SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 16520 FitnessBrowser__Keio:16520 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P16703 Cysteine synthase B; CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B; EC 2.5.1.47 from Escherichia coli (strain K12) (see paper)
100% identity, 100% coverage: 1:303/303 of query aligns to 1:303/303 of P16703

query
sites
P16703

M

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I

N71 (= N71) binding
S255 (= S255) binding

2bhtA Crystal structure of o-acetylserine sulfhydrylase b (see paper)
99% identity, 97% coverage: 1:294/303 of query aligns to 1:294/294 of 2bhtA

query
sites
2bhtA

M

M

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S

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I

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active site: K41 (= K41), S69 (= S69), Q199 (= Q199), G203 (= G203), S255 (= S255), C280 (= C280)
binding pyridoxal-5'-phosphate: K41 (= K41), N71 (= N71), M173 (= M173), G174 (= G174), T175 (= T175), T176 (= T176), T178 (= T178), G208 (= G208), S255 (= S255), C280 (= C280)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
44% identity, 95% coverage: 6:292/303 of query aligns to 10:306/310 of 5xoqA

query
sites
5xoqA

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F

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binding : T72 (= T68), S73 (= S69), G74 (= G70), T76 (= T72), M123 (= M119), Q144 (= Q140), R218 (vs. gap), H219 (vs. gap), Q222 (≠ R210), G223 (≠ R211), A226 (≠ T214)

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
45% identity, 95% coverage: 6:292/303 of query aligns to 9:306/320 of 2isqA

query
sites
2isqA

Q

E

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x
K

G

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x
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K

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x
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active site: K44 (= K41), S267 (= S255)
binding pyridoxal-5'-phosphate: K44 (= K41), N75 (= N71), G177 (≠ S172), G179 (= G174), T180 (= T175), G181 (≠ T176), T183 (= T178), G223 (vs. gap), S267 (= S255), P294 (≠ C280)
binding : T72 (= T68), S73 (= S69), G74 (= G70), T76 (= T72), G122 (= G118), M123 (= M119), K124 (≠ E120), G217 (≠ W212), P218 (= P213), H219 (≠ T214), Q222 (vs. gap), G223 (vs. gap)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
45% identity, 95% coverage: 6:292/303 of query aligns to 11:308/322 of P47998

query
sites
P47998

Q

E

T

L

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I

G

G

N

N

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T

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P

L

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K

Y

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K

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K
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K

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R

A

I

A

G

L

F

S

S

M

M

I

I

V

S

E

D

A

A

E

E

K

K

R

K

G

G

E

L

I

I

K

K

P

P

G

G

D

E

-

S

V

V

L

L

I

I

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

I

T

A

A

L

A

K

K

G

G

Y

Y

R

K

M

L

K

I

L

I

L

T

M

M

P

P

D

A

N

S

M

M

S

S

Q

T

E

E

R

R

A

I

M

L

R

L

A

A

Y

F

G

G

A

V

E

E

L

L

I

V

L

L

V

T

T

D

K

P

E

A

Q

K

G

G

M

M

E

K

G

G

A

A

R

I

D

A

L

K

A

A

L

E

E

E

-

I

M

L

A

A

N

K

R

T

G

P

E

N

G

G

K

Y

L

M

L

L

D

Q

Q
|
Q

F

F

N

E

N

N

P

P

D

A

N

N

P

P

Y

K

A

I

H
|
H

Y

Y

T

E

T

T

G
|
G

P

P

E

E

I

I

W

W

Q

K

Q

G

T

T

G

G

R

K

I

I

T

D

H

G

F

F

V

V

S

S

S

G

M

I

G
|
G

T
|
T

T
x
G

G
|
G

T
|
T

I

I

T

T

G

G

V

A

S

G

R

K

F

Y

M

L

R

K

E

E

Q

Q

S

N

K

A

P

N

V

V

T

K

I

L

V

Y

G

G

L

V

Q

E

P

P

E

V

E

E

G

S

S

A

S

I

I

L

P

S

G

G

I

G

R
x
K

R

P

W

G

P

P

T
x
H

E
x
K

-

I

-

Q

-

G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

G

S

I

V

F

L

N

N

A

V

S

D

L

L

V

I

D

D

E

E

V

V

L

V

D

Q

I

V

H

S

Q

S

R

D

D

E

A

S

E

I

N

D

T

M

M

A

R

R

E

Q

L

L

A

A

V

L

R

K

E

E

G

G

I

L

F

L

C

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

V

A

A

A

G

A

A

A

L

I

R

K

V

L

A

A

K

Q

-

R

-

P

A

E

N

N

P

A

D

G

A

K

V

L

V

F

V

V

A

A

I

I

I

F

C

P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

K46 (= K41) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T68) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S69) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N71) binding ; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T72) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q140) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H150) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (= G155) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (≠ GTTGT 174:178) binding
T182 (= T175) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T178) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ R210) mutation to A: Impaired interaction with SAT1.
H221 (≠ T214) mutation to A: Impaired interaction with SAT1.
K222 (≠ E215) mutation to A: Impaired interaction with SAT1.
S269 (= S255) binding ; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
45% identity, 95% coverage: 6:292/303 of query aligns to 9:306/320 of 1z7yA

query
sites
1z7yA

Q

E

T

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

K

Y

L

L

Q

N

R

N

M

V

G

A

P

E

D

G

N

C

G

V

S

G

E

R

V

V

W

A

L

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K
x
A

D

D

R

R

A

I

A

G

L

F

S

S

M

M

I

I

V

S

E

D

A

A

E

E

K

K

R

K

G

G

E

L

I

I

K

K

P

P

G

G

D

E

-

S

V

V

L

L

I

I

E

E

A

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

I

T

A

A

L

A

K

K

G

G

Y

Y

R

K

M

L

K

I

L

I

L

T

M

M

P

P

D

A

N

S

M

M

S

S

Q

T

E

E

R

R

A

I

M

L

R

L

A

A

Y

F

G

G

A

V

E

E

L

L

I

V

L

L

V

T

T

D

K

P

E

A

Q

K

G

G

M

M

E

K

G

G

A

A

R

I

D

A

L

K

A

A

L

E

E

E

-

I

M

L

A

A

N

K

R

T

G

P

E

N

G

G

K

Y

L

M

L

L

D

Q

Q
|
Q

F

F

N

E

N

N

P

P

D

A

N

N

P

P

Y

K

A

I

H

H

Y

Y

T

E

T

T

G

G

P

P

E

E

I

I

W

W

Q

K

Q

G

T

T

G

G

R

K

I

I

T

D

H

G

F

F

V

V

S

S

S

G

M
x
I

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

T

T

G

G

V

A

S

G

R

K

F

Y

M

L

R

K

E

E

Q

Q

S

N

K

A

P

N

V

V

T

K

I

L

V

Y

G

G

L

V

Q

E

P

P

E

V

E

E

G

S

S

A

S

I

I

L

P

S

G

G

I

G

R

K

R

P

W

G

P

P

T

H

E

K

-

I

-

Q

-
x
G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

G

S

I

V

F

L

N

N

A

V

S

D

L

L

V

I

D

D

E

E

V

V

L

V

D

Q

I

V

H

S

Q

S

R

D

D

E

A

S

E

I

N

D

T

M

M

A

R

R

E

Q

L

L

A

A

V

L

R

K

E

E

G

G

I

L

F

L

C

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

V

A

A

A

G

A

A

A

L

I

R

K

V

L

A

A

K

Q

-

R

-

P

A

E

N

N

P

A

D

G

A

K

V

L

V

F

V

V

A

A

I

I

I

F

C
x
P

D
x
S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

active site: A44 (≠ K41), S267 (= S255)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G70), N75 (= N71), T76 (= T72), Q145 (= Q140), I178 (≠ M173), G179 (= G174), T180 (= T175), G181 (≠ T176), T183 (= T178), G223 (vs. gap), S267 (= S255), P294 (≠ C280), S295 (≠ D281)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
45% identity, 95% coverage: 6:292/303 of query aligns to 9:306/309 of 7n2tA

query
sites
7n2tA

Q

E

T

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

K

Y

L

L

Q

N

R

R

M

V

G

V

P

D

D

G

N

C

G

V

S

A

E

R

V

V

W

A

L

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K

K

D

D

R

R

A

I

A

G

L

Y

S

S

M

M

I

I

V

S

E

D

A

A

E

E

K

N

R

K

G

G

E

L

I

I

K

T

P

P

G

G

D

E

-

S

V

V

L

L

I

I

E

E

A

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

I

I

A

A

L

A

K

K

G

G

Y

Y

R

R

M

L

K

I

L

I

L

C

M

M

P

P

D

A

N

S

M

M

S

S

Q

L

E

E

R

R

A

T

A

I

M

L

R

R

A

A

Y

F

G

G

A

A

E

E

L

L

I

V

L

L

V

T

T

D

K

P

E

A

Q

R

G

G

M

M

E

K

G

G

A

A

R

V

D

Q

L

K

A

A

L

E

E

E

M

I

-

K

A

A

N

K

R

T

G

P

E

N

G

S

K

Y

L

I

L

L

D

Q

Q

Q

F

F

N

E

N

N

P

P

D

A

N

N

P

P

Y

K

A

I

H

H

Y

Y

T

E

T

T

G

G

P

P

E

E

I

I

W

W

Q

R

Q

G

T

S

G

G

R

K

I

I

T

D

H

A

F

L

V

V

S

S

S

G

M

I

G

G

T

T

T

G

G

G

T

T

I

V

T

T

G

G

V

A

S

G

R

K

F

Y

M

L

R

K

E

E

Q

Q

S

N

K

P

P

N

V

I

T

K

I

L

V

Y

G

G

L

V

Q

E

P

P

E

V

E

E

G

S

S

A

S

I

I

L

P

S

G

G

I

G

R

K

R

P

W

G

P

P

T

H

E

K

-

I

-

Q

-

G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

G

G

I

V

F

L

N

D

A

V

S

N

L

L

V

L

D

D

E

E

V

V

L

I

D

Q

I

V

H

S

Q

S

R

E

D

E

A

S

E

I

N

E

T

T

M

A

R

K

E

L

L

L

A

A

V

L

R

K

E

E

G

G

I

L

F

L

C

V

G

G

V

I

S

S

G

G

G

A

A

A

V

A

A

A

G

A

A

A

L

I

R
|
R

V

I

A

A

K

K

-
x
R

-

P

A

E

N

N

P

A

D

G

A

K

V

L

V

I

V

V

A

A

I

V

I

F

C

P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

binding phosphate ion: R277 (= R265), R281 (vs. gap)

2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
44% identity, 95% coverage: 4:292/303 of query aligns to 3:301/302 of 2efyA

query
sites
2efyA

L

V

E

E

Q

G

T

A

I

I

G

G

N

K

T

T

P

P

L

V

V

V

K

R

L

L

Q

A

R

K

M

V

G

V

P

E

D

P

N

D

G

M

S

A

E

E

V

V

W

W

L

V

K

K

L

L

E

E

G

G

N

L

N

N

P

P

A

G

G

G

S

S

V

I

K
|
K

D

D

R

R

A

P

A

A

L

W

S

Y

M

M

I

I

V

K

E

D

A

A

E

E

K

E

R

R

G

G

E

I

I

L

K

R

P

P

G

G

D

S

-

G

-

Q

V

V

L

I

I

V

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

I

I

A

A

L

S

K

R

G

G

Y

Y

R

R

M

L

K

I

L

L

L

T

M

M

P

P

D

A

N

Q

M

M

S

S

Q

E

E

E

R

R

R

K

A

R

A

V

M

L

R

K

A

A

Y

F

G

G

A

A

E

E

L

L

I

V

L

L

V

T

T

D

K

P

E

E

Q

R

G

R

M

M

E

L

G

A

A

A

R

R

D

E

L

E

A

A

L

L

E

R

M

L

A

K

N

E

R

E

G

L

E

G

G

A

K

F

L

M

L

P

D

D

Q
|
Q

F

F

N

K

N

N

P

P

D

A

N

N

P

V

Y

R

A

A

H

H

Y

Y

T

E

T

T

G

G

P

P

E

E

I

L

W

Y

Q

E

Q

A

T

L

G

E

G

G

R

R

I

I

T

D

H

A

F

F

V

V

S
x
Y

S

G

M

S

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

T

T

G

G

V

V

S

G

R

R

F

Y

M

L

R

K

E

E

Q

R

S

I

K

P

P

H

V

V

T

K

I

V

V

I

G

A

L

V

Q
x
E

P

P

E

A

E

R

G
x
S

S

N

S

V

I

L

P

S

-

G

-

K

-

M

-

G

-

Q

-

H

G

G

I

F

R

Q

R
x
G

W
x
M

P

G

T
x
P

E

G

Y

F

L

I

P

P

G

E

I

N

F

L

N

D

A

L

S

S

L

L

V

L

D

D

E

G

V

V

L

I

D

Q

I

V

H

W

Q

E

R

E

D

D

A

A

E

F

N

P

T

L

M

A

R

R

E

R

L

L

A

A

V

R

R

E

E

E

G

G

I

L

F

F

C

L

G

G

V

M

S
|
S

S

S

G

G

A

I

V

V

A

W

G

A

A

A

L

L

R

Q

V

V

A

A

K

R

A

E

-

L

N

G

P

P

D

G

A

K

V

R

V

V

V

A

A

C

I

I

I

S

C
x
P

D
|
D

R

G

G

G

D

W

R

K

Y

Y

L

L

S

S

T

T

G

P

V

L

F

Y

active site: K40 (= K41), S70 (= S69), E200 (≠ Q199), S204 (≠ G203), S263 (= S255)
binding 5-oxohexanoic acid: T69 (= T68), G71 (= G70), T73 (= T72), Q141 (= Q140), G175 (= G174), G219 (≠ R211), M220 (≠ W212), P222 (≠ T214)
binding pyridoxal-5'-phosphate: K40 (= K41), N72 (= N71), Y172 (≠ S171), G175 (= G174), T176 (= T175), G177 (≠ T176), T179 (= T178), G219 (≠ R211), S263 (= S255), P289 (≠ C280), D290 (= D281)

2ecqA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 3-hydroxylactate
44% identity, 95% coverage: 4:292/303 of query aligns to 3:301/302 of 2ecqA

query
sites
2ecqA

L

V

E

E

Q

G

T

A

I

I

G

G

N

K

T

T

P

P

L

V

V

V

K

R

L

L

Q

A

R

K

M

V

G

V

P

E

D

P

N

D

G

M

S

A

E

E

V

V

W

W

L

V

K

K

L

L

E

E

G

G

N

L

N

N

P

P

A

G

G

G

S

S

V

I

K
|
K

D

D

R

R

A

P

A

A

L

W

S

Y

M

M

I

I

V

K

E

D

A

A

E

E

K

E

R

R

G

G

E

I

I

L

K

R

P

P

G

G

D

S

-

G

-

Q

V

V

L

I

I

V

E

E

A

P

T

T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

I

I

A

A

L

S

K

R

G

G

Y

Y

R

R

M

L

K

I

L

L

L

T

M

M

P

P

D

A

N

Q

M

M

S

S

Q

E

E

E

R

R

R

K

A

R

A

V

M

L

R

K

A

A

Y

F

G

G

A

A

E

E

L

L

I

V

L

L

V

T

T

D

K

P

E

E

Q

R

G

R

M

M

E

L

G

A

A

A

R

R

D

E

L

E

A

A

L

L

E

R

M

L

A

K

N

E

R

E

G

L

E

G

G

A

K

F

L

M

L

P

D

D

Q
|
Q

F

F

N

K

N

N

P

P

D

A

N

N

P

V

Y

R

A

A

H

H

Y

Y

T

E

T

T

G

G

P

P

E

E

I

L

W

Y

Q

E

Q

A

T

L

G

E

G

G

R

R

I

I

T

D

H

A

F

F

V

V

S
x
Y

S
x
G

M

S

G
|
G

T
|
T

T

G

G

G

T
|
T

I

I

T

T

G

G

V

V

S

G

R

R

F

Y

M

L

R

K

E

E

Q

R

S

I

K

P

P

H

V

V

T

K

I

V

V

I

G

A

L

V

Q
x
E

P

P

E

A

E

R

G
x
S

S

N

S

V

I

L

P

S

-

G

-

K

-

M

-

G

-

Q

-

H

G

G

I

F

R

Q

R
x
G

W

M

P

G

T

P

E

G

Y

F

L

I

P

P

G

E

I

N

F

L

N

D

A

L

S

S

L

L

V

L

D

D

E

G

V

V

L

I

D

Q

I

V

H

W

Q

E

R

E

D

D

A

A

E

F

N

P

T

L

M

A

R

R

E

R

L

L

A

A

V

R

R

E

E

E

G

G

I

L

F

F

C

L

G

G

V

M

S
|
S

S

S

G

G

A

I

V

V

A

W

G

A

A

A

L

L

R

Q

V

V

A

A

K

R

A

E

-

L

N

G

P

P

D

G

A

K

V

R

V

V

V

A

A

C

I

I

I

S

C
x
P

D

D

R

G

G

G

D

W

R

K

Y

Y

L

L

S

S

T

T

G

P

V

L

F

Y

active site: K40 (= K41), S70 (= S69), E200 (≠ Q199), S204 (≠ G203), S263 (= S255)
binding (3s)-3-hydroxybutanoic acid: K40 (= K41), G71 (= G70), T73 (= T72), Q141 (= Q140), G219 (≠ R211)
binding pyridoxal-5'-phosphate: K40 (= K41), N72 (= N71), Y172 (≠ S171), G173 (≠ S172), G175 (= G174), T176 (= T175), T179 (= T178), G219 (≠ R211), S263 (= S255), P289 (≠ C280)

2ecoA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-methylvalerate
44% identity, 95% coverage: 4:292/303 of query aligns to 3:301/302 of 2ecoA

query
sites
2ecoA

L

V

E

E

Q

G

T

A

I

I

G

G

N

K

T

T

P

P

L

V

V

V

K

R

L

L

Q

A

R

K

M

V

G

V

P

E

D

P

N

D

G

M

S

A

E

E

V

V

W

W

L

V

K

K

L

L

E

E

G

G

N

L

N

N

P

P

A

G

G

G

S

S

V

I

K
|
K

D

D

R

R

A

P

A

A

L

W

S

Y

M

M

I

I

V

K

E

D

A

A

E

E

K

E

R

R

G

G

E

I

I

L

K

R

P

P

G

G

D

S

-

G

-

Q

V

V

L

I

I

V

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

I

I

A

A

L

S

K

R

G

G

Y

Y

R

R

M

L

K

I

L

L

L

T

M

M

P

P

D

A

N

Q

M

M

S

S

Q

E

E

E

R

R

R

K

A

R

A

V

M

L

R

K

A

A

Y

F

G

G

A

A

E

E

L

L

I

V

L

L

V

T

T

D

K

P

E

E

Q

R

G

R

M

M

E

L

G

A

A

A

R

R

D

E

L

E

A

A

L

L

E

R

M

L

A

K

N

E

R

E

G

L

E

G

G

A

K

F

L

M

L

P

D

D

Q
|
Q

F

F

N

K

N

N

P

P

D

A

N

N

P

V

Y

R

A

A

H

H

Y

Y

T

E

T

T

G

G

P

P

E

E

I

L

W

Y

Q

E

Q

A

T

L

G

E

G

G

R

R

I

I

T

D

H

A

F

F

V

V

S
x
Y

S

G

M

S

G
|
G

T
|
T

T

G

G

G

T
|
T

I

I

T

T

G

G

V

V

S

G

R

R

F

Y

M

L

R

K

E

E

Q

R

S

I

K

P

P

H

V

V

T

K

I

V

V

I

G

A

L

V

Q
x
E

P

P

E

A

E

R

G
x
S

S

N

S

V

I

L

P

S

-

G

-

K

-

M

-

G

-

Q

-

H

G

G

I

F

R

Q

R
x
G

W

M

P

G

T

P

E

G

Y

F

L

I

P

P

G

E

I

N

F

L

N

D

A

L

S

S

L

L

V

L

D

D

E

G

V

V

L

I

D

Q

I

V

H

W

Q

E

R

E

D

D

A

A

E

F

N

P

T

L

M

A

R

R

E

R

L

L

A

A

V

R

R

E

E

E

G

G

I

L

F

F

C

L

G

G

V

M

S
|
S

S

S

G

G

A

I

V

V

A

W

G

A

A

A

L

L

R

Q

V

V

A

A

K

R

A

E

-

L

N

G

P

P

D

G

A

K

V

R

V

V

V

A

A

C

I

I

I

S

C
x
P

D
|
D

R

G

G

G

D

W

R

K

Y

Y

L

L

S

S

T

T

G

P

V

L

F

Y

active site: K40 (= K41), S70 (= S69), E200 (≠ Q199), S204 (≠ G203), S263 (= S255)
binding 4-methyl valeric acid: K40 (= K41), T69 (= T68), G71 (= G70), T73 (= T72), Q141 (= Q140), G175 (= G174), T176 (= T175), G219 (≠ R211)
binding pyridoxal-5'-phosphate: K40 (= K41), N72 (= N71), Y172 (≠ S171), G175 (= G174), T176 (= T175), T179 (= T178), G219 (≠ R211), S263 (= S255), P289 (≠ C280), D290 (= D281)

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
43% identity, 95% coverage: 4:292/303 of query aligns to 17:316/323 of 4aecA

query
sites
4aecA

L

V

E

S

Q

Q

T

L

I

I

G

G

N

K

T

T

P

P

L

M

V

V

K

Y

L

L

Q

N

R

S

M

I

G

A

P

K

D

G

N

C

G

V

S

A

E

N

V

I

W

A

L

A

K

K

L

L

E

E

G

I

N

M

N

E

P

P

A

C

G

C

S

S

V

V

K
|
K

D

D

R

R

A

I

A

G

L

Y

S

S

M

M

I

V

V

T

E

D

A

A

E

E

K

Q

R

K

G

G

E

F

I

I

K

S

P

P

G

G

-

K

D

S

V

V

L

L

I

V

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

I

I

A

A

L

S

K

R

G

G

Y

Y

R

R

M

L

K

I

L

L

L

T

M

M

P

P

D

A

N

S

M

M

S

S

Q

M

E

E

R

R

A

V

A

L

M

L

R

K

A

A

Y

F

G

G

A

A

E

E

L

L

I

V

L

L

V

T

T

D

K

P

E

A

Q

K

G

G

M

M

E

T

G

G

A

A

R

V

D

Q

L

K

A

A

L

E

E

E

-

I

M

L

A

K

N

N

R

T

G

P

E

D

G

A

K

Y

L

M

L

L

D

Q

Q

Q

F

F

N

D

N

N

P

P

D

A

N

N

P

P

Y

K

A

I

H

H

Y

Y

T

E

T

T

G

G

P

P

E

E

I

I

W

W

Q

D

T

T

G

K

G

G

R

K

I

V

T

D

H

I

F

F

V

V

S

A

S

G

M
x
I

G
|
G

T
|
T

T
x
G

G
|
G

T
|
T

I

I

T

T

G

G

V

V

S

G

R

R

F

F

M

I

R

K

E

E

Q

K

S

N

K

P

P

K

V

T

T

Q

I

V

V

I

G

G

L

V

Q

E

P

P

E

T

E

E

G

S

S

D

S

I

I

L

P

S

G

G

I

G

R

K

R

P

W

G

P

P

T

H

E

K

-

I

-

Q

-
x
G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

G

K

I

N

F

L

N

D

A

Q

S

K

L

I

V

M

D

D

E

E

V

V

L

I

D

A

I

I

H

S

Q

S

R

E

D

E

A

A

E

I

N

E

T

T

M

A

R

K

E

Q

L

L

A

A

V

L

R

K

E

E

G

G

I

L

F

M

C

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

V

A

A

A

G

A

A

A

L

I

R

K

V

V

A

A

K

K

-

R

-

P

A

E

N

N

P

A

D

G

A

K

V

L

V

I

V

A

A

V

I

V

I

F

C
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

P

V

L

F

F

active site: K54 (= K41), S277 (= S255)
binding pyridoxal-5'-phosphate: K54 (= K41), N85 (= N71), I188 (≠ M173), G189 (= G174), T190 (= T175), G191 (≠ T176), G192 (= G177), T193 (= T178), G233 (vs. gap), S277 (= S255), P304 (≠ C280)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
42% identity, 96% coverage: 4:294/303 of query aligns to 7:307/310 of P9WP55

query
sites
P9WP55

L

I

E

T

Q

Q

T

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

K

R

L

L

Q

R

R

R

M

V

G

T

P

D

D

G

N

A

G

V

S

A

E

D

V

I

W

V

L

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

A

I

A

G

L

V

S

A

M

M

I

L

V

Q

E

A

A

A

E

E

K

Q

R

A

G

G

E

L

I

I

K

K

P

P

G

D

D

T

V

I

L

I

I

L

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

M

I

V

A

C

A

A

L

A

K

R

G

G

Y

Y

R

R

M

C

K

V

L

L

L

T

M

M

P

P

D

E

N

T

M

M

S

S

Q

L

E

E

R

R

A

M

A

L

M

L

R

R

A

A

Y

Y

G

G

A

A

E

E

L

L

I

I

L

L

V

T

T

P

K

G

E

A

Q

D

G

G

M

M

E

S

G

G

A

A

R

I

D

A

L

K

A

A

L

E

E

E

M

L

A

A

N

K

R

T

G

D

E

Q

G

R

K

Y

L

F

L

V

-

P

D

Q

Q

Q

F

F

N

E

N

N

P

P

D

A

N

N

P

P

Y

A

A

I

H

H

Y

R

T

V

T

T

G

A

P

E

E

E

I

V

W

W

Q

R

Q

D

T

T

G

D

G

G

R

K

I

V

T

D

H

I

F

V

V

V

S

A

S

G

M

V

G
|
G

T
|
T

T
x
G

G
|
G

T
|
T

I

I

T

T

G

G

V

V

S

A

R

Q

F

V

M

I

R

K

E

E

Q

R

S

K

K

P

P

S

V

A

T

R

I

F

V

V

G

A

L

V

Q

E

P

P

E

A

G

S

S

P

S

V

I

L

P

S

G

G

I

G

R

Q

R

K

W

G

P

P

T

H

E

P

-

I

-

Q

-

G

-

I

-

G

-

A

-

G

Y

F

L

V

P

P

G

P

I

V

F

L

N

D

A

Q

S

D

L

L

V

V

D

D

E

E

V

I

L

I

D

T

I

V

H

G

Q

N

R

E

D

D

A

A

E

L

N

N

T

V

M

A

R

R

E

R

L

L

A

A

V

R

R

E

E

E

G

G

I

L

F

L

C

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

V

T

A

V

G

A

A

A

L

L

R

Q

V

V

A

A

K

R

-

R

-

P

A

E

N

N

P

A

D

G

A

K

V

L

V

I

V

V

A

V

I

V

I

L

C

P

D

D

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

P

V

L

F

F

G

A

E

D

K44 (= K41) modified: N6-(pyridoxal phosphate)lysine
N74 (= N71) binding
GTGGT 178:182 (≠ GTTGT 174:178) binding
S266 (= S255) binding

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
42% identity, 96% coverage: 4:293/303 of query aligns to 7:306/306 of 2q3dA

query
sites
2q3dA

L

I

E

T

Q

Q

T

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

K

R

L

L

Q

R

R

R

M

V

G

T

P

D

D

G

N

A

G

V

S

A

E

D

V

I

W

V

L

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

A

I

A

G

L

V

S

A

M

M

I

L

V

Q

E

A

A

A

E

E

K

Q

R

A

G

G

E

L

I

I

K

K

P

P

G

D

D

T

V

I

L

I

I

L

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

M

I

V

A

C

A

A

L

A

K

R

G

G

Y

Y

R

R

M

C

K

V

L

L

L

T

M

M

P

P

D

E

N

T

M

M

S

S

Q

L

E

E

R

R

A

M

A

L

M

L

R

R

A

A

Y

Y

G

G

A

A

E

E

L

L

I

I

L

L

V

T

T

P

K

G

E

A

Q

D

G

G

M

M

E

S

G

G

A

A

R

I

D

A

L

K

A

A

L

E

E

E

M

L

A

A

N

K

R

T

G

D

E

Q

G

R

K

Y

L

F

L

V

-

P

D

Q

Q
|
Q

F

F

N

E

N

N

P

P

D

A

N

N

P

P

Y

A

A

I

H

H

Y

R

T

V

T

T

G

A

P

E

E

E

I

V

W

W

Q

R

Q

D

T

T

G

D

G

G

R

K

I

V

T

D

H

I

F

V

V

V

S

A

S

G

M
x
V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

T

T

G

G

V

V

S

A

R

Q

F

V

M

I

R

K

E

E

Q

R

S

K

K

P

P

S

V

A

T

R

I

F

V

V

G

A

L

V

Q

E

P

P

E

A

G

S

S

P

S

V

I

L

P

S

G

G

I

G

R

Q

R

K

W

G

P

P

T

H

E

P

-

I

-

Q

-
x
G

-
x
I

-

G

-

A

-

G

Y

F

L

V

P

P

G

P

I

V

F

L

N

D

A

Q

S

D

L

L

V

V

D

D

E

E

V

I

L

I

D

T

I

V

H

G

Q

N

R

E

D

D

A

A

E

L

N

N

T

V

M

A

R

R

E

R

L

L

A

A

V

R

R

E

E

E

G

G

I

L

F

L

C

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

V

T

A

V

G

A

A

A

L

L

R

Q

V

V

A

A

K

R

-

R

-

P

A

E

N

N

P

A

D

G

A

K

V

L

V

I

V

V

A

V

I

V

I

L

C
x
P

D
|
D

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

P

V

L

F

F

G

A

active site: K44 (= K41), S266 (= S255), P293 (≠ C280)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K41), T71 (= T68), S72 (= S69), N74 (= N71), T75 (= T72), Q144 (= Q140), V177 (≠ M173), G178 (= G174), T179 (= T175), G180 (≠ T176), T182 (= T178), G222 (vs. gap), I223 (vs. gap), S266 (= S255), P293 (≠ C280), D294 (= D281)

3x43A Crystal structure of o-ureido-l-serine synthase (see paper)
43% identity, 96% coverage: 1:292/303 of query aligns to 3:302/316 of 3x43A

query
sites
3x43A

M

F

S

N

T

S

L

I

E

L

Q

D

T

T

I

I

G

G

N

R

T

T

P

P

L

I

V

V

K

R

L

L

Q

Q

R

R

M

M

G

A

P

P

D

E

N

H

G

T

S

S

E

-

V

V

W

Y

L

V

K

K

L

V

E

E

G

S

N

F

N

N

P

P

A

G

G

G

S

S

V

V

K
|
K

D

D

R

R

A

L

A

A

L

L

S

S

M

V

I

V

V

L

E

D

A

A

E

E

K

A

R

K

G

G

E

L

I

L

K

K

P

P

G

G

D

D

V

T

L

I

I

V

E

E

A

C

T

T

S

S

G

G

N
|
N

T

V

G

G

I

I

A

A

L

L

A

A

M

M

I

V

A

A

L

A

K

R

G

G

Y

Y

R

R

M

F

K

V

L

A

L

V

M

M

P

G

D

D

N

T

M

Y

S

S

Q

V

E

E

R

R

A

K

A

L

M

I

R

R

A

A

Y

Y

G

G

A

G

E

K

L

L

I

V

L

L

V

F

T

P

K

G

E

H

Q

L

G

G

M

S

E

K

G

G

A

G

R

N

D

L

L

I

A

A

L

D

E

E

M

L

A

A

N

E

R

K

G

Y

E

G

G

W

K

F

L

R

L

A

D

R

Q

Q

F

F

N

D

N

N

P

P

D

A

N

N

P

P

Y

S

A

Y

H

H

Y

R

T

E

T

T

G

A

P

S

E

E

I

I

W

L

Q

A

Q

D

T

F

G

A

G

G

-

K

R

R

I

L

T

D

H

H

F

F

V

V

S

T

S

G

M
x
F

G
|
G

T
|
T

G

G

T
|
T

I

L

T

T

G

G

V

V

S

G

R

Q

F

M

M

L

R

R

E

V

Q

A

S

R

K

P

P

E

V

V

T

R

I

V

V

V

G

A

L

L

Q

E

P

P

E

S

E

N

G

A

S

A

-

M

-

L

-

A

-

R

-

G

-

E

-

W

S

S

I

P

P

H

G

Q

I

I

R

Q

R
x
G

W

L

P

A

T

P

E

N

Y

F

L

V

P

P

G

G

I

V

F

L

N

D

A

R

S

S

L

V

V

I

D

D

E

D

V

L

L

V

D

T

I

M

H

D

Q

E

R

V

D

T

A

A

E

R

N

D

T

T

M

S

R

R

E

R

L

L

A

A

V

A

R

E

E

E

G

G

I

I

F

F

C

A

G

G

V

I

S
|
S

S

A

G

G

G

A

A

T

V

V

A

A

G

T

A

A

L

L

R

S

V

I

A

A

K

E

A

H

N

A

P

P

D

E

-

G

A

T

V

V

V

L

A

A

I

M

I

L

C
x
P

D
|
D

R

T

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

F

V

L

F

F

active site: K42 (= K41), S264 (= S255)
binding pyridoxal-5'-phosphate: K42 (= K41), N72 (= N71), F175 (≠ M173), G176 (= G174), T177 (= T175), T178 (= T176), T180 (= T178), G220 (≠ R211), S264 (= S255), P290 (≠ C280), D291 (= D281)

D2Z027 O-ureido-L-serine synthase; Cysteine synthase homolog DscD; O-acetylserine sulfhydrylase; EC 2.6.99.3; EC 2.5.1.47 from Streptomyces lavendulae (see paper)
43% identity, 96% coverage: 1:292/303 of query aligns to 4:303/324 of D2Z027

query
sites
D2Z027

M

F

S

N

T

S

L

I

E

L

Q

D

T

T

I

I

G

G

N

R

T

T

P

P

L

I

V

V

K

R

L

L

Q

Q

R

R

M

M

G

A

P

P

D

E

N

H

G

T

S

S

E

-

V

V

W

Y

L

V

K

K

L

V

E

E

G

S

N

F

N

N

P

P

A

G

G

G

S

S

V

V

K
|
K

D

D

R

R

A

L

A

A

L

L

S

S

M

V

I

V

V

L

E

D

A

A

E

E

K

A

R

K

G

G

E

L

I

L

K

K

P

P

G

G

D

D

V

T

L

I

I

V

E

E

A

C

T

T

S

S

G

G

N

N

T
x
V

G

G

I

I

A

A

L

L

A

A

M

M

I

V

A

A

L

A

K

R

G

G

Y

Y

R

R

M

F

K

V

L

A

L

V

M

M

P

G

D

D

N

T

M
x
Y

S

S

Q

V

E

E

R

R

A

K

A

L

M

I

R

R

A

A

Y

Y

G

G

A

G

E

K

L

L

I

V

L

L

V

F

T

P

K

G

E

H

Q

L

G

G

M
x
S

E

K

G

G

A

G

R

N

D

L

L

I

A

A

L

D

E

E

M

L

A

A

N

E

R

K

G

Y

E

G

G

W

K

F

L

R

L

A

D

R

Q

Q

F

F

N

D

N

N

P

P

D

A

N

N

P

P

Y

S

A

Y

H

H

Y

R

T

E

T

T

G

A

P

S

E

E

I

I

W

L

Q

A

Q

D

T

F

G

A

G

G

-

K

R

R

I

L

T

D

H

H

F

F

V

V

S

T

S

G

M

F

G

G

T

T

G

G

T

T

I

L

T

T

G

G

V

V

S

G

R

Q

F

M

M

L

R

R

E

V

Q

A

S

R

K

P

P

E

V

V

T

R

I

V

V

V

G

A

L

L

Q

E

P

P

E

S

E

N

G

A

S

A

S

M

I

L

P

A

-

R

-

G

-

E

-

W

-

S

-

P

-

H

G

Q

I

I

R

Q

R

G

W

L

P

A

T

P

E

N

Y

F

L

V

P

P

G

G

I

V

F

L

N

D

A

R

S

S

L

V

V

I

D

D

E

D

V

L

L

V

D

T

I

M

H

D

Q

E

R

V

D

T

A

A

E

R

N

D

T

T

M

S

R

R

E

R

L

L

A

A

V

A

R

E

E

E

G

G

I

I

F

F

C

A

G

G

V

I

S

S

S

A

G

G

G

A

A

T

V

V

A

A

G

T

A

A

L

L

R

S

V

I

A

A

K

E

A

H

N

A

P

P

D

E

-

G

A

T

V

V

V

L

A

A

I

M

I

L

C

P

D

D

R

T

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

F

V

L

F

F

K43 (= K41) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Loss of catalytic activity, no longer binds N6-(pyridoxal phosphate)lysine.
V74 (≠ T72) mutation to T: KM for OAS is 61 mM, KM for H(2)S is unchanged.
Y97 (≠ M95) mutation to F: KM for OAS is unchanged, KM for H(2)S is 0.073 mM.; mutation to M: KM for OAS is 330 mM, KM for H(2)S is 0.084.
S121 (≠ M119) mutation to A: KM for OAS is 140 mM, KM for H(2)S is 0.095 mM.; mutation to M: KM for OAS is 44 mM, KM for H(2)S is 0.20 mM.

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
44% identity, 95% coverage: 6:292/303 of query aligns to 81:378/392 of P47999

query
sites
P47999

Q

Q

T

L

I

I

G

G

N

K

T

T

P

P

L

M

V

V

K

Y

L

L

Q

N

R

N

M

V

G

V

P

K

D

G

N

C

G

V

S

A

E

S

V

V

W

A

L

A

K

K

L

L

E

E

G

I

N

M

N

E

P

P

A

C

G

C

S

S

V

V

K

K

D

D

R

R

A

I

A

G

L

Y

S

S

M

M

I

I

V

T

E

D

A

A

E

E

K

E

R

K

G

G

E

L

I

I

K

T

P

P

G

G

-

K

D

S

V

V

L

L

I

V

E

E

A

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

I

I

A

A

L

S

K

K

G

G

Y

Y

R

K

M

L

K

I

L

L

L

T

M

M

P

P

D

A

N

S

M

M

S

S

Q

L

E

E

R

R

A

V

A

L

M

L

R

R

A

A

Y

F

G

G

A

A

E

E

L

L

I

V

L

L

V

T

T

E

K

P

E

A

Q

K

G

G

M

M

E

T

G

G

A

A

R

I

D

Q

L

K

A

A

L

E

E

E

M

I

A

L

N

K

R

K

G

T

E

P

G

N

K

S

-

Y

L

M

L

L

D

Q

Q

Q

F

F

N

D

N

N

P

P

D

A

N

N

P

P

Y

K

A

I

H

H

Y

Y

T

E

T

T

G

G

P

P

E

E

I

I

W

W

Q

E

Q

D

T

T

G

R

G

G

R

K

I

I

T

D

H

I

F

L

V

V

S

A

S

G

M

I

G

G

T

T

T

G

G

G

T

T

I

I

T

T

G

G

V

V

S

G

R

R

F

F

M

I

R

K

E

E

Q

R

S

K

K

P

P

E

V

L

T

K

I

V

V

I

G

G

L

V

Q

E

P

P

E

T

E

E

G

S

S

A

S

I

I

L

P

S

G

G

I

G

R

K

R

P

W

G

P

P

T

H

E

K

-

I

-

Q

-

G

-

I

-

G

-

A

-

G

Y

F

L

V

P

P

G

K

I

N

F

L

N

D

A

L

S

A

L

I

V

V

D

D

E

E

V

Y

L

I

D

A

I

I

H

S

Q

S

R

E

D

E

A

A

E

I

N

E

T

T

M

S

R

K

E

Q

L

L

A

A

V

L

R

Q

E

E

G

G

I

L

F

L

C

V

G

G

V

I

S

S

G

G

G

A

A

A

V

A

A

A

G

A

A

A

L

I

R

Q

V

V

A

A

K

K

-

R

-

P

A

E

N

N

P

A

D

G

A

K

V

L

V

I

V

A

A

V

I

V

I

F

C

P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

Q

V

L

F

F

Sites not aligning to the query:

61 modified: N-acetylalanine

8b9wA Cysteine synthase from trypanosoma theileri with plp bound (see paper)
42% identity, 96% coverage: 3:293/303 of query aligns to 12:312/329 of 8b9wA

query
sites
8b9wA

T

S

L

V

E

D

Q

A

T

L

I

I

G

G

N

N

T

T

P

P

L

A

V

V

K

Y

L

L

Q

R

R

N

M

M

G

N

P

-

D

K

N

T

G

A

S

A

E

T

V

I

W

V

L

L

K

K

L

L

E

E

G

S

N

E

N

N

P

P

A

M

G

A

S

S

V

V

K
|
K

D

D

R

R

A

L

A

A

L

Y

S

A

M

I

I

Y

V

D

E

K

A

A

E

E

K

K

R

E

G

G

E

K

I

I

K

K

P

R

G

G

-

K

D

S

V

V

L

I

I

V

E

E

A

A

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

L

I

L

A

G

A

A

L

V

K

R

G

G

Y

Y

R

K

M

V

K

I

L

I

L

T

M

M

P

P

D

D

N

S

M

M

S

S

Q

M

E

E

R

R

A

S

A

L

M

L

R

R

A

I

Y

F

G

G

A

A

E

E

L

L

I

I

L

L

V

T

T

P

K

A

E

S

Q

L

G

G

M

M

E

K

G

G

A

A

R

V

D

A

L

M

A

A

L

N

E

R

M

I

-

V

A

S

N

N

R

N

G

P

E

D

G

A

K

V

L

L

L

A

D

D

Q

Q

F

F

N

C

N

T

P

K

D

Y

N

N

P

A

Y

Q

A

I

H

H

Y

E

T

E

T

T

G

G

P

P

E

E

I

I

W

W

Q

R

Q

Q

T

T

G

K

G

G

R

H

I

V

T

D

H

C

F

F

V

V

S

A

S

G

M

V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

L

T

T

G

G

V

V

S

A

R

R

F

F

M

L

R

K

E

S

Q

V

S

G

K

C

P

N

V

A

T

N

I

I

V

V

G

A

L

V

Q

E

P

P

E

Q

E

E

G

S

S

P

S

V

I

L

P

S

G

G

I

G

R

R

R

P

W

G

P

P

T

H

E

R

-

I

-

Q

-
x
G

-

I

-

G

-

A

-

G

Y

F

L

V

P

P

G

E

I

V

F

L

N

D

A

R

S

E

L

L

V

V

D

D

E

E

V

I

L

I

D

Q

I

V

H

S

Q

S

R

D

D

K

A

A

E

I

N

E

T

T

M

A

R

Q

E

K

L

L

A

S

V

R

R

M

E

D

G

G

I

I

F

F

C

C

G

G

V

F

S
|
S

S

A

G

G

G

A

A

N

V

V

A

F

G

A

A

A

L

L

R

K

V

I

A

A

K

E

A

-

N

R

P

P

D

E

-

M

-

A

-

G

A

K

V

T

V

I

V

V

A

T

I

V

I

I

C
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

A

V

L

F

Y

G

A

binding pyridoxal-5'-phosphate: K49 (= K41), N80 (= N71), G184 (= G174), T185 (= T175), G186 (≠ T176), T188 (= T178), G228 (vs. gap), S272 (= S255), P299 (≠ C280)

3x44A Crystal structure of o-ureido-l-serine-bound k43a mutant of o-ureido- l-serine synthase (see paper)
43% identity, 96% coverage: 1:292/303 of query aligns to 3:302/321 of 3x44A

query
sites
3x44A

M

F

S

N

T

S

L

I

E

L

Q

D

T

T

I

I

G

G

N

R

T

T

P

P

L

I

V

V

K

R

L

L

Q

Q

R

R

M

M

G

A

P

P

D

E

N

H

G

T

S

S

E

-

V

V

W

Y

L

V

K

K

L

V

E

E

G

S

N

F

N

N

P

P

A

G

G

G

S

S

V

V

K
x
A

D

D

R

R

A

L

A

A

L

L

S

S

M

V

I

V

V

L

E

D

A

A

E

E

K

A

R

K

G

G

E

L

I

L

K

K

P

P

G

G

D

D

V

T

L

I

I

V

E

E

A

C

T
|
T

S
|
S

G

G

N
|
N

T
x
V

G

G

I

I

A

A

L

L

A

A

M

M

I

V

A

A

L

A

K

R

G

G

Y

Y

R

R

M

F

K

V

L

A

L

V

M

M

P

G

D

D

N

T

M

Y

S

S

Q

V

E

E

R

R

A

K

A

L

M

I

R

R

A

A

Y

Y

G

G

A

G

E

K

L

L

I

V

L

L

V

F

T

P

K

G

E

H

Q

L

G

G

M
x
S

E

K

G

G

A

G

R

N

D

L

L

I

A

A

L

D

E

E

M

L

A

A

N

E

R

K

G

Y

E

G

G

W

K

F

L

R

L

A

D

R

Q
|
Q

F

F

N

D

N

N

P

P

D

A

N

N

P

P

Y

S

A

Y

H

H

Y

R

T

E

T

T

G

A

P

S

E

E

I

I

W

L

Q

A

Q

D

T

F

G

A

G

G

-

K

R

R

I

L

T

D

H

H

F

F

V

V

S

T

S

G

M
x
F

G
|
G

T
|
T

G

G

T
|
T

I

L

T

T

G

G

V

V

S

G

R

Q

F

M

M

L

R

R

E

V

Q

A

S

R

K

P

P

E

V

V

T

R

I

V

V

V

G

A

L

L

Q

E

P

P

E

S

E

N

G

A

S

A

-

M

-

L

-

A

-

R

-

G

-

E

-

W

S

S

I

P

P

H

G

Q

I

I

R

Q

R
x
G

W
x
L

P

A

T
x
P

E

N

Y

F

L

V

P

P

G

G

I

V

F

L

N

D

A

R

S

S

L

V

V

I

D

D

E

D

V

L

L

V

D

T

I

M

H

D

Q

E

R

V

D

T

A

A

E

R

N

D

T

T

M

S

R

R

E

R

L

L

A

A

V

A

R

E

E

E

G

G

I

I

F

F

C

A

G

G

V

I

S
|
S

S

A

G

G

G

A

A

T

V

V

A

A

G

T

A

A

L

L

R

S

V

I

A

A

K

E

A

H

N

A

P

P

D

E

-

G

A

T

V

V

V

L

A

A

I

M

I

L

C
x
P

D
|
D

R

T

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

F

V

L

F

F

active site: A42 (≠ K41), S264 (= S255)
binding (E)-O-(carbamoylamino)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: T69 (= T68), S70 (= S69), N72 (= N71), V73 (≠ T72), S120 (≠ M119), Q141 (= Q140), F175 (≠ M173), G176 (= G174), T177 (= T175), T178 (= T176), T180 (= T178), G220 (≠ R211), L221 (≠ W212), P223 (≠ T214), S264 (= S255), P290 (≠ C280), D291 (= D281)

3vbeC Crystal structure of beta-cyanoalanine synthase in soybean (see paper)
40% identity, 96% coverage: 4:294/303 of query aligns to 15:316/329 of 3vbeC

query
sites
3vbeC

L

V

E

S

Q

Q

T

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

K

Y

L

L

Q

N

R

K

M

V

G

T

P

E

D

G

N

C

G

G

S

A

E

Y

V

V

W

A

L

V

K

K

L

Q

E

E

G

M

N

M

N

Q

P

P

A

T

G

A

S

S

V

I

K
|
K

D

D

R

R

A

P

A

A

L

Y

S

A

M

M

I

I

V

T

E

D

A

A

E

E

K

E

R

K

G

N

E

L

I

I

K

T

P

P

G

G

D

K

V

T

-

T

L

L

I

I

E

E

A

P

T

T

S
|
S

G

G

N
|
N

T

M

G

G

I

I

A

S

L

M

A

A

M

F

I

M

A

A

L

M

K

K

G

G

Y

Y

R

K

M

M

K

V

L

L

L

T

M

M

P

P

D

S

N

Y

M

T

S

S

Q

L

E

E

R

R

A

V

A

T

M

M

R

R

A

A

Y

F

G

G

A

A

E

E

L

L

I

I

L

L

V

T

T

D

K

P

E

A

Q

K

G

G

M

M

E

G

G

G

A

T

R

V

D

K

L

K

A

A

L

Y

E

E

M

L

-

L

A

E

N

N

R

T

G

P

E

N

G

A

K

H

L

M

L

L

D

Q

Q

Q

F

F

N

S

N

N

P

P

D

A

N

N

P

T

Y

Q

A

V

H

H

Y

F

T

E

T

T

G

G

P

P

E

E

I

I

W

W

Q

E

Q

D

T

T

G

N

G

G

R

Q

I

V

T

D

H

I

F

F

V

V

S
x
M

S

G

M

I

G
|
G

T
x
S

T
x
G

G

G

T
|
T

I

V

T

S

G

G

V

V

S

G

R

Q

F

Y

M

L

R

K

E

S

Q

K

S

N

K

P

P

N

V

V

T

K

I

I

V

Y

G

G

L

V

Q
x
E

P

P

E

S

E

E

G
x
S

S

N

S

V

I

L

P

N

G

G

I

G

R

K

R

P

W

G

P

P

T

H

E

H

-

I

-

T

-
x
G

-

N

-

G

-

V

-

G

Y

F

L

K

P

P

G

D

I

I

F

L

N

D

A

L

S

D

L

V

V

M

D

E

E

K

V

V

L

L

D

E

I

V

H

S

Q

S

R

E

D

D

A

A

E

V

N

N

T

M

M

A

R

R

E

V

L

L

A

A

V

L

R

K

E

E

G

G

I

L

F

M

C

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

N

V

T

A

V

G

A

A

A

L

L

R

R

V

L

A

A

K

Q

-

L

-

P

A

E

N

N

P

K

D

G

A

K

V

L

V

I

V

V

A

T

I

V

I

H

C
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

S

G

V

V

L

F

F

G

Q

E

E

active site: K52 (= K41), S81 (= S69), E212 (≠ Q199), S216 (≠ G203), S275 (= S255), P302 (≠ C280)
binding pyridoxal-5'-phosphate: K52 (= K41), N83 (= N71), M184 (≠ S171), G187 (= G174), S188 (≠ T175), G189 (≠ T176), T191 (= T178), G231 (vs. gap), S275 (= S255), P302 (≠ C280)

Q9FS29 Bifunctional L-3-cyanoalanine synthase/cysteine synthase 2, mitochondrial; EC 2.5.1.47; EC 4.4.1.9 from Solanum tuberosum (Potato) (see paper)
41% identity, 96% coverage: 2:292/303 of query aligns to 30:331/347 of Q9FS29

query
sites
Q9FS29

S

S

T

E

L

V

E

S

Q

Q

T

L

I

I

G

G

N

K

T

T

P

P

L

M

V

V

K

Y

L

L

Q

K

R

K

M

V

G

T

P

E

D

G

N

C

G

G

S

A

E

Y

V

I

W

A

L

V

K

K

L

Q

E

E

G

M

N

F

N

Q

P

P

A

T

G

S

S

S

V

I

K

K

D

D

R

R

A

P

A

A

L

L

S

A

M

M

I

I

V

N

E

D

A

A

E

E

K

K

R

K

G

G

E

L

I

I

K

S

P

P

-

E

G

K

D

T

V

T

L

L

I

I

E

E

A

P

T

T

S

S

G

G

N

N

T

M

G

G

I

I

A

S

L

M

A

A

M

F

I

M

A

A

L

M

K

K

G

G

Y

Y

R

K

M

M

K

V

L

L

L

T

M

M

P

P

D

S

N

Y

M

T

S

S

Q

M

E

E

R

R

A

V

A

T

M

M

R

R

A

A

Y

F

G

G

A

A

E

D

L

L

I

I

L

L

V

T

T

D

K

P

E

T

Q

K

G

G

M

M

E

G

G

G

A

T

R

V

D

K

L

K

A

A

L

Y

E
|
E

M

L

-

L

A

E

N

S

R

T

G

P

E

N

G

A

K

F

L

M

L

L

D

Q

Q

Q

F

F

N

S

N

N

P

P

D

A

N

N

P

T

Y

Q

A

V

H

H

Y

F

T

D

T

T

G

G

P

P

E

E

I

I

W

W

Q

E

T

T

G

L

G

G

R

N

I

V

T

D

H

I

F

F

V

V

S

M

S

G

M

I

G

G

T

S

T

G

G

G

T

T

I

V

T

T

G

G

V

V

S

G

R

L

F

Y

M

L

R

K

E

S

Q

K

S

N

K

P

P

N

V

V

T

K

I

I

V

Y

G

G

L

L

Q

E

P

P

E

T

E

E

G

S

S

N

S

I

I

L

P

N

G

G

I

G

R

K

R

P

W

G

P

P

T

H

E

H

Y

I

L

T

-

G

-

N

-

G

-

V

-

G

-

S

-

K

P

P

G

D

I

I

F

V

N

D

A

M

S

D

L

L

V

M

D

E

E

E

V

V

L

L

D

M

I

V

H

S

Q

S

R

E

D

D

A

A

E

V

N

N

T

M

M

A

R

R

E

E

L

L

A

A

V

V

R

K

E

E

G

G

I

L

F

M

C

V

G

G

V

I

S

S

G

G

G

A

A

N

V

T

A

V

G

A

A

A

L

L

R

R

V

L

A

A

K

Q

A

K

-

P

-

E

N

N

P

K

D

G

A

K

V

L

V

I

V

V

A

T

I

V

I

H

C

A

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

S

G

V

V

L

F

Y

E157 (= E128) mutation E->N,Q: No effect on catalytic activities.

Query Sequence

>16520 FitnessBrowser__Keio:16520
MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRAALSMIVEAEKRGEIKP
GDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAAMRAYGAELILVTKEQGME
GARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQQTGGRITHFVSSMGTTGTIT
GVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPTEYLPGIFNASLVDEVLDIHQRDAENT
MRELAVREGIFCGVSSGGAVAGALRVAKANPDAVVVAIICDRGDRYLSTGVFGEEHFSQG
AGI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory