SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 16639 FitnessBrowser__Keio:16639 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 12 hits to proteins with known functional sites (download)

2e4pA Crystal structure of bpha3 (oxidized form) (see paper)
46% identity, 93% coverage: 6:104/106 of query aligns to 5:104/108 of 2e4pA

query
sites
2e4pA

A
|
A

C

C

P

S

V

V

A

D

D
x
E

V

V

P

P

E

P

G

G

E

E

A

A

L

L

R

Q

I

V

D

S

-

H

T

D

S

A

P

Q

V

K

I

V

A

A

L

I

F

F

N

N

V

V

G

D

G

G

E

E

F

F

Y

F

A

A

I

T

N

Q

D

D

R

Q

C
|
C

S

T

H
|
H

G
|
G

N

E

A

W

S

S

M

L

S

S

E

E

G

G

Y

G

L

Y

E

L

D

D

D

G

A

D

T

V

V

V

E

E

C
|
C

P

S

L

L

H
|
H

A

M

A
x
G

S

K

F

F

C

C

L

V

K

R

T

T

G

G

K

K

A

V

L

K

C

S

L

P

P

P

A

P

T

C

D

E

P

P

L

L

T

K

T

V

Y

Y

P

P

V

I

H

R

V

I

E

E

G

G

G

R

D

D

I

V

F

L

I

V

D

D

L

F

P

S

E

R

A

A

binding fe2/s2 (inorganic) cluster: C42 (= C42), H44 (= H44), G45 (= G45), C62 (= C62), H65 (= H65), G67 (≠ A67)
binding alpha-D-glucopyranose: A5 (= A6), E10 (≠ D11)

1fqtA Crystal structure of the rieske-type ferredoxin associated with biphenyl dioxygenase (see paper)
44% identity, 95% coverage: 1:101/106 of query aligns to 1:102/109 of 1fqtA

query
sites
1fqtA

M

M

N

K

R

F

I

T

Y

R

A

V

C

C

P

D

V

R

A

R

D

D

V

V

P

P

E

E

G

G

E

E

A

A

L

L

R

K

I

V

D

E

T

S

S

G

P

G

V

T

-

S

I

V

A

A

L

I

F

F

N

N

V

V

G

D

G

G

E

E

F

L

Y

F

A

A

I

T

N

Q

D

D

R

R

C
|
C

S

T

H
|
H

G
|
G

N

D

A

W

S

S

M

L

S

S

E

D

-

G

G

G

Y

Y

L

L

E

E

D

G

D

D

A

-

T

V

V

V

E

E

C
|
C

P

S

L

L

H
|
H

A

M

A
x
G

S

K

F

F

C

C

L

V

K

R

T

T

G

G

K

K

A

V

L

K

C

S

L

P

P

P

A
x
P

T

C

D

E

P

A

L

L

T

K

T

I

Y

F

P

P

V

I

H

R

V

I

E

E

G

D

G

N

D

D

I

V

F

L

I

V

D

D

L

F

binding fe2/s2 (inorganic) cluster: C43 (= C42), H45 (= H44), G46 (= G45), C63 (= C62), H66 (= H65), G68 (≠ A67), P82 (≠ A81)

3dqyA Crystal structure of toluene 2,3-dioxygenase ferredoxin (see paper)
41% identity, 95% coverage: 5:105/106 of query aligns to 4:104/106 of 3dqyA

query
sites
3dqyA

Y

Y

A

I

C

L

P

R

V

Q

A

G

D

D

V

L

P

P

E

P

G

G

E

E

A

M

L

Q

R

R

I

Y

D

E

T

G

S

G

P

P

V

E

-

P

I

V

A

M

L

V

F

C

N

N

V

V

G

D

G

G

E

E

F

F

Y

F

A

A

I

V

N

Q

D

D

R

T

C
|
C

S

T

H
|
H

G
|
G

N

D

A

W

S

A

M

L

S

S

E

D

G

G

Y

Y

L

L

E

D

D

G

D

D

A

-

T

I

V

V

E

E

C
|
C

P

T

L

L

H
|
H

A

F

A
x
G

S

K

F

F

C

C

L

V

K

R

T

T

G

G

K

K

A

V

L

K

C

A

L

L

P

P

A

A

T

C

D

K

P

P

L

I

T

K

T

V

Y

F

P

P

V

I

H

K

V

V

E

E

G

G

G

D

D

E

I

V

F

H

I

V

D

D

L

L

P

D

E

N

A

G

Q

E

binding fe2/s2 (inorganic) cluster: C42 (= C42), H44 (= H44), G45 (= G45), C61 (= C62), H64 (= H65), G66 (≠ A67)

P0A185 Naphthalene 1,2-dioxygenase system, ferredoxin component from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
44% identity, 95% coverage: 1:101/106 of query aligns to 3:103/104 of P0A185

query
sites
P0A185

M

V

N

K

R

W

I

I

Y

E

A

A

C

V

P

A

V

L

A

S

D

D

V

I

P

L

E

E

G

G

E

D

A

V

L

L

R

G

I

V

D

T

T

V

S

E

-

G

P

K

V

E

I

L

A

A

L

L

F

Y

N

E

V

V

G

E

G

G

E

E

F

I

Y

Y

A

A

I

T

N

D

D

N

R

L

C
|
C

S

T

H
|
H

G

G

N

S

A

A

S

R

M

M

S

S

E

D

G

G

Y

Y

L

L

E

E

D

G

D

-

A

R

T

E

V

I

E

E

C
|
C

P

P

L

L

H
|
H

A

Q

A

G

S

R

F

F

C

D

L

V

K

C

T

T

G

G

K

K

A

A

L

L

C

C

L

A

P

P

A

V

T

T

D

Q

P

N

L

I

T

K

T

T

Y

Y

P

P

V

V

H

K

V

I

E

E

G

N

G

L

D

R

I

V

F

M

I

I

D

D

L

L

C45 (= C42) binding
H47 (= H44) binding
C64 (= C62) binding
H67 (= H65) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2qpzA Naphthalene 1,2-dioxygenase rieske ferredoxin (see paper)
44% identity, 95% coverage: 1:101/106 of query aligns to 2:102/103 of 2qpzA

query
sites
2qpzA

M

V

N

K

R

W

I

I

Y

E

A

A

C

V

P

A

V

L

A

S

D

D

V

I

P

L

E

E

G

G

E

D

A

V

L

L

R

G

I

V

D

T

T

V

S

E

-

G

P

K

V

E

I

L

A

A

L

L

F

Y

N

E

V

V

G

E

G

G

E

E

F

I

Y

Y

A

A

I

T

N

D

D

N

R

L

C
|
C

S

T

H
|
H

G
|
G

N

S

A

A

S

R

M

M

S

S

E

D

G

G

Y

Y

L

L

E

E

D

G

D

-

A

R

T

E

V

I

E

E

C
|
C

P

P

L
|
L

H
|
H

A

Q

A
x
G

S

R

F

F

C

D

L

V

K

C

T

T

G

G

K

K

A

A

L

L

C

C

L

A

P
|
P

A

V

T

T

D

Q

P

N

L

I

T

K

T

T

Y

Y

P

P

V

V

H

K

V

I

E

E

G

N

G

L

D

R

I

V

F

M

I

I

D

D

L

L

binding fe2/s2 (inorganic) cluster: C44 (= C42), H46 (= H44), G47 (= G45), C63 (= C62), L65 (= L64), H66 (= H65), G68 (≠ A67), P81 (= P80)

2i7fA Sphingomonas yanoikuyae b1 ferredoxin (see paper)
41% identity, 95% coverage: 2:102/106 of query aligns to 1:102/102 of 2i7fA

query
sites
2i7fA

N

N

R

K

I

L

Y

R

A

L

C

C

P

Q

V

V

A

A

D

S

V

V

P

K

E

D

G

G

E

E

-

P

-

V

A

A

L

V

R

Y

I

Q

D

E

T

K

S

M

P

P

V

A

I

L

A

A

L

V

F

Y

N

N

V

V

G

D

G

G

E

E

F

V

Y

F

A

V

I

T

N

D

D

N

R

L

C
|
C

S

T

H
|
H

G
|
G

N

N

A

A

S

M

M

L

S

T

E

D

G

G

Y

Y

L

-

E

Q

D

D

D

G

A

T

T

I

V

I

E

E

C
|
C

P

P

L
x
F

H
|
H

A

G

A

G

S

S

F

F

C

D

L

I

K

A

T

T

G

G

K

A

A

A

L

K

C

A

L

F

P

P

A

C

T

Q

D

I

P

P

L

I

T

K

T

T

Y

Y

P

P

V

V

H

T

V

I

E

E

G

D

G

G

D

W

I

V

F

C

I

I

D

D

L

Q

P

P

binding fe2/s2 (inorganic) cluster: C43 (= C42), H45 (= H44), G46 (= G45), C62 (= C62), F64 (≠ L64), H65 (= H65)

5bokA Ferredoxin component of 3-nitrotoluene dioxygenase from diaphorobacter sp. Strain ds2
45% identity, 94% coverage: 2:101/106 of query aligns to 2:101/102 of 5bokA

query
sites
5bokA

N

N

R

W

I

I

Y

D

A

A

C

A

P

A

V

R

A

D

D

E

V

V

P

P

E

E

G

G

E

D

A

V

L

I

R

G

I

I

D

N

-

I

T

V

S

G

P

K

V

E

I

I

A

A

L

L

F

Y

N

E

V

V

G

A

G

G

E

E

F

I

Y

Y

A

A

I

T

N

D

D

N

R

T

C
|
C

S

T

H
|
H

G
|
G

N

A

A
|
A

S

R

M

M

S

S

E

D

G

G

Y

F

L

L

E

E

D

G

D

-

A

R

T

E

V

I

E

E

C
|
C

P

P

L
|
L

H
|
H

A

Q

A

G

S

R

F

F

C

D

L

V

K

C

T

T

G

G

K

K

A

A

L

L

C

C

L

T

P
|
P

A

L

T

T

D

Q

P

D

L

I

T

K

T

T

Y

Y

P

P

V

V

H

K

V

I

E

E

G

N

G

M

D

R

I

V

F

M

I

L

D

K

L

L

binding fe2/s2 (inorganic) cluster: C43 (= C42), H45 (= H44), G46 (= G45), A48 (= A47), C62 (= C62), L64 (= L64), H65 (= H65), P80 (= P80)

Q8GI16 Ferredoxin CarAc; Carbazole 1,9a-dioxygenase, ferredoxin component; CARDO from Pseudomonas resinovorans (see 2 papers)
37% identity, 93% coverage: 1:99/106 of query aligns to 1:102/107 of Q8GI16

query
sites
Q8GI16

M

M

N

N

R

Q

I

I

Y

W

-

L

-

K

A

V

C

C

P

A

V

A

A

S

D

D

V

M

P

Q

E

P

G

G

E

T

A

I

L

R

R

R

I

V

D

N

T

R

-

V

-

G

S

A

P

A

V

P

I

L

A

A

L

V

F

Y

N

R

V

V

G

G

G

D

E

Q

F

F

Y

Y

A

A

I

T

N

E

D

D

R

T

C
|
C

S

T

H
|
H

G

G

N

I

A

A

S

S

M

L

S

S

E

E

G

G

Y

T

L

L

E

D

D

G

D

D

A

-

T

V

V

I

E

E

C
|
C

P

P

L

F

H
|
H

A

G

A

G

S

A

F

F

C

N

L

V

K

C

T

T

G

G

K

M

A

P

L

A

C

S

L

S

P

P

A

C

T

T

D

V

P

P

L

L

T

G

T

V

Y

F

P

E

V

V

H

E

V

V

E

K

G

E

G

G

D

E

I

V

F

Y

I

V

C46 (= C42) binding
H48 (= H44) binding
C65 (= C62) binding
H68 (= H65) binding

4nbbE Carbazole- and oxygen-bound oxygenase with ile262 replaced by val and ferredoxin complex of carbazole 1,9a-dioxygenase (see paper)
36% identity, 92% coverage: 2:99/106 of query aligns to 1:101/114 of 4nbbE

query
sites
4nbbE

N

N

R

Q

I

I

Y

W

-

L

-

K

A

V

C

C

P

A

V

A

A

S

D

D

V

M

P

Q

E

P

G

G

E

T

A

I

L

R

R

R

I

V

D

N

T

R

-

V

-

G

S

A

P

A

V

P

I

L

A

A

L

V

F

Y

N

R

V

V

G

G

G

D

E

Q

F

F

Y

Y

A

A

I

T

N

E

D

D

R

T

C
|
C

S

T

H
|
H

G
|
G

N

I

A

A

S

S

M

L

S

S

E

E

G

G

Y

T

L

L

E

D

D

G

D

D

A

-

T

V

V

I

E

E

C
|
C

P

P

L
x
F

H
|
H

A

G

A
x
G

S

A

F

F

C

N

L

V

K

C

T

T

G

G

K

M

A

P

L

A

C

S

L

S

P

P

A

C

T

T

D

V

P

P

L

L

T

G

T

V

Y

F

P

E

V

V

H

E

V

V

E

K

G

E

G

G

D

E

I

V

F

Y

I

V

binding fe2/s2 (inorganic) cluster: C45 (= C42), H47 (= H44), G48 (= G45), C64 (= C62), F66 (≠ L64), H67 (= H65), G69 (≠ A67)

3gceA Ferredoxin of carbazole 1,9a-dioxygenase from nocardioides aromaticivorans ic177 (see paper)
33% identity, 78% coverage: 17:99/106 of query aligns to 19:101/104 of 3gceA

query
sites
3gceA

A

A

L

F

R

D

I

V

D

D

T

G

S

D

P

E

V

V

I

M

A

V

L

V

F

R

N

D

V

-

G

G

G

D

E

S

F

V

Y

Y

A

A

I

I

N

S

D

N

R

L

C
|
C

S

S

H
|
H

G
x
A

N

E

A

A

S

Y

M

L

S

D

E

M

G

G

Y

V

L

F

E

H

D

A

D

E

A

S

T

L

-

E

V

I

E

E

C
|
C

P

P

L

L

H
|
H

A

V

A
x
G

S

R

F

F

C

D

L

V

K

R

T

T

G

G

K

A

A

P

L

T

C

A

L

L

P
|
P

A

C

T

V

D

L

P

P

L

V

T

R

T

A

Y

Y

P

D

V

V

H

V

V

V

E

D

G

G

G

T

D

E

I

I

F

L

I

V

binding fe2/s2 (inorganic) cluster: C43 (= C42), H45 (= H44), A46 (≠ G45), C64 (= C62), H67 (= H65), G69 (≠ A67), P82 (= P80)

1z03A 2-oxoquinoline 8-monooxygenase component: active site modulation by rieske-[2fe-2s] center oxidation/reduction (see paper)
34% identity, 74% coverage: 26:103/106 of query aligns to 53:144/427 of 1z03A

query
sites
1z03A

I

I

A

V

L

L

F

R

N

R

V

V

G

N

G

G

E

K

F

V

Y

F

A

A

I

L

N

K

D

D

R

Q

C
|
C

S

L

H
|
H

G
x
R

N

G

A
x
V

S

R

M

L

S

S

E

E

G

K

-

P

Y

T

L

C

E

F

D

T

D

K

A

S

T

T

V

I

E

S

C
|
C

P

W

L

Y

H
|
H

A

G

A
x
F

S

T

F

F

C

D

L

L

K

E

T

T

G

G

K

K

A

L

L

V

C

T

L

I

P

V

A

A

-

N

-

P

-

E

-

D

-

K

-

L

-

I

-

G

T

T

D

T

P

G

L

V

T

T

T

T

Y

Y

P

P

V

V

H

H

V

E

E

V

G

N

G

G

D

M

I

I

F

F

I

V

-

F

-

V

-

R

-

E

-

D

D

D

L

F

P

P

E

D

binding fe2/s2 (inorganic) cluster: C69 (= C42), H71 (= H44), R72 (≠ G45), V74 (≠ A47), C90 (= C62), H93 (= H65), F95 (≠ A67)

Sites not aligning to the query:

binding fe (iii) ion: 206, 210, 350
binding quinolin-2(1h)-one: 201, 277, 289, 292, 299

1z01A 2-oxoquinoline 8-monooxygenase component: active site modulation by rieske-[2fe-2s] center oxidation/reduction (see paper)
34% identity, 74% coverage: 26:103/106 of query aligns to 53:144/427 of 1z01A

query
sites
1z01A

I

I

A

V

L

L

F

R

N

R

V

V

G

N

G

G

E

K

F

V

Y

F

A

A

I

L

N

K

D

D

R

Q

C
|
C

S

L

H
|
H

G
x
R

N

G

A
x
V

S

R

M

L

S

S

E

E

G

K

-

P

Y

T

L

C

E

F

D

T

D

K

A

S

T

T

V

I

E

S

C
|
C

P

W

L

Y

H
|
H

A

G

A
x
F

S

T

F

F

C

D

L

L

K

E

T

T

G

G

K

K

A

L

L

V

C

T

L

I

P

V

A

A

-

N

-

P

-

E

-

D

-

K

-

L

-

I

-

G

T

T

D

T

P

G

L

V

T

T

T

T

Y

Y

P

P

V

V

H

H

V

E

E

V

G

N

G

G

D

M

I

I

F

F

I

V

-

F

-

V

-

R

-

E

-

D

D

D

L

F

P

P

E

D

binding fe2/s2 (inorganic) cluster: C69 (= C42), H71 (= H44), R72 (≠ G45), V74 (≠ A47), C90 (= C62), H93 (= H65), F95 (≠ A67)

Sites not aligning to the query:

binding fe (iii) ion: 206, 210, 350

Query Sequence

>16639 FitnessBrowser__Keio:16639
MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSHGNASMSEGYLEDDATV
ECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLPEAQP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory