SitesBLAST – Find functional sites

 

SitesBLAST

Comparing 16695 FitnessBrowser__Keio:16695 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

2pv7B Crystal structure of chorismate mutase / prephenate dehydrogenase (tyra) (1574749) from haemophilus influenzae rd at 2.00 a resolution (see paper)
57% identity, 75% coverage: 90:368/373 of query aligns to 1:280/280 of 2pv7B

query
sites
2pv7B
G
 
G
F
 
F
K
 
K
T
 
T
L
 
I
C
 
N
P
 
S
S
 
D
L
 
I
R
 
H
P
 
K
V
 
I
V
 
V
I
 
I
V
 
V
G
|
G
G
 
G
G
 
Y
G
|
G
Q
x
K
M
x
L
G
 
G
R
 
G
L
 
L
F
 
F
E
 
A
K
 
R
M
 
Y
L
 
L
T
 
R
L
 
A
S
 
S
G
 
G
Y
 
Y
Q
 
P
V
 
I
R
 
S
I
 
I
L
 
L
E
x
D
Q
x
R
H
 
E
D
 
D
W
 
W
D
 
A
R
 
V
A
 
A
A
 
E
D
 
S
I
 
I
V
 
L
A
 
A
D
 
N
A
 
A
G
 
D
M
 
V
V
 
V
I
 
I
V
 
V
S
 
S
V
|
V
P
|
P
I
|
I
H
 
N
V
 
L
T
 
T
E
 
L
Q
 
E
V
 
T
I
 
I
G
 
E
K
 
R
L
 
L
P
 
K
P
 
P
-
 
Y
L
 
L
P
 
T
K
 
E
D
 
N
C
 
M
I
 
L
L
 
L
V
 
A
D
 
D
L
|
L
A
x
T
S
|
S
V
 
V
K
 
K
N
 
R
G
 
E
P
 
P
L
 
L
Q
 
A
A
 
K
M
 
M
L
 
L
V
 
E
A
 
V
H
 
H
D
 
T
G
 
G
P
 
A
V
 
V
L
 
L
G
 
G
L
 
L
H
|
H
P
 
P
M
 
M
F
|
F
G
 
G
P
 
A
D
 
D
S
 
I
G
 
A
S
 
S
L
 
M
A
 
A
K
 
K
Q
 
Q
V
 
V
V
 
V
V
 
V
W
 
R
C
 
C
D
 
D
G
 
G
R
 
R
K
 
F
P
 
P
E
 
E
A
 
R
Y
 
Y
Q
 
E
W
 
W
F
 
L
L
 
L
E
 
E
Q
 
Q
I
 
I
Q
 
Q
V
 
I
W
 
W
G
 
G
A
 
A
R
 
K
L
 
I
H
 
Y
R
 
Q
I
 
T
S
 
N
A
 
A
V
 
T
E
 
E
H
 
H
D
 
D
Q
 
H
N
 
N
M
 
M
A
 
T
F
 
Y
I
 
I
Q
|
Q
A
 
A
L
 
L
R
 
R
H
 
H
F
 
F
A
 
S
T
 
T
F
 
F
A
 
A
Y
 
N
G
 
G
L
 
L
H
 
H
L
 
L
A
 
S
E
 
K
E
 
Q
N
 
P
V
 
I
Q
 
N
L
 
L
E
 
A
Q
 
N
L
 
L
L
 
L
A
 
A
L
 
L
S
 
S
S
 
S
P
 
P
I
 
I
Y
 
Y
R
 
R
L
 
L
E
 
E
L
 
L
A
 
A
M
 
M
V
 
I
G
 
G
R
|
R
L
 
L
F
 
F
A
 
A
Q
|
Q
D
 
D
P
 
A
Q
 
E
L
|
L
Y
|
Y
A
 
A
D
 
D
I
 
I
I
 
I
M
 
M
S
 
D
S
 
K
E
 
S
R
 
E
N
 
N
L
 
L
A
 
A
L
 
V
I
 
I
K
 
E
R
 
T
Y
 
L
Y
 
K
K
 
Q
R
 
T
F
 
Y
G
 
D
E
 
E
A
 
A
I
 
L
E
 
T
L
 
F
L
 
F
E
 
E
Q
 
N
G
 
N
D
 
D
K
 
R
Q
 
Q
A
 
G
F
 
F
I
 
I
D
 
D
S
 
A
F
 
F
R
 
H
K
 
K
V
 
V
E
 
R
H
 
D
W
 
W
F
 
F
G
 
G
D
 
D
Y
 
Y
A
 
S
Q
 
E
R
 
Q
F
 
F
Q
 
L
S
 
K
E
 
E
S
 
S
R
 
R
V
 
Q
L
 
L
L
 
L
R
 
Q
Q
 
Q
A
 
A

5t9fB Prephenate dehydrogenase n222d mutant from soybean (see paper)
23% identity, 52% coverage: 106:299/373 of query aligns to 9:227/253 of 5t9fB

query
sites
5t9fB
G
|
G
G
 
F
G
|
G
Q
x
N
M
x
F
G
 
G
R
 
Q
L
 
F
F
 
L
E
 
A
K
 
K
M
 
T
L
 
M
T
 
I
L
 
K
S
 
Q
G
 
G
Y
 
H
Q
 
T
V
 
L
R
 
T
I
 
A
L
 
T
E
x
S
Q
x
R
H
x
S
D
 
D
W
x
Y
-
 
S
-
 
E
-
 
L
-
 
C
-
 
L
-
 
Q
-
 
M
-
 
G
-
 
I
-
 
H
-
 
F
-
 
F
-
 
R
D
 
D
R
 
V
A
 
S
A
 
A
D
 
F
I
 
L
V
 
T
A
 
A
D
 
D
A
 
I
G
 
D
M
 
V
V
 
I
I
 
V
V
 
L
S
x
C
V
x
T
P
x
S
I
|
I
H
 
L
V
x
S
T
 
L
E
 
S
Q
 
E
V
 
V
I
 
V
G
 
G
K
 
S
L
 
M
P
 
P
-
 
L
-
 
T
P
 
S
L
 
L
P
 
K
K
 
R
D
 
P
C
 
T
I
 
L
L
 
F
V
 
V
D
 
D
L
x
V
A
x
L
S
|
S
V
 
V
K
 
K
N
 
E
G
 
H
P
 
P
L
 
R
Q
 
E
A
 
L
M
 
L
L
 
L
-
 
R
-
 
E
V
 
L
A
 
P
H
 
E
D
 
D
G
 
S
P
 
D
V
 
I
L
 
L
G
 
C
L
 
T
H
|
H
P
 
P
M
 
M
F
 
F
G
|
G
P
|
P
D
x
Q
S
x
T
G
 
A
S
 
K
L
 
N
A
 
G
K
 
-
Q
 
-
V
 
-
V
 
-
V
 
-
W
 
W
C
 
T
D
 
D
G
 
H
-
 
T
-
 
F
-
 
M
-
 
Y
-
 
D
-
 
K
-
 
V
-
 
R
-
 
I
R
 
R
K
 
D
P
 
E
E
 
V
A
 
I
Y
 
C
Q
 
S
W
 
N
F
 
F
L
 
I
E
 
Q
Q
 
I
I
 
F
Q
 
A
V
 
T
W
 
E
G
 
G
A
 
C
R
 
K
L
 
M
H
 
V
R
 
Q
I
 
M
S
 
S
A
 
C
V
 
E
E
 
E
H
 
H
D
 
D
Q
 
R
N
 
A
M
 
A
A
 
A
F
 
K
I
 
S
Q
|
Q
A
 
-
L
 
-
R
 
-
H
 
-
F
 
F
A
 
I
T
 
T
F
 
H
A
 
T
Y
 
I
G
 
G
L
 
R
H
 
T
L
 
L
A
 
G
E
 
E
E
 
M
N
 
D
V
 
I
Q
 
Q
-
 
S
-
 
T
-
 
P
-
 
I
-
 
D
-
 
T
-
 
K
-
 
G
L
 
F
E
 
E
Q
 
T
L
 
L
L
 
V
A
 
K
L
 
L
S
 
K
S
 
E
P
 
T
I
 
M
Y
 
M
R
 
R
L
x
D
E
x
S
L
 
F
A
 
D
M
 
L
V
 
Y
G
 
S
R
 
G
L
 
L
F
 
F
A
 
V
Q
 
Y
D
 
N

2gtvX Nmr structure of monomeric chorismate mutase from methanococcus jannaschii (see paper)
38% identity, 13% coverage: 1:50/373 of query aligns to 1:58/104 of 2gtvX

query
sites
2gtvX
M
 
M
V
 
I
A
 
E
E
 
K
L
 
L
T
 
A
A
 
E
L
 
I
R
|
R
D
 
K
Q
 
K
I
 
I
D
 
D
E
 
E
V
 
I
D
 
D
K
 
N
A
 
K
L
 
I
L
 
L
N
 
K
-
 
A
-
 
R
-
 
W
-
 
P
-
 
W
-
 
A
-
 
E
-
 
K
L
 
L
L
 
I
A
 
A
K
 
E
R
|
R
L
 
N
E
 
S
L
 
L
V
 
A
A
 
K
E
 
D
V
|
V
G
 
A
E
 
E
V
 
I
K
|
K
S
 
N
R
 
Q
F
 
L
G
 
G
L
 
I
P
 
P
I
 
I
Y
 
N
V
x
D
P
 
P
E
 
E
R
|
R
E
|
E

Sites not aligning to the query:

Query Sequence

>16695 FitnessBrowser__Keio:16695
MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPIYVPEREASMLASRRAE
AEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVVIVGGGGQMGRLFEKMLTL
SGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIGKLPPLPKDCILVDLASVKNG
PLQAMLVAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGRKPEAYQWFLEQIQVWGARLHRI
SAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQLLALSSPIYRLELAMVGRLFAQDP
QLYADIIMSSERNLALIKRYYKRFGEAIELLEQGDKQAFIDSFRKVEHWFGDYAQRFQSE
SRVLLRQANDNRQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory