D

D

Q

V

W

A

L

I

Q

E

S

Q

Q

R

L

T

N

G

M

R

T

S

V

I

A

A

E

D

I

I

V

F

E

A

R

T

E

D

E

G

W

E

A

Q

G

E

F

F

R

R

A

R

R

I

E

E

T

E

A

D

A

V

L

V

E

R

A

A

V

A

T

L

A

A

P

D

-

H

S

D

T

G

V

V

I

L

A

S

T

L

G

G

I

A

I

V

L

T

T

S

E

P

F

G

N

V

R

R

H

A

F

A

M

L

Q

A

N

G

N

H

G

-

I

T

V

V

Y

Y

L

L

C

E

A

I

P

S

V

A

S

A

V

E

L

G

V

V

N

R

R
|
R

L

T

Q

G

A

G

A

-

P

-

E

-

E

N

D

T

L

V

R

R

P

P

T

L

L

L

T

A

G

G

K

P

P

D

L

R

S

A

E

E

E

K

V

Y

Q

R

E

A

V

L

L

M

E

A

E

K

R

R

D

A

A

P

L

L

Y

Y

R

R

E

R

V

V

A

A

H

T

I

M

I

R

I

V

D

D

A

T

T

N

N

R

E
x
R

-

N

P
|
P

S

G

Q

A

V

V

I

V

S

R

E

H

I

I

R

L

S

S

A

R

L

L

binding adenosine-5'-triphosphate: G11 (= G12), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110), R152 (≠ E156), P154 (= P157)

4bqsA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with adp and a shikimic acid derivative. (see paper)
29% identity, 93% coverage: 7:168/174 of query aligns to 6:165/169 of 4bqsA

query
sites
4bqsA

L

L

I

V

G

G

P

L

R
x
P

G
|
G

C

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

M

R

A

R

L

L

A

A

D

K

S

A

L

L

N

G

R

V

R

G

F

L

V

L

D

D

T

T

D
|
D

Q

V

W

A

L

I

Q

E

S

Q

Q

R

L

T

N

G

M

R

T

S

V

I

A

A

E

D

I

I

V

F

E

A

R

T

E

D

E

G

W

E

A

Q

G

E

F

F

R

R

A

R

R

I

E

E

T

E

A

D

A

V

L

V

E

R

A

A

V

A

T

L

A

A

P

D

-

H

S

D

T

G

V

V

I

L

A

S

T

L

G

G

I

A

I

V

L

T

T

S

E

P

F

G

N

V

R

R

H

A

F

A

M

L

Q

A

N

G

N

H

G

-

I

T

V

V

Y

Y

L

L

C

E

A

I

P

S

V

A

S

A

V

E

L

G

V

V

N

R

R
|
R

L

T

Q

G

A

G

A

-

P

-

E

-

E

N

D

T

L

V

R

R

P

P

T

L

L

L

T

A

G

G

K

P

P

D

L

R

S

A

E

E

E

K

V

Y

Q

R

E

A

V

L

L

M

E

A

E

K

R
|
R

D

A

A

P

L

L

Y

Y

R

R

E

R

V

V

A

A

H

T

I

M

I

R

I

V

D

D

A

T

T

N

N

R

E
x
R

-

N

P
|
P

S

G

Q

A

V

V

I

V

S

R

E

H

I

I

R

L

S

S

A

R

L

L

binding adenosine-5'-diphosphate: P10 (≠ R11), G11 (= G12), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110), R152 (≠ E156), P154 (= P157)
binding (1R,6R,10S)-6,10-dihydroxy-2-oxabicyclo[4.3.1]deca-4(Z),7-diene-8-carboxylic acid: D33 (= D34), R135 (= R139)

2iyyA Shikimate kinase from mycobacterium tuberculosis in complex with shikimate-3-phosphate and so4 (see paper)
29% identity, 93% coverage: 7:168/174 of query aligns to 6:165/169 of 2iyyA

query
sites
2iyyA

L

L

I

V

G

G

P

L

R

P

G

G

C

S

G

G

K
|
K

T

S

T

T

V

I

G

G

M

R

A

R

L

L

A

A

D

K

S

A

L

L

N

G

R

V

R

G

F

L

V

L

D

D

T

T

D
|
D

Q

V

W

A

L

I

Q
x
E

S

Q

Q

R

L

T

N

G

M

R

T
x
S

V
x
I

A

A

E

D

I

I

V

F

E

A

R

T

E

D

E

G

W

E

A

Q

G

E

F

F

R
|
R

A

R

R

I

E

E

T

E

A

D

A

V

L

V

E

R

A

A

V

A

T

L

A

A

P

D

-

H

S

D

T

G

V

V

I

L

A

S

T

L

G

G

G
|
G

I

A

I

V

L

T

T

S

E

P

F

G

N

V

R

R

H

A

F

A

M

L

Q

A

N

G

N

H

G

-

I

T

V

V

Y

Y

L

L

C

E

A

I

P

S

V

A

S

A

V

E

L

G

V

V

N

R

R

R

L

T

Q

G

A

G

A

-

P

-

E

-

E

N

D

T

L

V

R

R

P

P

T
x
L

L

L

T

A

G

G

K

P

P

D

L

R

S

A

E

E

E

K

V

Y

Q

R

E

A

V

L

L

M

E

A

E

K

R
|
R

D

A

A

P

L

L

Y

Y

R

R

E

R

V

V

A

A

H

T

I

M

I

R

I

V

D

D

A

T

T

N

N

R

E

R

-

N

P

P

S

G

Q

A

V

V

I

V

S

R

E

H

I

I

R

L

S

S

A

R

L

L

binding magnesium ion: E37 (≠ Q38), S43 (≠ T44), I44 (≠ V45)
binding phosphate ion: K14 (= K15), G79 (= G79)
binding shikimate-3-phosphate: K14 (= K15), D33 (= D34), R57 (= R58), G79 (= G79), G80 (= G80), L118 (≠ T122), R135 (= R139)

3bafA Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with amp-pnp
29% identity, 93% coverage: 7:168/174 of query aligns to 6:165/165 of 3bafA

query
sites
3bafA

L

L

I

V

G

G

P

L

R
x
P

G

G

C

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

M

R

A

R

L

L

A

A

D

K

S

A

L

L

N

G

R

V

R

G

F

L

V

L

D

D

T

T

D
|
D

Q

V

W

A

L

I

Q

E

S

Q

Q

R

L

T

N

G

M

R

T

S

V

I

A

A

E

D

I

I

V

F

E

A

R

T

E

D

E

G

W

E

A

Q

G

E

F

F

R

R

A

R

R

I

E

E

T

E

A

D

A

V

L

V

E

R

A

A

V

A

T

L

A

A

P

D

-

H

S

D

T

G

V

V

I

L

A

S

T

L

G

G

I

A

I

V

L

T

T

S

E

P

F

G

N

V

R

R

H

A

F

A

M

L

Q

A

N

G

N

H

G

-

I

T

V

V

Y

Y

L

L

C

E

A

I

P

S

V

A

S

A

V

E

L

G

V

V

N

R

R
|
R

L

T

Q

G

A

G

A

-

P

-

E

-

E

N

D

T

L

V

R
|
R

P

P

T

L

L

L

T

A

G

G

K

P

P

D

L

R

S

A

E

E

E

K

V

Y

Q

R

E

A

V

L

L

M

E

A

E

K

R
|
R

D

A

A

P

L

L

Y

Y

R

R

E

R

V

V

A

A

H

T

I

M

I

R

I

V

D

D

A

T

T

N

N

R

E

R

-

N

P

P

S

G

Q

A

V

V

I

V

S

R

E

H

I

I

R

L

S

S

A

R

L

L

binding phosphoaminophosphonic acid-adenylate ester: P10 (≠ R11), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110), R116 (= R120)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D34), R135 (= R139)

2dfnA Structure of shikimate kinase from mycobacterium tuberculosis complexed with adp and shikimate at 1.9 angstrons of resolution (see paper)
29% identity, 93% coverage: 7:168/174 of query aligns to 6:165/165 of 2dfnA

query
sites
2dfnA

L

L

I

V

G

G

P

L

R
x
P

G
|
G

C
x
S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

M

R

A

R

L

L

A

A

D

K

S

A

L

L

N

G

R

V

R

G

F

L

V

L

D

D

T

T

D
|
D

Q

V

W

A

L

I

Q

E

S

Q

Q

R

L

T

N

G

M

R

T

S

V
x
I

A

A

E

D

I

I

V

F

E

A

R

T

E

D

E

G

W

E

A

Q

G

E

F
|
F

R
|
R

A

R

R

I

E

E

T

E

A

D

A

V

L

V

E

R

A

A

V

A

T

L

A

A

P

D

-

H

S

D

T

G

V

V

I

L

A

S

T

L

G
|
G

I

A

I

V

L

T

T

S

E

P

F

G

N

V

R

R

H

A

F

A

M

L

Q

A

N

G

N

H

G

-

I

T

V

V

Y

Y

L

L

C

E

A

I

P

S

V

A

S

A

V

E

L

G

V

V

N

R

R
|
R

L

T

Q

G

A

G

A

-

P

-

E

-

E

N

D

T

L

V

R
|
R

P

P

T

L

L

L

T

A

G

G

K

P

P

D

L

R

S

A

E

E

E

K

V

Y

Q

R

E

A

V

L

L

M

E

A

E

K

R
|
R

D

A

A

P

L

L

Y

Y

R

R

E

R

V

V

A

A

H

T

I

M

I

R

I

V

D

D

A

T

T

N

N

R

E
x
R

-

N

P
|
P

S

G

Q

A

V

V

I

V

S

R

E

H

I

I

R

L

S

S

A

R

L

L

binding adenosine-5'-diphosphate: G11 (= G12), S12 (≠ C13), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110), R116 (= R120), R152 (≠ E156), P154 (= P157)
binding chloride ion: P10 (≠ R11), K14 (= K15), G79 (= G79)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D34), I44 (≠ V45), F56 (= F57), R57 (= R58), G78 (= G78), G79 (= G79), G80 (= G80), R135 (= R139)

1zyuA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and amppcp at 2.85 angstrom resolution (see paper)
29% identity, 93% coverage: 7:168/174 of query aligns to 6:165/168 of 1zyuA

query
sites
1zyuA

L

L

I

V

G

G

P

L

R
x
P

G
|
G

C

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

M

R

A

R

L

L

A

A

D

K

S

A

L

L

N

G

R

V

R

G

F

L

V

L

D

D

T

T

D
|
D

Q

V

W

A

L

I

Q

E

S

Q

Q

R

L

T

N

G

M

R

T

S

V

I

A

A

E

D

I

I

V

F

E

A

R

T

E

D

E

G

W

E

A

Q

G

E

F

F

R
|
R

A

R

R

I

E

E

T

E

A

D

A

V

L

V

E

R

A

A

V

A

T

L

A

A

P

D

-

H

S

D

T

G

V

V

I

L

A

S

T

L

G

G

G
|
G

I

A

I

V

L

T

T

S

E

P

F

G

N

V

R

R

H

A

F

A

M

L

Q

A

N

G

N

H

G

-

I

T

V

V

Y

Y

L

L

C

E

A

I

P

S

V

A

S

A

V

E

L

G

V

V

N

R

R
|
R

L

T

Q

G

A

G

A

-

P

-

E

-

E

N

D

T

L

V

R

R

P
|
P

T
x
L

L

L

T

A

G

G

K

P

P

D

L

R

S

A

E

E

E

K

V

Y

Q

R

E

A

V

L

L

M

E

A

E

K

R
|
R

D

A

A

P

L

L

Y

Y

R

R

E

R

V

V

A

A

H

T

I

M

I

R

I

V

D

D

A
x
T

T

N

N

R

E
x
R

-

N

P
|
P

S

G

Q

A

V

V

I

V

S

R

E

H

I

I

R

L

S

S

A

R

L

L

binding phosphomethylphosphonic acid adenylate ester: P10 (≠ R11), G11 (= G12), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110), T149 (≠ A153), R152 (≠ E156), P154 (= P157)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D34), R57 (= R58), G80 (= G80), P117 (= P121), L118 (≠ T122), R135 (= R139)

1we2A Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with mgadp and shikimic acid (see paper)
29% identity, 93% coverage: 7:168/174 of query aligns to 6:165/165 of 1we2A

query
sites
1we2A

L

L

I

V

G

G

P

L

R
x
P

G
|
G

C

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

M

R

A

R

L

L

A

A

D

K

S

A

L

L

N

G

R

V

R

G

F

L

V

L

D

D

T

T

D
|
D

Q

V

W

A

L

I

Q

E

S

Q

Q

R

L

T

N

G

M

R

T

S

V
x
I

A

A

E

D

I

I

V
x
F

E

A

R

T

E

D

E

G

W

E

A

Q

G

E

F

F

R

R

A

R

R

I

E

E

T

E

A

D

A

V

L

V

E

R

A

A

V

A

T

L

A

A

P

D

-

H

S

D

T

G

V

V

I

L

A

S

T

L

G
|
G

I

A

I

V

L

T

T

S

E

P

F

G

N

V

R

R

H

A

F

A

M

L

Q

A

N

G

N

H

G

-

I

T

V

V

Y

Y

L

L

C

E

A

I

P

S

V

A

S

A

V

E

L

G

V

V

N

R

R
|
R

L

T

Q

G

A

G

A

-

P

-

E

-

E

N

D

T

L

V

R
|
R

P

P

T
x
L

L

L

T

A

G

G

K

P

P

D

L

R

S

A

E

E

E

K

V

Y

Q

R

E

A

V

L

L

M

E

A

E

K

R
|
R

D

A

A

P

L

L

Y

Y

R

R

E

R

V

V

A

A

H

T

I

M

I

R

I

V

D

D

A

T

T

N

N

R

E

R

-

N

P

P

S

G

Q

A

V

V

I

V

S

R

E

H

I

I

R

L

S

S

A

R

L

L

binding adenosine-5'-diphosphate: P10 (≠ R11), G11 (= G12), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110), R116 (= R120)
binding 3-dehydroshikimate: D33 (= D34), I44 (≠ V45), F48 (≠ V49), G78 (= G78), G79 (= G79), G80 (= G80), L118 (≠ T122), R135 (= R139)

2iyvA Shikimate kinase from mycobacterium tuberculosis in complex with adp, open lid (conf. B) (see paper)
29% identity, 93% coverage: 7:168/174 of query aligns to 6:165/179 of 2iyvA

query
sites
2iyvA

L

L

I

V

G

G

P

L

R

P

G
|
G

C

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

M

R

A

R

L

L

A

A

D

K

S

A

L

L

N

G

R

V

R

G

F

L

V

L

D

D

T

T

D

D

Q

V

W

A

L

I

Q

E

S

Q

Q

R

L

T

N

G

M

R

T

S

V

I

A

A

E

D

I

I

V

F

E

A

R

T

E

D

E

G

W

E

A

Q

G

E

F

F

R

R

A

R

R

I

E

E

T

E

A

D

A

V

L

V

E

R

A

A

V

A

T

L

A

A

P

D

-

H

S

D

T

G

V

V

I

L

A

S

T

L

G

G

I

A

I

V

L

T

T

S

E

P

F

G

N

V

R

R

H

A

F

A

M

L

Q

A

N

G

N

H

G

-

I

T

V

V

Y

Y

L

L

C

E

A

I

P

S

V

A

S

A

V

E

L

G

V

V

N

R

R
|
R

L

T

Q

G

A

G

A

-

P

-

E

-

E

N

D

T

L

V

R

R

P

P

T

L

L

L

T

A

G

G

K

P

P

D

L

R

S

A

E

E

E

K

V

Y

Q

R

E

A

V

L

L

M

E

A

E

K

R

R

D

A

A

P

L

L

Y

Y

R

R

E

R

V

V

A

A

H

T

I

M

I

R

I

V

D

D

A

T

T

N

N

R

E
x
R

-

N

P
|
P

S

G

Q

A

V

V

I

V

S

R

E

H

I

I

R

L

S

S

A

R

L

L

binding adenosine-5'-diphosphate: G11 (= G12), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110), R152 (≠ E156), P154 (= P157)

1u8aA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and adp at 2.15 angstrom resolution (see paper)
29% identity, 93% coverage: 7:168/174 of query aligns to 6:163/163 of 1u8aA

query
sites
1u8aA

L

L

I

V

G

G

P

L

R

P

G
|
G

C

S

G
|
G

K
|
K

T
x
S

T
|
T

V

I

G

G

M

R

A

R

L

L

A

A

D

K

S

A

L

L

N

G

R

V

R

G

F

L

V

L

D

D

T

T

D
|
D

Q

V

W

A

L

I

Q

E

S

Q

Q

R

L

T

N

G

M

R

T

S

V

I

A

A

E

D

I

I

V
x
F

E

A

R

T

E

D

E

G

W

E

A

Q

G

E

F

F

R
|
R

A

R

R

I

E

E

T

E

A

D

A

V

L

V

E

R

A

A

V

A

T

L

A

A

P

D

-

H

S

D

T

G

V

V

I

L

A

S

T

L

G

G

G
|
G

I

A

I

V

L

T

T

S

E

P

F

G

N

V

R

R

H

A

F

A

M

L

Q

A

N

G

N

H

G

-

I

T

V

V

Y

Y

L

L

C

E

A

I

P

S

V

A

S

A

V

E

L

G

V

V

N

R

R
|
R

L

T

Q

G

A

G

A

-

P

-

E

-

D

-

L

V

R

R

P

P

T
x
L

L

L

T

A

G

G

K

P

P

D

L

R

S

A

E

E

E

K

V

Y

Q

R

E

A

V

L

L

M

E

A

E

K

R
|
R

D

A

A

P

L

L

Y

Y

R

R

E

R

V

V

A

A

H

T

I

M

I

R

I

V

D

D

A

T

T

N

N

R

E
x
R

-

N

P
|
P

S

G

Q

A

V

V

I

V

S

R

E

H

I

I

R

L

S

S

A

R

L

L

binding adenosine-5'-diphosphate: G11 (= G12), G13 (= G14), K14 (= K15), S15 (≠ T16), T16 (= T17), R109 (= R110), R150 (≠ E156), P152 (= P157)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D34), F48 (≠ V49), R57 (= R58), G79 (= G79), G80 (= G80), L116 (≠ T122), R133 (= R139)

7tbvA Crystal structure of the shikimate kinase + 3-dehydroquinate dehydratase + 3-dehydroshikimate dehydrogenase domains of aro1 from candida albicans (see paper)
30% identity, 84% coverage: 3:148/174 of query aligns to 4:134/682 of 7tbvA

query
sites
7tbvA

Q

K

P

S

L

I

F

I

L

V

I

I

G

G

P

M

R

R

G

G

C

T

G

G

K

K

T

S

T

T

V

L

G

S

M

E

A

W

L

L

A

A

D

S

S

F

L

L

N

G

R

F

R

K

F

M

V

L

D

D

T

M

D

D

Q

K

W

Y

L

L

Q

E

S

E

Q

K

L

L

N

G

M

T

T

G

V

I

A

K

E

S

I

L

V

I

E

K

R

A

E

K

E

G

W

W

A

E

G

Y

F

F

R

R

A

Q

R

E

E

E

T

A

-

I

A

V

A

A

L

K

E

E

A

C

V

F

T

T

A

K

P

F

S

S

T

K

-

G

-

Y

V

V

I

L

A

S

T

T

G

G

I

I

I

V

L

E

T

G

E

E

F

D

N

A

R

R

H

Q

-

Q

-

L

-

K

-

S

F

Y

M

A

Q

D

N

N

N

G

G

G

I

I

V

V

V

L

Y

H

L

L

C

-

A

-

P

-

V

-

S

-

V

-

L

-

V

-

N

-

R

-

L

-

Q

-

A

-

P

-

E

H

E

R

D

D

L

L

R

D

P

E

T

-

L

-

T

-

G

-

K

-

P

-

L

-

S

S

E

S

E

E

V

V

Q

Q

E

E

V

V

L

W

E

L

E

R

R

R

D

E

A

K

L

W

Y

Y

R

H

E

E

V

C

A

S

H

N

Sites not aligning to the query:

binding magnesium ion: 300, 302, 329

Query Sequence

>14526 FitnessBrowser__Keio:14526
MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMTVAEIVEREEWAGFRAR
ETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCAPVSVLVNRLQAAPEEDLR
PTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQVISEIRSALAQTINC

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory