SitesBLAST
Comparing 16354 b2247 predicted enolase (RefSeq) to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P77215 L-rhamnonate dehydratase; RhamD; EC 4.2.1.90 from Escherichia coli (strain K12) (see paper)
100% identity, 100% coverage: 1:401/401 of query aligns to 1:401/401 of P77215
- H29 (= H29) mutation to N: Loss of L-rhamnonate dehydratase activity due to absence of substrate binding.
- H277 (= H277) mutation to N: 35-fold decrease in L-rhamnonate dehydratase activity. 59-fold decrease in substrate affinity.
- H325 (= H325) mutation to N: Loss of L-rhamnonate dehydratase activity. 2-fold decrease in substrate affinity.
3cxoB Crystal structure of l-rhamnonate dehydratase from salmonella typhimurium complexed with mg and 3-deoxy-l-rhamnonate (see paper)
96% identity, 100% coverage: 1:401/401 of query aligns to 2:402/402 of 3cxoB
- active site: Y52 (= Y51), K186 (= K185), P188 (= P187), G192 (= G191), H194 (= H193), A198 (= A197), D223 (= D222), W225 (= W224), E249 (= E248), T274 (= T273), G276 (= G275), E277 (= E276), Q297 (= Q296), D299 (= D298), H326 (= H325), E346 (= E345), S351 (= S350)
- binding (2R,4S)-2,4,7-trihydroxyheptanoic acid: H30 (= H29), I42 (= I41), R56 (= R55), K186 (= K185), P188 (= P187), D223 (= D222), W225 (= W224), E277 (= E276), H326 (= H325), E346 (= E345)
- binding magnesium ion: D223 (= D222), E249 (= E248), E277 (= E276)
3cxoA Crystal structure of l-rhamnonate dehydratase from salmonella typhimurium complexed with mg and 3-deoxy-l-rhamnonate (see paper)
96% identity, 100% coverage: 1:401/401 of query aligns to 2:402/402 of 3cxoA
- active site: Y52 (= Y51), K186 (= K185), P188 (= P187), G192 (= G191), H194 (= H193), A198 (= A197), D223 (= D222), W225 (= W224), E249 (= E248), T274 (= T273), G276 (= G275), E277 (= E276), Q297 (= Q296), D299 (= D298), H326 (= H325), E346 (= E345), S351 (= S350)
- binding 3,6-dideoxy-L-arabino-hexonic acid: W37 (= W36), R56 (= R55), K186 (= K185), D223 (= D222), W225 (= W224), E277 (= E276), H326 (= H325), E346 (= E345)
- binding magnesium ion: D223 (= D222), E249 (= E248), E277 (= E276)
3d46A Crystal structure of l-rhamnonate dehydratase from salmonella typhimurium complexed with mg and l-tartrate
96% identity, 100% coverage: 1:401/401 of query aligns to 5:405/405 of 3d46A
- active site: K189 (= K185), P191 (= P187), G195 (= G191), H197 (= H193), A201 (= A197), D226 (= D222), W228 (= W224), E252 (= E248), T277 (= T273), E280 (= E276), Q300 (= Q296), D302 (= D298), H329 (= H325), E349 (= E345), P362 (= P358)
- binding magnesium ion: D226 (= D222), E252 (= E248), E280 (= E276)
3ekgA Crystal structure of l-rhamnonate dehydratase from azotobacter vinelandii complexed with mg and l-tartrate
63% identity, 100% coverage: 1:401/401 of query aligns to 2:395/395 of 3ekgA
- active site: K178 (= K185), P180 (= P187), G184 (= G191), S186 (≠ H193), E190 (≠ A197), D215 (= D222), W217 (= W224), E241 (= E248), T266 (= T273), G268 (= G275), E269 (= E276), Q290 (= Q296), D292 (= D298), H319 (= H325), E339 (= E345), D348 (≠ S354)
- binding magnesium ion: D215 (= D222), E241 (= E248), E269 (= E276)
3fxgA Crystal structure of rhamnonate dehydratase from gibberella zeae complexed with mg
44% identity, 98% coverage: 3:396/401 of query aligns to 7:395/407 of 3fxgA
- active site: K184 (= K185), P186 (= P187), E196 (≠ A197), D221 (= D222), Y223 (≠ W224), E247 (= E248), G273 (= G275), E274 (= E276), Q295 (= Q296), D297 (= D298), H324 (= H325), E344 (= E345)
- binding magnesium ion: D221 (= D222), E247 (= E248), E274 (= E276)
3stpA Crystal structure of a putative galactonate dehydratase
30% identity, 81% coverage: 66:390/401 of query aligns to 48:379/390 of 3stpA
- active site: L66 (≠ T83), S69 (≠ E86), S151 (≠ T169), K177 (= K185), R179 (≠ P187), P189 (≠ A197), E214 (≠ D222), Y216 (≠ W224), E240 (= E248), G265 (= G275), E266 (= E276), H267 (= H277), Q287 (= Q296), D289 (= D298), I311 (≠ Q320), H316 (= H325), E336 (= E345), F341 (≠ S350)
- binding magnesium ion: E214 (≠ D222), E240 (= E248), E266 (= E276)
3sqsA Crystal structure of a putative mandelate racemase/muconate lactonizing protein from dinoroseobacter shibae dfl 12
29% identity, 83% coverage: 66:396/401 of query aligns to 44:380/386 of 3sqsA
- active site: L62 (≠ T83), N65 (≠ E86), S147 (vs. gap), K173 (= K185), R175 (≠ P187), G177 (≠ H189), G179 (= G191), K181 (≠ H193), A185 (= A197), E210 (≠ D222), Y212 (≠ W224), E236 (= E248), G261 (= G275), E262 (= E276), H263 (= H277), Q283 (= Q296), D285 (= D298), I307 (≠ Q320), H312 (= H325), G314 (≠ S327), E332 (= E345), F337 (≠ S350)
- binding magnesium ion: E210 (≠ D222), E236 (= E248), E262 (= E276), Y347 (≠ D361), F350 (≠ L364), D351 (≠ L365)
4hpnA Crystal structure of a proposed galactarolactone cycloisomerase from agrobacterium tumefaciens, target efi-500704, with bound ca, ordered loops (see paper)
28% identity, 90% coverage: 34:393/401 of query aligns to 8:369/378 of 4hpnA
- active site: F19 (≠ Y51), G50 (≠ A84), R53 (≠ M87), T134 (= T169), K164 (= K185), K166 (≠ P187), D194 (= D222), N196 (≠ W224), E220 (= E248), G245 (= G275), E246 (= E276), T247 (≠ H277), Q267 (= Q296), D269 (= D298), H296 (= H325), V297 (vs. gap), W298 (vs. gap), R320 (≠ M348), E329 (≠ R357), F330 (vs. gap), H334 (vs. gap)
- binding calcium ion: D194 (= D222), D209 (≠ H237), E220 (= E248), G237 (≠ N265), E246 (= E276)
3sjnA Crystal structure of enolase spea_3858 (target efi-500646) from shewanella pealeana with magnesium bound
30% identity, 74% coverage: 96:392/401 of query aligns to 67:365/373 of 3sjnA
- active site: T139 (= T169), K165 (= K185), G167 (≠ P187), M171 (≠ G191), D198 (= D222), A200 (vs. gap), E225 (= E248), I247 (≠ V272), G250 (= G275), E251 (= E276), S252 (≠ H277), Q272 (= Q296), D274 (= D298), H301 (= H325), G302 (= G326), F303 (vs. gap), M325 (= M348), E326 (≠ T349), Q329 (≠ D352), S331 (= S354)
- binding magnesium ion: D198 (= D222), E225 (= E248), E251 (= E276)
Sites not aligning to the query:
3n4eA Crystal structure of mandelate racemase/muconate lactonizing protein from paracoccus denitrificans pd1222
29% identity, 81% coverage: 68:392/401 of query aligns to 21:355/368 of 3n4eA
- active site: S124 (≠ T169), K153 (vs. gap), R155 (≠ H189), V165 (≠ A197), D190 (= D222), N192 (≠ W224), E216 (= E248), G241 (= G275), E242 (= E276), Q262 (= Q296), D264 (= D298), H291 (= H325), Q292 (≠ G326), T293 (≠ S327), G296 (≠ Y330), P315 (≠ C353), V316 (≠ S354), E317 (≠ T355), L318 (≠ M356), R324 (vs. gap)
- binding calcium ion: D190 (= D222), E216 (= E248), E242 (= E276), V352 (≠ L389), E354 (≠ R391)
3ck5A Crystal structure of a racemase from streptomyces coelicolor a3(2) with bound magnesium
29% identity, 81% coverage: 66:390/401 of query aligns to 32:348/357 of 3ck5A
- active site: T50 (≠ A80), G137 (vs. gap), K164 (= K185), K166 (≠ P187), D195 (= D222), N197 (≠ W224), I220 (= I247), E221 (= E248), I243 (≠ V272), G246 (= G275), E247 (= E276), E268 (≠ Q296), D270 (= D298), H297 (= H325), G298 (= G326), V299 (≠ S327), Y315 (≠ F343), E317 (= E345)
- binding magnesium ion: D195 (= D222), E221 (= E248), E247 (= E276)
Sites not aligning to the query:
Q9RKF7 3,6-anhydro-alpha-L-galactonate cycloisomerase; AHGA cycloisomerase; EC 5.5.1.25 from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
29% identity, 70% coverage: 66:345/401 of query aligns to 32:317/361 of Q9RKF7
- D195 (= D222) binding
- E221 (= E248) binding
- E247 (= E276) binding
5olcC Crystal structure of the 3,6-anhydro-d-galactonate cycloisomerase from zobellia galactanivorans (see paper)
29% identity, 73% coverage: 99:392/401 of query aligns to 55:343/351 of 5olcC
- active site: K148 (= K185), K150 (≠ P187), D178 (= D222), N180 (≠ W224), E204 (= E248), G229 (= G275), E230 (= E276), D253 (= D298), H280 (= H325), E304 (≠ L347), E309 (≠ D352)
- binding magnesium ion: D178 (= D222), E204 (= E248), E230 (= E276)
4girB Crystal structure of an enolase family member from vibrio harveyi (efi-target 501692) with homology to mannonate dehydratase, with mg, ethylene glycol and sulfate bound (ordered loops, space group p41212)
26% identity, 84% coverage: 66:401/401 of query aligns to 34:393/411 of 4girB
- active site: T51 (= T83), Q54 (≠ E86), W92 (≠ Y122), R163 (vs. gap), Q165 (≠ E178), D217 (= D222), H219 (≠ W224), E243 (= E248), G268 (= G275), E269 (= E276), L270 (≠ H277), R290 (≠ Q296), H292 (≠ D298), H319 (vs. gap), P321 (vs. gap), E346 (= E345)
- binding magnesium ion: D217 (= D222), E243 (= E248), E269 (= E276)
Sites not aligning to the query:
D0X4R4 D-galactonate dehydratase family member VME_00770 from Vibrio harveyi (strain 1DA3) (see paper)
26% identity, 84% coverage: 66:401/401 of query aligns to 22:381/399 of D0X4R4
- D205 (= D222) binding
- E231 (= E248) binding
- E257 (= E276) binding
3sbfA Crystal structure of the mutant p311a of enolase superfamily member from vibrionales bacterium complexed with mg and d-arabinonate
26% identity, 83% coverage: 66:398/401 of query aligns to 20:376/397 of 3sbfA
- active site: F38 (≠ A84), R41 (≠ M87), Y122 (= Y167), R149 (vs. gap), Q151 (≠ E178), D203 (= D222), H205 (≠ W224), E229 (= E248), G254 (= G275), E255 (= E276), L256 (≠ H277), R276 (≠ Q296), H278 (≠ D298), H305 (vs. gap), A307 (vs. gap), E332 (= E345), N337 (≠ S350)
- binding D-arabinonic acid: D203 (= D222), H205 (≠ W224), E255 (= E276), H305 (vs. gap), E332 (= E345)
- binding magnesium ion: D203 (= D222), E229 (= E248), E255 (= E276)
Sites not aligning to the query:
3r25B Crystal structure of enolase superfamily member from vibrionales bacterium complexed with mg and glycerol in the active site
26% identity, 83% coverage: 66:398/401 of query aligns to 22:378/399 of 3r25B
- active site: F40 (≠ A84), R43 (≠ M87), Y124 (= Y167), R151 (vs. gap), Q153 (≠ E178), D205 (= D222), H207 (≠ W224), E231 (= E248), G256 (= G275), E257 (= E276), L258 (≠ H277), R278 (≠ Q296), H280 (≠ D298), H307 (vs. gap), P309 (vs. gap), E334 (= E345), N339 (≠ S350)
- binding magnesium ion: D205 (= D222), E231 (= E248), E257 (= E276)
Sites not aligning to the query:
A5KUH4 D-galactonate dehydratase family member VSWAT3_13707 from Vibrionales bacterium (strain SWAT-3) (see paper)
26% identity, 83% coverage: 66:398/401 of query aligns to 22:378/399 of A5KUH4
- D205 (= D222) binding
- E231 (= E248) binding
- E257 (= E276) binding
3cb3A Crystal structure of l-talarate dehydratase from polaromonas sp. Js666 complexed with mg and l-glucarate
28% identity, 87% coverage: 41:390/401 of query aligns to 16:357/373 of 3cb3A
- active site: K24 (≠ R49), S57 (vs. gap), T143 (= T169), K171 (= K185), K173 (≠ P187), D202 (= D222), N204 (≠ W224), E228 (= E248), G253 (= G269), E254 (≠ M270), M275 (≠ Q296), D277 (= D298), H304 (= H325), F305 (≠ G326), A306 (≠ S327), E324 (= E345)
- binding l-glucaric acid: K171 (= K185), K173 (≠ P187), D202 (= D222), E254 (≠ M270), H304 (= H325)
- binding magnesium ion: D202 (= D222), E228 (= E248), A243 (≠ E261), F246 (≠ R264), E254 (≠ M270)
Query Sequence
>16354 b2247 predicted enolase (RefSeq)
MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATPMSKYRDYEQSRQSFGI
NVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLSATL
YYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAVRDEIQFYATGARPDLAKEMG
FIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFWLMLDCWMSQDVNYATKLAHACA
PYNLKWIEECLPPQQYESYRELKRNAPVGMMVTSGEHHGTLQSFRTLSETGIDIMQPDVG
WCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTMRPQF
DPILLNEPVPVNGRIHKSVLDKPGFGVELNRDCNLKRPYSH
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory