SitesBLAST
Comparing 14980 b0855 ATP-binding component of putrescine transport system (VIMSS) to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
45% identity, 88% coverage: 11:342/377 of query aligns to 9:339/378 of P69874
- C26 (≠ S28) mutation to A: Lower ATPase activity and transport efficiency.
- F27 (≠ Y29) mutation to L: Lower ATPase activity and transport efficiency.
- F45 (≠ I47) mutation to L: Lower ATPase activity and transport efficiency.
- C54 (= C56) mutation to T: Loss of ATPase activity and transport.
- L60 (= L62) mutation to F: Lower ATPase activity and transport efficiency.
- L76 (= L78) mutation to P: Lower ATPase activity and transport efficiency.
- V135 (= V137) mutation to M: Loss of ATPase activity and transport.
- D172 (= D174) mutation to N: Loss of ATPase activity and transport.
- C276 (≠ L279) mutation to A: Lower ATPase activity and transport efficiency.
- E297 (= E300) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.
2d62A Crystal structure of multiple sugar binding transport atp- binding protein
51% identity, 61% coverage: 24:252/377 of query aligns to 11:245/375 of 2d62A
1g291 Malk (see paper)
48% identity, 65% coverage: 24:269/377 of query aligns to 8:258/372 of 1g291
- binding magnesium ion: D69 (≠ Q85), E71 (vs. gap), K72 (vs. gap), K79 (≠ Y89), D80 (≠ L90)
- binding pyrophosphate 2-: S38 (= S54), G39 (= G55), C40 (= C56), G41 (= G57), K42 (= K58), T43 (≠ S59), T44 (= T60)
Sites not aligning to the query:
8hprC Lpqy-sugabc in state 4 (see paper)
49% identity, 62% coverage: 25:256/377 of query aligns to 8:242/363 of 8hprC
- binding adenosine-5'-triphosphate: Y12 (= Y29), S38 (= S54), G39 (= G55), G41 (= G57), K42 (= K58), S43 (= S59), Q82 (= Q98), Q133 (= Q149), G136 (= G152), G137 (= G153), Q138 (= Q154), H192 (= H208)
- binding magnesium ion: S43 (= S59), Q82 (= Q98)
8hprD Lpqy-sugabc in state 4 (see paper)
49% identity, 62% coverage: 25:256/377 of query aligns to 8:242/362 of 8hprD
- binding adenosine-5'-triphosphate: Y12 (= Y29), S38 (= S54), C40 (= C56), G41 (= G57), K42 (= K58), S43 (= S59), T44 (= T60), Q82 (= Q98), R129 (= R145), Q133 (= Q149), S135 (= S151), G136 (= G152), G137 (= G153), Q159 (≠ E175), H192 (= H208)
- binding magnesium ion: S43 (= S59), Q82 (= Q98)
8hplC Lpqy-sugabc in state 1 (see paper)
49% identity, 62% coverage: 25:256/377 of query aligns to 8:240/384 of 8hplC
P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
50% identity, 62% coverage: 24:256/377 of query aligns to 8:243/393 of P9WQI3
- H193 (= H208) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.
1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
42% identity, 84% coverage: 20:336/377 of query aligns to 7:311/353 of 1vciA
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
43% identity, 62% coverage: 20:252/377 of query aligns to 4:236/369 of P19566
- L86 (= L102) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P176) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D181) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Sites not aligning to the query:
- 306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
43% identity, 62% coverage: 20:252/377 of query aligns to 3:235/374 of 2awnB
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
43% identity, 62% coverage: 20:252/377 of query aligns to 4:236/371 of P68187
- A85 (= A101) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ P122) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (= V130) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ M133) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ G135) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ Q140) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G153) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D174) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (= R244) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
Sites not aligning to the query:
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
43% identity, 62% coverage: 20:252/377 of query aligns to 3:235/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y29), S37 (= S54), G38 (= G55), C39 (= C56), G40 (= G57), K41 (= K58), S42 (= S59), T43 (= T60), Q81 (= Q98), R128 (= R145), A132 (≠ Q149), S134 (= S151), G136 (= G153), Q137 (= Q154), E158 (= E175), H191 (= H208)
- binding magnesium ion: S42 (= S59), Q81 (= Q98)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
43% identity, 62% coverage: 20:252/377 of query aligns to 3:235/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ Y29), G38 (= G55), C39 (= C56), G40 (= G57), K41 (= K58), S42 (= S59), T43 (= T60), R128 (= R145), S134 (= S151), Q137 (= Q154)
- binding beryllium trifluoride ion: S37 (= S54), G38 (= G55), K41 (= K58), Q81 (= Q98), S134 (= S151), G136 (= G153), H191 (= H208)
- binding magnesium ion: S42 (= S59), Q81 (= Q98)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
43% identity, 62% coverage: 20:252/377 of query aligns to 3:235/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ Y29), V17 (≠ A34), G38 (= G55), C39 (= C56), G40 (= G57), K41 (= K58), S42 (= S59), T43 (= T60), R128 (= R145), A132 (≠ Q149), S134 (= S151), Q137 (= Q154)
- binding tetrafluoroaluminate ion: S37 (= S54), G38 (= G55), K41 (= K58), Q81 (= Q98), S134 (= S151), G135 (= G152), G136 (= G153), E158 (= E175), H191 (= H208)
- binding magnesium ion: S42 (= S59), Q81 (= Q98)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
43% identity, 62% coverage: 20:252/377 of query aligns to 3:235/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ Y29), V17 (≠ A34), G38 (= G55), C39 (= C56), G40 (= G57), K41 (= K58), S42 (= S59), T43 (= T60), R128 (= R145), A132 (≠ Q149), S134 (= S151), Q137 (= Q154)
- binding magnesium ion: S42 (= S59), Q81 (= Q98)
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
43% identity, 62% coverage: 20:252/377 of query aligns to 1:233/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ Y29), S35 (= S54), G36 (= G55), C37 (= C56), G38 (= G57), K39 (= K58), S40 (= S59), T41 (= T60), R126 (= R145), A130 (≠ Q149), S132 (= S151), G134 (= G153), Q135 (= Q154)
1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
37% identity, 82% coverage: 22:329/377 of query aligns to 6:310/353 of 1oxvD
1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
37% identity, 82% coverage: 22:329/377 of query aligns to 6:310/353 of 1oxvA
1oxuA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
37% identity, 82% coverage: 22:329/377 of query aligns to 6:310/353 of 1oxuA
Q97UY8 Glucose import ATP-binding protein GlcV; EC 7.5.2.- from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
37% identity, 82% coverage: 22:329/377 of query aligns to 6:310/353 of Q97UY8
- S142 (= S151) mutation to A: Decrease in ATPase activity. Can form dimers.
- G144 (= G153) mutation to A: Loss of ATPase activity. Cannot form dimers. Forms an active heterodimer; when associated with A-166.
- E166 (= E175) mutation to A: Loss of ATPase activity. Can form dimers in the presence of ATP-Mg(2+). Forms an active heterodimer; when associated with A-144.; mutation to Q: Strong decrease in ATPase activity. Can form dimers in the presence of ATP alone, without Mg(2+).
Query Sequence
>14980 b0855 ATP-binding component of putrescine transport system (VIMSS)
MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYKGEIFALLGASGCGKST
LLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYALFPHMTVEQNIAFGLKQDK
LPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVALARSLAKRPKLLLLDEPMGAL
DKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAGRIAIMNRGKFVQIGEPEEIYEH
PTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLVHPLKVDADASVVDNVPVHVALRPE
KIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHVRLKSGQMISAQLQNAHRHRKGLPTWG
DEVRLCWEVDSCVVLTV
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory