SitesBLAST
Comparing 16426 FitnessBrowser__Keio:16426 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P23247 Aspartate-semialdehyde dehydrogenase 2; ASA dehydrogenase 2; ASADH 2; Aspartate-beta-semialdehyde dehydrogenase 2; EC 1.2.1.11 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see paper)
48% identity, 100% coverage: 1:336/337 of query aligns to 1:337/337 of P23247
- C132 (≠ S132) active site, Acyl-thioester intermediate
2r00C Crystal structure of aspartate semialdehyde dehydrogenase ii complexed with asa from vibrio cholerae (see paper)
47% identity, 99% coverage: 2:336/337 of query aligns to 1:336/336 of 2r00C
Q04797 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Bacillus subtilis (strain 168) (see paper)
33% identity, 98% coverage: 1:331/337 of query aligns to 1:340/346 of Q04797
- S98 (≠ G98) modified: Phosphoserine
- Y146 (≠ G148) modified: Phosphotyrosine
3pylC Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with d-2,3-diaminopropionate (see paper)
33% identity, 97% coverage: 4:331/337 of query aligns to 1:340/361 of 3pylC
4r51A Crystal complex structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide adenine dinucleotide phosphate and phthalic acid (see paper)
33% identity, 97% coverage: 4:331/337 of query aligns to 1:340/360 of 4r51A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), V13 (= V16), A35 (= A38), S36 (≠ R39), S39 (= S42), T56 (≠ A59), S70 (≠ V73), A71 (= A74), G72 (= G75), N93 (≠ S96), T94 (≠ S97), N126 (≠ D131), C127 (≠ S132), G160 (= G165), M161 (≠ K166), G328 (= G319)
- binding phthalic acid: S73 (≠ K76), T94 (≠ S97), S95 (≠ G98), R98 (≠ A101), N126 (≠ D131), K222 (≠ R215)
4r5hA Crystal structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide-adenine-dinucleotide-phosphate and 3-carboxy-propenyl- phthalic acid (see paper)
33% identity, 97% coverage: 4:331/337 of query aligns to 1:340/359 of 4r5hA
- binding 3-[(1E)-3-carboxyprop-1-en-1-yl]benzene-1,2-dicarboxylic acid: S73 (≠ K76), T94 (≠ S97), S95 (≠ G98), R98 (≠ A101), N126 (≠ D131), C127 (≠ S132), Q154 (≠ I159), G158 (≠ A163), K222 (≠ R215), R244 (≠ Q237), H251 (= H244)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), V13 (= V16), A35 (= A38), S36 (≠ R39), R38 (≠ E41), S39 (= S42), T56 (≠ A59), S70 (≠ V73), A71 (= A74), G72 (= G75), T75 (≠ A78), N93 (≠ S96), T94 (≠ S97), P125 (= P130), N126 (≠ D131), C127 (≠ S132), G160 (= G165), M161 (≠ K166), G328 (= G319)
4r4jA Crystal structure of complex sp_asadh with 3-carboxypropyl-phthalic acid and nicotinamide adenine dinucleotide phosphate (see paper)
33% identity, 97% coverage: 4:331/337 of query aligns to 1:340/359 of 4r4jA
- binding 3-(3-carboxypropyl)benzene-1,2-dicarboxylic acid: T94 (≠ S97), S95 (≠ G98), R98 (≠ A101), N126 (≠ D131), C127 (≠ S132), Q154 (≠ I159), G158 (≠ A163), E219 (= E212), K222 (≠ R215), R244 (≠ Q237), H251 (= H244)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), V13 (= V16), A35 (= A38), S36 (≠ R39), R38 (≠ E41), S39 (= S42), T56 (≠ A59), S70 (≠ V73), A71 (= A74), G72 (= G75), T75 (≠ A78), N93 (≠ S96), T94 (≠ S97), N126 (≠ D131), C127 (≠ S132), G160 (= G165), M161 (≠ K166), G328 (= G319)
3pyxB Crystals structure of aspartate beta-semialdehyde dehydrogenase complex with NADP and 2-aminoterephthalate (see paper)
33% identity, 97% coverage: 4:331/337 of query aligns to 1:340/359 of 3pyxB
- binding 2-aminobenzene-1,4-dicarboxylic acid: R98 (≠ A101), G158 (≠ A163), E219 (= E212), K222 (≠ R215), R244 (≠ Q237)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), V13 (= V16), A35 (= A38), S36 (≠ R39), R38 (≠ E41), S39 (= S42), T56 (≠ A59), S70 (≠ V73), A71 (= A74), G72 (= G75), T75 (≠ A78), C127 (≠ S132), S157 (= S162), G158 (≠ A163), G160 (= G165), M161 (≠ K166), N324 (= N315), L325 (≠ V316)
3q11A Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with NADP and aspartyl beta- difluorophosphonate (see paper)
33% identity, 97% coverage: 4:331/337 of query aligns to 1:340/358 of 3q11A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A35 (= A38), S36 (≠ R39), R38 (≠ E41), S39 (= S42), T56 (≠ A59), A71 (= A74), T75 (≠ A78), G160 (= G165), M161 (≠ K166), G162 (≠ K167)
- binding 5,5-difluoro-4-oxo-5-phosphono-D-norvaline: R98 (≠ A101), N126 (≠ D131), C127 (≠ S132), Q154 (≠ I159), G158 (≠ A163), E219 (= E212), K222 (≠ R215), R244 (≠ Q237)
4r54A Complex crystal structure of sp-aspartate-semialdehyde-dehydrogenase with 3-carboxy-ethyl-phthalic acid (see paper)
33% identity, 97% coverage: 4:331/337 of query aligns to 1:340/357 of 4r54A
- binding 3-(2-carboxyethyl)benzene-1,2-dicarboxylic acid: G72 (= G75), S73 (≠ K76), T94 (≠ S97), S95 (≠ G98), R98 (≠ A101), K222 (≠ R215)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), V13 (= V16), A35 (= A38), S36 (≠ R39), R38 (≠ E41), S39 (= S42), T56 (≠ A59), S70 (≠ V73), A71 (= A74), G72 (= G75), T75 (≠ A78), N93 (≠ S96), T94 (≠ S97), N126 (≠ D131), C127 (≠ S132), G160 (= G165), G328 (= G319)
4r41A Complex crystal structure of 4-nitro-2-phosphono-benzoic acid with sp- aspartate-semialdehyde dehydrogenase and nicotinamide-dinucleotide (see paper)
33% identity, 97% coverage: 4:331/337 of query aligns to 1:340/357 of 4r41A
- binding 4-nitro-2-phosphonobenzoic acid: S70 (≠ V73), G72 (= G75), S73 (≠ K76), N93 (≠ S96), T94 (≠ S97), S95 (≠ G98), R98 (≠ A101), K222 (≠ R215)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), A35 (= A38), S36 (≠ R39), R38 (≠ E41), S39 (= S42), T56 (≠ A59), A71 (= A74), G160 (= G165), M161 (≠ K166), G162 (≠ K167)
4r3nA Crystal structure of the ternary complex of sp-asadh with NADP and 1, 2,3-benzenetricarboxylic acid (see paper)
33% identity, 97% coverage: 4:331/337 of query aligns to 1:340/357 of 4r3nA
- active site: C127 (≠ S132), Q154 (≠ I159), R244 (≠ Q237), H251 (= H244)
- binding benzene-1,2,3-tricarboxylic acid: S73 (≠ K76), T94 (≠ S97), S95 (≠ G98), R98 (≠ A101), N126 (≠ D131), K222 (≠ R215)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), V13 (= V16), A35 (= A38), S36 (≠ R39), S39 (= S42), T56 (≠ A59), S70 (≠ V73), A71 (= A74), G72 (= G75), N93 (≠ S96), T94 (≠ S97), N126 (≠ D131), C127 (≠ S132), G160 (= G165), M161 (≠ K166), G328 (= G319)
3q1lA Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with cysteamine bound covalently to cys 128 (see paper)
33% identity, 97% coverage: 4:331/337 of query aligns to 1:340/357 of 3q1lA
3pwsA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
33% identity, 97% coverage: 4:331/337 of query aligns to 1:340/357 of 3pwsA
- binding (2R)-2-aminohexanedioic acid: R98 (≠ A101), N126 (≠ D131), G158 (≠ A163), I208 (vs. gap), E219 (= E212), K222 (≠ R215), R244 (≠ Q237)
- binding adenosine-2'-5'-diphosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), A35 (= A38), S36 (≠ R39), R38 (≠ E41), S39 (= S42), T56 (≠ A59), A71 (= A74), T75 (≠ A78), G160 (= G165), M161 (≠ K166)
3pwkA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
33% identity, 97% coverage: 4:331/337 of query aligns to 1:340/357 of 3pwkA
- binding 5'-o-monophosphoryladenylyl(2'->5')adenylyl(2'->5')adenosine: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), A35 (= A38), S36 (≠ R39), R38 (≠ E41), S39 (= S42), T56 (≠ A59), A71 (= A74), T75 (≠ A78), G160 (= G165)
- binding trans-cyclohexane-1,4-dicarboxylic acid: R98 (≠ A101), N126 (≠ D131), G158 (≠ A163), A159 (≠ Q164), E219 (= E212), K222 (≠ R215), R244 (≠ Q237)
2gz3A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP and aspartate- semialdehyde (see paper)
33% identity, 97% coverage: 4:331/337 of query aligns to 1:340/357 of 2gz3A
- active site: C127 (≠ S132), Q154 (≠ I159), R244 (≠ Q237), H251 (= H244)
- binding (2r)-2-amino-4-oxobutanoic acid: C127 (≠ S132), Q154 (≠ I159), G158 (≠ A163), E219 (= E212), R244 (≠ Q237), H251 (= H244)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), V13 (= V16), A35 (= A38), S36 (≠ R39), R38 (≠ E41), S39 (= S42), T56 (≠ A59), S70 (≠ V73), A71 (= A74), G72 (= G75), T75 (≠ A78), N93 (≠ S96), G158 (≠ A163), G160 (= G165), M161 (≠ K166), N324 (= N315), A329 (≠ G320)
2gz2A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with 2',5'-adp (see paper)
33% identity, 97% coverage: 4:331/337 of query aligns to 1:340/357 of 2gz2A
- active site: C127 (≠ S132), Q154 (≠ I159), R244 (≠ Q237), H251 (= H244)
- binding adenosine-2'-5'-diphosphate: G8 (= G11), T10 (= T13), G11 (= G14), A35 (= A38), S36 (≠ R39), R38 (≠ E41), S39 (= S42), T56 (≠ A59), A71 (= A74), T75 (≠ A78)
2gz1A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP (see paper)
33% identity, 97% coverage: 4:331/337 of query aligns to 1:340/357 of 2gz1A
- active site: C127 (≠ S132), Q154 (≠ I159), R244 (≠ Q237), H251 (= H244)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), V13 (= V16), A35 (= A38), S36 (≠ R39), R38 (≠ E41), S39 (= S42), T56 (≠ A59), S70 (≠ V73), A71 (= A74), G72 (= G75), T75 (≠ A78), N93 (≠ S96), S157 (= S162), G158 (≠ A163), G160 (= G165), M161 (≠ K166), N324 (= N315), L325 (≠ V316)
3tz6A Crystal structure of aspartate semialdehyde dehydrogenase complexed with inhibitor smcs (cys) and phosphate from mycobacterium tuberculosis h37rv (see paper)
33% identity, 97% coverage: 4:331/337 of query aligns to 1:340/342 of 3tz6A
- active site: C129 (≠ S132), Q156 (≠ I159), R248 (≠ Q237), H255 (= H244)
- binding cysteine: C129 (≠ S132), Q156 (≠ I159), G160 (≠ A163), E223 (= E212), R248 (≠ Q237), H255 (= H244)
- binding glycerol: S108 (≠ P111), G187 (vs. gap), F192 (≠ I185), P201 (≠ Q194), Q225 (≠ R214), R228 (≠ V217), F229 (≠ D218), Q335 (≠ K326), E338 (= E329), L339 (≠ K330)
- binding sulfate ion: R98 (≠ A101), H117 (≠ D120), R119 (= R122), N128 (≠ D131), C129 (≠ S132), K226 (≠ R215), E270 (= E259), R273 (= R262)
Q57658 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
24% identity, 100% coverage: 1:336/337 of query aligns to 1:354/354 of Q57658
Query Sequence
>16426 FitnessBrowser__Keio:16426
MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAGEQLRFGGKTITVQDAA
EFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFALEPDVPLVVPEVNPFVLTD
YRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLISASAQGKKAVDALAGQSAKLL
NGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVDEVRKILQDEGLMISASVVQAPV
FYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEENEFPTQVGDASGTPHLSVGCVRND
YGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQEYLY
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory