SitesBLAST
Comparing 16981 FitnessBrowser__Keio:16981 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4f66A The crystal structure of 6-phospho-beta-glucosidase from streptococcus mutans ua159 in complex with beta-d-glucose-6-phosphate. (see paper)
64% identity, 99% coverage: 8:479/479 of query aligns to 5:478/478 of 4f66A
- active site: R86 (= R89), H131 (= H134), E177 (= E180), N180 (= N183), S312 (= S315), Y314 (= Y317), E376 (= E377)
- binding 6-O-phosphono-beta-D-glucopyranose: Q19 (= Q22), H131 (= H134), F132 (= F135), E177 (= E180), Y314 (= Y317), W350 (= W351), E376 (= E377), W424 (= W425), S431 (= S432), A432 (≠ F433), G433 (≠ T434), K439 (= K440), Y441 (= Y442)
4f79A The crystal structure of 6-phospho-beta-glucosidase mutant (e375q) in complex with salicin 6-phosphate (see paper)
64% identity, 99% coverage: 8:479/479 of query aligns to 5:478/478 of 4f79A
- active site: R86 (= R89), H131 (= H134), E177 (= E180), N180 (= N183), S312 (= S315), Y314 (= Y317), Q376 (≠ E377)
- binding 2-(hydroxymethyl)phenyl 6-O-phosphono-beta-D-glucopyranoside: Q19 (= Q22), H131 (= H134), F132 (= F135), E177 (= E180), Y314 (= Y317), W350 (= W351), Q376 (≠ E377), W424 (= W425), S431 (= S432), A432 (≠ F433), G433 (≠ T434), K439 (= K440), Y441 (= Y442)
4gpnB The crystal structure of 6-p-beta-d-glucosidase (e375q mutant) from streptococcus mutans ua150 in complex with gentiobiose 6-phosphate. (see paper)
64% identity, 99% coverage: 8:479/479 of query aligns to 3:476/476 of 4gpnB
- active site: R84 (= R89), H129 (= H134), E175 (= E180), N178 (= N183), S310 (= S315), Y312 (= Y317), Q374 (≠ E377)
- binding 6-O-phosphono-beta-D-glucopyranose: Q17 (= Q22), H129 (= H134), F130 (= F135), E175 (= E180), Y312 (= Y317), W348 (= W351), Q374 (≠ E377), W422 (= W425), S429 (= S432), A430 (≠ F433), G431 (≠ T434), K437 (= K440), Y439 (= Y442)
- binding beta-D-glucopyranose: E175 (= E180), W348 (= W351)
3qomA Crystal structure of 6-phospho-beta-glucosidase from lactobacillus plantarum (see paper)
58% identity, 100% coverage: 1:479/479 of query aligns to 2:478/479 of 3qomA
- active site: R90 (= R89), H135 (= H134), E181 (= E180), N184 (= N183), S316 (= S315), Y318 (= Y317), E376 (= E377)
- binding beta-D-glucopyranose: E181 (= E180), N184 (= N183), A244 (= A243), R268 (= R267), W350 (= W351)
- binding phosphate ion: Y28 (≠ W27), H65 (= H64), H202 (≠ Y201), P204 (≠ E203), G205 (≠ H204), W350 (= W351), A431 (≠ S432), A432 (≠ F433), S433 (≠ T434), K439 (= K440), Y441 (= Y442)
4ipnE The complex structure of 6-phospho-beta-glucosidase bgla-2 with thiocellobiose-6p from streptococcus pneumoniae (see paper)
49% identity, 99% coverage: 8:479/479 of query aligns to 3:462/463 of 4ipnE
4ipnB The complex structure of 6-phospho-beta-glucosidase bgla-2 with thiocellobiose-6p from streptococcus pneumoniae (see paper)
50% identity, 99% coverage: 8:479/479 of query aligns to 3:456/457 of 4ipnB
- active site: R79 (= R89), H124 (= H134), E170 (= E180), S173 (≠ N183), S300 (= S315), Y302 (= Y317), E351 (= E377)
- binding 6-O-phosphono-alpha-L-idopyranose: Q17 (= Q22), H124 (= H134), N169 (= N179), E351 (= E377), W402 (= W425), S409 (= S432), M410 (≠ F433), S411 (≠ T434)
- binding 4-thio-beta-D-glucopyranose: Y125 (≠ F135), E170 (= E180), S173 (≠ N183)
4zfmC Structure of gan1d-e170q in complex with cellobiose-6-phosphate
40% identity, 99% coverage: 8:479/479 of query aligns to 5:461/461 of 4zfmC
- active site: R76 (= R89), H120 (= H134), Q166 (≠ E180), I169 (≠ N183), N295 (≠ S315), Y297 (= Y317), E361 (= E377)
- binding 1,5-anhydro-6-O-phosphono-D-glucitol: Q19 (= Q22), H120 (= H134), Q166 (≠ E180), E361 (= E377), W408 (= W425), S415 (= S432), W416 (≠ F433), Y424 (= Y442)
- binding beta-D-glucopyranose: Q166 (≠ E180), Y297 (= Y317)
5okbB High resolution structure of native gan1d, a putative 6-phospho-beta- galactosidase from geobacillus stearothermophilus (see paper)
39% identity, 99% coverage: 8:479/479 of query aligns to 5:474/474 of 5okbB
4zepA Structure of gan1d, a 6-phospho-beta-galactosidase from geobacillus stearothermophilus, in complex with 6-phospho-glucose
39% identity, 99% coverage: 8:479/479 of query aligns to 5:474/474 of 4zepA
- active site: R76 (= R89), H120 (= H134), E166 (= E180), I169 (≠ N183), N295 (≠ S315), Y297 (= Y317), E374 (= E377)
- binding 6-O-phosphono-beta-D-glucopyranose: Q19 (= Q22), E166 (= E180), Y297 (= Y317), W348 (= W351), E374 (= E377), W421 (= W425), S428 (= S432), K435 (= K440), Y437 (= Y442)
8b80A The structure of gan1d w433a in complex with galactose-6p
39% identity, 99% coverage: 8:479/479 of query aligns to 5:474/474 of 8b80A
6z1mA Structure of an ancestral glycosidase (family 1) bound to heme (see paper)
36% identity, 98% coverage: 6:476/479 of query aligns to 1:422/423 of 6z1mA
- binding protoporphyrin ix containing fe: P164 (≠ I181), N165 (= N182), L194 (≠ Q218), L195 (≠ F219), L218 (= L242), L220 (≠ M244), N244 (≠ R267), F247 (= F270), K253 (≠ R276), Y256 (= Y279), L288 (≠ C309), R318 (≠ E364), Y323 (= Y369)
- binding magnesium ion: H346 (≠ N393), K409 (= K463)
5yifA Pyruvylated beta-d-galactosidase from bacillus sp. Hma207, e163a mutant pyruvylated beta-d-galactose complex (see paper)
34% identity, 99% coverage: 6:479/479 of query aligns to 1:459/459 of 5yifA
- binding (2R,4aR,6R,7R,8R,8aR)-2-methyl-6,7,8-tris(oxidanyl)-4,4a,6,7,8,8a-hexahydropyrano[3,2-d][1,3]dioxine-2-carboxylic acid: Q17 (= Q22), H118 (= H134), N162 (= N179), N295 (≠ S315), Y297 (= Y317), W337 (= W351), E364 (= E377), W410 (= W425), S417 (= S432), W418 (≠ F433), K424 (= K440)
4ptwA Halothermothrix orenii beta-glucosidase a, 2-deoxy-2-fluoro-glucose complex (see paper)
34% identity, 97% coverage: 6:472/479 of query aligns to 1:440/445 of 4ptwA
- active site: R74 (= R89), H118 (= H134), E163 (= E180), V166 (≠ R188), N291 (≠ S315), Y293 (= Y317), E351 (= E377)
- binding 2-deoxy-2-fluoro-alpha-D-glucopyranose: Q17 (= Q22), H118 (= H134), E163 (= E180), Y293 (= Y317), E351 (= E377), W398 (= W425), E405 (≠ S432), W406 (≠ F433)
4ptvA Halothermothrix orenii beta-glucosidase a, thiocellobiose complex (see paper)
34% identity, 97% coverage: 6:472/479 of query aligns to 1:440/445 of 4ptvA
- active site: R74 (= R89), H118 (= H134), E163 (= E180), V166 (≠ R188), N291 (≠ S315), Y293 (= Y317), E351 (= E377)
- binding beta-D-glucopyranose: W324 (= W351), E405 (≠ S432), Y408 (≠ T435)
- binding 4-thio-beta-D-glucopyranose: Q17 (= Q22), H118 (= H134), E163 (= E180), Y293 (= Y317), E351 (= E377), W398 (= W425), E405 (≠ S432), W406 (≠ F433)
8ivyA Beta-glucosidase bgla mutant e166q in complex with glucose (see paper)
33% identity, 99% coverage: 3:476/479 of query aligns to 1:447/451 of 8ivyA
Q9FIW4 Beta-glucosidase 42; AtBGLU42; EC 3.2.1.21 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
31% identity, 99% coverage: 2:477/479 of query aligns to 15:484/490 of Q9FIW4
- R342 (≠ P342) Important for substrate chain length specificity; mutation R->A,Y: Highest preference on cellotetraose and cellopentaose with increased affinities at subsite (+)3.
6r4kA Structure of beta-glucosidase a from paenibacillus polymyxa complexed with a monovalent inhibitor (see paper)
31% identity, 98% coverage: 8:475/479 of query aligns to 6:442/448 of 6r4kA
- binding (2~{S},3~{S},4~{R})-2-[[4-[4-[2-[2-(2-azanylidenehydrazinyl)ethoxy]ethoxy]phenyl]-1,2,3-triazol-1-yl]methyl]pyrrolidine-3,4-diol: Q20 (= Q22), H121 (= H134), E166 (= E180), L173 (≠ F192), E352 (= E377), W398 (= W425), E405 (≠ S432), W406 (≠ F433)
6qwiA Structure of beta-glucosidase a from paenibacillus polymyxa complexed with multivalent inhibitors. (see paper)
31% identity, 98% coverage: 8:475/479 of query aligns to 6:442/448 of 6qwiA
- binding (2~{S},3~{S},4~{R})-2-[[4-[4-(2-ethoxyethoxy)phenyl]-1,2,3-triazol-1-yl]methyl]pyrrolidine-3,4-diol: Q20 (= Q22), H121 (= H134), W122 (≠ F135), E166 (= E180), L173 (≠ F192), W326 (= W351), E352 (= E377), W398 (= W425), E405 (≠ S432), W406 (≠ F433)
1bggB Glucosidase a from bacillus polymyxa complexed with gluconate (see paper)
31% identity, 98% coverage: 8:475/479 of query aligns to 5:441/447 of 1bggB
- active site: R76 (= R89), H120 (= H134), E165 (= E180), C168 (≠ R188), N293 (≠ S315), Y295 (= Y317), E351 (= E377)
- binding D-gluconic acid: Q19 (= Q22), H120 (= H134), W121 (≠ F135), N164 (= N179), E165 (= E180), Y295 (= Y317), E351 (= E377), W397 (= W425), E404 (≠ S432), W405 (≠ F433)
1e4iA 2-deoxy-2-fluoro-beta-d-glucosyl/enzyme intermediate complex of the beta-glucosidase from bacillus polymyxa (see paper)
31% identity, 98% coverage: 8:475/479 of query aligns to 5:441/447 of 1e4iA
- active site: R76 (= R89), H120 (= H134), E165 (= E180), C168 (≠ R188), N293 (≠ S315), Y295 (= Y317), E351 (= E377)
- binding 2-deoxy-2-fluoro-alpha-D-glucopyranose: Q19 (= Q22), H120 (= H134), N164 (= N179), E165 (= E180), Y295 (= Y317), E351 (= E377), W397 (= W425), E404 (≠ S432), W405 (≠ F433)
- binding 2,4-dinitrophenyl 2-deoxy-2-fluoro-beta-D-glucopyranoside: R136 (= R151), Q140 (≠ D155), V143 (= V158), L202 (= L224)
Query Sequence
>16981 FitnessBrowser__Keio:16981
MIVKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHGVPREITKEVLPGKY
YPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEAQPNEEGLKFYDDMFDE
LLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAEVVFERYKHKVKYWMTFNE
INNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFVASALAVKAARRINPEMKVGC
MLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGYYPSYVLNEWERRGFNIKMEDGD
LDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFEGSVPNPYVKASDWGWQIDPVGLRY
ALCELYERYQRPLFIVENGFGAYDKVEEDGSINDDYRIDYLRAHIEEMKKAVTYDGVDLM
GYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDDGTGDMSRSRKKSFNWYKEVIASNGEKL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory