SitesBLAST

YI 11:12 (= YI 11:12) binding
DNLCNS 31:36 (= DNLCNS 31:36) binding
DI 58:59 (= DI 58:59) binding
FAGLK 80:84 (= FAGLK 80:84) binding
N99 (= N99) binding
S124 (= S124) binding ; mutation to A: No major structural changes. Catalytic efficiency is very low and affinity binding is 21% of the wild-type enzyme.; mutation to T: No major structural changes. Catalytic efficiency is about 30% of that of the wild-type enzyme, and affinity binding is similar to that of the native enzyme.
Y149 (= Y149) active site, Proton acceptor; binding ; mutation to F: No major structural changes. Catalytic efficiency is very low and affinity binding is 12% of the wild-type enzyme.
K153 (= K153) binding ; mutation to A: Decreases the catalytic activity. Not reduced by sugars in the presence or absence of UMP. It contains very little NADH.; mutation to M: Decreases the catalytic activity. Not reduced by sugars in the presence or absence of UMP. It contains very little NADH.
F178 (= F178) binding
Y299 (= Y299) mutation to C: Loss of epimerase activity with UDP-Gal by almost 5-fold, but it results in a gain of epimerase activity with uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) by 230-fold with minimal changes in its three-dimensional structure.

1kvrA Udp-galactose 4-epimerase complexed with udp-phenol (see paper)
100% identity, 100% coverage: 1:338/338 of query aligns to 1:338/338 of 1kvrA

query
sites
1kvrA

M

M

R

R

V

V

L

L

V

V

T

T

G
|
G

G

G

S

S

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

C

V

V

Q

Q

L

L

Q

Q

N

N

G

G

H

H

D

D

V

V

I

I

L

L

D
|
D

N
|
N

L

L

C
|
C

N
|
N

S
|
S

K

K

R

R

S

S

V

V

L

L

P

P

V

V

I

I

E

E

R

R

L

L

G

G

K

K

H

H

P

P

T

T

F

F

V

V

E

E

G

G

D
|
D

I
|
I

R

R

N

N

E

E

A

A

L

L

M

M

T

T

E

E

I

I

L

L

H

H

D

D

H

H

A

A

I

I

D

D

T

T

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

Q

Q

K

K

P

P

L

L

E

E

Y

Y

D

D

N

N

V

V

N

N

G

G

T

T

L

L

R

R

L

L

I

I

S

S

A

A

M

M

R

R

A

A

N

N

V

V

K

K

N

N

F

F

I

I

F

F

S
|
S

S
x
A

A
|
A

T
|
T

V

V

Y

Y

G

G

D

D

Q

Q

P

P

K

K

I

I

P

P

Y

Y

V

V

E

E

S

S

F

F

P

P

T

T

G

G

T

T

P

P

Q

Q

S

S

P

P

Y
|
Y

G

G

K

K

S

S

K
|
K

L

L

M

M

V

V

E

E

Q

Q

I

I

L

L

T

T

D

D

L

L

Q

Q

K

K

A

A

Q

Q

P

P

D

D

W

W

S

S

I

I

A

A

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

P

S

S

G

G

D

D

M
|
M

G

G

E

E

D

D

P

P

Q

Q

G

G

I

I

P

P

N
|
N

L
|
L

M

M

P

P

Y

Y

I

I

A

A

Q

Q

V

V

A

A

V

V

G

G

R

R

D

D

S

S

L

L

A
|
A

I
|
I

F
|
F

G

G

N

N

D

D

Y

Y

P

P

T

T

E

E

D

D

G

G

T

T

G

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

M

M

D

D

L

L

A

A

D

D

G

G

H

H

V

V

A

A

M

M

E

E

K

K

L

L

A

A

N

N

K

K

P

P

G

G

V

V

H

H

I

I

Y

Y

N

N

L

L

G

G

A

A

G

G

V

V

G

G

N

N

S

S

V
|
V

L

L

D

D

V

V

N

N

A

A

F

F

S

S

K

K

A

A

C

C

G

G

K

K

P

P

V

V

N

N

Y

Y

H

H

F

F

A

A

P

P

R

R

R
|
R

E

E

G

G

D
|
D

L

L

P

P

A

A

Y

Y

W

W

A

A

D

D

A

A

S

S

K

K

A

A

D

D

R

R

E

E

L

L

N

N

W

W

R

R

V

V

T

T

R

R

T

T

L

L

D

D

E

E

M

M

A

A

Q

Q

D

D

T

T

W

W

H

H

W

W

Q

Q

S

S

R

R

H

H

P

P

Q

Q

G

G

Y

Y

P

P

D

D

active site: A124 (≠ S124), A125 (= A125), T126 (= T126), Y149 (= Y149), K153 (= K153), M189 (= M189)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (= Y11), I12 (= I12), D31 (= D31), N32 (= N32), C34 (= C34), N35 (= N35), S36 (= S36), D58 (= D58), I59 (= I59), F80 (= F80), A81 (= A81), G82 (= G82), K84 (= K84), S122 (= S122), S123 (= S123), Y149 (= Y149), K153 (= K153), Y177 (= Y177), P180 (= P180)
binding uridine-5'-diphosphate: N179 (= N179), N198 (= N198), N199 (= N199), L200 (= L200), A216 (= A216), I217 (= I217), F218 (= F218), R231 (= R231), Y233 (= Y233), V269 (= V269), R292 (= R292), D295 (= D295)

1a9yA Udp-galactose 4-epimerase mutant s124a/y149f complexed with udp- glucose (see paper)
99% identity, 100% coverage: 1:338/338 of query aligns to 1:338/338 of 1a9yA

query
sites
1a9yA

M

M

R

R

V

V

L

L

V

V

T

T

G
|
G

G

G

S

S

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

C

V

V

Q

Q

L

L

Q

Q

N

N

G

G

H

H

D

D

V

V

I

I

L

L

D
|
D

N
|
N

L

L

C
|
C

N
|
N

S
|
S

K

K

R

R

S

S

V

V

L

L

P

P

V

V

I

I

E

E

R

R

L

L

G

G

K

K

H

H

P

P

T

T

F

F

V

V

E

E

G

G

D
|
D

I
|
I

R

R

N

N

E

E

A

A

L

L

M

M

T

T

E

E

I

I

L

L

H

H

D

D

H

H

A

A

I

I

D

D

T

T

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

Q

Q

K

K

P

P

L

L

E

E

Y

Y

D

D

N

N

V

V

N

N

G

G

T

T

L

L

R

R

L

L

I

I

S

S

A

A

M

M

R

R

A

A

N

N

V

V

K

K

N

N

F

F

I

I

F

F

S
|
S

S
x
A

A
|
A

T
|
T

V

V

Y

Y

G

G

D

D

Q

Q

P

P

K

K

I

I

P

P

Y

Y

V

V

E

E

S

S

F

F

P

P

T

T

G

G

T

T

P

P

Q

Q

S

S

P

P

Y
x
F

G

G

K

K

S

S

K
|
K

L

L

M

M

V

V

E

E

Q

Q

I

I

L

L

T

T

D

D

L

L

Q

Q

K

K

A

A

Q

Q

P

P

D

D

W

W

S

S

I

I

A

A

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

P

S

S

G

G

D

D

M

M

G

G

E

E

D

D

P

P

Q

Q

G

G

I

I

P

P

N

N

N
|
N

L
|
L

M

M

P

P

Y

Y

I

I

A

A

Q

Q

V

V

A

A

V

V

G

G

R

R

D

D

S

S

L

L

A
|
A

I
|
I

F
|
F

G

G

N

N

D

D

Y

Y

P

P

T

T

E

E

D

D

G

G

T

T

G

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

M

M

D

D

L

L

A

A

D

D

G

G

H

H

V

V

A

A

M

M

E

E

K

K

L

L

A

A

N

N

K

K

P

P

G

G

V

V

H

H

I

I

Y

Y

N

N

L

L

G

G

A

A

G

G

V

V

G

G

N

N

S

S

V

V

L

L

D

D

V

V

N

N

A

A

F

F

S

S

K

K

A

A

C

C

G

G

K

K

P

P

V

V

N

N

Y

Y

H

H

F

F

A

A

P

P

R

R

R
|
R

E

E

G

G

D
|
D

L

L

P

P

A

A

Y
|
Y

W

W

A

A

D

D

A

A

S

S

K

K

A

A

D

D

R

R

E

E

L

L

N

N

W

W

R

R

V

V

T

T

R

R

T

T

L

L

D

D

E

E

M

M

A

A

Q

Q

D

D

T

T

W

W

H

H

W

W

Q

Q

S

S

R

R

H

H

P

P

Q

Q

G

G

Y

Y

P

P

D

D

active site: A124 (≠ S124), A125 (= A125), T126 (= T126), F149 (≠ Y149), K153 (= K153)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (= Y11), I12 (= I12), D31 (= D31), N32 (= N32), C34 (= C34), N35 (= N35), S36 (= S36), D58 (= D58), I59 (= I59), F80 (= F80), A81 (= A81), G82 (= G82), K84 (= K84), S122 (= S122), S123 (= S123), F149 (≠ Y149), K153 (= K153), Y177 (= Y177), P180 (= P180)
binding uridine-5'-diphosphate-glucose: A125 (= A125), T126 (= T126), N179 (= N179), N199 (= N199), L200 (= L200), A216 (= A216), I217 (= I217), F218 (= F218), R231 (= R231), Y233 (= Y233), R292 (= R292), D295 (= D295), Y299 (= Y299)

1udaA Structure of udp-galactose-4-epimerase complexed with udp-4-deoxy-4- fluoro-alpha-d-galactose (see paper)
100% identity, 100% coverage: 1:338/338 of query aligns to 1:338/338 of 1udaA

query
sites
1udaA

M

M

R

R

V

V

L

L

V

V

T

T

G
|
G

G

G

S

S

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

C

V

V

Q

Q

L

L

Q

Q

N

N

G

G

H

H

D

D

V

V

I

I

L

L

D
|
D

N
|
N

L
|
L

C
|
C

N
|
N

S
|
S

K

K

R

R

S

S

V

V

L

L

P

P

V

V

I

I

E

E

R

R

L

L

G

G

K

K

H

H

P

P

T

T

F

F

V

V

E

E

G

G

D
|
D

I
|
I

R

R

N

N

E

E

A

A

L

L

M

M

T

T

E

E

I

I

L

L

H

H

D

D

H

H

A

A

I

I

D

D

T

T

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

Q

Q

K

K

P

P

L

L

E

E

Y

Y

D

D

N

N

V

V

N

N

G

G

T

T

L

L

R

R

L

L

I

I

S

S

A

A

M

M

R

R

A

A

N

N

V

V

K

K

N

N

F

F

I

I

F

F

S
|
S

S

S

S
|
S

A
|
A

T
|
T

V

V

Y

Y

G

G

D

D

Q

N

P

P

K

K

I

I

P

P

Y

Y

V

V

E

E

S

S

F

F

P

P

T

T

G

G

T

T

P

P

Q

Q

S

S

P

P

Y
|
Y

G

G

K

K

S

S

K
|
K

L

L

M

M

V

V

E

E

Q

Q

I

I

L

L

T

T

D

D

L

L

Q

Q

K

K

A

A

Q

Q

P

P

D

D

W

W

S

S

I

I

A

A

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

P

S

S

G

G

D

D

M
|
M

G

G

E

E

D

D

P

P

Q

Q

G

G

I

I

P

P

N

N

N
|
N

L
|
L

M

M

P

P

Y

Y

I

I

A

A

Q

Q

V

V

A

A

V

V

G

G

R

R

D

D

S

S

L

L

A
|
A

I
|
I

F
|
F

G

G

N

N

D

D

Y

Y

P

P

T

T

E

E

D

D

G

G

T

T

G

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

M

M

D

D

L

L

A

A

D

D

G

G

H

H

V

V

A

A

M

M

E

E

K

K

L

L

A

A

N

N

K

K

P

P

G

G

V

V

H

H

I

I

Y

Y

N

N

L

L

G

G

A

A

G

G

V

V

G

G

N

N

S

S

V

V

L

L

D

D

V

V

N

N

A

A

F

F

S

S

K

K

A

A

C

C

G

G

K

K

P

P

V

V

N

N

Y

Y

H

H

F

F

A

A

P

P

R

R

R
|
R

E

E

G

G

D
|
D

L

L

P

P

A

A

Y
|
Y

W

W

A

A

D

D

A

A

S

S

K

K

A

A

D

D

R

R

E

E

L

L

N

N

W

W

R

R

V

V

T

T

R

R

T

T

L

L

D

D

E

E

M

M

A

A

Q

Q

D

D

T

T

W

W

H

H

W

W

Q

Q

S

S

R

R

H

H

P

P

Q

Q

G

G

Y

Y

P

P

D

D

active site: S124 (= S124), A125 (= A125), T126 (= T126), Y149 (= Y149), K153 (= K153), M189 (= M189)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (= Y11), I12 (= I12), D31 (= D31), N32 (= N32), L33 (= L33), C34 (= C34), N35 (= N35), S36 (= S36), D58 (= D58), I59 (= I59), F80 (= F80), A81 (= A81), G82 (= G82), K84 (= K84), S122 (= S122), Y149 (= Y149), K153 (= K153), Y177 (= Y177), P180 (= P180)
binding uridine-5'-diphosphate-4-deoxy-4-fluoro-alpha-d-galactose: T126 (= T126), N179 (= N179), N199 (= N199), L200 (= L200), A216 (= A216), I217 (= I217), F218 (= F218), R231 (= R231), Y233 (= Y233), R292 (= R292), D295 (= D295), Y299 (= Y299)

1naiA Udp-galactose 4-epimerase from escherichia coli, oxidized (see paper)
100% identity, 100% coverage: 1:338/338 of query aligns to 1:338/338 of 1naiA

query
sites
1naiA

M

M

R

R

V

V

L

L

V

V

T

T

G
|
G

G

G

S

S

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

C

V

V

Q

Q

L

L

Q

Q

N

N

G

G

H

H

D

D

V

V

I

I

L

L

D
|
D

N
|
N

L
|
L

C
|
C

N
|
N

S
|
S

K

K

R

R

S

S

V

V

L

L

P

P

V

V

I

I

E

E

R

R

L

L

G

G

K

K

H

H

P

P

T

T

F

F

V

V

E

E

G

G

D
|
D

I
|
I

R

R

N

N

E

E

A

A

L

L

M

M

T

T

E

E

I

I

L

L

H

H

D

D

H

H

A

A

I

I

D

D

T

T

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

Q

Q

K

K

P

P

L

L

E

E

Y

Y

D

D

N

N

V

V

N

N

G

G

T

T

L

L

R

R

L

L

I

I

S

S

A

A

M

M

R

R

A

A

N

N

V

V

K

K

N

N

F

F

I

I

F

F

S

S

S
|
S

A
|
A

T
|
T

V

V

Y

Y

G

G

D

D

Q

N

P

P

K

K

I

I

P

P

Y

Y

V

V

E

E

S

S

F

F

P

P

T

T

G

G

T

T

P

P

Q

Q

S

S

P

P

Y
|
Y

G

G

K

K

S

S

K
|
K

L

L

M

M

V

V

E

E

Q

Q

I

I

L

L

T

T

D

D

L

L

Q

Q

K

K

A

A

Q

Q

P

P

D

D

W

W

S

S

I

I

A

A

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

P

S

S

G

G

D

D

M
|
M

G

G

E
|
E

D

D

P
|
P

Q

Q

G

G

I

I

P

P

N

N

N
|
N

L
|
L

M

M

P

P

Y

Y

I

I

A

A

Q

Q

V

V

A

A

V

V

G

G

R

R

D

D

S

S

L
|
L

A
|
A

I

I

F

F

G

G

N

N

D

D

Y

Y

P

P

T

T

E

E

D

D

G

G

T

T

G

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

M

M

D

D

L

L

A

A

D

D

G

G

H

H

V

V

A

A

M

M

E

E

K

K

L

L

A

A

N

N

K

K

P

P

G

G

V

V

H

H

I

I

Y

Y

N

N

L

L

G

G

A

A

G

G

V

V

G

G

N

N

S

S

V

V

L

L

D

D

V

V

N

N

A

A

F

F

S

S

K

K

A

A

C

C

G

G

K

K

P

P

V

V

N

N

Y

Y

H

H

F

F

A

A

P

P

R

R

R
|
R

E

E

G

G

D

D

L

L

P

P

A

A

Y

Y

W

W

A

A

D

D

A

A

S

S

K

K

A

A

D

D

R

R

E

E

L

L

N

N

W

W

R

R

V

V

T

T

R

R

T

T

L

L

D

D

E

E

M

M

A

A

Q

Q

D

D

T

T

W

W

H

H

W

W

Q

Q

S

S

R

R

H

H

P

P

Q

Q

G

G

Y

Y

P

P

D

D

active site: S124 (= S124), A125 (= A125), T126 (= T126), Y149 (= Y149), K153 (= K153), M189 (= M189)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (= Y11), I12 (= I12), D31 (= D31), N32 (= N32), L33 (= L33), C34 (= C34), N35 (= N35), S36 (= S36), D58 (= D58), I59 (= I59), F80 (= F80), A81 (= A81), G82 (= G82), K84 (= K84), Y149 (= Y149), K153 (= K153), Y177 (= Y177), P180 (= P180)
binding 1,3-propandiol: N35 (= N35), K84 (= K84), E191 (= E191), P193 (= P193)
binding uridine-5'-diphosphate: N179 (= N179), N199 (= N199), L200 (= L200), L215 (= L215), A216 (= A216), R231 (= R231), Y233 (= Y233), R292 (= R292)

1lrjA Crystal structure of e. Coli udp-galactose 4-epimerase complexed with udp-n-acetylglucosamine (see paper)
100% identity, 100% coverage: 1:338/338 of query aligns to 1:338/338 of 1lrjA

query
sites
1lrjA

M

M

R

R

V

V

L

L

V

V

T

T

G
|
G

G

G

S

S

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

C

V

V

Q

Q

L

L

Q

Q

N

N

G

G

H

H

D

D

V

V

I

I

L

L

D
|
D

N
|
N

L
|
L

C
|
C

N
|
N

S
|
S

K

K

R

R

S

S

V

V

L

L

P

P

V

V

I

I

E

E

R

R

L

L

G

G

K

K

H

H

P

P

T

T

F

F

V

V

E

E

G

G

D
|
D

I
|
I

R

R

N

N

E

E

A

A

L

L

M

M

T

T

E

E

I

I

L

L

H

H

D

D

H

H

A

A

I

I

D

D

T

T

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L

L

K
|
K

A

A

V
|
V

G

G

E

E

S

S

V

V

Q

Q

K

K

P

P

L

L

E

E

Y

Y

D

D

N

N

V

V

N

N

G

G

T

T

L

L

R

R

L

L

I

I

S

S

A

A

M

M

R

R

A

A

N

N

V

V

K

K

N

N

F

F

I

I

F

F

S

S

S
|
S

A
|
A

T
|
T

V

V

Y

Y

G

G

D

D

Q

N

P

P

K

K

I

I

P

P

Y

Y

V

V

E

E

S

S

F

F

P

P

T

T

G

G

T

T

P

P

Q

Q

S

S

P

P

Y
|
Y

G

G

K

K

S

S

K
|
K

L

L

M

M

V

V

E

E

Q

Q

I

I

L

L

T

T

D

D

L

L

Q

Q

K

K

A

A

Q

Q

P

P

D

D

W

W

S

S

I

I

A

A

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

P

S

S

G

G

D

D

M
|
M

G

G

E

E

D

D

P

P

Q

Q

G

G

I

I

P

P

N

N

L
|
L

M

M

P

P

Y

Y

I

I

A

A

Q

Q

V

V

A

A

V

V

G

G

R

R

D

D

S

S

L
|
L

A
|
A

I

I

F
|
F

G

G

N

N

D

D

Y

Y

P

P

T

T

E

E

D

D

G

G

T

T

G

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

M

M

D

D

L

L

A

A

D

D

G

G

H

H

V

V

A

A

M

M

E

E

K

K

L

L

A

A

N

N

K

K

P

P

G

G

V

V

H

H

I

I

Y

Y

N

N

L

L

G

G

A

A

G

G

V

V

G

G

N

N

S

S

V
|
V

L

L

D

D

V

V

N

N

A

A

F

F

S

S

K

K

A

A

C

C

G

G

K

K

P

P

V

V

N

N

Y

Y

H

H

F

F

A

A

P

P

R

R

R
|
R

E

E

G

G

D
|
D

L

L

P

P

A

A

Y

Y

W

W

A

A

D

D

A

A

S

S

K

K

A

A

D

D

R

R

E

E

L

L

N

N

W

W

R

R

V

V

T

T

R

R

T

T

L

L

D

D

E

E

M

M

A

A

Q

Q

D

D

T

T

W

W

H

H

W

W

Q

Q

S

S

R

R

H

H

P

P

Q

Q

G

G

Y

Y

P

P

D

D

active site: S124 (= S124), A125 (= A125), T126 (= T126), Y149 (= Y149), K153 (= K153), M189 (= M189)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (= Y11), I12 (= I12), D31 (= D31), N32 (= N32), L33 (= L33), C34 (= C34), N35 (= N35), S36 (= S36), D58 (= D58), I59 (= I59), F80 (= F80), A81 (= A81), G82 (= G82), K84 (= K84), Y149 (= Y149), K153 (= K153), Y177 (= Y177), P180 (= P180)
binding uridine-diphosphate-n-acetylglucosamine: V86 (= V86), S124 (= S124), F178 (= F178), N179 (= N179), L200 (= L200), L215 (= L215), A216 (= A216), F218 (= F218), R231 (= R231), Y233 (= Y233), V269 (= V269), R292 (= R292), D295 (= D295)

1hzjA Human udp-galactose 4-epimerase: accommodation of udp-n- acetylglucosamine within the active site (see paper)
52% identity, 99% coverage: 2:336/338 of query aligns to 3:343/345 of 1hzjA

query
sites
1hzjA

R

K

V

V

L

L

V

V

T

T

G
|
G

G

G

S

A

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

V

V

L

Q

E

L

L

Q

E

N

A

G

G

H

Y

D

L

V

P

I

V

L

I

D
|
D

N
|
N

L

F

C
x
H

N
|
N

S

A

K

F

R

R

-

G

-

S

-

L

-

P

S

E

V

S

L

L

P

R

V

R

I

V

E

Q

R

E

L

L

G

T

G

G

K

R

H

S

P

V

T

E

F

F

V

E

E

E

G

M

D
|
D

I
|
I

R

L

N

D

E

Q

A

G

L

A

M

L

T

Q

E

R

I

L

L

F

H

K

D

K

H

Y

A

S

I

F

D

M

T

A

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

Q

Q

K

K

P

P

L

L

E

D

Y

Y

D

R

N

V

N

N

V

L

N

T

G

G

T

T

L

I

R

Q

L

L

I

L

S

E

A

I

M

M

R

K

A

A

A

H

N

G

V

V

K

K

N

N

F

L

I

V

F

F

S
|
S

S

S

S
|
S

A
|
A

T
|
T

V

V

Y

Y

G

G

D

N

Q

P

P

Q

K

Y

I

L

P

P

Y

L

V

D

E

E

S

A

F

H

P

P

T

T

G

G

T

G

P

C

Q

T

S

N

P

P

Y
|
Y

G

G

K

K

S

S

K
|
K

L

F

M

F

V

I

E

E

Q

E

I

M

L

I

T

R

D

D

L

L

Q

C

K

Q

A

A

Q

D

P

K

D

T

W

W

S

N

I

V

A

V

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

T

G

G

A

A

H

H

P

A

S

S

G

G

D

C

M

I

G

G

E

E

D

D

P

P

Q

Q

G

G

I

I

P

P

N

N

N
|
N

L
|
L

M

M

P

P

Y

Y

I

V

A

S

Q

Q

V

V

A

A

V

I

G

G

R

R

D

E

S

A

L

L

A
x
N

I
x
V

F
|
F

G

G

N

N

D

D

Y

Y

P

D

T

T

E

E

D

D

G

G

T

T

G

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

M

V

D

D

L

L

A

A

D

K

G

G

H

H

V

I

V

A

A

A

M

L

E

R

K

K

L

L

A

K

N

E

K

Q

P

C

G

G

V

C

H

R

I

I

Y

Y

N

N

L

L

G

G

A

T

G

G

V

T

G

G

N

Y

S

S

V
|
V

L

L

D

Q

V

M

V

V

N

Q

A

A

F

M

S

E

K

K

A

A

C

S

G

G

K

K

P

K

V

I

N

P

Y

Y

H

K

F

V

A

V

P

A

R

R

R
|
R

E

E

G

G

D
|
D

L

V

P

A

A

A

Y

C

W

Y

A

A

D

N

A

P

S

S

K

L

A

A

D

Q

R

E

E

E

L

L

N

G

W

W

R

T

V

A

T

A

R

L

T

G

L

L

D

D

E

R

M

M

A

C

Q

E

D

D

T

L

W

W

H

R

W

W

Q

Q

S

K

R

Q

H

N

P

P

Q

S

G

G

Y

F

active site: S131 (= S124), A132 (= A125), T133 (= T126), Y156 (= Y149), K160 (= K153)
binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), Y12 (= Y11), I13 (= I12), D32 (= D31), N33 (= N32), H35 (≠ C34), N36 (= N35), D65 (= D58), I66 (= I59), F87 (= F80), A88 (= A81), G89 (= G82), K91 (= K84), S129 (= S122), S131 (= S124), Y156 (= Y149), K160 (= K153), Y184 (= Y177), P187 (= P180)
binding uridine-diphosphate-n-acetylglucosamine: N186 (= N179), N206 (= N199), L207 (= L200), N223 (≠ A216), V224 (≠ I217), F225 (= F218), R238 (= R231), Y240 (= Y233), V276 (= V269), R299 (= R292), D302 (= D295)

1i3lA Molecular basis for severe epimerase-deficiency galactosemia: x-ray structure of the human v94m-substituted udp-galactose 4-epimerase (see paper)
52% identity, 99% coverage: 2:336/338 of query aligns to 3:343/347 of 1i3lA

query
sites
1i3lA

R

K

V

V

L

L

V

V

T

T

G
|
G

G

G

S

A

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

V

V

L

Q

E

L

L

Q

E

N

A

G

G

H

Y

D

L

V

P

I

V

L

I

D
|
D

N
|
N

L

F

C
x
H

N
|
N

S

A

K

F

R

R

-

G

-

S

-

L

-

P

S

E

V

S

L

L

P

R

V

R

I

V

E

Q

R

E

L

L

G

T

G

G

K

R

H

S

P

V

T

E

F

F

V

E

E

E

G

M

D
|
D

I
|
I

R

L

N

D

E

Q

A

G

L

A

M

L

T

Q

E

R

I

L

L

F

H

K

D

K

H

Y

A

S

I

F

D

M

T

A

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L

L

K
|
K

A

A

V

M

G

G

E

E

S

S

V

V

Q

Q

K

K

P

P

L

L

E

D

Y

Y

D

R

N

V

N

N

V

L

N

T

G

G

T

T

L

I

R

Q

L

L

I

L

S

E

A

I

M

M

R

K

A

A

A

H

N

G

V

V

K

K

N

N

F

L

I

V

F

F

S
|
S

A
|
A

T
|
T

V

V

Y

Y

G

G

D

N

Q

P

P

Q

K

Y

I

L

P

P

Y

L

V

D

E

E

S

A

F

H

P

P

T

T

G

G

T

G

P

C

Q

T

S

N

P

P

Y
|
Y

G

G

K

K

S

S

K
|
K

L

F

M

F

V

I

E

E

Q

E

I

M

L

I

T

R

D

D

L

L

Q

C

K

Q

A

A

Q

D

P

K

D

T

W

W

S

N

I

V

A

V

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

T

G

G

A

A

H

H

P

A

S

S

G

G

D

C

M

I

G

G

E

E

D

D

P

P

Q

Q

G

G

I

I

P

P

N

N

N
|
N

L
|
L

M

M

P

P

Y

Y

I

V

A

S

Q

Q

V

V

A

A

V

I

G

G

R

R

D

E

S

A

L

L

A
x
N

I
x
V

F
|
F

G

G

N

N

D

D

Y

Y

P

D

T

T

E

E

D

D

G

G

T

T

G

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

M

V

D

D

L

L

A

A

D

K

G

G

H

H

V

I

V

A

A

A

M

L

E

R

K

K

L

L

A

K

N

E

K

Q

P

C

G

G

V

C

H

R

I

I

Y

Y

N

N

L

L

G

G

A

T

G

G

V

T

G

G

N

Y

S

S

V
|
V

L

L

D

Q

V

M

V

V

N

Q

A

A

F

M

S

E

K

K

A

A

C

S

G

G

K

K

P

K

V

I

N

P

Y

Y

H

K

F

V

A

V

P

A

R

R

R
|
R

E

E

G

G

D
|
D

L

V

P

A

A

A

Y

C

W

Y

A

A

D

N

A

P

S

S

K

L

A

A

D

Q

R

E

E

E

L

L

N

G

W

W

R

T

V

A

T

A

R

L

T

G

L

L

D

D

E

R

M

M

A

C

Q

E

D

D

T

L

W

W

H

R

W

W

Q

Q

S

K

R

Q

H

N

P

P

Q

S

G

G

Y

F

active site: S131 (= S124), A132 (= A125), T133 (= T126), Y156 (= Y149), K160 (= K153)
binding galactose-uridine-5'-diphosphate: N186 (= N179), N206 (= N199), L207 (= L200), N223 (≠ A216), V224 (≠ I217), F225 (= F218), R238 (= R231), Y240 (= Y233), V276 (= V269), R299 (= R292), D302 (= D295)
binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), Y12 (= Y11), I13 (= I12), D32 (= D31), N33 (= N32), H35 (≠ C34), N36 (= N35), D65 (= D58), I66 (= I59), F87 (= F80), A88 (= A81), G89 (= G82), K91 (= K84), S129 (= S122), S130 (= S123), S131 (= S124), Y156 (= Y149), K160 (= K153), Y184 (= Y177), P187 (= P180)

1i3kA Molecular basis for severe epimerase-deficiency galactosemia: x-ray structure of the human v94m-substituted udp-galactose 4-epimerase (see paper)
52% identity, 99% coverage: 2:336/338 of query aligns to 3:343/347 of 1i3kA

query
sites
1i3kA

R

K

V

V

L

L

V

V

T

T

G
|
G

G

G

S

A

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

V

V

L

Q

E

L

L

Q

E

N

A

G

G

H

Y

D

L

V

P

I

V

L

I

D
|
D

N
|
N

L

F

C
x
H

N
|
N

S

A

K

F

R

R

-

G

-

S

-

L

-

P

S

E

V

S

L

L

P

R

V

R

I

V

E

Q

R

E

L

L

G

T

G

G

K

R

H

S

P

V

T

E

F

F

V

E

E

E

G

M

D
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D

I
|
I

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N

D

E

Q

A

G

L

A

M

L

T

Q

E

R

I

L

L

F

H

K

D

K

H

Y

A

S

I

F

D

M

T

A

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L

L

K
|
K

A

A

V

M

G

G

E

E

S

S

V

V

Q

Q

K

K

P

P

L

L

E

D

Y

Y

D

R

N

V

N

N

V

L

N

T

G

G

T

T

L

I

R

Q

L

L

I

L

S

E

A

I

M

M

R

K

A

A

A

H

N

G

V

V

K

K

N

N

F

L

I

V

F

F

S
|
S

S

S

S
|
S

A
|
A

T
|
T

V

V

Y

Y

G

G

D

N

Q

P

P

Q

K

Y

I

L

P

P

Y

L

V

D

E

E

S

A

F

H

P

P

T

T

G

G

T

G

P

C

Q

T

S

N

P

P

Y
|
Y

G

G

K

K

S

S

K
|
K

L

F

M

F

V

I

E

E

Q

E

I

M

L

I

T

R

D

D

L

L

Q

C

K

Q

A

A

Q

D

P

K

D

T

W

W

S

N

I

V

A

V

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

V

T

G

G

A

A

H

H

P

A

S

S

G

G

D

C

M

I

G

G

E

E

D

D

P

P

Q

Q

G

G

I

I

P

P

N

N

N
|
N

L
|
L

M

M

P

P

Y

Y

I

V

A

S

Q

Q

V

V

A

A

V

I

G

G

R

R

D

E

S

A

L

L

A
x
N

I
x
V

F
|
F

G

G

N

N

D

D

Y

Y

P

D

T

T

E

E

D

D

G

G

T

T

G

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

M

V

D

D

L

L

A

A

D

K

G

G

H

H

V

I

V

A

A

A

M

L

E

R

K

K

L

L

A

K

N

E

K

Q

P

C

G

G

V

C

H

R

I

I

Y

Y

N

N

L

L

G

G

A

T

G

G

V

T

G

G

N

Y

S

S

V
|
V

L

L

D

Q

V

M

V

V

N

Q

A

A

F

M

S

E

K

K

A

A

C

S

G

G

K

K

P

K

V

I

N

P

Y

Y

H

K

F

V

A

V

P

A

R

R

R
|
R

E

E

G

G

D
|
D

L

V

P

A

A

A

Y

C

W

Y

A

A

D

N

A

P

S

S

K

L

A

A

D

Q

R

E

E

E

L

L

N

G

W

W

R

T

V

A

T

A

R

L

T

G

L

L

D

D

E

R

M

M

A

C

Q

E

D

D

T

L

W

W

H

R

W

W

Q

Q

S

K

R

Q

H

N

P

P

Q

S

G

G

Y

F

active site: S131 (= S124), A132 (= A125), T133 (= T126), Y156 (= Y149), K160 (= K153)
binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), Y12 (= Y11), I13 (= I12), D32 (= D31), N33 (= N32), H35 (≠ C34), N36 (= N35), D65 (= D58), I66 (= I59), F87 (= F80), A88 (= A81), G89 (= G82), K91 (= K84), S129 (= S122), S131 (= S124), Y156 (= Y149), K160 (= K153), Y184 (= Y177), P187 (= P180)
binding uridine-5'-diphosphate-glucose: F185 (= F178), N186 (= N179), N206 (= N199), L207 (= L200), N223 (≠ A216), V224 (≠ I217), F225 (= F218), R238 (= R231), Y240 (= Y233), V276 (= V269), R299 (= R292), D302 (= D295)

Q14376 UDP-glucose 4-epimerase; Galactowaldenase; UDP-N-acetylgalactosamine 4-epimerase; UDP-GalNAc 4-epimerase; UDP-N-acetylglucosamine 4-epimerase; UDP-GlcNAc 4-epimerase; UDP-galactose 4-epimerase; EC 5.1.3.2; EC 5.1.3.7 from Homo sapiens (Human) (see 13 papers)
52% identity, 99% coverage: 2:336/338 of query aligns to 4:344/348 of Q14376

query
sites
Q14376

R

K

V

V

L

L

V

V

T

T

G

G

S

A

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

V

V

L

Q

E

L

L

Q

E

N

A

G

G

H

Y

D

L

V

P

I

V

L

I

D
|
D

N
|
N

L
x
F

C
x
H

N
|
N

S

A

K

F

R

R

-

G

-

S

-

L

-

P

S

E

V

S

L

L

P

R

V

R

I

V

E

Q

R

E

L

L

G

T

G

G

K

R

H

S

P

V

T

E

F

F

V

E

E

E

G

M

D
|
D

I
|
I

R

L

N

D

E

Q

A

G

L

A

M

L

T

Q

E

R

I

L

L

F

H

K

D

K

H

Y

A

S

I

F

D

M

T

A

V

V

I

I

H

H

F
|
F

A

A

G
|
G

L

L

K
|
K

A

A

V
|
V

G

G

E

E

S

S

V

V

Q

Q

K

K

P

P

L

L

E
x
D

Y

Y

D

R

N

V

N

N

V

L

N

T

G

G

T

T

L

I

R

Q

L

L

I

L

S

E

A

I

M

M

R

K

A

A

A

H

N

G

V

V

K

K

N

N

F

L

I

V

F

F

S

S

S
|
S

A
|
A

T
|
T

V

V

Y

Y

G

G

D

N

Q

P

P

Q

K

Y

I

L

P

P

Y

L

V

D

E

E

S

A

F

H

P

P

T

T

G

G

T

G

P

C

Q

T

S

N

P

P

Y
|
Y

G

G

K

K

S

S

K
|
K

L

F

M

F

V

I

E

E

Q

E

I

M

L

I

T

R

D

D

L

L

Q

C

K

Q

A

A

Q

D

P

K

D

T

W

W

S

N

I
x
A

A

V

L

L

L
|
L

R

R

Y
|
Y

F
|
F

N
|
N

P

P

V

T

G

G

A

A

H

H

P

A

S

S

G

G

D

C

M

I

G

G

E

E

D

D

P

P

Q

Q

G

G

I

I

P

P

N
|
N

L
|
L

M

M

P

P

Y

Y

I

V

A

S

Q

Q

V

V

A

A

V

I

G

G

R

R

D

E

S

A

L

L

A
x
N

I
x
V

F
|
F

G

G

N

N

D

D

Y

Y

P

D

T

T

E

E

D

D

G

G

T

T

G

G

V

V

R
|
R

D

D

Y

Y

I

I

H

H

V

V

M

V

D

D

L

L

A

A

D

K

G

G

H

H

V

I

V

A

A

A

M

L

E

R

K
|
K

L

L

A

K

N

E

K

Q

P

C

G

G

V

C

H

R

I

I

Y

Y

N

N

L

L

G

G

A

T

G

G

V

T

G

G

N

Y

S

S

V

V

L

L

D

Q

V

M

V

V

N

Q

A

A

F

M

S

E

K

K

A

A

C

S

G

G

K

K

P

K

V

I

N

P

Y

Y

H

K

F

V

A

V

P

A

R

R

R
|
R

E
|
E

G
|
G

D
|
D

L

V

P

A

A

A

Y
x
C

W

Y

A

A

D

N

A

P

S

S

K
x
L

A

A

D

Q

R

E

E

E

L

L

N
x
G

W

W

R

T

V

A

T

A

R

L

T

G

L

L

D

D

E

R

M

M

A

C

Q

E

D

D

T

L

W

W

H
x
R

W

W

Q

Q

S

K

R

Q

H

N

P

P

Q

S

G

G

Y

F

GYI 12:14 (= GYI 10:12) binding
DNFHN 33:37 (≠ DNLCN 31:35) binding
N34 (= N32) to S: in GALAC3; peripheral; nearly normal activity towards UDP-galactose; dbSNP:rs121908046
DI 66:67 (= DI 58:59) binding
F88 (= F80) binding
G90 (= G82) to E: in GALAC3; 800-fold decrease in UDP-galactose epimerization activity; dbSNP:rs28940882
K92 (= K84) binding
V94 (= V86) to M: in GALAC3; generalized; 30-fold decrease in UDP-galactose epimerization activity; 2-fold decrease in affinity for UDP-galactose; 24% of normal activity with respect to UDP-N-acetylgalactosamine; dbSNP:rs121908047
D103 (≠ E95) to G: in GALAC3; 7-fold decrease in UDP-galactose epimerization activity; very mild decrease in activity towards UDP-N-acetylgalactosamine; dbSNP:rs28940883
S132 (= S124) mutation to A: Loss of activity.
SAT 132:134 (= SAT 124:126) binding
Y157 (= Y149) active site, Proton acceptor; mutation to F: Loss of activity.
K161 (= K153) binding
A180 (≠ I172) to V: in dbSNP:rs3204468
L183 (= L175) to P: in GALAC3; peripheral; 3-fold decrease in UDP-galactose epimerization activity; dbSNP:rs121908045
Y185 (= Y177) binding
YFN 185:187 (= YFN 177:179) binding
NNL 206:208 (= NNL 198:200) binding
NVF 224:226 (≠ AIF 216:218) binding
R239 (= R231) binding
K257 (= K249) to R: in GALAC3; 7-fold decrease in UDP-galactose epimerization activity; does not affect affinity for UDP-galactose; dbSNP:rs28940884
REGD 300:303 (= REGD 292:295) binding
C307 (≠ Y299) mutation to Y: No effect on activity towards UDP-galactose. Loss of activity towards UDP-N-acetylgalactosamine.
L313 (≠ K305) to M: in GALAC3; 6-fold decrease in UDP-galactose epimerization activity; very mild decrease in activity towards UDP-N-acetylgalactosamine; dbSNP:rs3180383
G319 (≠ N311) to E: in GALAC3; nearly normal activity towards UDP-galactose; mild impairment under conditions of substrate limitation; dbSNP:rs28940885
R335 (≠ H327) to H: in GALAC3; 2-fold decrease in UDP-galactose epimerization activity; dbSNP:rs368637540

1ek6A Structure of human udp-galactose 4-epimerase complexed with nadh and udp-glucose (see paper)
52% identity, 99% coverage: 2:336/338 of query aligns to 4:344/346 of 1ek6A

query
sites
1ek6A

R

K

V

V

L

L

V

V

T

T

G
|
G

G

G

S

A

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

V

V

L

Q

E

L

L

Q

E

N

A

G

G

H

Y

D

L

V

P

I

V

L

I

D
|
D

N
|
N

L

F

C
x
H

N
|
N

S

A

K

F

R

R

-

G

-

S

-

L

-

P

S

E

V

S

L

L

P

R

V

R

I

V

E

Q

R

E

L

L

G

T

G

G

K

R

H

S

P

V

T

E

F

F

V

E

E

E

G

M

D
|
D

I
|
I

R

L

N

D

E

Q

A

G

L

A

M

L

T

Q

E

R

I

L

L

F

H

K

D

K

H

Y

A

S

I

F

D

M

T

A

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

Q

Q

K

K

P

P

L

L

E

D

Y

Y

D

R

N

V

N

N

V

L

N

T

G

G

T

T

L

I

R

Q

L

L

I

L

S

E

A

I

M

M

R

K

A

A

A

H

N

G

V

V

K

K

N

N

F

L

I

V

F

F

S
|
S

A
|
A

T
|
T

V

V

Y

Y

G

G

D

N

Q

P

P

Q

K

Y

I

L

P

P

Y

L

V

D

E

E

S

A

F

H

P

P

T

T

G

G

T

G

P

C

Q

T

S

N

P

P

Y
|
Y

G

G

K

K

S

S

K
|
K

L

F

M

F

V

I

E

E

Q

E

I

M

L

I

T

R

D

D

L

L

Q

C

K

Q

A

A

Q

D

P

K

D

T

W

W

S

N

I

A

A

V

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

V

T

G

G

A

A

H

H

P

A

S

S

G

G

D

C

M

I

G

G

E

E

D

D

P

P

Q

Q

G

G

I

I

P

P

N

N

N
|
N

L
|
L

M

M

P

P

Y

Y

I

V

A

S

Q

Q

V

V

A

A

V

I

G

G

R

R

D

E

S

A

L

L

A
x
N

I
x
V

F
|
F

G

G

N

N

D

D

Y

Y

P

D

T

T

E

E

D

D

G

G

T

T

G

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

M

V

D

D

L

L

A

A

D

K

G

G

H

H

V

I

V

A

A

A

M

L

E

R

K

K

L

L

A

K

N

E

K

Q

P

C

G

G

V

C

H

R

I

I

Y

Y

N

N

L

L

G

G

A

T

G

G

V

T

G

G

N

Y

S

S

V
|
V

L

L

D

Q

V

M

V

V

N

Q

A

A

F

M

S

E

K

K

A

A

C

S

G

G

K

K

P

K

V

I

N

P

Y

Y

H

K

F

V

A

V

P

A

R

R

R
|
R

E

E

G

G

D
|
D

L

V

P

A

A

A

Y

C

W

Y

A

A

D

N

A

P

S

S

K

L

A

A

D

Q

R

E

E

E

L

L

N

G

W

W

R

T

V

A

T

A

R

L

T

G

L

L

D

D

E

R

M

M

A

C

Q

E

D

D

T

L

W

W

H

R

W

W

Q

Q

S

K

R

Q

H

N

P

P

Q

S

G

G

Y

F

active site: S132 (= S124), A133 (= A125), T134 (= T126), Y157 (= Y149), K161 (= K153)
binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G7), G12 (= G10), Y13 (= Y11), I14 (= I12), D33 (= D31), N34 (= N32), H36 (≠ C34), N37 (= N35), D66 (= D58), I67 (= I59), F88 (= F80), A89 (= A81), G90 (= G82), K92 (= K84), S130 (= S122), S131 (= S123), S132 (= S124), Y157 (= Y149), K161 (= K153), Y185 (= Y177), P188 (= P180)
binding uridine-5'-diphosphate-glucose: S132 (= S124), Y157 (= Y149), F186 (= F178), N187 (= N179), N207 (= N199), L208 (= L200), N224 (≠ A216), V225 (≠ I217), F226 (= F218), R239 (= R231), Y241 (= Y233), V277 (= V269), R300 (= R292), D303 (= D295)

6k0iA Crystal structure of udp-glucose 4-epimerase from bifidobacterium longum in complex with NAD+ and udp-glc (see paper)
55% identity, 99% coverage: 3:336/338 of query aligns to 3:335/335 of 6k0iA

query
sites
6k0iA

V

V

L

L

V

V

T

T

G
|
G

G

G

S

A

G
|
G

Y
x
F

I
|
I

G

A

S

T

H

H

T

T

C

D

V

I

Q

E

L

L

Q

N

N

K

G

G

H

Y

D

D

V

V

I

I

I

S

L

V

D
|
D

N
|
N

L

Y

C

G

N
|
N

S
|
S

K

S

R

P

S

V

V

A

L

L

P

E

V

R

I

V

E

E

R

Q

L

I

G

T

G

G

K

K

H

P

P

V

T

K

F

R

V

Y

E

D

G

G

D
|
D

I
x
V

R

R

N

D

E

E

A

A

L

L

M

M

T

E

E

R

I

V

L

F

H

A

D

E

H

N

A

N

I

I

D

D

T

W

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

Q

A

K

K

P

P

L

I

E

E

Y

Y

D

D

N
|
N

N

N

V

L

N

Y

G

S

T

T

L

L

R

V

L

L

I

L

S

K

A

V

M

M

R

K

A

K

A

H

N

N

V

V

K

K

N

K

F

I

I

I

F

F

S
|
S

A
|
A

T
|
T

V

V

Y

Y

G

G

D

T

Q

P

P

K

K

E

I

L

P

P

Y

I

V

T

E

E

S

E

F

T

P

P

T

T

G

G

T

G

P

T

Q

T

S

N

P

P

Y
|
Y

G

G

K

T

S

S

K
|
K

L

L

M

F

V

Q

E

E

Q

Q

I

I

L

L

T

R

D

D

L

V

Q

H

K

V

A

A

Q

D

P

P

D

S

W

W

S

T

I

I

A

V

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

E

S

S

G

G

D

L

M

L

G

G

E

E

D

D

P

P

Q

K

G

G

I

I

P

P

N
x
A

N
|
N

L
|
L

M

T

P

P

Y

Y

I

V

A

A

Q

K

V

V

A

A

V

V

G

G

R

E

R

L

D

K

S

E

L

V

A
x
Q

I
x
V

F
x
Y

G

G

N

D

D

D

Y

Y

P

D

T

T

E

P

D

D

G

G

T

T

G

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

M

V

D

D

L

L

A

A

D

K

G

G

H

H

V

V

V

A

A

V

M

I

E

D

K

H

L

I

A

-

N

D

K

K

P

E

G

G

V

V

H

F

I

V

Y

Y

N

N

L

L

G

G

A

T

G

G

V

H

G

G

N

Y

S

S

V
|
V

L

L

D

E

V

V

V

I

N

K

A

A

F

Y

S

E

K

K

A

A

C

A

G

G

K

H

P

P

V

I

N

P

Y

Y

H

A

F

I

A

K

P

P

R

R

R
|
R

E

P

G

G

D
|
D

L

I

P

A

A

A

Y

C

W

Y

A

A

D

D

A

A

S

S

K

K

A

A

D

E

R

K

E

E

L

L

N

G

W

W

R

K

V

A

T

E

R

L

T

T

L

I

D

D

E

D

M

M

A

A

Q

A

D

S

T

S

W

L

H

N

W

W

Q

Q

S

T

R

K

H

N

P

P

Q

N

G

G

Y

F

active site: S124 (= S124), A125 (= A125), T126 (= T126), Y149 (= Y149), K153 (= K153)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (≠ Y11), I12 (= I12), D31 (= D31), N32 (= N32), N35 (= N35), S36 (= S36), D58 (= D58), V59 (≠ I59), F80 (= F80), A81 (= A81), G82 (= G82), K84 (= K84), N99 (= N99), S122 (= S122), S123 (= S123), Y149 (= Y149), K153 (= K153), Y177 (= Y177), P180 (= P180)
binding uridine-5'-diphosphate-glucose: K84 (= K84), S124 (= S124), Y149 (= Y149), F178 (= F178), N179 (= N179), A198 (≠ N198), N199 (= N199), L200 (= L200), Q216 (≠ A216), V217 (≠ I217), Y218 (≠ F218), R231 (= R231), Y233 (= Y233), V268 (= V269), R291 (= R292), D294 (= D295)

6k0hA Crystal structure of udp-glucose 4-epimerase from bifidobacterium longum in complex with NAD+ and udp-glcnac (see paper)
55% identity, 99% coverage: 3:336/338 of query aligns to 3:335/335 of 6k0hA

query
sites
6k0hA

V

V

L

L

V

V

T

T

G
|
G

G

G

S

A

G
|
G

Y
x
F

I
|
I

G

A

S

T

H

H

T

T

C

D

V

I

Q

E

L

L

Q

N

N

K

G

G

H

Y

D

D

V

V

I

I

I

S

L

V

D
|
D

N
|
N

L

Y

C
x
G

N
|
N

S
|
S

K

S

R

P

S

V

V

A

L

L

P

E

V

R

I

V

E

E

R

Q

L

I

G

T

G

G

K

K

H

P

P

V

T

K

F

R

V

Y

E

D

G

G

D
|
D

I
x
V

R

R

N

D

E

E

A

A

L

L

M

M

T

E

E

R

I

V

L

F

H

A

D

E

H

N

A

N

I

I

D

D

T

W

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

Q

A

K

K

P

P

L

I

E

E

Y

Y

D

D

N
|
N

N

N

V

L

N

Y

G

S

T

T

L

L

R

V

L

L

I

L

S

K

A

V

M

M

R

K

A

K

A

H

N

N

V

V

K

K

N

K

F

I

I

I

F

F

S

S

S
|
S

A
|
A

T
|
T

V

V

Y

Y

G

G

D

T

Q

P

P

K

K

E

I

L

P

P

Y

I

V

T

E

E

S

E

F

T

P

P

T

T

G

G

T

G

P

T

Q

T

S

N

P

P

Y
|
Y

G

G

K

T

S

S

K
|
K

L

L

M

F

V

Q

E

E

Q

Q

I

I

L

L

T

R

D

D

L

V

Q

H

K

V

A

A

Q

D

P

P

D

S

W

W

S

T

I

I

A

V

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

E

S

S

G

G

D

L

M

L

G

G

E

E

D

D

P

P

Q

K

G

G

I

I

P

P

N
x
A

N
|
N

L
|
L

M

T

P

P

Y

Y

I

V

A

A

Q

K

V

V

A

A

V

V

G

G

R

E

R

L

D

K

S

E

L

V

A
x
Q

I
x
V

F
x
Y

G

G

N

D

D

D

Y

Y

P

D

T

T

E

P

D

D

G

G

T

T

G

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

M

V

D

D

L

L

A

A

D

K

G

G

H

H

V

V

V

A

A

V

M

I

E

D

K

H

L

I

A

-

N

D

K

K

P

E

G

G

V

V

H

F

I

V

Y

Y

N

N

L

L

G

G

A

T

G

G

V

H

G

G

N

Y

S

S

V
|
V

L

L

D

E

V

V

V

I

N

K

A

A

F

Y

S

E

K

K

A

A

C

A

G

G

K

H

P

P

V

I

N

P

Y

Y

H

A

F

I

A

K

P

P

R

R

R
|
R

E

P

G

G

D
|
D

L

I

P

A

A

A

Y

C

W

Y

A

A

D

D

A

A

S

S

K

K

A

A

D

E

R

K

E

E

L

L

N

G

W

W

R

K

V

A

T

E

R

L

T

T

L

I

D

D

E

D

M

M

A

A

Q

A

D

S

T

S

W

L

H

N

W

W

Q

Q

S

T

R

K

H

N

P

P

Q

N

G

G

Y

F

active site: S124 (= S124), A125 (= A125), T126 (= T126), Y149 (= Y149), K153 (= K153)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (≠ Y11), I12 (= I12), D31 (= D31), N32 (= N32), G34 (≠ C34), N35 (= N35), S36 (= S36), D58 (= D58), V59 (≠ I59), F80 (= F80), A81 (= A81), G82 (= G82), K84 (= K84), N99 (= N99), S123 (= S123), Y149 (= Y149), K153 (= K153), Y177 (= Y177), P180 (= P180)
binding uridine-diphosphate-n-acetylglucosamine: K84 (= K84), S124 (= S124), Y149 (= Y149), F178 (= F178), N179 (= N179), A198 (≠ N198), N199 (= N199), L200 (= L200), Q216 (≠ A216), V217 (≠ I217), Y218 (≠ F218), R231 (= R231), Y233 (= Y233), V268 (= V269), R291 (= R292), D294 (= D295)

6k0gA Crystal structure of udp-glucose 4-epimerase from bifidobacterium longum in complex with NAD+ and udp (see paper)
55% identity, 99% coverage: 3:336/338 of query aligns to 3:335/335 of 6k0gA

query
sites
6k0gA

V

V

L

L

V

V

T

T

G
|
G

G

G

S

A

G
|
G

Y
x
F

I
|
I

G

A

S

T

H

H

T

T

C

D

V

I

Q

E

L

L

Q

N

N

K

G

G

H

Y

D

D

V

V

I

I

I

S

L

V

D
|
D

N
|
N

L

Y

C

G

N
|
N

S
|
S

K

S

R

P

S

V

V

A

L

L

P

E

V

R

I

V

E

E

R

Q

L

I

G

T

G

G

K

K

H

P

P

V

T

K

F

R

V

Y

E

D

G

G

D
|
D

I
x
V

R

R

N

D

E

E

A

A

L

L

M

M

T
x
E

E

R

I

V

L

F

H

A

D

E

H

N

A

N

I

I

D

D

T

W

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

Q

A

K

K

P

P

L

I

E

E

Y

Y

D

D

N
|
N

N

N

V

L

N

Y

G

S

T

T

L

L

R

V

L

L

I

L

S

K

A

V

M

M

R

K

A

K

A
x
H

N

N

V

V

K

K

N

K

F

I

I

I

F

F

S
|
S

A
|
A

T
|
T

V

V

Y

Y

G

G

D

T

Q

P

P

K

K

E

I

L

P

P

Y

I

V

T

E

E

S

E

F

T

P

P

T

T

G

G

T

G

P

T

Q

T

S

N

P

P

Y
|
Y

G

G

K

T

S

S

K
|
K

L

L

M

F

V

Q

E

E

Q

Q

I

I

L

L

T

R

D

D

L

V

Q

H

K

V

A

A

Q

D

P

P

D

S

W

W

S

T

I

I

A

V

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

E

S

S

G

G

D

L

M

L

G

G

E

E

D

D

P

P

Q

K

G

G

I

I

P

P

N
x
A

N
|
N

L
|
L

M

T

P

P

Y

Y

I

V

A

A

Q

K

V

V

A

A

V

V

G

G

R

E

R

L

D

K

S

E

L

V

A
x
Q

I
x
V

F
x
Y

G

G

N

D

D

D

Y

Y

P

D

T

T

E

P

D

D

G

G

T

T

G

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

M

V

D

D

L

L

A

A

D

K

G

G

H

H

V

V

V

A

A

V

M

I

E

D

K

H

L

I

A

-

N

D

K

K

P

E

G

G

V

V

H

F

I

V

Y

Y

N

N

L

L

G

G

A

T

G

G

V

H

G

G

N

Y

S

S

V
|
V

L

L

D

E

V

V

V

I

N

K

A

A

F

Y

S

E

K

K

A

A

C

A

G

G

K

H

P

P

V

I

N

P

Y

Y

H

A

F

I

A

K

P

P

R

R

R
|
R

E

P

G

G

D
|
D

L

I

P

A

A

A

Y

C

W

Y

A

A

D

D

A

A

S

S

K

K

A

A

D

E

R

K

E

E

L

L

N

G

W

W

R

K

V

A

T

E

R

L

T

T

L

I

D

D

E

D

M

M

A

A

Q

A

D

S

T

S

W

L

H

N

W

W

Q

Q

S

T

R

K

H

N

P

P

Q

N

G

G

Y

F

active site: S124 (= S124), A125 (= A125), T126 (= T126), Y149 (= Y149), K153 (= K153)
binding magnesium ion: E66 (≠ T66), H114 (≠ A114)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (≠ Y11), I12 (= I12), D31 (= D31), N32 (= N32), N35 (= N35), S36 (= S36), D58 (= D58), V59 (≠ I59), F80 (= F80), A81 (= A81), G82 (= G82), K84 (= K84), N99 (= N99), S122 (= S122), S123 (= S123), Y149 (= Y149), K153 (= K153), Y177 (= Y177), P180 (= P180)
binding uridine-5'-diphosphate: N179 (= N179), A198 (≠ N198), N199 (= N199), L200 (= L200), Q216 (≠ A216), V217 (≠ I217), Y218 (≠ F218), R231 (= R231), Y233 (= Y233), V268 (= V269), R291 (= R292), D294 (= D295)

7xpqA Crystal structure of udp-glc/glcnac 4-epimerase with NAD/udp-glcnac (see paper)
53% identity, 99% coverage: 3:336/338 of query aligns to 4:342/343 of 7xpqA

query
sites
7xpqA

V

I

L

L

V

V

T

T

G
|
G

G

G

S

A

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

V

V

L

Q

Q

L

L

Q

Q

N

Q

G

G

H

F

D

R

V

V

I

V

L

V

D
|
D

N
|
N

L

L

C

D

N
|
N

S

A

K

S

R

E

S

A

V

A

L

L

P

A

V

R

I

V

E

A

R

E

L

L

G

A

G

G

-

H

-

D

-

G

K

A

H

N

P

L

T

V

F

F

V

H

E

K

G

V

D
|
D

I
x
L

R

R

N

D

E

R

A

H

L

A

M

L

T

V

E

D

I

I

L

F

H

S

D

S

H

H

A

R

I

F

D

E

T

A

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

Q

H

K

K

P

P

L

L

E

L

Y

Y

D

D

N
|
N

N

N

V

L

N

V

G

G

T

T

L

I

R

T

L

L

I

L

S

E

A

V

M

M

R

A

A

A

A

N

N

G

V

C

K

K

N

K

F

L

I

V

F

F

S
|
S

S

S

S
|
S

A

A

T

T

V

V

Y

Y

G

G

D

W

Q

P

P

K

K

E

I

V

P

P

Y

C

V

T

E

E

S

E

F

F

P

P

T

L

G

-

T

C

P

A

Q

T

S

N

P

P

Y
|
Y

G

G

K

R

S

T

K
|
K

L

L

M

V

V

I

E

E

Q

D

I

I

L

C

T

R

D

D

L

V

Q

H

K

R

A

S

Q

D

P

P

D

D

W

W

S

K

I

I

A

I

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

P

S

S

G

G

D

Y

M

I

G

G

E

E

D

D

P

P

Q

C

G

G

I

V

P

P

N

N

N
|
N

L
|
L

M

M

P

P

Y

Y

I

V

A

Q

Q

Q

V

V

A

A

V

V

G

G

R

R

R

L

D

P

S

H

L

L

A
x
T

I

V

F
x
Y

G

G

N

T

D

D

Y

Y

P

S

T

T

E

K

D

D

G

G

T

T

G

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

M

V

D

D

L

L

A

A

D

D

G

G

H

H

V

I

V

A

A

A

M

L

E

R

K

K

L

L

-

Y

-

E

-

D

A

S

N

D

K

K

P

I

G

G

V

C

H

E

I

V

Y

Y

N

N

L

L

G

G

A

T

G

G

V

K

G

G

N

T

S

S

V
|
V

L

L

D

E

V

M

V

V

N

A

A

A

F

F

S

E

K

K

A

A

C

S

G

G

K

K

P

K

V

I

N

P

Y

L

H

V

F

F

A

A

P

G

R

R

R
|
R

E

P

G

G

D
|
D

L

A

P

E

A

I

Y

V

W

Y

A

A

D

A

A

T

S

A

K

K

A

A

D

E

R

K

E

E

L

L

N

K

W

W

R

K

V

A

T

K

R

Y

T

G

L

I

D

E

E

E

M

M

A

C

Q

R

D

D

T

L

W

W

H

N

W

W

Q

A

S

S

R

K

H

N

P

P

Q

Y

G

G

Y

Y

binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), Y12 (= Y11), I13 (= I12), D32 (= D31), N33 (= N32), N36 (= N35), D62 (= D58), L63 (≠ I59), F84 (= F80), A85 (= A81), G86 (= G82), K88 (= K84), N103 (= N99), S126 (= S122), S128 (= S124), Y152 (= Y149), K156 (= K153), Y180 (= Y177), P183 (= P180)
binding uridine-diphosphate-n-acetylglucosamine: N182 (= N179), N202 (= N199), L203 (= L200), T219 (≠ A216), Y221 (≠ F218), R234 (= R231), Y236 (= Y233), V275 (= V269), R298 (= R292), D301 (= D295)

7xpoA Crystal structure of udp-glc/glcnac 4-epimerase with NAD/udp-glc (see paper)
53% identity, 99% coverage: 3:336/338 of query aligns to 4:342/344 of 7xpoA

query
sites
7xpoA

V

I

L

L

V

V

T

T

G
|
G

G

G

S

A

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

V

V

L

Q

Q

L

L

Q

Q

N

Q

G

G

H

F

D

R

V

V

I

V

L

V

D
|
D

N
|
N

L

L

C

D

N
|
N

S

A

K

S

R

E

S

A

V

A

L

L

P

A

V

R

I

V

E

A

R

E

L

L

G

A

G

G

-

H

-

D

-

G

K

A

H

N

P

L

T

V

F

F

V

H

E

K

G

V

D
|
D

I
x
L

R

R

N

D

E

R

A

H

L

A

M

L

T

V

E

D

I

I

L

F

H

S

D

S

H

H

A

R

I

F

D

E

T

A

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

Q

H

K

K

P

P

L

L

E

L

Y

Y

D

D

N
|
N

N

N

V

L

N

V

G

G

T

T

L

I

R

T

L

L

I

L

S

E

A

V

M

M

R

A

A

A

A

N

N

G

V

C

K

K

N

K

F

L

I

V

F

F

S
|
S

S

S

S
|
S

A
|
A

T

T

V

V

Y

Y

G

G

D

W

Q

P

P

K

K

E

I

V

P

P

Y

C

V

T

E

E

S

E

F

F

P

P

T

L

G

-

T

C

P

A

Q

T

S

N

P

P

Y
|
Y

G

G

K

R

S

T

K
|
K

L

L

M

V

V

I

E

E

Q

D

I

I

L

C

T

R

D

D

L

V

Q

H

K

R

A

S

Q

D

P

P

D

D

W

W

S

K

I

I

A

I

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

P

S

S

G

G

D

Y

M

I

G

G

E

E

D

D

P

P

Q

C

G

G

I

V

P

P

N

N

N
|
N

L
|
L

M

M

P

P

Y

Y

I

V

A

Q

Q

Q

V

V

A

A

V

V

G

G

R

R

R

L

D

P

S

H

L

L

A
x
T

I
x
V

F
x
Y

G

G

N

T

D

D

Y

Y

P

S

T

T

E

K

D

D

G

G

T

T

G

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

M

V

D

D

L

L

A

A

D

D

G

G

H

H

V

I

V

A

A

A

M

L

E

R

K

K

L

L

-

Y

-

E

-

D

A

S

N

D

K

K

P

I

G

G

V

C

H

E

I

V

Y

Y

N

N

L

L

G

G

A

T

G

G

V

K

G

G

N

T

S

S

V
|
V

L

L

D

E

V

M

V

V

N

A

A

A

F

F

S

E

K

K

A

A

C

S

G

G

K

K

P

K

V

I

N

P

Y

L

H

V

F

F

A

A

P

G

R

R

R
|
R

E

P

G

G

D
|
D

L

A

P

E

A

I

Y

V

W

Y

A

A

D

A

A

T

S

A

K

K

A

A

D

E

R

K

E

E

L

L

N

K

W

W

R

K

V

A

T

K

R

Y

T

G

L

I

D

E

E

E

M

M

A

C

Q

R

D

D

T

L

W

W

H

N

W

W

Q

A

S

S

R

K

H

N

P

P

Q

Y

G

G

Y

Y

binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), Y12 (= Y11), I13 (= I12), D32 (= D31), N33 (= N32), N36 (= N35), D62 (= D58), L63 (≠ I59), F84 (= F80), A85 (= A81), G86 (= G82), K88 (= K84), N103 (= N99), S126 (= S122), S128 (= S124), Y152 (= Y149), K156 (= K153), Y180 (= Y177), P183 (= P180)
binding uridine-5'-diphosphate-glucose: S128 (= S124), A129 (= A125), F181 (= F178), N182 (= N179), N202 (= N199), L203 (= L200), T219 (≠ A216), V220 (≠ I217), Y221 (≠ F218), R234 (= R231), Y236 (= Y233), V275 (= V269), R298 (= R292), D301 (= D295)

3enkA 1.9a crystal structure of udp-glucose 4-epimerase from burkholderia pseudomallei
54% identity, 99% coverage: 3:336/338 of query aligns to 7:339/340 of 3enkA

query
sites
3enkA

V

I

L

L

V

V

T

T

G
|
G

G

G

S

A

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

A

V

V

Q

E

L

L

Q

A

N

H

G

G

H

Y

D

D

V

V

I

V

I

I

L

A

D
|
D

N
|
N

L

L

C
x
V

N
|
N

S
|
S

K

K

R

R

S

E

V

A

L

I

P

A

V

R

I

I

E

E

R

K

L

I

G

T

G

G

K

K

H

T

P

P

T

A

F

F

V

H

E

E

G

T

D
|
D

I
x
V

R

S

N

D

E

E

A

R

L

A

M

L

T

A

E

R

I

I

L

F

H

D

D

A

H

H

A

P

I

I

D

T

T

A

V

A

I

I

H

H

F
|
F

A
|
A

G
x
A

L

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

Q

A

K

K

P

P

L

I

E

E

Y

Y

D

R

N
|
N

N

N

V

L

N

D

G

S

T

L

L

L

R

S

L

L

I

L

S

R

A

V

M

M

R

R

A

E

A

R

N

A

V

V

K

K

N

R

F

I

I

V

F

F

S
|
S

A

A

T
|
T

V

V

Y

Y

G

G

D

V

Q

P

P

E

K

R

I

S

P

P

Y

I

V

D

E

E

S

T

F

F

P

P

T

L

G

-

T

S

P

A

Q

T

S

N

P

P

Y
|
Y

G

G

K

Q

S

T

K
|
K

L

L

M

M

V

A

E

E

Q

Q

I

I

L

L

T

R

D

D

L

V

Q

E

K

A

A

A

Q

D

P

P

D

S

W

W

S

R

I

V

A

A

L

T

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

E

S

S

G

G

D

L

M

I

G

G

E

E

D

D

P

P

Q

A

G

G

I

I

P

P

N
|
N

L
|
L

M

M

P

P

Y

Y

I

V

A

A

Q

Q

V

V

A

A

V

V

G

G

R

K

R

L

D

E

S

K

L

L

A
x
R

I
x
V

F
|
F

G

G

N

S

D

D

Y

Y

P

P

T

T

E

P

D

D

G

G

T

T

G

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

M

V

D

D

L

L

A

A

D

R

G

G

H

H

V

I

V

A

A

A

M

L

E

D

K

A

L

L

A

E

N

R

K

R

P

D

G

A

V

S

H

L

I

T

Y

V

N

N

L

L

G

G

A

T

G

G

V

R

G

G

N

Y

S

S

V
|
V

L

L

D

E

V

V

N

R

A

A

F

F

S

E

K

K

A

A

C

S

G

G

K

R

P

A

V

V

N

P

Y

Y

H

E

F

L

A

V

P

A

R

R

R
|
R

E

P

G

G

D
|
D

L

V

P

A

A

E

Y

C

W

Y

A

A

D

N

A

P

S

A

K

A

A

A

D

A

R

E

E

T

L

I

N

G

W

W

R

K

V

A

T

E

R

R

T

D

L

L

D

E

E

R

M

M

A

C

Q

A

D

D

T

H

W

W

H

R

W

W

Q

Q

S

E

R

N

H

N

P

P

Q

R

G

G

Y

F

active site: S127 (= S123), S128 (= S124), T130 (= T126), Y152 (= Y149), K156 (= K153)
binding nicotinamide-adenine-dinucleotide: G11 (= G7), G14 (= G10), Y15 (= Y11), I16 (= I12), D35 (= D31), N36 (= N32), V38 (≠ C34), N39 (= N35), S40 (= S36), D62 (= D58), V63 (≠ I59), F84 (= F80), A85 (= A81), A86 (≠ G82), K88 (= K84), N103 (= N99), S126 (= S122), S128 (= S124), Y152 (= Y149), K156 (= K153), Y180 (= Y177), P183 (= P180)
binding uridine-5'-diphosphate-glucose: T130 (= T126), N182 (= N179), N201 (= N198), N202 (= N199), L203 (= L200), R219 (≠ A216), V220 (≠ I217), F221 (= F218), R234 (= R231), Y236 (= Y233), V272 (= V269), R295 (= R292), D298 (= D295)

7kn1A Crystal structure of udp-glucose-4-epimerase (gale) from stenotrophomonas maltophila with bound NAD and formylated udp- arabinopyranose
53% identity, 97% coverage: 1:329/338 of query aligns to 3:330/336 of 7kn1A

query
sites
7kn1A

M

M

R

N

V

V

L

L

V

V

T

T

G
|
G

G

G

S

A

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

A

C

C

V

V

Q

E

L

L

L

Q

Q

Q

N

Q

G

G

H

H

D

G

V

V

I

V

I

I

L

V

D
|
D

N
x
S

L

L

C
|
C

N
|
N

S

S

K

D

R

A

S

S

V

V

L

V

P

E

V

R

I

I

E

G

R

R

L

I

G

T

G

G

K

T

H

A

P

P

T

V

F

F

V

V

E

Q

G

A

D
|
D

I
|
I

R

R

N

D

E

R

A

P

L

R

M

M

T

A

E

A

I

L

L

M

H

Q

D

E

H

H

A

A

I

I

D

D

T

A

V

V

I

L

H

H

F
|
F

A
|
A

G
x
A

L

L

K
|
K

A

S

V
|
V

G

G

E

E

S

S

V

Q

Q

K

K

I

P

P

L

L

E

Q

Y

Y

Y

F

D

D

N

S

N

N

V

I

N

S

G

G

T

S

L

I

R

A

L

L

I

L

S

G

A

A

M

M

R

Q

A

D

A

A

N

G

V

V

K

Q

N

L

F

L

I

V

F

F

S
|
S

A

A

T

T

V

V

Y

Y

G

G

D

N

Q

Q

P

D

K

H

I

C

P

P

Y

V

V

A

E

E

S

T

F

A

P

S

T

T

G

-

T

C

P

A

Q

M

S

T

P

P

Y
|
Y

G

G

K

R

S

T

K
|
K

L

L

M

V

V

V

E

E

Q

Q

I

L

L

L

T

A

D

D

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active site: S126 (= S124), Y150 (= Y149), K154 (= K153)
binding nicotinamide-adenine-dinucleotide: G9 (= G7), G12 (= G10), Y13 (= Y11), I14 (= I12), D33 (= D31), S34 (≠ N32), C36 (= C34), N37 (= N35), D60 (= D58), I61 (= I59), F82 (= F80), A83 (= A81), A84 (≠ G82), K86 (= K84), S124 (= S122), S125 (= S123), S126 (= S124), Y150 (= Y149), K154 (= K153), Y178 (= Y177), P181 (= P180)
binding UDP-4-deoxy-4-formamido-beta-L-arabinopyranose: V88 (= V86), S126 (= S124), Y150 (= Y149), N180 (= N179), S199 (≠ N198), N200 (= N199), L201 (= L200), Q217 (≠ A216), V218 (≠ I217), F219 (= F218), R232 (= R231), Y234 (= Y233), V270 (= V269), R293 (= R292), D296 (= D295)

Query Sequence

>14884 b0759 UDP-galactose-4-epimerase (VIMSS)
MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVIERLGGKHPTFVEGDIR
NEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVNGTLRLISAMRAANVKNFI
FSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQILTDLQKAQPDWSIALLRYFNP
VGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIFGNDYPTEDGTGVRDYIHVMDLA
DGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAFSKACGKPVNYHFAPRREGDLPAYW
ADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQGYPD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory