SitesBLAST
Comparing 15520 FitnessBrowser__Keio:15520 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 12 hits to proteins with known functional sites (download)
2y4oA Crystal structure of paak2 in complex with phenylacetyl adenylate (see paper)
67% identity, 98% coverage: 7:433/437 of query aligns to 6:433/433 of 2y4oA
- binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: F135 (≠ Y136), F140 (= F141), A213 (= A215), E214 (= E216), P215 (= P217), I235 (= I237), G237 (= G239), L238 (= L240), S239 (= S241), P244 (= P246), D304 (= D306), R325 (= R327), I331 (= I333), N336 (= N338)
2y4oB Crystal structure of paak2 in complex with phenylacetyl adenylate (see paper)
68% identity, 97% coverage: 7:432/437 of query aligns to 6:432/432 of 2y4oB
- binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: F135 (≠ Y136), F140 (= F141), G212 (= G214), A213 (= A215), E214 (= E216), P215 (= P217), I235 (= I237), G237 (= G239), L238 (= L240), S239 (= S241), P244 (= P246), D304 (= D306), R325 (= R327), I331 (= I333), N336 (= N338)
- binding magnesium ion: S204 (≠ C206), V228 (≠ L230)
2y27B Crystal structure of paak1 in complex with atp from burkholderia cenocepacia (see paper)
66% identity, 97% coverage: 7:432/437 of query aligns to 4:427/427 of 2y27B
- binding adenosine-5'-triphosphate: K65 (= K68), S90 (= S93), S91 (= S94), G92 (= G95), T93 (= T96), T94 (= T97), F138 (= F141), A211 (= A215), E212 (= E216), P213 (= P217), D232 (= D236), I233 (= I237), Y234 (= Y238), G235 (= G239), L236 (= L240), S237 (= S241), D302 (= D306), I320 (= I324), R323 (= R327), K419 (= K424)
- binding magnesium ion: V200 (≠ S204), S202 (≠ C206), L204 (= L208), M226 (≠ L230), G227 (= G231), Q347 (≠ F351), L350 (= L354)
2y4nA Paak1 in complex with phenylacetyl adenylate (see paper)
65% identity, 98% coverage: 7:433/437 of query aligns to 4:426/426 of 2y4nA
- binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: Y131 (= Y136), F136 (= F141), G138 (= G143), G208 (= G214), A209 (= A215), E210 (= E216), P211 (= P217), I231 (= I237), Y232 (= Y238), G233 (= G239), L234 (= L240), S235 (= S241), P240 (= P246), D300 (= D306), R321 (= R327), K417 (= K424)
- binding magnesium ion: V198 (≠ S204), S200 (≠ C206), Q345 (≠ F351), L348 (= L354)
4r1mA Crystal structure of a putative acyl-coa ligase (bt_0428) from bacteroides thetaiotaomicron vpi-5482 at 2.48 a resolution
45% identity, 97% coverage: 8:433/437 of query aligns to 9:434/435 of 4r1mA
- binding adenosine monophosphate: A215 (= A215), E216 (= E216), P217 (= P217), N236 (≠ D236), S237 (≠ I237), F238 (≠ Y238), G239 (= G239), M240 (≠ L240), T241 (≠ S241), D305 (= D306), R329 (= R327), I335 (= I333), N340 (= N338)
- binding zinc ion: C252 (= C252), H259 (≠ T260), C314 (vs. gap), C316 (vs. gap)
4r1lA Crystal structure of a putative acyl-coa ligase (bt_0428) from bacteroides thetaiotaomicron vpi-5482 at 2.42 a resolution
44% identity, 97% coverage: 8:433/437 of query aligns to 9:432/433 of 4r1lA
- binding adenosine-5'-diphosphate: A215 (= A215), E216 (= E216), P217 (= P217), S237 (≠ I237), F238 (≠ Y238), G239 (= G239), M240 (≠ L240), T241 (≠ S241), D305 (= D306), R329 (= R327), N340 (= N338)
- binding adenosine monophosphate: A215 (= A215), E216 (= E216), P217 (= P217), S237 (≠ I237), F238 (≠ Y238), G239 (= G239), M240 (≠ L240), T241 (≠ S241), D305 (= D306), R329 (= R327), N340 (= N338)
- binding coenzyme a: S136 (≠ A135), A164 (≠ G163), G165 (= G164), N166 (≠ Q165), S167 (≠ T166), I185 (≠ T184), Y188 (= Y187), K337 (≠ R335), T408 (≠ S407)
- binding zinc ion: C252 (= C252), H259 (≠ T260), C314 (vs. gap), C316 (vs. gap)
6he0A Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in complex with 2-hib-amp and coa in the thioesterfication state (see paper)
30% identity, 98% coverage: 11:437/437 of query aligns to 30:469/477 of 6he0A
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] 2-methyl-2-oxidanyl-propanoate: S241 (≠ G214), G242 (≠ A215), E243 (= E216), P244 (= P217), G267 (≠ Y238), S268 (≠ G239), M269 (≠ L240), A270 (≠ S241), D335 (= D306), I357 (= I324), N371 (= N338)
- binding adenosine monophosphate: G242 (≠ A215), E243 (= E216), P244 (= P217), C266 (≠ I237), G267 (≠ Y238), S268 (≠ G239), A270 (≠ S241), E271 (= E242), D335 (= D306), N371 (= N338)
- binding coenzyme a: Y166 (≠ F141), A188 (≠ G163), G189 (= G164), P191 (≠ T166), S194 (≠ Q169), Y210 (≠ M182), G211 (≠ V183), T212 (= T184), Y215 (= Y187), H218 (≠ N190), R368 (= R335), G369 (= G336), M401 (≠ L368), V439 (≠ I406), R440 (≠ S407)
6hdyA Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in the postadenylation state in complex with s3-hb-amp (see paper)
30% identity, 98% coverage: 11:437/437 of query aligns to 30:466/474 of 6hdyA
- binding (3s)-3-hydroxybutanoic acid: Y162 (≠ F141), S237 (≠ G214), G263 (≠ Y238), S264 (≠ G239), M265 (≠ L240), A266 (≠ S241), F271 (≠ G247)
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] (3~{S})-3-oxidanylbutanoate: Y162 (≠ F141), G164 (= G143), S237 (≠ G214), G238 (≠ A215), E239 (= E216), P240 (= P217), C262 (≠ I237), G263 (≠ Y238), S264 (≠ G239), A266 (≠ S241), F271 (≠ G247), D331 (= D306), I353 (= I324), R356 (= R327), K453 (= K424)
6hdxA Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in the postadenylation state in complex with r3-hib-amp (see paper)
30% identity, 98% coverage: 11:437/437 of query aligns to 30:466/474 of 6hdxA
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] (2~{R})-2-methyl-3-oxidanyl-propanoate: Y162 (≠ F141), G164 (= G143), S237 (≠ G214), G238 (≠ A215), E239 (= E216), P240 (= P217), C262 (≠ I237), G263 (≠ Y238), S264 (≠ G239), A266 (≠ S241), F271 (≠ G247), D331 (= D306), I353 (= I324), R356 (= R327), K453 (= K424)
- binding (2r)-3-hydroxy-2-methylpropanoic acid: Y162 (≠ F141), G164 (= G143), S237 (≠ G214), G263 (≠ Y238), S264 (≠ G239), A266 (≠ S241), F271 (≠ G247)
6siwA Paak family amp-ligase with amp (see paper)
30% identity, 67% coverage: 13:306/437 of query aligns to 8:295/432 of 6siwA
- binding adenosine monophosphate: S84 (= S94), A208 (= A215), E209 (= E216), P210 (= P217), E231 (≠ I237), Y232 (= Y238), G233 (= G239), S234 (≠ L240), T235 (≠ S241), D295 (= D306)
- binding magnesium ion: E75 (= E85), L77 (≠ V87), S83 (= S93), P116 (= P128), G143 (= G155), T145 (= T157), E236 (= E242)
- binding zinc ion: C244 (= C252), H250 (≠ T260)
Sites not aligning to the query:
6sixB Paak family amp-ligase with anp (see paper)
30% identity, 67% coverage: 13:306/437 of query aligns to 13:300/437 of 6sixB
- binding phosphoaminophosphonic acid-adenylate ester: S88 (= S93), S89 (= S94), A213 (= A215), E214 (= E216), P215 (= P217), E236 (≠ I237), Y237 (= Y238), G238 (= G239), S239 (≠ L240), T240 (≠ S241), E241 (= E242), D300 (= D306)
- binding magnesium ion: R79 (≠ M84), E80 (= E85), P121 (= P128), T150 (= T157)
- binding zinc ion: C249 (= C252), H255 (≠ T260)
Sites not aligning to the query:
6siyA Paak family amp-ligase with amp and substrate (see paper)
30% identity, 67% coverage: 13:306/437 of query aligns to 9:296/433 of 6siyA
- binding 3-hydroxyanthranilic acid: T125 (≠ Y136), P126 (≠ G137), T132 (≠ G143), L135 (≠ G146), R153 (vs. gap), N177 (vs. gap), A209 (= A215), E232 (≠ I237), G234 (= G239), S235 (≠ L240)
- binding adenosine monophosphate: S85 (= S94), A209 (= A215), E210 (= E216), P211 (= P217), E232 (≠ I237), Y233 (= Y238), G234 (= G239), S235 (≠ L240), T236 (≠ S241), D296 (= D306)
- binding magnesium ion: R75 (≠ M84), E76 (= E85), L78 (≠ V87), P117 (= P128), G144 (= G155), A145 (= A156), T146 (= T157)
Sites not aligning to the query:
Query Sequence
>15520 FitnessBrowser__Keio:15520
MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKFDAAGVHPDDFRELSDL
RKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTVVGYTQNDIDNWANIVARS
LRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIPMSGGQTEKQAQLIRDFQPDM
IMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPWTQAMRKEIERRLGITALDIYGL
SEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDGTPLADGEHGELLFTTLTKEALPVI
RYRTRDLTRLLPGTARTMRRMDRISGRSDDMLIIRGVNVFPSQLEEEIVKFEHLSPHYQL
EVNRRGHLDSLSVKVELKESSLTLTHEQRCQVCHQLRHRIKSMVGISTDVMIVNCGSIPR
SEGKACRVFDLRNIVGA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory