SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 17043 FitnessBrowser__Keio:17043 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

P0AFF4 Nucleoside permease NupG; Nucleoside-transport system protein NupG from Escherichia coli (strain K12) (see paper)
100% identity, 100% coverage: 1:418/418 of query aligns to 1:418/418 of P0AFF4

query
sites
P0AFF4

M

M

N

N

L

L

K

K

L

L

Q

Q

L

L

K

K

I

I

L

L

S

S

F

F

L

L

Q

Q

F

F

C

C

L

L

W

W

G

G

S

S

W

W

L

L

T

T

T

T

L

L

G

G

S

S

Y

Y

M

M

F

F

V

V

T

T

L

L

K

K

F

F

D

D

G

G

A

A

S

S

I

I

G

G

A

A

V

V

Y

Y

S

S

S

S

L

L

G

G

I

I

A

A

A

A

V

V

F

F

M

M

P

P

A

A

L

L

L

L

G

G

I

I

V

V

A

A

D

D

K

K

W

W

L

L

S

S

A

A

K

K

W

W

V

V

Y

Y

A

A

I

I

C

C

H

H

T

T

I

I

G

G

A

A

I

I

T

T

L

L

F

F

M

M

A

A

A

A

Q

Q

V

V

T

T

T

T

P

P

E

E

A

A

M

M

F

F

L

L

V

V

I

I

L

L

I

I

N

N

S

S

F

F

A

A

Y

Y

M

M

P

P

T

T

L

L

G

G

L

L

I

I

N

N

T

T

I

I

S

S

Y

Y

Y

Y

R

R

L

L

Q

Q

N

N

A

A

G

G

M

M

D

D

I

I

V

V

T

T

D

D

F

F

P

P

P

P

I

I

R
|
R

I

I

W

W

G

G

T
|
T

I

I

G

G

F
|
F

I

I

M

M

A

A

M

M

W

W

V

V

V

V

S

S

L

L

S

S

G

G

F

F

E

E

L

L

S

S

H

H

M

M

Q

Q

L

L

Y

Y

I

I

G

G

A

A

A

A

L

L

S

S

A

A

I

I

L

L

V

V

L

L

F

F

T

T

L

L

T

T

L

L

P

P

H

H

I

I

P

P

V

V

A

A

K

K

Q

Q

Q

Q

A

A

N

N

Q

Q

S

S

W

W

T

T

T

T

L

L

L

L

G

G

L

L

D

D

A

A

F

F

A

A

L

L

F

F

K

K

N

N

K

K

R

R

M

M

A

A

I

I

F

F

F

F

I

I

F

F

S

S

M

M

L

L

L

L

G

G

A

A

E

E

L

L

Q
|
Q

I

I

T

T

N
|
N

M

M

F

F

G

G

N

N

T

T

F

F

L

L

H

H

S

S

F

F

D

D

K

K

D

D

P

P

M

M

F

F

A

A

S

S

S

S

F

F

I

I

V

V

Q

Q

H

H

A

A

S

S

I

I

I

I

M

M

S

S

I

I

S

S

Q
|
Q

I

I

S

S

E
|
E

T

T

L

L

F

F

I

I

L

L

T

T

I

I

P

P

F

F

F

F

L

L

S

S

R

R

Y

Y

G

G

I

I

K

K

N

N

V

V

M

M

M

M

I

I

S

S

I

I

V

V

A

A

W

W

I

I

L

L

R

R

F

F

A

A

L

L

F

F

A

A

Y

Y

G

G

D

D

P

P

T

T

P

P

F

F

G

G

T

T

V

V

L

L

L

L

V

V

L

L

S

S

M

M

I

I

V

V

Y
|
Y

G

G

C

C

A

A

F
|
F

D
|
D

F

F

F

F

N

N

I

I

S

S

G

G

S

S

V

V

F

F

V

V

E

E

K

K

E

E

V

V

S

S

P

P

A

A

I

I

R

R

A

A

S

S

A

A

Q

Q

G

G

M

M

F

F

L

L

M

M

M

M

T

T

N

N

G

G

F

F

G

G

C

C

I

I

L

L

G

G

G

G

I

I

V

V

S

S

G

G

K

K

V

V

V

V

E

E

M

M

Y

Y

T

T

Q

Q

N

N

G

G

I

I

T

T

D

D

W

W

Q

Q

T

T

V

V

W

W

L

L

I

I

F

F

A

A

G

G

Y

Y

S

S

V

V

V

V

L

L

A

A

F

F

A

A

F

F

M

M

A

A

M

M

F

F

K

K

Y

Y

K

K

H

H

V

V

R

R

V

V

P

P

T

T

G

G

T

T

Q

Q

T

T

V

V

S

S

H

H

R136 (= R136) mutation to A: Abolishes the binding affinity for uridine.
T140 (= T140) mutation to A: Abolishes the binding affinity for uridine.
F143 (= F143) mutation to A: Abolishes the binding affinity for uridine.
Q225 (= Q225) mutation to A: No change in the binding affinity for uridine.
N228 (= N228) mutation to A: Abolishes the binding affinity for uridine.
Q261 (= Q261) mutation to A: Abolishes the binding affinity for uridine.
E264 (= E264) mutation to A: Abolishes the binding affinity for uridine.
Y318 (= Y318) mutation to A: Abolishes the binding affinity for uridine.
F322 (= F322) mutation to A: Abolishes the binding affinity for uridine.
D323 (= D323) mutation to A: 20-fold increase in the binding affinity for uridine.

Query Sequence

>17043 FitnessBrowser__Keio:17043
MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVYSSLGIAAVFMPALLGI
VADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILINSFAYMPTLGLINTISYYR
LQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHMQLYIGAALSAILVLFTLTLP
HIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSMLLGAELQITNMFGNTFLHSFDK
DPMFASSFIVQHASIIMSISQISETLFILTIPFFLSRYGIKNVMMISIVAWILRFALFAY
GDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVEKEVSPAIRASAQGMFLMMTNGFGCIL
GGIVSGKVVEMYTQNGITDWQTVWLIFAGYSVVLAFAFMAMFKYKHVRVPTGTQTVSH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory