SitesBLAST

Q8GAI3 4-methylaminobutanoate oxidase (formaldehyde-forming); MABO; Demethylating gamma-N-methylaminobutyrate oxidase; Gamma-N-methylaminobutyrate oxidase 1; EC 1.5.3.19 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
24% identity, 96% coverage: 1:416/432 of query aligns to 26:401/824 of Q8GAI3

query
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Q8GAI3

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W66 (= W55) mutation W->F,S: Contains a non-covalently bound FAD. Loss of enzyme activity.
H67 (≠ A56) mutation to A: Contains a non-covalently bound FAD. Exhibits about 10% of the wild-type enzyme activity.

1y56B Crystal structure of l-proline dehydrogenase from p.Horikoshii (see paper)
24% identity, 72% coverage: 109:418/432 of query aligns to 60:371/374 of 1y56B

query
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1y56B

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active site: H224 (≠ G274), P239 (= P288), G305 (= G353), M338 (≠ T385)
binding flavin-adenine dinucleotide: E170 (≠ P223), V171 (= V224), T200 (≠ F253), N201 (≠ G254), W203 (≠ Y256), G305 (= G353), Y306 (≠ L354), Y307 (≠ R355), G334 (≠ T381), H335 (≠ L382), G336 (= G383), F337 (≠ W384), M338 (≠ T385)
binding flavin mononucleotide: I260 (≠ G311), R301 (≠ T349), W303 (= W351)

Sites not aligning to the query:

active site: 44, 47, 48
binding flavin-adenine dinucleotide: 11, 13, 14, 15, 33, 34, 35, 42, 43, 45, 46, 47, 49
binding flavin mononucleotide: 44, 45

4yshB Crystal structure of glycine oxidase from geobacillus kaustophilus
25% identity, 71% coverage: 108:415/432 of query aligns to 61:368/368 of 4yshB

query
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4yshB

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active site: I262 (≠ V310), L283 (= L338), G305 (= G353), N335 (≠ L382), L338 (≠ T385)
binding flavin-adenine dinucleotide: V178 (= V224), S206 (≠ F253), W209 (≠ Y256), R307 (= R355), H332 (= H379), R334 (≠ T381), N335 (≠ L382), G336 (= G383), I337 (≠ W384), L338 (≠ T385)
binding glycine: G249 (≠ Y297), Y251 (≠ I299), Y251 (≠ I299), A264 (≠ G312), R307 (= R355), R334 (≠ T381), R334 (≠ T381)

Sites not aligning to the query:

active site: 45, 48, 49, 52
binding flavin-adenine dinucleotide: 9, 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 48

S5FMM4 Glycine oxidase; GO; BliGO; EC 1.4.3.19 from Bacillus licheniformis (see paper)
24% identity, 69% coverage: 103:400/432 of query aligns to 57:348/369 of S5FMM4

query
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S5FMM4

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S

L

I

L

E

C

S

D

E

G

A

E

G

Q

A

I

V

Y

R

G

-

V

V

K

T

C

S

G

A

D

S

E

G

V

T

I

A

K

E

A

A

D

E

A

H

Y

A

V

V

M

I

A

A

F
x
S

G

G

S

V

Y

W

S

S

T

G

A

A

M

L

L

F

K

K

G

Q

I

I

-

G

V

L

D

D

I

K

P

R

V

F

Y

Y

P

P

L

V

K

K

G

G

Y

E

S

C

L

L

T

S

I

V

P

-

I

-

A

-

Q

W

E

N

D

D

G

G

A

I

P

S

V

L

S

T

T

R

I

T

L

L

D

Y

E

H

T

D

Y

H

K

C

I

Y

A

I

I

V

T

P

R

R

F

H

D

S

N

G

R

R

I

L

R

V

V

V

G

G

G

A

M

T

A

M

E

K

I

P

V

G

G

D

F

W

N

N

T

E

E

-

L

-

Q

Q

P

P

R

E

R

L

E

G

T

G

L

I

E

E

M

E

V

L

V

I

R

R

-

K

-

A

-

K

D

S

L

M

Y

L

P

P

R

G

G

I

G

E

H

S

V

M

E

K

Q

I

A

D

T

Q

F

C

W

W

T

A

G

G

L

L

R

R

P

P

M

E

T

T

P

G

D

D

G

G

T

N

P

P

V

Y

V

I

G

G

R

R

T

H

-

P

R

E

F

N

K

D

N

R

L

I

W

L

L

F

N

A

T

A

G

G

H

H

G

F

T

R

L

N

G

G

W
x
I

T

L

M

L

A

A

C

P

G

A

S

T

G

G

Q

E

L

M

L
x
M

S

A

D

D

L

M

L

I

S

L

G

G

K81 (vs. gap) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.
S202 (≠ F253) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
I332 (≠ W384) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
M342 (≠ L394) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.

Sites not aligning to the query:

51 G→S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
54 A→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.

4yshA Crystal structure of glycine oxidase from geobacillus kaustophilus
25% identity, 71% coverage: 108:415/432 of query aligns to 61:368/370 of 4yshA

query
sites
4yshA

M

L

V

V

R

P

L

L

A

A

E

L

Y

Q

S

S

R

R

D

A

C

L

L

M

K

P

A

A

L

L

-

A

-

E

-

L

R

R

A

E

E

R

T

T

N

G

I

I

Q

D

Y

I

E

G

G

L

R

V

Q

E

G

K

G

G

T

L

L

I

Q

K

L

L

F

A

R

T

T

T

E

E

Q

E

Y

A

E

D

N

D

A

L

T

Y

R

R

D

H

I

Y

A

T

V

F

L

W

E

R

D

G

A

I

G

G

V

E

P

P

Y

V

Q

Q

L

W

L

L

E

T

S

K

S

G

R

E

L

A

A

L

E

E

V

M

E

E

P

P

A

R

L

L

A

A

E

E

V

A

A

-

H

-

K

-

L

L

T

A

G

G

G

A

L

M

Q

Y

L

I

P

P

N

G

D

D

E

G

T

Q

G

V

D

S

C

A

Q

P

L

D

F

L

T

A

Q

A

N

A

L

L

A

A

R

Y

M

A

A

A

E

A

Q

S

A

A

G

G

V

A

K

C

F

L

R

Y

F

E

N

Y

T

T

P

E

V
|
V

D

F

Q

D

L

I

L

R

C

S

D

D

G

S

E

S

Q

G

I

-

Y

H

G

V

V

L

K

D

C

T

G

T

D

G

E

G

V

T

I

F

K

A

A

A

D

E

A

A

Y

V

V

V

M

I

A

A

F
x
S

G
|
G

S

A

Y
x
W

S

A

T

A

A

R

M

L

L

G

K

A

G

R

I

V

-

G

V

L

D

S

I

L

P

S

V

V

Y

Y

P

P

L

V

K

K

G

G

Y

E

S

C

L

V

T

M

-

V

-

R

-

A

-

P

I

V

P

P

I

L

A

L

Q

Q

E

-

D

-

G

-

A

-

P

-

V

-

S

T

T

T

I

V

L

F

D

A

E

K

T

N

Y

G

K

C

I

Y

A

I

I

V

T

P

R

K

F

S

D

G

N

N

R

R

I

L

R

L

V
x
I

G

G

G

A

M

T

A

S

E

T

I

P

V

G

G

T

F

F

N

D

T

-

E

-

L

-

Q

-

P

-

R

-

R

R

E

R

T

V

L

S

E

A

M

G

V

G

V

V

R

M

D

N

L
|
L

Y

L

P

H

R

R

G

A

H

H

-

L

-

V

-

P

-

D

V

I

E

E

Q

Q

A

A

T

E

F

W

-

V

-

A

-

S

W

W

T

S

G
|
G

L

I

R
|
R

P

P

M

Q

T

T

P

E

D

D

G

G

T

L

P

P

V

Y

V

L

G

G

-

E

R

H

T

P

R

E

F

R

K

R

N

G

L

L

W

F

L

V

N

A

T

A

G

G

H
|
H

G

Y

T
x
R

L
x
N

G
|
G

W
x
I

T
x
L

M

L

A

S

C

P

G

L

S

T

G

G

Q

L

L

L

L

V

S

A

D

D

L

L

L

V

S

E

G

R

R

K

T

E

P

T

A

A

I

F

P

D

Y

L

E

A

D

P

L

F

S

S

V

L

A

T

R

R

Y

H

active site: I262 (≠ V310), L283 (= L338), G305 (= G353), N335 (≠ L382), L338 (≠ T385)
binding flavin-adenine dinucleotide: V178 (= V224), S206 (≠ F253), G207 (= G254), W209 (≠ Y256), R307 (= R355), H332 (= H379), R334 (≠ T381), N335 (≠ L382), G336 (= G383), I337 (≠ W384)

Sites not aligning to the query:

active site: 45, 48, 49, 52
binding flavin-adenine dinucleotide: 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 47, 48

Q5L2C2 Glycine oxidase; GO; GOX; GOXK; EC 1.4.3.19 from Geobacillus kaustophilus (strain HTA426)
25% identity, 71% coverage: 108:415/432 of query aligns to 62:370/377 of Q5L2C2

query
sites
Q5L2C2

M

L

V

V

R

P

L

L

A

A

E

L

Y

Q

S

S

R

R

D

A

C

L

L

M

K

P

A

A

L

L

-

A

-

E

-

L

R

R

A

E

E

R

T

T

N

G

I

I

Q

D

Y

I

E

G

G

L

R

V

Q

E

G

K

G

G

T

L

L

I

Q

K

L

L

F

A

R

T

T

T

E

E

Q

E

Y

A

E

D

N

D

A

L

T

Y

R

R

D

H

I

Y

A

T

V

F

L

W

E

R

D

G

A

I

G

G

V

E

P

P

Y

V

Q

Q

L

W

L

L

E

T

S

K

S

G

R

E

L

A

A

L

E

E

V

M

E

E

P

P

A

R

L

L

A

A

E

-

V

-

A

A

H

E

K

A

L

L

T

A

G

G

G

A

L

M

Q

Y

L

I

P

P

N

G

D

D

E

G

T

Q

G

V

D

S

C

A

Q

P

L

D

F

L

T

A

Q

A

N

A

L

L

A

A

R

Y

M

A

A

A

E

A

Q

S

A

A

G

G

V

A

K

C

F

L

R

Y

F

E

N

Y

T

T

P

E

V
|
V

D

F

Q

D

L

I

L

R

C

S

D

D

G

S

E

S

Q

G

I

-

Y

H

G

V

V

L

K

D

C

T

G

T

D

G

E

G

V

T

I

F

K

A

A

A

D

E

A

A

Y

V

V

V

M

I

A

A

F

S

G

G

S

A

Y

W

S

A

T

A

A

R

M

L

L

G

K

A

G

R

I

V

-

G

V

L

D

S

I

L

P

S

V

V

Y

Y

P

P

L

V

K

K

G

G

Y

E

S

C

L

V

T

M

-

V

-

R

-

A

-

P

I

V

P

P

I

L

A

L

Q

Q

E

-

D

-

G

-

A

-

P

-

V

-

S

T

T

T

I

V

L

F

D

A

E

K

T

N

Y

G

K

C

I

Y

A

I

I

V

T

P

R

K

F

S

D

G

N

N

R

R

I

L

R

L

V

I

G

G

G

A

M

T

A

S

E

T

I

P

V

G

G

T

F

F

N

D

T

-

E

-

L

-

Q

-

P

-

R

-

R

R

E

R

T

V

L

S

E

A

M

G

V

G

V

V

R

M

D

N

L

L

Y

L

P

H

R

R

G

A

H

H

-

L

-

V

-

P

-

D

V

I

E

E

Q

Q

A

A

T

E

F

W

-

V

-

A

-

S

W

W

T

S

G

G

L

I

R
|
R

P

P

M

Q

T

T

P

E

D

D

G

G

T

L

P

P

V

Y

V

L

G

G

-

E

R

H

T

P

R

E

F

R

K

R

N

G

L

L

W

F

L

V

N

A

T

A

G

G

H
|
H

G
x
Y

T
x
R

L
x
N

G
|
G

W
x
I

T
x
L

M

L

A

S

C

P

G

L

S

T

G

G

Q

L

L

L

L

V

S

A

D

D

L

L

L

V

S

E

G

R

R

K

T

E

P

T

A

A

I

F

P

D

Y

L

E

A

D

P

L

F

S

S

V

L

A

T

R

R

Y

H

V180 (= V224) binding
R309 (= R355) binding
334:340 (vs. 379:385, 29% identical) binding
R336 (≠ T381) binding

Sites not aligning to the query:

14:15 binding
34:35 binding
42:43 binding
47:49 binding

2gagB Heteroteterameric sarcosine: structure of a diflavin metaloenzyme at 1.85 a resolution (see paper)
22% identity, 66% coverage: 134:418/432 of query aligns to 102:390/403 of 2gagB

query
sites
2gagB

Q

Q

G

R

G

G

T

V

L

L

Q

N

L

L

F

A

R

H

T

T

E

L

Q

G

Q

D

Y

V

E

R

N

E

A

S

T

V

R

R

D

R

I

V

A

E

V

A

L

N

E

K

D

L

A

N

G

G

V

V

P

D

Y

A

Q

E

L

W

L

L

E

D

S

P

S

S

R

Q

L

V

A

K

E

E

V

A

E

C

P

P

A

I

L

I

A

N

-

T

-

S

-

D

E

D

V

I

A

R

H

Y

K

P

L

V

T

M

G

G

G

A

L

T

Q

W

L

Q

P

P

N

R

D

A

E

G

T

I

G

A

D
x
K

C
x
H

Q

D

L

H

F

V

T

A

Q

W

N

A

L

F

A

A

R

R

M

K

A

A

E

N

Q

E

A

M

G

G

V

V

K

D

F

I

R

I

F

Q

N

N

T

C

P

E

V
|
V

D

T

Q

G

L

F

L

I

C

K

D

D

G

G

E

E

Q

K

I

V

Y

T

G

G

V

V

K

K

C

T

G

T

D

R

E

G

V

T

I

I

K

H

A

A

D

G

A

K

Y

V

V

A

M

L

A

A

F
x
G

G
x
A

S

G

Y
x
H

S

S

T

S

A

V

M
x
L

L

A

K

E

G

-

I

-

V

-

D

-

I

-

P

-

V

-

Y

-

P

-

L

M

K

A

G

G

Y

F

S

E

L

L

T

P

I

I

P

Q

I

-

A

S

Q

H

E

P

D
x
L

G

Q

A

A

P

L

V
|
V

S

S

T

E

I

L

L

F

D

E

E

P

T

V

Y

H

K

P

I

T

A

V

I

V

T

-

R

-

F
x
M

D

S

N

N

R

H

I

I

R

H

V

V

-
x
Y

-

V

-

S

-

Q

-

A

-

H

-
x
K

G

G

G
x
E

M

L

A

V

E

M

I

G

V

A

G

G

F

I

N

D

T

S

E

Y

L

N

L

G

Q

Y

P

G

R

Q

R

R

E

G

T

A

L

F

E

H

M

V

V

I

V

Q

R

E

-

Q

-

M

-

A

-

V

D

E

L

L

Y

F

P

P

R

I

G

F

G

A

H

R

V

A

E

H

Q

V

A

L

T
x
R

F

T

W
|
W

T

G

G

G

L

I

R

V

P

D

M

T

T

T

P

M

D

D

G

A

T

S

P

P

V

I

G

S

R

K

T

T

R

P

F

I

K

Q

N

N

L

L

W

Y

L

V

N

N

T

C

G

G

H

W

G
|
G

T
|
T

L
x
G

G
|
G

W
x
F

T
x
K

M

G

A

T

C

P

G

G

S

A

G

G

Q

F

L

T

L

L

S

A

D

H

L

T

L

I

S

A

G

N

R

D

T

E

P

P

A

H

I

E

P

L

Y

N

E

K

D

P

L

F

S

S

V

L

A

E

R

R

Y

F

S

E

R

T

G

G

binding flavin-adenine dinucleotide: V195 (= V224), G224 (≠ F253), A225 (≠ G254), H227 (≠ Y256), L231 (≠ M260), L246 (≠ D285), G352 (= G380), T353 (= T381), G354 (≠ L382), G355 (= G383), F356 (≠ W384), K357 (≠ T385)
binding flavin mononucleotide: H171 (≠ C200), V250 (= V289), E278 (≠ G312), R321 (≠ T349), W323 (= W351)
binding 2-furoic acid: M263 (≠ F304), Y270 (vs. gap), K357 (≠ T385)
binding sulfite ion: K170 (≠ D199), K276 (vs. gap)

Sites not aligning to the query:

active site: 61, 64, 65
binding flavin-adenine dinucleotide: 26, 28, 29, 30, 51, 52, 58, 59, 60, 62, 63, 64, 66
binding flavin mononucleotide: 61, 62
binding 2-furoic acid: 64, 66, 68, 401

P40875 Sarcosine oxidase subunit beta; Sarcosine oxidase subunit B; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta; Tetrameric sarcosine oxidase subunit beta; TSOX subunit beta; EC 1.5.3.24 from Corynebacterium sp. (strain P-1) (see 3 papers)
22% identity, 74% coverage: 111:431/432 of query aligns to 81:405/405 of P40875

query
sites
P40875

L

I

A

Y

E

E

Y

K

S

S

R

L

D

K

C

L

L

W

K

E

A

Q

L

L

R

P

A

E

E

E

T

L

N

D

I

Y

Q

D

Y

F

E

L

G

F

R

S

Q

Q

G

R

G

G

T

V

L

L

Q

N

L

L

F

A

R

H

T

T

E

L

Q

G

Q

D

Y

V

E

R

N

E

A

S

T

V

R

R

D

R

I

V

A

E

V

A

L

N

E

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D

F

A

N

G

G

V

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P

D

Y

A

Q

E

L

W

L

L

E

T

S

P

S

E

R

Q

L

V

A

K

E

E

V

V

E
x
C

P

P

A

I

L

I

A

N

-

I

-

G

-

D

E

D

V

I

A

R

H

Y

K

P

L

V

T

M

G

G

G

A

L

T

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Y

L

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P

P

N

R

D

A

E

G

T

I

G

A

D

K

C
x
H

Q

D

L
x
H

F

V

T

A

Q

W

N

A

L

F

A

A

R

R

M

K

A

A

E

N

Q

E

A

M

G

G

V

V

K

D

F

I

R

I

F

Q

N

N

T
x
C

P

E

V

V

D

T

Q

G

L

F

L

L

C

K

D

D

G

G

E

E

Q

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T

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C

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T

I

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H

A

A

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A

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G

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E

G

-

I

-

V

-

D

-

I

-

P

-

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-

Y

-

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-

L

L

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A

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G

Y

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-

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H

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P

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V

T

-

R

-

F

M

D

S

N

N

R

H

I

I

R

H

V

V

-

Y

-

V

-

S

-

Q

-

A

-

H

-

K

G

G

G

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M

L

A

V

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M

I

G

V

A

G

G

F

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P

G

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Q

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R

E

-

Q

-

M

-

A

-

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E

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P

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I

G

F

G

A

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T

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W

T

G

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D

D

G

A

T

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P

P

V

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G

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R

K

T

T

R

P

F

I

K

Q

N

N

L

L

W

Y

L

V

N

N

T
x
C

G

G

H

W

G

G

T

T

L

G

G

G

W

F

T

K

M

G

A

T

C

P

G

G

S

A

G

G

Q

F

L

T

L

L

S

A

D

H

L

T

L

I

S

A

G

N

R

D

T

E

P

A

A

H

I

A

P

L

Y

N

E

A

D

P

L

F

S

S

V

L

A

E

R

R

Y

F

S

E

R

T

G

G

F

H

T

L

P

I

S

D

R

E

P

H

G

G

H

A

L

A

H

A

G

V

A

A

H

H

C146 (≠ E176) mutation to S: No change in activity.
H173 (≠ C200) modified: Tele-8alpha-FMN histidine; mutation to N: Prevents covalent attachment of FMN. Loss of activity. The mutant is considerably less stable than wild-type enzyme, the beta and delta subunits are lost during purification, which yields a stable alpha-gamma complex.
H175 (≠ L202) mutation to A: No effect on FMN binding and activity.
C195 (≠ T222) mutation to S: No change in activity.
C351 (≠ T377) mutation to A: No change in activity.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
30 G→A: Prevents covalent attachment of FMN. Blocks subunit assembly.

6j39A Crystal structure of cmis2 with inhibitor (see paper)
25% identity, 91% coverage: 11:405/432 of query aligns to 11:359/368 of 6j39A

query
sites
6j39A

V
x
I

G

G

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A

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Y

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T

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x
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D
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D

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x
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E
|
E

P

P

G

-

A

-

L

-

E

-

T

-

S

-

A

A

A

S

N
x
K

A
|
A

G
x
S

Q

H

I
x
V

S
|
S

P
x
A

G
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G

Y
x
M

A

-

P

-

W

-

A

-

P

-

G

-

V

-

P

-

L

-

K

-

A

-

I

-

K

-

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-

M

-

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-

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-

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-

H

-

A

-

P

-

L

-

A

-

V

-

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-

L

L

D
x
P

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A

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A

Q
x
N

F

E

Q

M

L

L

K

Y

W

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M

Q

W

E

Q

D

M

L

L

L

R

R

N

L

C

C

D

L

T

A

S

S

H

R

Y

-

M

-

E

-

N

-

K

-

G

-

R

-

M

-

V

-

R

-

L

-

A

-

E

E

Y

R

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Y

R

P

D

S

C

F

L

V

K

K

A

E

L

L

R

E

A

A

E

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T

S

N

G

I

T

Q

S

Y

A

E

G

G

Y

R

R

Q

R

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D

G

G

T

V

L

L

Q

D

L

A

F

A

R

F

T

D

E

D

Q

E

Q

S

Y

L

E

A

N

A

A

L

T

D

R

G

D

L

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N

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A

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P

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L

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S

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R

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L

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L

A

A

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E

V

-

A

-

H

-

K

S

L

V

T

R

G

G

L

L

Q

L

L

G

P

P

N

D

D

D

E

G

T

A

G

V

D

N

C

P

Q

R

L

E

F

L

T

T

Q

A

N

A

L

L

-

L

A

A

R

A

M

I

A

D

E

V

Q

R

A

G

G

G

V

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K

L

F

I

R

R

F

R

N

R

T
x
A

P

-

V

-

D

T

Q

E

L

F

L

L

C

A

D

D

G

-

E

E

Q

R

I

T

Y

P

G

G

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L

C

L

G

E

D

N

E

G

-

C

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I

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K

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A

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D

D

A

R

Y

V

V

V

M

L

A

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F
x
A

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S

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Y
x
W

S

-

T

T

A

H

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R

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A

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D

A

-

G

-

A

I

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P

-

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I

Y

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P

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A

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K

G

G

Y

Q

S
x
I

L

L

T

R

I

L

P

-

I

-

A

-

Q

-

E

R

D

S

G

A

A

A

P

P

-

F

-

L

-

R

-

A

V

T

S
x
R

T

A

I

V

L

T

D

R

E

G

T

S

Y

G

K

V

I
x
Y

A

L

I

V

T

P

R

R

F

T

D

D

N

G

R

E

I

L

R

V

V

V

G

G

G

A

M

T

A
x
Y

E

E

I

E

V

R

G

D

F

Y

N

D

T

T

E

T

L

V

L

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Q

A

P

G

R

G

R

V

E

A

T

E

L

L

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L

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G

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K

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V

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L

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A

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V

Y

L

P

P

R

G

G

A

H

E

V

L

E

E

Q

L

A

A

T

E

F

T

W

A

T

A

G

G

L

L

R
|
R

P

P

M

G

T

S

P

P

D

D

G

G

T

L

P

P

V

V

V

L

G

G

R

W

T

T

R

A

F

V

K

P

N

N

L

L

W

L

L

V

N

A

T

T

G

G

H

H

G
x
S

T
x
R

L
x
I

G
|
G

W
x
V

T
x
Q

M

L

A

A

C

P

G

I

S

T

G

A

Q

D

L

V

L

M

S

G

D

E

L

M

L

L

-

V

S

T

G

G

R

R

T

T

P

P

A

E

I

V

binding (3R)-3-[(carboxymethyl)sulfanyl]nonanoic acid: M44 (≠ Y51), P46 (≠ D80), N49 (≠ Q83), R243 (≠ S290), Y252 (≠ I299), Y267 (≠ A314), R308 (= R355), R334 (≠ T381), I335 (≠ L382)
binding flavin-adenine dinucleotide: I11 (≠ V11), V29 (≠ I29), D30 (= D30), P31 (≠ R31), E32 (= E32), K36 (≠ N43), A37 (= A44), S38 (≠ G45), V40 (≠ I47), S41 (= S48), A42 (≠ P49), G43 (= G50), M44 (≠ Y51), A174 (≠ T222), A203 (≠ F253), W206 (≠ Y256), I228 (≠ S276), Y252 (≠ I299), R308 (= R355), S333 (≠ G380), R334 (≠ T381), I335 (≠ L382), G336 (= G383), V337 (≠ W384), Q338 (≠ T385)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 7, 9, 10

6j38A Crystal structure of cmis2 (see paper)
25% identity, 91% coverage: 11:405/432 of query aligns to 11:359/368 of 6j38A

query
sites
6j38A

V
x
I

G

G

V

L

A

S

S

V

A

A

W

W

Y

Q

L

A

N

L

Q

E

A

R

G

G

H

L

E

R

V

V

T

T

V

V

I
x
V

D
|
D

R
x
P

E

E

P

P

G

-

A

-

L

-

E

-

T

-

S

-

A

A

A

S

N
x
K

A
|
A

G
x
S

Q

H

I

V

S
|
S

P
x
A

G
|
G

Y
x
M

A

-

P

-

W

-

A

-

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-

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-

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-

P

-

L

-

K

-

A

-

I

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K

-

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-

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Q

-

R

-

H

-

A

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-

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-

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-

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L

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A

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A

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W

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Q

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M

L

L

L

R

R

N

L

C

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A

S

S

H

R

Y

-

M

-

E

-

N

-

K

-

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-

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-

M

-

V

-

R

-

L

-

A

-

E

E

Y

R

S

Y

R

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D

S

C

F

L

V

K

K

A

E

L

L

R

E

A

A

E

V

T

S

N

G

I

T

Q

S

Y

A

E

G

G

Y

R

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Q

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D

L

A

F

A

R

F

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D

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D

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L

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A

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A

A

L

T

D

R

G

D

L

I

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A

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D

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A

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A

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A

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R

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C

A

R

E

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A

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-

A

-

H

-

K

S

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G

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L

L

G

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P

N

D

D

D

E

G

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A

G

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C

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Q

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L

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F

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T

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A

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L

A

A

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A

M

I

A

D

E

V

Q

R

A

G

G

G

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F

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N

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T
x
A

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-

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A

D

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E

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E

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D

A

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Y

V

V

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M

L

A

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F
x
A

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x
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S

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A

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A

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G

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A

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K

G
|
G

Y

Q

S

I

L

L

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I

L

P

-

I

-

A

-

Q

-

E

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D

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A

A

A

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-

F

-

L

-

R

-

A

V

T

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R

T

A

I

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E

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T

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Y

G

K

V

I

Y

A

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I

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R

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G

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E

I

L

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V

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V

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G

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A

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T

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E

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D

T

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V

E

A

T

E

L

L

E

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A

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A

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T

E

F

T

W

A

T

A

G
|
G

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R
|
R

P

P

M

G

T

S

P

P

D

D

G

G

T

L

P

P

V

V

V

L

G

G

R

W

T

T

R

A

F

V

K

P

N

N

L

L

W

L

L

V

N

A

T

T

G

G

H

H

G
x
S

T
x
R

L
x
I

G
|
G

W
x
V

T
x
Q

M

L

A

A

C

P

G

I

S

T

G

A

Q

D

L

V

L

M

S

G

D

E

L

M

L

L

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V

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G

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P

A

E

I

V

binding flavin-adenine dinucleotide: I11 (≠ V11), V29 (≠ I29), D30 (= D30), P31 (≠ R31), K36 (≠ N43), A37 (= A44), S38 (≠ G45), S41 (= S48), A42 (≠ P49), G43 (= G50), M44 (≠ Y51), A174 (≠ T222), A203 (≠ F253), W206 (≠ Y256), G226 (= G274), G306 (= G353), R308 (= R355), S333 (≠ G380), R334 (≠ T381), I335 (≠ L382), G336 (= G383), V337 (≠ W384), Q338 (≠ T385)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 7, 9

Q50LF2 Sarcosine oxidase subunit beta; Sarcosine oxidase subunit B; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta; Tetrameric sarcosine oxidase subunit beta; TSOX subunit beta; EC 1.5.3.24 from Corynebacterium sp. (strain U-96) (see 4 papers)
22% identity, 66% coverage: 134:418/432 of query aligns to 104:392/405 of Q50LF2

query
sites
Q50LF2

Q

Q

G

R

G

G

T

V

L

L

Q

N

L

L

F

A

R

H

T

T

E

L

Q

G

Q

D

Y

V

E

R

N

E

A

S

T

I

R

R

D

R

I

V

A

E

V

A

L

N

E

K

D

F

A

N

G

G

V

V

P

D

Y

A

Q

E

L

W

L

L

E

T

S

P

S

E

R

Q

L

V

A

K

E

E

V

V

E

C

P

P

A

I

L

I

A

N

-

T

-

G

-

D

E

N

V

I

A

R

H

Y

K

P

L

V

T

M

G

G

G

A

L

T

Q

Y

L

Q

P

P

N

R

D

A

E

G

T

I

G

A

D
x
K

C
x
H

Q

D

L

H

F

V

T

A

Q

W

N

A

L

F

A

A

R

R

M

K

A

A

E

N

Q

E

A

M

G

G

V

V

K

D

F

I

R

I

F

Q

N

N

T

C

P

E

V
|
V

D

T

Q

G

L

F

L

L

C

K

D

D

G

G

E

E

Q

K

I

V

Y

T

G

G

V

V

K

K

C

T

G

T

D

R

E

G

V

T

I

I

K

L

A

A

D

G

A

K

Y

V

V

A

M

L

A

A

F

G

G

A

S

G

Y

H

S

S

T

S

A

V

M

L

L

A

K

E

G

-

I

-

V

-

D

-

I

-

P

-

V

-

Y

-

P

-

L

L

K

A

G

G

Y

F

S

E

L

L

T

P

I

I

P

Q

I

-

A

S

Q

H

E

P

D

L

G

Q

A

A

P

L

V

V

S

S

T

E

I

L

L

F

D

E

E

P

T

V

Y

H

K

P

I

T

A

V

I

V

T

-

R

-

F

M

D

S

N

N

R

H

I

I

R
x
H

V

V

-
x
Y

-

V

-

S

-

Q

-

A

-

H

-

K

G

G

G

E

M

L

A

V

E

M

I

G

V

A

G

G

F

I

N

D

T

S

E

Y

L

N

L

G

Q

Y

P

G

R

Q

R

R

E

G

T

A

L

F

E

H

M

V

V

I

V

E

R

E

-

Q

-

M

-

A

-

V

D

E

L

L

Y

F

P

P

R

I

G

F

G

A

H

R

V

A

E

H

Q

V

A

L

T

R

F

T

W

W

T

G

G

G

L

I

R

V

P

D

M

T

T

T

P

M

D

D

G

A

T

S

P

P

V

I

G

S

R

K

T

T

R

P

F

I

K

Q

N

N

L

L

W

Y

L

V

N

N

T

C

G

G

H

W

G
|
G

T

T

L

G

G
|
G

W

F

T
x
K

M

G

A

T

C

P

G

G

S

A

G

G

Q

Y

L

T

L

L

S

A

D

H

L

T

L

I

S

A

G

H

R

D

T

E

P

P

A

H

I

K

P

L

Y

N

E

A

D

P

L

F

S

A

V

L

A

E

R

R

Y

F

S

E

R

T

G

G

K172 (≠ D199) mutation to A: Retains 39% of wild-type activity.; mutation to D: Retains 32% of wild-type activity.; mutation to R: Retains 58% of wild-type activity.
H173 (≠ C200) modified: Tele-8alpha-FMN histidine
V197 (= V224) binding
H270 (≠ R309) mutation to A: 10-fold decrease in catalytic efficiency.
Y272 (vs. gap) mutation to A: 13000-fold decrease in catalytic efficiency.; mutation to F: 130-fold decrease in catalytic efficiency.
G354 (= G380) binding
G357 (= G383) binding
K359 (≠ T385) binding ; mutation K->A,D: Loss of activity.; mutation to R: Retains 0.07% of wild-type activity. Shows higher apparent KM for sarcosine.

Sites not aligning to the query:

31 binding
32 binding
53 binding
61 binding
62 binding
66 binding
68 binding

Q9AGP8 Dimethylglycine oxidase; DMGO; EC 1.5.3.10 from Arthrobacter globiformis (see 2 papers)
24% identity, 61% coverage: 108:369/432 of query aligns to 62:349/830 of Q9AGP8

query
sites
Q9AGP8

M

M

V

A

R

S

L

F

A

A

E

K

Y

Y

S

T

R

V

D

E

C

K

L

L

K

L

A

S

L

L

R

-

A

T

E

E

T

D

N

G

I

V

Q

S

Y

C

E

F

G

N

R

Q

Q

V

G

G

T

-

L

L

Q

E

L

V

F

A

R

T

T

T

E

E

Q

T

Q

R

Y

L

E

A

N

D

A

L

T

K

R

R

D

K

I

L

A

G

V

Y

L

A

E

A

D

A

A

W

G

G

V

I

P

E

Y

G

Q

R

L

L

E

S

S

P

S

A

R

E

L

C

A

Q

E

E

V

L

E

Y

P

P

A

L

L

L

A

D

E

-

V

-

A

G

H

E

K

N

L

I

T

L

G

G

L

L

Q

H

L

V

P

P

N

S

D

D

E

G

T

L

G

A

D

S

C

A

Q

A

L

R

F

A

T

V

Q

Q

N

L

L

L

A

I

R

K

M

R

A

T

E

E

Q

S

A

A

G

G

V

V

K

T

F

Y

R

R

F

G

N

S

T

T

P

T

V
|
V

D

T

Q

G

L

I

L

E

C

Q

D

S

G

G

E

G

Q

R

I

V

Y

T

G

G

V

V

K

Q

C

T

G

A

D

D

E

G

V

V

I

I

K

P

A

A

D

D

A

I

Y

V

V

V

M

S

A

C

F

A

G

G

S

F

Y

W

S

G

T

A

A

K

M

I

L

G

K

A

G

M

I

I

-

G

V

M

D

A

I

V

P

P

V

L

Y

L

P

P

L

L

-

A

K
x
H

G

Q

Y

Y

S

V

L

K

T

T

I

T

P

P

I

V

A

P

-

A

-

Q

-

G

-

R

-

N

-

D

Q

Q

E

P

D

N

G

G

A

A

P

R

V

L

S

P

T

I

I

L

L

R

D

H

E

Q

T

D

Y

Q

K

D

I

L

A
x
Y

I

Y

T

R

R

E

F

H

D

G

N

D

R

R

I

Y

R

G

V

I

G

G

G

S

M

Y

A

A

E

H

-

R

-

P

-

M

I

P

V

V

G

D

F

V

N

D

T

T

-

L

-

G

-

A

-

Y

-

A

-

P

-

E

-

T

-

V

-

S

E

E

L

H

Q

M

P

P

R

S

R

R

E

L

T

D

L

F

E

T

M

L

-

E

-

D

-

F

-

L

-

P

-

A

-

W

-

E

V

A

V

T

R

K

D

Q

L

L

Y

L

P

P

R

A

G

L

G

A

H

D

V

S

E

E

Q

I

A

E

T

D

F

G

W

F

T

N

G

G

L

I

R

F

P

S

M

F

T

T

P

P

D

D

G

G

T

G

P

P

V

L

G

G

R

E

T

S

R

K

V174 (= V224) binding
H225 (≠ K273) Important for catalytic activity; mutation to Q: Reduces catalytic efficiency 3-fold and substrate affinity 30-fold.
Y259 (≠ A300) Important for catalytic activity; binding ; mutation to F: Reduces catalytic efficiency 225-fold and substrate affinity 25-fold.

Sites not aligning to the query:

14:15 binding
35:36 binding
45:48 binding
52 binding
360:363 binding
539 binding
552 Important for catalytic activity; D→A: No effect on the activity.; D→N: Reduces activity 3-fold.

1vrqB Crystal structure of heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with folinic acid (see paper)
22% identity, 66% coverage: 134:418/432 of query aligns to 103:391/402 of 1vrqB

query
sites
1vrqB

Q

Q

G

R

G

G

T

V

L

L

Q

N

L

L

F

A

R

H

T

T

E

L

Q

G

Q

D

Y

V

E

R

N

E

A

S

T

I

R

R

D

R

I

V

A

E

V

A

L

N

E

K

D

F

A

N

G

G

V

V

P

D

Y

A

Q

E

L

W

L

L

E

T

S

P

S

E

R

Q

L

V

A

K

E

E

V

V

E

C

P

P

A

I

L

I

A

N

-

T

-

G

-

D

E

N

V

I

A

R

H

Y

K

P

L

V

T

M

G

G

G

A

L

T

Q

Y

L

Q

P

P

N

R

D

A

E

G

T

I

G

A

D

K

C
x
H

Q

D

L

H

F

V

T

A

Q

W

N

A

L

F

A

A

R

R

M

K

A

A

E

N

Q

E

A

M

G

G

V

V

K

D

F

I

R

I

F

Q

N

N

T

C

P

E

V
|
V

D

T

Q

G

L

F

L

L

C

K

D

D

G

G

E

E

Q

K

I

V

Y

T

G

G

V

V

K

K

C

T

G

T

D

R

E

G

V

T

I

I

K

L

A

A

D

G

A

K

Y

V

V

A

M

L

A
|
A

F
x
G

G

A

S

G

Y
x
H

S

S

T

S

A

V

M

L

L

A

K

E

G

-

I

-

V

-

D

-

I

-

P

-

V

-

Y

-

P

-

L

L

K

A

G

G

Y

F

S

E

L

L

T

P

I

I

P

Q

I

-

A

S

Q

H

E

P

D
x
L

G

Q

A

A

P

L

V
|
V

S

S

T

E

I

L

L

F

D

E

E

P

T

V

Y

H

K

P

I

T

A

V

I

V

T

-

R

-

F

M

D

S

N

N

R

H

I

I

R

H

V

V

-

Y

-

V

-

S

-

Q

-

A

-

H

-

K

G

G

G
x
E

M

L

A

V

E

M

I

G

V

A

G

G

F

I

N

D

T

S

E

Y

L

N

L

G

Q

Y

P

G

R

Q

R

R

E

G

T

A

L

F

E

H

M

V

V

I

V

E

R

E

-

Q

-

M

-

A

-

V

D

E

L

L

Y

F

P

P

R

I

G

F

G

A

H

R

V

A

E

H

Q

V

A

L

T
x
R

F

T

W
|
W

T

G

G
|
G

L

I

R

V

P

D

M

T

T

T

P

M

D

D

G

A

T

S

P

P

V

I

G

S

R

K

T

T

R

P

F

I

K

Q

N

N

L

L

W

Y

L

V

N

N

T

C

G

G

H

W

G
|
G

T
|
T

L
x
G

G
|
G

W
x
F

T
x
K

M

G

A

T

C

P

G

G

S

A

G

G

Q

Y

L

T

L

L

S

A

D

H

L

T

L

I

S

A

G

H

R

D

T

E

P

P

A

H

I

K

P

L

Y

N

E

A

D

P

L

F

S

A

V

L

A

E

R

R

Y

F

S

E

R

T

G

G

active site: G326 (= G353), K358 (≠ T385)
binding n,n-dimethylglycine: K358 (≠ T385)
binding flavin-adenine dinucleotide: V196 (= V224), A224 (= A252), G225 (≠ F253), H228 (≠ Y256), L247 (≠ D285), G353 (= G380), T354 (= T381), G355 (≠ L382), G356 (= G383), F357 (≠ W384), K358 (≠ T385)
binding flavin mononucleotide: H172 (≠ C200), V251 (= V289), E279 (≠ G312), R322 (≠ T349), W324 (= W351)

Sites not aligning to the query:

active site: 62, 65, 66
binding n,n-dimethylglycine: 65, 67, 69, 401
binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 51, 52, 53, 59, 60, 61, 63, 64, 65, 67
binding flavin mononucleotide: 62, 63

3ad8B Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with pyrrole 2-carboxylate (see paper)
22% identity, 66% coverage: 134:418/432 of query aligns to 103:391/404 of 3ad8B

query
sites
3ad8B

Q

Q

G

R

G

G

T

V

L

L

Q

N

L

L

F

A

R

H

T

T

E

L

Q

G

Q

D

Y

V

E

R

N

E

A

S

T

I

R

R

D

R

I

V

A

E

V

A

L

N

E

K

D

F

A

N

G

G

V

V

P

D

Y

A

Q

E

L

W

L

L

E

T

S

P

S

E

R

Q

L

V

A

K

E

E

V

V

E

C

P

P

A

I

L

I

A

N

-

T

-

G

-

D

E

N

V

I

A

R

H

Y

K

P

L

V

T

M

G

G

G

A

L

T

Q

Y

L

Q

P

P

N

R

D

A

E

G

T

I

G

A

D

K

C
x
H

Q

D

L

H

F

V

T

A

Q

W

N

A

L

F

A

A

R

R

M

K

A

A

E

N

Q

E

A

M

G

G

V

V

K

D

F

I

R

I

F

Q

N

N

T

C

P

E

V
|
V

D

T

Q

G

L

F

L

L

C

K

D

D

G

G

E

E

Q

K

I

V

Y

T

G

G

V

V

K

K

C

T

G

T

D

R

E

G

V

T

I

I

K

L

A

A

D

G

A

K

Y

V

V

A

M

L

A

A

F
x
G

G
x
A

S

G

Y
x
H

S

S

T

S

A

V

M

L

L

A

K

E

G

-

I

-

V

-

D

-

I

-

P

-

V

-

Y

-

P

-

L

L

K

A

G

G

Y

F

S

E

L

L

T

P

I

I

P

Q

I

-

A

S

Q

H

E

P

D
x
L

G

Q

A

A

P

L

V
|
V

S

S

T

E

I

L

L

F

D

E

E

P

T

V

Y

H

K

P

I

T

A

V

I

V

T

-

R

-

F
x
M

D

S

N

N

R

H

I

I

R

H

V

V

-
x
Y

-

V

-

S

-

Q

-

A

-

H

-

K

G

G

G
x
E

M

L

A

V

E

M

I

G

V

A

G

G

F

I

N

D

T

S

E

Y

L

N

L

G

Q

Y

P

G

R

Q

R

R

E

G

T

A

L

F

E

H

M

V

V

I

V

E

R

E

-

Q

-

M

-

A

-

V

D

E

L

L

Y

F

P

P

R

I

G

F

G

A

H

R

V

A

E

H

Q

V

A

L

T
x
R

F

T

W
|
W

T

G

G
|
G

L

I

R

V

P

D

M

T

T

T

P

M

D

D

G

A

T

S

P

P

V

I

G

S

R

K

T

T

R

P

F

I

K

Q

N

N

L

L

W

Y

L

V

N

N

T

C

G

G

H

W

G
|
G

T
|
T

L
x
G

G
|
G

W
x
F

T
x
K

M

G

A

T

C

P

G

G

S

A

G

G

Q

Y

L

T

L

L

S

A

D

H

L

T

L

I

S

A

G

H

R

D

T

E

P

P

A

H

I

K

P

L

Y

N

E

A

D

P

L

F

S

A

V

L

A

E

R

R

Y

F

S

E

R

T

G

G

active site: G326 (= G353), K358 (≠ T385)
binding flavin-adenine dinucleotide: V196 (= V224), G225 (≠ F253), A226 (≠ G254), H228 (≠ Y256), L247 (≠ D285), G353 (= G380), T354 (= T381), G355 (≠ L382), G356 (= G383), F357 (≠ W384), K358 (≠ T385)
binding flavin mononucleotide: H172 (≠ C200), V251 (= V289), E279 (≠ G312), R322 (≠ T349), W324 (= W351)
binding pyrrole-2-carboxylate: M264 (≠ F304), Y271 (vs. gap), T354 (= T381), K358 (≠ T385)

Sites not aligning to the query:

active site: 62, 65, 66
binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 52, 53, 59, 60, 61, 63, 64, 65, 67
binding flavin mononucleotide: 62, 63
binding pyrrole-2-carboxylate: 65, 67, 69, 402

3ad7B Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with methylthio acetate (see paper)
22% identity, 66% coverage: 134:418/432 of query aligns to 103:391/404 of 3ad7B

query
sites
3ad7B

Q

Q

G

R

G

G

T

V

L

L

Q

N

L

L

F

A

R

H

T

T

E

L

Q

G

Q

D

Y

V

E

R

N

E

A

S

T

I

R

R

D

R

I

V

A

E

V

A

L

N

E

K

D

F

A

N

G

G

V

V

P

D

Y

A

Q

E

L

W

L

L

E

T

S

P

S

E

R

Q

L

V

A

K

E

E

V

V

E

C

P

P

A

I

L

I

A

N

-

T

-

G

-

D

E

N

V

I

A

R

H

Y

K

P

L

V

T

M

G

G

G

A

L

T

Q

Y

L

Q

P

P

N

R

D

A

E

G

T

I

G

A

D

K

C
x
H

Q

D

L

H

F

V

T

A

Q

W

N

A

L

F

A

A

R

R

M

K

A

A

E

N

Q

E

A

M

G

G

V

V

K

D

F

I

R

I

F

Q

N

N

T

C

P

E

V
|
V

D

T

Q

G

L

F

L

L

C

K

D

D

G

G

E

E

Q

K

I

V

Y

T

G

G

V

V

K

K

C

T

G

T

D

R

E

G

V

T

I

I

K

L

A

A

D

G

A

K

Y

V

V

A

M

L

A

A

F
x
G

G
x
A

S

G

Y
x
H

S

S

T

S

A

V

M

L

L

A

K

E

G

-

I

-

V

-

D

-

I

-

P

-

V

-

Y

-

P

-

L

L

K

A

G

G

Y

F

S

E

L

L

T

P

I

I

P

Q

I

-

A

S

Q

H

E

P

D
x
L

G

Q

A

A

P

L

V
|
V

S

S

T

E

I

L

L

F

D

E

E

P

T

V

Y

H

K

P

I

T

A

V

I

V

T

-

R

-

F
x
M

D

S

N

N

R

H

I

I

R

H

V

V

-
x
Y

-

V

-

S

-

Q

-

A

-

H

-
x
K

G

G

G
x
E

M

L

A

V

E

M

I

G

V

A

G

G

F

I

N

D

T

S

E

Y

L

N

L

G

Q

Y

P

G

R

Q

R

R

E

G

T

A

L

F

E

H

M

V

V

I

V

E

R

E

-

Q

-

M

-

A

-

V

D

E

L

L

Y

F

P

P

R

I

G

F

G

A

H

R

V

A

E

H

Q

V

A

L

T
x
R

F

T

W
|
W

T

G

G
|
G

L

I

R

V

P

D

M

T

T

T

P

M

D

D

G

A

T

S

P

P

V

I

G

S

R

K

T

T

R

P

F

I

K

Q

N

N

L

L

W

Y

L

V

N

N

T

C

G

G

H

W

G
|
G

T
|
T

L
x
G

G
|
G

W
x
F

T
x
K

M

G

A

T

C

P

G

G

S

A

G

G

Q

Y

L

T

L

L

S

A

D

H

L

T

L

I

S

A

G

H

R

D

T

E

P

P

A

H

I

K

P

L

Y

N

E

A

D

P

L

F

S

A

V

L

A

E

R

R

Y

F

S

E

R

T

G

G

active site: G326 (= G353), K358 (≠ T385)
binding flavin-adenine dinucleotide: V196 (= V224), G225 (≠ F253), A226 (≠ G254), H228 (≠ Y256), L247 (≠ D285), G353 (= G380), T354 (= T381), G355 (≠ L382), G356 (= G383), F357 (≠ W384), K358 (≠ T385)
binding flavin mononucleotide: H172 (≠ C200), V251 (= V289), K277 (vs. gap), E279 (≠ G312), R322 (≠ T349), W324 (= W351)
binding [methylthio]acetate: M264 (≠ F304), Y271 (vs. gap), T354 (= T381), K358 (≠ T385)

Sites not aligning to the query:

active site: 62, 65, 66
binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 52, 53, 59, 60, 61, 63, 64, 65, 67
binding flavin mononucleotide: 62, 63
binding [methylthio]acetate: 67, 69, 402

1pj6A Crystal structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folic acid (see paper)
24% identity, 61% coverage: 108:369/432 of query aligns to 60:347/828 of 1pj6A

query
sites
1pj6A

M

M

V

A

R

S

L

F

A

A

E

K

Y

Y

S

T

R

V

D

E

C

K

L

L

K

L

A

S

L

L

R

-

A

T

E

E

T

D

N

G

I

V

Q

S

Y

C

E

F

G

N

R

Q

Q

V

G

G

T

-

L

L

Q

E

L

V

F

A

R

T

T

T

E

E

Q

T

Q

R

Y

L

E

A

N

D

A

L

T

K

R

R

D

K

I

L

A

G

V

Y

L

A

E

A

D

A

A

W

G

G

V

I

P

E

Y

G

Q

R

L

L

E

S

S

P

S

A

R

E

L

C

A

Q

E

E

V

L

E

Y

P

P

A

L

L

L

A

D

E

-

V

-

A

G

H

E

K

N

L

I

T

L

G

G

L

L

Q

H

L

V

P

P

N

S

D

D

E

G

T

L

G

A

D

S

C

A

Q

A

L

R

F

A

T

V

Q

Q

N

L

L

L

A

I

R

K

M

R

A

T

E

E

Q

S

A

A

G

G

V

V

K

T

F

Y

R

R

F

G

N

S

T

T

P

T

V
|
V

D

T

Q

G

L

I

L

E

C

Q

D

S

G

G

E

G

Q

R

I

V

Y

T

G

G

V

V

K

Q

C

T

G

A

D

D

E

G

V

V

I

I

K

P

A

A

D

D

A

I

Y

V

V

V

M

S

A

C

F
x
A

G
|
G

S

F

Y
x
W

S

G

T

A

A

K

M

I

L

G

K

A

G

M

I

I

-

G

V

M

D

A

I

V

P

P

V

L

Y

L

P

P

L

L

-

A

K
x
H

G

Q

Y

Y

S

V

L

K

T

T

I

T

P

P

I

V

A

P

-

A

-

Q

-

G

-

R

-

N

-

D

Q

Q

E

P

D

N

G

G

A

A

P

R

V

L

S

P

T

I

I

L

L

R

D

H

E

Q

T

D

Y

Q

K

D

I

L

A
x
Y

I

Y

T

R

R

E

F

H

D

G

N

D

R

R

I

Y

R

G

V

I

G

G

G

S

M

Y

A

A

E

H

-

R

-

P

-

M

I

P

V

V

G

D

F

V

N

D

T

T

-

L

-

G

-

A

-

Y

-

A

-

P

-

E

-

T

-

V

-

S

E

E

L

H

Q

M

P

P

R

S

R

R

E

L

T

D

L

F

E

T

M

L

-

E

-

D

-

F

-

L

-

P

-

A

-

W

-

E

V

A

V

T

R

K

D

Q

L

L

Y

L

P

P

R

A

G

L

G

A

H

D

V

S

E

E

Q

I

A

E

T

D

F

G

W

F

T

N

G
|
G

L
x
I

R

F

P

S

M

F

T

T

P

P

D

D

G

G

T

G

P

P

V

L

G

G

R

E

T

S

R

K

active site: H223 (≠ K273), Y257 (≠ A300)
binding flavin-adenine dinucleotide: V172 (= V224), A201 (≠ F253), G202 (= G254), W204 (≠ Y256), H223 (≠ K273), Y257 (≠ A300), G331 (= G353), I332 (≠ L354)

Sites not aligning to the query:

active site: 550
binding flavin-adenine dinucleotide: 9, 11, 12, 13, 33, 34, 42, 43, 44, 46, 48, 50, 358, 359, 360, 361

3gsiA Crystal structure of d552a dimethylglycine oxidase mutant of arthrobacter globiformis in complex with tetrahydrofolate (see paper)
24% identity, 61% coverage: 108:369/432 of query aligns to 59:346/827 of 3gsiA

query
sites
3gsiA

M

M

V

A

R

S

L

F

A

A

E

K

Y

Y

S

T

R

V

D

E

C

K

L

L

K

L

A

S

L

L

R

-

A

T

E

E

T

D

N

G

I

V

Q

S

Y

C

E

F

G

N

R

Q

Q

V

G

G

T

-

L

L

Q

E

L

V

F

A

R

T

T

T

E

E

Q

T

Q

R

Y

L

E

A

N

D

A

L

T

K

R

R

D

K

I

L

A

G

V

Y

L

A

E

A

D

A

A

W

G

G

V

I

P

E

Y

G

Q

R

L

L

E

S

S

P

S

A

R

E

L

C

A

Q

E

E

V

L

E

Y

P

P

A

L

L

L

A

D

E

-

V

-

A

G

H

E

K

N

L

I

T

L

G

G

L

L

Q

H

L

V

P

P

N

S

D

D

E

G

T

L

G

A

D

S

C

A

Q

A

L

R

F

A

T

V

Q

Q

N

L

L

L

A

I

R

K

M

R

A

T

E

E

Q

S

A

A

G

G

V

V

K

T

F

Y

R

R

F

G

N

S

T

T

P
x
T

V
|
V

D

T

Q

G

L

I

L

E

C

Q

D

S

G

G

E

G

Q

R

I

V

Y

T

G

G

V

V

K

Q

C

T

G

A

D

D

E

G

V

V

I

I

K

P

A

A

D

D

A

I

Y

V

V

V

M

S

A

C

F
x
A

G
|
G

S

F

Y
x
W

S

G

T

A

A

K

M

I

L

G

K

A

G

M

I

I

-

G

V

M

D

A

I

V

P

P

V

L

Y

L

P

P

L

L

-

A

K
x
H

G

Q

Y

Y

S

V

L

K

T

T

I

T

P

P

I

V

A

P

-

A

-

Q

-

G

-

R

-

N

-

D

Q

Q

E

P

D

N

G

G

A

A

P

R

V

L

S

P

T

I

I

L

L

R

D

H

E

Q

T

D

Y

Q

K
x
D

I

L

A
x
Y

I

Y

T

R

R

E

F

H

D

G

N

D

R

R

I

Y

R

G

V

I

G

G

G

S

M

Y

A

A

E

H

-

R

-

P

-

M

I

P

V

V

G

D

F

V

N

D

T

T

-

L

-

G

-

A

-

Y

-

A

-

P

-

E

-

T

-

V

-

S

E

E

L

H

Q

M

P

P

R

S

R

R

E

L

T

D

L

F

E

T

M

L

-

E

-

D

-

F

-

L

-

P

-

A

-

W

-

E

V

A

V

T

R

K

D

Q

L

L

Y

L

P

P

R

A

G

L

G

A

H

D

V

S

E

E

Q

I

A

E

T

D

F

G

W

F

T

N

G
|
G

L
x
I

R
x
F

P

S

M

F

T

T

P

P

D

D

G

G

T

G

P

P

V

L

G

G

R

E

T

S

R

K

active site: H222 (≠ K273), Y256 (≠ A300)
binding flavin-adenine dinucleotide: T170 (≠ P223), V171 (= V224), A200 (≠ F253), G201 (= G254), W203 (≠ Y256), H222 (≠ K273), Y256 (≠ A300), G330 (= G353), I331 (≠ L354), F332 (≠ R355)
binding magnesium ion: D254 (≠ K298)

Sites not aligning to the query:

active site: 549
binding flavin-adenine dinucleotide: 10, 11, 12, 32, 33, 41, 42, 43, 45, 47, 49, 357, 358, 359, 360
binding magnesium ion: 409
binding (6s)-5,6,7,8-tetrahydrofolate: 505, 536, 551, 563, 629, 648, 655, 696

1pj7A Structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folinic acid (see paper)
24% identity, 61% coverage: 108:369/432 of query aligns to 59:346/827 of 1pj7A

query
sites
1pj7A

M

M

V

A

R

S

L

F

A

A

E

K

Y

Y

S

T

R

V

D

E

C

K

L

L

K

L

A

S

L

L

R

-

A

T

E

E

T

D

N

G

I

V

Q

S

Y

C

E

F

G

N

R

Q

Q

V

G

G

T

-

L

L

Q

E

L

V

F

A

R

T

T

T

E

E

Q

T

Q

R

Y

L

E

A

N

D

A

L

T

K

R

R

D

K

I

L

A

G

V

Y

L

A

E

A

D

A

A

W

G

G

V

I

P

E

Y

G

Q

R

L

L

E

S

S

P

S

A

R

E

L

C

A

Q

E

E

V

L

E

Y

P

P

A

L

L

L

A

D

E

-

V

-

A

G

H

E

K

N

L

I

T

L

G

G

L

L

Q

H

L

V

P

P

N

S

D

D

E

G

T

L

G

A

D

S

C

A

Q

A

L

R

F

A

T

V

Q

Q

N

L

L

L

A

I

R

K

M

R

A

T

E

E

Q

S

A

A

G

G

V

V

K

T

F

Y

R

R

F

G

N

S

T

T

P
x
T

V
|
V

D

T

Q

G

L

I

L

E

C

Q

D

S

G

G

E

G

Q

R

I

V

Y

T

G

G

V

V

K

Q

C

T

G

A

D

D

E

G

V

V

I

I

K

P

A

A

D

D

A

I

Y

V

V

V

M

S

A

C

F
x
A

G
|
G

S

F

Y
x
W

S

G

T

A

A

K

M

I

L

G

K

A

G

M

I

I

-

G

V

M

D

A

I

V

P

P

V

L

Y

L

P

P

L

L

-

A

K
x
H

G

Q

Y

Y

S

V

L

K

T

T

I

T

P

P

I

V

A

P

-

A

-

Q

-

G

-

R

-

N

-

D

Q

Q

E

P

D

N

G

G

A

A

P

R

V

L

S

P

T

I

I

L

L

R

D

H

E

Q

T

D

Y

Q

K

D

I

L

A
x
Y

I

Y

T

R

R

E

F

H

D

G

N

D

R

R

I

Y

R

G

V

I

G

G

G

S

M

Y

A

A

E

H

-

R

-

P

-

M

I

P

V

V

G

D

F

V

N

D

T

T

-

L

-

G

-

A

-

Y

-

A

-

P

-

E

-

T

-

V

-

S

E

E

L

H

Q

M

P

P

R

S

R

R

E

L

T

D

L

F

E

T

M

L

-

E

-

D

-

F

-

L

-

P

-

A

-

W

-

E

V

A

V

T

R

K

D

Q

L

L

Y

L

P

P

R

A

G

L

G

A

H

D

V

S

E

E

Q

I

A

E

T

D

F

G

W

F

T

N

G

G

L
x
I

R

F

P

S

M

F

T

T

P

P

D

D

G

G

T

G

P

P

V

L

G

G

R

E

T

S

R

K

active site: H222 (≠ K273), Y256 (≠ A300)
binding flavin-adenine dinucleotide: T170 (≠ P223), V171 (= V224), A200 (≠ F253), G201 (= G254), W203 (≠ Y256), H222 (≠ K273), Y256 (≠ A300), I331 (≠ L354)

Sites not aligning to the query:

active site: 549
binding flavin-adenine dinucleotide: 8, 10, 11, 12, 32, 33, 41, 42, 43, 45, 47, 49, 357, 358, 359, 360
binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: 505, 536, 549, 551, 563, 629, 648, 655, 696

O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
25% identity, 45% coverage: 209:401/432 of query aligns to 159:349/369 of O31616

query
sites
O31616

R

K

M

A

A

A

E

K

Q

M

A

L

G

G

V

A

K

E

F

I

R

F

F

E

N

H

T

T

P

P

V
|
V

D

L

Q

H

L

V

L

E

C

R

D

D

G

G

E

E

Q

A

I

L

Y

F

G

-

V

I

K

K

C

T

G

P

D

S

E

G

V

D

I

V

K

W

A

A

D

N

A

H

Y

V

V

V

M

V

A

A

F

S

G

G

S

V

Y

W

S

S

T

G

A

M

M

F

L

F

K

K

G

Q

I

L

-

G

V

L

D

N

I

N

P

A

V

F

Y

L

P

P

L

V

K

K

G

G

Y

E

S

C

L

L

T

S

I

V

P

-

I

-

A

-

Q

W

E

N

D

D

G

D

A

I

P

P

V

L

S

T

T

K

I

T

L

L

D

Y

E

H

T

D

Y
x
H

K

C

I

Y

A

I

I

V

T

P

R

R

F

K

D

S

N

G

R

R

I

L

R

V

V

V

G

G

G

A

M

T

A

M

E

K

I

P

V

G

G

D

F

W

N

S

T

E

E

T

L

P

L

D

Q

L

P

G

R

G

R

L

E

E

T

S

L

V

E

M

M

K

V

K

V

A

R

K

D

T

L

M

Y

L

P

P

R

A

G

I

G

Q

H

N

V

M

E

K

Q

V

A

D

T

R

F

F

W

W

T

A

G

G

L

L

R
|
R

P

P

M

G

T

T

P

K

D

D

G

G

T

K

P

P

V

Y

V

I

G

G

R

R

T

H

-

P

R

E

F

D

K

S

N

R

L

I

W

L

L

F

N

A

T

A

G

G

H
|
H

G
x
F

T
x
R

L
x
N

G
|
G

W
x
I

T
x
L

M

L

A

A

C

P

G

A

S

T

G

G

Q

A

L

L

L

I

S

S

D

D

L

L

L

I

S

M

G

N

R

K

V174 (= V224) binding
H244 (≠ Y297) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
R302 (= R355) binding
327:333 (vs. 379:385, 29% identical) binding
R329 (≠ T381) binding

Sites not aligning to the query:

14:15 binding
34:35 binding
42:43 binding
47:49 binding
51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.

Query Sequence

>15311 FitnessBrowser__Keio:15311
MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANAGQISPGYAAPWAAPGV
PLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYMENKGRMVRLAEYSRDCLK
ALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDAGVPYQLLESSRLAEVEPALA
EVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKFRFNTPVDQLLCDGEQIYGVKCG
DEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYSLTIPIAQEDGAPVSTILDETYKIA
ITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMVVRDLYPRGGHVEQATFWTGLRPMTPD
GTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQLLSDLLSGRTPAIPYEDLSVARYSRGFT
PSRPGHLHGAHS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory