SitesBLAST
Comparing 16525 FitnessBrowser__Keio:16525 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6qheA Alcohol dehydrogenase from arthrobacter sp. Ts-15 in complex with NAD+
43% identity, 99% coverage: 1:260/263 of query aligns to 2:261/261 of 6qheA
- binding nicotinamide-adenine-dinucleotide: G14 (= G13), M17 (≠ Q16), G18 (= G17), M19 (≠ I18), D38 (= D37), R39 (≠ I38), D63 (= D62), I64 (≠ V63), A90 (≠ N89), A91 (= A90), S142 (= S141), Y156 (= Y155), K160 (= K159), P186 (= P185), G187 (= G186), M189 (≠ V188), T191 (= T190), P192 (= P191), M193 (= M192)
Q8JZV9 Dehydrogenase/reductase SDR family member 6; (R)-beta-hydroxybutyrate dehydrogenase; 3-hydroxybutyrate dehydrogenase type 2; 4-oxo-L-proline reductase; Oxidoreductase UCPA; Short chain dehydrogenase/reductase family 15C member 1; EC 1.1.1.-; EC 1.1.1.30; EC 1.1.1.104 from Mus musculus (Mouse) (see paper)
39% identity, 97% coverage: 1:255/263 of query aligns to 1:245/245 of Q8JZV9
- Y147 (= Y155) active site, Proton acceptor; mutation to F: Loss of function.
D4A1J4 Dehydrogenase/reductase SDR family member 6; (R)-beta-hydroxybutyrate dehydrogenase; 3-hydroxybutyrate dehydrogenase type 2; 4-oxo-L-proline reductase; Oxidoreductase UCPA; Short chain dehydrogenase/reductase family 15C member 1; EC 1.1.1.-; EC 1.1.1.30; EC 1.1.1.104 from Rattus norvegicus (Rat) (see paper)
40% identity, 97% coverage: 1:255/263 of query aligns to 1:245/245 of D4A1J4
- Y147 (= Y155) mutation to F: Loss of function.
Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
37% identity, 96% coverage: 4:255/263 of query aligns to 3:245/248 of Q9KJF1
- S15 (≠ Q16) binding
- D36 (= D37) binding
- D62 (= D62) binding
- I63 (≠ V63) binding
- N89 (= N89) binding
- Y153 (= Y155) binding
- K157 (= K159) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
37% identity, 96% coverage: 4:255/263 of query aligns to 2:244/247 of 7pcsB
- binding nicotinamide-adenine-dinucleotide: G11 (= G13), M16 (≠ I18), D35 (= D37), I36 (= I38), I62 (≠ V63), N88 (= N89), G90 (= G91), I138 (≠ M139), S140 (= S141), Y152 (= Y155), K156 (= K159), I185 (≠ V188)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
38% identity, 96% coverage: 1:252/263 of query aligns to 2:241/244 of 4nbuB
- active site: G18 (= G17), N111 (= N113), S139 (= S141), Q149 (≠ E152), Y152 (= Y155), K156 (= K159)
- binding acetoacetyl-coenzyme a: D93 (≠ L95), K98 (≠ D100), S139 (= S141), N146 (≠ D149), V147 (≠ P150), Q149 (≠ E152), Y152 (= Y155), F184 (≠ Y187), M189 (= M192), K200 (≠ E211)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G13), N17 (≠ Q16), G18 (= G17), I19 (= I18), D38 (= D37), F39 (≠ I38), V59 (≠ A61), D60 (= D62), V61 (= V63), N87 (= N89), A88 (= A90), G89 (= G91), I90 (≠ V92), T137 (≠ M139), S139 (= S141), Y152 (= Y155), K156 (= K159), P182 (= P185), F184 (≠ Y187), T185 (≠ V188), T187 (= T190), M189 (= M192)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
37% identity, 95% coverage: 3:252/263 of query aligns to 2:243/246 of 3osuA
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
39% identity, 96% coverage: 2:254/263 of query aligns to 1:246/248 of 6ixmC
- active site: G16 (= G17), S142 (= S141), Y155 (= Y155), K159 (= K159)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), S15 (≠ Q16), G16 (= G17), I17 (= I18), D36 (= D37), I37 (= I38), A61 (= A61), D62 (= D62), T63 (≠ V63), N89 (= N89), A90 (= A90), M140 (= M139), S142 (= S141), Y155 (= Y155), K159 (= K159), P185 (= P185), A186 (≠ G186), Y187 (= Y187), I188 (≠ V188), L192 (≠ M192)
6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
38% identity, 95% coverage: 4:252/263 of query aligns to 3:240/244 of 6t77A
- active site: G16 (= G17), S138 (= S141), Y151 (= Y155)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G13), S14 (≠ L15), R15 (≠ Q16), T37 (≠ I38), L58 (≠ A61), N59 (≠ D62), V60 (= V63), A87 (= A90), G88 (= G91), I89 (≠ V92)
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
37% identity, 94% coverage: 7:252/263 of query aligns to 2:236/239 of 3sj7A
- active site: G12 (= G17), S138 (= S141), Q148 (≠ E152), Y151 (= Y155), K155 (= K159)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G13), S10 (≠ L15), R11 (≠ Q16), I13 (= I18), N31 (vs. gap), Y32 (vs. gap), A33 (≠ L36), G34 (≠ D37), S35 (≠ I38), A58 (= A61), N59 (≠ D62), V60 (= V63), N86 (= N89), A87 (= A90), T109 (≠ I112), S138 (= S141), Y151 (= Y155), K155 (= K159), P181 (= P185), G182 (= G186)
6ci9D Rmm microcompartment-associated aminopropanol dehydrogenase NADP + aminoacetone holo-structure (see paper)
34% identity, 97% coverage: 3:258/263 of query aligns to 6:254/259 of 6ci9D
- active site: G20 (= G17), S145 (= S141), Y159 (= Y155)
- binding 1-aminopropan-2-one: F97 (≠ C93), S145 (= S141), T147 (= T143), W156 (≠ E152), Y159 (= Y155), G190 (= G186)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G16 (= G13), S18 (≠ L15), G20 (= G17), I21 (= I18), G40 (≠ D37), R41 (≠ I38), N42 (≠ S39), D66 (= D62), V67 (= V63), N93 (= N89), G95 (= G91), T143 (≠ M139), S145 (= S141), Y159 (= Y155), K163 (= K159), P189 (= P185), N191 (≠ Y187), I192 (≠ V188), T194 (= T190), G196 (≠ M192), L197 (≠ A193)
5yssB Crystal structure of aminocaproic acid cyclase in complex with NAD (+) (see paper)
38% identity, 95% coverage: 3:252/263 of query aligns to 1:251/255 of 5yssB
- binding nicotinamide-adenine-dinucleotide: G11 (= G13), T13 (≠ L15), S14 (≠ Q16), G15 (= G17), I16 (= I18), G35 (≠ D37), F36 (≠ I38), L60 (≠ V63), N86 (= N89), G88 (= G91), I89 (≠ V92), A137 (≠ S140), Y151 (= Y155), K155 (= K159), P181 (= P185), G182 (= G186), V184 (= V188), T186 (= T190)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
37% identity, 96% coverage: 4:255/263 of query aligns to 3:247/247 of 4jroC
- active site: G16 (= G17), S142 (= S141), Q152 (≠ E152), Y155 (= Y155), K159 (= K159)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G13), S14 (≠ L15), R15 (≠ Q16), G16 (= G17), I17 (= I18), N35 (vs. gap), Y36 (≠ L36), N37 (≠ D37), G38 (≠ I38), S39 (= S39), N63 (≠ D62), V64 (= V63), N90 (= N89), A91 (= A90), I93 (≠ V92), I113 (= I112), S142 (= S141), Y155 (= Y155), K159 (= K159), P185 (= P185), I188 (≠ V188), T190 (= T190)
4nbtA Crystal structure of fabg from acholeplasma laidlawii (see paper)
36% identity, 96% coverage: 3:255/263 of query aligns to 2:239/239 of 4nbtA
- active site: G16 (= G17), S132 (= S141), Y145 (= Y155), K149 (= K159)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), K15 (≠ Q16), G16 (= G17), L17 (≠ I18), D36 (= D37), L37 (≠ I38), L52 (≠ A61), N53 (≠ D62), V54 (= V63), N80 (= N89), A81 (= A90), G82 (= G91), I130 (≠ M139), S132 (= S141), Y145 (= Y155), K149 (= K159), P177 (= P185), G178 (= G186), I180 (≠ V188), T182 (= T190)
2ag5A Crystal structure of human dhrs6 (see paper)
39% identity, 97% coverage: 1:255/263 of query aligns to 1:245/246 of 2ag5A
- active site: S133 (= S141), Y147 (= Y155), K151 (= K159), R192 (≠ Q199)
- binding nicotinamide-adenine-dinucleotide: Q16 (= Q16), G17 (= G17), I18 (= I18), D37 (= D37), I38 (= I38), D58 (= D62), V59 (= V63), V81 (≠ N89), G83 (= G91), L104 (≠ I112), Y147 (= Y155), K151 (= K159), P177 (= P185), V180 (= V188), T182 (= T190), S184 (vs. gap)
- binding sulfate ion: R144 (≠ E152), R188 (≠ S195), F202 (≠ M212), R205 (≠ A215)
Q9BUT1 Dehydrogenase/reductase SDR family member 6; (R)-beta-hydroxybutyrate dehydrogenase; 3-hydroxybutyrate dehydrogenase type 2; 4-oxo-L-proline reductase; Oxidoreductase UCPA; Short chain dehydrogenase/reductase family 15C member 1; EC 1.1.1.-; EC 1.1.1.30; EC 1.1.1.104 from Homo sapiens (Human) (see 4 papers)
39% identity, 97% coverage: 1:255/263 of query aligns to 1:245/245 of Q9BUT1
7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
36% identity, 96% coverage: 3:255/263 of query aligns to 5:247/247 of 7tzpG