SitesBLAST

C204 (≠ L183) mutation to S: Destabilizes the protein structure; when associated with S-326.
C326 (≠ A306) mutation to S: Destabilizes the protein structure; when associated with S-204.
D419 (= D410) mutation to A: Still allows binding to Mlc.
C421 (= C412) modified: Phosphocysteine; mutation to D: Still allows binding to Mlc. Derepression of Mlc targets becomes constitutive, i.e. independent of PTS phosphorylation.; mutation to S: Unable to be phosphorylated and to catalyze the phosphoryl exchange between glucose and glucose 6-phosphate at equilibrium. Remains susceptible to CdiA toxin from E.coli strain 3006. Still allows binding to Mlc. Derepression of Mlc targets becomes constitutive, i.e. independent of PTS phosphorylation.
I422 (= I413) mutation to A: Still allows binding to Mlc.
T423 (= T414) mutation to A: Decreases Mlc binding.
R424 (= R415) mutation R->A,H: Cannot bind Mlc. Destroys the ability of Cys-421 to be phosphorylated in vitro.; mutation to K: Cannot bind Mlc. Cys-421 can be phosphorylated in vitro.
R426 (= R417) mutation to A: Still allows binding to Mlc. Cys-421 is weakly phosphorylated in vitro.
V449 (= V440) mutation to Q: Interaction with Mlc is hardly detectable.
A451 (≠ N443) mutation to F: The complex with Mlc shows much weaker association than the wild-type.
Q456 (= Q448) mutation to A: Decreases Mlc binding. Interaction with Mlc is hardly detectable.
I458 (= I450) mutation to A: Still allows binding to Mlc.

8qsrA Cryo-em structure of the glucose-specific pts transporter iicb from e. Coli in the inward-facing conformation
42% identity, 56% coverage: 8:369/648 of query aligns to 7:383/383 of 8qsrA

query
sites
8qsrA

Q

Q

R

K

L

V

G

G

R

K

A

S

L

L

Q

M

L

L

P

P

I

V

A

S

V

V

L

L

P

P

V

I

A

A

A

G

L

I

L

L

R

G

F

V

G

G

Q

S

P

A

D

N

L

F

-

S

-

W

-

L

-

P

-

A

L

V

N

V

V

S

A

H

F

V

I

M

A

A

Q

E

A

A

G

G

A

S

I

V

F

F

D

A

N

N

L

M

A

P

L

L

I

I

F

F

A

A

I

I

G

G

V

V

A

A

S

L

S

G

W

F

S

T

K

N

D

N

S

D

A

-

G

G

A

V

A

S

A

A

L

L

A

A

G

A

A

V

V

V

G

A

Y

Y

F

G

V

I

L

M

T

V

K

K

A

T

M

M

V

A

T

V

I

V

N

A

P

P

E

L

I

V

-

L

-

H

-

L

-

P

-

A

-

E

-

I

-

A

-

S

-

K

-

H

-

L

-

A

N

D

M

T

G

G

V

V

L

L

A

G

G

G

I

I

T

S

G

G

L

A

V

I

G

A

A

Y

A

M

Y

F

N

N

R

R

W

F

S

Y

D

R

I

I

K

K

L

L

P

P

D

E

F

Y

L

L

S

G

F

F

G

A

G

G

K

K

R

R

F

F

V

V

P

P

I

I

A

I

T

S

G

G

F

L

F

A

C

A

L

I

V

F

L

T

A

G

A

V

I

V

F

L

G

S

Y

F

V

I

W

W

P

P

V

I

Q

G

H

S

A

A

I

I

H

Q

A

T

G

F

G

S

E

Q

W

W

I

A

V

A

S

Y

A

Q

G

N

-

P

A

V

L

V

G

A

S

F

G

G

I

I

F

Y

G

G

F

F

I

I

N

E

R
|
R

L

C

L

L

I

V

P

P

T

F

G

G

L

L

H

H

Q

H

V

I

L

W

N

N

T

V

I

P

A

F

W

Q

F

M

Q

Q

I

I

G

G

E

E

F

Y

T

T

N

N

A

A

G

G

T

Q

V

V

F

F

H

H

G

G

D

D

I

I

N

P

R

R

F

Y

Y

M

A

A

G

G

D

D

G

P

T

T

A

A

G

G

M

K

F

-

M

L

S

S

G

G

F

G

F

F

P

L

I

F

M
x
K

M

M

F

Y

G

G

L

L

P

P

G

A

A

A

L

I

A

A

M

I

Y

W

F

H

A

S

A

A

P

K

K

P

E

E

R

N

R

R

P

A

M

K

V

V

G

G

M

I

L

M

L

I

S

S

V

A

A

A

V

L

T

T

A

S

F

F

L

L

T

T

G

G

V

I

T
|
T

E
|
E

P

P

L

I

E

E

F

F

L

S

F

F

M

M

F

F

L

V

A

A

P

P

L

I

L

L

Y

Y

L

I

H

H

A

A

L

I

L

L

T

A

G

G

I

L

S

A

L

F

F

P

V

I

A

C

T

I

L

L

G

G

I

M

H

R

A

D

G

G

F

T

S

S

F
|
F

S

S

A

H

G

G

A

L

I

I

D

D

Y

F

A

I

L

V

M

-

Y

-

N

-

L

L

P

S

A

G

A

N

S

S

Q

S

N

K

V

L

W

W

M

L

L

F

L

P

V

I

M

V

G

G

V

I

I

G

F

Y

F

A

A

I

I

V

Y

Y

F

Y

V

T

V

I

F

F

S

R

L

V

V

L

I

I

R

K

M

A

F

L

N

D

L

L

K

K

T

T

P

P

G

G

R

R

binding beta-D-glucopyranose: R200 (= R182), K254 (≠ M237), T294 (= T277), E295 (= E278), F334 (= F317)

6bvgA Crystal structure of bcmalt t280c-e54c crosslinked by divalent mercury (see paper)
32% identity, 56% coverage: 6:369/648 of query aligns to 4:442/447 of 6bvgA

query
sites
6bvgA

F

F

F

W

Q

Q

R

K

L

F

G

G

R

K

A

A

L

L

Q

L

L

V

P

V

I

V

A

A

V

V

L

M

P

P

V

A

A

A

A

G

L

L

L

M

L

I

R

S

F

I

G

G

Q

K

-

L

-

I

-

G

-

M

-

S

-

A

P

G

D

D

L

I

L

N

N

A

V

V

A

H

F

T

I

I

A

A

Q

R

A

V

-

M

-

C

-

D

-

I

G

G

G

W

A

A

I

I

F

I

D

T

N

N

L

L

A

H

L

I

I

L

F

F

A

A

I

V

G

A

V

I

A

G

S

G

S

S

W

W

S

A

K

K

D

D

S

R

A

A

G

G

A

G

A

A

A

F

L

A

A

A

G

L

A

L

V

A

G

F

Y

V

F

L

V

T

-

N

-

R

L

I

T

T

K

G

A

A

M

I

V

F

T

G

I

V

N

N

-

A

-

E

-

M

-

L

-

A

-

D

-

S

-

K

-

A

-

K

-

V

-

S

-

V

-

L

-

A

-

G

-

D

-

L

-

I

-

V

-

K

-

D

-

Y

-

F

-

T

-

S

-

V

-

L

-

G

-

A

P

P

E

A

I

L

N

N

M

M

G

G

V

V

L

F

A

V

G

G

I

I

T

T

G

G

L

F

V

L

G

G

G

A

A

T

A

L

Y

Y

N

N

R

K

W

Y

S

Y

D

N

I

Y

-

N

K

K

L

L

P

P

D

Q

F

A

L

L

S

A

F

F

G

N

G

G

K

K

R

R

F

F

V

V

P

P

I

F

A

V

T

V

G

I

F

V

F

W

C

S

L

T

V

V

L

T

A

A

A

I

I

V

F

L

G

S

Y

L

V

L

W

W

P

P

P

F

V

I

Q

Q

H

S

A

G

I

L

H

N

A

E

G

F

G

G

E

R

W

W

I

I

V

A

-

A

-

S

-

K

-

D

S

S

A

A

G

P

A

I

L

V

G

A

S

P

G

F

I

V

F

Y

G

G

F

T

I

L

N
x
E

R
|
R

L

L

I

L

P

P

T

F

G

G

L

L

H
|
H

Q
x
H

V

M

L

L

N
x
T

T

I

-

P

I

M

A

N

W
x
Y

F

T

Q

E

I

L

-

G

G

G

E

T

F

Y

T

T

-

M

-

L

-

T

-

G

N

S

A

K

A

V

G

G

T

Q

V

V

F

V

H

A

G

G

-

Q

-

D

-

P

-

L

-

W

-

L

-

A

-

W

-

I

-

C

D

D

I

L

N

N

R

N

F

L

Y

L

A

A

-

N

G

G

D

D

G

T

T

K

A

A

-

Y

-

N

-

D

-

L

-

N

-

V

-

P

G

A

M

R

F

F

M
x
K

S

A

G

G

F

Q

F

V

P

I

I

G

M

S

M

T

F

A

G

A

L

L

P

M

G

G

A

I

A

A

L

F

A

A

M

M

Y

F

F

R

A

N

A

V

P

D

K

K

E

E

R

K

R

R

P

A

M

K

V

Y

G

K

G

P

M

M

L

F

L

L

S

S

V

A

A

A

V

L

T

A

A

V

F

F

L

L

T

T

G

G

V

V

T
|
T

E
|
E

P

P

L

I

E

E

F

F

L

M

F

F

M

M

F

F

L

I

A

A

P

P

L

V

L

L

Y

Y

L

V

H

Y

A

A

L

I

L

T

T

T

G

G

I

L

S

A

L

F

F

A

V

L

A

A

T

D

L

L

-

I

-

N

L

L

G

R

I

V

H

H

A

A

G

-

F
|
F

S

G

F

F

S

I

A

E

G

L

A

I

I

T

D

R

Y

T

A

P

L

M

M

M

Y

V

N

N

L

-

P

A

A

G

A

L

S

T

Q

R

N

D

V

L

W

I

M

N

L

F

L

V

V

I

M

V

G

S

V

L

I

V

F

F

A

G

I

L

Y

N

F

F

V

T

V

L

F

F

S

N

L

F

V

L

I

I

R

K

M

K

F

F

N

N

L

L

K

P

T

T

P

P

G

G

R

R

binding alpha-D-glucopyranose: E227 (≠ N181), R228 (= R182), H236 (= H190), H237 (≠ Q191), H237 (≠ Q191), T240 (≠ N194), Y245 (≠ W198), K303 (≠ M230), T350 (= T277), E351 (= E278), F389 (= F315)

1glcF Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
50% identity, 24% coverage: 496:648/648 of query aligns to 10:161/161 of 1glcF

query
sites
1glcF

V

V

S

S

I

T

A

I

E

E

L

I

V

I

S

A

P

P

I

L

T

S

G

G

D

E

V

I

V

V

A

N

L

I

D

E

Q

D

V

V

P

P

D

D

E

V

A

V

F

F

A

A

S

E

K

K

A

I

V

V

G

G

D

D

G

G

V

I

A

A

V

I

K

K

P

P

T

T

D

G

K

N

I

K

V

M

V

V

S

A

P

P

A

V

A

D

G

G

T

T

I

I

V

G

K

K

I

I

F

F

N

E

T

T

N

N

H
|
H

A

A

F

F

C

S

L

I

E

E

T

S

E

D

K

S

G

G

A

V

E

E

I

L

V

F

V

V

H
|
H

M

F

G

G

I

I

D

D

T

T

V

V

A

E

L

L

E

K

G

G

K

E

G

G

F

F

K

K

R

R

L

I

V

A

E

E

G

G

A

Q

Q

R

V

V

S

K

A

V

G

G

Q

D

P

T

I

V

L

I

E

E

M

F

D

D

L

L

D

P

Y

L

L

L

N

E

A

E

N

K

A

A

R

K

S

S

M

T

I

L

S

T

P

P

V

V

C

I

S

S

N

N

I

M

D

D

D

E

F

I

S

K

G

E

L

L

I

-

I

I

K

K

A

L

Q

S

G

G

H

S

I

V

V

T

A

V

G

G

Q

E

T

T

P

P

L

V

Y

I

E

R

I

I

K

K

binding zinc ion: H68 (= H554), H83 (= H569)

P69783 PTS system glucose-specific EIIA component; EIIA-Glc; EIII-Glc; Glucose-specific phosphotransferase enzyme IIA component from Escherichia coli (strain K12) (see 6 papers)
50% identity, 23% coverage: 500:648/648 of query aligns to 22:169/169 of P69783

query
sites
P69783

E

E

L

I

V

I

S

A

P

P

I

L

T

S

G

G

D

E

V

I

V

V

A

N

L

I

D

E

Q

D

V

V

P

P

D

D

E

V

A

V

F

F

A

A

S

E

K

K

A

I

V

V

G

G

D

D

G

G

V

I

A

A

V

I

K

K

P

P

T

T

D

G

K

N

I

K

V

M

V

V

S

A

P

P

A

V

A

D

G

G

T

T

I

I

V

G

K

K

I

I

F

F

N

E

T

T

N

N

H
|
H

A

A

F

F

C

S

L

I

E

E

T

S

E

D

K

S

G

G

A

V

E

E

I

L

V

F

V

V

H
|
H

M

F

G

G

I

I

D

D

T

T

V

V

A

E

L

L

E

K

G

G

K

E

G

G

F

F

K

K

R

R

L

I

V

A

E

E

G

G

A

Q

Q

R

V

V

S

K

A

V

G

G

Q

D

P

T

I

V

L

I

E

E

M

F

D

D

L

L

D

P

Y

L

L

L

N

E

A

E

N

K

A

A

R

K

S

S

M

T

I

L

S

T

P

P

V

V

C

I

S

S

N

N

I

M

D

D

D

E

F

I

S

K

G

E

L

L

I

-

I

I

K

K

A

L

Q

S

G

G

H

S

I

V

V

T

A

V

G

G

Q

E

T

T

P

P

L

V

Y

I

E

R

I

I

K

K

H76 (= H554) Important for phospho-donor activity; binding Zn(2+); mutation to Q: Unable to transfer phosphoryl group.
H91 (= H569) active site, Tele-phosphohistidine intermediate; for EIIA activity; binding Zn(2+); modified: Phosphohistidine; by HPr; mutation to Q: Unable to be phosphorylated by Hpr.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
4 F→W: Same activity as the wild-type.

1o2fA Complex of enzyme iiaglc and iibglc phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
50% identity, 23% coverage: 500:648/648 of query aligns to 3:150/150 of 1o2fA

query
sites
1o2fA

E

E

L

I

V

I

S

A

P

P

I

L

T

S

G

G

D

E

V

I

V

V

A

N

L

I

D

E

Q

D

V

V

P

P

D

D

E

V

A

V

F

F

A

A

S

E

K

K

A

I

V

V

G

G

D

D

G

G

V

I

A

A

V

I

K

K

P

P

T

T

D

G

K

N

I

K

V

M

V

V

S

A

P

P

A

V

A

D

G

G

T

T

I

I

V

G

K

K

I

I

F

F

N

E

T
|
T

N

N

H
|
H

A

A

F

F

C

S

L

I

E

E

T

S

E

D

K

S

G

G

A

V

E

E

I

L

V

F

V

V

H
|
H

M

F

G

G

I

I

D
|
D

T

T

V

V

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active site: T55 (= T552), H57 (= H554), H72 (= H569)
binding phosphite ion: H72 (= H569), D76 (= D573)

1o2fB Complex of enzyme iiaglc and iibglc phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
39% identity, 12% coverage: 391:466/648 of query aligns to 1:75/77 of 1o2fB

query
sites
1o2fB

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binding phosphite ion: C22 (= C412), I23 (= I413), T24 (= T414)

Query Sequence

>14809 FitnessBrowser__Keio:14809
MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQAGGAIFDNLALIFAIGV
ASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGIITGLVGGAAYNRWSDIKL
PDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIHAGGEWIVSAGALGSGIFGFI
NRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINRFYAGDGTAGMFMSGFFPIMMFG
LPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGVTEPLEFLFMFLAPLLYLLHALLTG
ISLFVATLLGIHAGFSFSAGAIDYALMYNLPAASQNVWMLLVMGVIFFAIYFVVFSLVIR
MFNLKTPGREDKEDEIVTEEANSNTEEGLTQLATNYIAAVGGTDNLKAIDACITRLRLTV
ADSARVNDTMCKRLGASGVVKLNKQTIQVIVGAKAESIGDAMKKVVARGPVAAASAEATP
ATAAPVAKPQAVPNAVSIAELVSPITGDVVALDQVPDEAFASKAVGDGVAVKPTDKIVVS
PAAGTIVKIFNTNHAFCLETEKGAEIVVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILE
MDLDYLNANARSMISPVVCSNIDDFSGLIIKAQGHIVAGQTPLYEIKK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory