SitesBLAST – Find functional sites

 

SitesBLAST

Comparing 15854 b1736 N,N'-diacetylchitobiose-specific enzyme IIA component of PTS (NCBI) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

P69791 PTS system N,N'-diacetylchitobiose-specific EIIA component; EIIA-Chb; EIII-Chb; IIIcel; N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIA component from Escherichia coli (strain K12) (see paper)
100% identity, 100% coverage: 1:116/116 of query aligns to 1:116/116 of P69791

query
sites
P69791
M
 
M
M
 
M
D
 
D
L
 
L
D
 
D
N
 
N
I
 
I
P
 
P
D
 
D
T
 
T
Q
 
Q
T
 
T
E
 
E
A
 
A
E
 
E
E
 
E
L
 
L
E
 
E
E
 
E
V
 
V
V
 
V
M
 
M
G
 
G
L
 
L
I
 
I
I
 
I
N
 
N
S
 
S
G
 
G
Q
 
Q
A
 
A
R
 
R
S
 
S
L
 
L
A
 
A
Y
 
Y
A
 
A
A
 
A
L
 
L
K
 
K
Q
 
Q
A
 
A
K
 
K
Q
 
Q
G
 
G
D
 
D
F
 
F
A
 
A
A
 
A
A
 
A
K
 
K
A
 
A
M
 
M
M
 
M
D
 
D
Q
 
Q
S
 
S
R
 
R
M
 
M
A
 
A
L
 
L
N
 
N
E
 
E
A
 
A
H
 
H
L
 
L
V
 
V
Q
 
Q
T
 
T
K
 
K
L
 
L
I
 
I
E
 
E
G
 
G
D
 
D
A
 
A
G
 
G
E
 
E
G
 
G
K
 
K
M
 
M
K
 
K
V
 
V
S
 
S
L
 
L
V
 
V
L
 
L
V
 
V
H
 
H
A
 
A
Q
 
Q
D
|
D
H
 
H
L
 
L
M
 
M
T
 
T
S
 
S
M
 
M
L
 
L
A
 
A
R
 
R
E
 
E
L
 
L
I
 
I
T
 
T
E
 
E
L
 
L
I
 
I
E
 
E
L
 
L
H
 
H
E
 
E
K
 
K
L
 
L
K
 
K
A
 
A

2lrlA Solution structures of the iia(chitobiose)-hpr complex of the n,n'- diacetylchitobiose branch of the escherichia coli phosphotransferase system (see paper)
99% identity, 89% coverage: 14:116/116 of query aligns to 1:103/103 of 2lrlA

query
sites
2lrlA
A
 
A
E
 
E
E
 
E
L
 
L
E
 
E
E
 
E
V
 
V
V
 
V
M
 
M
G
 
G
L
 
L
I
 
I
I
 
I
N
 
N
S
 
S
G
 
G
Q
 
Q
A
 
A
R
 
R
S
 
S
L
 
L
A
 
A
Y
 
Y
A
 
A
A
 
A
L
 
L
K
 
K
Q
 
Q
A
 
A
K
 
K
Q
 
Q
G
 
G
D
 
D
F
 
F
A
 
A
A
 
A
A
 
A
K
 
K
A
 
A
M
 
M
M
 
M
D
 
D
Q
 
Q
S
 
S
R
 
R
M
 
M
A
 
A
L
 
L
N
 
N
E
 
E
A
 
A
H
 
H
L
 
L
V
 
V
Q
 
Q
T
 
T
K
 
K
L
 
L
I
 
I
E
 
E
G
 
G
D
 
D
A
 
A
G
 
G
E
 
E
G
 
G
K
 
K
M
 
M
K
 
K
V
 
V
S
 
S
L
 
L
V
 
V
L
 
L
V
 
V
H
|
H
A
 
A
Q
|
Q
D
 
L
H
|
H
L
 
L
M
 
M
T
 
T
S
 
S
M
 
M
L
 
L
A
 
A
R
 
R
E
 
E
L
 
L
I
 
I
T
 
T
E
 
E
L
 
L
I
 
I
E
 
E
L
 
L
H
 
H
E
 
E
K
 
K
L
 
L
K
 
K
A
 
A

P0A0D6 PTS system lactose-specific EIIA component; EIIA-Lac; EIII-Lac; Lactose-specific phosphotransferase enzyme IIA component from Staphylococcus aureus (see 2 papers)
34% identity, 85% coverage: 15:113/116 of query aligns to 4:102/103 of P0A0D6

query
sites
P0A0D6
E
 
E
E
 
E
L
 
V
E
 
Q
E
 
L
V
 
L
V
 
G
M
 
F
G
 
E
L
 
I
I
 
V
I
 
A
N
 
F
S
 
A
G
 
G
Q
 
D
A
 
A
R
 
R
S
 
S
L
 
K
A
 
F
Y
 
L
A
 
E
A
 
A
L
 
L
K
 
T
Q
 
A
A
 
A
K
 
Q
Q
 
A
G
 
G
D
 
D
F
 
F
A
 
A
A
 
K
A
 
A
K
 
D
A
 
A
M
 
L
M
 
I
D
 
E
Q
 
E
S
 
G
R
 
N
M
 
N
A
 
C
L
 
I
N
 
A
E
 
E
A
 
A
H
 
H
L
 
R
V
 
A
Q
 
Q
T
 
T
K
 
S
L
 
L
I
 
L
E
 
A
G
 
K
D
 
E
A
 
A
G
 
Q
E
 
G
G
 
D
K
 
D
M
 
I
K
 
A
V
 
Y
S
 
S
L
 
V
V
 
T
L
 
M
V
 
M
H
|
H
A
 
G
Q
 
Q
D
 
D
H
|
H
L
 
L
M
 
M
T
 
T
S
 
T
M
 
I
L
 
L
A
 
L
R
 
K
E
 
D
L
 
L
I
 
M
T
 
K
E
 
H
L
 
L
I
 
L
E
 
E
L
 
F
H
 
Y
E
 
K
K
 
R

P23532 PTS system lactose-specific EIIA component; EIIA-Lac; EIII-Lac; Lactose-specific phosphotransferase enzyme IIA component from Lactococcus lactis subsp. lactis (Streptococcus lactis) (see 2 papers)
35% identity, 85% coverage: 15:113/116 of query aligns to 4:102/105 of P23532

query
sites
P23532
E
 
E
E
 
E
L
 
M
E
 
T
E
 
L
V
 
L
V
 
G
M
 
F
G
 
E
L
 
I
I
 
V
I
 
A
N
 
Y
S
 
A
G
|
G
Q
 
D
A
 
A
R
 
R
S
 
S
L
 
K
A
 
L
Y
 
L
A
 
E
A
 
A
L
 
L
K
 
K
Q
 
A
A
 
A
K
 
E
Q
 
N
G
 
G
D
 
D
F
 
F
A
 
A
A
 
K
A
 
A
K
 
D
A
 
S
M
 
L
M
 
V
D
 
V
Q
 
E
S
 
A
R
 
G
M
 
S
A
 
C
L
 
I
N
 
A
E
 
E
A
 
A
H
 
H
L
 
S
V
 
S
Q
 
Q
T
 
T
K
 
G
L
 
M
I
 
L
E
 
A
G
 
R
D
 
E
A
 
A
G
 
S
E
 
G
G
 
E
K
 
E
M
 
L
K
 
P
V
 
Y
S
 
S
L
 
V
V
 
T
L
 
M
V
 
M
H
 
H
A
 
G
Q
 
Q
D
|
D
H
 
H
L
 
L
M
 
M
T
 
T
S
 
T
M
 
I
L
 
L
A
 
L
R
 
K
E
 
D
L
 
V
I
 
I
T
 
H
E
 
H
L
 
L
I
 
I
E
 
E
L
 
L
H
 
Y
E
 
K
K
 
R

Query Sequence

>15854 b1736 N,N'-diacetylchitobiose-specific enzyme IIA component of PTS (NCBI)
MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQGDFAAAKAMMDQSRMA
LNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARELITELIELHEKLKA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory