SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 16133 b2025 imidazole glycerol phosphate synthase subunit HisF (NCBI) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P60664 Imidazole glycerol phosphate synthase subunit HisF; IGP synthase cyclase subunit; IGP synthase subunit HisF; ImGP synthase subunit HisF; IGPS subunit HisF; EC 4.3.2.10 from Escherichia coli (strain K12) (see paper)
100% identity, 100% coverage: 1:258/258 of query aligns to 1:258/258 of P60664

query
sites
P60664

M

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C

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I

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Y

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D

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G

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N

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Y

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G

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L

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N

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R

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N

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Y

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D

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C

R5 (= R5) mutation to A: Loss of activity.; mutation to H: Loss of IGP synthase activity. Weak IGP synthase activity and reduced HisH activity in vitro.
E46 (= E46) mutation to A: Loss of activity.; mutation to G: Loss of IGP synthase activity. Weak IGP synthase and HisH activities in vitro.
Q123 (= Q123) mutation to A: Decrease in activity.; mutation to R: Loss of IGP synthase activity. Weak HisH activity in vitro.
C124 (= C124) mutation to A: No change in activity.; mutation to R: Loss of IGP synthase activity. Weak HisH activity in vitro.

Q9X0C6 Imidazole glycerol phosphate synthase subunit HisF; IGP synthase cyclase subunit; IGP synthase subunit HisF; ImGP synthase subunit HisF; IGPS subunit HisF; EC 4.3.2.10 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
51% identity, 100% coverage: 1:257/258 of query aligns to 1:250/253 of Q9X0C6

query
sites
Q9X0C6

M

M

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L

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C9 (= C9) mutation to A: No change in activity.
D11 (= D11) mutation to X: Loss of activity.
K19 (= K19) mutation to S: Decrease in activity.
D51 (= D51) mutation to N: No change in activity.
N103 (= N103) mutation to A: No change in activity.
D130 (= D130) mutation D->A,C,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y: Complete loss of activity.; mutation to E: Weak activity.
D176 (= D182) mutation to N: Decrease in activity.
D183 (= D189) mutation to N: No change in activity.

7ac8A Molecular basis for the unique allosteric activation mechanism of the heterodimeric imidazole glycerol phosphate synthase complex. (see paper)
51% identity, 100% coverage: 1:257/258 of query aligns to 1:250/252 of 7ac8A

query
sites
7ac8A

M

M

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L

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active site: D11 (= D11), D130 (= D130)
binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: L50 (≠ Y50), I52 (= I52), G82 (= G82), N103 (= N103), T104 (≠ S104), D130 (= D130), S144 (≠ T146), D176 (= D182), G177 (= G183), S201 (= S207), S225 (= S232)

1gpwC Structural evidence for ammonia tunneling across the (beta/alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex. (see paper)
51% identity, 100% coverage: 1:257/258 of query aligns to 1:250/253 of 1gpwC

query
sites
1gpwC

M

M

L

L

A

A

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K

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C

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N

V

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D

D

G

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K

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N

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G

A

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D

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S
x
T

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active site: N11 (≠ D11), D130 (= D130)
binding phosphate ion: G82 (= G82), N103 (= N103), T104 (≠ S104), D176 (= D182), G177 (= G183), S225 (= S232)

7qc8A Imidazole glycerol phosphate synthase subunit HisF (see paper)
50% identity, 99% coverage: 2:257/258 of query aligns to 2:250/250 of 7qc8A

query
sites
7qc8A

L

L

A

A

K

K

R

R

I

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P

A

C

A

L

L

D
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E

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V

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K

D

D

G

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K

G

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H

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K

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V

E

E

V

V

Q

E

K

K

R

R

G

G

A

A

G

G

E

E

I

I

V

L

L

L

N

T

M

S

M

I

N

D

Q

R

D

D

G

G

V

T

R

K

N

S

G

G

Y

Y

D

D

L

T

E

E

Q

M

L

I

K

R

K

F

V

V

R

R

E

P

V

L

C

T

H

T

V

L

P

P

L

I

I

I

A

A

S

S

G

G

A

A

G

G

T

K

M

M

E

E

H

H

F

F

L

L

E

E

A

A

F

-

R

F

D

L

A

A

D

G

V

A

D

D

G

A

A

A

L

L

A

A

S

S

V

V

F

F

H

H

K

F

Q

R

I

E

I

I

N

D

I

V

G

R

E

E

L

L

K

K

A

E

Y

Y

L

L

A

K

T

K

Q

H

G

G

V

V

E

N

I

V

R

R

I

L

binding zinc ion: E11 (≠ D11), H50 (≠ Y50), H52 (≠ I52)

3zr4E Structural evidence for ammonia tunneling across the (beta-alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex (see paper)
50% identity, 100% coverage: 1:257/258 of query aligns to 1:241/244 of 3zr4E

query
sites
3zr4E

M

M

L

L

A

A

K

K

R

R

I

I

P

A

C

C

L

L

D
|
D

V

V

R

K

D

D

G

G

Q

R

V

V

K

K

G

S

V

-

Q

-

F

-

R

-

N

-

H

-

E

-

I

-

G

G

D

D

I

P

V

V

P

E

L

L

A

G

K

K

R

F

Y

Y

A

S

E

E

E

I

G

G

A

I

D

D

E

E

L

L

V

V

F

F

Y

L

D

D

I

I

T

T

A

A

S

S

S

V

D

E

G

K

R

R

V

K

V

T

D

M

K

L

S

E

W

L

V

V

S

E

R

K

V

V

A

A

E

E

V

Q

I

I

D

D

I

I

P

P

F

F

C

T

V

V

A

G

G

G

I

I

K

H

S

D

L

F

E

E

D

T

A

A

A

S

K

E

I

L

L

I

S

L

F

R

G

G

A

A

D

D

K

K

I

V

S

S

I

I

N

N

S

T

P

A

A

A

L

V

A

E

D

N

P

P

T

S

L

L

I

I

T

T

R

Q

L

I

A

A

D

Q

R

T

F

F

G

G

V

S

Q

Q

C

A

I

V

V

V

G

A

I

I

D
|
D

T

A

W

-

Y

-

D

K

A

R

E

V

T

D

G

G

K

E

Y

F

H

M

V

V

N

F

Q

T

Y

Y

T

S

G

G

D

K

E

K

S

N

R

-

T

-

R

-

V

-

T

T

Q

G

W

I

E

L

T

L

L

R

D

D

W

W

V

V

Q

V

E

E

V

V

Q

E

K

K

R

R

G

G

A

A

G

G

E

E

I

I

V

L

L

L

N

T

M

S

M

I

N

D

Q

R

D

D

G

G

V

T

R

K

N

S

G

G

Y

Y

D

D

L

T

E

E

Q

M

L

I

K
x
R

K

F

V

V

R

R

E

P

V

L

C

T

H

T

V

L

P

P

L

I

I

I

A

A

S

S

G

G

A

A

G

G

T

K

M

M

E

E

H

H

F

F

L

L

E

E

A

A

F

F

R
x
L

D

A

A

G

D

A

V

D

D

A

G

-

A

A

L

L

A

A

S

S

V

V

F

F

H

H

K

F

Q

R

I

E

I

I

N

D

I

V

G

R

E

E

L

L

K

K

A

E

Y

Y

L

L

A

K

T

K

Q

H

G

G

V

V

E

N

I

V

R

R

I

L

active site: D11 (= D11), D121 (= D130)
binding glycerol: R179 (≠ K194), L206 (≠ R221)

5d2tA Directed evolutionary changes in kemp eliminase ke07 - crystal 3 wild type
48% identity, 99% coverage: 3:257/258 of query aligns to 1:248/251 of 5d2tA

query
sites
5d2tA

A

A

K

K

R

R

I

I

P

A

C

A

L

L

D

I

V

V

R

K

D

D

G

G

Q

R

V

V

K

K

G

G

V

S

Q

N

F

F

R

E

N

N

H

L

E

R

I

D

I

S

G

G

D

D

I

P

V

V

P

E

L

L

A

G

K

K

R

F

Y

Y

A

S

E

E

E

I

G

G

A

I

D

D

E

E

L

L

V

S

F

F

Y
x
W

D

D

I

I

T

T

A

A

S

S

S

V

D

E

G

K

R

R

V

K

V

T

D

M

K

L

S

E

W

L

V

V

S

E

R

K

V

V

A

A

E

E

V

Q

I

I

D

D

I

I

P

P

F

F

C

T

V

V

A

G

G

G

I

I

K

H

S

D

L

F

E

E

D

T

A

A

A

S

K

E

I

L

L

I

S

L

F

R

G

G

A

A

D

D

K

K

I

V

S
x
E

I

I

N

N

S

T

P

A

A

A

L

V

A

E

D

N

P

P

T

S

L

L

I

I

T

T

R

Q

L

I

A

A

D

Q

R

T

F

F

G

G

V

S

Q

Q

C

A

I

V

V

V

G
x
Y

I

I

D

A

T

A

W

-

Y

-

D

K

A

R

E

V

T

D

G

G

K

E

Y

F

H

M

V

V

N

F

Q

T

Y

Y

T

S

G

G

D

K

E

K

S

N

R

-

T

-

R

-

V

-

T

T

Q

G

W

I

E

L

T

L

L

R

D

D

W

W

V

V

Q

V

E

E

V

V

Q

E

K

K

R

R

G

G

A

A

G

G

E

E

I

I

V

V

L

L

N

G

M

S

M

I

N

D

Q

R

D

L

G

G

V

T

R

K

N

S

G

G

Y

Y

D

D

L

T

E

E

Q

M

L

I

K

R

K

F

V

V

R

R

E

P

V

L

C

T

H

T

V

L

P

P

L

I

I

I

A

A

S

H

G

G

A

A

G

G

T

K

M

M

E

E

H

H

F

F

L

L

E

E

A

A

F

F

R

L

D

-

A

A

D

G

V

A

D

D

G

A

A

A

L
x
K

A

A

A

N

S

S

V

V

F

L

H

H

K

F

Q

R

I

E

I

I

N

D

I

V

G

R

E

E

L

L

K

K

A

E

Y

Y

L

L

A

K

T

K

Q

H

G

G

V

V

E

N

I

V

R

R

I

L

binding 5-nitro-2-oxidanyl-benzenecarbonitrile: W48 (≠ Y50), E99 (≠ S101), Y126 (≠ G128), K220 (≠ L229)

6dnjA Directed evolutionary changes in kemp eliminase ke07 - crystal 28 round 5 (see paper)
48% identity, 99% coverage: 2:257/258 of query aligns to 1:249/250 of 6dnjA

query
sites
6dnjA

L

L

A

A

K

K

R

R

I

I

I

D

P

A

C

A

L

L

D

I

V

M

R

K

D

D

G

G

Q

R

V

V

K

K

G

G

V

S

Q

N

F

F

R

E

N

N

H

L

E

R

I

D

I

S

G

G

D

D

I

P

V

V

P

E

L

L

A

G

K

K

R

F

Y

Y

A

S

E

E

E

I

G

G

A

I

D

D

E

E

L

L

V

S

F

F

Y
x
W

D

D

I

I

T

T

A

A

S

S

S

V

D

E

G

K

R

R

V

K

V

T

D

M

K

L

S

E

W

L

V

V

S

E

R

K

V

V

A

A

E

E

V

Q

I

I

D

D

I

I

P

P

F

F

C

T

V

V

A
x
G

G
|
G

G

G

I

I

K

H

S

D

L

F

E

E

D

T

A

A

A

S

K

E

I

L

L

I

S

L

F

R

G

G

A

A

D

D

K

K

I

V

S

E

I

I

N
|
N

S

T

P

A

A

A

L

V

A

E

D

N

P

P

T

S

L

L

I

I

T

T

R

Q

L

I

A

A

D

Q

R

T

F

F

G

G

V

S

Q

Q

C

A

I

V

V

V

G
x
Y

I

I

D

A

T

A

W

-

Y

-

D

K

A

R

E

V

T

D

G

G

K

E

Y

F

H

M

V

V

N

F

Q

T

Y

Y

T

S

G

G

D

K

E

K

S

N

R

-

T

-

R

-

V

-

T

T

Q

G

W

I

E

L

T

L

L

R

D

D

W

W

V

V

Q

V

E

E

V

V

Q

E

K

K

R

R

G

G

A

A

G

G

E

E

I

I

V

V

L

L

N

G

M

S

M

I

N

D

Q

R

D

L

G

G

V

T

R

K

N

S

G

G

Y

Y

D

D

L

T

E

E

Q

M

L

I

K

R

K

F

V

V

R

R

E

P

V

L

C

T

H

T

V

L

P

P

L

I

I

I

A

A

S

H

G

R

G

G

A

A

G

G

T

K

M

M

E

E

H

H

F

F

L

L

E

E

A

A

F

F

R

L

D

-

A

A

D

G

V

A

D

D

G

A

A

A

L

K

A

A

A

D

S

S

V

V

F

F

H

H

K

F

Q

R

I

E

I

I

N

D

I

V

G

R

E

E

L

L

K

K

A

E

Y

Y

L

L

A

K

T

K

Q

H

G

G

V

V

E

N

I

V

R

R

I

L

binding 5-nitro-1,2-benzoxazole: W49 (≠ Y50), G79 (≠ A80), G80 (= G81), N102 (= N103), Y127 (≠ G128)

4ewnD Structure of hisf-d130v+d176v with bound rcdrp (see paper)
50% identity, 99% coverage: 2:257/258 of query aligns to 1:243/243 of 4ewnD

query
sites
4ewnD

L

L

A

A

K

K

R

R

I

I

P

A

C

C

L

L

D

D

V

V

R

K

D

D

G

G

Q

R

V

V

K

K

G

G

V

T

Q

N

F

F

R

E

N

N

H

L

E

R

I

D

I

S

G

G

D

D

I

P

V

V

P

E

L

L

A

G

K

K

R

F

Y

Y

A

S

E

E

E

I

G

G

A

I

D

D

E

E

L

L

V

V

F

F

Y

L

D

D

I

I

T

T

A

K

S

T

S

M

D

-

G

-

R

-

V

-

D

-

K

L

S

E

W

L

V

V

S

E

R

K

V

V

A

A

E

E

V

Q

I

I

D

D

I

I

P

P

F

F

C

T

V

V

A

G

G

G

I

I

K

H

S

D

L

F

E

E

D

T

A

A

A

S

K

E

I

L

L

I

S

L

F

R

G

G

A

A

D

D

K

K

I

V

S

S

I

I

N

N

S

T

P

A

A

A

L

V

A

E

D

N

P

P

T

S

L

L

I

I

T

T

R

Q

L

I

A

A

D

Q

R

T

F

F

G

G

V

S

Q

Q

C

A

I

V

V

V

G

A

I

I

D

-

T

-

W
x
V

Y

A

D

K

A

R

E

V

T

D

G

G

K

E

Y

F

H

M

V

V

N

F

Q

T

Y

Y

T

S

G

G

D

K

E

K

S

N

R

-

T

-

R

-

V

-

T

T

Q

G

W

I

E

L

T

L

L

R

D

D

W

W

V

V

Q

V

E

E

V

V

Q

E

K

K

R

R

G

G

A

A

G

G

E

E

I

I

V

L

L

L

N

T

M

S

M

I

N

D

Q

R

D

V

G
|
G

V

T

R

K

N

S

G

G

Y

Y

D

D

L

T

E

E

Q

M

L

I

K

R

K

F

V

V

R

R

E

P

V

L

C

T

H

T

V

L

P

P

L

I

I

I

A

A

S
|
S

G
|
G

A

A

G

G

T

K

M

M

E

E

H

H

F

F

L

L

E

E

A

A

F

-

R

F

D

L

A

A

D

G

V

A

D

D

G

A

A

A

L
|
L

A
|
A

S
|
S

V

V

F

F

H

H

K

F

Q

R

I

E

I

I

N

D

I

V

G

R

E

E

L

L

K

K

A

E

Y

Y

L

L

A

K

T

K

Q

H

G

G

V

V

E

N

I

V

R

R

I

L

binding 1-(O-carboxy-phenylamino)-1-deoxy-D-ribulose-5-phosphate: V123 (≠ W132), G170 (= G183), S194 (= S207), G195 (= G208), G196 (= G209), L215 (= L229), A216 (= A230), A217 (= A231), S218 (= S232)

2wjzE Crystal structure of (hish) k181a y138a mutant of imidazoleglycerolphosphate synthase (hish hisf) which displays constitutive glutaminase activity (see paper)
50% identity, 100% coverage: 1:257/258 of query aligns to 1:236/237 of 2wjzE

query
sites
2wjzE

M

M

L

L

A

A

K

K

R

R

I

I

P

A

C

C

L

L

D
|
D

V

V

R

K

D

D

G

G

Q

R

V

V

K

-

G

-

V

-

Q

-

F

-

R

-

N

-

H

-

E

-

I

-

G

G

D

D

I

P

V

V

P

E

L

L

A

G

K

K

R

F

Y

Y

A

S

E

E

E

I

G

G

A

I

D

D

E

E

L

L

V

V

F

F

Y

L

D

D

I

I

T

T

A

A

S

K

S

-

D

-

G

-

R

R

V

K

V

T

D

M

K

L

S

E

W

L

V

V

S

E

R

K

V

V

A

A

E

E

V

Q

I

I

D

D

I

I

P

P

F

F

C

T

V

V

A

G

G

G

G
|
G

I

I

K

H

S

D

L

F

E

E

D

T

A

A

A

S

K

E

I

L

L

I

S

L

F

R

G

G

A

A

D

D

K

K

I

V

S

S

I

I

N
|
N

S
x
T

P

A

A

A

L

V

A

E

D

N

P

P

T

S

L

L

I

I

T

T

R

Q

L

I

A

A

D

Q

R

T

F

F

G

G

V

S

Q

Q

C

A

I

V

V

V

G

A

I

I

D
|
D

T

A

W

-

Y

-

D

K

A

R

E

V

T

D

G

G

K

E

Y

F

H

M

V

V

N

F

Q

T

Y

Y

T

S

G

G

D

K

E

K

S

N

R

-

T

-

R

-

V

-

T

T

Q

G

W

I

E

L

T

L

L

R

D

D

W

W

V

V

Q

V

E

E

V

V

Q

E

K

K

R

R

G

G

A

A

G

G

E

E

I

I

V

L

L

L

N

T

M

S

M

I

N

D

Q

R

D

D

G

G

V

T

R

K

N

S

G

G

Y

Y

D

D

L

T

E

E

Q

M

L

I

K

R

K

F

V

V

R

R

E

P

V

L

C

T

H

T

V

L

P

P

L

I

I

I

A

A

S

S

G

G

A

A

G

G

T

K

M

M

E

E

H

H

F

F

L

L

E

E

A

A

F

-

R

F

D

L

A

A

D

G

V

A

D

D

G

A

A

A

L

L

A

A

S

S

V

V

F

F

H

H

K

F

Q

R

I

E

I

I

N

D

I

V

G

R

E

E

L

L

K

K

A

E

Y

Y

L

L

A

K

T

K

Q

H

G

G

V

V

E

N

I

V

R

R

I

L

active site: D11 (= D11), D116 (= D130)
binding phosphate ion: G68 (= G82), N89 (= N103), T90 (≠ S104)

1h5yB Hisf protein from pyrobaculum aerophilum (see paper)
45% identity, 99% coverage: 2:257/258 of query aligns to 3:253/253 of 1h5yB

query
sites
1h5yB

L

M

A

A

K

L

R

R

I

I

P

P

C

C

L

L

D
|
D

V

I

R

D

D

G

G

G

Q

A

-

K

-

V

V

V

K
|
K

G

G

V

V

Q

N

F

F

R

Q

N

G

H

I

E

R

I

E

I

V

G

G

D

D

I

P

V

V

P

E

L

M

A

A

K

V

R

R

Y

Y

A

E

E

E

G

G

A

A

D

D

E

E

L

I

V

A

F

I

Y

L

D

D

I

I

T

T

A

A

S

A

S

P

D

E

G

G

R

R

V

A

V

T

D

F

K

I

S

D

W

S

V

V

S

K

R

R

V

V

A

A

E

E

V

A

I

V

D

S

I

I

P

P

F

V

C

L

V

V

A

G

G
|
G

I

V

K

R

S

S

L

L

E

E

D

D

A

A

A

T

K

T

I

L

L

F

S

R

F

A

G

G

A

A

D

D

K

K

I

V

S

S

I

V

N
|
N

S
x
T

P

A

A

A

L

V

A

R

D

N

P

P

T

Q

L

L

I

V

T

A

R

L

L

L

A

A

D

R

R

E

F

F

G

G

V

S

Q

Q

C

S

I

T

V

V

G
x
A

I

I

D
|
D

T

A

W

K

Y

W

D

N

A

G

E

E

T

Y

G

-

K

-

Y

Y

H

E

V

V

N

Y

Q

V

Y

K

T
x
G

G

G

D

R

E

E

S

A

R

-

T

-

R

-

V

-

T

T

Q

G

W

L

E

D

T

A

L

V

D

K

W

W

V

A

Q

K

E

E

V

V

Q

E

K

E

R

L

G

G

A

A

G

G

E

E

I

I

V

L

L

L

N
x
T

M

S

M

I

N

D

Q

R

D
|
D

G
|
G

V

T

R

G

N

L

G

G

Y

Y

D

D

L

V

E

E

Q

L

L

I

K

R

V

V

R

A

E

D

V

S

C

V

H

R

V

I

P

P

L

V

I

I

A

A

S

S

G

G

A

A

G

G

T

R

M

V

E

E

H

H

F

F

L

Y

E

E

A

A

F

A

R

A

D

A

A

G

D

A

V

D

D

A

G

-

A

V

L

L

A
|
A

S
|
S

V

L

F

F

H

H

K

F

Q

R

I

V

I

L

N

S

I

I

G

A

E

Q

L

V

K

K

A

R

Y

Y

L

L

A

K

T

E

Q

R

G

G

V

V

E

E

I

V

R

R

I

I

active site: D12 (= D11), D133 (= D130)
binding glycerol: K22 (= K19), N106 (= N103), A131 (≠ G128), D133 (= D130), G147 (≠ T146), T174 (≠ N177), D179 (= D182)
binding phosphate ion: G84 (= G81), G85 (= G82), N106 (= N103), T107 (≠ S104), D179 (= D182), G180 (= G183), A226 (= A230), A227 (= A231), S228 (= S232)

3iivB Evolutionary optimization of computationally designed enzymes: kemp eliminases of the ke07 series (see paper)
47% identity, 99% coverage: 2:257/258 of query aligns to 2:249/262 of 3iivB

query
sites
3iivB

L

L

A

A

K

K

R

R

I

I

I

D

P

A

C

A

L

L

D

I

V

L

R

K

D

D

G

G

Q

R

V

V

K

K

G

G

V

S

Q

N

F

F

R

E

N

N

H

L

E

R

I

D

I

S

G

G

D

D

I

P

V

V

P

E

L

L

A

G

K

K

R

F

Y

Y

A

S

E

E

E

I

G

G

A

I

D

D

E

E

L

L

V

S

F

F

Y

W

D

D

I

I

T

T

A

A

S

S

S

V

D

E

G

K

R

K

V

T

V

M

D

-

K

L

S

E

W

L

V

V

S

E

R

K

V

V

A

A

E

E

V

Q

I

I

D

D

I

I

P

P

F

I

C

T

V

V

A

G

G

G

I

I

K

Y

S

D

L

F

E

E

D

T

A

A

A

S

K

E

I

L

L

I

S

L

F

R

G

G

A

A

D

D

K

K

I

V

S
x
E

I

I

N

N

S

T

P

A

A

A

L

V

A

E

D

N

P

P

T

S

L

L

I

I

T

T

R

Q

L

I

A

A

D

Q

R

T

F

F

G

G

V

S

Q

Q

C

A

I

V

V

V

G

Y

I

I

D

A

T

A

W

-

Y

-

D

K

A

R

E

V

T

D

G

G

K

E

Y

F

H

M

V

V

N

F

Q

T

Y

Y

T

S

G

G

D

-

E

-

S

-

R

-

T

T

R

K

V

N

T

T

Q

G

W

I

E

L

T

L

L

R

D

D

W

W

V

V

Q

V

E

E

V

V

Q

E

K

K

R

R

G

G

A

A

G

G

E

E

I

I

V

V

L

L

N

G

M

S

M

I

N

D

Q

R

D

L

G

G

V

T

R

K

N

S

G

G

Y

Y

D

D

L

T

E

E

Q

M

L

I

K

R

K

F

V

V

R

R

E

P

V

L

C

T

H

T

V

L

P

P

L

I

I

I

A

A

S

H

G

R

G

G

A

A

G

G

T

K

M

T

E

E

H

H

F

F

L

L

E

E

A

A

F

F

R

L

D

-

A

A

D

G

V

A

D

D

G

A

A

A

L
x
K

A

A

A

D

S

S

V

V

F

F

H

H

K

S

Q

R

I

E

I

I

N

D

I

V

G

R

E

E

L

L

K

K

A

E

Y

Y

L

L

A

K

T

K

Q

H

G

G

V

V

E

N

I

V

R

R

I

L

binding magnesium ion: E100 (≠ S101), K221 (≠ L229)

P33734 Imidazole glycerol phosphate synthase hisHF; IGP synthase; IGPS; ImGP synthase; EC 4.3.2.10; EC 3.5.1.2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
31% identity, 99% coverage: 2:257/258 of query aligns to 236:549/552 of P33734

query
sites
P33734

L

L

A

T

K

R

R
|
R

I

I

P

A

C

C

L

L

D

D

V

V

R

R

-

T

-

N

-

D

-

Q

-

G

D

D

G

L

Q

V

V

V

V

T

K
|
K

G

G

V

D

Q

Q

F

Y

R

D

N

V

H

R

E

E

I

K

-

S

-

D

-

G

-

K

-

G

-

V

-

R

-

N

I

L

G

G

D

K

I

P

V

V

P

Q

L

L

A

A

K

Q

R

K

Y

Y

A

Y

E

Q

G

G

A

A

D

D

E

E

L

V

V

T

F

F

Y

L

D

N

I

I

T

T

A

S

S

F

S

R

D

D

G

C

R

P

V

L

V

K

D

D

K

T

S

P

W

M

V

L

-

E

-

V

-

L

S

K

R

Q

V

A

A

A

E

K

V

T

I

V

D

F

I

V

P

P

F

L

C

T

V

V

A

G

G

G

I

I

K

K

-

D

-

I

-

V

-

D

-

V

-

D

-

G

-

T

-

K

-

I

-

P

S

A

L

L

E

E

D

V

A

A

A

S

K

L

I

Y

L

F

S

R

F

S

G

G

A

A

D

D

K
|
K

I

V

S

S

I

I

N

G

S

T

P

D

A

A

L

V

A

Y

D

A

P

A

T

E

L

K

I

Y

T

Y

R

E

L

L

A

G

D

N

R

R

-

G

-

D

-

G

-

T

-

S

-

P

-

I

-

E

-

T

-

I

-

S

-

K

-

A

F

Y

G

G

V

A

Q

Q

C

A

I

V

V

V

V

I

G

S

I

V

D

D

-

P

-

K

-

R

-

V

-

Y

-

V

-

N

-

S

-

Q

-

A

-

D

-

T

-

K

-

N

-

K

-

V

-

F

-

E

T

T

W

E

Y

Y

D

P

A

G

E

P

T

N

G

G

K

E

-

K

-

Y

-

C

-

W

Y

Y

H

Q

V

C

N

T

Q

I

Y

K

T

G

G

G

D

R

E

E

S

S

R

R

T

D

R

-

V

L

T

G

Q

V

W

W

E

E

T

-

L

-

D

-

W

L

V

T

Q

R

E

A

V

C

Q

E

K

A

R

L

G

G

A

A

G

G

E

E

I

I

V

L

L

L

N

N

M

C

M

I

N

D

Q

K

D

D

G

G

V

S

R

N

N

S

G

G

Y

Y

D

D

L

L

E

E

Q

L

L

I

K

E

K

H

V

V

R

K

E

D

V

A

C

V

H

K

V

I

P

P

L

V

I

I

A

A

S

S

G

S

G

G

A

A

G

G

T

V

M

P

E

E

H

H

F

F

L

E

E

E

A

A

F

F

R

L

D

K

A

T

D

R

V

A

D

D

G

A

A

C

L

L

A

G

A

A

S

G

V

M

F

F

H

H

K

R

Q

G

I

E

I

F

N

T

I

V

G

N

E

D

L

V

K

K

A

E

Y

Y

L

L

A

L

T

E

Q

H

G

G

V

L

E

K

I

V

R

R

I

M

R239 (= R5) mutation R->A,H,K: 1000-fold decrease in catalytic efficiency. Uncoupling of glutaminase activity from the cyclase activity.
K258 (= K19) mutation to A: No activity. Uncoupling of glutaminase activity from the cyclase activity.; mutation to R: Small reduction of activity.
K360 (= K99) mutation K->A,R: Almost no effect on activity.

1ox4B Towards understanding the mechanism of the complex cyclization reaction catalyzed by imidazole glycerophosphate synthase (see paper)
32% identity, 99% coverage: 2:257/258 of query aligns to 239:537/538 of 1ox4B

query
sites
1ox4B

L

L

A

T

K

R

R

R

I

I

P

A

C

C

L

L

D
|
D

V

V

R

R

-

T

-

N

-

D

-

Q

-

G

D

D

G

L

Q

V

V

V

V

T

K

K

G

G

V

-

Q

-

F

-

R

-

N

-

H

-

E

-

I

D

I

L

G

G

D

K

I

P

V

V

P

Q

L

L

A

A

K

Q

R

K

Y

Y

A

Y

E

Q

G

G

A

A

D

D

E

E

L

V

V

T

F

F

Y

L

D

N

I

I

T

T

A

S

S

F

S

R

D

D

G

C

R

P

V

L

V

K

D

D

K

T

S

P

W

M

V

L

-

E

-

V

-

L

S

K

R

Q

V

A

A

A

E

K

V

T

I

V

D

F

I

V

P

P

F

L

C

T

V

V

A

G

G

G

I

I

K

K

-

D

-

I

-

V

-

D

-

V

-

D

-

G

-

T

-

K

-

I

-

P

S

A

L

L

E

E

D

V

A

A

A

S

K

L

I

Y

L

F

S

R

F

S

G

G

A

A

D

D

K

K

I

V

S

S

I

I

N

G

S

T

P

D

A

A

L

V

A

Y

D

A

P

A

T

E

L

K

I

Y

T

Y

R

E

L

L

A

G

D

N

R

R

-

G

-

D

-

G

-

T

-

S

-

P

-

I

-

E

-

T

-

I

-

S

-

K

-

A

F

Y

G

G

V

A

Q

Q

C

A

I

V

V

V

V

I

G

S

I

V

D
|
D

-

P

-

K

-

R

-

V

-

Y

-

V

-

N

-

S

-

Q

-

A

-

D

-

T

-

K

-

N

-

K

-

V

-

F

-

E

T

T

W

E

Y

Y

D

P

A

G

E

P

T

N

G

G

K

E

-

K

-

Y

-

C

-

W

Y

Y

H

Q

V

C

N

T

Q

I

Y

K

T

G

G

G

D

R

E

E

S

S

R

R

T

D

R

-

V

L

T

G

Q

V

W

W

E

E

T

-

L

-

D

-

W

L

V

T

Q

R

E

A

V

C

Q

E

K

A

R

L

G

G

A

A

G

G

E

E

I

I

V

L

L

L

N

N

M

C

M

I

N

D

Q

K

D

D

G
|
G

V

S

R

N

N

S

G

G

Y

Y

D

D

L

L

E

E

Q

L

L

I

K

E

K

H

V

V

R

K

E

D

V

A

C

V

H

K

V

I

P

P

L

V

I

I

A

A

S

S

G
x
S

G

G

A

A

G

G

T

V

M

P

E

E

H

H

F

F

L

E

E

E

A

A

F

F

R

L

D

K

A

T

D

R

V

A

D

D

G

A

A

C

L

L

A

G

A
|
A

S
x
G

V

M

F

F

H

H

K

R

Q

G

I

E

I

F

N

T

I

V

G

N

E

D

L

V

K

K

A

E

Y

Y

L

L

A

L

T

E

Q

H

G

G

V

L

E

K

I

V

R

R

I

M

active site: D248 (= D11), D392 (= D130)
binding pyrophosphate 2-: G463 (= G183), S488 (≠ G208), A511 (= A231), G512 (≠ S232)

1ox5A Towards understanding the mechanism of the complex cyclization reaction catalyzed by imidazole glycerophosphate synthase (see paper)
31% identity, 99% coverage: 2:257/258 of query aligns to 239:531/532 of 1ox5A

query
sites
1ox5A

L

L

A

T

K

R

R

R

I

I

P

A

C

C

L

L

D
|
D

V

V

R

R

D

T

G

N

Q

D

V

Q

V

G

K

D

G

L

V

V

Q

V

F

T

R

K

N

-

H

-

E

-

I

-

I

L

G

G

D

K

I

P

V

V

P

Q

L

L

A

A

K

Q

R

K

Y

Y

A

Y

E

Q

G

G

A

A

D

D

E

E

L

V

V

T

F

F

Y
x
L

D

N

I
|
I

T

T

A

C

S

P

-

L

S

K

D

D

G

T

R

P

V

M

V

L

D

E

K

-

S

-

W

V

V

L

S

K

R

Q

V

A

A

A

E

K

V

T

I

V

D

F

I

V

P

P

F

L

C

T

V

V

A

G

G
|
G

G

G

I

I

K

K

-

D

-

I

-

V

-

D

-

V

-

D

-

G

-

T

-

K

-

I

-

P

S

A

L

L

E

E

D

V

A

A

A

S

K

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Y

L

F

S

R

F

S

G

G

A

A

D

D

K

K

I

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S

S

I

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x
G

S
x
T

P

D

A

A

L

V

A

Y

D

A

P

A

T

E

L

K

I

Y

T

Y

R

E

L

L

A

G

D

N

R

R

-

G

-

D

-

G

-

T

-

S

-

P

-

I

-

E

-

T

-

I

-

S

-

K

-

A

F

Y

G

G

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A

Q

Q

C

A

I

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x
S

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|
D

-

P

-

K

-

R

-

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-

Y

-

V

-

N

-

S

-

Q

-

A

-

D

-

T

-

K

-

N

-

K

-

V

-

F

-

E

T

T

W

E

Y

Y

D

P

A

G

E

P

T

N

G

G

K

E

-

K

-

Y

-

C

-

W

Y

Y

H

Q

V

C

N

T

Q

I

Y

K

T
x
G

G

G

D

R

E

E

S

S

R

R

T

D

R

-

V

L

T

G

Q

V

W

W

E

E

T

-

L

-

D

-

W

L

V

T

Q

R

E

A

V

C

Q

E

K

A

R

L

G

G

A

A

G

G

E

E

I

I

V

L

L

L

N
|
N

M

C

M

I

N

D

Q

K

D
|
D

G
|
G

V

S

R

N

N

S

G

G

Y

Y

D

D

L

L

E

E

Q

L

L

I

K

E

K

H

V

V

R

K

E

D

V

A

C

V

H

K

V

I

P

P

L

V

I

I

A

A

S

S

G
x
S

G

G

A

A

G

G

T

V

M

P

E

E

H

H

F

F

L

E

E

E

A

A

F

F

R

L

D

K

A

T

D

R

V

A

D

D

G

A

A

C

L

L

A

G

A
|
A

S
x
G

V

M

F

F

H

H

K

R

Q

G

I

E

I

F

N

T

I

V

G

N

E

D

L

V

K

K

A

E

Y

Y

L

L

A

L

T

E

Q

H

G

G

V

L

E

K

I

V

R

R

I

M

active site: D248 (= D11), D386 (= D130)
binding phosphoric acid mono-[5-({[5-carbamoyl-3-(5-phosphonooxy-5-deoxy-ribofuranosyl)- 3h-imidazol-4-ylamino]-methyl}-amino)-2,3,4-trihydroxy-pentyl] ester: L283 (≠ Y50), I285 (= I52), G313 (= G81), G346 (≠ N103), T347 (≠ S104), S384 (≠ G128), D386 (= D130), G424 (≠ T146), N451 (= N177), D456 (= D182), G457 (= G183), S482 (≠ G208), A505 (= A231), G506 (≠ S232)

3tdmA Computationally designed tim-barrel protein, halfflr (see paper)
45% identity, 46% coverage: 123:240/258 of query aligns to 1:113/120 of 3tdmA

query
sites
3tdmA

Q

Q

C

A

I

V

V

V

G

A

I

I

D

D

T

A

W

-

Y

-

D

K

A

R

E

V

T

D

G

G

K

E

Y

F

H

M

V

V

N

F

Q

T

Y

Y

T

S

G

G

D

K

E

K

S

N

R

-

T

-

R

-

V

-

T

T

Q

G

W

I

E

L

T

L

L

R

D

D

W

W

V

V

Q

V

E

E

V

V

Q

E

K

K

R

R

G

G

A

A

G

G

E

E

I

I

V

L

L

L

N

T

M

S

M

I

N

D

Q

R

D
|
D

G
|
G

V

T

R

K

N

S

G

G

Y

Y

D

D

L

T

E

E

Q

M

L

I

K

R

K

F

V

V

R

R

E

P

V

L

C

T

H

T

V

L

P

P

L

I

I

I

A

A

S

S

G

G

A

A

G

G

T

K

M

M

E

E

H

H

F

F

L

L

E

E

A

A

F

F

-

L

R

R

D

G

A

A

D

D

V

K

D

V

G

S

A

I

L
x
N

A
x
T

A

A

S

A

V

V

F

E

H

N

K

P

Q

S

I

L

I

I

N

T

binding phosphate ion: D54 (= D182), G55 (= G183), N102 (≠ L229), T103 (≠ A230)

5dn1A Crystal structure of phosphoribosyl isomerase a from streptomyces coelicolor (see paper)
26% identity, 92% coverage: 6:243/258 of query aligns to 6:234/240 of 5dn1A

query
sites
5dn1A

I

L

P

P

C

A

L

V

D
|
D

V

V

R

R

D

D

G

G

Q

Q

V

A

V

V

K

R

G

L

V

V

Q

H

F
x
G

R

E

N

S

H

G

E

T

I

E

I

T

G

S

D

Y

I

G

V

S

P

P

L

L

-

E

-

A

A

A

K

L

R

A

Y

W

A

Q

E

R

E

S

G

G

A

A

D

E

E

W

L

L

V

H

F

L

Y

V

D

D

I
x
L

T

D

A

A

S

A

S
x
F

D

-

G

G

R

T

V

G

V

D

D

N

K

R

S

A

W

L

V

I

S

A

R

E

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V

A

A

E

Q

V

A

I

M

D

D

I

I

P

K

F

V

C

E

V

L

A
x
S

G

G

G
|
G

I

I

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x
R

S

D

L

D

E

D

D

T

A

L

A

A

K

A

I

A

L

L

S

A

F

T

G

G

A

C

D

T

K

R

I

V

S

N

I

L

N
x
G

S
x
T

P

A

A

A

L

L

A

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D

T

P

P

T

E

L

W

I

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T

A

R

K

L

V

A

I

D

A

R

E

F

H

G

G

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D

Q

K

C

-

I

I

V

A

V

V

G

G

I

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|
D

T

V

W

R

Y

G

D

T

A

T

E

L

T

R

G

G

K

R

Y
x
G

H
x
W

V

-

N

-

Q

-

Y

-

T

-

G

-

D

-

E

-

S

T

R

R

T

D

R

G

V

G

T

D

Q

L

W

Y

E

E

T

T

L

L

D

D

W

-

V

-

Q

-

E

R

V

L

Q

N

K

K

R

E

G

G

A

C

G

A

E

R

I

Y

V

V

L

V

N

T

M

D

M

I

N

A

Q

K

D

D

G

G

V

T

R

L

N

Q

G

G

Y

P

D

N

L

L

E

E

Q

L

L

L

K

K

K

N

V

V

R

C

E

A

V

A

C

T

H

D

V

R

P

P

L

V

I

V

A

A

S

S

G

G

A

V

G

S

T

S

M

L

E

D

H

D

F

L

-

R

-

A

L

I

E

A

A

G

F

L

R

V

D

P

A

A

D

G

V

V

D

E

G

G

A

A

L

I

A

V

A

G

S

K

V

A

F

L

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Y

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A

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K

I

A

I

F

N

T

I

L

G

E

E

E

active site: D11 (= D11), D130 (= D130)
binding aminoimidazole 4-carboxamide ribonucleotide: G23 (≠ F23), L54 (≠ I52), F58 (≠ S56), S81 (≠ A80), G83 (= G82), R85 (≠ K84), G104 (≠ N103), T105 (≠ S104), G140 (≠ Y140), W141 (≠ H141)

P16250 Phosphoribosyl isomerase A; 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; N-(5'-phosphoribosyl)anthranilate isomerase; PRAI; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; EC 5.3.1.16; EC 5.3.1.24 from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see paper)
26% identity, 92% coverage: 6:243/258 of query aligns to 6:234/240 of P16250

query
sites
P16250

I

L

P

P

C

A

L

V

D
|
D

V

V

R

R

D

D

G

G

Q

Q

V

A

V

V

K
x
R

G

L

V

V

Q

H

F

G

R

E

N

S

H

G

E

T

I

E

I

T

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D

Y

I

G

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S

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P

L

L

-

E

-

A

A

A

K

L

R

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W

A

Q

E

R

E

S

G

G

A

A

D

E

E

W

L

L

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H

F

L

Y

V

D

D

I

L

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D

A

A

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A

S

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V

G

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D

N

K

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A

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V

A

A

E

Q

V

A

I

M

D

D

I

I

P

K

F

V

C

E

V

L

A
x
S

G

G

I

I

K

R

S

D

L

D

E

D

D

T

A

L

A

A

K

A

I

A

L

L

S

A

F

T

G

G

A

C

D

T

K

R

I

V

S

N

I

L

N

G

S

T

P

A

A

A

L

L

A

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D

T

P

P

T

E

L

W

I

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T

A

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K

L

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A

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A

R

E

F

H

G

G

V

D

Q

K

C

-

I

I

V

A

V

V

G

G

I

L

D
|
D

T

V

W

R

Y

G

D

T

A

T

E

L

T

R

G

G

K

R

Y

G

H

W

V

-

N

-

Q

-

Y

-

T

-

G

-

D

-

E

-

S

T

R

R

T

D

R

G

V

G

T

D

Q

L

W

Y

E

E

T

T

L

L

D

D

W

-

V

-

Q

-

E

R

V

L

Q

N

K

K

R

E

G

G

A

C

G

A

E

R

I

Y

V

V

L

V

N
x
T

M

D

M

I

N

A

Q

K

D
|
D

G

G

V

T

R

L

N

Q

G

G

Y

P

D

N

L

L

E

E

Q

L

L

L

K

K

K

N

V

V

R

C

E

A

V

A

C

T

H

D

V

R

P

P

L

V

I

V

A

A

S

S

G

G

A

V

G

S

T

S

M

L

E

D

H

D

F

L

-

R

-

A

L

I

E

A

A

G

F

L

R

V

D

P

A

A

D

G

V

V

D

E

G

G

A

A

L

I

A

V

A

G

S

K

V

A

F

L

H

Y

K

A

Q

K

I

A

I

F

N

T

I

L

G

E

E

E

D11 (= D11) mutation to A: No activity.
R19 (≠ K19) mutation to A: No effect on activity toward PRA. No activity toward ProFAR.
S81 (≠ A80) mutation to T: No activity toward PRA. Almost no effect on activity toward ProFAR.
D130 (= D130) mutation to A: Very low activity toward PRA. No activity toward ProFAR.; mutation to Q: No activity.
T166 (≠ N177) mutation to A: No activity.
D171 (= D182) mutation to A: Low activity toward PRA. No activity toward ProFAR.

3zs4A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase with bound prfar
24% identity, 90% coverage: 6:237/258 of query aligns to 5:231/244 of 3zs4A

query
sites
3zs4A

I

L

P

P

C
x
A

L

V

D
|
D

V

V

R

V

D

E

G

G

Q

R

V

A

V

V

K
x
R

G

L

V

V

Q

Q

F

G

R

K

-

A

-

G

N

S

H

Q

E

T

I

E

I

Y

G

G

D

S

I

A

V

V

P

D

L

A

A

A

K

L

R

G

Y

W

A

Q

E

R

E

D

G

G

A

A

D

E

E

W

L

I

V

H

F

L

Y

V

D

D

I

L

T

D

A

A

S
x
A

S
x
F

D

-

G

G

R

R

V

G

V

S

D

N

K

H

S

E

W

L

V

L

S

A

R

E

V

V

A

V

E

G

V

K

I

L

D

D

I

V

P

Q

F

V

C

E

V

L

A
x
S

G

G

G
|
G

I

I

K
x
R

S

D

L

D

E

E

D

S

A

L

A

A

K

A

I

A

L

L

S

A

F

T

G

G

A

C

D

A

K

R

I

V

S

N

I

V

N
x
G

S
x
T

P

A

A

A

L

L

A

E

D

N

P

P

T

Q

L

W

I

C

T

A

R

R

L

V

A

I

D

G

R

E

F

H

G

G

V

D

Q

Q

C

-

I

V

V

A

V

V

G

G

I

L

D
|
D

T

V

W

-

Y

-

D

-

A

-

E

-

T

-

G

-

K

-

Y

-

H

-

V

-

N

-

Q

Q

Y

I

T

I

G

D

D

G

E

E

S

H

R

R

T

L

R

R

V

G

T

R

Q
x
G

W
|
W

E

E

T

T

-

D

-

G

-

D

-

L

L

W

D

D

W

V

V

L

Q

E

E

R

V

L

Q

D

K

S

R

E

G

G

A

C

G

S

E

R

I

F

V

V

L

V

N

T

M

D

M

I

N

T

Q

K

D
|
D

G
|
G

V

T

R

L

N

G

G

G

Y

P

D

N

L

L

E

D

Q

L

L

L

K

A

K

G

V

V

R

A

E

D

V

R

C

T

H

D

V

A

P

P

L

V

I

I

A

A

S

S

G
|
G

A

V

G

S

T

S

M

L

E

D

H

D

F

L

-

R

-

A

L

I

E

A

A

T

F

L

R

T

D

H

A

R

D

G

V

V

D

E

G

G

A

A

L
x
I

A

V

A
x
G

S
x
K

V

A

F

L

H

Y

K

A

Q

R

active site: D10 (= D11), D129 (= D130)
binding phosphoric acid mono-[5-({[5-carbamoyl-3-(5-phosphonooxy-5-deoxy-ribofuranosyl)- 3h-imidazol-4-ylamino]-methyl}-amino)-2,3,4-trihydroxy-pentyl] ester: A8 (≠ C9), D10 (= D11), R18 (≠ K19), A56 (≠ S55), F57 (≠ S56), S80 (≠ A80), G82 (= G82), R84 (≠ K84), G103 (≠ N103), T104 (≠ S104), D129 (= D130), G143 (≠ Q156), W144 (= W157), D174 (= D182), G175 (= G183), G200 (= G208), G201 (= G209), I223 (≠ L229), G225 (≠ A231), K226 (≠ S232)

5abtA S.Enterica hisa mutant d7n, g102a, v106m, d176a
30% identity, 50% coverage: 6:134/258 of query aligns to 2:133/246 of 5abtA

query
sites
5abtA

I

I

P

P

C

A

L

L

D
x
N

V

L

R

I

D

D

G

G

Q

T

V

V

K

R

-

L

-

H

-

Q

G
|
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D

active site: N7 (≠ D11), D129 (= D130)
binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: N7 (≠ D11), G19 (= G20), V49 (≠ Y50), L51 (≠ I52), G81 (= G82), R83 (≠ K84), V100 (≠ S101), A102 (≠ N103), S103 (= S104), D129 (= D130)

Sites not aligning to the query:

binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: 144, 145, 177, 202, 203, 223, 225, 226

Query Sequence

>16133 b2025 imidazole glycerol phosphate synthase subunit HisF (NCBI)
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRV
VDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISINSPALADPTLITRLADRF
GVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETLDWVQEVQKRGAGEIVLNMMN
QDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
IGELKAYLATQGVEIRIC

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory