SitesBLAST
Comparing 15248 b1126 putrescine/spermidine ABC transporter ATPase protein (NCBI) to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
100% identity, 100% coverage: 1:378/378 of query aligns to 1:378/378 of P69874
- C26 (= C26) mutation to A: Lower ATPase activity and transport efficiency.
- F27 (= F27) mutation to L: Lower ATPase activity and transport efficiency.
- F45 (= F45) mutation to L: Lower ATPase activity and transport efficiency.
- C54 (= C54) mutation to T: Loss of ATPase activity and transport.
- L60 (= L60) mutation to F: Lower ATPase activity and transport efficiency.
- L76 (= L76) mutation to P: Lower ATPase activity and transport efficiency.
- V135 (= V135) mutation to M: Loss of ATPase activity and transport.
- D172 (= D172) mutation to N: Loss of ATPase activity and transport.
- C276 (= C276) mutation to A: Lower ATPase activity and transport efficiency.
- E297 (= E297) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.
2d62A Crystal structure of multiple sugar binding transport atp- binding protein
52% identity, 64% coverage: 18:260/378 of query aligns to 7:257/375 of 2d62A
1g291 Malk (see paper)
51% identity, 64% coverage: 18:260/378 of query aligns to 4:254/372 of 1g291
- binding magnesium ion: D69 (= D77), E71 (= E79), K72 (≠ D80), K79 (≠ E87), D80 (≠ N88)
- binding pyrophosphate 2-: S38 (= S52), G39 (= G53), C40 (= C54), G41 (= G55), K42 (= K56), T43 (= T57), T44 (= T58)
Sites not aligning to the query:
P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
53% identity, 61% coverage: 28:256/378 of query aligns to 15:245/393 of P9WQI3
- H193 (= H206) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.
8hprD Lpqy-sugabc in state 4 (see paper)
51% identity, 61% coverage: 28:257/378 of query aligns to 14:245/362 of 8hprD
- binding adenosine-5'-triphosphate: S38 (= S52), C40 (= C54), G41 (= G55), K42 (= K56), S43 (≠ T57), T44 (= T58), Q82 (= Q96), R129 (= R143), Q133 (= Q147), S135 (= S149), G136 (= G150), G137 (= G151), Q159 (≠ E173), H192 (= H206)
- binding magnesium ion: S43 (≠ T57), Q82 (= Q96)
Sites not aligning to the query:
8hprC Lpqy-sugabc in state 4 (see paper)
51% identity, 61% coverage: 28:257/378 of query aligns to 14:245/363 of 8hprC
- binding adenosine-5'-triphosphate: S38 (= S52), G39 (= G53), G41 (= G55), K42 (= K56), S43 (≠ T57), Q82 (= Q96), Q133 (= Q147), G136 (= G150), G137 (= G151), Q138 (= Q152), H192 (= H206)
- binding magnesium ion: S43 (≠ T57), Q82 (= Q96)
Sites not aligning to the query:
8hplC Lpqy-sugabc in state 1 (see paper)
51% identity, 60% coverage: 30:257/378 of query aligns to 14:243/384 of 8hplC
Sites not aligning to the query:
1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
46% identity, 79% coverage: 18:317/378 of query aligns to 7:295/353 of 1vciA
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
43% identity, 76% coverage: 18:306/378 of query aligns to 4:295/369 of P19566
- L86 (= L100) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (≠ S174) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D179) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Sites not aligning to the query:
- 306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
42% identity, 76% coverage: 18:306/378 of query aligns to 1:294/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ F27), S35 (= S52), G36 (= G53), C37 (= C54), G38 (= G55), K39 (= K56), S40 (≠ T57), T41 (= T58), R126 (= R143), A130 (≠ Q147), S132 (= S149), G134 (= G151), Q135 (= Q152)
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
42% identity, 76% coverage: 18:306/378 of query aligns to 3:296/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F27), S37 (= S52), G38 (= G53), C39 (= C54), G40 (= G55), K41 (= K56), S42 (≠ T57), T43 (= T58), Q81 (= Q96), R128 (= R143), A132 (≠ Q147), S134 (= S149), G136 (= G151), Q137 (= Q152), E158 (= E173), H191 (= H206)
- binding magnesium ion: S42 (≠ T57), Q81 (= Q96)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
42% identity, 76% coverage: 18:306/378 of query aligns to 3:296/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ F27), G38 (= G53), C39 (= C54), G40 (= G55), K41 (= K56), S42 (≠ T57), T43 (= T58), R128 (= R143), S134 (= S149), Q137 (= Q152)
- binding beryllium trifluoride ion: S37 (= S52), G38 (= G53), K41 (= K56), Q81 (= Q96), S134 (= S149), G136 (= G151), H191 (= H206)
- binding magnesium ion: S42 (≠ T57), Q81 (= Q96)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
42% identity, 76% coverage: 18:306/378 of query aligns to 3:296/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ F27), V17 (= V32), G38 (= G53), C39 (= C54), G40 (= G55), K41 (= K56), S42 (≠ T57), T43 (= T58), R128 (= R143), A132 (≠ Q147), S134 (= S149), Q137 (= Q152)
- binding tetrafluoroaluminate ion: S37 (= S52), G38 (= G53), K41 (= K56), Q81 (= Q96), S134 (= S149), G135 (= G150), G136 (= G151), E158 (= E173), H191 (= H206)
- binding magnesium ion: S42 (≠ T57), Q81 (= Q96)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
42% identity, 76% coverage: 18:306/378 of query aligns to 3:296/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ F27), V17 (= V32), G38 (= G53), C39 (= C54), G40 (= G55), K41 (= K56), S42 (≠ T57), T43 (= T58), R128 (= R143), A132 (≠ Q147), S134 (= S149), Q137 (= Q152)
- binding magnesium ion: S42 (≠ T57), Q81 (= Q96)
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
42% identity, 76% coverage: 18:306/378 of query aligns to 3:296/374 of 2awnB
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
42% identity, 76% coverage: 18:306/378 of query aligns to 4:297/371 of P68187
- A85 (= A99) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ P120) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (= V128) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ A131) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ R133) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ E138) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G151) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D172) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (≠ L242) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- F241 (vs. gap) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- W267 (≠ R268) mutation to G: Normal maltose transport but constitutive mal gene expression.
- G278 (≠ Y279) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- S282 (≠ A283) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G284 (≠ E285) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
Sites not aligning to the query:
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
3d31A Modbc from methanosarcina acetivorans (see paper)
43% identity, 70% coverage: 36:299/378 of query aligns to 19:276/348 of 3d31A
Sites not aligning to the query:
2awnC Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
41% identity, 72% coverage: 36:306/378 of query aligns to 14:266/344 of 2awnC
Sites not aligning to the query:
1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
36% identity, 80% coverage: 23:324/378 of query aligns to 9:308/353 of 1oxvD
1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
36% identity, 80% coverage: 23:324/378 of query aligns to 9:308/353 of 1oxvA
Query Sequence
>15248 b1126 putrescine/spermidine ABC transporter ATPase protein (NCBI)
MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGEFLTLLGPSGCGKTTVL
RLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFPHMTVFENVAFGLRMQKTP
AAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIARAVVNKPRLLLLDESLSALDY
KLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRIVVMRDGRIEQDGTPREIYEEPK
NLFVAGFIGEINMFNATVIERLDEQRVRANVEGRECNIYVNFAVEPGQKLHVLLRPEDLR
VEEINDDNHAEGLIGYVRERNYKGMTLESVVELENGKMVMVSEFFNEDDPDFDHSLDQKM
AINWVESWEVVLADEEHK
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory