SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 15383 FitnessBrowser__Keio:15383 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1kgzB Crystal structure analysis of the anthranilate phosphoribosyltransferase from erwinia carotovora (current name, pectobacterium carotovorum) (see paper)
75% identity, 63% coverage: 199:530/531 of query aligns to 1:330/330 of 1kgzB

query
sites
1kgzB

T

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binding manganese (ii) ion: S92 (= S290), D222 (= D422), E223 (= E423), E223 (= E423)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V79 (= V277), G82 (= G280), G83 (= G281), N90 (= N288), I91 (= I289), S92 (= S290), T93 (= T291), K108 (= K306), S118 (= S318)

1i7qB Anthranilate synthase from s. Marcescens (see paper)
82% identity, 36% coverage: 2:193/531 of query aligns to 1:192/192 of 1i7qB

query
sites
1i7qB

A

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active site: G58 (= G59), C83 (= C84), L84 (= L85), H169 (= H170), E171 (= E172)
binding glutamic acid: P55 (= P56), G56 (= G57), G58 (= G59), C83 (= C84), L84 (= L85), Q87 (= Q88), H132 (= H133), S133 (= S134), L134 (= L135)

P00900 Anthranilate synthase component 2; AS; ASII; Anthranilate synthase, GATase component; Anthranilate synthase, glutamine amidotransferase component; EC 4.1.3.27 from Serratia marcescens (see 3 papers)
81% identity, 36% coverage: 1:193/531 of query aligns to 1:193/193 of P00900

query
sites
P00900

M
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M1 (= M1) modified: Initiator methionine, Removed
C84 (= C84) active site, Nucleophile; for GATase activity

5nofA Anthranilate phosphoribosyltransferase from thermococcus kodakaraensis (see paper)
39% identity, 60% coverage: 203:518/531 of query aligns to 3:311/325 of 5nofA

query
sites
5nofA

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binding zinc ion: E48 (≠ N250), D78 (= D282), E198 (= E403), D217 (= D422), E218 (= E423)

4yi7A Anthranilate bound at active site of anthranilate phosphoribosyl transferase from acinetobacter (anprt; trpd)
41% identity, 48% coverage: 200:452/531 of query aligns to 4:243/331 of 4yi7A

query
sites
4yi7A

L

I

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L

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A

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V

I

S

S

L

L

H

A

A

S

P

Q

T

T

I

Y

V

I

A

A

E

E

L

L

H

K

D

N

G

G

E

E

I

V

K

T

S

E

Y

W

Q

V

L

L

T

N

A

P

E

E

D

D

F

V

G

N

L

I

active site: V82 (= V277)
binding 2-aminobenzoic acid: V82 (= V277), G83 (= G278), H110 (= H307), R154 (= R364), G167 (= G377)

1zxyA Anthranilate phosphoribosyltransferase from sulfolobus solfataricus in complex with prpp and magnesium (see paper)
35% identity, 54% coverage: 199:485/531 of query aligns to 2:286/344 of 1zxyA

query
sites
1zxyA

T

N

L

I

Q

N

P

E

I

I

L

L

E

K

K

K

L

L

Y

I

Q

N

A

K

Q

S

T

D

L

L

S

E

Q

I

Q

N

E

E

S

A

H

E

Q

E

L

L

F

A

S

K

A

A

V

I

R

R

G

G

E

E

L

V

K

P

P

E

E

I

Q

L

L

V

A

S

A

A

A

I

L

L

V

V

S

A

M

L

K

R

I

M

R

K

G

G

E

E

H

S

P

K

N

N

E

E

I

I

A

V

G

G

A

F

A

A

T

R

A

A

L

M

L

R

E

E

N

L

A

A

A

I

P

K

F

I

P

D

R

V

P

P

D

N

Y

A

L

I

F

-

A

-

D

D

I

T

V
x
A

G
|
G

T

T

G

G

D
|
D

G

G

S

L

N

G

S
x
T

I

V

N
|
N

I

V

S
|
S

T
|
T

A

A

S

S

A

A

F

I

V

L

A

L

A

S

A

L

C

V

G

N

L

-

K

P

V

V

A

A

K
|
K

H

H

G

G

N

N

R

R

S

A

V

V

S

S

S

G

K

K

S

S

G

G

S
|
S

S

A

D

D

L

V

L

L

A

E

A

A

F

L

G

G

I

Y

N

N

L

I

D

I

M

V

N

P

A

P

D

E

K

R

S

A

R

K

Q

E

A

L

L

V

D

N

E

K

L

T

G

N

V

F

C

V

F

F

L

L

F

F

A

A

P

Q

K

Y

Y

Y

H

H

T

P

G

A

F

M

R

K

H

N

A

V

M

A

P

N

V

V

R

R

Q

K

Q

T

L

L

K

G

T

I

R

R

T

T

L

I

F

F

N

N

V

I

L

L

G

G

P

P

L

L

I

T

N

N

P

P

A

A

H

N

P

A

P

K

L

Y

A

Q

L

L

I

M

G

G

V

V

Y

F

S

S

P

K

E

D

L

H

V

L

L

D

P

L

I

L

A

S

E

K

T

S

L

A

R

Y

V

E

L

L

G

D

Y

F

Q

N

R

K

A

I

V

L

V

V

H

Y

S

G

G

E

-

P

G

G

M

I

D
|
D

E
|
E

V

V

S

S

L

P

H

I

A

G

P

N

T

T

I

F

V

M

A

K

E

I

L

V

H

S

D

K

G

R

E

G

I

I

K

E

S

E

Y

V

Q

K

L

L

T

N

A

V

E

T

D

D

F

F

G

G

L

I

T

S

P

P

Y

I

H

P

Q

I

E

E

Q

K

L

L

A

I

G

V

G

N

T

S

P

A

E

E

E

D

N

S

R

A

D

I

I

K

L

I

T

V

R

R

L

A

L

F

Q

L

G

G

K

K

G

D

D

E

A

H

A

V

H

A

E

E

binding magnesium ion: D223 (= D422), E224 (= E423)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: A78 (≠ V277), G79 (= G278), D83 (= D282), T87 (≠ S286), N89 (= N288), S91 (= S290), T92 (= T291), K106 (= K306), S118 (= S318), D223 (= D422), E224 (= E423)

2gvqD Anthranilate phosphoribosyl-transferase (trpd) from s. Solfataricus in complex with anthranilate (see paper)
35% identity, 54% coverage: 199:485/531 of query aligns to 2:286/345 of 2gvqD

query
sites
2gvqD

T

N

L

I

Q

N

P

E

I

I

L

L

E

K

K

K

L

L

Y

I

Q

N

A

K

Q

S

T

D

L

L

S

E

Q

I

Q

N

E

E

S

A

H

E

Q

E

L

L

F

A

S

K

A

A

V

I

R

R

G

G

E

E

L

V

K

P

P

E

E

I

Q

L

L

V

A

S

A

A

A

I

L

L

V

V

S

A

M

L

K

R

I

M

R

K

G

G

E

E

H

S

P

K

N

N

E

E

I

I

A

V

G

G

A

F

A

A

T

R

A

A

L

M

L

R

E

E

N

L

A

A

A

I

P

K

F

I

P

D

R

V

P

P

D

N

Y

A

L

I

F

-

A

-

D

D

I

T

V

A

G

G

T

T

G

G

D

D

G

G

S

L

N

G

S

T

I

V

N

N

I

V

S

S

T

T

A

A

S

S

A

A

F

I

V

L

A

L

A

S

A

L

C

V

G

N

L

-

K

P

V

V

A

A

K

K

H
|
H

G
|
G

N
|
N

R

R

S

A

V

V

S

S

S

G

K

K

S

S

G

G

S

S

S

A

D

D

L

V

L

L

A

E

A

A

F

L

G

G

I

Y

N

N

L

I

D

I

M

V

N

P

A

P

D

E

K

R

S

A

R

K

Q

E

A

L

L

V

D

N

E

K

L

T

G

N

V

F

C

V

F

F

L

L

F

F

A

A

P
x
Q

K

Y

Y

Y

H
|
H

T

P

G

A

F
x
M

R

K

H

N

A

V

M

A

P

N

V

V

R
|
R

Q

K

Q

T

L

L

K

G

T

I

R

R

T

T

L

I

F

F

N

N

V

I

L

L

G
|
G

P
|
P

L

L

I

T

N

N

P

P

A

A

H

N

P

A

P

K

L

Y

A

Q

L

L

I

M

G

G

V

V

Y

F

S

S

P

K

E

D

L

H

V

L

L

D

P

L

I

L

A

S

E

K

T

S

L

A

R

Y

V

E

L

L

G

D

Y

F

Q

N

R

K

A

I

V

L

V

V

H

Y

S

G

G

E

-

P

G

G

M

I

D

D

E

E

V

V

S

S

L

P

H

I

A

G

P

N

T

T

I

F

V

M

A

K

E

I

L

V

H

S

D

K

G

R

E

G

I

I

K

E

S

E

Y

V

Q

K

L

L

T

N

A

V

E

T

D

D

F

F

G

G

L

I

T

S

P

P

Y

I

H

P

Q

I

E

E

Q

K

L

L

A

I

G

V

G

N

T

S

P

A

E

E

E

D

N

S

R

A

D

I

I

K

L

I

T

V

R

R

L

A

L

F

Q

L

G

G

K

K

G

D

D

E

A

H

A

V

H

A

E

E

binding 2-aminobenzoic acid: H107 (= H307), G108 (= G308), N109 (= N309), N109 (= N309), Q151 (≠ P351), H154 (= H354), M157 (≠ F357), R164 (= R364), G177 (= G377), P178 (= P378)

P50384 Anthranilate phosphoribosyltransferase; EC 2.4.2.18 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see 2 papers)
35% identity, 54% coverage: 199:485/531 of query aligns to 2:286/345 of P50384

query
sites
P50384

T

N

L

I

Q

N

P

E

I

I

L

L

E

K

K

K

L

L

Y

I

Q

N

A

K

Q

S

T

D

L

L

S

E

Q

I

Q

N

E

E

S

A

H

E

Q

E

L

L

F

A

S

K

A

A

V

I

R

R

G

G

E

E

L

V

K

P

P

E

E

I

Q

L

L

V

A

S

A

A

A

I

L

L

V

V

S

A

M

L

K

R

I

M

R

K

G

G

E

E

H

S

P

K

N

N

E

E

I

I

A

V

G

G

A

F

A

A

T

R

A

A

L

M

L

R

E

E

N

L

A

A

A

I

P

K

F

I

P

D

R

V

P

P

D

N

Y

A

L

I

F

-

A

-

D

D

I

T

V

A

G

G

T

T

G

G

D

D

G

G

S

L

N

G

S
x
T

I

V

N

N

I

V

S

S

T

T

A

A

S

S

A

A

F

I

V

L

A

L

A

S

A

L

C

V

G

N

L

-

K

P

V

V

A

A

K
|
K

H
|
H

G

G

N

N

R

R

S

A

V

V

S

S

S

G

K

K

S

S

G

G

S
|
S

S

A

D

D

L

V

L

L

A

E

A

A

F

L

G

G

I

Y

N

N

L

I

D

I

M

V

N

P

A

P

D

E

K

R

S

A

R

K

Q

E

A

L

L

V

D

N

E

K

L

T

G

N

V

F

C

V

F

F

L

L

F

F

A

A

P

Q

K

Y

Y

Y

H
|
H

T

P

G

A

F

M

R

K

H

N

A

V

M

A

P

N

V

V

R
|
R

Q

K

Q

T

L

L

K

G

T

I

R

R

T

T

L

I

F

F

N

N

V

I

L

L

G

G

P

P

L

L

I

T

N

N

P

P

A

A

H

N

P

A

P

K

L

Y

A

Q

L

L

I

M

G

G

V

V

Y

F

S

S

P

K

E

D

L

H

V

L

L

D

P

L

I

L

A

S

E

K

T

S

L

A

R

Y

V

E

L

L

G

D

Y

F

Q

N

R

K

A

I

V

L

V

V

H

Y

S

G

G

E

-

P

G

G

M

I

D
|
D

E
|
E

V

V

S

S

L

P

H

I

A

G

P

N

T

T

I

F

V

M

A

K

E

I

L

V

H

S

D

K

G

R

E

G

I

I

K

E

S

E

Y

V

Q

K

L

L

T

N

A

V

E

T

D

D

F

F

G

G

L

I

T

S

P

P

Y

I

H

P

Q

I

E

E

Q

K

L

L

A

I

G

V

G

N

T

S

P

A

E

E

E

D

N

S

R

A

D

I

I

K

L

I

T

V

R

R

L

A

L

F

Q

L

G

G

K

K

G

D

D

E

A

H

A

V

H

A

E

E

T87 (≠ S286) binding
K106 (= K306) mutation to Q: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency dedreases only 10-fold.
H107 (= H307) mutation to A: Limited effect on either affinity for anthranilate and catalytic efficiency. 300-fold decrease of the affinity for anthranilate, whereas catalytic efficiency remains nearly unchanged; when associated with A-178.
S118 (= S318) binding
H154 (= H354) mutation to A: Limited effect on either affinity for anthranilate and catalytic efficiency.
R164 (= R364) mutation to A: Strong decrease of the affinity for anthranilate, although only a moderate 7-fold decrease in catalytic efficiency.
D223 (= D422) mutation to N: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency is unchanged.
E224 (= E423) mutation to Q: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency is unchanged.

1gxbA Anthranilate phosphoribosyltransferase in complex with pyrophosphate and magnesium (see paper)
34% identity, 54% coverage: 199:485/531 of query aligns to 2:282/339 of 1gxbA

query
sites
1gxbA

T

N

L

I

Q

N

P

E

I

I

L

L

E

K

K

K

L

L

Y

I

Q

N

A

K

Q

S

T

D

L

L

S

E

Q

I

Q

N

E

E

S

A

H

E

Q

E

L

L

F

A

S

K

A

A

V

I

R

R

G

G

E

E

L

V

K

P

P

E

E

I

Q

L

L

V

A

S

A

A

A

I

L

L

V

V

S

A

M

L

K

R

I

M

R

K

G

G

E

E

H

S

P

K

N

N

E

E

I

I

A

V

G

G

A

F

A

A

T

R

A

A

L

M

L

R

E

E

N

L

A

A

A

I

P

K

F

I

P

D

R

V

P

P

D

N

Y

A

L

-

F

-

A

-

D

-

I

-

V

I

G

D

T

T

G

A

G

G

D

-

G

G

S

L

N

G

S

T

I

V

N
|
N

I

V

S
|
S

T
|
T

A

A

S

S

A

A

F

I

V

L

A

L

A

S

A

L

C

V

G

N

L

-

K

P

V

V

A

A

K
|
K

H

H

G

G

N

N

R

R

S

A

V

V

S

S

S

G

K

K

S

S

G

G

S
|
S

S

A

D

D

L

V

L

L

A

E

A

A

F

L

G

G

I

Y

N

N

L

I

D

I

M

V

N

P

A

P

D

E

K

R

S

A

R

K

Q

E

A

L

L

V

D

N

E

K

L

T

G

N

V

F

C

V

F

F

L

L

F

F

A

A

P

Q

K

Y

Y

Y

H

H

T

P

G

A

F

M

R

K

H

N

A

V

M

A

P

N

V

V

R

R

Q

K

Q

T

L

L

K

G

T

I

R

R

T

T

L

I

F

F

N

N

V

I

L

L

G

G

P

P

L

L

I

T

N

N

P

P

A

A

H

N

P

A

P

K

L

Y

A

Q

L

L

I

M

G

G

V

V

Y

F

S

S

P

K

E

D

L

H

V

L

L

D

P

L

I

L

A

S

E

K

T

S

L

A

R

Y

V

E

L

L

G

D

Y

F

Q

N

R

K

A

I

V

L

V

V

H

Y

S

G

G

E

-

P

G

G

M

I

D

D

E
|
E

V

V

S

S

L

P

H

I

A

G

P

N

T

T

I

F

V

M

A

K

E

I

L

V

H

S

D

K

G

R

E

G

I

I

K

E

S

E

Y

V

Q

K

L

L

T

N

A

V

E

T

D

D

F

F

G

G

L

I

T

S

P

P

Y

I

H

P

Q

I

E

E

Q

K

L

L

A

I

G

V

G

N

T

S

P

A

E

E

E

D

N

S

R

A

D

I

I

K

L

I

T

V

R

R

L

A

L

F

Q

L

G

G

K

K

G

D

D

E

A

H

A

V

H

A

E

E

binding magnesium ion: S87 (= S290), E220 (= E423)
binding pyrophosphate 2-: N85 (= N288), S87 (= S290), T88 (= T291), K102 (= K306), S114 (= S318)

4owmA Anthranilate phosphoribosyl transferase from mycobacterium tuberculosis in complex with 3-fluoroanthranilate, prpp and magnesium (see paper)
36% identity, 49% coverage: 203:463/531 of query aligns to 6:268/346 of 4owmA

query
sites
4owmA

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

Q

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

M

S

D

A

Q

V

I

V

M

R

T

G

G

E

N

L

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K

T

I

M

R

K

G

A

E

P

H

T

P

A

N

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L

M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V
|
V

G
|
G

T

T

G

G

G
|
G

D

D

G

G

S

V

N

N

S

T

I

V

N
|
N

I

L

S
|
S

T
|
T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K
|
K

H

H

G

G

N

N

R

R

S

A

V
x
A

S
|
S

K

L

S

S

G
|
G

S

G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

V

R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

C

F

F

A

A

P
|
P

K

R

Y

F

H
|
H

T

P

G

S

F
x
Y

R
|
R

H

H

A

A

M
x
A

P

A

V

V

R

R

Q
x
R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G

G

P

P

L

L

I

T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E

D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D
|
D

E
|
E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

I

T

V

I

A

W

E

R

L

V

H

A

D

A

G

G

E

S

I

V

K

D

S

K

Y

L

Q

T

L

F

T

D

A

P

E

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

active site: V82 (= V277)
binding 2-azanyl-3-fluoranyl-benzoic acid: P156 (= P351), H159 (= H354), Y162 (≠ F357), R163 (= R358), A166 (≠ M361), R170 (≠ Q365)
binding magnesium ion: S95 (= S290), D227 (= D422), E228 (= E423), E228 (= E423)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G278), G86 (= G281), N93 (= N288), S95 (= S290), T96 (= T291), K111 (= K306), A117 (≠ V312), S118 (= S313), S119 (= S314), G122 (= G317)

4gkmA Bianthranilate-like analogue bound in the outer site of anthranilate phosphoribosyltransferase (anprt; trpd) (see paper)
36% identity, 49% coverage: 203:463/531 of query aligns to 6:268/346 of 4gkmA

query
sites
4gkmA

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

Q

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

M

S

D

A

Q

V

I

V

M

R

T

G

G

E

N

L

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K

T

I

M

R

K

G

A

E

P

H

T

P

A

N

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L

M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V
|
V

G

G

T

T

G

G

G
|
G

D

D

G

G

S

V

N

N

S

T

I

V

N
|
N

I

L

S
|
S

T
|
T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K
|
K

H

H

G

G

N
|
N

R

R

S

A

V
x
A

S
|
S

K

L

S

S

G
|
G

S

G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

V

R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

C

F

F

A
|
A

P
|
P

K

R

Y

F

H

H

T

P

G

S

F
x
Y

R
|
R

H

H

A

A

M
x
A

P

A

V

V

R
|
R

Q
x
R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G

G

P

P

L

L

I

T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E

D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D
|
D

E
|
E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

I

T

V

I

A

W

E

R

L

V

H

A

D

A

G

G

E

S

I

V

K

D

S

K

Y

L

Q

T

L

F

T

D

A

P

E

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

active site: V82 (= V277)
binding 2-[(2-carboxyphenyl)amino]-5-methylbenzoic acid: N114 (= N309), A155 (= A350), P156 (= P351), Y162 (≠ F357), R163 (= R358), A166 (≠ M361), R169 (= R364), R170 (≠ Q365)
binding magnesium ion: S95 (= S290), D227 (= D422), E228 (= E423), E228 (= E423)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G86 (= G281), N93 (= N288), S95 (= S290), T96 (= T291), K111 (= K306), A117 (≠ V312), S118 (= S313), S119 (= S314), G122 (= G317)

3r6cA Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor acs179) (see paper)
36% identity, 49% coverage: 203:463/531 of query aligns to 6:268/346 of 3r6cA

query
sites
3r6cA

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

Q

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

M

S

D

A

Q

V

I

V

M

R

T

G

G

E

N

L

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K

T

I

M

R

K

G

A

E

P

H

T

P

A

N

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L
x
M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V
|
V

G
|
G

T

T

G
|
G

D

D

G

G

S

V

N

N

S

T

I

V

N
|
N

I

L

S
|
S

T
|
T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K
|
K

H

H

G

G

N
|
N

R
|
R

S
x
A

V
x
A

S
|
S

K

L

S

S

G
|
G

S
x
G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

V

R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

C

F

F

A
|
A

P
|
P

K

R

Y

F

H
|
H

T

P

G

S

F
x
Y

R

R

H

H

A

A

M
x
A

P

A

V

V

R
|
R

Q

R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G
|
G

P

P

L

L

I
x
T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E

D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D
|
D

E
|
E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

I

T

V

I

A

W

E
x
R

L

V

H
x
A

D

A

G

G

E

S

I

V

K
x
D

S

K

Y

L

Q

T

L

F

T

D

A

P

E

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

active site: V82 (= V277)
binding 8-methoxyphenanthro[3,4-d][1,3]dioxole-5,6-dicarboxylic acid: M62 (≠ L259), N114 (= N309), A155 (= A350), P156 (= P351), H159 (= H354), Y162 (≠ F357), A166 (≠ M361), R169 (= R364), G182 (= G377), T185 (≠ I380), R239 (≠ E434), A241 (≠ H436), D246 (≠ K441)
binding magnesium ion: S95 (= S290), D227 (= D422), E228 (= E423), E228 (= E423)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G278), G85 (= G280), G86 (= G281), N93 (= N288), S95 (= S290), T96 (= T291), K111 (= K306), N114 (= N309), R115 (= R310), A116 (≠ S311), A117 (≠ V312), S118 (= S313), S119 (= S314), G122 (= G317), G123 (≠ S318)

Sites not aligning to the query:

binding 8-methoxyphenanthro[3,4-d][1,3]dioxole-5,6-dicarboxylic acid: 301, 310, 312

3qsaA Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor tamu-a7)
36% identity, 49% coverage: 203:463/531 of query aligns to 6:268/346 of 3qsaA

query
sites
3qsaA

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

Q

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

M

S

D

A

Q

V

I

V

M

R

T

G

G

E

N

L

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K

T

I

M

R

K

G

A

E

P

H

T

P

A

N

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L
x
M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V
|
V

G
|
G

T

T

G

G

G
|
G

D

D

G

G

S

V

N

N

S

T

I

V

N
|
N

I

L

S
|
S

T
|
T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K
|
K

H

H

G

G

N
|
N

R

R

S

A

V
x
A

S
|
S

K

L

S

S

G
|
G

S

G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

V

R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

C

F

F

A
|
A

P
|
P

K

R

Y

F

H
|
H

T

P

G

S

F
x
Y

R
|
R

H

H

A

A

M
x
A

P

A

V

V

R

R

Q

R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G
|
G

P

P

L

L

I

T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E

D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D
|
D

E
|
E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

I

T

V

I

A

W

E

R

L

V

H

A

D

A

G

G

E

S

I

V

K

D

S

K

Y

L

Q

T

L

F

T

D

A

P

E

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

active site: V82 (= V277)
binding magnesium ion: S95 (= S290), D227 (= D422), E228 (= E423), E228 (= E423)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G278), G86 (= G281), N93 (= N288), S95 (= S290), T96 (= T291), K111 (= K306), A117 (≠ V312), S118 (= S313), S119 (= S314), G122 (= G317)
binding 4,4,4-trifluoro-1-(4-methoxyphenyl)butane-1,3-dione: M62 (≠ L259), N114 (= N309), A155 (= A350), P156 (= P351), H159 (= H354), Y162 (≠ F357), R163 (= R358), A166 (≠ M361), G182 (= G377)

3qs8A Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor acs174) (see paper)
36% identity, 49% coverage: 203:463/531 of query aligns to 6:268/346 of 3qs8A

query
sites
3qs8A

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

Q

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

M

S

D

A

Q

V

I

V

M

R

T

G

G

E

N

L

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K

T

I

M

R

K

G

A

E

P

H

T

P

A

N

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L
x
M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V
|
V

G
|
G

T

T

G
|
G

D

D

G

G

S

V

N

N

S

T

I

V

N
|
N

I

L

S
|
S

T
|
T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K
|
K

H

H

G

G

N
|
N

R

R

S
x
A

V
x
A

S
|
S

K

L

S

S

G
|
G

S

G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

V

R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

C

F

F

A
|
A

P
|
P

K

R

Y

F

H

H

T

P

G

S

F
x
Y

R

R

H

H

A

A

M
x
A

P

A

V

V

R
|
R

Q
x
R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L
|
L

G
|
G

P

P

L

L

I

T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E

D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D
|
D

E
|
E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

I

T

V

I

A

W

E

R

L

V

H

A

D

A

G

G

E

S

I

V

K

D

S

K

Y

L

Q

T

L

F

T

D

A

P

E

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

active site: V82 (= V277)
binding 2-benzylbenzoic acid: M62 (≠ L259), G83 (= G278), N114 (= N309), N114 (= N309), A155 (= A350), A155 (= A350), P156 (= P351), Y162 (≠ F357), Y162 (≠ F357), A166 (≠ M361), R169 (= R364), R169 (= R364), R170 (≠ Q365), L181 (= L376), G182 (= G377)
binding magnesium ion: S95 (= S290), D227 (= D422), E228 (= E423), E228 (= E423)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G278), G85 (= G280), G86 (= G281), N93 (= N288), S95 (= S290), T96 (= T291), K111 (= K306), N114 (= N309), A116 (≠ S311), A117 (≠ V312), S118 (= S313), S119 (= S314), G122 (= G317), E228 (= E423)

3qqsA Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor acs172) (see paper)
36% identity, 49% coverage: 203:463/531 of query aligns to 6:268/346 of 3qqsA

query
sites
3qqsA

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

Q

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

M

S

D

A

Q

V

I

V

M

R

T

G

G

E

N

L

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K

T

I

M

R

K

G

A

E

P

H

T

P

A

N

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L

M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V
|
V

G

G

T

T

G
|
G

D

D

G

G

S

V

N

N

S

T

I

V

N
|
N

I

L

S
|
S

T
|
T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K
|
K

H

H

G

G

N

N

R
|
R

S

A

V
x
A

S
|
S

K

L

S

S

G
|
G

S

G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

V

R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

C

F

F

A

A

P
|
P

K

R

Y

F

H
|
H

T

P

G

S

F
x
Y

R
|
R

H

H

A

A

M
x
A

P

A

V

V

R
|
R

Q
x
R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G

G

P

P

L

L

I

T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E

D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D
|
D

E
|
E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

I

T

V

I

A

W

E

R

L

V

H

A

D

A

G

G

E

S

I

V

K

D

S

K

Y

L

Q

T

L

F

T

D

A

P

E

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

active site: V82 (= V277)
binding 2,2'-iminodibenzoic acid: P156 (= P351), H159 (= H354), Y162 (≠ F357), R163 (= R358), A166 (≠ M361), R169 (= R364), R170 (≠ Q365)
binding magnesium ion: S95 (= S290), D227 (= D422), E228 (= E423), E228 (= E423)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V82 (= V277), G85 (= G280), G86 (= G281), N93 (= N288), S95 (= S290), T96 (= T291), K111 (= K306), R115 (= R310), A117 (≠ V312), S118 (= S313), S119 (= S314), G122 (= G317)

1zvwA The crystal structure of trpd (rv2192c) from mycobacterium tuberculosis in complex with prpp and magnesium (see paper)
36% identity, 49% coverage: 203:463/531 of query aligns to 6:268/346 of 1zvwA

query
sites
1zvwA

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

Q

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

M

S

D

A

Q

V

I

V

M

R

T

G

G

E

N

L

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K
x
T

I
x
M

R
x
K

G

A

E
x
P

H

T

P

A

N

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L

M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V
|
V

G
|
G

T

T

G

G

D

D

G

G

S

V

N

N

S

T

I

V

N
|
N

I

L

S
|
S

T
|
T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K

K

H

H

G

G

N

N

R

R

S

A

V
x
A

S
|
S

K

L

S

S

G

G

S

G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

V

R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

C

F

F

A

A

P

P

K

R

Y

F

H

H

T

P

G

S

F

Y

R

R

H

H

A

A

M

A

P

A

V

V

R

R

Q

R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G

G

P

P

L

L

I

T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E
x
D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D
|
D

E
|
E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

I

T

V

I

A

W

E

R

L

V

H

A

D

A

G

G

E

S

I

V

K

D

S

K

Y

L

Q

T

L

F

T

D

A

P

E

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

active site: V82 (= V277)
binding benzamidine: T46 (≠ K243), M47 (≠ I244), K48 (≠ R245), P50 (≠ E247), D201 (≠ E396)
binding magnesium ion: S95 (= S290), D227 (= D422), E228 (= E423), E228 (= E423)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G278), N93 (= N288), S95 (= S290), T96 (= T291), A117 (≠ V312), S118 (= S313), S119 (= S314)

P9WFX5 Anthranilate phosphoribosyltransferase; EC 2.4.2.18 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
36% identity, 49% coverage: 203:463/531 of query aligns to 30:292/370 of P9WFX5

query
sites
P9WFX5

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

Q

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

M

S

D

A

Q

V

I

V

M

R

T

G

G

E

N

L

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K

T

I

M

R

K

G

A

E

P

H

T

P

A

N

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L

M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V

V

G
|
G

T

T

G

G

D

D

G

G

S

V

N

N

S
x
T

I

V

N

N

I

L

S

S

T

T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K

K

H

H

G

G

N

N

R

R

S

A

V

A

S

S

K

L

S

S

G

G

S
x
G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

V

R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

C

F

F

A

A

P

P

K

R

Y

F

H

H

T

P

G

S

F

Y

R

R

H

H

A

A

M

A

P

A

V

V

R

R

Q

R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G

G

P

P

L

L

I

T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E

D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D

D

E

E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

I

T

V

I

A

W

E

R

L

V

H

A

D

A

G

G

E

S

I

V

K

D

S

K

Y

L

Q

T

L

F

T

D

A

P

E

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

G107 (= G278) binding
T115 (≠ S286) binding
G147 (≠ S318) binding

3r88B Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor acs145) (see paper)
36% identity, 49% coverage: 203:463/531 of query aligns to 6:268/344 of 3r88B

query
sites
3r88B

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

Q

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

M

S

D

A

Q

V

I

V

M

R

T

G

G

E

N

L

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K

T

I

M

R

K

G

A

E

P

H

T

P

A

N

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L

M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V
|
V

G
|
G

T

T

G
|
G

D

D

G

G

S

V

N

N

S

T

I

V

N
|
N

I

L

S
|
S

T
|
T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K
|
K

H

H

G

G

N
|
N

R

R

S

A

V
x
A

S
|
S

K

L

S

S

G

G

S

G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

V

R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

C

F

F

A
|
A

P
|
P

K

R

Y

F

H
|
H

T

P

G

S

F
x
Y

R
|
R

H

H

A

A

M
x
A

P

A

V

V

R

R

Q

R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G

G

P

P

L

L

I

T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E

D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D
|
D

E
|
E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

I

T

V

I

A

W

E

R

L

V

H

A

D

A

G

G

E

S

I

V

K

D

S

K

Y

L

Q

T

L

F

T

D

A

P

E

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

active site: V82 (= V277)
binding 2-amino-4,5-dimethoxybenzoic acid: N114 (= N309), A155 (= A350), P156 (= P351), H159 (= H354), Y162 (≠ F357), R163 (= R358), A166 (≠ M361)
binding magnesium ion: S95 (= S290), D227 (= D422), E228 (= E423), E228 (= E423)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V82 (= V277), G83 (= G278), G85 (= G280), G86 (= G281), N93 (= N288), S95 (= S290), T96 (= T291), K111 (= K306), N114 (= N309), A117 (≠ V312), S118 (= S313), S119 (= S314)

4giuA Bianthranilate-like analogue bound in inner site of anthranilate phosphoribosyltransferase (anprt; trpd). (see paper)
36% identity, 49% coverage: 203:463/531 of query aligns to 7:269/346 of 4giuA

query
sites
4giuA

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

Q

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

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S

D

A

Q

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I

V

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T

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G

E

N

L

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K

T

I

M

R

K

G

A

E

P

H

T

P

A

N

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L

M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V
|
V

G
|
G

T

T

G
|
G

D

D

G

G

S

V

N

N

S

T

I

V

N
|
N

I

L

S
|
S

T
|
T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K
|
K

H

H

G
|
G

N
|
N

R

R

S
x
A

V
x
A

S
|
S

K

L

S

S

G
|
G

S

G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

V

R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

C

F

F

A
|
A

P

P

K

R

Y

F

H
|
H

T

P

G

S

F
x
Y

R

R

H

H

A

A

M

A

P

A

V

V

R
|
R

Q

R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G
|
G

P

P

L

L

I

T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E

D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D
|
D

E
|
E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

I

T

V

I

A

W

E

R

L

V

H

A

D

A

G

G

E

S

I

V

K

D

S

K

Y

L

Q

T

L

F

T

D

A

P

E

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

active site: V83 (= V277)
binding 2-[(2-carboxy-5-methylphenyl)amino]-3-methylbenzoic acid: G114 (= G308), N115 (= N309), A156 (= A350), H160 (= H354), Y163 (≠ F357), R170 (= R364), G183 (= G377)
binding magnesium ion: S96 (= S290), D228 (= D422), E229 (= E423), E229 (= E423)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G84 (= G278), G86 (= G280), G87 (= G281), N94 (= N288), S96 (= S290), T97 (= T291), K112 (= K306), N115 (= N309), A117 (≠ S311), A118 (≠ V312), S119 (= S313), S120 (= S314), G123 (= G317)

5c1rA Stereoisomer of prpp bound in the active site of mycobacterium tuberculosis anthranilate phosphoribosyl (anprt; trpd)
36% identity, 49% coverage: 203:463/531 of query aligns to 6:268/343 of 5c1rA

query
sites
5c1rA

I

I

L

L

E

G

K

R

L

L

Y

T

Q

D

A

N

Q

R

T

D

L

L

S

A

Q

R

Q

G

E

Q

S

A

H

A

Q

W

L

A

F

M

S

D

A

Q

V

I

V

M

R

T

G

G

E

N

L

A

K

R

P

P

E

A

Q

Q

L

I

A

A

A

F

L

A

V

V

S

A

M

M

K

T

I

M

R

K

G

A

E

P

H

T

P

A

N

D

E

E

I

V

A

G

G

E

A

L

A

A

T

G

A

V

L

M

L

L

E

S

N

H

A

A

A

H

P

P

F

L

P

P

R

A

-

D

-

T

-

V

P

P

D

D

Y

D

L

A

F

V

A

-

D

D

I

V

V
|
V

G
|
G

T

T

G

G

G
|
G

D
|
D

G

G

S
x
V

N

N

S

T

I

V

N
|
N

I

L

S
|
S

T
|
T

A

M

S

A

A

A

F

I

V

V

A

V

A

A

C

A

G

G

L

V

K

P

V

V

A

V

K
|
K

H

H

G

G

N

N

R
|
R

S

A

V
x
A

S
|
S

K

L

S

S

G

G

S

G

S

A

D

D

L

T

L

L

A

E

A

A

F

L

G

G

I

V

N

R

L

I

D

D

M

L

N

G

A

P

D

D

K

L

S

V

R

A

Q

R

A

S

L

L

D

A

E

E

L

V

G

G

V

I

C

G

F

F

L

C

F

F

A

A

P

P

K

R

Y

F

H

H

T

P

G

S

F

Y

R

R

H

H

A

A

M

A

P

A

V

V

R

R

Q

R

Q

E

L

I

K

G

T

V

R

P

T

T

L

V

F

F

N

N

V

L

L

L

G

G

P

P

L

L

I

T

N

N

P

P

A

A

H

R

P

P

P

R

L

A

A

G

L

L

I

I

G

G

V

C

Y

A

S

F

P

A

E

D

L

L

V

A

L

E

P

V

I

M

A

A

E

G

T

-

L

-

R

-

V

V

L

F

G

A

Y

A

Q

R

R

R

A

S

-

V

-

L

V

V

H

H

-

G

S

D

G

D

G

G

M

L

D

D

E
|
E

V

L

S

T

L

T

H

T

A

T

P

T

T

S

I

T

V

I

A

W

E

R

L

V

H

A

D

A

G

G

E

S

I

V

K

D

S

K

Y

L

Q

T

L

F

T

D

A

P

E

A

D

G

F

F

G

G

L

F

T

A

P

R

Y

A

H

Q

Q

L

E

D

Q

Q

L

L

A

A

G

G

active site: V82 (= V277)
binding 5-O-[(R)-hydroxy(phosphonooxy)phosphoryl]-1-O-phosphono-alpha-D-ribofuranose: V82 (= V277), G83 (= G278), G86 (= G281), N93 (= N288), S95 (= S290), T96 (= T291), K111 (= K306), R115 (= R310), A117 (≠ V312), S118 (= S313), S119 (= S314)
binding magnesium ion: D87 (= D282), V89 (≠ S284), S95 (= S290), E228 (= E423)

Query Sequence

>15383 FitnessBrowser__Keio:15383
MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERLATMSNPVLMLSPGPGV
PSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAGEILHGKASSIEHDGQAMF
AGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHDADRVCGFQFHPESILTTQGA
RLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQESHQLFSAVVRGELKPEQLAAALV
SMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADIVGTGGDGSNSINISTASAFVAAAC
GLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNADKSRQALDELGVCFLFAPKYHTGFRHA
MPVRQQLKTRTLFNVLGPLINPAHPPLALIGVYSPELVLPIAETLRVLGYQRAAVVHSGG
MDEVSLHAPTIVAELHDGEIKSYQLTAEDFGLTPYHQEQLAGGTPEENRDILTRLLQGKG
DAAHEAAVAANVAMLMRLHGHEDLQANAQTVLEVLRSGSAYDRVTALAARG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory