SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 16149 b2041 dTDP-glucose 4,6 dehydratase, NAD(P)-binding (NCBI) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1kewA The crystal structure of dtdp-d-glucose 4,6-dehydratase (rmlb) from salmonella enterica serovar typhimurium with thymidine diphosphate bound (see paper)
88% identity, 100% coverage: 1:361/361 of query aligns to 1:361/361 of 1kewA

query
sites
1kewA

M

M

K

K

I

I

L

L

V

I

T

T

G
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G

G

G

A
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A

G
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G

F
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F

I
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I

G

G

S

S

A

A

V

V

R

R

H

H

I

I

N

K

N

N

T

T

Q

Q

D

D

S

T

V

V

N

N

V

I

D
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D

K
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K

L
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L

T
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T

Y

Y

A
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A

G
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G

N

N

R

L

E

E

S

S

L

L

A

S

D

D

V

I

S

S

D

E

S

S

E

N

R

R

Y

Y

V

N

F

F

E

E

H

H

A
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A

D
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D

I
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I

C

C

D

D

A

S

P

A

A

E

M

I

A

T

R

R

I

I

F

F

A

E

Q

Q

H

Y

Q

Q

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P

D

D

A

A

V

V

M

M

H

H

L
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L

A
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A

E

E

S
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S

H
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H

V
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V

D

D

R

R

S

S

I

I

T

T

G

G

P

P

A

A

F

F

I

I

E

E

T
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T

N

N

I

I

V

V

G

G

T

T

Y

Y

V

A

L

L

E

E

A

V

A

A

R

R

N

K

Y

Y

W

W

S

S

A

A

L

L

D

G

S

E

D

D

K

K

N

N

S

N

F

F

R

R

F

F

H

H

I
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I

S
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S

T
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T

D
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D

E
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E

V

V

Y

Y

G

G

D

D

L

L

P

P

H

H

P

P

D

D

E

E

V

V

N

E

N

N

T

S

E

V

E

T

L
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L

P

P

L
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L

F
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F

T

T

E

E

T
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T

T

T

A

A

Y

Y

A

A

P

P

S

S

P

P

Y
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Y

S

S

A

A

S

S

K
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K

A

A

S

S

D

D

H

H

L

L

V

V

R

R

A

A

W

W

K

R

R

R

T

T

Y

Y

G

G

L

L

P

P

T

T

I

I

V

V

T

T

N

N

C
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C

S
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S

N
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N

Y

Y

G

G

P

P

Y

Y

H

H

F

F

P

P

E

E

K
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K

L
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L

I

I

P

P

L

L

V

V

I

I

L

L

N

N

A

A

L

L

E

E

G

G

K

K

A

P

L

L

P
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P

I
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I

Y
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Y

G

G

K

K

G

G

D

D

Q

Q

I

I

R
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R

D

D

W

W

L

L

Y

Y

V

V

E

E

D

D

H

H

A

A

R

R

A

A

L

L

Y

H

T

M

V

V

T

T

E

E

G

G

K

K

A

A

G

G

E

E

T

T

Y

Y

N

N

I

I

G

G

H

H

N

N

E

E

K

K

N
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N

I

L

D

D

V

V

L

F

T

T

I

I

C

C

D

D

L

L

D

D

E

E

I

I

V

V

P

P

K

K

E

A

K

T

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S

Y

Y

R

R

E

E

Q

Q

I

I

T

T

Y

Y

V

V

A

A

D

D

R
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R

P

P

G

G

H
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H

D

D

R

R

Y

Y

A

A

I

I

D

D

A

A

E

G

K

K

I

I

G

S

R

R

A

E

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L

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G

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W

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K

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F

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G

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I

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R

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K

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V

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E

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W

Y

Y

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L

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A

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T

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W

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D

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N

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V

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K

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S

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G

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A

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Y

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S

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W

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I

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Y

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E

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G

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R

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Q

active site: T133 (= T133), D134 (= D134), E135 (= E135), L152 (= L152), L154 (= L154), F155 (= F155), T158 (= T158), Y167 (= Y167), K171 (= K171)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), A9 (= A9), G10 (= G10), F11 (= F11), I12 (= I12), D32 (= D32), K33 (= K33), L34 (= L34), T35 (= T35), A37 (= A37), G38 (= G38), A57 (= A57), D58 (= D58), I59 (= I59), L80 (= L80), A81 (= A81), A82 (= A82), S84 (= S84), T99 (= T99), I131 (= I131), S132 (= S132), T133 (= T133), Y167 (= Y167), K171 (= K171), C194 (= C194), S195 (= S195), N196 (= N196), N197 (= N197)
binding thymidine-5'-diphosphate: H85 (= H85), V86 (= V86), E135 (= E135), N196 (= N196), K206 (= K206), L207 (= L207), P222 (= P222), I223 (= I223), Y224 (= Y224), R231 (= R231), N266 (= N266), R297 (= R297), H300 (= H300), Y357 (= Y357)

1keuA The crystal structure of dtdp-d-glucose 4,6-dehydratase (rmlb) from salmonella enterica serovar typhimurium with dtdp-d-glucose bound (see paper)
88% identity, 100% coverage: 1:361/361 of query aligns to 1:361/361 of 1keuA

