SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 15246 FitnessBrowser__Keio:15246 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

8hpsB ABC transporter, permease protein SugB (see paper)
26% identity, 58% coverage: 47:199/264 of query aligns to 52:204/264 of 8hpsB

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binding alpha-D-glucopyranose: H113 (≠ D108), F200 (≠ L195)

P94530 Arabinooligosaccharides transport system permease protein AraQ from Bacillus subtilis (strain 168) (see paper)
28% identity, 67% coverage: 10:187/264 of query aligns to 19:202/281 of P94530

query
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P94530

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D180 (≠ L165) mutation to A: Negative impact in the growth rate in the presence of arabinotriose.

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
28% identity, 54% coverage: 62:204/264 of query aligns to 87:234/296 of P68183

query
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P68183

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E190 (= E160) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A162) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G166) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P179) mutation to A: No effect.

Query Sequence

>15246 FitnessBrowser__Keio:15246
MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTTKWYSLLMNNDSLLQAA
QHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFVVMMSPDIVMAISLLVLFM
LLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLEAAKDLGASEFTILRKIILPL
AMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKIYSMVKVGVSPEVNALATILLVL
SLVMVIASQLIARDKTKGNTGDVK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory