SitesBLAST

3ws7A The 1.18 a resolution structure of l-serine 3-dehydrogenase complexed with NADP+ and sulfate ion from the hyperthermophilic archaeon pyrobaculum calidifontis (see paper)
39% identity, 96% coverage: 1:283/294 of query aligns to 14:293/293 of 3ws7A

query
sites
3ws7A

M

M

K

R

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V

G

G

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F

I

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L
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L

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I
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M

G

G

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G

P

P

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M

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A

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T

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H

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L

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K

A

A

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L

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x
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R

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x
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K

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K

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P

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I

A

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E

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G

G

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Y

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D

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M

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G

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Y

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P

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Y

binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G7), L21 (= L8), G22 (= G9), I23 (= I10), M24 (= M11), N43 (≠ D30), R44 (= R31), T45 (≠ N32), K48 (≠ A35), M76 (= M63), V77 (≠ L64), S78 (≠ P65), D82 (≠ H69), Q85 (≠ E72), V133 (= V120), F241 (= F231), K242 (≠ R232), H245 (≠ L235), K248 (= K238)
binding sulfate ion: T134 (≠ S121), G135 (= G122), K183 (= K170)

3pefA Crystal structure of gamma-hydroxybutyrate dehydrogenase from geobacter metallireducens in complex with NADP+ (see paper)
37% identity, 97% coverage: 2:286/294 of query aligns to 3:287/287 of 3pefA

query
sites
3pefA

K

K

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F

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G

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F

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G

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A

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A

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K

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N

L

L

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K

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x
A

A

L

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K

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M

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D

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R

N

N

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R

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T

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K

R

binding glycerol: D67 (≠ N66), G123 (= G122), K171 (= K170), N175 (≠ Q174), M178 (≠ V177), L203 (≠ Y202), G207 (≠ R206), N213 (≠ S212), A217 (≠ D216), F232 (= F231), H236 (≠ L235), K239 (= K238), R242 (≠ A241), R269 (= R268)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G9), I11 (= I10), M12 (= M11), N31 (≠ D30), R32 (= R31), S33 (≠ N32), K36 (≠ A35), M64 (= M63), L65 (= L64), A66 (≠ P65), A70 (≠ H69), E73 (= E72), T96 (≠ S95), V121 (= V120), G123 (= G122), S124 (≠ G123), A231 (≠ G230), F232 (= F231), H236 (≠ L235), K239 (= K238)

5je8B The crystal structure of bacillus cereus 3-hydroxyisobutyrate dehydrogenase in complex with NAD (see paper)
35% identity, 98% coverage: 2:289/294 of query aligns to 5:291/294 of 5je8B

query
sites
5je8B

K

K

V

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G

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F

F

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G

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N

M
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M

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K

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N

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D

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L

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V

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D

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A

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K

N

-

Y

A

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M

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M

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E

N

N

F

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K

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N

L

L

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K

K

D

D

L

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N

F

A

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A

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K

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H

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P

L

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S

A

E

A

M

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M

L

E

N

M

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M

Y

Q

D

A

E

L

A

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S

A

Q

D

A

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G

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Y

G

G

T

E

A

N

D

D

H

M

S

A

A

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L

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C

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Y

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Y

E

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K

K

L

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K

E

binding nicotinamide-adenine-dinucleotide: G12 (= G9), N13 (≠ I10), M14 (= M11), D33 (= D30), L34 (≠ R31), L67 (= L64), P68 (= P65), V76 (= V73), S97 (= S94), V123 (= V120)

P29266 3-hydroxyisobutyrate dehydrogenase, mitochondrial; HIBADH; EC 1.1.1.31 from Rattus norvegicus (Rat) (see paper)
35% identity, 96% coverage: 3:283/294 of query aligns to 41:328/335 of P29266

query
sites
P29266

V

V

G

G

F

F

I

I

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G

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L

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I

N

M

M

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A

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K

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N

L

L

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K

K

A

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D
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D

R

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A

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D

E

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A

E

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A

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A

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M

M

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H

N

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K

I

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A

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G

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A

N

N

G

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I

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M

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M

M

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G

A

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G

G

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K
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K

L

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A

C

N

N

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x
N

V

M

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V

L

A

A

L

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A

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M

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S

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A

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K

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A

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P

M

G

V

V

M

M

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D

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G

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N

N

N

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P

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G

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F

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G

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L

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K

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Q

Y

Y

D68 (= D30) mutation to R: Decrease of activity with NAD, increase of activity with NADP.
K208 (= K170) mutation K->A,H,N,R: Complete loss of activity.
N212 (≠ Q174) mutation to Q: Decrease in activity.

2i9pB Crystal structure of human hydroxyisobutyrate dehydrogenase complexed with NAD+
36% identity, 95% coverage: 1:280/294 of query aligns to 1:287/296 of 2i9pB

query
sites
2i9pB

M

M

K

P

V

V

G

G

F

F

I

I

G

G

L

L

G
|
G

I
x
N

M
|
M

G

G

K

N

P

P

M

M

S

A

K

K

N

N

L

L

L

M

K

K

A

H

G

G

Y

Y

S

P

L

L

V

I

A
x
Y

D
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D

R
x
V

N

F

P

P

E

D

A

A

I

C

A

K

D

E

V

F

I

Q

A

D

A

A

G

G

A

E

E

Q

T

V

A

V

S

S

T

S

A

P

K

A

A

D

I

V

A

A

E

E

Q

K

C

A

D

D

V

R

I

I

T

T

M
|
M

L
|
L

P
|
P

N

T

S

S

P

I

H

N

V

A

K

I

E

E

V

A

A

Y

L

S

G

G

E

A

N

N

G

G

I

I

I

L

E

K

G

K

A

V

K

K

P

K

G

G

T

S

V

L

L

L

I

I

D

D

M

S

S

S

S
x
T

I

I

A

D

P

P

L

A

A

V

S

S

R

K

E

E

I

L

S

A

E

K

A

E

L

V

K

E

A

K

K

M

G

G

I

A

D

V

M

F

L

M

D

D

A

A

P

P

V
|
V

S

S

G
|
G

G

G

E

V

P

G

K

A

A

A

I

R

D

S

G

G

T

N

L

L

S

T

V

F

M

M

V

V

G

G

D

V

K

E

A

D

I

E

F

F

D

A

K

A

Y

A

Y

Q

D

E

L

L

M

L

K

G

A

C

M

M

A

G

G

S

S

N

V

V

H

Y

T

C

G

G

E

A

I

V

G

G

A

T

G

G

N

Q

V

A

T

A

K

K

L

I

A

C

N

N

Q

N

V

M

I

L

V

L

A

A

L

I

N

S

I

M

A

I

A

G

M

T

S

A

E

E

A

A

L

M

T

N

L

L

A

G

T

I

K

R

A

L

G

G

V

L

N

D

P

P

D

K

L

L

V

L

Y

A

Q

K

A

I

I

L

R

N

-

M

-

S

G

S

G

G

L

R

A

C

G

W

S

S

T

S

V

D

L

T

D

Y

A

N

K

P

A

V

P

P

M

G

V

V

M

M

D

D

-

G

-

V

-

P

-

S

-

A

R

N

N

N

F

Y

K

Q

P

G

G

G

F
|
F

R

G

I

T

D

T

L

L

H

M

I

A

K
|
K

D

D

L

L

A

G

N

L

A

A

L

Q

D

D

T

S

S

A

H

T

G

S

V

T

G

K

A

S

Q

P

L

I

P

L

L

L

T

G

A

S

A

L

V

A

M

H

E

Q

M

I

M

Y

Q

R

A

M

L

M

R

C

A

A

D

K

G

G

L

Y

G

S

T

K

A

K

D

D

H

F

S

S

A

S

L

V

binding nicotinamide-adenine-dinucleotide: G9 (= G9), N10 (≠ I10), M11 (= M11), Y29 (≠ A29), D30 (= D30), V31 (≠ R31), M63 (= M63), L64 (= L64), P65 (= P65), T95 (≠ S95), V120 (= V120), G122 (= G122), F238 (= F231), K245 (= K238)

P31937 3-hydroxyisobutyrate dehydrogenase, mitochondrial; HIBADH; EC 1.1.1.31 from Homo sapiens (Human) (see paper)
35% identity, 95% coverage: 3:280/294 of query aligns to 42:326/336 of P31937

query
sites
P31937

V
|
V

G
|
G

F
|
F

I
|
I

G
|
G

L
|
L

G
|
G

I
x
N

M
|
M

G
|
G

K
x
N

P
|
P

M
|
M

S
x
A

K
|
K

N
|
N

L
|
L

L
x
M

K
|
K

A
x
H

G
|
G

Y
|
Y

S
x
P

L
|
L

V
x
I

A
x
Y

D

D

R

V

N

F

P

P

E

D

A

A

I

C

A

K

D

E

V

F

I

Q

A

D

A

A

G

G

A

E

E

Q

T

V

A

V

S

S

T

S

A

P

K

A

A

D

I

V

A

A

E

E

Q

K

C

A

D

D

V

R

I

I

T

T

M

M

L
|
L

P
|
P

N

T

S

S

P

I

H
x
N

V

A

K

I

E

E

V

A

A

Y

L

S

G

G

E

A

N

N

G

G

I

I

I

L

E

K

G

K

A

V

K

K

P

K

G

G

T

S

V

L

L

L

I

I

D

D

M

S

S

S

S
x
T

I

I

A

D

P

P

L

A

A

V

S

S

R

K

E

E

I

L

S

A

E

K

A

E

L

V

K

E

A

K

K

M

G

G

I

A

D

V

M

F

L

M

D

D

A

A

P

P

V

V

S

S

G

G

E

V

P

G

K

A

A

A

I

R

D

S

G

G

T

N

L

L

S

T

V

F

M

M

V

V

G

G

D

V

K

E

A

D

I

E

F

F

D

A

K

A

Y

A

Y

Q

D

E

L

L

M

L

K

G

A

C

M

M

A

G

G

S

S

N

V

V

H

Y

T

C

G

G

E

A

I

V

G

G

A

T

G

G

N

Q

V

A

T

A

K

K

L

I

A

C

N

N

Q

N

V

M

I

L

V

L

A

A

L

I

N

S

I

M

A

I

A

G

M

T

S

A

E

E

A

A

L

M

T

N

L

L

A

G

T

I

K

R

A

L

G

G

V

L

N

D

P

P

D

K

L

L

V

L

Y

A

Q

K

A

I

I

L

R

N

-

M

-

S

G

S

G

G

L

R

A

C

G

W

S

S

T

S

V

D

L

T

D

Y

A

N

K

P

A

V

P

P

M

G

V

V

M

M

D

D

-

G

-

V

-

P

-

S

-

A

R

N

N

N

F

Y

K

Q

P

G

G

G

F

F

R

G

I

T

D

T

L

L

H

M

I

A

K
|
K

D

D

L

L

A

G

N

L

A

A

L

Q

D

D

T

S

S

A

H

T

G

S

V

T

G

K

A

S

Q

P

L

I

P

L

L

L

T

G

A

S

A

L

V

A

M

H

E

Q

M

I

M

Y

Q

R

A

M

L

M

R

C

A

A

D

K

G

G

L

Y

G

S

T

K

A

K

D

D

H

F

S

S

A

S

L

V

LP 103:104 (= LP 64:65) binding NAD(+)
N108 (≠ H69) binding NAD(+)
T134 (≠ S95) binding NAD(+)
K284 (= K238) binding NAD(+)

Sites not aligning to the query:

1:36 modified: transit peptide, Mitochondrion
40:68 binding NAD(+)

6smzC Crystal structure of sla reductase yihu from e. Coli in complex with nadh
37% identity, 95% coverage: 3:280/294 of query aligns to 3:281/295 of 6smzC

query
sites
6smzC

V

I

G

A

F

F

I

I

G

G

L

L

G
|
G

I
x
Q

M
|
M

G

G

K

S

P

P

M

M

S

A

K

S

N

N

L

L

K

Q

A

Q

G

G

Y

H

S

Q

L

L

V

R

V

V

A
x
F

D
|
D

R
x
V

N

N

P

A

E

E

A

A

I

V

A

R

D

H

V

L

I

V

A

D

A

K

G

G

A

A

E

T

T

P

A

A

S

A

T

N

A

P

K

A

A

Q

I

A

A

A

E

K

Q

D

C

A

D

E

V

F

I

I

T

T

M
|
M

L
|
L

P

P

N

N

S

G

P

D

H

L

V

V

K

R

E

N

V
|
V

A

L

L

F

G

G

E

E

N

N

G

G

I

V

I

C

E

E

G

G

A

L

K

S

P

T

G

D

T

A

V

L

L

V

I

I

D

D

M

M

S
|
S

S
x
T

I

I

A

H

P

P

L

L

A

Q

S

T

R

D

E

K

I

L

S

I

E

A

A

D

L

M

K

Q

A

A

K

K

G

G

I

F

D

S

M

M

L

M

D

D

A

V

P

P

V

V

S

G

G
x
R

G

T

E

S

P

A

K

N

A

A

I

I

D

T

G

G

T

T

L

L

S

L

V

L

M

L

V

A

G

G

D

T

K

A

A

E

I

Q

F

V

D

E

K

R

Y

A

Y

T

D

P

L

I

M

L

K

M

A

A

M

M

A

G

G

S

S

E

V

L

V

I

H

N

T

A

G

G

E

G

I

P

G

G

A

M

G

G

N

I

V

R

T

V

K

K

L

L

A

I

N

N

Q

N

V

Y

I

M

-

S

V

I

A

A

L

L

N

N

I

-

A

A

A

L

M

S

S

A

E

E

A

A

L

A

T

V

L

L

A

C

T

E

K

A

A

L

G

N

V

L

N

P

P

F

D

D

L

V

V

A

Y

V

Q

K

A

V

I

M

R

S

G

G

G

T

L

A

A

A

G

G

S

K

T

G

V

H

L

F

D

T

A

T

K

S

A

W

P

P

-

N

M

K

V

V

M

L

D

S

R

G

N

D

F

L

K

S

P

P

G

A

F

F

R

M

I

I

D

D

L

L

H

A

I

H

K

K

D

D

L

L

A

G

N

I

A

A

L

L

D

D

T

V

S

A

H

N

G

Q

V

L

G

H

A

V

Q

P

L

M

P

P

L

L

T

G

A

A

V

S

M

R

E

E

M

V

M

Y

Q

S

A

Q

L

A

R

R

A

A

D

A

G

G

L

R

G

G

T

R

A

Q

D

D

H

W

S

S

A

A

L

I

binding nicotinamide-adenine-dinucleotide: G9 (= G9), Q10 (≠ I10), M11 (= M11), F29 (≠ A29), D30 (= D30), V31 (≠ R31), M63 (= M63), L64 (= L64), V73 (= V73), S94 (= S94), T95 (≠ S95), R122 (≠ G122)

P0A9V8 3-sulfolactaldehyde reductase; SLA reductase; 4-hydroxybutyrate dehydrogenase; Gamma-hydroxybutyrate dehydrogenase; GHBDH; Succinic semialdehyde reductase; SSA reductase; EC 1.1.1.373; EC 1.1.1.61 from Escherichia coli (strain K12)
37% identity, 95% coverage: 3:280/294 of query aligns to 4:282/298 of P0A9V8

query
sites
P0A9V8

V

I

G

A

F

F

I

I

G

G

L

L

G

G

I
x
Q

M
|
M

G

G

K

S

P

P

M

M

S

A

K

S

N

N

L

L

K

Q

A

Q

G

G

Y

H

S

Q

L

L

V

R

V

V

A

F

D
|
D

R

V

N

N

P

A

E

E

A

A

I

V

A

R

D

H

V

L

I

V

A

D

A

K

G

G

A

A

E

T

T

P

A

A

S

A

T

N

A

P

K

A

A

Q

I

A

A

A

E

K

Q

D

C

A

D

E

V

F

I

I

T

T

M

M

L
|
L

P

P

N

N

S

G

P

D

H

L

V

V

K

R

E

N

V

V

A

L

L

F

G

G

E

E

N

N

G

G

I

V

I

C

E

E

G

G

A

L

K

S

P

T

G

D

T

A

V

L

L

V

I

I

D

D

M

M

S

S

S
x
T

I

I

A

H

P

P

L

L

A

Q

S

T

R

D

E

K

I

L

S

I

E

A

A

D

L

M

K

Q

A

A

K

K

G

G

I

F

D

S

M

M

L

M

D

D

A

V

P

P

V

V

S
x
G

G
x
R

G
x
T

E

S

P

A

K

N

A

A

I

I

D

T

G

G

T

T

L

L

S

L

V

L

M

L

V

A

G

G

D

T

K

A

A

E

I

Q

F

V

D

E

K

R

Y

A

Y

T

D

P

L

I

M

L

K

M

A

A

M

M

A

G

G

S

S

E

V

L

V

I

H

N

T

A

G

G

E

G

I

P

G

G

A

M

G

G

N

I

V

R

T

V

K

K

L

L

A

I

N
|
N

Q
x
N

V
x
Y

I
x
M

-
x
S

V

I

A

A

L

L

N

N

I

-

A

A

A

L

M

S

S

A

E

E

A

A

L

A

T

V

L

L

A

C

T

E

K

A

A

L

G

N

V

L

N

P

P

F

D

D

L

V

V

A

Y

V

Q

K

A

V

I

M

R

S

G

G

G

T

L

A

A

A

G

G

S

K

T

G

V

H

L

F

D

T

A

T

K

S

A

W

P

P

-

N

M

K

V

V

M

L

D

S

R

G

N

D

F

L

K

S

P

P

G

A

F

F

R

M

I

I

D

D

L

L

H

A

I

H

K
|
K

D

D

L

L

A

G

N

I

A

A

L

L

D

D

T

V

S

A

H

N

G

Q

V

L

G

H

A

V

Q

P

L

M

P

P

L

L

T

G

A

A

V

S

M

R

E

E

M

V

M

Y

Q

S

A

Q

L

A

R

R

A

A

D

A

G

G

L

R

G

G

T

R

A

Q

D

D

H

W

S

S

A

A

L

I

QM 11:12 (≠ IM 10:11) binding NAD(+)
D31 (= D30) binding NAD(+)
L65 (= L64) binding NAD(+)
T96 (≠ S95) binding NAD(+)
G122 (≠ S121) mutation to S: 25-fold decrease in catalytic efficiency with SLA as substrate. 5-fold decrease in catalytic efficiency with NADH as substrate.
R123 (≠ G122) binding 2,3-dihydroxypropane-1-sulfonate; mutation to G: 130-fold decrease in catalytic efficiency with SLA as substrate. 3-fold decrease in catalytic efficiency with NADH as substrate.
T124 (≠ G123) mutation to G: 230-fold decrease in catalytic efficiency with SLA as substrate. 12-fold decrease in catalytic efficiency with NADH as substrate.
NNYMS 174:178 (≠ NQVI- 173:176) binding 2,3-dihydroxypropane-1-sulfonate
K240 (= K238) binding NAD(+)

6smyA Crystal structure of sla reductase yihu from e. Coli with nadh and product dhps
37% identity, 95% coverage: 3:280/294 of query aligns to 3:281/294 of 6smyA

query
sites
6smyA

V

I

G

A

F

F

I

I

G

G

L

L

G

G

I

Q

M

M

G

G

K

S

P

P

M

M

S

A

K

S

N

N

L

L

K

Q

A

Q

G

G

Y

H

S

Q

L

L

V

R

V

V

A

F

D

D

R

V

N

N

P

A

E

E

A

A

I

V

A

R

D

H

V

L

I

V

A

D

A

K

G

G

A

A

E

T

T

P

A

A

S

A

T

N

A

P

K

A

A

Q

I

A

A

A

E

K

Q

D

C

A

D

E

V

F

I

I

T

T

M

M

L

L

P

P

N

N

S

G

P

D

H

L

V

V

K

R

E

N

V

V

A

L

L

F

G

G

E

E

N

N

G

G

I

V

I

C

E

E

G

G

A

L

K

S

P

T

G

D

T

A

V

L

L

V

I

I

D

D

M

M

S

S

S

T

I

I

A

H

P

P

L

L

A

Q

S

T

R

D

E

K

I

L

S

I

E

A

A

D

L

M

K

Q

A

A

K

K

G

G

I

F

D

S

M

M

L

M

D

D

A

V

P

P

V

V

S

G

G

R

G

T

E

S

P

A

K

N

A

A

I

I

D

T

G

G

T

T

L

L

S

L

V

L

M

L

V

A

G

G

D

T

K

A

A

E

I

Q

F

V

D

E

K

R

Y

A

Y

T

D

P

L

I

M

L

K

M

A

A

M

M

A

G

G

S

S

E

V

L

V

I

H

N

T

A

G

G

E

G

I

P

G

G

A

M

G

G

N

I

V

R

T

V

K

K

L

L

A

I

N

N

Q

N

V

Y

I

M

-

S

V

I

A

A

L

L

N

N

I

-

A

A

A

L

M

S

S

A

E

E

A

A

L

A

T

V

L

L

A

C

T

E

K

A

A

L

G

N

V

L

N

P

P

F

D

D

L

V

V

A

Y

V

Q

K

A

V

I

M

R

S

G

G

G

T

L

A

A

A

G

G

S

K

T

G

V

H

L

F

D

T

A

T

K

S

A

W

P

P

-

N

M

K

V

V

M

L

D

S

R

G

N

D

F

L

K

S

P

P

G

A

F
|
F

R

M

I

I

D

D

L

L

H

A

I

H

K

K

D

D

L

L

A

G

N

I

A

A

L

L

D

D

T

V

S

A

H

N

G

Q

V

L

G

H

A

V

Q

P

L

M

P

P

L

L

T

G

A

A

V

S

M

R

E

E

M

V

M

Y

Q

S

A

Q

L

A

R

R

A

A

D

A

G

G

L

R

G

G

T

R

A

Q

D

D

H
x
W

S

S

A

A

L

I

binding (2~{S})-2,3-bis(oxidanyl)propane-1-sulfonic acid: F232 (= F231), W278 (≠ H277)

3pduA Crystal structure of gamma-hydroxybutyrate dehydrogenase from geobacter sulfurreducens in complex with NADP+ (see paper)
33% identity, 94% coverage: 4:280/294 of query aligns to 5:281/287 of 3pduA

query
sites
3pduA

G

G

F

F

I

L

G
|
G

L

L

G
|
G

I
|
I

M
|
M

G

G

K

G

P

P

M

M

S

A

K

A

N

N

L

L

L

V

K

R

A

A

G

G

Y

F

S

D

L

V

V

T

V

V

A

W

D
x
N

R
|
R

N
|
N

P

P

E

A

A

K

I

C

A

A

D

P

V

L

I

V

A

A

A

L

G

G

A

A

E

R

T

Q

A

A

S

S

T

S

A

P

K

A

A

E

I

V

A

C

E

A

Q

A

C

C

D

D

V

I

I

T

I

I

T

A

M
|
M

L
|
L

P
x
A

N

D

S

P

P

A

H
x
A

V

A

K

R

E

E

V

V

A

C

L

F

G

G

E

A

N

N

G

G

I

V

I

L

E

E

G

G

A

I

K

G

P

G

G

G

T

R

V

G

L

Y

I

I

D

D

M

M

S

S

S
x
T

I

V

A

D

P

D

L

E

A

T

S

S

R

T

E

A

I

I

S

G

E

A

A

A

L

V

K

T

A

A

K

R

G

G

I

G

D

R

M

F

L

L

D

E

A

A

P

P

V
|
V

S

S

G
|
G

G
x
T

E

K

P

K

K

P

A

A

I

E

D

D

G

G

T

T

L

L

S

I

V

I

M

L

V

A

G

A

G

G

D

D

K

Q

A

S

I

L

F

F

D

T

K

D

Y

A

Y

G

D

P

L

A

M

F

K

A

A

A

M

L

A

G

G

K

S

K

V

C

V

L

H

H

T

L

G

G

E

E

I

V

G

G

A

Q

G

G

N

A

V

R

T

M

K
|
K

L

L

A

V

N

V

Q

N

V

M

I

I

V

M

A

G

L

Q

N

M

I

M

A

T

A

A

M

L

S

G

E

E

A

G

L

M

T

A

L

L

A

G

T

R

K

N

A

C

G

G

V

L

N

D

P

G

D

G

L

Q

V

L

Y

L

Q

E

A

V

I

L

R

D

G

A

G

G

L

A

A

M

G

A

S

N

T

P

V

M

L

F

D

K

A

G

K

K

A

G

P

Q

M

M

V

L

M

L

D

S

R

G

N

E

F

F

K

P

P

T

G
x
S

F
|
F

R
x
P

I

L

D

K

L
x
H

H

M

I

Q

K
|
K

D

D

L

L

A
x
R

N

L

A

A

L

V

D
x
E

T

L

S

G

H

D

G
x
R

V

L

G

G

A

Q

Q

P

L

L

P

H

L

G

T

A

A

A

A

T

V

A

M

N

E

E

M

S

M

F

Q

K

A

R

L

A

R

R

A

A

D

A

G

G

L

H

G

A

T

D

A

E

D

D

H

F

S

A

A

A

L

V

binding glycerol: R242 (≠ A241), E246 (≠ D245), E246 (≠ D245), R250 (≠ G249)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G7), G10 (= G9), I11 (= I10), M12 (= M11), N31 (≠ D30), R32 (= R31), N33 (= N32), M64 (= M63), L65 (= L64), A66 (≠ P65), A70 (≠ H69), T96 (≠ S95), V121 (= V120), G123 (= G122), T124 (≠ G123), K171 (= K170), S231 (≠ G230), F232 (= F231), P233 (≠ R232), H236 (≠ L235), K239 (= K238)

Q9I5I6 NAD-dependent L-serine dehydrogenase; L-serine 3-dehydrogenase (NAD(+)); EC 1.1.1.387 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
39% identity, 81% coverage: 2:240/294 of query aligns to 3:248/298 of Q9I5I6

query
sites
Q9I5I6

K
x
Q

V
x
I

G
x
A

F
|
F

I
|
I

G
|
G

L
|
L

G
|
G

I
x
H

M
|
M

G
|
G

K
x
A

P
|
P

M
|
M

S
x
A

K
x
T

N
|
N

L
|
L

K
|
K

A
|
A

G
|
G

Y
|
Y

S
x
L

L
|
L

V
x
N

V
|
V

A
x
F

D
|
D

R

L

N

V

P

Q

E

S

A

A

I

V

A

D

D

G

V

L

I

V

A

A

G

G

A

A

E

S

T

A

A

A

S

R

T

S

A

A

K

R

A

D

I

A

A

V

E

Q

Q

G

C

A

D

D

V

V

I

V

I

I

T

S

M

M

L

L

P
|
P

N

A

S

S

P

Q

H

H

V

V

K

E

E

G

V

L

A

Y

L

L

G

D

E

D

N

D

G

G

I

L

E

A

G

H

A

I

K

A

P

P

G

G

T

T

V

L

L

V

I

L

D

E

M

C

S

S

S
x
T

I

I

A

A

P

P

L

T

A

S

S

A

R

R

E

K

I

I

S

H

E

A

A

A

L

A

K

R

A

E

K

R

G

G

I

L

D

A

M

M

L

L

D

D

A

A

P

P

V

V

S
|
S

G

G

E

T

P

A

K

G

A

A

I

A

D

A

G

G

T

T

L

L

S

T

V

F

M

M

V

V

G

G

D

D

K

A

A

E

I

A

F

L

D

E

K

K

Y

A

Y

R

D

P

L

L

M

F

K

E

A

A

M

M

A

G

G

R

S

N

V

I

V

F

H

H

T

A

G

G

E

P

I

D

G

G

A

A

G

G

N

Q

V

V

T

A

K
|
K

L

V

A

C

N

N

Q
x
N

V

Q

I

L

V

L

A

A

L

V

N

L

I

M

A

I

A

G

M

T

S

A

E

E

A

A

L

M

T

A

L

L

A

G

T

V

K

A

A

N

G

G

V

L

N

E

P

A

D

K

L

V

V

L

Y

A

Q

E

A

I

I

M

R

R

G

R

G

S

L

S

A

G

G

G

S

N

T
x
W

V

A

L

L

D

E

A

V

K
x
Y

A

N

P

P

M

W

-

P

-

G

V

V

M

M

D

E

-

N

-

A

-

P

-

A

-

S

R

R

N

D

F

Y

K

S

P

G

G

G

F

F

R

M

I

A

D

Q

L

L

H

M

I

A

K
|
K

D
|
D

L

L

P66 (= P65) binding NAD(+)
T96 (≠ S95) binding NAD(+); mutation to A: Almost abolished activity.
S122 (= S121) mutation to A: Strongly reduced activity.
K171 (= K170) active site
N175 (≠ Q174) mutation to A: Strongly reduced activity.
W214 (≠ T213) mutation to A: Almost abolished activity.
Y219 (≠ K218) mutation to A: Strongly reduced activity.
K246 (= K238) binding NAD(+); mutation to A: Almost abolished activity.
D247 (= D239) mutation to A: Almost abolished activity.

Sites not aligning to the query:

2:31 binding NAD(+)

2uyyA Structure of the cytokine-like nuclear factor n-pac
29% identity, 96% coverage: 2:283/294 of query aligns to 8:287/292 of 2uyyA

query
sites
2uyyA

K

K

V

I

G

G

F

F

I

L

G

G

L

L

G
|
G

I
x
L

M
|
M

G

G

K

S

P

G

M

I

S

V

K

S

N

N

L

L

K

K

A

M

G

G

Y

H

S

T

L

V

V

T

V

V

A

W

D
x
N

R
|
R

N
x
T

P

A

E

E

A

K

I

C

A

D

D

L

V

F

I

I

A

Q

A

E

G

G

A

A

E

R

T

L

A

G

S

R

T

T

A

P

K

A

A

E

I

V

A

V

E

S

Q

T

C

C

D

D

V

I

I

T

I

F

T

A

M

C

L
x
V

P
x
S

N

D

S

P

P

K

H
x
A

V

A

K

K

E

D

V

L

A

V

L

L

G

G

E

P

N

S

G

G

I

V

I

L

E

Q

G

G

A

I

K

R

P

P

G

G

T

K

V

C

L

Y

I

V

D

D

M

M

S

S

S
x
T

I

V

A

D

P

A

L

D

A

T

S

V

R

T

E

E

I

L

S

A

E

Q

A

V

L

I

K

V

A

S

K

R

G

G

I

G

D

R

M

F

L

L

D

E

A

A

P

P

V

V

S

S

G

G

G

N

E

Q

P

Q

K

L

A

S

I

N

D

D

G

G

T

M

L

L

S

V

V

I

M

L

V

A

G

A

G

G

D

D

K

R

A

G

I

L

F

Y

D

E

K

D

Y

C

Y

S

D

S

L

C

M

F

K

Q

A

A

M

M

A

G

G

K

S

T

V

S

V

F

H

F

T

L

G

G

E

E

I

V

G

G

A

N

G

A

N

A

V

K

T

M

K

M

L

L

A

I

N

V

Q

N

V

M

I

V

V

Q

A

G

L

S

N

F

I

M

A

A

A

T

M

I

S

A

E

E

A

G

L

L

T

T

L

L

A

A

T

Q

K

V

A

T

G

G

V

Q

N

S

P

Q

D

Q

L

T

V

L

Y

L

Q

D

A

I

I

L

R

N

G

Q

G

G

L

Q

A

L

G

A

S

S

T

I

V

F

L

L

D

D

A

Q

K

K

A

C

P

Q

M

N

V

I

M

L

D

Q

R

G

N

N

F

F

K

K

P

P

G

D

F
|
F

R
x
Y

I

L

D

K

L
x
Y

H

I

I

Q

K
|
K

D

D

L

L

A

R

N

L

A

A

L

I

D

A

T

L

S

G

H

D

G

A

V

V

G

N

A

H

Q

P

L

T

P

P

L

M

T

A

A

A

V

A

M

N

E

E

M

V

M

Y

Q

K

A

-

L

-

R

R

A

A

D

K

G

A

L

L

G

D

T

Q

A

S

D

D

H

N

S

D

A

M

L

S

A

A

C

V

Y

Y

binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4s)-3,4-dihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: G15 (= G9), L16 (≠ I10), M17 (= M11), N36 (≠ D30), R37 (= R31), T38 (≠ N32), V70 (≠ L64), S71 (≠ P65), A75 (≠ H69), T101 (≠ S95), F237 (= F231), Y238 (≠ R232), Y241 (≠ L235), K244 (= K238)

Q49A26 Cytokine-like nuclear factor N-PAC; NPAC; 3-hydroxyisobutyrate dehydrogenase-like protein; Glyoxylate reductase 1 homolog; Nuclear protein NP60; Nuclear protein of 60 kDa; Nucleosome-destabilizing factor; hNDF; Putative oxidoreductase GLYR1 from Homo sapiens (Human) (see 3 papers)
29% identity, 96% coverage: 2:283/294 of query aligns to 269:548/553 of Q49A26

query
sites
Q49A26

K

K

V

I

G
|
G

F
|
F

I
x
L

G
|
G

L
|
L

G
|
G

I
x
L

M
|
M

G
|
G

K
x
S

P
x
G

M
x
I

S
x
V

K
x
S

N
|
N

L

L

K

K

A

M

G

G

Y

H

S

T

L

V

V

T

V

V

A

W

D

N

R

R

N

T

P

A

E

E

A

K

I

C

A

D

D

L

V

F

I

I

A

Q

A

E

G

G

A

A

E

R

T

L

A

G

S

R

T

T

A

P

K

A

A

E

I

V

A

V

E

S

Q

T

C

C

D

D

V

I

I

T

I

F

T

A

M

C

L

V

P

S

N

D

S

P

P

K

H

A

V

A

K

K

E

D

V

L

A

V

L

L

G

G

E

P

N

S

G

G

I

V

I

L

E

Q

G

G

A

I

K

R

P

P

G

G

T

K

V

C

L

Y

I

V

D

D

M

M

S

S

S
x
T

I

V

A

D

P

A

L

D

A

T

S

V

R

T

E

E

I

L

S

A

E

Q

A

V

L

I

K

V

A

S

K

R

G

G

I

G

D

R

M

F

L

L

D

E

A

A

P

P

V

V

S

S

G

G

G

N

E

Q

P

Q

K

L

A

S

I

N

D

D

G

G

T

M

L

L

S

V

V

I

M

L

V

A

G

A

G

G

D

D

K

R

A

G

I

L

F

Y

D

E

K

D

Y

C

Y

S

D

S

L

C

M

F

K

Q

A

A

M

M

A

G

G

K

S

T

V

S

V

F

H

F

T

L

G

G

E

E

I

V

G

G

A

N

G

A

N

A

V

K

T

M

K
x
M

L

L

A

I

N

V

Q

N

V

M

I

V

V

Q

A

G

L

S

N

F

I

M

A

A

A

T

M

I

S

A

E

E

A

G

L

L

T

T

L

L

A

A

T

Q

K

V

A

T

G

G

V

Q

N

S

P

Q

D

Q

L

T

V

L

Y

L

Q

D

A

I

I

L

R

N

G

Q

G

G

L

Q

A

L

G

A

S

S

T

I

V

F

L

L

D

D

A

Q

K

K

A

C

P

Q

M

N

V

I

M

L

D

Q

R

G

N

N

F

F

K

K

P
|
P

G

D

F

F

R

Y

I

L

D

K

L

Y

H

I

I

Q

K
|
K

D

D

L

L

A

R

N

L

A

A

L

I

D

A

T

L

S

G

H

D

G

A

V

V

G

N

A

H

Q

P

L

T

P

P

L

M

T

A

A

A

V

A

M

N

E

E

M

V

M

Y

Q

K

A

-

L

-

R

R

A

A

D

K

G

A

L

L

G

D

T

Q

A

S

D

D

H

N

S

D

A

M

L

S

A

A

C

V

Y

Y

271:285 (vs. 4:18, 53% identical) binding NAD(+)
T362 (≠ S95) binding NAD(+)
M437 (≠ K170) mutation to K: Loss of tetramerization and protein stability.; mutation to N: No effect on tetramerization or protein stability.
P496 (= P229) to L: decreased interaction with GATA4; decreased synergistic activation of GATA4 target genes transcription; detrimental effect on cardiomyocyte differentiation
K505 (= K238) binding NAD(+)

Sites not aligning to the query:

214 D→A: Slightly reduced stimulation of KDM1B demethylase activity, but normal KDM1B-binding.
214:217 Interaction with histone H3
216 H→A: Slightly reduced stimulation of KDM1B demethylase activity, but normal KDM1B-binding.
216:225 Interaction with KDM1B
217 Required to promote KDM1B demethylase activity toward histone H3K4me1 and H3K4me2; F→A: Abolished stimulation of KDM1B demethylase activity, reduced affinity for histone H3 of the dimer with KDM1B, but normal KDM1B-binding.
219 H→A: Impaired KDM1B-binding and abolished stimulation of KDM1B demethylase activity; when associated with A-223.
220:222 FLL→AAA: Impaired KDM1B-binding and abolished stimulation of KDM1B demethylase activity.
223 S→A: Impaired KDM1B-binding and abolished stimulation of KDM1B demethylase activity; when associated with A-219.

3q3cA Crystal structure of a serine dehydrogenase from pseudomonas aeruginosa pao1 in complex with NAD (see paper)
39% identity, 81% coverage: 2:240/294 of query aligns to 2:246/294 of 3q3cA

query
sites
3q3cA

K

Q

V

I

G

A

F

F

I

I

G

G

L

L

G
|
G

I
x
H

M
|
M

G

G

K

A

P

P

M

M

S

A

K

T

N

N

L

L

K

K

A

A

G

G

Y

Y

S

L

L

L

V

N

V

V

A
x
F

D
|
D

R
x
L

N

V

P

Q

E

S

A

A

I

V

A

D

D

G

V

L

I

V

A

A

G

G

A

A

E

S

T

A

A

A

S

R

T

S

A

A

K

R

A

D

I

A

A

V

E

Q

Q

G

C

A

D

D

V

V

I

V

I

I

T

S

M
|
M

L
|
L

P
|
P

N

A

S

S

P

Q

H

H

V

V

K

E

E

G

V

L

A

Y

L

L

G

-

E

D

N

D

G

G

I

L

E

A

G

H

A

I

K

A

P

P

G

G

T

T

V

L

L

V

I

L

D

E

M

C

S

S

S
x
T

I

I

A

A

P

P

L

T

A

S

S

A

R

R

E

K

I

I

S

H

E

A

A

A

L

A

K

R

A

E

K

R

G

G

I

L

D

A

M

M

L

L

D

D

A

A

P

P

V
|
V

S

S

G
|
G

G

G

E

T

P

A

K

G

A

A

I

A

D

A

G

G

T

T

L

L

S

T

V

F

M

M

V

V

G

G

D

D

K

A

A

E

I

A

F

L

D

E

K

K

Y

A

Y

R

D

P

L

L

M

F

K

E

A

A

M

M

A

G

G

R

S

N

V

I

V

F

H

H

T

A

G

G

E

P

I

D

G

G

A

A

G

G

N

Q

V

V

T

A

K

K

L

V

A

C

N

N

Q

N

V

Q

I

L

V

L

A

A

L

V

N

L

I

M

A

I

A

G

M

T

S

A

E

E

A

A

L

M

T

A

L

L

A

G

T

V

K

A

A

N

G

G

V

L

N

E

P

A

D

K

L

V

V

L

Y

A

Q

E

A

I

I

M

R

R

G

R

G

S

L

S

A

G

G

G

S

N

T

W

V

A

L

L

D

E

A

V

K

Y

A

N

P

P

M

W

-

P

-

G

V

V

M

M

D

E

-

N

-

A

-

P

-

A

-

S

R

R

N

D

F

Y

K

S

P

G

G

G

F
|
F

R

M

I

A

D

Q

L

L

H

M

I

A

K
|
K

D

D

L

L

binding nicotinamide-adenine-dinucleotide: G9 (= G9), H10 (≠ I10), M11 (= M11), F29 (≠ A29), D30 (= D30), L31 (≠ R31), M63 (= M63), L64 (= L64), P65 (= P65), T94 (≠ S95), V119 (= V120), G121 (= G122), F237 (= F231), K244 (= K238)

3obbA Crystal structure of a possible 3-hydroxyisobutyrate dehydrogenase from pseudomonas aeruginosa pao1 (see paper)
39% identity, 81% coverage: 2:240/294 of query aligns to 3:247/295 of 3obbA

query
sites
3obbA

K

Q

V

I

G

A

F

F

I

I

G

G

L

L

G

G

I

H

M

M

G

G

K

A

P

P

M

M

S

A

K

T

N

N

L

L

K

K

A

A

G

G

Y

Y

S

L

L

L

V

N

V

V

A

F

D

D

R

L

N

V

P

Q

E

S

A

A

I

V

A

D

D

G

V

L

I

V

A

A

G

G

A

A

E

S

T

A

A

A

S

R

T

S

A

A

K

R

A

D

I

A

A

V

E

Q

Q

G

C

A

D

D

V

V

I

V

I

I

T

S

M

M

L

L

P

P

N

A

S

S

P

Q

H

H

V

V

K

E

E

G

V

L

A

Y

L

L

G

D

E

D

N

D

G

G

I

L

E

A

G

H

A

I

K

A

P

P

G

-

T

T

V

L

L

V

I

L

D

E

M

C

S

S

S

T

I

I

A

A

P

P

L

T

A

S

S

A

R

R

E

K

I

I

S

H

E

A

A

A

L

A

K

R

A

E

K

R

G

G

I

L

D

A

M

M

L

L

D

D

A

A

P

P

V

V

S

S

G
|
G

E

T

P

A

K

G

A

A

I

A

D

A

G

G

T

T

L

L

S

T

V

F

M

M

V

V

G

G

D

D

K

A

A

E

I

A

F

L

D

E

K

K

Y

A

Y

R

D

P

L

L

M

F

K

E

A

A

M

M

A

G

G

R

S

N

V

I

V

F

H

H

T

A

G

G

E

P

I

D

G

G

A

A

G

G

N

Q

V

V

T

A

K
|
K

L

V

A

C

N

N

Q
x
N

V

Q

I

L

V

L

A

A

L

V

N

L

I

M

A

I

A

G

M

T

S

A

E

E

A

A

L

M

T

A

L

L

A

G

T

V

K

A

A

N

G

G

V

L

N

E

P

A

D

K

L

V

V

L

Y

A

Q

E

A

I

I

M

R

R

G

R

G

S

L

S

A

G

G

G

S

N

T
x
W

V

A

L

L

D

E

A

V

K
x
Y

A

N

P

P

M

W

-

P

-

G

V

V

M

M

D

E

-

N

-

A

-

P

-

A

-

S

R

R

N

D

F

Y

K

S

P

G

G

G

F
|
F

R

M

I

A

D

Q

L

L

H

M

I

A

K

K

D

D

L

L

binding 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid: G122 (= G122), G123 (= G123), K170 (= K170), N174 (≠ Q174), W213 (≠ T213), Y218 (≠ K218), F238 (= F231)

Q922P9 Cytokine-like nuclear factor N-PAC; NPAC; Glyoxylate reductase 1 homolog; Nuclear protein NP60; Putative oxidoreductase GLYR1 from Mus musculus (Mouse) (see paper)
29% identity, 96% coverage: 2:283/294 of query aligns to 268:541/546 of Q922P9

query
sites
Q922P9

K

K

V

I

G

G

F

F

I

L

G

G

L

L

G

G

I

L

M

M

G

G

K

S

P

G

M

I

S

V

K

S

N

N

L

L

K

K

A

M

G

G

Y

H

S

T

L

V

V

T

V

V

A

W

D

N

R

R

N

T

P

A

E

E

A

K

I

-

A

-

D

-

V

-

I

-

A

-

A

E

G

G

A

A

E

R

T

L

A

G

S

R

T

T

A

P

K

A

A

E

I

V

A

V

E

S

Q

T

C

C

D

D

V

I

I

T

I

F

T

A

M

C

L

V

P

S

N

D

S

P

P

K

H

A

V

A

K

K

E

D

V

L

A

V

L

L

G

G

E

P

N

S

G

G

I

V

I

L

E

Q

G

G

A

I

K

R

P

P

G

G

T

K

V

C

L

Y

I

V

D

D

M

M

S

S

S

T

I

V

A

D

P

A

L

D

A

T

S

V

R

T

E

E

I

L

S

A

E

Q

A

V

L

I

K

V

A

S

K

R

G

G

I

G

D

R

M

F

L

L

D

E

A

A

P

P

V

V

S

S

G

G

G

N

E

Q

P

Q

K

L

A

S

I

N

D

D

G

G

T

M

L

L

S

V

V

I

M

L

V

A

G

A

G

G

D

D

K

R

A

G

I

L

F

Y

D

E

K

D

Y

C

Y

S

D

S

L

C

M

F

K

Q

A

A

M

M

A

G

G

K

S

T

V

S

V

F

H

F

T

L

G

G

E

E

I

V

G

G

A

N

G

A

N

A

V

K

T

M

K

M

L

L

A

I

N

V

Q

N

V

M

I

V

V

Q

A

G

L

S

N

F

I

M

A

A

A

T

M

I

S

A

E

E

A

G

L

L

T

T

L

L

A

A

T

Q

K

V

A

T

G

G

V

Q

N

S

P

Q

D

Q

L

T

V

L

Y

L

Q

D

A

I

I

L

R

N

G

Q

G

G

L

Q

A

L

G

A

S

S

T

I

V

F

L

L

D

D

A

Q

K

K

A

C

P

Q

M

N

V

I

M

L

D

Q

R

G

N

N

F

F

K

K

P
|
P

G

D

F

F

R

Y

I

L

D

K

L

Y

H

I

I

Q

K

K

D

D

L

L

A

R

N

L

A

A

L

I

D

A

T

L

S

G

H

D

G

A

V

V

G

N

A

H

Q

P

L

T

P

P

L

M

T

A

A

A

V

A

M

N

E

E

M

V

M

Y

Q

K

A

-

L

-

R

R

A

A

D

K

G

A

L

L

G

D

T

Q

A

S

D

D

H

N

S

D

A

M

L

S

A

A

C

V

Y

Y

P489 (= P229) mutation to L: Mutant animals are born at expected Mendelian ratios. 54% mutants display postnatal lethality between days 0 and 1. They show centricular septal defects.

5y8iA Mycobacterium tuberculosis 3-hydroxyisobutyrate dehydrogenase (mthibadh) + (s)-3-hydroxyisobutyrate (s-hiba) (see paper)
33% identity, 91% coverage: 3:270/294 of query aligns to 3:274/292 of 5y8iA

query
sites
5y8iA

V

I

G

A

F

F

I

L

G

G

L

L

G

G

I

N

M

M

G

G

K

A

P

P

M

M

S

S

K

A

N

N

L

L

L

V

K

G

A

A

G

G

Y

H

S

V

L

V

V

R

V

G

A

F

D

D

R

P

N

A

P

P

E

T

A

A

I

A

A

S

D

G

V

A

I

A

A

A

A

H

G

G

A

V

E

A

T

V

A

F

S

R

T

S

A

A

K

P

A

E

I

A

A

V

E

A

Q

E

C

A

D

D

V

V

I

V

I

I

T

T

M

M

L

L

P

P

N

T

S

G

P

E

H

V

V

V

K

R

E

R

V

C

A

-

L

-

G

-

E

Y

N

T

G

D

I

V

I

L

E

A

G

A

A

A

K

R

P

P

G

A

T

T

V

L

L

F

I

I

D

D

M

S

S

S

S

T

I

I

A

S

P

V

L

T

A

D

S

A

R

R

E

E

I

V

S

H

E

A

A

L

L

A

K

E

A

S

K

H

G

G

I

M

D

L

M

Q

L

L

D

D

A

A

P

P

V

V

S

S

G

G

E

V

P

K

K

G

A

A

I

A

D

A

G

A

T

T

L

L

S

A

V

F

M

M

V

V

G

G

D

D

K

E

A

S

I

T

F

L

D

R

K

R

Y

A

Y

R

D

P

L

V

M

L

K
x
E

A

P

M

M

A
|
A

G

G

S
x
K

V
x
I

V

I

H

H

T

C

G

G

E

A

I

A

G

G

A

A

G

G

N

Q

V

A

T

A

K

K

L

V

A

C

N

N

Q

N

V

M

I

V

V

L

A

A

L

V

N

Q

I

Q

A

I

A

A

M

I

S

A

E

E

A

A

L

F

T

V

L

L

A

A

T

E

K

K

A

L

G

G

V

L

N

S

P

A

D

Q

L

S

V

L

Y

F

Q

D

A

V

I

I

R

T

G

G

G

A

L

T

A

G

G

N

S

C

T

W

V

A

L

V

D

H

A

T

K

N

A

C

P

P

M

V

-

P

-

G

-

P

-

V

-

P

-

T

-

S

V

P

M

A

D

N

R

N

N

D

F

F

K

K

P

P

G

G

F

F

R

S

I

T

D

A

L

L

H

M

I

N

K

K

D

D

L

L

A

G

N

L

A

A

L

M

D

D

T

A

S

V

H

A

G

A

V

T

G

G

A

A

Q

T

L

A

P

P

L

L

T

G

A

S

A

H

V

A

M

A

E

D

M

I

M

Y

Q

A

A

K

L

F

R

A

A

A

D

D

binding (2~{S})-2-methylpentanedioic acid: E148 (≠ K151), A151 (= A154), K153 (≠ S156), I154 (≠ V157)

5y8lB Mycobacterium tuberculosis 3-hydroxyisobutyrate dehydrogenase (mthibadh) + NAD +(s)-3-hydroxyisobutyrate (s-hiba) (see paper)
33% identity, 91% coverage: 3:270/294 of query aligns to 4:275/290 of 5y8lB

query
sites
5y8lB

V

I

G

A

F

F

I

L

G
|
G

L

L

G
|
G

I
x
N

M
|
M

G

G

K

A

P

P

M

M

S

S

K

A

N

N

L

L

L

V

K

G

A

A

G

G

Y

H

S

V

L

V

V

R

V

G

A
x
F

D
|
D

R
x
P

N

A

P

P

E

T

A

A

I

A

A

S

D

G

V

A

I

A

A

A

A

H

G

G

A

V

E

A

T

V

A

F

S

R

T

S

A

A

K

P

A

E

I

A

A

V

E

A

Q

E

C

A

D

D

V

V

I

V

I

I

T

T

M
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M

L
|
L

P

P

N

T

S

G

P

E

H

V

V

V

K

R

E

R

V

C

A

-

L

-

G

-

E

Y

N

T

G

D

I

V

I

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E

A

G

A

A

A

K

R

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P

G

A

T

T

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L

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I

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x
T

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I

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T

A

D

S

A

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R

E

E

I

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S

H

E

A

A

L

L

A

K

E

A

S

K

H

G

G

I

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D

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M

Q

L

L

D

D

A

A

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S

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G

E

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K

K

G

A

A

I

A

D

A

G

A

T
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T

L

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A

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F

M

M

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V

G

G

D

D

K

E

A

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K

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Y

A

Y

R

D

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x
E

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A
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A

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G

S
x
K

V

I

H

H

T

C

G

G

E

A

I

A

G

G

A

A

G

G

N

Q

V

A

T

A

K
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K

L

V

A

C

N

N

Q

N

V

M

I

V

V

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A

A

L

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N

Q

I

Q

A

I

A

A

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A

E

E

A

A

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F

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V

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L

A

A

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K

A

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G

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D

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I

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G

G

A

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A

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G

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x
W

V

A

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V

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H

A

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K

N

A

C

P

P

M

V

-

P

-

G

-

P

-

V

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P

-

T

-

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P

M

A

D

N

R

N

N

D

F

F

K

K

P

P

G

G

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F

R

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D

A

L
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L

H

M

I

N

K
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K

D

D

L

L

A

G

N

L

A

A

L

M

D

D

T

A

S

V

H

A

G

A

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G

G

A

A

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T

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P

L

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A

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D

binding (2~{S})-2-methylpentanedioic acid: T129 (= T131), E149 (≠ K151), A152 (= A154), G153 (= G155), G153 (= G155), K154 (≠ S156)
binding (2S)-2-methyl-3-oxidanyl-propanoic acid: S119 (= S121), G120 (= G122), W211 (≠ T213), F236 (= F231)
binding nicotinamide-adenine-dinucleotide: G8 (= G7), G10 (= G9), N11 (≠ I10), M12 (= M11), F30 (≠ A29), D31 (= D30), P32 (≠ R31), M64 (= M63), L65 (= L64), T93 (≠ S95), G121 (= G123), K168 (= K170), L240 (= L235), K243 (= K238)

5y8kA Mycobacterium tuberculosis 3-hydroxyisobutyrate dehydrogenase (mthibadh) + l-serine (see paper)
33% identity, 91% coverage: 3:270/294 of query aligns to 4:275/290 of 5y8kA

query
sites
5y8kA

V

I

G

A

F

F

I

L

G

G

L

L

G

G

I

N

M

M

G

G

K

A

P

P

M

M

S

S

K

A

N

N

L

L

L

V

K

G

A

A

G

G

Y

H

S

V

L

V

V

R

V

G

A

F

D

D

R

P

N

A

P

P

E

T

A

A

I

A

A

S

D

G

V

A

I

A

A

A

A

H

G

G

A

V

E

A

T

V

A

F

S

R

T

S

A

A

K

P

A

E

I

A

A

V

E

A

Q

E

C

A

D

D

V

V

I

V

I

I

T

T

M

M

L

L

P

P

N

T

S

G

P

E

H

V

V

V

K

R

E

R

V

C

A

-

L

-

G

-

E

Y

N

T

G

D

I

V

I

L

E

A

G

A

A

A

K

R

P

P

G

A

T

T

V

L

L

F

I

I

D

D

M

S

S

S

S
x
T

I

I

A

S

P

V

L

T

A

D

S

A

R

R

E

E

I

V

S

H

E

A

A

L

L

A

K

E

A

S

K

H

G

G

I

M

D

L

M

Q

L

L

D

D

A

A

P

P

V

V

S

S

G

G

E

V

P

K

K

G

A

A

I

A

D

A

G

A

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T

L

L

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M

M

V

V

G

G

D

D

K

E

A

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I

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F

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R

Y

A

Y
x
R

D

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L

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x
E

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M

A
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A

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G

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K

V
x
I

V

I

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H

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C

G

G

E

A

I

A

G

G

A

A

G

G

N

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K

L

V

A

C

N

N

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N

V

M

I

V

V

L

A

A

L

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I

Q

A

I

A

A

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I

S

A

E

E

A

A

L

F

T

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L

A

A

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K

A

L

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G

V

L

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A

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S

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L

Y

F

Q

D

A

V

I

I

R

T

G

G

G

A

L

T

A

G

G

N

S

C

T

W

V

A

L

V

D

H

A

T

K

N

A

C

P

P

M

V

-

P

-

G

-

P

-

V

-

P

-

T

-

S

V

P

M

A

D

N

R

N

N

D

F

F

K

K

P

P

G

G

F
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F

R

S

I

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A

L
|
L

H

M

I

N

K
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K

D
|
D

L

L

A

G

N

L

A

A

L

M

D

D

T

A

S

V

H

A

G

A

V

T

G

G

A

A

Q

T

L

A

P

P

L

L

T

G

A

S

A

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V

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M

A

E

D

M

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A

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L

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D

binding (2~{S})-2-methylpentanedioic acid: R145 (≠ Y147), E149 (≠ K151), A152 (= A154), G153 (= G155), I155 (≠ V157)
binding serine: T93 (≠ S95), K168 (= K170), F236 (= F231), L240 (= L235), K243 (= K238), D244 (= D239)

Query Sequence

>17198 FitnessBrowser__Keio:17198
MKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCDVI
ITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDAPV
SGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHTGEIGAGNVTKLANQVIVALN
IAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIKDL
ANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKLAKVEVTR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory