SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 15856 FitnessBrowser__Keio:15856 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

P69795 PTS system N,N'-diacetylchitobiose-specific EIIB component; EIIB-Chb; IVcel; N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component; EC 2.7.1.196 from Escherichia coli (strain K12) (see 2 papers)
100% identity, 100% coverage: 1:106/106 of query aligns to 1:106/106 of P69795

query
sites
P69795

M

M

E

E

K

K

H

H

I

I

Y

Y

L

L

F

F

C
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C

S

S

A

A

G

G

M

M

S

S

T

T

S

S

L

L

V

V

S

S

K

K

M

M

R

R

A

A

Q

Q

A

A

E

E

K

K

Y

Y

E

E

V

V

P

P

V

V

I

I

E

E

A

A

F

F

P

P

E

E

T

T

L

L

A

A

G

G

E

E

K

K

G

G

Q

Q

N

N

A

A

D

D

V

V

L

L

G

G

P

P

Q

Q

I

I

A

A

Y

Y

M

M

L

L

P

P

E

E

I

I

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Q

R

R

L

L

P

P

N

N

K

K

P

P

V

V

E

E

V

V

I

I

D

D

S

S

L

L

Y

Y

G

G

K

K

V

V

D

D

G

G

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L

G

G

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V

L

L

K

K

A

A

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V

A

A

I

I

K

K

A

A

N

N

C10 (= C10) active site, Phosphocysteine intermediate; mutation to S: Unable to be phosphorylated by EIIA.

2wy2D Nmr structure of the iiachitobiose-iibchitobiose phosphoryl transition state complex of the n,n'-diacetylchitoboise brance of the e. Coli phosphotransferase system. (see paper)
100% identity, 97% coverage: 3:105/106 of query aligns to 1:103/103 of 2wy2D

query
sites
2wy2D

K

K

H

H

I

I

Y

Y

L

L

F

F

C
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C

S
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S

A
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A

G
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G

M
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M

S
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S

T

T

S

S

L

L

V

V

S

S

K

K

M

M

R

R

A

A

Q

Q

A

A

E

E

K

K

Y

Y

E

E

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V

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P

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I

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E

A

A

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F

P

P

E

E

T

T

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L

A

A

G

G

E

E

K

K

G

G

Q

Q

N

N

A

A

D

D

V

V

L

L

G

G

P

P

Q

Q

I

I

A

A

Y

Y

M

M

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L

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P

E

E

I

I

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Q

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R

L

L

P

P

N

N

K

K

P

P

V

V

E

E

V

V

I

I

D

D

S

S

L

L

Y

Y

G

G

K

K

V

V

D

D

G

G

L

L

G

G

V

V

L

L

K

K

A

A

V

V

A

A

I

I

K

K

A

A

binding phosphite ion: C8 (= C10), S9 (= S11), A10 (= A12), G11 (= G13), M12 (= M14), S13 (= S15)

P46318 Lichenan-specific phosphotransferase enzyme IIB component; EIIB-Lic; PTS system lichenan-specific EIIB component; EC 2.7.1.- from Bacillus subtilis (strain 168) (see paper)
47% identity, 87% coverage: 5:96/106 of query aligns to 2:95/102 of P46318

query
sites
P46318

H

N

I

I

Y

L

L

L

F

V

C

C

S

A

A

A

G

G

M

M

S

S

T

T

S

S

L

L

V

V

S

S

K

K

M

M

R

E

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K

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S

A

A

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K

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Y

Q

E

G

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D

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Y

I

T

I

I

E

W

A

A

F

-

P

-

E

-

T

-

L

V

A
x
S

G

G

E

D

K

S

G

V

Q

Q

N

N

-

H

-

I

-

D

-

K

A

A

D

D

V

V

V

L

L

L

G

G

P

P

Q

Q

I

V

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R

Y

Y

M

M

L

L

P

P

E

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I

L

Q

K

R

K

L

L

L

G

P

E

N

S

K

K

-

G

-

V

P

P

V

V

E

D

V

V

I

I

D

N

S

T

L

V

L

H

Y

Y

G

G

K

T

V

C

D

N

G

G

L

A

G

E

V

V

L

L

K

K

A

S

A

A

S37 (≠ A44) modified: Phosphoserine

Query Sequence

>15856 FitnessBrowser__Keio:15856
MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLAGEKGQNADVVLLGPQI
AYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKKAAAN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory