SitesBLAST

R102 (= R131) mutation to A: Loss of transport activity.
I105 (≠ Q134) mutation to S: Affects symport activity. May function as an uniporter.
E122 (= E151) mutation to A: Loss of transport activity.
Q137 (≠ Y166) mutation to A: Loss of transport activity.
Q250 (vs. gap) mutation to A: Loss of transport activity.
Q251 (vs. gap) mutation to A: Loss of transport activity.
N256 (≠ G255) mutation to A: Loss of transport activity.
W357 (vs. gap) mutation to A: Loss of transport activity.

Sites not aligning to the query:

22 D→N: Affects symport activity. May function as an uniporter.

Q9Y7Q9 Probable metabolite transporter C2H8.02 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
24% identity, 54% coverage: 3:234/432 of query aligns to 21:262/583 of Q9Y7Q9

query
sites
Q9Y7Q9

E

E

S

Q

T

Y

V

E

T

A

A

S

D

E

S

Q

K

K

L

I

T

L

S

G

S

L

D

V

T

T

R

K

R

E

I

A

W

K

A

L

I

L

V

A

G

I

A

A

S

G

S

T

G

G

N

F

L

L

V

L

E

D

W

S

F

Y

D

D

F

L

Y

F

V

I

Y

I

S

N

F

L

C

V

S

S

L

P

Y

I

F

L

A

A

H

Y

I

L

F

Y

F

W

P

-

S

-

G

G

N

G

T

L

T

T

T

G

Q

H

L

Q

L

D

Q

Y

T

P

A

S

G

G

-

I

-

R

-

G

-

V

V

V

F

N

A

A

G

T

F

N

L

I

M

G

R

N

P

I

I

M

G

G

G

Q

W

L

L

L

F

F

G

G

R

F

I

L

A

G

D

D

K

F

H

F

G

G

R

R

K

K

K

F

S

V

M

Y

L

G

L

K

S

E

V

M

C

M

M

V

C

I

F

I

G

A

S

T

L

I

V

L

I

I

A

I

C

C

L

L

P

P

G

D

Y

R

E

I

T

P

I

T

G

P

T

T

W

A

A

K

P

M

A

M

L

W

L

L

L

F

L

A

A

F

R

R

L

V

F

M

Q

L

G

G

L

I

S

G

V

I

G

G

E

D

Y

Y

G

P

T

M

S

S

A

A

T

S

Y

I

M

T

S

S

E

E

V

Q

A

S

V

L

E

I

G

N

R

R

K

R

G

G

-

A

-

L

-

A

F

W

Y

I

A

F

S

S

F

N

Q

Q

Y

G

V

W

T

G

L

T

I

L

G

A

G

G

Q

C

L

V

L

A

A

T

L

L

L

I

V

I

V

L

V

A

V

C

L

F

Q

E

H

K

T

P

M

L

E

N

D

D

A

R

A

G

L

-

R

-

E

E

W

Y

G

T

-

K

-

L

-

N

-

G

-

V

W

W

R

R

I

I

P

Q

F

F

A

G

L

I

G

A

A

L

V

F

L

P

A

A

V

V

V

I

A

V

L

L

W

I

L

P

R

R

R

L

Q

R

L

M

D

Q

E

E

T

S

S

E

Q

Q

-

F

Q

K

E

N

T

S

R

K

A

N

L

M

K

K

E

S

A

P

G

G

S

E

Sites not aligning to the query:

267 modified: Phosphoserine
269 modified: Phosphoserine
289 modified: Phosphoserine
290 modified: Phosphoserine
292 modified: Phosphoserine
330 modified: Phosphoserine

O23492 Inositol transporter 4; Myo-inositol-proton symporter INT4; Protein INOSITOL TRANSPORTER 4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
28% identity, 34% coverage: 86:231/432 of query aligns to 90:228/582 of O23492

query
sites
O23492

I

I

A

N

D

D

K

K

H

F

G

G

R

R

K

R

K

M

S

S

M

I

L

L

L

I

S

A

V

D

C

V

M

L

M

F

C

L

F

I

G

G

S

A

L

I

V

V

I

M

A

A

C

F

L

A

P

P

G

-

Y

-

E

-

T

-

I

-

G

-

T

-

W

-

A

A

P

P

A

W

L

V

L

I

L

V

A

G

R

R

L

I

F

F

Q

V

G

G

L

F

S

G

V

V

G

G

G

M

E

A

Y

S

G

M

T

T

S

S

A

P

T

L

Y

Y

M

I

S

S

E

E

V

A

A

S

V

P

E

A

G

R

R

I

K

R

G

G

F

A

Y

L

A

V

S

S

F

T

Q

N

Y

G

V

L

T

L

L

I

I

T

G

G

Q

Q

L

F

A

S

L

Y

L

L

V

I

V

N

V

L

V

A

L

F

Q

V

H

H

T

T

M

P

E

G

D

T

A

-

L

-

R

-

E

-

W

-

G

-

W

W

R

R

I

W

P

M

F

L

A

G

L

V

G

A

A

G

V

V

L

P

A

A

V

I

V

V

A

Q

L

F

W

V

L

L

R

M

R

L

Q

S

L

L

D

P

E

E

T

S

S

P

Q

R

-

W

-

L

-

Y

-

R

-

K

-

D

-

R

-

I

Q

A

E

E

T

S

R

R

A

A

L

I

K

L

E

E

Sites not aligning to the query:

559:561 LLE→AAA: No effect on targeting.
559:582 mutation Missing: No effect on targeting.
564:565 FK→AA: No effect on targeting.
570:575 RRREKK→AAAAAA: No effect on targeting.

Q9C757 Probable inositol transporter 2 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
24% identity, 58% coverage: 88:336/432 of query aligns to 93:386/580 of Q9C757

query
sites
Q9C757

D

D

K

K

H

L

G

G

R

R

K

R

K

S

S

A

M

I

L

L

L

M

S

A

V

D

C

F

M

L

M

F

C

L

F

L

G

G

S

A

L

I

V

I

I

M

A

A

C

A

L

A

P

P

G

N

Y

-

E

-

T

-

I

-

G

-

T

-

W

-

A

-

P

P

A

S

L

L

L

V

A

G

R

R

L

V

F

F

Q

V

G

G

L

L

S

G

V

V

G

G

G

M

E

A

Y

S

G

M

T

T

S

A

A

P

T

L

Y

Y

M

I

S

S

E

E

V

A

A

S

V

P

E

A

G

K

R

I

K

R

G

G

F

A

Y

L

A

V

S

S

F

T

Q

N

Y

G

V

F

T

L

L

I

I

T

G

G

Q

Q

L

F

L

L

A

S

L

Y

L

L

V

I

V

N

V

L

V

A

L

F

Q

T

H

D

T

V

M

T

E

G

D

T

A

-

L

-

R

-

E

-

W

W

G

R

W

W

R

M

-

L

-

G

-

I

-

A

-

G

I

I

P

P

-

A

-

L

-

Q

F

F

A

V

L

L

G

M

A

F

V

T

L

L

A

P

V

E

V

S

A

P

L

R

W

W

L

L

R

Y

R

R

Q

K

L

G

D

R

E

E

T

E

S

E

-

A

-

K

-

A

-

I

-

L

-

R

-

I

-

Y

-

S

-

A

-

E

-

D

-

V

Q

E

Q

Q

E

E

T

I

R

R

A

A

L

L

K

K

E

D

A

S

G

V

S

E

L

T

K

E

G

I

L

L

W

E

R

E

N

G

-

S

-

E

-

K

-

I

-

N

-

M

-

I

-

K

-

L

-

C

-

K

-

A

-

K

-

T

-

V

R

R

A

G

F

L

I

I

M

A

V

G

L

V

G

G

F

L

T

Q

A

V

A

F

G

Q

S

Q

L

F

C

V

F

G

Y

I

T

N

F

T

T

V

T

M

Y

Y

M

Y

Q

S

K

P

Y

T

L

I

V

V

N

Q

T

L

A

A

G

G

M

F

H

A

A

S

N

N

V

R

A

T

S

A

-

L

G

S

I

L

M

V

T

T

A

A

A

G

L

L

F

N

V

A

F

F

M

G

L

S

I

I

Q

I

P

S

L

I

I

Y

G

-

A

-

L

F

S

I

D

D

K

R

I

I

G

G

R

R

R

K

T

K

-

L

-

I

-

S

-

L

-

F

-

G

S

V

M

I

L

I

C

S

F

L

G

G

S

I

L

L

A

T

A

G

I

V

F

F

-

Y

-

E

-

A

-

T

T

H

V

A

P

P

I

A

L

I

S

S

A

S

L

L

Q

E

-

T

-

Q

-

R

-

F

-

N

N

N

V

I

S

S

S

C

P

P

Sites not aligning to the query:

399 C→A: Strongly decreased nickel inhibition; when associated with A-402, A-410 and A-413.; C→S: No effect on inostol transport or nickel inhibition. No effect on inostol transport or nickel inhibition; when associated with S-410.
402 C→A: Strongly decreased nickel inhibition; when associated with A-399, A-410 and A-413.
410 C→A: Strongly decreased nickel inhibition; when associated with A-399, A-402 and A-413.; C→S: No effect on inostol transport or nickel inhibition; when associated with S-399.
413 C→A: Strongly decreased nickel inhibition; when associated with A-399, A-402 and A-410.

8fvzA Pipt y150a
24% identity, 44% coverage: 27:215/432 of query aligns to 9:200/433 of 8fvzA

query
sites
8fvzA

A

A

S

G

S

V

G

G

N

F

L

F

V

L

E

D

W

A

F

Y

D

D

F

L

Y

F

V

I

Y

I

S

N

F

Q

C

V

S

A

L

P

Y

M

F

L

A

A

H

Q

I

V

F

Y

F

F

P

P

S

K

G

T

N

G

T

L

T

P

T

A

Q

Q

L

-

Q

R

T

Q

A

D

G

L

V

M

F

K

A

A

G

A

F

N

L

I

M

G

R

C

P

V

I

V

G

G

G

Q

W

V

L

M

F

F

G

G

R

V

I

L

A

G

D

D

K

S

H

F

G

G

R

R

K

K

-

F

-

V

-

Y

-

G

K

K

S

E

M

L

L

I

L

L

S

I

V

I

C

V

M

A

M

T

C

I

F

F

G

Q

S

M

L

S

V

A

I

P

A

S

C

H

L

W

P

D

G

G

Y

N

E

R

T

V

I

L

G

-

T

T

W

W

A

-

P

-

A

-

L

-

L

I

L

T

L

I

A

C

R

R

L

V

F

F

Q

L

G

G

L

I

S

G

V

I

G

G

E

D

Y

A

G

P

T

M

S

S

A

A

T

T

Y

V

M

V

S

S

E

D

V

R

A

A

V

N

E

I

G

H

R

R

K

R

G

G

F

T

Y

L

A

L

S

C

F

F

Q

I

Y
x
F

V

A

T

N

L

Q

I

G

G

W

G

G

Q

S

L

F

L

V

A

G

L

S

L

L

V

V

V

T

V

I

V

V

-

T

-

I

-

S

-

G

L

F

Q

K

H

H

T

R

M

L

E

K

D

S

A

G

A

H

L

T

R

H

-

D

-

V

E

D

W

K

G

A

W

W

R

R

I

I

P

L

F

I

A

G

L

L

G

S

A

L

V

I

L

P

A

A

V

F

V

G

A

T

L

L

W

Y

L

Q

R

R

binding phosphate ion: F145 (≠ Y166)

Sites not aligning to the query:

binding phosphate ion: 235, 239, 243, 346

Q9Z2I6 Synaptic vesicle glycoprotein 2C; Synaptic vesicle protein 2C from Rattus norvegicus (Rat) (see 3 papers)
29% identity, 31% coverage: 78:212/432 of query aligns to 203:337/727 of Q9Z2I6

query
sites
Q9Z2I6

I

V

G

G

G

A

W

F

L

F

F

W

G

G

R

G

I

L

A

A

D

D

K

K

H

V

G

G

R

R

K

K

K

Q

S

S

M

L

L

L

L

I

S

C

V

M

C

S

M

V

M

N

C

G

F

F

G

F

S

A

L

F

V

L

I

S

A

S

C

F

L

V

P

Q

G

G

Y

Y

E

-

T

-

I

-

G

-

T

-

W

-

A

-

P

-

A

G

L

F

L

L

A

C

R

R

L

L

F

L

Q

S

G

G

L

F

S

G

V

I

G

G

E

A

Y

I

G

P

T

T

S

V

A

F

T

S

Y

Y

M

F

S

A

E

E

V

V

A

L

V

A

E

R

G

E

R

K

K

R

G

G

F

E

Y

H

A

L

S

S

F

W

Q

L

Y

C

V

M

T

F

-

W

L

M

I

I

G

G

Q

I

L

Y

L

A

A

S

L

A

L

M

V

A

V

W

V

A

V

I

L

I

Q

P

H

H

-

Y

-

G

-

W

-

S

-

F

T

S

M

M

E

-

D

G

A

S

A

A

L

Y

R

Q

E

F

W

H

G

S

W

W

R

R

-

V

-

F

-

V

I

I

P

V

F

C

A

A

L

L

G

P

A

C

V

V

L

S

A

S

V

V

A

A

L

L

Sites not aligning to the query:

1:57 Interaction with SYT1
529:566 (Microbial infection) C.botulinum neurotoxin type A-binding
559 N→A: Loss of one glycosylation site. No effect on C.botulinum neurotoxin type A (BoNT/A, botA) binding, but reduces the uptake of BoNT/A.

Query Sequence

>16686 FitnessBrowser__Keio:16686
MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSGNTTT
QLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCMMCFGSLVIACLPGYETIG
TWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGFYASFQYVTLIGGQLLALLVV
VVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQLDETSQQETRALKEAGSLKGLWR
NRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGMHANVASGIMTAALFVFMLIQPLIG
ALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVSSPYAAFGLVMCALLIVSFYTSISGIL
KAEMFPAQVRALGVGLSYAVANAIFGGSAEYVALSLKSIGMETAFFWYVTLMAVVAFLVS
LMLHRKGKGMRL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory