SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 16516 b2417 glucose-specific PTS system enzyme IIA component (NCBI) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

P69783 PTS system glucose-specific EIIA component; EIIA-Glc; EIII-Glc; Glucose-specific phosphotransferase enzyme IIA component from Escherichia coli (strain K12) (see 6 papers)
100% identity, 100% coverage: 1:169/169 of query aligns to 1:169/169 of P69783

query
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P69783

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M1 (= M1) modified: Initiator methionine, Removed
F4 (= F4) mutation to W: Same activity as the wild-type.
H76 (= H76) Important for phospho-donor activity; binding ; mutation to Q: Unable to transfer phosphoryl group.
H91 (= H91) active site, Tele-phosphohistidine intermediate; for EIIA activity; binding ; modified: Phosphohistidine; by HPr; mutation to Q: Unable to be phosphorylated by Hpr.

1glcF Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
96% identity, 99% coverage: 2:169/169 of query aligns to 1:161/161 of 1glcF

query
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1glcF

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binding zinc ion: H68 (= H76), H83 (= H91)

1o2fA Complex of enzyme iiaglc and iibglc phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
100% identity, 89% coverage: 20:169/169 of query aligns to 1:150/150 of 1o2fA

query
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1o2fA

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active site: T55 (= T74), H57 (= H76), H72 (= H91)
binding phosphite ion: H72 (= H91), D76 (= D95)

P09323 PTS system N-acetylglucosamine-specific EIICBA component; EIICBA-Nag; EII-Nag; EC 2.7.1.193 from Escherichia coli (strain K12) (see paper)
50% identity, 88% coverage: 22:169/169 of query aligns to 500:648/648 of P09323

query
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P09323

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Sites not aligning to the query:

1 modified: N-formylmethionine

Query Sequence

>16516 b2417 glucose-specific PTS system enzyme IIA component (NCBI)
MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAEKIVGDGIAIKPTGNKM
VAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGEGFKRIAEEGQRVKVGDTV
IEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVGETPVIRIKK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory