SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 15248 b1126 putrescine/spermidine ABC transporter ATPase protein (NCBI) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
100% identity, 100% coverage: 1:378/378 of query aligns to 1:378/378 of P69874

query
sites
P69874

M

M

G

G

Q

Q

S

S

K

K

L

L

N

N

K

K

Q

Q

P

P

S

S

L

L

S

S

P

P

L

L

V

V

Q

Q

L

L

A

A

G

G

I

I

R

R

K

K

C
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C

F
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F

D

D

G

G

K

K

E

E

V

V

I

I

P

P

Q

Q

L

L

D

D

L

L

T

T

I

I

N

N

G

G

E

E

F
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F

L

L

T

T

L

L

G

G

P

P

S

S

G

G

C
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C

G

G

K

K

T

T

V

V

L
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L

R

R

L

L

I

I

A

A

G

G

L

L

E

E

T

T

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V

D

D

S

S

G

G

R

R

I

I

M

M

L
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L

D

D

N

N

E

E

D

D

I

I

T

T

H

H

V

V

P

P

A

A

E

E

N

N

R

R

Y

Y

V

V

N

N

T

T

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

F

P

P

H

H

M

M

T

T

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V

F

F

E

E

N

N

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V

A

A

F

F

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G

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L

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R

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M

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Q

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K

T

T

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A

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E

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M

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A

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V

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Q

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L

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E

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T

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F

A

A

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R

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K

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P

H

H

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L

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G

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Q

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I

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A

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A

A

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V

N

N

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K

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D

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E

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S

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L

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S

A

A

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L

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D

Y

Y

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K

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L

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R

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K

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M

M

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Q

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N

E

E

L

L

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K

A

A

L

L

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Q

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R

K

K

L

L

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G

I

I

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T

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F

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V

F

F

V

V

T

T

H

H

D

D

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Q

E

E

A

A

L

L

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T

M

M

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S

D

D

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R

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I

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V

M

M

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R

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D

G

G

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R

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I

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E

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Q

D

D

G

G

T

T

P

P

R

R

E

E

I

I

Y

Y

E

E

P

P

K

K

N

N

L

L

F

F

V

V

A

A

G

G

F

F

I

I

G

G

E

E

I

I

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N

M

M

F

F

N

N

A

A

T

T

V

V

I

I

E

E

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R

L

L

D

D

E

E

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Q

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R

V

V

R

R

A

A

N

N

V

V

E

E

G

G

R

R

E

E

C
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C

N

N

I

I

Y

Y

V

V

N

N

F

F

A

A

V

V

E

E

P

P

G

G

Q

Q

K

K

L

L

H

H

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V

L

L

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R

P

P

E
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E

D

D

L

L

R

R

V

V

E

E

I

I

N

N

D

D

N

N

H

H

A

A

E

E

G

G

L

L

I

I

G

G

Y

Y

V

V

R

R

E

E

R

R

N

N

Y

Y

K

K

G

G

M

M

T

T

L

L

E

E

S

S

V

V

E

E

L

L

E

E

N

N

G

G

K

K

M

M

V

V

M

M

V

V

S

S

E

E

F

F

N

N

E

E

D

D

P

P

D

D

F

F

D

D

H

H

S

S

L

L

D

D

Q

Q

K

K

M

M

A

A

I

I

N

N

W

W

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V

E

E

S

S

W

W

E

E

V

V

L

L

A

A

D

D

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E

H

H

K

K

C26 (= C26) mutation to A: Lower ATPase activity and transport efficiency.
F27 (= F27) mutation to L: Lower ATPase activity and transport efficiency.
F45 (= F45) mutation to L: Lower ATPase activity and transport efficiency.
C54 (= C54) mutation to T: Loss of ATPase activity and transport.
L60 (= L60) mutation to F: Lower ATPase activity and transport efficiency.
L76 (= L76) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V135) mutation to M: Loss of ATPase activity and transport.
D172 (= D172) mutation to N: Loss of ATPase activity and transport.
C276 (= C276) mutation to A: Lower ATPase activity and transport efficiency.
E297 (= E297) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
52% identity, 64% coverage: 18:260/378 of query aligns to 7:257/375 of 2d62A

query
sites
2d62A

V

V

Q

K

L

L

A

I

G

N

I

I

R

W

K

K

C

R

F

F

D

G

G

D

K

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E

T

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A

I

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K

Q

D

L

L

D

S

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L

T

E

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I

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K

N

D

G

G

E

E

F

F

L

L

T

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L

L

G

G

P
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P

S
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S

G
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G

C
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C

G
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G

K
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K

T
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T

V

T

L

L

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R

L

M

I

I

A

A

G

G

L

L

E

E

T

E

V

P

D

T

S

R

G

G

R

Q

I

I

M

Y

L

I

-

E

-

D

-

N

-

L

-

V

-

A

D

D

N

P

E

E

D

K

I

G

T

V

H

F

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V

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P

A

P

E

K

N

E

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R

Y

D

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V

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A

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M

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V

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F

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S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

V

V

F

Y

E

D

N

N

V

I

A

A

F

F

G

P

L

L

R

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M

L

Q

R

K

K

T

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P

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A

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A

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E

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K

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R

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M

R

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E

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A

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E

M

M

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L

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G

L

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E

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T

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F

L

A

L

Q

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R

R

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K

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P

H

R

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E

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L

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S

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G

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Q

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Q

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A

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A

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R

A

A

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N

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R

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L

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L

F

L

L

L

M

D

D

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E

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L

L

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S

A

N

L

L

D

D

Y

A

K

K

L

L

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Q

K

M

M

Q

R

N

A

E

E

L

L

K

K

A

K

L

L

Q

Q

R

R

K

Q

L

L

G

G

I

V

T

T

F

T

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

L

M

T

T

M

M

S

G

D

D

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R

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I

V

A

V

V

M

M

R

N

D

K

G

G

R

E

I

L

E

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Q

Q

D

V

G

G

T

T

P

P

R

D

E

E

I

V

Y

Y

E

Y

E

K

P

P

K

V

N

N

L

T

F

F

V

V

A

A

G

G

F

F

I

I

G

G

E

S

-

P

I

M

N

N

M

F

F

L

N

D

A

A

T

T

V

I

I

T

E

D

binding pyrophosphate 2-: P40 (= P51), S41 (= S52), G42 (= G53), C43 (= C54), G44 (= G55), K45 (= K56), T46 (= T57), T47 (= T58)

1g291 Malk (see paper)
51% identity, 64% coverage: 18:260/378 of query aligns to 4:254/372 of 1g291

query
sites
1g291

V

V

Q

R

L

L

A

V

G

D

I

V

R

W

K

K

C

V

F

F

D

G

G

E

K

V

E

T

V

A

I

V

P

R

Q

E

L

M

D

S

L

L

T

E

I

V

N

K

N

D

G

G

E

E

F

F

L

M

T

I

L

L

G

G

P
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P

S
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S

G
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G

C
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C

G
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G

K
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K

T
|
T

V

T

L

L

R

R

L

M

I

I

A

A

G

G

L

L

E

E

T

E

V

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D

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S

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G

R

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I

M

Y

L

I

-

G

-

D

-

K

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L

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D

N

P

E
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E

D
x
K

I

G

T

I

H

F

V

V

P

P

A

P

E
x
K

N
x
D

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Y

D

V

I

N

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M

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V

F

F

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Q

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S

Y

Y

A

A

L

L

F

Y

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P

H

H

M

M

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T

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V

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Y

E

D

N

N

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I

A

A

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L

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R

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L

F

L

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M

D

D

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L

L

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S

A

N

L

L

D

D

Y

A

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K

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L

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Q

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M

M

Q

R

N

A

E

E

L

L

K

K

A

K

L

L

Q

Q

R

R

K

Q

L

L

G

G

I

V

T

T

F

T

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

L

M

T

T

M

M

S

G

D

D

R

R

I

I

V

A

V

V

M

M

R

N

D

R

G

G

R

V

I

L

E

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Q

Q

D

V

G

G

T

S

P

P

R

D

E

E

I

V

Y
|
Y

E
x
D

E

K

P

P

K

A

N

N

L

T

F

F

V

V

A

A

G

G

F

F

I

I

G

G

E

S

-

P

I

M

N

N

M

F

F

L

N

D

A

A

T

I

V

V

I

T

E

E

binding magnesium ion: D69 (= D77), E71 (= E79), K72 (≠ D80), K79 (≠ E87), D80 (≠ N88), Y228 (= Y236), D229 (≠ E237)
binding pyrophosphate 2-: P37 (= P51), S38 (= S52), G39 (= G53), C40 (= C54), G41 (= G55), K42 (= K56), T43 (= T57), T44 (= T58)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 341, 342, 359

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
53% identity, 61% coverage: 28:256/378 of query aligns to 15:245/393 of P9WQI3

query
sites
P9WQI3

D

D

G

G

K

H

E

T

V

A

I

V

P

R

Q

D

L

L

D

N

L

L

T

T

I

I

N

A

N

D

G

G

E

E

F

F

L

L

T

I

L

L

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

T

V

T

L

L

R

N

L

M

I

I

A

A

G

G

L

L

E

E

T

D

V

I

D

S

S

S

G

G

R

E

I

L

M

R

L

I

D

A

N

G

E

E

D

R

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N

H

E

V

K

P

A

A

P

E

K

N

D

R

R

Y

D

V

I

N

A

T

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

V

V

F

R

E

Q

N

N

V

I

A

A

F

F

G

P

L

L

R

T

M

L

Q

A

K

K

T

M

P

R

A

K

A

A

E

D

I

I

T

A

P

Q

R

K

V

V

M

S

E

E

A

T

L

A

R

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M

I

V

L

Q

D

L

L

E

T

T

N

F

L

A

L

Q

D

R

R

K

K

P

P

H

S

Q

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

M

A

G

R

R

A

A

V

I

V

V

N

R

K

H

P

P

R

K

L

A

L

F

L

L

L

M

D

D

E

E

S

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

K

L

L

R

R

K

V

Q

Q

M

M

Q

R

N

G

E

E

L

I

K

A

A

Q

L

L

Q

Q

R

R

K

R

L

L

G

G

I

T

T

T

F

T

V

V

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

T

E

E

A

A

L

M

T

T

M

L

S

G

D

D

R

R

I

V

V

V

M

M

R

Y

D

G

G

G

R

I

I

A

E

Q

Q

Q

D

I

G

G

T

T

P

P

R

E

E

E

I

L

Y

Y

E

E

E

R

P

P

K

A

N

N

L

L

F

F

V

V

A

A

G

G

F

F

I

I

G

G

E

S

-

P

-

A

I

M

N

N

M

F

F

F

N

P

A

A

H193 (= H206) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

7cagC Mycobacterium smegmatis lpqy-sugabc complex in the catalytic intermediate state (see paper)
52% identity, 60% coverage: 33:257/378 of query aligns to 16:242/362 of 7cagC

query
sites
7cagC

I

V

P

K

Q

E

L

F

D

S

L

M

T

T

I

I

N

A

N

D

G

G

E

E

F

F

L

I

T

I

L

L

L

V

G

G

P
|
P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

V

T

L

L

R

N

L

M

I

I

A

A

G

G

L

L

E

E

T

E

V

I

D

T

S

S

G

G

R

E

I

L

M

R

L

I

D

G

N

G

E

E

D

R

I

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T

N

H

E

V

K

P

A

A

P

E

K

N

D

R

R

Y

D

V

I

N

A

T

M

V

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

V

V

F

R

E

Q

N

N

V

I

A

A

F

F

G

P

L

L

R

T

M

L

Q

A

K

K

T

V

P

P

A

K

A

A

E

E

I

I

T

A

P

A

R

K

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V

M

E

E

E

A

T

L

A

R

K

M

I

V

L

Q

D

L

L

E

S

T

E

F
x
L

A

L

Q

D

R
|
R

K

K

P

P

H

G

Q
|
Q

L

L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

M

A

G

R

R

A

A

V

I

V

V

N

R

K

S

P

P

R

K

L

A

L

F

L

L

L

M

D

D

E

Q

S

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

K

L

L

R

R

K

V

Q

Q

M

M

Q

R

N

A

E

E

L

I

K

S

A

R

L

L

Q

Q

R

D

K

R

L

L

G

G

I

T

T

T

F

T

V

V

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

T

E

E

A

A

L

M

T

T

M

L

S

G

D

D

R

R

I

V

V

V

M

M

R

L

D

A

G

G

R

E

I

V

E

Q

Q

Q

D

I

G

G

T

T

P

P

R

D

E

E

I

L

Y

Y

E

S

P

P

K

A

N

N

L

L

F

F

V

V

A

A

G

G

F

F

I

I

G

G

E

S

-

P

-

A

I

M

N

N

M

F

F

F

N

P

A

A

T

T

binding adenosine-5'-triphosphate: P34 (= P51), S35 (= S52), G36 (= G53), C37 (= C54), G38 (= G55), K39 (= K56), S40 (≠ T57), T41 (= T58), Q79 (= Q96), L123 (≠ F140), R126 (= R143), Q130 (= Q147), S132 (= S149), G133 (= G150), G134 (= G151), Q135 (= Q152)
binding magnesium ion: S40 (≠ T57), Q79 (= Q96)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 14

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
46% identity, 79% coverage: 18:317/378 of query aligns to 7:295/353 of 1vciA

query
sites
1vciA

V

V

Q

K

L

L

A

E

G

N

I

L

R

T

K

K

C

R

F
|
F

D

G

G

N

K
x
F

E

T

V

A

I

V

P

N

Q

K

L

L

D

N

L

L

T

T

I

I

N

K

N

D

G

G

E

E

F

F

L

L

T

V

L

L

G

G

P
|
P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
|
T

V

T

L

L

R

R

L

M

I

I

A

A

G

G

L

L

E

E

T

E

V

P

D

T

S

E

G

G

R

R

I

I

M

Y

L

F

D

G

N

D

E

R

D

D

I

V

T

T

H

Y

V

L

P

P

A

P

E

K

N

D

R

R

Y

N

V

I

N

S

T

M

V

V

F

F

Q

Q

S

-

Y

-

A

-

L

-

F

-

P

-

H

H

M

M

T

T

V

V

F

Y

E

E

N

N

V

I

A

A

F

F

G

P

L

L

R

K

M

-

Q

-

K

K

T

F

P

P

A

K

A

D

E

E

I

I

T

D

P

K

R

R

V

V

M

R

E

W

A

A

L

A

R

E

M

L

V

L

Q

Q

L

I

E

E

T

E

F

L

A

L

Q

N

R

R

K

Y

P

P

H

A

Q

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

V

A

A

R

R

A

A

V

I

V

V

N

V

K

E

P

P

R

D

L

V

L

L

L

M

D

D

E

E

S

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

K

L

L

R

R

K

V

Q

A

M

M

Q

R

N

A

E

E

L

I

K

K

A

K

L

L

Q

Q

R

Q

K

K

L

L

G

K

I

V

T

T

F

T

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

L

M

T

T

M

M

S

G

D

D

R

R

I

I

V

A

V

V

M

M

R

N

D

R

G

G

R

Q

I

L

E

L

Q

Q

D

I

G

G

T

S

P

P

R

T

E

E

I

V

Y

Y

E

L

E

R

P

P

K

N

N

S

L

V

F

F

V

V

A

A

G

T

F

F

I

I

G

G

-

A

-

P

E

E

I

M

N

N

M

I

F

L

N

E

A

V

T

S

V

V

I

G

E

D

R

G

L

Y

D

L

E

E

Q

G

R

R

V

-

R

G

A

F

N

R

V

I

E

E

G

L

R

P

E

Q

C

M

N

D

I

L

Y

L

V

K

N

D

F

Y

A

V

V

-

E

-

P

-

G

G

Q

K

K

T

L

V

H

L

V

F

L

G

L

I

R

R

P

P

E

E

D

H

L

M

R

T

V

V

E

E

E

G

I

V

N

H

D

M

D

K

N

R

H

T

A

A

E

R

G

-

L

L

I

I

G

G

Y

K

V

V

binding adenosine-5'-triphosphate: F16 (= F27), F19 (≠ K30), P40 (= P51), S41 (= S52), G42 (= G53), C43 (= C54), G44 (= G55), K45 (= K56), T46 (= T57), T47 (= T58)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
43% identity, 76% coverage: 18:306/378 of query aligns to 4:295/369 of P19566

query
sites
P19566

V

V

Q

Q

L

L

A

R

G

N

I

V

R

T

K

K

C

A

F

W

D

G

G

D

K

V

E

V

V

V

I

S

P

K

Q

D

L

I

D

N

L

L

T

D

I

I

N

H

N

D

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

V

L

L

L

R

R

L

M

I

I

A

A

G

G

L

L

E

E

T

T

V

I

D

T

S

S

G

G

R

D

I

L

M

F

L

I

D

G

N

E

E

T

D

R

I

M

T

N

H

D

V

I

P

P

A

P

E

A

N

E

R

R

Y

G

V

V

N

G

T

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L
|
L

F

Y

P

P

H

H

M

L

T

S

V

V

F

A

E

E

N

N

V

M

A

S

F

F

G

G

L

L

R

K

M

L

Q

A

K

G

T

A

P

K

A

K

A

E

E

V

I

M

T

N

P

Q

R

R

V

V

M

N

E

Q

A

V

L

A

R

E

M

V

V

L

Q

Q

L

L

E

A

T

H

F

L

A

L

Q

E

R

R

K

K

P

P

H

K

Q

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

N

A

K

E

P

P

R

R

L

V

L

F

L

L

D

D

E

E

S
x
P

L

L

S

S

A

N

L

L

D
|
D

Y

A

K

A

L

L

R

R

K

V

Q

Q

M

M

Q

R

N

I

E

E

L

I

K

S

A

R

L

L

Q

H

R

K

K

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

I

I

V

V

M

L

R

D

D

A

G

G

R

R

I

V

E

A

Q

Q

D

V

G

G

T

K

P

P

R

L

E

E

I

L

Y

Y

E

H

E

Y

P

P

K

A

N

D

L

R

F

F

V

V

A

A

G

G

F

F

I

I

G

G

E

S

I

P

N

K

M

M

-

N

-

F

-

L

-

P

-

V

-

K

F

V

N

T

A

A

T

T

V

A

I

I

E

E

R

Q

L

V

-

Q

-

V

-

E

-

L

-

P

D

N

E

R

Q

Q

R

Q

V

I

R

W

A

L

N

P

V

V

E

E

G

S

R

R

E

-

C

-

N

-

I

-

Y

-

V

-

N

-

F

-

A

G

V

V

E

Q

P

V

G

G

Q

A

K

N

L

M

H

S

V

L

L

G

L

I

R

R

P

P

E

E

D

H

L

L

R

L

V

P

E

S

E

D

I

I

N

A

D

D

L86 (= L100) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (≠ S174) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D179) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

2awoA Crystal structure of the adp-mg-bound e. Coli malk (crystallized with adp-mg) (see paper)
42% identity, 76% coverage: 18:306/378 of query aligns to 3:296/372 of 2awoA

query
sites
2awoA

V

V

Q

Q

L

L

A

Q

G

N

I

V

R

T

K

K

C

A

F
x
W

D

G

G

E

K

V

E

V

V
|
V

I

S

P

K

Q

D

L

I

D

N

L

L

T

D

I

I

N

H

N

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

V

L

L

L

R

R

L

M

I

I

A

A

G

G

L

L

E

E

T

T

V

I

D

T

S

S

G

G

R

D

I

L

M

F

L

I

D

G

N

E

E

K

D

R

I

M

T

N

H

D

V

T

P

P

A

P

E

A

N

E

R

R

Y

G

V

V

N

G

T

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

F

A

E

E

N

N

V

M

A

S

F

F

G

G

L

L

R

K

M

L

Q

A

K

G

T

A

P

K

A

K

A

E

E

V

I

I

T

N

P

Q

R

R

V

V

M

N

E

Q

A

V

L

A

R

E

M

V

V

L

Q

Q

L

L

E

A

T

H

F

L

A

L

Q

D

R

R

K

K

P

P

H

K

Q

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

N

A

K

E

P

P

R

S

L

V

L

F

L

L

D
|
D

E

E

S

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

A

L

L

R

R

K

V

Q

Q

M

M

Q

R

N

I

E

E

L

I

K

S

A

R

L

L

Q

H

R

K

K

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

I

I

V

V

M

L

R

D

D

A

G

G

R

R

I

V

E

A

Q

Q

D

V

G

G

T

K

P

P

R

L

E

E

I

L

Y

Y

E

H

E

Y

P

P

K

A

N

D

L

R

F

F

V

V

A

A

G

G

F

F

I

I

G

G

E

S

I

P

N

K

M

M

-

N

-

F

-

L

-

P

-

V

-

K

F

V

N

T

A

A

T

T

V

A

I

I

E

D

R

Q

L

V

-

Q

-

V

-

E

-

L

-

P

-

M

-

P

D

N

E

R

Q

Q

R

Q

V

V

R

W

A

L

N

P

V

V

E

E

G

S

R

R

E

D

C

V

N

Q

I

V

Y

G

V

A

N

N

F

M

A

S

V

L

E

G

P

-

G

-

Q

-

K

-

L

-

H

-

V

-

L

-

L

I

R

R

P

P

E

E

D

H

L

L

R

L

V

P

E

S

E

D

I

I

N

A

D

D

binding adenosine-5'-diphosphate: W12 (≠ F27), V17 (= V32), S37 (= S52), G38 (= G53), C39 (= C54), G40 (= G55), K41 (= K56), S42 (≠ T57), T43 (= T58)
binding magnesium ion: S42 (≠ T57), D157 (= D172)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
42% identity, 76% coverage: 18:306/378 of query aligns to 1:294/367 of 1q12A

query
sites
1q12A

V

V

Q

Q

L

L

A

Q

G

N

I

V

R

T

K

K

C

A

F
x
W

D

G

G

E

K

V

E

V

V
|
V

I

S

P

K

Q

D

L

I

D

N

L

L

T

D

I

I

N

H

N

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

V

L

L

L

R

R

L

M

I

I

A

A

G

G

L

L

E

E

T

T

V

I

D

T

S

S

G

G

R

D

I

L

M

F

L

I

D

G

N

E

E

K

D

R

I

M

T

N

H

D

V

T

P

P

A

P

E

A

N

E

R

R

Y

G

V

V

N

G

T

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

F

A

E

E

N

N

V

M

A

S

F

F

G

G

L

L

R

K

M

L

Q

A

K

G

T

A

P

K

A

K

A

E

E

V

I

I

T

N

P

Q

R

R

V

V

M

N

E

Q

A

V

L

A

R

E

M

V

V

L

Q

Q

L

L

E

A

T

H

F

L

A

L

Q

D

R
|
R

K

K

P

P

H
x
K

Q
x
A

L
|
L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

N

A

K

E

P

P

R

S

L

V

L

F

L

L

D

D

E

E

S

P

L

L

S

S

A
x
N

L

L

D

D

Y

A

K

A

L

L

R

R

K

V

Q

Q

M

M

Q

R

N

I

E

E

L

I

K

S

A

R

L

L

Q

H

R

K

K

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

I

I

V

V

M

L

R

D

D

A

G

G

R

R

I

V

E

A

Q

Q

D

V

G

G

T

K

P

P

R

L

E

E

I

L

Y

Y

E

H

E

Y

P

P

K

A

N

D

L

R

F

F

V

V

A

A

G

G

F

F

I

I

G

G

E

S

I

P

N

K

M

M

-

N

-

F

-

L

-

P

-

V

-

K

F

V

N

T

A

A

T

T

V

A

I

I

E

D

R

Q

L

V

-

Q

-

V

-

E

-

L

-

P

-

M

-

P

D

N

E

R

Q

Q

R

Q

V

V

R

W

A

L

N

P

V

V

E

E

G

S

R

R

E

D

C

V

N

Q

I

V

Y

G

V

A

N

N

F

M

A

S

V

L

E

G

P

-

G

-

Q

-

K

-

L

-

H

-

V

-

L

-

L

I

R

R

P

P

E

E

D

H

L

L

R

L

V

P

E

S

E

D

I

I

N

A

D

D

binding adenosine-5'-triphosphate: W10 (≠ F27), V15 (= V32), S35 (= S52), G36 (= G53), C37 (= C54), G38 (= G55), K39 (= K56), S40 (≠ T57), T41 (= T58), R126 (= R143), K129 (≠ H146), A130 (≠ Q147), L131 (= L148), S132 (= S149), G133 (= G150), G134 (= G151), Q135 (= Q152), N160 (≠ A177), H189 (= H206)

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
42% identity, 76% coverage: 18:306/378 of query aligns to 3:296/371 of 3puyA

query
sites
3puyA

V

V

Q

Q

L

L

A

Q

G

N

I

V

R

T

K

K

C

A

F
x
W

D

G

G

E

K

V

E

V

V
|
V

I

S

P

K

Q

D

L

I

D

N

L

L

T

D

I

I

N

H

N

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P
|
P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

V

L

L

L

R

R

L

M

I

I

A

A

G

G

L

L

E

E

T

T

V

I

D

T

S

S

G

G

R

D

I

L

M

F

L

I

D

G

N

E

E

K

D

R

I

M

T

N

H

D

V

T

P

P

A

P

E

A

N

E

R

R

Y

G

V

V

N

G

T

M

V

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

F

A

E

E

N

N

V

M

A

S

F

F

G

G

L

L

R

K

M

L

Q

A

K

G

T

A

P

K

A

K

A

E

E

V

I

I

T

N

P

Q

R

R

V

V

M

N

E

Q

A

V

L

A

R

E

M

V

V

L

Q

Q

L

L

E

A

T

H

F

L

A

L

Q

D

R
|
R

K

K

P

P

H

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

N

A

K

E

P

P

R

S

L

V

L

F

L

L

D
|
D

E
|
E

S

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

A

L

L

R

R

K

V

Q

Q

M

M

Q

R

N

I

E

E

L

I

K

S

A

R

L

L

Q

H

R

K

K

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

I

I

V

V

M

L

R

D

D

A

G

G

R

R

I

V

E

A

Q

Q

D

V

G

G

T

K

P

P

R

L

E

E

I

L

Y

Y

E

H

E

Y

P

P

K

A

N

D

L

R

F

F

V

V

A

A

G

G

F

F

I

I

G

G

E

S

I

P

N

K

M

M

-

N

-

F

-

L

-

P

-

V

-

K

F

V

N

T

A

A

T

T

V

A

I

I

E

D

R

Q

L

V

-

Q

-

V

-

E

-

L

-

P

-

M

-

P

D

N

E

R

Q

Q

R

Q

V

V

R

W

A

L

N

P

V

V

E

E

G

S

R

R

E

D

C

V

N

Q

I

V

Y

G

V

A

N

N

F

M

A

S

V

L

E

G

P

-

G

-

Q

-

K

-

L

-

H

-

V

-

L

-

L

I

R

R

P

P

E

E

D

H

L

L

R

L

V

P

E

S

E

D

I

I

N

A

D

D

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F27), V17 (= V32), P36 (= P51), S37 (= S52), G38 (= G53), C39 (= C54), G40 (= G55), K41 (= K56), S42 (≠ T57), T43 (= T58), Q81 (= Q96), R128 (= R143), A132 (≠ Q147), S134 (= S149), G135 (= G150), G136 (= G151), Q137 (= Q152), E158 (= E173), H191 (= H206)
binding magnesium ion: S42 (≠ T57), Q81 (= Q96), D157 (= D172)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
42% identity, 76% coverage: 18:306/378 of query aligns to 3:296/371 of 3puxA

query
sites
3puxA

V

V

Q

Q

L

L

A

Q

G

N

I

V

R

T

K

K

C

A

F
x
W

D

G

G

E

K

V

E

V

V
|
V

I

S

P

K

Q

D

L

I

D

N

L

L

T

D

I

I

N

H

N

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

V

L

L

L

R

R

L

M

I

I

A

A

G

G

L

L

E

E

T

T

V

I

D

T

S

S

G

G

R

D

I

L

M

F

L

I

D

G

N

E

E

K

D

R

I

M

T

N

H

D

V

T

P

P

A

P

E

A

N

E

R

R

Y

G

V

V

N

G

T

M

V

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

F

A

E

E

N

N

V

M

A

S

F

F

G

G

L

L

R

K

M

L

Q

A

K

G

T

A

P

K

A

K

A

E

E

V

I

I

T

N

P

Q

R

R

V

V

M

N

E

Q

A

V

L

A

R

E

M

V

V

L

Q

Q

L

L

E

A

T

H

F

L

A

L

Q

D

R
|
R

K

K

P

P

H

K

Q
x
A

L
|
L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

N

A

K

E

P

P

R

S

L

V

L

F

L

L

D
|
D

E
|
E

S

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

A

L

L

R

R

K

V

Q

Q

M

M

Q

R

N

I

E

E

L

I

K

S

A

R

L

L

Q

H

R

K

K

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

I

I

V

V

M

L

R

D

D

A

G

G

R

R

I

V

E

A

Q

Q

D

V

G

G

T

K

P

P

R

L

E

E

I

L

Y

Y

E

H

E

Y

P

P

K

A

N

D

L

R

F

F

V

V

A

A

G

G

F

F

I

I

G

G

E

S

I

P

N

K

M

M

-

N

-

F

-

L

-

P

-

V

-

K

F

V

N

T

A

A

T

T

V

A

I

I

E

D

R

Q

L

V

-

Q

-

V

-

E

-

L

-

P

-

M

-

P

D

N

E

R

Q

Q

R

Q

V

V

R

W

A

L

N

P

V

V

E

E

G

S

R

R

E

D

C

V

N

Q

I

V

Y

G

V

A

N

N

F

M

A

S

V

L

E

G

P

-

G

-

Q

-

K

-

L

-

H

-

V

-

L

-

L

I

R

R

P

P

E

E

D

H

L

L

R

L

V

P

E

S

E

D

I

I

N

A

D

D

binding adenosine-5'-diphosphate: W12 (≠ F27), V17 (= V32), S37 (= S52), G38 (= G53), C39 (= C54), G40 (= G55), K41 (= K56), S42 (≠ T57), T43 (= T58), R128 (= R143), A132 (≠ Q147), L133 (= L148), S134 (= S149), Q137 (= Q152)
binding beryllium trifluoride ion: S37 (= S52), G38 (= G53), K41 (= K56), Q81 (= Q96), S134 (= S149), G135 (= G150), G136 (= G151), E158 (= E173), H191 (= H206)
binding magnesium ion: S42 (≠ T57), Q81 (= Q96), D157 (= D172)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
42% identity, 76% coverage: 18:306/378 of query aligns to 3:296/371 of 3puwA

query
sites
3puwA

V

V

Q

Q

L

L

A

Q

G

N

I

V

R

T

K

K

C

A

F
x
W

D

G

G

E

K

V

E

V

V
|
V

I

S

P

K

Q

D

L

I

D

N

L

L

T

D

I

I

N

H

N

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

V

L

L

L

R

R

L

M

I

I

A

A

G

G

L

L

E

E

T

T

V

I

D

T

S

S

G

G

R

D

I

L

M

F

L

I

D

G

N

E

E

K

D

R

I

M

T

N

H

D

V

T

P

P

A

P

E

A

N

E

R

R

Y

G

V

V

N

G

T

M

V

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

F

A

E

E

N

N

V

M

A

S

F

F

G

G

L

L

R

K

M

L

Q

A

K

G

T

A

P

K

A

K

A

E

E

V

I

I

T

N

P

Q

R

R

V

V

M

N

E

Q

A

V

L

A

R

E

M

V

V

L

Q

Q

L

L

E

A

T

H

F

L

A

L

Q

D

R
|
R

K

K

P

P

H

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

N

A

K

E

P

P

R

S

L

V

L

F

L

L

D

D

E
|
E

S

P

L

L

S

S

A
x
N

L

L

D

D

Y

A

K

A

L

L

R

R

K

V

Q

Q

M

M

Q

R

N

I

E

E

L

I

K

S

A

R

L

L

Q

H

R

K

K

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

I

I

V

V

M

L

R

D

D

A

G

G

R

R

I

V

E

A

Q

Q

D

V

G

G

T

K

P

P

R

L

E

E

I

L

Y

Y

E

H

E

Y

P

P

K

A

N

D

L

R

F

F

V

V

A

A

G

G

F

F

I

I

G

G

E

S

I

P

N

K

M

M

-

N

-

F

-

L

-

P

-

V

-

K

F

V

N

T

A

A

T

T

V

A

I

I

E

D

R

Q

L

V

-

Q

-

V

-

E

-

L

-

P

-

M

-

P

D

N

E

R

Q

Q

R

Q

V

V

R

W

A

L

N

P

V

V

E

E

G

S

R

R

E

D

C

V

N

Q

I

V

Y

G

V

A

N

N

F

M

A

S

V

L

E

G

P

-

G

-

Q

-

K

-

L

-

H

-

V

-

L

-

L

I

R

R

P

P

E

E

D

H

L

L

R

L

V

P

E

S

E

D

I

I

N

A

D

D

binding adenosine-5'-diphosphate: W12 (≠ F27), V17 (= V32), S37 (= S52), G38 (= G53), C39 (= C54), G40 (= G55), K41 (= K56), S42 (≠ T57), T43 (= T58), R128 (= R143), A132 (≠ Q147), S134 (= S149), Q137 (= Q152)
binding tetrafluoroaluminate ion: S37 (= S52), G38 (= G53), K41 (= K56), Q81 (= Q96), S134 (= S149), G135 (= G150), G136 (= G151), E158 (= E173), N162 (≠ A177), H191 (= H206)
binding magnesium ion: S42 (≠ T57), Q81 (= Q96)

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
42% identity, 76% coverage: 18:306/378 of query aligns to 3:296/374 of 2awnB

query
sites
2awnB

V

V

Q

Q

L

L

A

Q

G

N

I

V

R

T

K

K

C

A

F
x
W

D

G

G

E

K

V

E

V

V
|
V

I

S

P

K

Q

D

L

I

D

N

L

L

T

D

I

I

N

H

N

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

V

L

L

L

R

R

L

M

I

I

A

A

G

G

L

L

E

E

T

T

V

I

D

T

S

S

G

G

R

D

I

L

M

F

L

I

D

G

N

E

E

K

D

R

I

M

T

N

H

D

V

T

P

P

A

P

E

A

N

E

R

R

Y

G

V

V

N

G

T

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

F

A

E

E

N

N

V

M

A

S

F

F

G

G

L

L

R

K

M

L

Q

A

K

G

T

A

P

K

A

K

A

E

E

V

I

I

T

N

P

Q

R

R

V

V

M

N

E

Q

A

V

L

A

R

E

M

V

V

L

Q

Q

L

L

E

A

T

H

F

L

A

L

Q

D

R

R

K

K

P

P

H

K

Q

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

N

A

K

E

P

P

R

S

L

V

L

F

L

L

D

D

E

E

S

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

A

L

L

R

R

K

V

Q

Q

M

M

Q

R

N

I

E

E

L

I

K

S

A

R

L

L

Q

H

R

K

K

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

I

I

V

V

M

L

R

D

D

A

G

G

R

R

I

V

E

A

Q

Q

D

V

G

G

T

K

P

P

R

L

E

E

I

L

Y

Y

E

H

E

Y

P

P

K

A

N

D

L

R

F

F

V

V

A

A

G

G

F

F

I

I

G

G

E

S

I

P

N

K

M

M

-

N

-

F

-

L

-

P

-

V

-

K

F

V

N

T

A

A

T

T

V

A

I

I

E

D

R

Q

L

V

-

Q

-

V

-

E

-

L

-

P

-

M

-

P

D

N

E

R

Q

Q

R

Q

V

V

R

W

A

L

N

P

V

V

E

E

G

S

R

R

E

D

C

V

N

Q

I

V

Y

G

V

A

N

N

F

M

A

S

V

L

E

G

P

-

G

-

Q

-

K

-

L

-

H

-

V

-

L

-

L

I

R

R

P

P

E

E

D

H

L

L

R

L

V

P

E

S

E

D

I

I

N

A

D

D

binding adenosine-5'-diphosphate: W12 (≠ F27), V17 (= V32), S37 (= S52), G38 (= G53), C39 (= C54), G40 (= G55), K41 (= K56), S42 (≠ T57), T43 (= T58)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
42% identity, 76% coverage: 18:306/378 of query aligns to 4:297/371 of P68187

query
sites
P68187

V

V

Q

Q

L

L

A

Q

G

N

I

V

R

T

K

K

C

A

F

W

D

G

G

E

K

V

E

V

V

V

I

S

P

K

Q

D

L

I

D

N

L

L

T

D

I

I

N

H

N

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

V

L

L

L

R

R

L

M

I

I

A

A

G

G

L

L

E

E

T

T

V

I

D

T

S

S

G

G

R

D

I

L

M

F

L

I

D

G

N

E

E

K

D

R

I

M

T

N

H

D

V

T

P

P

A

P

E

A

N

E

R

R

Y

G

V

V

N

G

T

M

V

V

F

F

Q

Q

S

S

Y

Y

A
|
A

L

L

F

Y

P

P

H

H

M

L

T

S

V

V

F

A

E

E

N

N

V

M

A

S

F

F

G

G

L

L

R

K

M

L

Q

A

K

G

T

A

P
x
K

A

K

A

E

E

V

I

I

T

N

P

Q

R

R

V
|
V

M

N

E

Q

A
x
V

L

A

R
x
E

M

V

V

L

Q

Q

L

L

E
x
A

T

H

F

L

A

L

Q

D

R

R

K

K

P

P

H

K

Q

A

L

L

S

S

G

G

G
|
G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

N

A

K

E

P

P

R

S

L

V

L

F

L

L

D
|
D

E

E

S

P

L

L

S

S

A

N

L

L

D

D

Y

A

K

A

L

L

R

R

K

V

Q

Q

M

M

Q

R

N

I

E

E

L

I

K

S

A

R

L

L

Q

H

R

K

K

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

L

M

T

T

M

L

S

A

D

D

R

K

I

I

V

V

M

L

R

D

D

A

G

G

R

R

I

V

E

A

Q

Q

D

V

G

G

T

K

P

P

R

L

E

E

I

L

Y

Y

E

H

E

Y

P

P

K

A

N

D

L
x
R

F

F

V

V

A

A

G

G

F

F

I

I

G

G

E

S

I

P

N

K

M

M

-

N

-
x
F

-

L

-

P

-

V

-

K

F

V

N

T

A

A

T

T

V

A

I

I

E

D

R

Q

L

V

-

Q

-

V

-

E

-

L

-

P

-

M

-

P

D

N

E

R

Q

Q

R

Q

V

V

R
x
W

A

L

N

P

V

V

E

E

G

S

R

R

E

D

C

V

N

Q

I

V

Y
x
G

V

A

N

N

F

M

A
x
S

V

L

E
x
G

P

-

G

-

Q

-

K

-

L

-

H

-

V

-

L

-

L

I

R

R

P

P

E

E

D

H

L

L

R

L

V

P

E

S

E

D

I

I

N

A

D

D

A85 (= A99) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ P120) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V128) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ A131) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ R133) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ E138) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G151) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D172) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ L242) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
F241 (vs. gap) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
W267 (≠ R268) mutation to G: Normal maltose transport but constitutive mal gene expression.
G278 (≠ Y279) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
S282 (≠ A283) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G284 (≠ E285) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

3d31A Modbc from methanosarcina acetivorans (see paper)
43% identity, 70% coverage: 36:299/378 of query aligns to 19:276/348 of 3d31A

query
sites
3d31A

L

L

D

S

L

L

T

K

I

V

N

E

N

S

G

G

E

E

F

Y

L

F

T

V

L

I

L

L

G

G

P

P

S

T

G

G

C

A

G

G

K

K

T

T

T

L

V

F

L

L

R

E

L

L

I

I

A

A

G

G

L

F

E

H

T

V

V

P

D

D

S

S

G

G

R

R

I

I

M

L

L

L

D

D

N

G

E

K

D

D

I

V

T

T

H

D

V

L

P

S

A

P

E

E

N

K

R

H

Y

D

V

I

N

A

T

F

V

V

F

Y

Q

Q

S

N

Y

Y

A

S

L

L

F

F

P

P

H

H

M

M

T

N

V

V

F

K

E

K

N

N

V

L

A

E

F

F

G

G

L

M

R

R

M

M

Q

K

K

K

T

I

P

K

A

D

A

P

E

K

I

-

T

-

P

-

R

R

V

V

M

L

E

D

A

T

L

A

R

R

M

D

V

L

Q

K

L

I

E

E

T

H

F

L

A

L

Q

D

R

R

K

N

P

P

H

L

Q

T

L

L

S

S

G

G

Q

E

Q

Q

R

R

V

V

A

A

I

L

A

A

R

R

A

A

V

L

V

V

N

T

K

N

P

P

R

K

L

I

L

L

D

D

E

E

S

P

L

L

S

S

A

A

L

L

D

D

Y

P

K

R

L

T

R

Q

K

E

Q

N

M

A

Q

R

N

E

E

M

L

L

K

S

A

V

L

L

Q

H

R

K

K

K

L

N

G

K

I

L

T

T

F

V

V

L

F

H

V

I

T

T

H

H

D

D

Q

Q

E

T

E

E

A

A

L

R

T

I

M

M

S

A

D

D

R

R

I

I

V

A

V

V

M

V

R

M

D

D

G

G

R

K

I

L

E

I

Q

Q

D

V

G

G

T

K

P

P

R

E

E

E

I

I

Y

F

E

E

E

K

P

P

K

V

N

E

L

G

F

R

V

V

A

A

G

S

F

F

I

V

G

G

E

F

I

E

N

N

M

V

F

L

N

K

A

G

T

R

V

V

I

I

E

S

R

A

L

-

D

-

E

E

Q

Q

-

G

-

L

R

R

V

I

R

R

A

V

N

G

V

-

E

-

G

-

R

-

E

E

C

V

N

V

I

I

Y

D

V

A

N

A

F

G

A

D

V

M

E

E

P

V

G

G

Q

D

K

Q

L

V

H

Y

V

A

L

F

L

L

R

R

P

P

E

E

D

N

L

I

Sites not aligning to the query:

binding tungstate(vi)ion: 286, 287, 288, 319, 320, 323, 340, 341, 342

2awnC Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
41% identity, 72% coverage: 36:306/378 of query aligns to 14:266/344 of 2awnC

query
sites
2awnC

L

I

D

N

L

L

T

D

I

I

N

H

N

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

V

L

L

L

R

R

L

M

I

I

A

A

G

G

L

L

E

G

T

-

V

-

D

-

S

-

G

-

R

-

I

-

M

-

L

-

D

-

N

-

E

-

D

-

I

-

T

-

H

-

V

-

P

-

A

-

E

-

N

-

R

-

Y

-

V

V

N

G

T

M

V

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H