SitesBLAST
Comparing 15437 FitnessBrowser__Keio:15437 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4g9bA Crystal structure of beta-phosphoglucomutase homolog from escherichia coli, target efi-501172, with bound mg, open lid
100% identity, 100% coverage: 1:219/219 of query aligns to 2:220/227 of 4g9bA
- active site: D10 (= D9), L11 (= L10), D12 (= D11), T18 (= T17), K46 (= K45), S117 (= S116), V118 (= V117), K148 (= K147), E172 (= E171), D173 (= D172)
- binding magnesium ion: D10 (= D9), D12 (= D11), D173 (= D172)
P71447 Beta-phosphoglucomutase; Beta-PGM; EC 5.4.2.6 from Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) (see 3 papers)
44% identity, 98% coverage: 3:216/219 of query aligns to 2:216/221 of P71447
- D8 (= D9) modified: 4-aspartylphosphate; mutation to A: Inactive.; mutation to E: Inactive.
- D10 (= D11) mutation to A: Inactive.; mutation to E: Inactive.; mutation to N: Inactive.; mutation to S: Inactive.
- T16 (= T17) mutation to P: 500-fold reduction in the rate constant for Asp-8 phosphorylation by beta-G1,6bisP. 6,700-fold reduction in the apparent rate constant for cycling of the phosphorylated enzyme to convert beta-G1P to G6P. 13-fold increase in the estimated rate constant for phosphoryl transfer from the phospho-Asp8 to water.
- H20 (= H21) mutation to A: Impairs Asp-8 phosphorylation by beta-G1,6bisP and phosphoryl transfer from the phospho-Asp8 to the substrate beta-G1P.; mutation to N: 300-fold reduction in the conversion of beta-G1P to G6P in the presence of beta-G1,6bisP.; mutation to Q: 8-fold reduction in the conversion of beta-G1P to G6P in the presence of beta-G1,6bisP.
- K45 (= K45) mutation to A: 20'000-fold decrease in kcat/KM.
- G46 (= G46) mutation to A: 1'000'000-fold decrease in kcat/KM.; mutation to P: 100'000-fold decrease in kcat/KM.; mutation to V: 10'000-fold decrease in kcat/KM.
- R49 (= R49) mutation to K: 1'000'000-fold decrease in kcat/KM.
- S52 (= S52) mutation to A: Wild-type activity.
- K76 (= K78) mutation to A: 100-fold reduction in the conversion of beta-G1P to G6P in the presence of beta-G1,6bisP.
- D170 (= D172) mutation to A: Impaired, but active with an increase in the affinity for G1P.
2wf9A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate, and beryllium trifluoride, crystal form 2 (see paper)
44% identity, 98% coverage: 3:216/219 of query aligns to 2:216/221 of 2wf9A
- active site: D8 (= D9), L9 (= L10), D10 (= D11), T16 (= T17), K45 (= K45), S114 (= S116), A115 (≠ V117), K145 (= K147), E169 (= E171), D170 (= D172)
- binding beryllium trifluoride ion: D8 (= D9), L9 (= L10), D10 (= D11), S114 (= S116), A115 (≠ V117), K145 (= K147)
- binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D11), H20 (= H21), G46 (= G46), V47 (≠ I47), R49 (= R49), S116 (= S118), K117 (≠ L119), N118 (= N120)
- binding 6-O-phosphono-alpha-D-glucopyranose: D10 (= D11), H20 (= H21), G46 (= G46), V47 (≠ I47), R49 (= R49), A115 (≠ V117), S116 (= S118), K117 (≠ L119), N118 (= N120)
- binding magnesium ion: D8 (= D9), D10 (= D11), D170 (= D172)
1o03A Structure of pentavalent phosphorous intermediate of an enzyme catalyzed phosphoryl transfer reaction observed on cocrystallization with glucose 6-phosphate (see paper)
44% identity, 98% coverage: 3:216/219 of query aligns to 2:216/221 of 1o03A
- active site: D8 (= D9), L9 (= L10), D10 (= D11), T16 (= T17), K45 (= K45), S114 (= S116), A115 (≠ V117), K145 (= K147), E169 (= E171), D170 (= D172)
- binding 1,6-di-O-phosphono-alpha-D-glucopyranose: D8 (= D9), L9 (= L10), D10 (= D11), H20 (= H21), G46 (= G46), V47 (≠ I47), R49 (= R49), S114 (= S116), A115 (≠ V117), S116 (= S118), K117 (≠ L119), K145 (= K147)
- binding magnesium ion: D8 (= D9), D10 (= D11), D170 (= D172)
1lvhA The structure of phosphorylated beta-phosphoglucomutase from lactoccocus lactis to 2.3 angstrom resolution (see paper)
44% identity, 98% coverage: 3:216/219 of query aligns to 2:216/221 of 1lvhA
- active site: D8 (= D9), L9 (= L10), D10 (= D11), T16 (= T17), K45 (= K45), S114 (= S116), A115 (≠ V117), K145 (= K147), E169 (= E171), D170 (= D172)
- binding magnesium ion: D8 (= D9), D10 (= D11), D170 (= D172)
5olwA 5-fluorotryptophan labeled beta-phosphoglucomutase in an open conformation (see paper)
44% identity, 98% coverage: 3:216/219 of query aligns to 2:216/224 of 5olwA
- active site: D8 (= D9), L9 (= L10), D10 (= D11), T16 (= T17), K45 (= K45), S114 (= S116), A115 (≠ V117), K145 (= K147), E169 (= E171), D170 (= D172)
- binding calcium ion: D8 (= D9), D10 (= D11), P89 (≠ V91), V92 (= V94), E124 (≠ A126), N127 (≠ E129), E169 (= E171), D170 (= D172), S171 (≠ A173)
6qzgA Beta-glucose 1,6-bisphosphonate bound to wild type beta- phosphoglucomutse in an open conformation.
44% identity, 98% coverage: 3:216/219 of query aligns to 2:216/219 of 6qzgA
- binding 3,7-anhydro-1,2,8-trideoxy-1,8-diphosphono-D-glycero-D-gulo-octitol: D8 (= D9), L9 (= L10), D10 (= D11), H20 (= H21), G46 (= G46), S114 (= S116), A115 (≠ V117), S116 (= S118), K117 (≠ L119), K145 (= K147)
- binding magnesium ion: D8 (= D9), D10 (= D11), D170 (= D172)
1z4nA Structure of beta-phosphoglucomutase with inhibitor bound alpha- galactose 1-phosphate cocrystallized with fluoride (see paper)
44% identity, 98% coverage: 3:216/219 of query aligns to 2:216/219 of 1z4nA
- active site: D8 (= D9), L9 (= L10), D10 (= D11), T16 (= T17), K45 (= K45), S114 (= S116), A115 (≠ V117), K145 (= K147), E169 (= E171), D170 (= D172)
- binding 1-O-phosphono-alpha-D-galactopyranose: H20 (= H21), W24 (= W25), V47 (≠ I47), R49 (= R49), S116 (= S118), K117 (≠ L119), N118 (= N120)
- binding magnesium ion: D8 (= D9), D10 (= D11), E169 (= E171), D170 (= D172)
6h91A Phosphorylated beta-phosphoglucomutase from lactococcus lactis in an open conformer to 2.4 a
44% identity, 98% coverage: 3:216/219 of query aligns to 2:216/218 of 6h91A
4c4rA Structure of beta-phosphoglucomutase in complex with a phosphonate analogue of beta-glucose-1-phosphate and magnesium trifluoride (see paper)
44% identity, 98% coverage: 3:216/219 of query aligns to 2:216/218 of 4c4rA
- active site: D8 (= D9), L9 (= L10), D10 (= D11), T16 (= T17), K45 (= K45), S114 (= S116), A115 (≠ V117), K145 (= K147), E169 (= E171), D170 (= D172)
- binding magnesium ion: D8 (= D9), D10 (= D11), D170 (= D172)
- binding trifluoromagnesate: D8 (= D9), L9 (= L10), D10 (= D11), S114 (= S116), A115 (≠ V117), K145 (= K147)
- binding (1R)-1,5-anhydro-1-(phosphonomethyl)-D-glucitol: D10 (= D11), H20 (= H21), W24 (= W25), L44 (= L44), G46 (= G46), V47 (≠ I47), R49 (= R49), S52 (= S52), S116 (= S118), K117 (≠ L119)
3zi4A The structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate and scandium tetrafluoride
44% identity, 98% coverage: 3:216/219 of query aligns to 2:216/218 of 3zi4A
- active site: D8 (= D9), L9 (= L10), D10 (= D11), T16 (= T17), K45 (= K45), S114 (= S116), A115 (≠ V117), K145 (= K147), E169 (= E171), D170 (= D172)
- binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D11), H20 (= H21), G46 (= G46), V47 (≠ I47), R49 (= R49), S116 (= S118), K117 (≠ L119)
- binding magnesium ion: D8 (= D9), D10 (= D11), D170 (= D172)
- binding Scandium Tetrafluoride: D8 (= D9), L9 (= L10), D10 (= D11), S114 (= S116), A115 (≠ V117), K145 (= K147)
2wf8A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate, glucose-1-phosphate and beryllium trifluoride (see paper)
44% identity, 98% coverage: 3:216/219 of query aligns to 2:216/218 of 2wf8A
- active site: D8 (= D9), L9 (= L10), D10 (= D11), T16 (= T17), K45 (= K45), S114 (= S116), A115 (≠ V117), K145 (= K147), E169 (= E171), D170 (= D172)
- binding beryllium trifluoride ion: D8 (= D9), L9 (= L10), D10 (= D11), S114 (= S116), A115 (≠ V117), K145 (= K147)
- binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D11), H20 (= H21), G46 (= G46), V47 (≠ I47), R49 (= R49), A115 (≠ V117), S116 (= S118), K117 (≠ L119)
- binding 1-O-phosphono-alpha-D-glucopyranose: D10 (= D11), H20 (= H21), W24 (= W25), L44 (= L44), G46 (= G46), V47 (≠ I47), R49 (= R49), S52 (= S52), A115 (≠ V117), S116 (= S118), K117 (≠ L119)
- binding magnesium ion: D8 (= D9), D10 (= D11), D170 (= D172)
2wf7A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphonate and aluminium tetrafluoride (see paper)
44% identity, 98% coverage: 3:216/219 of query aligns to 2:216/218 of 2wf7A
- active site: D8 (= D9), L9 (= L10), D10 (= D11), T16 (= T17), K45 (= K45), S114 (= S116), A115 (≠ V117), K145 (= K147), E169 (= E171), D170 (= D172)
- binding tetrafluoroaluminate ion: D8 (= D9), L9 (= L10), D10 (= D11), S114 (= S116), K145 (= K147)
- binding 6,7-dideoxy-7-phosphono-beta-D-gluco-heptopyranose: D10 (= D11), G46 (= G46), V47 (≠ I47), R49 (= R49), S116 (= S118), K117 (≠ L119), N118 (= N120)
- binding magnesium ion: D8 (= D9), D10 (= D11), D170 (= D172)
2wf6A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate and aluminium tetrafluoride (see paper)
44% identity, 98% coverage: 3:216/219 of query aligns to 2:216/218 of 2wf6A
- active site: D8 (= D9), L9 (= L10), D10 (= D11), T16 (= T17), K45 (= K45), S114 (= S116), A115 (≠ V117), K145 (= K147), E169 (= E171), D170 (= D172)
- binding tetrafluoroaluminate ion: D8 (= D9), L9 (= L10), D10 (= D11), S114 (= S116), K145 (= K147)
- binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D11), G46 (= G46), V47 (≠ I47), R49 (= R49), S116 (= S118), K117 (≠ L119)
- binding magnesium ion: D8 (= D9), D10 (= D11), D170 (= D172)
2wf5A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate and trifluoromagnesate (see paper)
44% identity, 98% coverage: 3:216/219 of query aligns to 2:216/218 of 2wf5A
- active site: D8 (= D9), L9 (= L10), D10 (= D11), T16 (= T17), K45 (= K45), S114 (= S116), A115 (≠ V117), K145 (= K147), E169 (= E171), D170 (= D172)
- binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D11), H20 (= H21), G46 (= G46), V47 (≠ I47), R49 (= R49), A115 (≠ V117), S116 (= S118)
- binding magnesium ion: D8 (= D9), D10 (= D11), D170 (= D172)
- binding trifluoromagnesate: D8 (= D9), L9 (= L10), D10 (= D11), S114 (= S116), A115 (≠ V117), K145 (= K147)
5ok0A Structure of the d10n mutant of beta-phosphoglucomutase from lactococcus lactis trapped with native reaction intermediate beta- glucose 1,6-bisphosphate to 2.2a resolution.
44% identity, 98% coverage: 3:216/219 of query aligns to 2:216/218 of 5ok0A
- active site: D8 (= D9), L9 (= L10), N10 (≠ D11), T16 (= T17), K45 (= K45), S114 (= S116), A115 (≠ V117), K145 (= K147), E169 (= E171), D170 (= D172)
- binding 1,6-di-O-phosphono-beta-D-glucopyranose: D8 (= D9), L9 (= L10), N10 (≠ D11), H20 (= H21), L44 (= L44), V47 (≠ I47), R49 (= R49), S114 (= S116), A115 (≠ V117), S116 (= S118)
- binding magnesium ion: D8 (= D9), N10 (≠ D11), D170 (= D172)
- binding 1,3-propandiol: I33 (= I34), N34 (≠ S35), G35 (vs. gap), V36 (≠ I36)
5o6rA Structure of beta-phosphoglucomutase d10n mutant in complex with glucose-1-phosphate and aluminium tetrafluoride
44% identity, 98% coverage: 3:216/219 of query aligns to 2:216/218 of 5o6rA
- active site: D8 (= D9), L9 (= L10), N10 (≠ D11), T16 (= T17), K45 (= K45), S114 (= S116), A115 (≠ V117), K145 (= K147), E169 (= E171), D170 (= D172)
- binding tetrafluoroaluminate ion: D8 (= D9), L9 (= L10), N10 (≠ D11), G46 (= G46), S114 (= S116), K145 (= K147)
- binding magnesium ion: D8 (= D9), N10 (≠ D11), D170 (= D172)
- binding 1-O-phosphono-beta-D-glucopyranose: H20 (= H21), W24 (= W25), L44 (= L44), V47 (≠ I47), R49 (= R49), S52 (= S52), S116 (= S118), K117 (≠ L119), N118 (= N120)
5o6pA Structure of beta-phosphoglucomutase d10n mutant in complex with glucose-1,6-bisphosphate
44% identity, 98% coverage: 3:216/219 of query aligns to 1:208/209 of 5o6pA
- active site: D7 (= D9), L8 (= L10), N9 (≠ D11), T15 (= T17), K44 (= K45), S113 (= S116), A114 (≠ V117), K144 (= K147), E168 (= E171), D169 (= D172)
- binding 1,6-di-O-phosphono-beta-D-glucopyranose: D7 (= D9), L8 (= L10), N9 (≠ D11), H19 (= H21), L43 (= L44), K44 (= K45), G45 (= G46), V46 (≠ I47), R48 (= R49), S113 (= S116), A114 (≠ V117), S115 (= S118), K116 (≠ L119), K144 (= K147)
- binding magnesium ion: D7 (= D9), N9 (≠ D11), D169 (= D172)
4c4sA Structure of beta-phosphoglucomutase in complex with an alpha- fluorophosphonate analogue of beta-glucose-1-phosphate and magnesium trifluoride (see paper)
44% identity, 98% coverage: 3:216/219 of query aligns to 2:213/215 of 4c4sA
- active site: D8 (= D9), L9 (= L10), D10 (= D11), T16 (= T17), K45 (= K45), S111 (= S116), A112 (≠ V117), K142 (= K147), E166 (= E171), D167 (= D172)
- binding (1R)-1,5-anhydro-1-[(S)-fluoro(phosphono)methyl]-D-glucitol: D10 (= D11), H20 (= H21), W24 (= W25), L44 (= L44), G46 (= G46), V47 (≠ I47), R49 (= R49), S113 (= S118)
- binding magnesium ion: D8 (= D9), D10 (= D11), D167 (= D172)
- binding trifluoromagnesate: D8 (= D9), L9 (= L10), D10 (= D11), S111 (= S116), A112 (≠ V117), K142 (= K147)
1te2A Putative phosphatase ynic from escherichia coli k12
31% identity, 85% coverage: 7:192/219 of query aligns to 7:189/218 of 1te2A
- active site: D9 (= D9), D11 (= D11), S17 (≠ T17), D44 (≠ K45), S111 (= S116), A112 (≠ V117), K144 (= K147), E168 (= E171), D169 (= D172)
- binding calcium ion: D9 (= D9), D11 (= D11), D169 (= D172)
- binding 2-phosphoglycolic acid: D9 (= D9), M10 (≠ L10), D11 (= D11), G47 (≠ S48), S111 (= S116), A112 (≠ V117), K144 (= K147)
Query Sequence
>15437 FitnessBrowser__Keio:15437
MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESLKGISRDESLRRILQHG
GKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLLADLRAQQISVGLASVSLN
APTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGLGVPPQACIGIEDAQAGIDAI
NASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQNV
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory