SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 16751 FitnessBrowser__Keio:16751 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
100% identity, 100% coverage: 1:482/482 of query aligns to 1:482/482 of P25526

query
sites
P25526

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WN 156:157 (= WN 156:157) binding NADP(+)
KPAS 180:183 (= KPAS 180:183) binding NADP(+)
GS 233:234 (= GS 233:234) binding NADP(+)
L256 (= L256) binding NADP(+)
E386 (= E386) binding NADP(+)

3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
100% identity, 100% coverage: 2:482/482 of query aligns to 1:481/481 of 3jz4A

query
sites
3jz4A

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active site: N156 (= N157), K179 (= K180), E254 (= E255), C288 (= C289), E385 (= E386), E462 (= E463)
binding nadp nicotinamide-adenine-dinucleotide phosphate: P154 (= P155), W155 (= W156), K179 (= K180), A181 (= A182), S182 (= S183), A212 (= A213), G216 (= G217), G232 (= G233), S233 (= S234), I236 (= I237), C288 (= C289), K338 (= K339), E385 (= E386), F387 (= F388)

8c54A Cryo-em structure of nadh bound sla dehydrogenase rlgabd from rhizobium leguminosarum bv. Trifolii srd1565 (see paper)
62% identity, 99% coverage: 3:481/482 of query aligns to 2:480/482 of 8c54A

query
sites
8c54A

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A

D

D

A

A

L

M

E

A

K

N

G

G

A

G

R

T

V

V

V

L

C

T

G

G

K

K

A

R

H

S

E

V

R

L

G

G

N

T

F

F

Q

E

P

P

T

T

I

V

L

V

V

T

D

G

V

V

P

T

A

Q

N

T

A

M

K

K

V

V

S

A

K

K

E

E

T

T

F

F

G

A

P

P

L

L

A

A

P

P

L

I

F

I

R

S

F

F

K

K

D

H

E

E

A

N

D

D

V

V

I

I

A

A

Q

M

A

A

N

N

D

D

T

S

E

E

F

F

G

G

L

L

A

A

A

S

Y

Y

F

F

Y

Y

A

A

R

K

D

D

L

M

S

A

R

R

V

I

F

W

R

R

V

V

G

A

E

E

A

A

L

L

E

E

Y

A

G

G

I

M

V

V

G

G

I

V

N

N

T

T

G

G

I

M

I

I

S

A

N

N

E

E

V

M

A

A

P

P

F

F

G

G

I

I

K

K

A

Q

S

S

G

G

L

T

G

G

R

R

E

E

G

G

S

S

K

K

Y

Y

G

G

I

I

E

E

D

G

Y

F

L

L

E

E

I

L

K

K

Y

Y

M

V

C

A

I

L

G

G

binding 1,4-dihydronicotinamide adenine dinucleotide: I152 (= I153), T153 (= T154), P154 (= P155), K179 (= K180), A212 (= A213), K213 (≠ G214), F230 (= F231), T231 (= T232), G232 (= G233), S233 (= S234), V236 (≠ I237), W239 (≠ Q240), G256 (= G257)

8of1A Structure of aldh5f1 from moss physcomitrium patens in complex with NAD+ in the contracted conformation
57% identity, 100% coverage: 1:481/482 of query aligns to 18:498/505 of 8of1A

query
sites
8of1A

M

V

K

Q

L

L

N

N

D

E

S

A

N

G

L

L

F

F

R

K

Q

S

Q

Q

A

G

L

L

I

I

N

G

G

D

E

K

W

W

L

V

D

D

A

A

N

E

N

N

G

G

E

H

A

T

I

L

D

P

V

V

T

N

N

N

P

P

A

A

N

T

G

G

D

E

K

I

L

L

G

T

S

S

V

V

P

P

K

F

M

M

G

G

A

K

D

R

E

E

T

A

R

E

A

K

A

A

I

I

D

A

A

A

N

S

R

Q

A

A

L

F

P

T

A

S

W

W

R

S

A

K

L

R

T

T

A

A

K

N

E

D

R

R

A

S

T

K

I

I

L

L

R

R

N

Q

W

W

F

F

N

N

L

L

M

L

M

I

E

K

H

N

Q

K

D

D

L

L

A

G

R

K

L

L

M

I

T

V

L

L

E

E

Q

Q

G

G

K

K

P

P

L

L

A

A

E

E

A

A

K

V

G

G

E

E

I

I

S

V

Y

Y

A

G

A

A

S

A

F

F

I

V

E

E

W

Y

F

Y

A

A

E

E

G

A

K

K

R

R

I

V

Y

Y

G

G

D

D

T

I

I

I

P

P

G

S

H

P

Q

F

A

P

D

E

K

K

R

R

L

M

I

L

V

V

I

M

K

K

Q

Q

P

P

I

V

G

G

V

V

T

V

A

A

I
|
I

T
x
A

P
|
P

W
|
W

N

N

F

F

P

P

A

L

A

A

M

M

I

I

T

T

R

R

K

K

A

V

G

A

P

P

A

A

L

L

A

A

G

G

C

C

T

T

M

V

V

V

L

I

K
|
K

P

P

A

S

S

E

Q

L

T

T

P

P

F

L

S

T

A

A

L

L

A

A

L

A

A

A

E

E

L

L

A

A

I

L

R

Q

A

A

G

G

V

I

P

P

A

P

G

G

V

V

F

V

N

N

V

V

T

M

G

G

S

D

A
|
A

G

K

A

G

V

I

G

G

N

D

E

A

L

M

T

L

S

D

N

S

P

T

L

E

V

V

R

R

K

K

L

I

S

T

F
|
F

T

T

G
|
G

S
|
S

T

T

E

G

I
x
V

G

G

R

K

Q
x
M

L

L

M

L

E

A

Q

G

C

A

A

G

K

K

D

T

I

V

K

K

V

V

S

S

L

L

E

E

L
|
L

G

G

N

N

A

A

P

P

F

C

I

I

V

V

F

F

D

D

A

A

D

N

L

L

D

D

K

V

A

A

V

V

E

K

G

G

A

V

L

L

A

A

S

G

K

K

F

Y

R

R

N

N

A

S

G

G

Q

Q

T

T

C
|
C

V

V

C

C

A

I

N

N

R

K

L

I

Y

F

V

V

Q

Q

D

D

G

G

V

I

Y

Y

D

D

R

K

F

F

A

A

E

E

K

A

L

F

Q

A

Q

K

A

A

V

V

S

S

K

G

L

L

H

R

I

A

G

G

D

N

G

G

L

L

D

E

N

P

G

G

V

I

T

T

I

Q

G

G

P

P

L

L

I

I

D

N

E

E

K

T

A

A

V

L

A

E

K
|
K

V

V

E

E

E

R

H

H

I

V

A

Q

D

D

A

A

L

V

E

S

K

K

G

G

A

A

R

K

V

V

V

L

C

V

G

G

K

K

A

R

H

H

E

S

R

L

G

G

G

R

N

T

F

F

F

Y

Q

E

P

P

T

T

I

I

L

L

V

G

D

N

V

A

P

S

A

D

N

E

A

M

K

L

V

I

S

F

K

R

E

E

E
|
E

T

V

F
|
F

G

G

P

P

L

V

A

A

P

P

L

L

F

V

R

R

F

F

K

N

D

T

E

D

A

E

D

E

V

A

I

I

A

K

Q

L

A

A

N

N

D

N

T

S

E

E

F

F

G

G

L

L

A

A

Y

Y

F

A

Y

F

A

T

R

E

D

N

L

I

S

T

R

R

V

G

F

W

R

R

V

V

G

A

E

E

A

S

L

L

E

E

Y

F

G

G

I

M

V

V

G

G

I

L

N

N

T

E

G

G

I

L

I

I

S

S

N

T

E

E

V

V

A

A

P

P

F

F

G

G

I

M

K

K

A

Q

S

S

G

G

L

L

G

G

R

R

E

E

G

G

S

S

K

K

Y

Y

G

G

I

L

E

D

D

E

Y

Y

L

L

E

E

I

M

K

K

Y

Y

M

V

C

C

I

L

G

G

binding nicotinamide-adenine-dinucleotide: I170 (= I153), A171 (≠ T154), P172 (= P155), W173 (= W156), K197 (= K180), A230 (= A213), F248 (= F231), G250 (= G233), S251 (= S234), V254 (≠ I237), M257 (≠ Q240), L273 (= L256), C306 (= C289), K356 (= K339), E403 (= E386), F405 (= F388)

P51649 Succinate-semialdehyde dehydrogenase, mitochondrial; Aldehyde dehydrogenase family 5 member A1; NAD(+)-dependent succinic semialdehyde dehydrogenase; EC 1.2.1.24 from Homo sapiens (Human) (see 5 papers)
56% identity, 98% coverage: 8:481/482 of query aligns to 58:533/535 of P51649

query
sites
P51649

L

L

F

L

R

R

Q

T

Q

D

A

S

L

F

I

V

N

G

G

G

E

R

W

W

L

L

D

P

A

A

N

-

G

A

E

A

A

T

I

F

D

P

V

V

T

Q

N

D

P

P

A

A

N

S

G

G

D

A

K

A

L

L

G

G

S

M

V

V

P

A

K

D

M
x
C

G

G

A

V

D

R

E

E

T

A

R

R

A

A

I

V

D

R

A

A

N

Y

R

E

A

A

L

F

P

C

A

R

W

W

R

R

A

E

L

V

T

S

A

A

K

K

E

E

R

R

A

S

T

S

I

L

L

L

R

R

N

K

W

W

F

Y

N

N

L

L

M

M

M

I

E

Q

H

N

Q

K

D

D

L

L

A

A

R

R

L

I

M

I

T

T

L

A

E

E

Q

S

G

G

K

K

P

P

L

L

A

K

E

E

A

A

K

H

G

G

E

E

I

I

S

L

Y

Y

A

S

A

A

S

F

F

F

I

L

E

E

W

W

F

F

A

S

E

E

G

A

K

R

R

R

I

V

Y

Y

G
|
G

D

D

T

I

I

I

P
x
H

G

T

H
x
P

Q

A

A

K

D

D

K

R

R

R

L

A

I

L

V

V

I

L

K

K

Q

Q

P

P

I

I

G

G

V

V

T

A

A

A

A

V

I

I

T

T

P

P

W

W

N

N

F

F

P

P

A

S

A

A

M

M

I

I

T

T

R
|
R

K

K

A

V

G

G

P

A

A

A

L

L

A

A

G

G

C
|
C

T

T

M

V

V

V

L

V

K
|
K

P
|
P

A
|
A

S
x
E

Q

D

T
|
T

P

P

F

F

S

S

A
|
A

L

L

A

A

L

L

A

A

E

E

L

L

A

A

I

S

R

Q

A

A

G

G

V

I

P

P

A

S

G

G

V

V

F

Y

N
|
N

V

V

V

I

T

P

G

C

S

S

-

R

-

K

-

N

A

A

G

K

A

E

V

V

G
|
G

N

E

E

A

L

I

T

C

S

T

N

D

P

P

L

L

V

V

R

S

K

K

L

I

S

S

F

F

T

T

G
|
G

S
|
S

T
|
T

E
x
T

I
x
T

G
|
G

R

K

Q

I

L

L

M

L

E

H

Q

H

C

A

A

A

K

N

D

S

I

V

K

K

K

R

V

V

S

S

L

M

E

E

L

L

G

G

N

L

A

A

P

P

F

F

I

I

V

V

F

F

D

D

D

S

A

A

D

N

L

V

D

D

K

Q

A

A

V

V

E

A

G

G

A

A

L

M

A

A

S

S

K

K

F

F

R
|
R

N
|
N

A

T

G

G

Q

Q

T

T

C
|
C

V

V

C
|
C

A

S

N

N

R

Q

L

F

Y

L

V

V

Q

Q

D

R

G

G

V

I

Y

H

D

D

R

A

F

F

A

V

E

K

K

A

L

F

Q

A

Q

E

A

A

V

M

S

K

K

K

-

N

L

L

H

R

I

V

G

G

D
x
N

G

G

L

F

D

E

N

E

G

G

V

T

T

T

I

Q

G

G

P
|
P

L

L

I

I

D

N

E

E

K

K

A

A

V

V

A

E

K

K

V

V

E

E

E

K

H

Q

I

V

A

N

D

D

A

A

L

V

E

S

K

K

G

G

A

A

R

T

V
|
V

V

V

C

T

G
|
G

G

G

K

K

A

R

H

H

E

Q

R

L

G

G

G

K

N

N

F

F

Q

E

P

P

T

T

I

L

L

L

V

C

D

N

V

V

P

T

A

Q

N

D

A

M

K

L

V

C

S

T

K

H

E

E

T

T

F

F

G

G

P

P

L

L

A

A

P

P

L

V

F

I

R

K

F

F

K

D

D

T

E

E

A

E

D

E

V

A

I

I

A

A

Q

I

A

A

N

N

D

A

T

A

E

D

F

V

G

G

L

L

A

A

A

G

Y

Y

F

F

Y

Y

A

S

R

Q

D

D

L

P

S

A

R

Q

V

I

F

W

R

R

V

V

G

A

E

E

A

Q

L

L

E

E

Y

V

G

G

I

M

V

V

G

G

I

V

N

N

T

E

G

G

I

L

I

I

S
|
S

N

S

E

V

V

E

A

C

P

P

F

F

G

G

I

V

K

K

A

Q

S

S

G

G

L

L

G

G

R

R

E

E

G

G

S

S

K

K

Y

Y

G

G

I

I

E

D

D

E

Y

Y

L

L

E

E

I

L

K

K

Y

Y

M

V

C

C

I

Y

G
|
G

C93 (≠ M45) to F: in SSADHD; 3% of activity; dbSNP:rs765561257
G176 (= G128) to R: in SSADHD; <1% of activity; dbSNP:rs72552281
H180 (≠ P132) to Y: 83% of activity; dbSNP:rs2760118
P182 (≠ H134) to L: 48% of activity; dbSNP:rs3765310
R213 (= R165) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
C223 (= C175) to Y: in SSADHD; 5% of activity; dbSNP:rs72552282
KPAE 228:231 (≠ KPAS 180:183) binding NAD(+)
T233 (= T185) to M: in SSADHD; 4% of activity; dbSNP:rs1326526453
A237 (= A189) to S: 65% of activity; dbSNP:rs62621664
N255 (= N207) to S: in SSADHD; 17% of activity; dbSNP:rs145087265
G268 (= G217) to E: in SSADHD; <1% of activity; dbSNP:rs375628463
GSTTTG 284:289 (≠ GSTEIG 233:238) binding NAD(+)
R334 (= R283) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
N335 (= N284) to K: in SSADHD; 1% of activity; dbSNP:rs72552283
C340 (= C289) modified: Disulfide link with 342, In inhibited form
C342 (= C291) modified: Disulfide link with 340, In inhibited form; mutation to A: Loss of regulation by redox state.
N372 (≠ D320) natural variant: N -> S
P382 (= P330) to L: in SSADHD; 2% of activity
V406 (= V354) to I: in dbSNP:rs143741652
G409 (= G357) to D: in SSADHD; <1% of activity; dbSNP:rs118203984
S498 (= S446) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
G533 (= G481) to R: in SSADHD; <1% of activity; dbSNP:rs72552284

Sites not aligning to the query:

36 G → R: no effect on succinate-semialdehyde dehydrogenase activity; dbSNP:rs4646832

2w8rA The crystal structure of human ssadh in complex with NAD+ (see paper)
55% identity, 98% coverage: 8:481/482 of query aligns to 8:483/485 of 2w8rA

query
sites
2w8rA

L

L

F

L

R

R

Q

T

Q

D

A

S

L

F

I

V

N

G

G

G

E

R

W

W

L

L

D

P

A

A

N

-

G

A

E

A

A

T

I

F

D

P

V

V

T

Q

N

D

P

P

A

A

N

S

G

G

D

A

K

A

L

L

G

G

S

M

V

V

P

A

K

D

M

C

G

G

A

V

D

R

E

E

T

A

R

R

A

A

I

V

D

R

A

A

N

Y

R

E

A

A

L

F

P

C

A

R

W

W

R

R

A

E

L

V

T

S

A

A

K

K

E

E

R

R

A

S

T

S

I

L

L

L

R

R

N

K

W

W

F

Y

N

N

L

L

M

M

M

I

E

Q

H

N

Q

K

D

D

L

L

A

A

R

R

L

I

M

I

T

T

L

A

E

E

Q

S

G

G

K

K

P

P

L

L

A

K

E

E

A

A

K

H

G

G

E

E

I

I

S

L

Y

Y

A

S

A

A

S

F

F

F

I

L

E

E

W

W

F

F

A

S

E

E

G

A

K

R

R

R

I

V

Y

Y

G

G

D

D

T

I

I

I

P

H

G

T

H

P

Q

A

A

K

D

D

K

R

R

R

L

A

I

L

V

V

I

L

K

K

Q

Q

P

P

I

I

G

G

V

V

T

A

A

A

A

V

I

I

T
|
T

P

P

W

W

N
|
N

F

F

P

P

A

S

A

A

M

M

I

I

T

T

R

R

K

K

A

V

G

G

P

A

A

A

L

L

A

A

G

G

C

C

T

T

M

V

V

V

L

V

K
|
K

P

P

A

A

S

E

Q

D

T

T

P

P

F

F

S

S

A

A

L

L

A

A

L

L

A

A

E

E

L

L

A

A

I

S

R

Q

A

A

G

G

V

I

P

P

A

S

G

G

V

V

F

Y

N

N

V

V

V

I

T

P

G

C

S

S

-

R

-

K

-

N

A
|
A

G

K

A

E

V

V

G

G

N

E

E

A

L

I

T

C

S

T

N

D

P

P

L

L

V

V

R

S

K

K

L

I

S

S

F

F

T

T

G

G

S
|
S

T

T

E

T

I
x
T

G

G

R

K

Q

I

L

L

M

L

E

H

Q

H

C

A

A

A

K

N

D

S

I

V

K

K

K

R

V

V

S

S

L

M

E
|
E

L

L

G

G

N

L

A

A

P

P

F

F

I

I

V

V

F

F

D

D

D

S

A

A

D

N

L

V

D

D

K

Q

A

A

V

V

E

A

G

G

A

A

L

M

A

A

S

S

K

K

F

F

R

R

N

N

A

T

G

G

Q

Q

T

T

C
x
A

V

V

C

C

A

S

N

N

R

Q

L

F

Y

L

V

V

Q

Q

D

R

G

G

V

I

Y

H

D

D

R

A

F

F

A

V

E

K

K

A

L

F

Q

A

Q

E

A

A

V

M

S

K

K

K

-

N

L

L

H

R

I

V

G

G

D

N

G

G

L

F

D

E

N

E

G

G

V

T

T

T

I

Q

G

G

P

P

L

L

I

I

D

N

E

E

K

K

A

A

V

V

A

E

K

K

V

V

E

E

E

K

H

Q

I

V

A

N

D

D

A

A

L

V

E

S

K

K

G

G

A

A

R

T

V

V

C

T

G

G

K

K

A

R

H

H

E

Q

R

L

G

G

G

K

N

N

F

F

Q

E

P

P

T

T

I

L

L

L

V

C

D

N

V

V

P

T

A

Q

N

D

A

M

K

L

V

C

S

T

K

H

E

E

E
|
E

T

T

F

F

G

G

P

P

L

L

A

A

P

P

L

V

F

I

R

K

F

F

K

D

D

T

E

E

A

E

D

E

V

A

I

I

A

A

Q

I

A

A

N

N

D

A

T

A

E

D

F

V

G

G

L

L

A

A

A

G

Y

Y

F

F

Y

Y

A

S

R

Q

D

D

L

P

S

A

R

Q

V

I

F

W

R

R

V

V

G

A

E

E

A

Q

L

L

E

E

Y

V

G

G

I

M

V

V

G

G

I

V

N

N

T

E

G

G

I

L

I

I

S

S

N

S

E

V

V

E

A

C

P

P

F

F

G

G

I

V

K

K

A

Q

S

S

G

G

L

L

G

G

R

R

E
|
E

G

G

S

S

K

K

Y

Y

G

G

I

I

E

D

D

E

Y

Y

L

L

E

E

I

L

K

K

Y

Y

M

V

C

C

I

Y

G

G

active site: N155 (= N157), K178 (= K180), E256 (= E255), A290 (≠ C289), E388 (= E386), E465 (= E463)
binding adenosine-5'-diphosphate: T152 (= T154), K178 (= K180), A214 (= A213), S235 (= S234), T238 (≠ I237)

2w8qA The crystal structure of human ssadh in complex with ssa. (see paper)
55% identity, 98% coverage: 8:481/482 of query aligns to 8:483/485 of 2w8qA

query
sites
2w8qA

L

L

F

L

R

R

Q

T

Q

D

A

S

L

F

I

V

N

G

G

G

E

R

W

W

L

L

D

P

A

A

N

-

G

A

E

A

A

T

I

F

D

P

V

V

T

Q

N

D

P

P

A

A

N

S

G

G

D

A

K

A

L

L

G

G

S

M

V

V

P

A

K

D

M

C

G

G

A

V

D

R

E

E

T

A

R

R

A

A

I

V

D

R

A

A

N

Y

R

E

A

A

L

F

P

C

A

R

W

W

R

R

A

E

L

V

T

S

A

A

K

K

E

E

R

R

A

S

T

S

I

L

L

L

R

R

N

K

W

W

F

Y

N

N

L

L

M

M

M

I

E

Q

H

N

Q

K

D

D

L

L

A

A

R

R

L

I

M

I

T

T

L

A

E

E

Q

S

G

G

K

K

P

P

L

L

A

K

E

E

A

A

K

H

G

G

E

E

I

I

S

L

Y

Y

A

S

A

A

S

F

F

F

I

L

E

E

W

W

F

F

A

S

E

E

G

A

K

R

R

R

I

V

Y

Y

G

G

D

D

T

I

I

I

P

H

G

T

H

P

Q

A

A

K

D

D

K

R

R

R

L

A

I

L

V

V

I

L

K

K

Q

Q

P

P

I

I

G

G

V

V

T

A

A

A

A

V

I

I

T

T

P

P

W

W

N
|
N

F

F

P

P

A

S

A

A

M

M

I

I

T

T

R
|
R

K

K

A

V

G

G

P

A

A

A

L

L

A

A

G

G

C

C

T

T

M

V

V

V

L

V

K
|
K

P

P

A

A

S

E

Q

D

T

T

P

P

F

F

S

S

A

A

L

L

A

A

L

L

A

A

E

E

L

L

A

A

I

S

R

Q

A

A

G

G

V

I

P

P

A

S

G

G

V

V

F

Y

N

N

V

V

V

I

T

P

G

C

S

S

-

R

-

K

-

N

A

A

G

K

A

E

V

V

G

G

N

E

E

A

L

I

T

C

S

T

N

D

P

P

L

L

V

V

R

S

K

K

L

I

S

S

F

F

T

T

G

G

S

S

T

T

E

T

I

T

G

G

R

K

Q

I

L

L

M

L

E

H

Q

H

C

A

A

A

K

N

D

S

I

V

K

K

K

R

V

V

S

S

L

M

E
|
E

L

L

G

G

N

L

A

A

P

P

F

F

I

I

V

V

F

F

D

D

D

S

A

A

D

N

L

V

D

D

K

Q

A

A

V

V

E

A

G

G

A

A

L

M

A

A

S

S

K

K

F

F

R
|
R

N

N

A

T

G

G

Q

Q

T

T

C
x
A

V

V

C

C

A

S

N

N

R

Q

L

F

Y

L

V

V

Q

Q

D

R

G

G

V

I

Y

H

D

D

R

A

F

F

A

V

E

K

K

A

L

F

Q

A

Q

E

A

A

V

M

S

K

K

K

-

N

L

L

H

R

I

V

G

G

D

N

G

G

L

F

D

E

N

E

G

G

V

T

T

T

I

Q

G

G

P

P

L

L

I

I

D

N

E

E

K

K

A

A

V

V

A

E

K

K

V

V

E

E

E

K

H

Q

I

V

A

N

D

D

A

A

L

V

E

S

K

K

G

G

A

A

R

T

V

V

C

T

G

G

K

K

A

R

H

H

E

Q

R

L

G

G

G

K

N

N

F

F

Q

E

P

P

T

T

I

L

L

L

V

C

D

N

V

V

P

T

A

Q

N

D

A

M

K

L

V

C

S

T

K

H

E

E

E
|
E

T

T

F

F

G

G

P

P

L

L

A

A

P

P

L

V

F

I

R

K

F

F

K

D

D

T

E

E

A

E

D

E

V

A

I

I

A

A

Q

I

A

A

N

N

D

A

T

A

E

D

F

V

G

G

L

L

A

A

A

G

Y

Y

F

F

Y

Y

A

S

R

Q

D

D

L

P

S

A

R

Q

V

I

F

W

R

R

V

V

G

A

E

E

A

Q

L

L

E

E

Y

V

G

G

I

M

V

V

G

G

I

V

N

N

T

E

G

G

I

L

I

I

S
|
S

N

S

E

V

V

E

A

C

P

P

F

F

G

G

I

V

K

K

A

Q

S

S

G

G

L

L

G

G

R

R

E
|
E

G

G

S

S

K

K

Y

Y

G

G

I

I

E

D

D

E

Y

Y

L

L

E

E

I

L

K

K

Y

Y

M

V

C

C

I

Y

G

G

active site: N155 (= N157), K178 (= K180), E256 (= E255), A290 (≠ C289), E388 (= E386), E465 (= E463)
binding succinic acid: R163 (= R165), R284 (= R283), A290 (≠ C289), S448 (= S446)

5x5uA Crystal structure of alpha-ketoglutarate-semialdehyde dehydrogenase (kgsadh) complexed with NAD (see paper)
41% identity, 97% coverage: 12:480/482 of query aligns to 6:474/476 of 5x5uA

query
sites
5x5uA

Q

Q

A

L

L

L

I

I

N

D

G

G

E

E

W

W

L

V

D
|
D

A
|
A

N
x
A

N

S

G
|
G

E

K

A

T

I

I

D

D

V

V

T

V

N

N

P

P

A

A

N

T

G

G

D

K

K

P

L

I

G

G

S

R

V

V

P

A

K

H

M

A

G

G

A

I

D

A

E

D

T

L

R

D

A

R

A

A

I

L

D

A

A

A

N

Q

R

S

A

G

L

F

P

E

A

A

W

W

R

R

A

K

L

V

T

P

A

A

K

H

E

E

R

R

A

A

T

A

I

T

L

M

R

R

N

K

W

A

F

A

N

A

L

L

M

V

M

R

E

E

H

R

Q

A

D

D

D

A

L

I

A

A

R

Q

L

L

M

M

T

T

L

Q

E

E

Q

Q

G

G

K

K

P

P

L

L

A

T

E

E

A

A

K

R

G

V

E

E

I

V

S

L

Y

S

A

A

S

D

F

I

I

I

E

E

W

W

F

F

A

A

E

D

E

E

G

G

K

R

R

R

I

V

Y

Y

G

G

D

R

T

I

I

V

P

P

G

P

H

R

Q

N

A

L

D

G

K

A

R

Q

L

Q

I

T

V

V

I

V

K

K

Q

E

P

P

I

V

G

G

V

P

T

V

A

A

I

F

T

T

P
|
P

W

W

N
|
N

F

F

P

P

A

V

A

N

M

Q

I

V

T

V

R

R

K

K

A

L

G

S

P

A

A

A

L

L

A

A

A

T

G

G

C

C

T

S

M

F

V

L

L

V

K
|
K

P

A

A

P

S

E

Q

E

T

T

P

P

F

A

S

S

A

P

L

A

A

A

L

L

A

L

E

R

L

A

A

F

I

V

R

D

A

A

G

G

V

V

P

P

A

A

G

G

V

V

F

I

N

G

V

L

V

V

T

Y

G

G

S

D

A
x
P

G
x
A

A

E

V

I

G
x
S

N

S

E

Y

L

L

T

I

S

P

N

H

P

P

L

V

V

I

R

R

K

K

L

V

S

T

F

F

T

T

G
|
G

S
|
S

T

T

E

P

I
x
V

G

G

R

K

Q

Q

L

L

M

A

E

S

Q

L

C

A

A

G

K

L

D

H

I

M

K

K

K

R

V

A

S

T

L

M

E
|
E

L

L

G

G

N

H

A

A

P

P

F

V

I

I

V

V

F

A

D

E

D

D

A

A

D

D

L

V

D

A

K

L

A

A

V

V

E

K

G

A

A

A

L

G

A

G

S

A

K

K

F

F

R

R

N

N

A

A

G

G

Q

Q

T

V

C
|
C

V

I

C

S

A

P

N

T

R

R

L

F

Y

L

V

V

Q

H

D

N

G

S

V

I

Y

R

D

D

R

E

F

F

A

T

E

R

K

A

L

L

Q

V

Q

K

A

H

V

A

S

E

K

G

L

L

H

K

I

V

G

G

D

N

G

G

L

L

D

E

N

E

G

G

V

T

T

T

I

L

G

G

P

A

L

L

I

A

D

N

E

P

K
x
R

A
x
R

V

L

A

T

K

A

V

M

E

A

E

S

H

V

I

I

A

D

D

N

A

A

L

R

E

K

K

V

G

G

A

A

R

S

V

I

V

E

C

T

G

G

K

E

A

R

H

I

E

G

R

S

G

E

G

G

N

N

F

F

Q

A

P

P

T

T

I

V

L

I

V

A

D

N

V

V

P

P

A

L

N

D

A

A

K

D

V

V

S

F

K

N

E

N

E
|
E

T

P

F
|
F

G

G

P

P

L

V

A

A

P

A

L

I

F

R

R

G

F

F

K

D

D

K

E

L

A

E

D

E

V

A

I

I

A

A

Q

E

A

A

N

N

D

R

T

L

E

P

F

F

G

G

L

L

A

A

A

G

Y

Y

F

A

Y

F

A

T

R

R

D

S

L

F

S

A

R

N

V

V

F

H

R

L

V

L

G

T

E

Q

A

R

L

L

E

E

Y

V

G

G

I

M

V

L

G

W

I

I

N

N

T

Q

G

P

I

A

I

T

S

P

N

W

E

P

V

E

A

M

P

P

F

F

G

G

I

V

K

K

A

D

S

S

G

G

L

Y

G

G

R

S

E
|
E

G

G

S

G

K

P

Y

E

G

A

I

L

E

E

D

P

Y

Y

L

L

E

V

I

T

K

K

Y

S

M

V

C

T

I

V

active site: N151 (= N157), K174 (= K180), E249 (= E255), C283 (= C289), E380 (= E386), E457 (= E463)
binding glycerol: D15 (= D21), A16 (= A22), A17 (≠ N23), G19 (= G25)
binding nicotinamide-adenine-dinucleotide: P149 (= P155), P207 (≠ A213), A208 (≠ G214), S211 (≠ G217), G227 (= G233), S228 (= S234), V231 (≠ I237), R329 (≠ K335), R330 (≠ A336), E380 (= E386), F382 (= F388)

5x5tA Crystal structure of alpha-ketoglutarate semialdehyde dehydrogenase (kgsadh) from azospirillum brasilense (see paper)
41% identity, 97% coverage: 12:480/482 of query aligns to 6:474/476 of 5x5tA

query
sites
5x5tA

Q

Q

A

L

L

L

I

I

N

D

G

G

E

E

W

W

L

V

D

D

A

A

N

A

N

S

G

G

E

K

A

T

I

I

D

D

V

V

T

V

N

N

P

P

A

A

N

T

G

G

D

K

K

P

L

I

G

G

S

R

V

V

P

A

K

H

M

A

G

G

A

I

D

A

E

D

T

L

R

D

A

R

A

A

I

L

D

A

A

A

N

Q

R

S

A

G

L

F

P

E

A

A

W

W

R

R

A

K

L

V

T

P

A

A

K

H

E

E

R

R

A

A

T

A

I

T

L

M

R

R

N

K

W

A

F

A

N

A

L

L

M

V

M

R

E

E

H

R

Q

A

D

D

D

A

L

I

A

A

R

Q

L

L

M

M

T

T

L

Q

E

E

Q

Q

G

G

K

K

P

P

L

L

A

T

E

E

A

A

K

R

G

V

E

E

I

V

S

L

Y

S

A

A

S

D

F

I

I

I

E

E

W

W

F

F

A

A

E

D

E

E

G

G

K

R

R

R

I

V

Y

Y

G

G

D

R

T

I

I

V

P

P

G

P

H

R

Q

N

A

L

D

G

K

A

R

Q

L

Q

I

T

V

V

I

V

K

K

Q

E

P

P

I

V

G

G

V

P

T

V

A

A

I

F

T

T

P

P

W

W

N
|
N

F

F

P

P

A

V

A

N

M

Q

I

V

T

V

R

R

K

K

A

L

G

S

P

A

A

A

L

L

A

A

A

T

G

G

C

C

T

S

M

F

V

L

L

V

K
|
K

P

A

A

P

S

E

Q

E

T

T

P

P

F

A

S

S

A

P

L

A

A

A

L

L

A

L

E

R

L

A

A

F

I

V

R

D

A

A

G

G

V

V

P

P

A

A

G

G

V

V

F

I

N

G

V

L

V

V

T

Y

G

G

S

D

A

P

G

A

A

E

V

I

G

S

N

S

E

Y

L

L

T

I

S

P

N

H

P

P

L

V

V

I

R

R

K

K

L

V

S

T

F

F

T

T

G

G

S

S

T

T

E

P

I

V

G

G

R

K

Q

Q

L

L

M
x
A

E

S

Q

L

C

A

A

G

K

L

D

H

I

M

K

K

K

R

V

A

S

T

L

M

E
|
E

L

L

G

G

N

H

A

A

P

P

F

V

I

I

V

V

F

A

D

E

D

D

A

A

D

D

L

V

D

A

K

L

A

A

V

V

E

K

G

A

A

A

L

G

A

G

S

A

K

K

F

F

R

R

N

N

A

A

G

G

Q

Q

T

V

C
|
C

V

I

C

S

A

P

N

T

R

R

L

F

Y

L

V

V

Q

H

D

N

G

S

V

I

Y

R

D

D

R

E

F

F

A

T

E

R

K

A

L

L

Q

V

Q

K

A

H

V

A

S

E

K

G

L

L

H

K

I

V

G

G

D

N

G

G

L

L

D

E

N

E

G

G

V

T

T

T

I

L

G

G

P

A

L

L

I

A

D

N

E

P

K

R

A

R

V

L

A

T

K

A

V

M

E

A

E

S

H

V

I

I

A

D

D

N

A

A

L

R

E

K

K

V

G

G

A

A

R

S

V

I

V

E

C

T

G

G

K

E

A

R

H

I

E

G

R

S

G

E

G

G

N

N

F

F

Q

A

P

P

T

T

I

V

L

I

V

A

D

N

V

V

P

P

A

L

N

D

A

A

K

D

V

V

S

F

K

N

E

N

E
|
E

T

P

F

F

G

G

P

P

L

V

A

A

P

A

L

I

F

R

R

G

F

F

K

D

D

K

E

L

A

E

D

E

V

A

I

I

A

A

Q

E

A

A

N

N

D

R

T

L

E

P

F

F

G

G

L

L

A

A

A

G

Y

Y

F

A

Y

F

A

T

R

R

D

S

L

F

S

A

R

N

V

V

F

H

R

L

V

L

G

T

E

Q

A

R

L

L

E

E

Y

V

G

G

I

M

V

L

G

W

I

I

N

N

T

Q

G

P

I

A

I

T

S

P

N

W

E

P

V

E

A

M

P

P

F

F

G

G

I

V

K

K

A

D

S

S

G

G

L
x
Y

G

G

R

S

E
|
E

G

G

S

G

K

P

Y

E

G

A

I

L

E

E

D

P

Y

Y

L

L

E

V

I

T

K

K

Y

S

M

V

C

T

I

V

active site: N151 (= N157), K174 (= K180), E249 (= E255), C283 (= C289), E380 (= E386), E457 (= E463)
binding glycerol: A236 (≠ M242), Y454 (≠ L460)

6j76A Structure of 3,6-anhydro-l-galactose dehydrogenase in complex with nap (see paper)
39% identity, 98% coverage: 10:480/482 of query aligns to 1:475/477 of 6j76A

query
sites
6j76A

R

Q

Q

Y

Q

Q

A

M

L

Y

I

V

N

G

G

G

E

E

W

W

L

I

D

D

A

A

N

S

N

N

G

G

E

Q

A

T

I

E

D

P

V

V

T

V

N

S

P

P

A

I

N

N

G

E

D

E

K

V

L

L

G

A

S

Y

V

I

P

Q

K

D

M

A

G

D

A

A

D

S

E

D

T

A

R

E

A

R

A

V

I

L

D

A

A

V

A

A

N

T

R

T

A

A

L

Q

P

K

A

E

W

W

R

A

A

K

L

Q

T

P

A

A

K

R

E

Q

R

R

A

A

T

E

I

V

L

L

R

R

N

K

W

F

F

A

N

Q

L

L

M

I

M

R

E

D

H

N

Q

K

D

Q

D

Y

L

L

A

A

R

E

L

L

M

L

T

V

L

K

E

E

Q

Q

G

G

K

K

P

L

L

L

A

K

E

V

A

A

K

L

G

G

E

E

I

V

S

E

Y

A

A

T

A

S

S

T

F

F

I

I

E

E

W

Y

F

A

A

C

E

D

E

W

G

A

K

R

R

Q

I

M

Y

D

G

G

D

D

T

I

I

V

P

K

G

S

H

D

Q

N

A

A

D

N

K

E

R

Q

L

I

I

M

V

I

I

H

K

K

Q

I

P

P

I

R

G

G

V

V

T

V

A

V

A

A

I
|
I

T
|
T

P
x
A

W
|
W

N
|
N

F

F

P

P

A

L

A

A

M

L

I

A

T

G

R

R

K

K

A

I

G

G

P

P

A

A

L

L

A

V

A

A

G

G

C

N

T

S

M

I

V

V

L

V

K
|
K

P

P

A
x
T

S
|
S

Q

E

T

T

P

P

F

L

S

A

A

T

L

L

A

E

L

L

A

G

E

Y

L

L

A

A

I

E

R

Q

A

A

G

G

V

I

P

P

A

A

G

G

V

V

F

L

N

N

V

I

V

V