query
sites
1keuA

M

M

K

K

I

I

L

L

V

I

T

T

G
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G

G

G

A
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A

G
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G

F
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F

I
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I

G

G

S

S

A

A

V

V

R

R

H

H

I

I

N

K

N

N

T

T

Q

Q

D

D

S

T

V

V

N

N

V

I

D
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D

K
|
K

L
|
L

T
|
T

Y

Y

A
|
A

G
|
G

N

N

R

L

E

E

S

S

L

L

A

S

D

D

V

I

S

S

D

E

S

S

E

N

R

R

Y

Y

V

N

F

F

E

E

H

H

A
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A

D
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D

I
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I

C

C

D

D

A

S

P

A

A

E

M

I

A

T

R

R

I

I

F

F

A

E

Q

Q

H

Y

Q

Q

P

P

D

D

A

A

V

V

M

M

H

H

L
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L

A
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A

E

E

S
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S

H
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H

V
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V

D

D

R

R

S

S

I

I

T

T

G

G

P

P

A

A

F

F

I

I

E

E

T
|
T

N

N

I

I

V

V

G

G

T

T

Y

Y

V

A

L

L

E

E

A

V

A

A

R

R

N

K

Y

Y

W

W

S

S

A

A

L

L

D

G

S

E

D

D

K

K

N

N

S

N

F

F

R

R

F

F

H

H

I
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I

S
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S

T
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T

D
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D

E
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E

V

V

Y

Y

G

G

D

D

L

L

P

P

H

H

P

P

D

D

E

E

V

V

N

E

N

N

T

S

E

V

E

T

L
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L

P

P

L
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L

F
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F

T

T

E

E

T
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T

T

T

A

A

Y

Y

A

A

P

P

S

S

P

P

Y
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Y

S

S

A

A

S

S

K
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K

A

A

S

S

D

D

H

H

L

L

V

V

R

R

A

A

W

W

K

R

R

R

T

T

Y

Y

G

G

L

L

P

P

T

T

I

I

V

V

T

T

N

N

C
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C

S
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S

N
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N

Y

Y

G

G

P

P

Y

Y

H

H

F

F

P

P

E

E

K
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K

L
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L

I

I

P

P

L

L

V

V

I

I

L

L

N

N

A

A

L

L

E

E

G

G

K

K

A

P

L

L

P
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P

I
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I

Y
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Y

G

G

K

K

G

G

D

D

Q

Q

I

I

R
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R

D

D

W

W

L

L

Y

Y

V

V

E

E

D

D

H

H

A

A

R

R

A

A

L

L

Y

H

T

M

V

V

T

T

E

E

G

G

K

K

A

A

G

G

E

E

T

T

Y

Y

N

N

I

I

G

G

H

H

N

N

E

E

K

K

N
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N

I

L

D

D

V

V

L

F

T

T

I

I

C

C

D

D

L

L

D

D

E

E

I

I

V

V

P

P

K

K

E

A

K

T

S

S

Y

Y

R

R

E

E

Q

Q

I

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T

T

Y

Y

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V

A

A

D

D

R
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R

P

P

G

G

H
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H

D

D

R

R

Y
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Y

A

A

I

I

D

D

A

A

E

G

K

K

I

I

G

S

R

R

A

E

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L

G

G

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W

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K

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P

Q

L

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G

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I

R

R

K

K

T

T

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V

E

E

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W

Y

Y

L

L

S

A

N

N

T

T

K

Q

W

W

V

V

D

N

N

N

V

V

K

K

S

S

G

G

A

A

Y

Y

Q

Q

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S

W

W

I

I

E

E

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N

N

Y
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Y

E

E

G

G

R

R

Q

Q

active site: T133 (= T133), D134 (= D134), E135 (= E135), L152 (= L152), L154 (= L154), F155 (= F155), T158 (= T158), Y167 (= Y167), K171 (= K171)
binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: S84 (= S84), H85 (= H85), V86 (= V86), T133 (= T133), D134 (= D134), E135 (= E135), Y167 (= Y167), S195 (= S195), N196 (= N196), K206 (= K206), L207 (= L207), P222 (= P222), I223 (= I223), Y224 (= Y224), R231 (= R231), N266 (= N266), R297 (= R297), H300 (= H300), Y304 (= Y304), Y357 (= Y357)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), A9 (= A9), G10 (= G10), F11 (= F11), I12 (= I12), D32 (= D32), K33 (= K33), L34 (= L34), T35 (= T35), A37 (= A37), G38 (= G38), A57 (= A57), D58 (= D58), I59 (= I59), L80 (= L80), A81 (= A81), A82 (= A82), S84 (= S84), T99 (= T99), I131 (= I131), S132 (= S132), T133 (= T133), Y167 (= Y167), K171 (= K171), C194 (= C194), S195 (= S195), N196 (= N196), N197 (= N197)

P26391 dTDP-glucose 4,6-dehydratase; EC 4.2.1.46 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
88% identity, 100% coverage: 1:361/361 of query aligns to 1:361/361 of P26391

query
sites
P26391

M

M

K

K

I

I

L

L

V

I

T

T

G

G

A

A

G

G

F
|
F

I
|
I

G

G

S

S

A

A

V

V

R

R

H

H

I

I

N

K

N

N

T

T

Q

Q

D

D

S

T

V

V

N

N

V

I

D
|
D

K
|
K

L
|
L

T
|
T

Y

Y

A

A

G

G

N

N

R

L

E

E

S

S

L

L

A

S

D

D

V

I

S

S

D

E

S

S

E

N

R

R

Y

Y

V

N

F

F

E

E

H

H

A

A

D
|
D

I
|
I

C

C

D

D

A

S

P

A

A

E

M

I

A

T

R

R

I

I

F

F

A

E

Q

Q

H

Y

Q

Q

P

P

D

D

A

A

V

V

M

M

H

H

L
|
L

A
|
A

E
|
E

S
|
S

H

H

V

V

D

D

R

R

S

S

I

I

T

T

G

G

P

P

A

A

F

F

I

I

E

E

T
|
T

N

N

I

I

V

V

G

G

T

T

Y

Y

V

A

L

L

E

E

A

V

A

A

R

R

N

K

Y

Y

W

W

S

S

A

A

L

L

D

G

S

E

D

D

K

K

N

N

S

N

F

F

R

R

F

F

H

H

I

I

S

S

T
|
T

D

D

E

E

V

V

Y

Y

G

G

D

D

L

L

P

P

H

H

P

P

D

D

E

E

V

V

N

E

N

N

T

S

E

V

E

T

L

L

P

P

L

L

F

F

T

T

E

E

T

T

A

A

Y

Y

A

A

P

P

S

S

P

P

Y
|
Y

S
|
S

A
|
A

S
|
S

K
|
K

A

A

S

S

D

D

H

H

L

L

V

V

R

R

A

A

W

W

K

R

R

R

T

T

Y

Y

G

G

L

L

P

P

T

T

I

I

V

V

T

T

N

N

C

C

S

S

N
|
N

Y

Y

G

G

P

P

Y

Y

H

H

F

F

P

P

E

E

K
|
K

L
|
L

I

I

P

P

L

L

V

V

I

I

L

L

N

N

A

A

L

L

E

E

G

G

K

K

A

P

L

L

P
|
P

I
|
I

Y
|
Y

G

G

K

K

G

G

D

D

Q

Q

I

I

R
|
R

D

D

W

W

L

L

Y

Y

V

V

E

E

D

D

H

H

A

A

R

R

A

A

L

L

Y

H

T

M

V

V

T

T

E

E

G

G

K

K

A

A

G

G

E

E

T

T

Y

Y

N

N

I

I

G

G

H

H

N

N

E

E

K

K

N
|
N

I

L

D

D

V

V

L

F

T

T

I

I

C

C

D

D

L

L

D

D

E

E

I

I

V

V

P

P

K

K

E

A

K

T

S

S

Y

Y

R

R

E

E

Q

Q

I

I

T

T

Y

Y

V

V

A

A

D
|
D

R
|
R

P
|
P

G
|
G

H
|
H

D

D

R

R

Y

Y

A

A

I

I

D

D

A

A

E

G

K

K

I

I

G

S

R

R

A

E

L

L

G

G

W

W

K

K

P

P

Q

L

E

E

T

T

F

F

E

E

S

S

G

G

I

I

R

R

K

K

T

T

V

V

E

E

W

W

Y

Y

L

L

S

A

N

N

T

T

K

Q

W

W

V

V

D

N

N

N

V

V

K

K

S

S

G

G

A

A

Y

Y

Q

Q

S

S

W

W

I

I

E

E

Q

Q

N

N

Y
|
Y

E

E

G

G

R

R

Q

Q

FI 11:12 (= FI 11:12) binding
DKLT 32:35 (= DKLT 32:35) binding
DI 58:59 (= DI 58:59) binding
LAAES 80:84 (= LAAES 80:84) binding
S84 (= S84) binding
T99 (= T99) binding
T133 (= T133) binding
YSASK 167:171 (= YSASK 167:171) binding
N196 (= N196) binding
N197 (= N197) binding
KL 206:207 (= KL 206:207) binding
PIY 222:224 (= PIY 222:224) binding
R231 (= R231) binding
N266 (= N266) binding
DRPGH 296:300 (= DRPGH 296:300) binding
Y357 (= Y357) binding

P27830 dTDP-glucose 4,6-dehydratase 2; EC 4.2.1.46 from Escherichia coli (strain K12) (see 2 papers)
74% identity, 97% coverage: 2:350/361 of query aligns to 3:347/355 of P27830

query
sites
P27830

K

K

I

I

L

L

V

I

T

T

G

G

A

A

G

G

F
|
F

I
|
I

G

G

S

S

A

A

V

L

V

V

R

R

H

Y

I

I

N

N

N

E

T

T

Q

S

D

D

S

A

V

V

N

V

V

V

D
|
D

K
|
K

L
|
L

T
|
T

Y

Y

A

A

G

G

N

N

R

L

E

M

S

S

L

L

A

A

D

P

V

V

S

A

D

Q

S

S

E

E

R

R

Y

F

V

A

F

F

E

E

H

K

A

V

D
|
D

I
|
I

C

C

D

D

A

R

P

A

A

E

M

L

A

A

R

R

I

V

F

F

A

T

Q

E

H

H

Q

Q

P

P

D

D

A

C

V

V

M

M

H

H

L

L

A

A

E

E

S

S

H

H

V

V

D

D

R

R

S

S

I

I

T

D

G

G

P

P

A

A

F

F

I

I

E

E

T
|
T

N

N

I

I

V

V

G

G

T

T

Y

Y

V

T

L

L

E

E

A

A

R

R

N

A

Y

Y

W

W

S

N

A

A

L

L

D

T

S

E

D

D

K

K

N

S

S

A

F

F

R

R

F

F

H

H

I

I

S

S

T

T

D
|
D

E
|
E

V

V

Y

Y

G

G

D

D

L

L

P

H

H

S

P

T

D

D

E

D

V

-

N

-

T

-

E

-

L

-

P

-

L

F

F

F

T

T

E

E

T

T

A

P

Y

Y

A

A

P

P

S

S

P

P

Y
|
Y

S
|
S

A
|
A

S
|
S

K
|
K

A

A

S

S

D

D

H

H

L

L

V

V

R

R

A

A

W

W

K

L

R

R

T

T

Y

Y

G

G

L

L

P

P

T

T

I

L

V

I

T

T

N

N

C

C

S

S

N

N

N
|
N

Y

Y

G

G

P

P

Y

Y

H

H

F

F

P

P

E

E

K

K

L

L

I

I

P

P

L

L

V

M

I

I

L

L

N

N

A

A

L

L

E

A

G

G

K

K

A

S

L

L

P

P

I

V

Y

Y

G

G

K

N

G

G

D

Q

Q

Q

I

I

R

R

D

D

W

W

L

L

Y

Y

V

V

E

E

D

D

H

H

A

A

R

R

A

A

L

L

Y

Y

T

C

V

V

V

A

T

T

E

T

G

G

K

K

A

V

G

G

E

E

T

T

Y

Y

N

N

I

I

G

G

H

H

N

N

E

E

K

R

K

K

N

N

I

L

D

D

V

V

L

E

T

T

I

I

C

C

D

E

L

L

D

E

E

E

I

L

V

A

P

P

K

N

E

K

-

P

-

H

-

G

-

V

K

A

S

H

Y

Y

R

R

E

D

Q

L

I

I

T

T

Y

F

V

V

A

A

D

D

R

R

P

P

G

G

H

H

D

D

R

L

R

R

Y

Y

A

A

I

I

D

D

A

A

E

S

K

K

I

I

G

A

R

R

A

E

L

L

G

G

W

W

K

L

P

P

Q

Q

E

E

T

T

F

F

E

E

S

S

G

G

I

M

R

R

K

K

T

T

V

V

E

Q

W

W

Y

Y

L

L

S

A

N

N

T

E

K

S

W

W

V

W

D

K

N

Q

V

V

K

Q

S

D

G

G

A

S

Y

Y

Q

Q

FI 12:13 (= FI 11:12) binding
DKLT 33:36 (= DKLT 32:35) binding
DI 59:60 (= DI 58:59) binding
T100 (= T99) binding
D135 (= D134) active site, Proton donor
E136 (= E135) active site, Proton acceptor
Y160 (= Y167) active site, Proton acceptor
YSASK 160:164 (= YSASK 167:171) binding
N190 (= N197) binding

1bxkB Dtdp-glucose 4,6-dehydratase from e. Coli
72% identity, 97% coverage: 2:350/361 of query aligns to 3:341/344 of 1bxkB

query
sites
1bxkB

K

K

I

I

L

L

V

I

T

T

G
|
G

G

G

A
|
A

G
|
G

F
|
F

I
|
I

G

G

S

S

A

A

V

L

V

V

R

R

H

Y

I

I

N

N

N

E

T

T

Q

S

D

D

S

A

V

V

N

V

V

V

D
|
D

K
|
K

L
|
L

T
|
T

Y

Y

A
|
A

G
|
G

N

N

R

L

E

M

S

S

L

L

A

A

D

P

V

V

S

A

D

Q

S

S

E

E

R

R

Y

F

V

A

F

F

E

E

H

K

A
x
V

D
|
D

I
|
I

C

C

D

D

A

R

P

A

A

E

M

L

A

A

R

R

I

V

F

F

A

T

Q

E

H

H

Q

Q

P

P

D

D

A

C

V

V

M

M

H

H

L
|
L

A
|
A

E

E

S

S

H

H

V

V

D

D

R

R

S

S

I

I

T

D

G

G

P

P

A

A

F

F

I

I

E

E

T
|
T

N

N

I

I

V

V

G

G

T

T

Y

Y

V

T

L

L

E

E

A

A

R

R

N

A

Y

Y

W

W

S

N

A

A

L

L

D

T

S

E

D

D

K

K

N
x
S

S

A

F

F

R

R

F

F

H

H

I
|
I

S
|
S

T
|
T

D
|
D

E
|
E

V

V

Y

Y

G

G

D

D

L

L

P

H

H

S

P

T

D

D

E

D

V

-

N

-

T

-

E

-

L

-

P

-

L

F

F

F

T

T

E

E

T

T

A

P

Y

Y

A

A

P

P

S

S

S
|
S

P

P

Y
|
Y

S
|
S

A

A

S

S

K
|
K

A

A

S

S

D

D

H

H

L

L

V

V

R

R

A

A

W

W

K

L

R

R

T

T

Y

Y

G

G

L

L

P

P

T

T

I

L

V

I

T

T

N

N

C
|
C

S
|
S

N
|
N

Y

Y

G

G

P

P

Y

Y

H

H

F

F

P

P

E

E

K

K

L

L

I

I

P

P

L

L

V

M

I

I

L

L

N

N

A

A

L

L

E

A

G

G

K

K

A

S

L

L

P

P

I

V

Y

Y

G

G

K

N

G

G

D

Q

Q

Q

I

I

R

R

D

D

W

W

L

L

Y

Y

V

V

E

E

D

D

H

H

A

A

R

R

A

A

L

L

Y

Y

T

C

V

V

V

A

T

T

E

T

G

G

K

K

A

V

G

G

E

E

T

T

Y

Y

N

N

I

I

G

G

H

H

N

N

E

E

K

R

K

K

N

N

I

L

D

D

V

V

L

E

T

T

I

I

C

C

D

E

L

L

D

E

E

E

I

L

V

A

P

P

K

N

E

K

-

P

-

H

-

G

-

V

K

A

S

H

Y

Y

R

R

E

D

Q

L

I

I

T

T

Y

F

V

V

A

A

D

-

R

-

P

-

G

-

H

-

D

-

R

L

R

R

Y

Y

A

A

I

I

D

D

A

A

E

S

K

K

I

I

G

A

R

R

A

E

L

L

G

G

W

C

K

V

P

P

Q

Q

E

E

T

T

F

F

E

E

S

S

G

G

I

M

R

R

K

K

T

T

V

V

E

Q

W

W

Y

Y

L

L

S

A

N

N

T

E

K

S

W

W

V

W

D

K

N

Q

V

V

K

Q

S

D

G

G

A

S

Y

Y

Q

Q

active site: S125 (≠ N124), T134 (= T133), D135 (= D134), E136 (= E135), S158 (= S165), Y160 (= Y167), S161 (= S168), K164 (= K171)
binding nicotinamide-adenine-dinucleotide: G8 (= G7), A10 (= A9), G11 (= G10), F12 (= F11), I13 (= I12), D33 (= D32), K34 (= K33), L35 (= L34), T36 (= T35), A38 (= A37), G39 (= G38), V58 (≠ A57), D59 (= D58), I60 (= I59), L81 (= L80), A82 (= A81), A83 (= A82), T100 (= T99), I132 (= I131), S133 (= S132), T134 (= T133), Y160 (= Y167), K164 (= K171), C187 (= C194), S188 (= S195), N189 (= N196), N190 (= N197)

8du1A Crystal structure of NAD bound dtdp-glucose 4,6-dehydratase from elizabethkingia anophelis
55% identity, 98% coverage: 3:357/361 of query aligns to 6:360/361 of 8du1A

query
sites
8du1A

I

I

L

I

V

I

T

T

G
|
G

G

G

A
|
A

G
|
G

F
|
F

I
|
I

G

G

S

S

A

H

V

V

R

R

H

E

-

F

I

V

I

I

N

K

N

N

T

P

Q

E

D

I

S

T

V

I

N

N

V

L

D
|
D

K
x
A

L
|
L

T
|
T

Y

Y

A
|
A

G
|
G

N

N

R

L

E

E

S

N

L

L

A

K

D

D

V

I

S

E

D

N

S

F

E

P

R

N

Y

Y

V

V

F

F

E

E

H

K

A
|
A

D
|
D

I
|
I

C

T

D

K

A

P

P

E

A

E

M

L

A

R

R

K

I

V

F

F

A

E

Q

K

H

Y

Q

N

P

P

D

D

A

A

V

V

M

V

H

H

L
|
L

A
|
A

E

E

S

S

H

H

V

V

D

D

R

R

S

S

I

I

T

T

G

D

P

P

A

N

A

A

F

F

I

I

E

N

T
|
T

N

N

I

V

V

I

G

G

T

T

Y

A

V

N

L

L

E

N

A

L

A

C

R

R

N

E

Y

F

W

W

S

T

A

L

L

N

D

P

S

E

D

H

K

T

K

H

N

G

S

R

F

F

-

P

-

N

-

E

-

P

-

R

-

T

-

N

R

L

F

F

H

Y

H

H

I
x
V

S
|
S

T
|
T

D

D

E

E

V

V

Y

Y

G

G

D

S

L

L

P

-

H

-

P

-

D

-

E

-

V

-

N

-

T

-

E

G

E

E

L

T

P

G

L

F

F

F

T

L

E

E

T

T

A

A

Y

Y

A

D

P

P

S

Q

S

S

P

P

Y
|
Y

S

S

A

A

S

S

K
|
K

A

A

S

A

S

S

D

D

H

H

L

L

V

V

R

R

A

A

W

Y

K

G

R

N

T

T

Y

Y

G

G

L

M

P

P

T

F

I

I

V

V

T

S

N

N

C
|
C

S
|
S

N
|
N

Y

Y

G

G

P

P

Y

N

H

H

F

F

P

P

E

E

K

K

L

L

I

I

P

P

L

L

V

C

I

I

L

S

N

N

A

I

L

L

E

N

G

E

K

K

A

P

L

L

P

P

I

I

Y

Y

G

G

K

D

G

G

D

K

Q

Y

I

T

R

R

D

D

W

W

L

L

Y

Y

V

V

E

I

D

D

H

H

A

A

R

R

A

A

L

I

Y

H

T

Q

V

I

V

F

T

N

E

E

G

A

K

K

A

T

G

G

E

E

T

T

Y

Y

N

N

I

I

G

G

H

F

N

N

E

E

K

W

K

Q

N

N

I

I

D

D

V

L

V

V

L

K

T

E

I

L

C

I

D

K

L

Q

L

L

D

D

E

A

I

K

V

L

P

G

K

K

E

P

K

E

S

G

Y

H

R

S

E

E

Q

K

-

L

I

I

T

T

Y

F

V

V

A

K

D

D

R

R

P

P

G

G

H

H

D

D

R

K

R

R

Y

Y

A

A

I

I

D

D

A

A

E

T

K

K

I

L

G

N

R

K

A

D

L

L

G

G

W

W

K

K

P

P

Q

S

E

V

T

T

F

F

E

E

S

E

G

G

I

L

R

A

K

K

T

T

V

I

E

D

W

W

Y

Y

L

L

S

D

N

N

T

K

K

E

W

W

V

L

D

E

N

N

V

V

K

T

S

S

G

G

A

D

Y

Y

Q

Q

S

K

W

Y

I

Y

E

E

Q

N

N

Q

Y

Y

binding nicotinamide-adenine-dinucleotide: G10 (= G7), A12 (= A9), G13 (= G10), F14 (= F11), I15 (= I12), D36 (= D32), A37 (≠ K33), L38 (= L34), T39 (= T35), A41 (= A37), G42 (= G38), A61 (= A57), D62 (= D58), I63 (= I59), L84 (= L80), A85 (= A81), A86 (= A82), T103 (= T99), V142 (≠ I131), S143 (= S132), T144 (= T133), Y169 (= Y167), K173 (= K171), C196 (= C194), S197 (= S195), N198 (= N196), N199 (= N197)

2hunA Crystal structure of hypothetical protein ph0414 from pyrococcus horikoshii ot3
47% identity, 94% coverage: 1:340/361 of query aligns to 2:312/329 of 2hunA

query
sites
2hunA

M

M

K

K

I

L

L

L

V

V

T

T

G
|
G

G

G

A
x
M

G
|
G

F
|
F

I
|
I

G

G

S

S

A

N

V

F

V

I

R

R

H

Y

I

I

I

L

N

E

N

K

T

H

Q

P

D

D

-

W

S

E

V

V

V

I

N

N

V

I

D
|
D

K
|
K

L
|
L

T
x
G

Y
|
Y

A
x
G

G
x
S

N

N

R

P

E

A

S

N

L

L

A

K

D

D

V

L

S

E

D

D

S

D

E

P

R

R

Y

Y

V

T

F

F

E

V

H

K

A
x
G

D
|
D

I
x
V

C

A

D

D

A

Y

P

E

A

L

M

V

A

K

R

E

I

L

F

V

A

-

Q

-

H

R

Q

K

P

V

D

D

A

G

V

V

M

V

H

H

L
|
L

A
|
A

E

E

S
|
S

H

H

V

V

D

D

R

R

S

S

I

I

T

S

G

S

P

P

A

E

A

I

F

F

I

L

E

H

T
x
S

N

N

I

V

V

I

G

G

T

T

Y

Y

V

T

L

L

E

E

A

S

A

I

R

R

N

R

Y

-

W

-

S

-

A

-

L

-

D

-

S

-

D

-

K

E

K

N

N

P

S

E

F

V

R

R

F

F

H

V

H

H

I
x
V

S
|
S

T
|
T

D
|
D

E
|
E

V

V

Y

Y

G

G

D

D

L

I

P

L

H

K

P

G

D

S

E

-

V

-

N

-

T

-

E

-

L

-

P

-

L

-

F

F

T

T

E

E

T

N

T

D

A

R

Y

L

A

M

P

P

S

S

P

P

Y
|
Y

S

S

A

A

S

T

K
|
K

A

A

S

A

S

S

D

D

H

M

L

L

V

V

R

L

A

G

W

W

K

T

R

R

T

T

Y

Y

G

N

L

L

P

N

T

A

I

S

V

I

T

T

N

R

C
|
C

S
x
T

N
|
N

Y

Y

G

G

P

P

Y

Y

H

Q

F

F

P

P

E

E

K

K

L

L

I

I

P

P

L

K

V

T

I

I

L

I

N

R

A

A

L

S

E

L

G

G

K

L

A

K

L

I

P

P

I

I

Y

Y

G

G

K

-

G

-

D

-

Q

T

I

V

R

R

D

D

W

W

L

L

Y

Y

V

V

E

E

D

D

H

H

A

V

R

R

A

A

L

I

Y

E

T

L

V

V

V

L

T

L

E

K

G

G

K

E

A

S

G

R

E

E

T

I

Y

Y

N

N

I

I

G

S

G

A

H

G

N

E

E

E

K

K

K

T

N

N

I

L

D

E

V

V

L

K

T

I

I

I

C

L

D

R

L

L

L

M

D

G

E

K

I

-

V

-

P

-

K

-

E

-

K

-

S

-

Y

G

R

E

E

E

Q

L

I

I

T

E

Y

L

V

V

A

E

D

D

R

R

P

P

G

G

H

H

D

D

R

L

R

R

Y

Y

A

S

I

L

D

D

A

S

E

W

K

K

I

I

G

T

R

R

A

D

L

L

G

K

W

W

K

R

P

P

Q

K

E

Y

T

T

F

F

E

D

S

E

G

G

I

I

R

K

K

K

T

T

V

I

E

D

W

W

Y

Y

L

L

S

K

N

N

T

E

K

W

W

W

active site: T125 (= T133), D126 (= D134), E127 (= E135), Y149 (= Y167), K153 (= K171)
binding nicotinamide-adenine-dinucleotide: G8 (= G7), M10 (≠ A9), G11 (= G10), F12 (= F11), I13 (= I12), D34 (= D32), K35 (= K33), L36 (= L34), G37 (≠ T35), Y38 (= Y36), G39 (≠ A37), S40 (≠ G38), G59 (≠ A57), D60 (= D58), V61 (≠ I59), L80 (= L80), A81 (= A81), A82 (= A82), S84 (= S84), S99 (≠ T99), V123 (≠ I131), S124 (= S132), T125 (= T133), Y149 (= Y167), K153 (= K171), C176 (= C194), T177 (≠ S195), N178 (= N196), N179 (= N197)

P95780 dTDP-glucose 4,6-dehydratase; EC 4.2.1.46 from Streptococcus mutans serotype c (strain ATCC 700610 / UA159) (see paper)
45% identity, 99% coverage: 3:361/361 of query aligns to 7:344/348 of P95780

query
sites
P95780

I

I

L

I

V

V

T

T

G

G

A

A

G

G

F
|
F

I
|
I

G

G

S

S

A

N

V

F

V

V

R

H

H

Y

I

V

I

Y

N

N

T

H

Q

P

D

D

S

V

V

H

V

V

N

T

V

V

-

L

D
|
D

K
|
K

L
|
L

T
|
T

Y

Y

A

A

G

G

N

N

R

R

E

A

S

N

L

L

A

E

D

E

V

I

S

L

D

-

S

G

E

D

R

R

Y

V

V

E

F

L

E

V

H

V

A

G

D
|
D

I
|
I

C

A

D

D

A

S

P

E

A

L

M

V

A

D

R

K

I

L

F

A

A

A

Q

K

H

-

Q

-

P

A

D

D

A

A

V

I

M

V

H

H

L
x
Y

A
|
A

E
|
E

S
|
S

H

H

V
x
N

D

D

R

N

S

S

I

L

T

K

G

D

P

P

A

S

A

P

F

F

I

I

E

Y

T
|
T

N

N

I

F

V

V

G

G

T

T

Y

Y

V

I

L

L

E

E

A

A

R

R

N

K

Y

Y

W

-

S

-

A

-

L

-

D

-

S

-

D

-

K

-

N

-

S

D

F

I

R

R

F

F

H

H

I

V

S

S

T
|
T

D

D

E

E

V

V

Y

Y

G

G

D

D

L

L

P

P

H

L

P

R

D

E

E

D

V

L

N

P

N

G

T

H

E

G

E

E

L

G

P

P

-

G

-

E

L

K

F

F

T

T

E

A

T

E

T

T

A

K

Y

Y

A

N

P

P

S

S

P

P

Y

Y

S

S

A

S

S

T

K

K

A

A

S

A

S

S

D

D

H

L

L

I

V

V

R

K

A

A

W

W

K

V

R

R

T

S

Y

F

G

G

L

V

P

K

T

A

I

T

V

I

T

S

N

N

C

C

S

S

N
|
N

Y

Y

G

G

P

P

Y

Y

H

Q

F

H

P

I

E

E

K
|
K

L
x
F

I
|
I

P
|
P

L
x
R

V
x
Q

I

I

L

T

N

N

A

I

L

L

E

S

G

G

K

I

A

K

L

P

P
x
K

I
x
L

Y
|
Y

G

G

K

E

G

G

D

K

Q

N

I

V

R
|
R

D

D

W

W

L

I

Y

H

V

T

E

N

D

D

H

H

A

S

R

T

A

G

L

V

Y

W

T

A

V

I

V

L

T

T

E

K

G

G

K

R

A

I

G

G

E

E

T

T

Y

Y

N

L

I

I

G

G

G

A

H

D

N

G

E

E

K

K

K

N

N
|
N

I

K

D

E

V

V

V

-

L

-

T

-

I

-

C

-

D

-

L

-

L

L

D

E

E

L

I

I

V

L

P

E

K

K

E

M

K

S

S

Q

Y

P

R

K

E

N

Q

A

I

Y

T

D

Y

H

V

V

A

T

D

D

R

R

P

A

G

G

H

H

D

D

R

L

R

R

Y

Y

A

A

I

I

D

D

A

S

E

T

K

K

I

L

G

R

R

E

A

E

L

L

G

G

W

W

K

K

P

P

Q

Q

E

F

T

T

-

N

F

F

E

E

S

E

G

G

I

L

R

E

K

D

T

T

V

I

E

K

W

W

Y

Y

L

T

S

E

N

H

T

E

K

D

W

W

V

W

D

K

N

A

V

E

K

K

S

E

G

A

A

-

Y

-

Q

-

S

-

W

-

I

V

E

E

Q

A

N

N

Y

Y

E

A

G

K

R

T

Q

Q

FI 15:16 (= FI 11:12) binding
DKLT 37:40 (= DKLT 32:35) binding
DI 62:63 (= DI 58:59) binding
YAAES 82:86 (≠ LAAES 80:84) binding
N88 (≠ V86) binding
T101 (= T99) binding
T125 (= T133) binding
N190 (= N196) binding
N191 (= N197) binding
KFIPRQ 200:205 (≠ KLIPLV 206:211) binding
KLY 216:218 (≠ PIY 222:224) binding
R225 (= R231) binding
N260 (= N266) binding

1kerB The crystal structure of dtdp-d-glucose 4,6-dehydratase (rmlb) from streptococcus suis with dtdp-d-glucose bound (see paper)
45% identity, 99% coverage: 3:361/361 of query aligns to 6:343/347 of 1kerB

query
sites
1kerB

I

I

L

I

V

V

T

T

G
|
G

G

G

A
|
A

G
|
G

F
|
F

I
|
I

G

G

S

S

A

N

V

F

V

V

R

H

H

Y

I

V

I

Y

N

N

T

H

Q

P

D

D

S

V

V

H

V

V

N

T

V

V

-
x
L

D
|
D

K
|
K

L
|
L

T
|
T

Y

Y

A
|
A

G
|
G

N

N

R

K

E

A

S

N

L

L

A

E

D

A

V

I

S

L

D

-

S

G

E

D

R

R

Y

V

V

E

F

L

E

V

H

V

A
x
G

D
|
D

I
|
I

C

A

D

D

A

A

P

E

A

L

M

V

A

D

R

K

I

L

F

A

A

A

Q

K

H

-

Q

-

P

A

D

D

A

A

V

I

M

V

H

H

L
x
Y

A
|
A

E

E

S
|
S

H
|
H

V
x
N

D

D

R

N

S

S

I

L

T

N

G

D

P

P

A

S

A

P

F

F

I

I

E

H

T
|
T

N

N

I

F

V

I

G

G

T

T

Y

Y

V

T

L

L

E

E

A

A

R

R

N

K

Y

Y

W

-

S

-

A

-

L

-

D

-

S

-

D

-

K

-

N

-

S

D

F

I

R

R

F

F

H

H

I
x
V

S
|
S

T
|
T

D
|
D

E
|
E

V

V

Y

Y

G

G

D

D

L

L

P

P

H

L

P

R

D

E

E

D

V

L

N

P

N

G

T

H

E

G

E

E

L

G

P

P

-

G

-

E

L

K

F

F

T

T

E

A

T

E

T

T

A

N

Y

Y

A

N

P

P

S

S

P

P

Y
|
Y

S

S

A

S

S

T

K
|
K

A

A

S

A

S

S

D

D

H

L

L

I

V

V

R

K

A

A

W

W

K

V

R

R

T

S

Y

F

G

G

L

V

P

K

T

A

I

T

V

I

T

S

N

N

C
|
C

S
|
S

N
|
N

Y

Y

G

G

P

P

Y

Y

H

Q

F

H

P

I

E

E

K
|
K

L
x
F

I

I

P

P

L
x
R

V
x
Q

I

I

L

T

N

N

A

I

L

L

E

A

G

G

K

I

A

K

L

P

P
x
K

I
x
L

Y
|
Y

G

G

K

E

G

G

D

K

Q

N

I

V

R
|
R

D

D

W

W

L

I

Y

H

V

T

E

N

D

D

H

H

A

S

R

T

A

G

L

V

Y

W

T

A

V

I

V

L

T

T

E

K

G

G

K

R

A

M

G

G

E

E

T

T

Y

Y

N

L

I

I

G

G

G

A

H

D

N

G

E

E

K

K

K

N

N
|
N

I

K

D

E

V

V

V

-

L

-

T

-

I

-

C

-

D

-

L

-

L

L

D

E

E

L

I

I

V

L

P

E

K

K

E

M

K

G

S

Q

Y

P

R

K

E

D

Q

A

I

Y

T

D

Y

H

V

V

A

T

D

D

R
|
R

P

A

G

G

H
|
H

D

D

R

L

R

R

Y
|
Y

A

A

I

I

D

D

A

A

E

S

K

K

I

L

G

R

R

D

A

E

L

L

G

G

W

W

K

T

P

P

Q

Q

E

F

T

T

-

D

F

F

E

S

S

E

G

G

I

L

R

E

K

E

T

T

V

I

E

Q

W

W

Y

Y

L

T

S

D

N

N

T

Q

K

D

W

W

V

W

D

K

N

A

V

E

K

K

S

E

G

A

A

-

Y

-

Q

-

S

-

W

-

I

V

E

E

Q

A

N

N

Y
|
Y

E

A

G

K

R

T

Q

Q

active site: T124 (= T133), D125 (= D134), E126 (= E135), Y160 (= Y167), K164 (= K171)
binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: S85 (= S84), H86 (= H85), N87 (≠ V86), T124 (= T133), D125 (= D134), E126 (= E135), Y160 (= Y167), C187 (= C194), S188 (= S195), N189 (= N196), K199 (= K206), F200 (≠ L207), R203 (≠ L210), Q204 (≠ V211), K215 (≠ P222), L216 (≠ I223), Y217 (= Y224), R224 (= R231), N259 (= N266), R283 (= R297), H286 (= H300), Y290 (= Y304), Y339 (= Y357)
binding nicotinamide-adenine-dinucleotide: G10 (= G7), A12 (= A9), G13 (= G10), F14 (= F11), I15 (= I12), L35 (vs. gap), D36 (= D32), K37 (= K33), L38 (= L34), T39 (= T35), A41 (= A37), G42 (= G38), G60 (≠ A57), D61 (= D58), I62 (= I59), Y81 (≠ L80), A82 (= A81), A83 (= A82), S85 (= S84), T100 (= T99), V122 (≠ I131), S123 (= S132), T124 (= T133), Y160 (= Y167), K164 (= K171), C187 (= C194), S188 (= S195), N189 (= N196), N190 (= N197), K199 (= K206)

1ketA The crystal structure of dtdp-d-glucose 4,6-dehydratase (rmlb) from streptococcus suis with thymidine diphosphate bound (see paper)
45% identity, 99% coverage: 3:361/361 of query aligns to 5:342/346 of 1ketA

query
sites
1ketA

I

I

L

I

V

V

T

T

G
|
G

G

G

A
|
A

G
|
G

F
|
F

I
|
I

G

G

S

S

A

N

V

F

V

V

R

H

H

Y

I

V

I

Y

N

N

T

H

Q

P

D

D

S

V

V

H

V

V

N

T

V

V

-

L

D
|
D

K
|
K

L
|
L

T
|
T

Y

Y

A
|
A

G
|
G

N

N

R

K

E

A

S

N

L

L

A

E

D

A

V

I

S

L

D

-

S

G

E

D

R

R

Y

V

V

E

F

L

E

V

H

V

A
x
G

D
|
D

I
|
I

C

A

D

D

A

A

P

E

A

L

M

V

A

D

R

K

I

L

F

A

A

A

Q

K

H

-

Q

-

P

A

D

D

A

A

V

I

M

V

H

H

L
x
Y

A
|
A

E

E

S
|
S

H
|
H

V
x
N

D

D

R

N

S

S

I

L

T

N

G

D

P

P

A

S

A

P

F

F

I

I

E

H

T
|
T

N

N

I

F

V

I

G

G

T

T

Y

Y

V

T

L

L

E

E

A

A

R

R

N

K

Y

Y

W

-

S

-

A

-

L

-

D

-

S

-

D

-

K

-

N

-

S

D

F

I

R

R

F

F

H

H

I
x
V

S
|
S

T
|
T

D
|
D

E
|
E

V

V

Y

Y

G

G

D

D

L

L

P

P

H

L

P

R

D

E

E

D

V

L

N

P

N

G

T

H

E

G

E

E

L

G

P

P

-

G

-

E

L

K

F

F

T

T

E

A

T

E

T

T

A

N

Y

Y

A

N

P

P

S

S

P

P

Y
|
Y

S

S

A

S

S

T

K
|
K

A

A

S

A

S

S

D

D

H

L

L

I

V

V

R

K

A

A

W

W

K

V

R

R

T

S

Y

F

G

G

L

V

P

K

T

A

I

T

V

I

T

S

N

N

C
|
C

S
|
S

N
|
N

Y

Y

G

G

P

P

Y

Y

H

Q

F

H

P

I

E

E

K
|
K

L
x
F

I

I

P

P

L
x
R

V
x
Q

I

I

L

T

N

N

A

I

L

L

E

A

G

G

K

I

A

K

L

P

P
x
K

I
x
L

Y
|
Y

G

G

K

E

G

G

D

K

Q

N

I

V

R
|
R

D

D

W

W

L

I

Y

H

V

T

E

N

D

D

H

H

A

S

R

T

A

G

L

V

Y

W

T

A

V

I

V

L

T

T

E

K

G

G

K

R

A

M

G

G

E

E

T

T

Y

Y

N

L

I

I

G

G

G

A

H

D

N

G

E

E

K

K

K

N

N
|
N

I

K

D

E

V

V

V

-

L

-

T

-

I

-

C

-

D

-

L

-

L

L

D

E

E

L

I

I

V

L

P

E

K

K

E

M

K

G

S

Q

Y

P

R

K

E

D

Q

A

I

Y

T

D

Y

H

V

V

A

T

D

D

R
|
R

P

A

G

G

H
|
H

D

D

R

L

R

R

Y

Y

A

A

I

I

D

D

A

A

E

S

K

K

I

L

G

R

R

D

A

E

L

L

G

G

W

W

K

T

P

P

Q

Q

E

F

T

T

-

D

F

F

E

S

S

E

G

G

I

L

R

E

K

E

T

T

V

I

E

Q

W

W

Y

Y

L

T

S

D

N

N

T

Q

K

D

W

W

V

W

D

K

N

A

V

E

K

K

S

E

G

A

A

-

Y

-

Q

-

S

-

W

-

I

V

E

E

Q

A

N

N

Y

Y

E

A

G

K

R

T

Q

Q

active site: T123 (= T133), D124 (= D134), E125 (= E135), Y159 (= Y167), K163 (= K171)
binding nicotinamide-adenine-dinucleotide: G9 (= G7), A11 (= A9), G12 (= G10), F13 (= F11), I14 (= I12), D35 (= D32), K36 (= K33), L37 (= L34), T38 (= T35), A40 (= A37), G41 (= G38), G59 (≠ A57), D60 (= D58), I61 (= I59), Y80 (≠ L80), A81 (= A81), A82 (= A82), S84 (= S84), T99 (= T99), V121 (≠ I131), S122 (= S132), T123 (= T133), Y159 (= Y167), K163 (= K171), C186 (= C194), S187 (= S195), N188 (= N196), N189 (= N197), K198 (= K206)
binding thymidine-5'-diphosphate: H85 (= H85), N86 (≠ V86), E125 (= E135), N188 (= N196), K198 (= K206), F199 (≠ L207), R202 (≠ L210), Q203 (≠ V211), K214 (≠ P222), L215 (≠ I223), Y216 (= Y224), R223 (= R231), N258 (= N266), R282 (= R297), H285 (= H300)

1kepA The crystal structure of dtdp-d-glucose 4,6-dehydratase (rmlb) from streptococcus suis with dtdp-xylose bound (see paper)
45% identity, 99% coverage: 3:361/361 of query aligns to 5:342/346 of 1kepA

query
sites
1kepA

I

I

L

I

V

V

T

T

G
|
G

G

G

A
|
A

G
|
G

F
|
F

I
|
I

G

G

S

S

A

N

V

F

V

V

R

H

H

Y

I

V

I

Y

N

N

T

H

Q

P

D

D

S

V

V

H

V

V

N

T

V

V

-

L

D
|
D

K
|
K

L
|
L

T
|
T

Y

Y

A
|
A

G
|
G

N

N

R

K

E

A

S

N

L

L

A

E

D

A

V

I

S

L

D

-

S

G

E

D

R

R

Y

V

V

E

F

L

E

V

H

V

A
x
G

D
|
D

I
|
I

C

A

D

D

A

A

P

E

A

L

M

V

A

D

R

K

I

L

F

A

A

A

Q

K

H

-

Q

-

P

A

D

D

A

A

V

I

M

V

H

H

L
x
Y

A
|
A

E

E

S
|
S

H

H

V
x
N

D

D

R

N

S

S

I

L

T

N

G

D

P

P

A

S

A

P

F

F

I

I

E

H

T
|
T

N

N

I

F

V

I

G

G

T

T

Y

Y

V

T

L

L

E

E

A

A

R

R

N

K

Y

Y

W

-

S

-

A

-

L

-

D

-

S

-

D

-

K

-

N

-

S

D

F

I

R

R

F

F

H

H

I
x
V

S
|
S

T
|
T

D
|
D

E
|
E

V

V

Y

Y

G

G

D

D

L

L

P

P

H

L

P

R

D

E

E

D

V

L

N

P

N

G

T

H

E

G

E

E

L

G

P

P

-

G

-

E

L

K

F

F

T

T

E

A

T

E

T

T

A

N

Y

Y

A

N

P

P

S

S

P

P

Y
|
Y

S

S

A

S

S

T

K
|
K

A

A

S

A

S

S

D

D

H

L

L

I

V

V

R

K

A

A

W

W

K

V

R

R

T

S

Y

F

G

G

L

V

P

K

T

A

I

T

V

I

T

S

N

N

C
|
C

S
|
S

N
|
N

Y

Y

G

G

P

P

Y

Y

H

Q

F

H

P

I

E

E

K
|
K

L

F

I

I

P

P

L

R

V

Q

I

I

L

T

N

N

A

I

L

L

E

A

G

G

K

I

A

K

L

P

P

K

I

L

Y

Y

G

G

K

E

G

G

D

K

Q

N

I

V

R
|
R

D

D

W

W

L

I

Y

H

V

T

E

N

D

D

H

H

A

S

R

T

A

G

L

V

Y

W

T

A

V

I

V

L

T

T

E

K

G

G

K

R

A

M

G

G

E

E

T

T

Y

Y

N

L

I

I

G

G

G

A

H

D

N

G

E

E

K

K

K

N

N

N

I

K

D

E

V

V

V

-

L

-

T

-

I

-

C

-

D

-

L

-

L

L

D

E

E

L

I

I

V

L

P

E

K

K

E

M

K

G

S

Q

Y

P

R

K

E

D

Q

A

I

Y

T

D

Y

H

V

V

A

T

D

D

R
|
R

P

A

G

G

H

H

D

D

R

L

R

R

Y

Y

A

A

I

I

D

D

A

A

E

S

K

K

I

L

G

R

R

D

A

E

L

L

G

G

W

W

K

T

P

P

Q

Q

E

F

T

T

-

D

F

F

E

S

S

E

G

G

I

L

R

E

K

E

T

T

V

I

E

Q

W

W

Y

Y

L

T

S

D

N

N

T

Q

K

D

W

W

V

W

D

K

N

A

V

E

K

K

S

E

G

A

A

-

Y

-

Q

-

S

-

W

-

I

V

E

E

Q

A

N

N

Y

Y

E

A

G

K

R

T

Q

Q

active site: T123 (= T133), D124 (= D134), E125 (= E135), Y159 (= Y167), K163 (= K171)
binding nicotinamide-adenine-dinucleotide: G9 (= G7), A11 (= A9), G12 (= G10), F13 (= F11), I14 (= I12), D35 (= D32), K36 (= K33), L37 (= L34), T38 (= T35), A40 (= A37), G41 (= G38), G59 (≠ A57), D60 (= D58), I61 (= I59), Y80 (≠ L80), A81 (= A81), A82 (= A82), S84 (= S84), T99 (=