SitesBLAST

C9 (= C11) mutation to A: Appears to be unable to inactivate ManR, since ManR does not show any decrease in its DNA-binding activity in the presence of this mutant.

Sites not aligning to the query:

365 modified: Phosphoserine

P20966 PTS system fructose-specific EIIB'BC component; EIIB'BC-Fru; EC 2.7.1.202 from Escherichia coli (strain K12) (see paper)
38% identity, 94% coverage: 3:104/108 of query aligns to 104:201/563 of P20966

query
sites
P20966

K

K

K

R

L

V

I

V

A

A

L

V

C

T

A

A

C
|
C

P

P

M

T

G

G

L

V

A

A

H

H

T

T

F

F

M

M

A

A

Q

E

A

A

L

I

E

E

E

T

A

E

A

A

V

K

E

K

A

R

G

G

Y

W

E

W

V

V

K

K

I

V

E

E

T

T

Q

R

G

G

A

S

D

V

G

G

I

A

Q

G

N

N

R

A

L

I

T

T

A

P

Q

E

D

E

I

V

A

A

E

A

A

A

T

D

I

L

I

V

I

I

H

-

S

-

V

V

A

A

V

A

T

D

P

I

E

E

-

V

D

D

N

L

E

A

R

K

F

F

E

A

S

G

R

K

D

P

V

M

Y

Y

E

R

I

T

T

S

L

T

Q

G

D

L

A

A

I

L

K

K

N

K

A

T

A

A

G

-

I

-

K

Q

E

E

I

L

E

D

E

K

M

A

I

V

A

A

C112 (= C11) active site, Phosphocysteine intermediate; for EIIB activity

Query Sequence

>16492 FitnessBrowser__Keio:16492
MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGADGIQNRLTAQDIAEATI
IIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEMIASEQQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory