SitesBLAST
Comparing 17200 FitnessBrowser__Keio:17200 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 15 hits to proteins with known functional sites (download)
P0AA76 D-galactonate transporter; D-galactonate/H(+) symporter from Escherichia coli (strain K12) (see paper)
41% identity, 98% coverage: 6:440/444 of query aligns to 5:429/430 of P0AA76
- Y29 (= Y31) binding
- D31 (= D33) mutation to N: Loss of galactonate transport activity.
- R32 (= R34) binding
- Y64 (= Y66) binding
- E118 (= E126) mutation to Q: Loss of galactonate transport activity.
- W358 (= W368) binding
6e9nA E. Coli d-galactonate:proton symporter in the inward open form (see paper)
41% identity, 96% coverage: 14:439/444 of query aligns to 1:409/409 of 6e9nA
6e9oA E. Coli d-galactonate:proton symporter mutant e133q in the outward substrate-bound form (see paper)
38% identity, 96% coverage: 14:439/444 of query aligns to 4:393/393 of 6e9oA
Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
24% identity, 92% coverage: 12:419/444 of query aligns to 66:481/582 of Q9JI12
- R88 (= R34) mutation to A: Impairs synaptic transmission. Abolishes the chloride ion conductance.
- H128 (≠ F59) mutation to A: Greatly lowers L-glutamate transport.
- R184 (= R119) mutation R->A,E,K: Greatly lowers L-glutamate transport.
- E191 (= E126) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
- R322 (≠ I262) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.
7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
23% identity, 89% coverage: 12:408/444 of query aligns to 8:412/452 of 7t3nA
- binding (1s,3r)-1-aminocyclopentane-1,3-dicarboxylic acid: Y77 (= Y66), Y137 (≠ F130), Y165 (= Y158), R264 (≠ I262), F268 (≠ T266), Y269 (≠ W267)
- binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: R12 (= R16), Y13 (= Y17), E152 (= E145), G163 (≠ A156)
Q9Y2C5 Probable small intestine urate exporter; Solute carrier family 17 member 4 from Homo sapiens (Human) (see 2 papers)
28% identity, 82% coverage: 55:419/444 of query aligns to 110:464/497 of Q9Y2C5
- A372 (≠ V329) to T: in dbSNP:rs11754288
Sites not aligning to the query:
- 66 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
- 75 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-66 and A-90.
- 90 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
24% identity, 92% coverage: 35:444/444 of query aligns to 88:489/495 of Q9NRA2
- K136 (= K83) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
- H183 (≠ A134) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
- LL 198:199 (≠ AS 149:150) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
- IL 266:267 (≠ IS 216:217) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
- SS-------LRN 268:272 (≠ ENGAVVDMDHKK 218:229) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
- G328 (= G289) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
- P334 (= P295) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
- G371 (= G331) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172
Sites not aligning to the query:
- 22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
- 39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794
Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
25% identity, 82% coverage: 40:405/444 of query aligns to 68:430/493 of Q03567
- N69 (≠ G41) modified: carbohydrate, N-linked (GlcNAc...) asparagine
Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
22% identity, 92% coverage: 35:444/444 of query aligns to 88:489/495 of Q5Q0U0
- K136 (= K83) mutation to E: Markedly decreases H(+)-coupled sialic acid transporter activity.
- R168 (= R119) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
- E171 (≠ L122) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
- G172 (= G123) mutation to C: Decreases protein levels and alters subcellular localization.
- E175 (= E126) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
- G176 (≠ A127) mutation to C: Decreases protein levels and alters subcellular localization.
- F179 (= F130) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
- P180 (= P131) mutation to C: Decreases protein levels and alters subcellular localization.
- H183 (≠ A134) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
- W186 (≠ V137) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
- SS-------LKN 268:272 (≠ ENGAVVDMDHKK 218:229) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
- P334 (= P295) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
- G371 (= G331) mutation to V: Remains in the endoplasmic reticulum.
Sites not aligning to the query:
- 22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
- 39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.
Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
22% identity, 92% coverage: 35:444/444 of query aligns to 88:489/495 of Q8BN82
- H183 (≠ A134) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.
Sites not aligning to the query:
- 39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.
Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
22% identity, 79% coverage: 16:367/444 of query aligns to 20:359/452 of Q5EXK5
- D82 (= D78) mutation to A: Loss of activity.
- V311 (= V308) mutation to W: Loss of activity.
- D314 (= D311) mutation to A: Loss of activity.
Q9GQQ0 Protein spinster; Protein benchwarmer; Protein diphthong from Drosophila melanogaster (Fruit fly) (see paper)
22% identity, 81% coverage: 14:373/444 of query aligns to 111:448/605 of Q9GQQ0
- E217 (= E126) mutation to K: In bnch(N); leads to storage in yolk spheres during oogenesis and results in widespread accumulation of enlarged lysosomal and late endosomal inclusions.
P77589 3-(3-hydroxy-phenyl)propionate transporter; 3HPP transporter; 3-(3-hydroxy-phenyl)propionate:H(+) symporter; 3HPP:H(+) symporter from Escherichia coli (strain K12) (see paper)
23% identity, 52% coverage: 50:280/444 of query aligns to 47:245/403 of P77589
- D75 (= D78) mutation D->A,E: Lack of 3HPP transport activity.
Sites not aligning to the query:
- 27 E→A: Lack of 3HPP transport activity.; E→D: Slight decrease in 3HPP transport activity.
- 272 A→H: 30% increase in 3HPP transport activity.
- 276 K→D: Lack of 3HPP transport activity.
A2SWM2 Sphingosine-1-phosphate transporter SPNS2; Protein spinster homolog 2; Protein two of hearts from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
22% identity, 57% coverage: 29:281/444 of query aligns to 67:297/504 of A2SWM2
- R153 (= R119) mutation to S: In ko157; displays cardia bifida (2 hearts).
Q8IVW8 Sphingosine-1-phosphate transporter SPNS2; Protein spinster homolog 2 from Homo sapiens (Human) (see 2 papers)
22% identity, 72% coverage: 22:341/444 of query aligns to 107:412/549 of Q8IVW8
- R200 (= R119) mutation to S: Loss of function; does not rescue the cardia bifida phenotype in the morpholino knockdown in zebrafish.
- S319 (≠ F256) natural variant: Missing (in DFNB115; uncertain significance; dbSNP:rs749994718)
Query Sequence
>17200 FitnessBrowser__Keio:17200
MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEVAKELQLSAVSMGYIFS
AFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRF
MLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHV
FTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKL
HYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGF
AGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNNTTLVVMLMALAFFG
KGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNAALVFVG
CSALMAMVCYLFVVGDIKRMELQK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory