SitesBLAST

Q8ZPD3 L-methionine sulfoximine/L-methionine sulfone acetyltransferase; L-amino acid N-acyltransferase; Methionine derivative detoxifier A; MDDA; EC 2.3.1.-; EC 2.3.1.- from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
87% identity, 99% coverage: 1:170/172 of query aligns to 1:170/171 of Q8ZPD3

query
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Q8ZPD3

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E82 (= E82) mutation to Q: Loss of acetyltransferase activity.
VYV 85:87 (= VYV 85:87) binding
GKGLGR 93:98 (= GKGLGR 93:98) binding
N124 (= N124) binding
S133 (= S133) binding

2j8rA Structure of p. Aeruginosa acetyltransferase pa4866 solved in complex with l-methionine sulfoximine (see paper)
63% identity, 97% coverage: 2:168/172 of query aligns to 2:170/170 of 2j8rA

query
sites
2j8rA

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binding azide ion: W42 (= W42), Y66 (= Y65), S85 (= S84)
binding (2s)-2-amino-4-(methylsulfonimidoyl)butanoic acid: A27 (= A27), I28 (= I28), W29 (= W29), R73 (= R72), F75 (= F74), F78 (= F77), E83 (= E82)

A6VCX3 L-methionine sulfoximine/L-methionine sulfone acetyltransferase; Methionine derivative detoxifier A; MDDA; EC 2.3.1.- from Pseudomonas aeruginosa (strain PA7) (see paper)
63% identity, 97% coverage: 2:168/172 of query aligns to 4:172/172 of A6VCX3

query
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A6VCX3

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RPF 75:77 (≠ RSF 72:74) binding
EHS 85:87 (= EHS 82:84) binding

2j8mA Structure of p. Aeruginosa acetyltransferase pa4866 (see paper)
63% identity, 97% coverage: 2:168/172 of query aligns to 3:171/171 of 2j8mA

query
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2j8mA

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binding azide ion: W73 (= W71), R74 (= R72)

4jwpA Crystal structure of ribosomal-protein-alanine n-acetyltransferase from brucella melitensis in complex with acetyl coa
54% identity, 92% coverage: 1:159/172 of query aligns to 4:162/165 of 4jwpA

query
sites
4jwpA

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binding acetyl coenzyme *a: A30 (= A27), V88 (= V85), Y89 (= Y86), V90 (= V87), R95 (≠ Q92), G96 (= G93), G98 (= G95), G100 (= G97), R101 (= R98), G122 (= G119), A129 (= A126), S130 (= S127), A132 (≠ H129)
binding calcium ion: E110 (= E107), E113 (≠ D110)

4mbuA Crystal structure of n-acetyltransferase from staphylococcus aureus mu50 (see paper)
44% identity, 93% coverage: 3:162/172 of query aligns to 5:164/165 of 4mbuA

query
sites
4mbuA

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binding phosphate ion: Q134 (= Q132), K139 (≠ V137), H140 (≠ V138)

2jlmF Structure of a putative acetyltransferase (aciad1637) from acinetobacter baylyi adp1 (see paper)
40% identity, 90% coverage: 14:168/172 of query aligns to 22:177/180 of 2jlmF

query
sites
2jlmF

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binding azide ion: T34 (≠ A26), A35 (= A27), Y95 (= Y86), I96 (≠ V87), D130 (≠ E121), N133 (= N124)

4jxrB Crystal structure of a gnat superfamily phosphinothricin acetyltransferase (pat) from sinorhizobium meliloti in complex with accoa
37% identity, 96% coverage: 2:166/172 of query aligns to 4:169/185 of 4jxrB

query
sites
4jxrB

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R

H

E

A

S

V

V

L

L

Y

N

T

G

A

V

A
|
A

I

T

W

Y

N

E

D

E

Q

T

T

P

V

P

D

S

A

E

D

A

N

E

R

M

I

A

A

L

W

R

F

F

E

S

A

T

R

I

T

T

L

G

A

N

G

G

Y

Y

P

P

V

Y

L

V

V

V

S

A

-

L

E

D

E

E

N

R

G

G

V

A

V

V

T

I

G

G

Y

Y

A

A

S

Y

F

A

G

S

D

A

W

F

R

R

S

N

F

R

D

T

G

A

F

Y

R

R

H

F

T

L

V

V

E

E

H
x
D

S
|
S

V
x
I

Y
|
Y

V
x
L

H

S

P
|
P

D

E

H

A

Q
x
R

G
|
G

K

K

G

G

L

I

G
|
G

R

K

K

A

L

L

S

S

R

E

L

L

I

V

D

G

E

R

A

C

R

T

D

A

C

L

G

G

K

F

H

R

V

Q

M

M

V

I

A

A

G

V

I

I

E

G

S

G

Q

A

N
x
H

Q

P

A
x
S

S
|
S

L

I

H

A

L

L

H
|
H

Q

R

S

A

L

L

G

G

F

F

V

E

V

L

T

Q

A

G

Q

L

M

M

P

K

Q

A

V

T

G

G

T

F

K

K

F

H

G

G

R

R

W

W

L

L

D

D

L

T

T

A

F

F

M

M

Q

Q

L

R

Q

P

L

L

D

G

E

E

R

G

T

T

binding acetyl coenzyme *a: A29 (= A27), D86 (≠ H83), S87 (= S84), I88 (≠ V85), Y89 (= Y86), L90 (≠ V87), P92 (= P89), R95 (≠ Q92), G96 (= G93), G100 (= G97), H127 (≠ N124), S129 (≠ A126), S130 (= S127), H134 (= H131)

5dwnA Crystal structure of phosphinothricin n-acetyltransferase from brucella ovis in complex with acetylcoa
36% identity, 97% coverage: 3:168/172 of query aligns to 6:175/181 of 5dwnA

query
sites
5dwnA

I

I

R

R

F

D

A

F

R

Q

K

P

A

A

D

D

C

I

A

E

A

T

I

I

A

T

E

A

I

I

Y

Y

N

T

H

Q

A

A

V

V

L

L

Y

T

T

G

A

T

A

G

I

S

W

Y

N

E

D

I

Q

E

T

P

V

P

D

T

A

M

D

D

N

E

R

M

I

A

A

K

W

R

F

F

E

A

A

A

R

F

T

A

L

D

A

Q

G

G

Y

F

P

P

V

I

L

L

V

V

S

A

E

E

E

A

N

D

G

G

V

R

V

V

T

L

G

G

Y

Y

A

A

S

Y

F

A

G

S

D

Y

W

F

R

R

S

V

F

R

D

P

G

A

F

Y

R

R

H

W

T

L

V

A

E

E

H

D

S
|
S

V
x
I

Y
|
Y

V
x
I

H

A

P

P

D

D

H

A

Q
x
K

G
|
G

K

Q

G
|
G

L

I

G
|
G

R
x
K

K

L

L

L

S

R

R

E

L

L

I

I

D

A

E

R

A

I

R

S

D

A

C

L

G

G

K

F

H

R

V

Q

M

L

V

L

A

A

-

V

-

I

G

G

I

D

E

G

S

E

Q

H

N

N

Q

I

A
x
G

S
|
S

L

V

H
x
K

L
|
L

H
|
H

Q

E

S

S

L

L

G

G

F

F

V

T

V

H

T

C

A

G

Q

R

M

I

P

E

Q

G

V

S

G

G

T

F

K

K

F

H

G

G

R

R

W

W

L

L

D

D

L

T

T

V

F

L

M

M

Q

Q

L

L

Q

P

L

L

D

N

-

G

E

R

R

S

T

T

E

E

P

P

binding acetyl coenzyme *a: S87 (= S84), I88 (≠ V85), Y89 (= Y86), I90 (≠ V87), K95 (≠ Q92), G96 (= G93), G98 (= G95), G100 (= G97), K101 (≠ R98), G131 (≠ A126), S132 (= S127), K134 (≠ H129), L135 (= L130), H136 (= H131)

5t7eD Crystal structure of streptomyces hygroscopicus bialaphos resistance (bar) protein in complex with coenzyme a and l-phosphinothricin (see paper)
32% identity, 93% coverage: 3:162/172 of query aligns to 4:162/175 of 5t7eD

query
sites
5t7eD

I

I

R

R

F

R

A

A

R

T

K

E

A

A

D

D

C

M

A

P

A

A

I

V

A

C

E

T

I

I

Y

V

N

N

H

H

A
x
Y

V

I

L

E

Y

T

T

S

A

T

A
x
V

I

N

W
x
F

N

R

D

T

Q

E

T

P

V

Q

D

E

A

P

D

Q

N

E

R

-

I

-

A

-

W

W

F

T

E

D

-

D

-

L

A

V

R

R

T

L

L

R

A

E

G

R

Y

Y

P

P

V

W

L

L

V

V

S

A

E

E

E

V

N

D

G

G

V

E

V

V

T

A

G

G

Y

I

A

A

S

Y

F

A

G

G

D

P

W

W

R
x
K

S

A

F
x
R

D

N

G

A

F
x
Y

R

D

H

W

T

T

V

A

E

E

H

S

S
x
T

V
|
V

Y
|
Y

V
|
V

H

S

P

P

D

R

H

H

Q
|
Q

G
x
R

K
x
T

G
|
G

L

L

G
|
G

R
x
S

K

T

L

L

L

Y

S

T

R

H

L

L

I

L

D

K

E

S

A

L

R

E

D

A

C

Q

G

G

K

F

H

K

V

S

M

V

V

V

A

A

G
x
V

I

I

E
x
G

S

L

Q

P

N

N

Q

D

A
x
P

S
|
S

L

V

H

R

L
x
M

H

H

Q

E

S

A

L

L

G

G

F

Y

V

A

V

P

T

R

A

G

Q

M

M

L

P

R

Q

A

V

A

G

G

T

F

K

K

F

H

G

G

R

N

W

W

L

H

D

D

L

V

T

G

F

F

M

W

Q

Q

L

L

Q

D

L

F

binding coenzyme a: Y22 (≠ A21), V28 (≠ A27), V85 (= V85), Y86 (= Y86), V87 (= V87), Q92 (= Q92), R93 (≠ G93), T94 (≠ K94), G95 (= G95), G97 (= G97), S98 (≠ R98), P126 (≠ A126), S127 (= S127), M130 (≠ L130)
binding phosphinothricin: F30 (≠ W29), K72 (≠ R72), R74 (≠ F74), Y77 (≠ F77), T84 (≠ S84), Y86 (= Y86), V119 (≠ G119), G121 (≠ E121)

5t7dA Crystal structure of streptomyces hygroscopicus bialaphos resistance (bar) protein in complex with acetyl coenzyme a (see paper)
32% identity, 93% coverage: 3:162/172 of query aligns to 3:161/173 of 5t7dA

query
sites
5t7dA

I

I

R

R

F

R

A

A

R

T

K

E

A

A

D

D

C

M

A

P

A

A

I

V

A

C

E

T

I

I

Y

V

N

N

H

H

A
x
Y

V

I

L

E

Y

T

T

S

A

T

A
x
V

I

N

W

F

N

R

D

T

Q

E

T

P

V

Q

D

E

A

P

D

Q

N

E

R

-

I

-

A

-

W

W

F

T

E

D

-

D

-

L

A

V

R

R

T

L

L

R

A

E

G

R

Y

Y

P

P

V

W

L

L

V

V

S

A

E

E

E

V

N

D

G

G

V

E

V

V

T

A

G

G

Y

I

A

A

S

Y

F

A

G

G

D

P

W

W

R

K

S

A

F

R

D

N

G

A

F

Y

R

D

H

W

T

T

V

A

E

E

H

S

S
x
T

V
|
V

Y
|
Y

V
|
V

H

S

P

P

D

R

H

H

Q
|
Q

G
x
R

K

T

G
|
G

L

L

G
|
G

R
x
S

K

T

L

L

L

Y

S

T

R

H

L

L

I

L

D

K

E

S

A

L

R

E

D

A

C

Q

G

G

K

F

H

K

V

S

M

V

V

V

A

A

G
x
V

I

I

E

G

S

L

Q

P

N

N

Q

D

A
x
P

S
|
S

L

V

H
x
R

L
x
M

H

H

Q

E

S

A

L

L

G

G

F

Y

V

A

V

P

T

R

A

G

Q

M

M

L

P

R

Q

A

V

A

G

G

T

F

K

K

F

H

G

G

R

N

W

W

L

H

D

D

L

V

T

G

F

F

M

W

Q

Q

L

L

Q

D

L

F

binding acetyl coenzyme *a: Y21 (≠ A21), V27 (≠ A27), T83 (≠ S84), V84 (= V85), Y85 (= Y86), V86 (= V87), Q91 (= Q92), R92 (≠ G93), G94 (= G95), G96 (= G97), S97 (≠ R98), V118 (≠ G119), P125 (≠ A126), S126 (= S127), R128 (≠ H129), M129 (≠ L130)

6m7gA Crystal structure of arsn, n-acetyltransferase with substrate phosphinothricin from pseudomonas putida kt2440 (see paper)
33% identity, 99% coverage: 1:170/172 of query aligns to 2:171/176 of 6m7gA

query
sites
6m7gA

M

I

S

D

I

I

R

R

F

V

A

A

R

R

K

P

A

E

D

D

C

A

A

E

I

I

A

Q

E

I

I

I

Y

Y

N

A

H

P

A

I

V

V

L

L

Y

N

T

T

A

A

A

-

I

I

W

S

N

F

D

E

Q

E

T

A

V

V

D

P

A

S

D

V

N

E

R

Q

I

M

A

R

W

E

F

R

E

I

A

S

R

T

T

T

L

L

A

Q

G

T

Y

Y

P

P

V

Y

L

L

V

V

S

A

E

V

E

R

N

E

G

G

V

R

V

V

T

V

G

G

Y

Y

A

A

S

Y

F

A

G

S

D

Q

W

H

R

R

S

A

F
x
R

D

A

G

A

F
x
Y

R

R

H

W

T

A

V

V

E

D

H

V

S

T

V

V

Y

Y

V

V

H

A

P

E

D

G

H

Q

Q

R

G

R

K

S

G

G

L

I

G

A

R

R

K

Q

L

L

L

Y

S

D

R

V

L

L

I

L

D

P

E

V

A

L

R

K

D

R

C

L

G

G

K

Y

H

R

V

S

M

A

V

Y

A

A

G

G

I

I

E

A

S

L

Q

P

N

N

Q

E

A

G

S

S

L

V

H

G

L

L

H

H

Q

E

S

R

L

L

G

G

F

F

V

Q

V

H

T

I

A

G

Q

T

M

F

P

P

Q

Q

V

V

G

G

T

F

K

K

F

L

G

D

R

A

W

W

L

H

D

D

L

V

T

G

F

Y

M

W

Q

R

L

F

Q

D

L

F

-

G

D

D

E

E

R

G

T

L

E

H

P

P

D

E

A

A

binding phosphinothricin: R74 (≠ F74), Y77 (≠ F77)

5wphA Crystal structure of arsn, n-acetyltransferase with substrate ast from pseudomonas putida kt2440 (see paper)
33% identity, 99% coverage: 1:170/172 of query aligns to 2:171/179 of 5wphA

query
sites
5wphA

M

I

S

D

I

I

R

R

F

V

A

A

R

R

K

P

A

E

D

D

C

A

A

E

I

I

A

Q

E

I

I

I

Y

Y

N

A

H

P

A

I

V

V

L

L

Y

N

T

T

A

A

A

-

I

I

W
x
S

N
x
F

D

E

Q

E

T

A

V

V

D

P

A

S

D

V

N

E

R

Q

I

M

A

R

W

E

F

R

E

I

A

S

R

T

T

T

L

L

A

Q

G

T

Y

Y

P

P

V

Y

L

L

V

V

S

A

E

V

E

R

N

E

G

G

V

R

V

V

T

V

G

G

Y

Y

A

A

S

Y

F

A

G

S

D

Q

W

H

R
|
R

S

A

F
x
R

D

A

G

A

F
x
Y

R

R

H

W

T

A

V

V

E

D

H

V

S

T

V

V

Y

Y

V

V

H

A

P

E

D

G

H

Q

Q

R

G

R

K

S

G

G

L

I

G

A

R

R

K

Q

L

L

L

Y

S

D

R

V

L

L

I

L

D

P

E

V

A

L

R

K

D

R

C

L

G

G

K

Y

H

R

V

S

M

A

V

Y

A

A

G

G

I

I

E

A

S

L

Q

P

N

N

Q

E

A

G

S

S

L

V

H

G

L

L

H

H

Q

E

S

R

L

L

G

G

F

F

V

Q

V

H

T

I

A

G

Q

T

M

F

P

P

Q

Q

V

V

G

G

T

F

K

K

F

L

G

D

R

A

W

W

L

H

D

D

L

V

T

G

F

Y

M

W

Q

R

L

F

Q

D

L

F

-

G

D

D

E

E

R

G

T

L

E

H

P

P

D

E

A

A

binding (2S)-2-amino-4-[hydroxy(methyl)arsoryl]butanoic acid: S29 (≠ W29), F30 (≠ N30), R72 (= R72), R74 (≠ F74), Y77 (≠ F77)

6wqbA Crystal structure of vipf from legionella hackeliae in complex with acetyl-coa
32% identity, 51% coverage: 49:136/172 of query aligns to 187:269/290 of 6wqbA

query
sites
6wqbA

A

A

G

S

Y

Y

P

T

V

L

L

F

V

L

S

A

E

S

E

Y

N

N

G

H

V

I

T

V

G

G

Y

K

A

A

S

H

F

I

G

-

D

H

W

W

R

Q

S

S

F

-

D

-

G

-

F

-

R

K

H

E

T

A

V

I

E

F

H

S

S

D

V
x
I

Y
x
A

V
x
I

H

F

P

P

D

Q

H

Y

Q
|
Q

G
|
G

K

Q

G
|
G

L

W

G
|
G

R
x
G

K

E

L

L

S

S

R

Y

L

C

I

I

D

N

E

Q

A

A

R

L

D

T

C

S

G

G

K

K

H

N

V

K

M

L

V

M

A

L

G
x
D

I

V

E

E

S

T

Q

S

N

N

Q

Q

A
x
N

S

A

L

L

H

H

L

L

H
x
Y

Q

T

S

R

L

L

G

G

F

F

binding acetyl coenzyme *a: I218 (≠ V85), A219 (≠ Y86), I220 (≠ V87), Q225 (= Q92), G226 (= G93), G228 (= G95), G230 (= G97), G231 (≠ R98), D252 (≠ G119), N259 (≠ A126), Y264 (≠ H131)

Sites not aligning to the query:

binding acetyl coenzyme *a: 76, 77, 78, 83, 84, 86, 88, 89, 109, 112, 119, 125, 169, 170, 289

6wqcA Crystal structure of vipf from legionella hackeliae in complex with coa
32% identity, 51% coverage: 49:136/172 of query aligns to 189:271/293 of 6wqcA

query
sites
6wqcA

A

A

G

S

Y

Y

P

T

V

L

L

F

V

L

S

A

E

S

E

Y

N

N

G

H

V

I

T

V

G

G

Y

K

A

A

S

H

F

I

G

-

D

H

W

W

R

Q

S

S

F

-

D

-

G

-

F

-

R

K

H

E

T

A

V

I

E

F

H

S

S

D

V
x
I

Y
x
A

V
x
I

H

F

P
|
P

D

Q

H

Y

Q
|
Q

G

G

K

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G
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x
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binding coenzyme a: I220 (≠ V85), A221 (≠ Y86), I222 (≠ V87), P224 (= P89), Q227 (= Q92), G230 (= G95), G232 (= G97), G233 (≠ R98), A262 (≠ S127), H264 (= H129), L265 (= L130)

Sites not aligning to the query:

binding coenzyme a: 77, 78, 79, 84, 85, 87, 89, 90, 110, 113, 120, 124, 169, 170, 291

P0A944 [Ribosomal protein bS18]-alanine N-acetyltransferase; KAT; Peptidyl-lysine N-acetyltransferase; EC 2.3.1.266; EC 2.3.1.- from Escherichia coli (strain K12) (see paper)
42% identity, 31% coverage: 84:136/172 of query aligns to 68:120/148 of P0A944

query
sites
P0A944

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Y115 (≠ H131) mutation to A: Loss of activity.

Q8ZJW4 [Ribosomal protein bS18]-alanine N-acetyltransferase; EC 2.3.1.266 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
42% identity, 31% coverage: 84:136/172 of query aligns to 68:120/148 of Q8ZJW4

query
sites
Q8ZJW4

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IAV 69:71 (≠ VYV 85:87) binding
RRGLGR 77:82 (≠ GKGLGR 93:98) binding
N108 (= N124) binding

2cnsA Rimi - ribosomal s18 n-alpha-protein acetyltransferase in complex with acetylcoa. (see paper)
42% identity, 31% coverage: 84:136/172 of query aligns to 68:120/151 of 2cnsA

query
sites
2cnsA

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binding acetyl coenzyme *a: N68 (≠ S84), I69 (≠ V85), A70 (≠ Y86), V71 (= V87), Q76 (= Q92), R77 (≠ G93), G79 (= G95), G81 (= G97), R82 (= R98), E103 (≠ G119), N108 (= N124), A110 (= A126), L114 (= L130), Y115 (≠ H131)

Sites not aligning to the query:

binding acetyl coenzyme *a: 22

2cnmA Rimi - ribosomal s18 n-alpha-protein acetyltransferase in complex with a bisubstrate inhibitor (cterm-arg-arg-phe-tyr-arg-ala-n-alpha- acetyl-s-coa). (see paper)
42% identity, 31% coverage: 84:136/172 of query aligns to 68:120/151 of 2cnmA

query
sites
2cnmA

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binding coenzyme a: I69 (≠ V85), A70 (≠ Y86), V71 (= V87), Q76 (= Q92), R77 (≠ G93), G79 (= G95), G81 (= G97), R82 (= R98), V104 (≠ I120), A110 (= A126), L114 (= L130), Y115 (≠ H131)
binding : N68 (≠ S84), E103 (≠ G119)

Sites not aligning to the query:

binding coenzyme a: 22, 23
binding : 27, 28, 31, 67, 129, 130, 131

Query Sequence

>15569 FitnessBrowser__Keio:15569
MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFEARTLAGYPVLVSEENG
VVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLSRLIDEARDCGKHVMVAGI
ESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQLQLDERTEPDAIG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory