SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 14996 FitnessBrowser__Keio:14996 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07003 Pyruvate dehydrogenase [ubiquinone]; Pyruvate oxidase; POX; Pyruvate:ubiquinone-8 oxidoreductase; EC 1.2.5.1 from Escherichia coli (strain K12) (see 4 papers)
100% identity, 100% coverage: 1:572/572 of query aligns to 1:572/572 of P07003

query
sites
P07003

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1:182 (vs. 1:182, 100% identical) Pyr domain
E50 (= E50) binding thiamine diphosphate
183:334 (vs. 183:334, 100% identical) FAD-binding domain
S210 (= S210) binding FAD
LR 234:235 (= LR 234:235) binding FAD
TGLI 251:254 (= TGLI 251:254) binding FAD
TQFPY 274:278 (= TQFPY 274:278) binding FAD
D292 (= D292) binding FAD
S297 (= S297) binding FAD
DI 311:312 (= DI 311:312) binding FAD
335:530 (vs. 335:530, 100% identical) PP-binding domain
T382 (= T382) binding thiamine diphosphate
FN 403:404 (= FN 403:404) binding FAD
GSM 406:408 (= GSM 406:408) binding thiamine diphosphate
D433 (= D433) binding Mg(2+)
DGG 433:435 (= DGG 433:435) binding thiamine diphosphate
N460 (= N460) binding Mg(2+)
460:466 (vs. 460:466, 100% identical) binding thiamine diphosphate
V462 (= V462) binding Mg(2+)
F465 (= F465) Moves into active site upon enzyme activation, plays a role in electron transfer
531:572 (vs. 531:572, 100% identical) Membrane-binding domain
A533 (= A533) mutation to T: In poxB11; poor activity in vivo, no longer activated by lipids.
YM 549:550 (= YM 549:550) In vitro cleavage to yield alpha-peptide
549:572 (vs. 549:572, 100% identical) mutation Missing: In poxB6. Inactive in vivo, does not complement inactive mutants. Active in vitro, no longer activated by nor binds to, detergents.
A553 (= A553) mutation to V: In poxB14; poor activity in vivo, no longer activated by lipids.
D560 (= D560) mutation to P: In poxB15; normal activity.
E564 (= E564) mutation to P: In poxB16; loss of activity, weakly activated by cleavage.
564:572 (vs. 564:572, 100% identical) mutation Missing: In poxB7 Inactive in vivo, reduced activity in vitro.
WLR 570:572 (= WLR 570:572) mutation Missing: In poxB8; reduced activity in vitro, not activated by lipids.
R572 (= R572) mutation to G: In poxB10; reduced activity in vivo and in vitro; may interact less with membranes.

3ey9A Structural basis for membrane binding and catalytic activation of the peripheral membrane enzyme pyruvate oxidase from escherichia coli (see paper)
100% identity, 100% coverage: 2:572/572 of query aligns to 1:571/571 of 3ey9A

query
sites
3ey9A

K

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E

E

P

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E

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R

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Q

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C

C

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M

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V

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Q

M

M

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K

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L

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P

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K

I

I

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V

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F

N

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L

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G

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V

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E

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A

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Y

Y

L

L

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D

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G

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E

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L

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H

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D

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T

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N

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F

A

A

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R

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I

A

A

E

E

A

A

C

C

G

G

I

I

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K

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active site: V23 (= V24), G25 (= G26), D26 (= D27), S27 (= S28), L28 (= L29), E49 (= E50), S72 (= S73), F111 (= F112), Q112 (= Q113), G160 (= G161), L252 (= L253), A279 (= A280), V379 (= V380), G405 (= G406), M407 (= M408), D432 (= D433), N459 (= N460), V461 (= V462), L462 (= L463), F464 (= F465), V465 (= V466), E468 (= E469), K528 (= K529)
binding flavin-adenine dinucleotide: G208 (= G209), S209 (= S210), G210 (= G211), A232 (= A233), L233 (= L234), R234 (= R235), T250 (= T251), G251 (= G252), I253 (= I254), G272 (= G273), T273 (= T274), Q274 (= Q275), F275 (= F276), Y277 (= Y278), D291 (= D292), I292 (= I293), S296 (= S297), G309 (= G310), D310 (= D311), I311 (= I312), T383 (= T384), F402 (= F403), N403 (= N404), Y548 (= Y549)
binding magnesium ion: D432 (= D433), N459 (= N460)
binding thiamine diphosphate: T24 (= T25), E49 (= E50), S72 (= S73), G76 (= G77), H79 (= H80), G380 (= G381), T381 (= T382), P382 (= P383), M407 (= M408), G431 (= G432), D432 (= D433), G433 (= G434), G434 (= G435), N459 (= N460), V461 (= V462), L462 (= L463), G463 (= G464)

1powA The refined structures of a stabilized mutant and of wild-type pyruvate oxidase from lactobacillus plantarum (see paper)
31% identity, 94% coverage: 7:543/572 of query aligns to 7:552/585 of 1powA

query
sites
1powA

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active site: I24 (≠ V24), G26 (= G26), G27 (≠ D27), S28 (= S28), I29 (≠ L29), E51 (= E50), S74 (= S73), F113 (= F112), Q114 (= Q113), E115 (= E114), V162 (≠ G161), R256 (≠ L253), E283 (vs. gap), V386 (= V380), A412 (≠ G406), M414 (= M408), D439 (= D433), N466 (= N460), Q468 (≠ V462), Y469 (≠ L463), F471 (= F465), I472 (≠ V466), E475 (= E469), G538 (≠ K529)
binding flavin-adenine dinucleotide: H93 (= H92), G212 (= G209), I213 (≠ S210), G214 (= G211), T236 (≠ A233), Y237 (≠ L234), A254 (≠ T251), V257 (≠ I254), G276 (= G273), N277 (≠ T274), N278 (≠ Q275), Y279 (≠ F276), P280 (= P277), F281 (≠ Y278), D298 (= D292), I299 (= I293), K303 (≠ S297), D317 (= D311), A318 (≠ I312), N409 (≠ F403)
binding magnesium ion: D439 (= D433), N466 (= N460), Q468 (≠ V462)
binding thiamine diphosphate: D388 (≠ T382), M414 (= M408), G440 (= G434), N466 (= N460), Q468 (≠ V462), Y469 (≠ L463), G470 (= G464), F471 (= F465), I472 (≠ V466)

4feeA High-resolution structure of pyruvate oxidase in complex with reaction intermediate 2-hydroxyethyl-thiamin diphosphate carbanion-enamine, crystal b (see paper)
31% identity, 94% coverage: 7:543/572 of query aligns to 7:552/586 of 4feeA

query
sites
4feeA

A

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F

V
x
I

A

K

M

D

E

E

M

Q

K

E

A

D

G

T

G

N

Y

Q

L

-

T

N

D

D

-

F

-

I

G

G

T

V

E

E

L

F

H

N

D

D

T

I

N

D

F

F

A

S

R

K

I

I

A

A

E

D

A

G

C

V

G

H

I

M

T

Q

G

A

I

F

R

R

V

V

E

N

K

K

A

I

S

E

E

Q

V

L

D

P

E

D

A

V

L

F

Q

E

R

Q

A

A

F

K

S

A

I

I

-

A

-

Q

D

H

G

E

P

P

V

V

L

L

V

I

D

D

V

A

V

V

V

I

A

T

K

G

E

D

E

R

L

P

A

L

I

P

P

A

P

E

Q

K

I
x
L

K

R

L
|
L

E
x
D

Q

S

A

A

binding flavin-adenine dinucleotide: H93 (= H92), G212 (= G209), I213 (≠ S210), G214 (= G211), T236 (≠ A233), Y237 (≠ L234), P238 (≠ R235), A254 (≠ T251), N255 (≠ G252), V257 (≠ I254), G276 (= G273), N277 (≠ T274), N278 (≠ Q275), P280 (= P277), F281 (≠ Y278), D298 (= D292), I299 (= I293), K303 (≠ S297), D317 (= D311), A318 (≠ I312), N390 (≠ T384), N409 (≠ F403)
binding magnesium ion: D439 (= D433), N466 (= N460), Q468 (≠ V462)
binding pyruvic acid: N255 (≠ G252), R256 (≠ L253), L547 (≠ I538), L549 (= L540), D550 (≠ E541)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V386 (= V380), D388 (≠ T382), A412 (≠ G406), M414 (= M408), G438 (= G432), G440 (= G434), N466 (= N460), Q468 (≠ V462), Y469 (≠ L463), G470 (= G464), F471 (= F465), I472 (≠ V466)

Sites not aligning to the query:

binding pyruvic acid: 553, 554

1v5gA Crystal structure of the reaction intermediate between pyruvate oxidase containing fad and tpp, and substrate pyruvate (see paper)
31% identity, 90% coverage: 2:514/572 of query aligns to 2:520/589 of 1v5gA

query
sites
1v5gA

K

K

Q

I

T

N

V

I

A

G

A

L

Y

A

I

V

A

M

K

K

T

I

L

L

E

E

S

S

A

W

G

G

V

A

K

D

R

T

I

I

W

Y

G

G

V

I

T

P

G

S

D

G

S

T

L

L

N

S

G

S

L

L

S

M

D

D

S

A

L

M

-

G

N

E

R

E

M

E

G

N

T

N

I

V

E

K

W

F

M

L

S

Q

T

V

R

K

H

H

E

E

V

V

A

G

A

A

F

M

A

A

G

V

A

M

E

Q

A

S

Q

K

L

F

S

G

G

G

E

N

L

L

A

G

V

V

C

T

A

V

G

G

S

S

C

G

G

G

P

P

G

G

N

A

L

S

H

H

L

L

I

I

N

N

G

G

L

L

F

Y

D

D

C

A

H

A

R

M

N

D

H

N

V

I

P

P

V

V

L

V

A

A

I

I

A

L

A

G

H

S

I

R

P

P

S

Q

S

R

E

E

I

L

G

N

S

M

G

D

Y

A

F

F

Q

Q

E

E

T

L

H

N

P

Q

Q

N

E

P

L

M

F

Y

R

D

E

H

C

I

S

A

H

V

Y

Y

C

N

E

R

L

R

V

V

S

A

S

Y

P

A

E

E

Q

Q

I

L

P

P

Q

K

V

L

L

V

A

D

I

E

A

A

M

A

R

R

K

M

A

A

V

I

L

A

N

K

R

R

G

G

V

V

S

A

V

V

V

L

V

E

L

V

P

P

G

G

D

D

V

F

A

A

L

-

K

-

P

-

A

K

P

V

E

E

G

I

A

D

T

N

M

D

H

Q

W

W

Y

Y

H

S

A

S

P

A

Q

N

P

S

V

L

-

R

-

K

-

Y

-

A

-

P

V

I

T

A

P

P

E

A

E

Q

E

D

L

I

R

D

K

A

L

A

A

V

Q

E

L

L

R

N

Y

N

S

S

S

K

N

R

I

P

A

V

L

I

M

Y

C

A

G
|
G

S
x
I

G
|
G

C

T

A

M

G

G

A

H

H

G

K

P

E

A

L

V

V

Q

E

E

F

L

A

A

G

R

K

K

I

I

K

K

A

A

P

P

I

V

V

I

H

T

A
x
T

L
x
G

R
x
K

G

N

K

F

E

E

H

T

V

F

E

E

Y

W

D

D

N

F

P

E

Y

A

D

L

V

T

G

G

M

S

T
|
T

G
x
Y

L
x
R

I
x
V

G

G

F

W

S

K

S

P

G

A

F

N

H

E

T

T

M

I

M

L

N

E

A

A

D

D

T

T

L

V

V

L

L

F

L

A

G
|
G

T
x
S

Q
x
N

F
|
F

P

P

Y
x
F

R

S

-

E

-

V

-

E

-

G

A

T

F

F

Y

R

P

N

T

V

D

D

A

-

K

N

I

F

I

I

Q

Q

I

I

D
|
D

I
|
I

N

D

P

P

A

A

S
x
M

I

L

G

G

A

K

H

R

S

H

K

H

V

A

D

D

M

V

A

A

L

I

V

L

G

G

D
|
D

I
x
A

K

A

S

L

T

A

L

I

R

D

A

E

L

I

L

L

P

N

L

K

V

V

E

D

E

A

K

V

A

E

D

E

R

S

K

A

F

W

L

W

D

T

K

A

A

N

L

L

E

K

D

N

Y

I

R

A

D

N

A

W

R

R

K

E

G

Y

L

I

D

N

D

M

L

L

A

E

K

T

P

K

S

E

E

E

K

G

A

D

I

L

H

Q

P

F

Q

Y

Y

Q

L

V

A

Y

Q

N

Q

A

I

I

S

N

H

N

F

H

A

A

A

D

D

E

D

D

A

A

I

I

F

Y

T

S

C

I

D

D

V
|
V

G

G

T
x
N

P

S

T

T

V

Q

W

T

A

S

A

I

R

R

Y

H

L

L

K

H

M

M

N

T

G

P

K

K

R

N

R

M

L

W

L

R

G

T

S

S

F
x
P

N

L

H

F

G

A

S

T

M
|
M

A

G

N

I

A

A

M

I

P

P

Q

G

A

G

L

L

G

G

A

A

Q

K

A

N

T

T

E

Y

P

P

E

D

R

R

Q

Q

V

V

V

W

A

N

M

I

C

I

G
|
G

D
|
D

G
|
G

G
x
A

F

F

S

S

M

M

L

T

M

Y

G

P

D

D

F

V

L

V

S

T

V

N

V

V

Q

R

M

Y

K

N

L

M

P

P

V

V

K

I

I

N

V

V

F

F

N

S

N
|
N

S

T

V
x
E

L
x
Y

G
x
A

F
|
F

V
x
I

A

K

M

N

E

K

M

Y

K

E

A

D

G

T

G

N

Y

K

L

N

T

L

D

F

G

G

T

V

E

D

L

F

H

T

D

D

T

V

N

D

F

Y

A

A

R

K

I

I

A

A

E

E

A

A

C

Q

G

G

I

A

T

K

G

G

I

F

R

T

V

V

E

S

K

R

A

I

S

E

E

D

V

M

D

D

E

R

A

V

L

M

Q

A

R

E

A

A

binding flavin-adenine dinucleotide: G212 (= G209), I213 (≠ S210), G214 (= G211), T236 (≠ A233), G237 (≠ L234), K238 (≠ R235), T254 (= T251), Y255 (≠ G252), R256 (≠ L253), V257 (≠ I254), G276 (= G273), S277 (≠ T274), N278 (≠ Q275), F279 (= F276), F281 (≠ Y278), D298 (= D292), I299 (= I293), M303 (≠ S297), D317 (= D311), A318 (≠ I312), P409 (≠ F403)
binding 2-acetyl-thiamine diphosphate: V386 (= V380), N388 (≠ T382), M414 (= M408), G438 (= G432), G440 (= G434), A441 (≠ G435), N466 (= N460), E468 (≠ V462), Y469 (≠ L463), A470 (≠ G464), F471 (= F465), I472 (≠ V466)
binding magnesium ion: D439 (= D433), N466 (= N460), E468 (≠ V462)

1v5fA Crystal structure of pyruvate oxidase complexed with fad and tpp, from aerococcus viridans (see paper)
31% identity, 90% coverage: 2:514/572 of query aligns to 2:520/589 of 1v5fA

query
sites
1v5fA

K

K

Q

I

T

N

V

I

A

G

A

L

Y

A

I

V

A

M

K

K

T

I

L

L

E

E

S

S

A

W

G

G

V

A

K

D

R

T

I

I

W

Y

G

G

V

I

T

P

G

S

D

G

S

T

L

L

N

S

G

S

L

L

S

M

D

D

S

A

L

M

-

G

N

E

R

E

M

E

G

N

T

N

I

V

E

K

W

F

M

L

S

Q

T

V

R

K

H

H

E

E

V

V

A

G

A

A

F

M

A

A

G

V

A

M

E

Q

A

S

Q

K

L

F

S

G

G

G

E

N

L

L

A

G

V

V

C

T

A

V

G

G

S

S

C

G

G

G

P

P

G

G

N

A

L

S

H

H

L

L

I

I

N

N

G

G

L

L

F

Y

D

D

C

A

H

A

R

M

N

D

H

N

V

I

P

P

V

V

L

V

A

A

I

I

A

L

A

G

H

S

I

R

P

P

S

Q

S

R

E

E

I

L

G

N

S

M

G

D

Y

A

F

F

Q

Q

E

E

T

L

H

N

P

Q

Q

N

E

P

L

M

F

Y

R

D

E

H

C

I

S

A

H

V

Y

Y

C

N

E

R

L

R

V

V

S

A

S

Y

P

A

E

E

Q

Q

I

L

P

P

Q

K

V

L

L

V

A

D

I

E

A

A

M

A

R

R

K

M

A

A

V

I

L

A

N

K

R

R

G

G

V

V

S

A

V

V

V

L

V

E

L

V

P

P

G

G

D

D

V

F

A

A

L

-

K

-

P

-

A

K

P

V

E

E

G

I

A

D

T

N

M

D

H

Q

W

W

Y

Y

H

S

A

S

P

A

Q

N

P

S

V

L

-

R

-

K

-

Y

-

A

-

P

V

I

T

A

P

P

E

A

E

Q

E

D

L

I

R

D

K

A

L

A

A

V

Q

E

L

L

R

N

Y

N

S

S

S

K

N

R

I

P

A

V

L

I

M

Y

C

A

G
|
G

S
x
I

G
|
G

C

T

A

M

G

G

A

H

H

G

K

P

E

A

L

V

V

Q

E

E

F

L

A

A

G

R

K

K

I

I

K

K

A

A

P

P

I

V

V

I

H

T

A
x
T

L
x
G

R
x
K

G

N

K

F

E

E

H

T

V

F

E

E

Y

W

D

D

N

F

P

E

Y

A

D

L

V

T

G

G

M

S

T
|
T

G
x
Y

L
x
R

I
x
V

G

G

F

W

S

K

S

P

G

A

F

N

H

E

T

T

M

I

M

L

N

E

A

A

D

D

T

T

L

V

V

L

L

F

L

A

G
|
G

T
x
S

Q
x
N

F
|
F

P
|
P

Y
x
F

R

S

-

E

-

V

-

E

-

G

A

T

F

F

Y

R

P

N

T

V

D

D

A

-

K

N

I

F

I

I

Q

Q

I

I

D
|
D

I
|
I

N

D

P

P

A

A

S
x
M

I

L

G

G

A

K

H

R

S

H

K

H

V

A

D

D

M

V

A

A

L

I

V

L

G

G

D
|
D

I
x
A

K

A

S

L

T

A

L

I

R

D

A

E

L

I

L

L

P

N

L

K

V

V

E

D

E

A

K

V

A

E

D

E

R

S

K

A

F

W

L

W

D

T

K

A

A

N

L

L

E

K

D

N

Y

I

R

A

D

N

A

W

R

R

K

E

G

Y

L

I

D

N

D

M

L

L

A

E

K

T

P

K

S

E

E

E

K

G

A

D

I

L

H

Q

P

F

Q

Y

Y

Q

L

V

A

Y

Q

N

Q

A

I

I

S

N

H

N

F

H

A

A

A

D

D

E

D

D

A

A

I

I

F

Y

T

S

C

I

D

D

V

V

G

G

T
x
N

P
x
S

T

T

V

Q

W

T

A

S

A

I

R

R

Y

H

L

L

K

H

M

M

N

T

G

P

K

K

R

N

R

M

L

W

L

R

G

T

S

S

F
x
P

N

L

H

F

G

A

S

T

M
|
M

A

G

N

I

A

A

M

I

P

P

Q

G

A

G

L

L

G

G

A

A

Q

K

A

N

T

T

E

Y

P

P

E

D

R

R

Q

Q

V

V

V

W

A

N

M

I

C

I

G
|
G

D
|
D

G
|
G

G

A

F

F

S

S

M

M

L

T

M

Y

G

P

D

D

F

V

L

V

S

T

V

N

V

V

Q

R

M

Y

K

N

L

M

P

P

V

V

K

I

I

N

V

V

F

F

N

S

N
|
N

S

T

V

E

L
x
Y

G
x
A

F
|
F

V
x
I

A

K

M

N

E

K

M

Y

K

E

A

D

G

T

G

N

Y

K

L

N

T

L

D

F

G

G

T

V

E

D

L

F

H

T

D

D

T

V

N

D

F

Y

A

A

R

K

I

I

A

A

E

E

A

A

C

Q

G

G

I

A

T

K

G

G

I

F

R

T

V

V

E

S

K

R

A

I

S

E

E

D

V

M

D

D

E

R

A

V

L

M

Q

A

R

E

A

A

binding flavin-adenine dinucleotide: G212 (= G209), I213 (≠ S210), G214 (= G211), T236 (≠ A233), G237 (≠ L234), K238 (≠ R235), T254 (= T251), Y255 (≠ G252), R256 (≠ L253), V257 (≠ I254), G276 (= G273), S277 (≠ T274), N278 (≠ Q275), F279 (= F276), P280 (= P277), F281 (≠ Y278), D298 (= D292), I299 (= I293), M303 (≠ S297), D317 (= D311), A318 (≠ I312), P409 (≠ F403)
binding magnesium ion: D439 (= D433), N466 (= N460)
binding thiamine diphosphate: N388 (≠ T382), S389 (≠ P383), M414 (= M408), G438 (= G432), G440 (= G434), N466 (= N460), Y469 (≠ L463), A470 (≠ G464), F471 (= F465), I472 (≠ V466)

2djiA Crystal structure of pyruvate oxidase from aerococcus viridans containing fad (see paper)
31% identity, 90% coverage: 2:514/572 of query aligns to 3:521/590 of 2djiA

query
sites
2djiA

K

K

Q

I

T

N

V

I

A

G

A

L

Y

A

I

V

A

M

K

K

T

I

L

L

E

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