SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 17328 FitnessBrowser__Keio:17328 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 18 hits to proteins with known functional sites (download)

P07117 Sodium/proline symporter; Proline carrier; Proline permease; Propionate transporter from Escherichia coli (strain K12) (see 4 papers)
27% identity, 97% coverage: 9:478/483 of query aligns to 8:480/502 of P07117

query
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P07117

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R257 (= R259) mutation to C: Sodium-independent binding affinity for proline.
C281 (≠ L283) mutation to S: Does not affect proline uptake activity. Confers resistance to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
C344 (≠ A338) mutation to S: Small decrease in proline uptake activity. Confers resistance to N-ethylmaleimide. Exhibits low Na(+)-dependent proline binding.
C349 (≠ S343) mutation to S: Does not affect proline uptake activity. Sensitive to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
R376 (≠ A371) mutation R->E,Q: No change in activity.; mutation to K: Loss of activity.

Q9NY91 Probable glucose sensor protein SLC5A4; Solute carrier family 5 member 4 from Homo sapiens (Human) (see paper)
24% identity, 88% coverage: 9:432/483 of query aligns to 32:492/659 of Q9NY91

query
sites
Q9NY91

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E457 (≠ G402) mutation to Q: Confers sugar transport activity not found in the wild-type protein. Increased sensitivity to inhibitor phlorizin.

7vsiA Structure of human sglt2-map17 complex bound with empagliflozin (see paper)
26% identity, 74% coverage: 6:363/483 of query aligns to 6:401/586 of 7vsiA

query
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7vsiA

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G

C

T

G

I

Y

V

L

V
x
K

A

L

I

T

L

P

M

M

F

F

G

L

M

M

H

V

L

M

A

P

G

G

A

M

L

I

G

S

R

R

A

I

V

L

I

Y

P

P

D

D

-

E

-

V

-

A

L

C

T

V

V

V

P

P

D

E

L

V

V

C

-

R

-

R

-

V

-

C

-

G

-

T

-

E

-

V

-

G

-

C

-

S

-

N

-

I

-

A

I

Y

P

P

T

R

L

L

M

V

V

V

K

K

V

L

L

M

P

P

P

N

F

G

A

L

A

R

G

G

I

L

F

M

L

L

A

A

A

V

P

M

M

L

A

A

A

A

I

L

M

M

S

S

T

S

I

L

N

A

A

S

Q

I

L

F

L

N

Q

S

S

S

S

S

A

T

T

L

I

F

I

T

K

M

D

D

L

I

Y

Y

L

T

N

R

I

L

R

R

P

P

D

R

Q

A

M

G

Q

D

N

R

E

E

binding (2S,3R,4R,5S,6R)-2-[4-chloranyl-3-[[4-[(3S)-oxolan-3-yl]oxyphenyl]methyl]phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: N55 (≠ Y56), H60 (≠ S61), G63 (= G64), L64 (≠ G65), T67 (≠ A68), V75 (≠ L79), F78 (≠ I82), E79 (≠ Q83), A82 (= A86), V137 (= V141), V266 (≠ T240), S267 (= S241), Y270 (≠ V244), W271 (≠ L245), K301 (≠ V280)

Sites not aligning to the query:

binding (2S,3R,4R,5S,6R)-2-[4-chloranyl-3-[[4-[(3S)-oxolan-3-yl]oxyphenyl]methyl]phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: 433, 434, 437, 506

P31639 Sodium/glucose cotransporter 2; Na(+)/glucose cotransporter 2; Low affinity sodium-glucose cotransporter; Solute carrier family 5 member 2 from Homo sapiens (Human) (see paper)
26% identity, 74% coverage: 6:363/483 of query aligns to 26:421/672 of P31639

query
sites
P31639

I

I

L

L

P

V

L

I

V

A

A

A

Y

Y

L

F

V

L

V

L

V

V

F

I

G

G

I

V

S

G

V

L

Y

W

A

S

M

M

R

C

K

R

R

T

S

N

T

R

G

G

T

T

F

-

L

V

N

G

E

G

Y

Y

F

F

L

L

G

A

S

G

R

R

S

S

M

M

G

V

G

W

I

W

V

P

L

V

A

G

M

A

T

S

L

L

T

F

A

A

T

S

Y

N

I

I

S

G

A

S

S

G

S

H

F

F

I

V

G

G

G

L

P

A

G

G

A

T

A

G

Y

A

K

A

Y

S

G

G

L

L

G

A

W

-

V

-

L

-

L
x
V

A

A

M

G

I
x
F

Q

E

L

W

P

N

A

A

V

L

W

F

L

V

S

V

L

L

G

-

I

L

L

L

G

G

K

W

K

L

F

F

A

A

I

P

L

V

A

Y

R

L

R

T

Y

A

N

G

A

V

V

I

T

T

L

M

N

P

D

Q

M

Y

L

L

F

R

A

K

R

R

Y

F

Q

G

-

G

S

R

R

R

L

I

L

R

V

L

W

Y

L

L

A

S

S

V

L

L

S

S

L

L

L

F

V

L

A

Y

F

I

V

F

G

T

A

K

M

I

T

S

V
|
V

Q

D

F

M

I

F

G

S

G

G

A

A

R

V

L

F

L

I

E

Q

T

Q

A

A

A

L

G

G

I

W

P

N

-

I

Y

Y

E

A

T

S

G

V

L

I

L

A

I

L

F

L

G

G

I

I

S

T

I

M

A

-

L

I

Y

Y

T

T

A

V

F

T

G

G

G

G

F

L

R

A

A

A

S

L

V

M

L

Y

N

T

D

D

T

T

M

V

Q

Q

G

T

L

F

V

V

M

I

L

L

I

G

G

G

T

A

V

C

V

I

L

L

L

M

I

G

G

Y

V

A

V

F

H

H

A

E

A

V

G

G

G

G

L

Y

S

S

N

G

-

L

-

F

-

D

-

K

-

Y

-

L

-

G

-

A

A

A

V

T

Q

S

T

L

L

T

Q

V

T

S

I

E

D

D

P

P

-

A

-

V

-

G

-

N

-

I

-

S

-

S

-

F

-

C

-

Y

-

R

-

P

-

R

-

P

-

D

-

S

-

Y

Q

H

L

L

V

L

T

R

P

H

Q

P

G

V

A

T

D

G

D

D

I

L

L

P

S

W

P

P

A

A

F

L

M

L

-

L

-

G

-
x
L

-

T

-

I

T

V

S

S

F

G

W

W

V

Y

L

W

V

C

C

S

F

D

G

Q

V

V

I

I

G

-

L

-

P

-

H

-

T

-

A

V

V

Q

R

R

C

C

I

L

S

A

Y

G

K

K

D

S

S

L

K

T

A

H

V

I

H

K

R

A

G

G

I

C

I

I

I

L

G

C

T

G

I

Y

V

L

V

K

A

L

I

T

L

P

M

M

F

F

G

L

M

M

H

V

L

M

A

P

G

G

A

M

L

I

G

S

R

R

A

I

V

L

I

Y

P

P

D

D

-

E

-

V

-

A

L

C

T

V

V

V

P

P

D

E

L

V

V

C

-

R

-

R

-

V

-

C

-

G

-

T

-

E

-

V

-

G

-

C

-

S

-

N

-

I

-

A

I

Y

P

P

T

R

L

L

M

V

V

V

K

K

V

L

L

M

P

P

P

N

F

G

A

L

A

R

G

G

I

L

F

M

L

L

A

A

A

V

P

M

M

L

A

A

A

A

I

L

M

M

S

S

T

S

I

L

N

A

A

S

Q

I

L

F

L

N

Q

S

S

S

S

S

A

T

T

L

I

F

I

T

K

M

D

D

L

I

Y

Y

L

T

N

R

I

L

R

R

P

P

D

R

Q

A

M

G

Q

D

N

R

E

E

V95 (≠ L79) mutation to A: Strong reduction in D-glucose transporter activity. Confers partial resistance to empagliflozin inhibition.
F98 (≠ I82) mutation to A: Slightly decreases D-glucose transporter activity. Abolishes the binding to inhibitor, empagliflozin.
V157 (= V141) mutation to A: Decreases D-glucose transporter activity.
L283 (vs. gap) mutation to M: Strong reduction in D-glucose transporter activity. Confers partial resistance to empagliflozin inhibition.

Sites not aligning to the query:

453 F→A: Slightly decreases D-glucose transporter activity. Greatly reduces the binding to inhibitor, empagliflozin.

7sl8A Cryoem structure of sglt1 at 3.4 a resolution (see paper)
25% identity, 87% coverage: 9:426/483 of query aligns to 13:454/582 of 7sl8A

query
sites
7sl8A

L

I

V

V

A

I

Y

Y

L

F

V

L

V

L

V

V

F

M

G

A

I

V

S

G

V

L

Y

W

A

S

M

M

R

F

K

K

R

R

S

S

T

-

G

-

T

-

F

-

L

-

N

-

E

-

Y

-

F

-

L

-

G

-

S

-

R

-

S

-

M

M

G

V

G
x
W

I

W

V

P

L

I

A

G

M

A

T

S

L

L

T

F

A

A

T

S

Y

N

I

I

S

G

A

S

S

G

S

H

F

F

I

I

G

G

-

L

-

A

G

G

P

T

G

G

A

A

A

A

Y

S

K

G

Y

L

G

A

L

V

G

G

-

G

-

F

-

E

W

W

V

N

L

A

L

L

A

V

M

L

I

L

Q

L

L

V

P

-

A

-

V

-

W

-

L

-

S

-

L

-

G

-

I

-

L

L

G

G

K

W

K

V

F

F

A

V

I

P

L

I

A

Y

R

I

R

K

Y

A

N

G

A

V

V

V

T

T

L

M

N

P

D

E

M

Y

L

L

F

R

A

K

R

R

Y

F

Q

G

-

G

S

Q

R

R

L

I

L

Q

V

V

W

Y

L

L

A

S

S

V

L

L

S

S

L

L

L

F

V

L

A

Y

F

I

V

F

G

T

A

K

M

I

T

S

V

V

Q

D

F

I

I

F

G

S

G

G

A

A

R

I

L

F

L

I

E

N

T

L

A

A

A

L

G

G

I

W

P

N

-

L

Y

Y

E

L

T

S

G

I

L

I

L

L

I

L

F

L

G

A

I

I

S

T

I

-

A

A

L

L

Y

Y

T

T

A

I

F

T

G

G

G

G

F

L

R

A

A

A

S

V

V

I

L

Y

N

T

D

D

T

T

M

L

Q

Q

G

T

L

L

V

I

M

M

L

L

I

I

G

G

T

A

V

L

V

I

L

L

L

M

I

G

G

F

V

A

V

F

H

H

A

E

A

V

G

G

G

G

L

Y

S

D

-

A

-

F

-

M

-

E

-

K

-

Y

-

M

N

K

A

A

V

I

Q

P

T

T

L

I

-

V

-

S

-

D

-

G

-

N

Q

T

T

T

I

F

D

Q

P

E

Q

K

L

C

V

Y

T

T

P

P

Q

R

G

A

A

D

D

S

-

F

-

H

-

I

-

F

-

R

-

D

-

P

-

L

-

T

-

G

D

D

I

L

L

P

S

W

P

P

A

G

F

F

M

I

T

F

S

G

F

L

W

T

V

I

L

L

V

A

C

L

F

W

G

Y

V

W

I

C

G

T

L

D

P

Q

H

V

T

I

A

V

V

Q

R

R

C

C

I

L

S

A

Y

A

K

K

D

N

S

M

K

S

A

H

V

V

H

K

R
x
G

G

G

I

C

I

I

I

L

G

A

T

G

I
x
Y

V

L

V

K

A

L

I

L

L

P

M

M

F

F

G

I

M

M

H

V

L

M

A

P

G

G

A

M

L

I

G

S

R

R

A

I

V

L

I

F

P

P

D

D

-

K

-

V

-

A

L

C

T

V

V

V

P

P

-

S

-

E

-

C

-

E

-

K

-

Y

-

C

-

G

-

T

-

K

-

V

-

G

-

C

-

T

D

N

L

I

V

A

I

Y

P

P

T

T

L

L

M

V

V

V

K

E

V

L

L

M

P

P

P

N

F

G

A

L

A

R

G

G

I

L

F

M

L

L

A

A

A

V

P

M

M

L

A

A

A

A

I

L

M

M

S

S

T

S

I

L

N

T

A

S

Q

I

L

F

L

N

Q

S

S

A

S

S

A

T

T

L

I

F

I

T

K

M

D

D

L

I

Y

Y

L

A

N

K

I

V

R

R

P

K

D

R

Q

A

M

S

Q

E

N

K

E

E

T

L

R

M

L

I

K

V

R

G

M

R

S

L

A

F

V

V

I

L

T

F

L

L

V

V

L

V

G

V

A

S

L

I

L

-

L

-

L

-

A

-

A

A

W

W

K

I

P

P

P

I

E

V

M

Q

I

S

I

A

W

Q

L

S

N

G

L

Q

L

L

A

-

F

F

G

D

G

Y

L

I

E

Q

A

S

V

V

-

S

-

S

F

Y

L

L

W

A

P

P

-

P

-

V

-

A

-

A

-

V

L

F

V

L

L

L

G

A

L

I

Y

F

W

W

E

K

R

R

A

V

N

N

A

E

K

Q

G

G

A

A

L

F

binding cholesterol: W35 (≠ G45), G280 (≠ R271), Y287 (≠ I278)

Sites not aligning to the query:

binding cholesterol: 551, 554, 555, 558

7slaA Cryoem structure of sglt1 at 3.15 angstrom resolution (see paper)
25% identity, 87% coverage: 9:426/483 of query aligns to 14:455/585 of 7slaA

query
sites
7slaA

L

I

V

V

A

I

Y

Y

L

F

V

L

V

L

V

V

F

M

G

A

I

V

S

G

V

L

Y

W

A

S

M

M

R

F

K

K

R

R

S

S

T

-

G

-

T

-

F

-

L

-

N

-

E

-

Y

-

F

-

L

-

G

-

S

-

R

-

S

-

M

M

G

V

G
x
W

I

W

V

P

L

I

A

G

M

A

T

S

L

L

T

F

A

A

T

S

Y

N

I

I

S

G

A

S

S

G

S

H

F

F

I

I

G

G

-

L

-

A

G

G

P

T

G

G

A

A

A

A

Y

S

K

G

Y

L

G

A

L

V

G

G

-

G

-

F

-

E

W

W

V

N

L

A

L

L

A

V

M

L

I

L

Q

L

L

V

P

-

A

-

V

-

W

-

L

-

S

-

L

-

G

-

I

-

L

L

G

G

K

W

K

V

F

F

A

V

I

P

L

I

A

Y

R

I

R

K

Y

A

N

G

A

V

V

V

T

T

L

M

N

P

D

E

M

Y

L

L

F

R

A

K

R

R

Y

F

Q

G

-

G

S

Q

R

R

L

I

L

Q

V

V

W

Y

L

L

A

S

S

V

L

L

S

S

L

L

L

F

V

L

A

Y

F

I

V

F

G

T

A

K

M

I

T

S

V

V

Q

D

F

I

I

F

G

S

G

G

A

A

R

I

L

F

L

I

E

N

T

L

A

A

A

L

G

G

I

W

P

N

-

L

Y

Y

E

L

T

S

G

I

L

I

L

L

I

L

F

L

G

A

I

I

S

T

I

-

A

A

L

L

Y

Y

T

T

A

I

F

T

G

G

G

G

F

L

R

A

A

A

S

V

V

I

L

Y

N

T

D

D

T

T

M

L

Q

Q

G

T

L

L

V

I

M

M

L

L

I

I

G

G

T

A

V

L

V

I

L

L

L

M

I

G

G

F

V

A

V

F

H

H

A

E

A

V

G

G

G

G

L

Y

S

D

-

A

-

F

-

M

-

E

-

K

-

Y

-

M

N

K

A

A

V

I

Q

P

T

T

L

I

-

V

-

S

-

D

-

G

-

N

Q

T

T

T

I

F

D

Q

P

E

Q

K

L

C

V

Y

T

T

P

P

Q

R

G

A

A

D

D

S

-

F

-

H

-

I

-

F

-

R

-

D

-

P

-

L

-

T

-

G

D

D

I

L

L

P

S

W

P

P

A

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binding cholesterol hemisuccinate: W36 (≠ G45), S277 (≠ K267), Y288 (≠ I278)

Sites not aligning to the query:

binding cholesterol hemisuccinate: 554, 558, 561

P11170 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
24% identity, 88% coverage: 9:432/483 of query aligns to 32:492/662 of P11170

query
sites
P11170

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Q

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T

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A

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W

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N

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C255 (≠ A230) modified: Disulfide link with 608
Q457 (vs. gap) mutation to W: Drasticly decreased affinity for glucose and phlorizin.
T460 (≠ L398) mutation to W: Decreased affinity for glucose and phlorizin.

Sites not aligning to the query:

608 modified: Disulfide link with 255

Q9ET37 Solute carrier family 5 member 4A; SGLT3-a from Mus musculus (Mouse) (see paper)
24% identity, 88% coverage: 9:435/483 of query aligns to 32:495/656 of Q9ET37

query
sites
Q9ET37

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T

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A

A

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L

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x
E

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P

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P

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L

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A

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C

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R

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A

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N

A

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G

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M

E457 (≠ G402) mutation to Q: Confers sodium-dependent sugar transport activity not found in the wild type protein.

7wmvA Structure of human sglt1-map17 complex bound with lx2761 (see paper)
25% identity, 87% coverage: 9:426/483 of query aligns to 15:469/602 of 7wmvA

query
sites
7wmvA

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Y
x
N

I

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S

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x
H

F

F

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G

G

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x
L

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T

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A

A

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G

Y

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x
I

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G

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F

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x
E

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W

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A

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L

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W

L

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F

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binding N-[2-(dimethylamino)ethyl]-2-methyl-2-[4-[4-[[2-methyl-5-[(2S,3R,4R,5S,6R)-6-methylsulfanyl-3,4,5-tris(oxidanyl)oxan-2-yl]phenyl]methyl]phenyl]butanoylamino]propanamide: N61 (≠ Y56), H66 (≠ S61), L70 (vs. gap), I81 (≠ L74), F84 (vs. gap), E85 (vs. gap), A88 (≠ L78), D256 (= D232), L257 (≠ I233), M266 (≠ F242), L269 (= L245), T270 (≠ V246), Y273 (≠ G249), W274 (≠ V250), K304 (≠ V280), F436 (≠ I392), D437 (≠ I393), Q440 (vs. gap)

Sites not aligning to the query:

binding N-[2-(dimethylamino)ethyl]-2-methyl-2-[4-[4-[[2-methyl-5-[(2S,3R,4R,5S,6R)-6-methylsulfanyl-3,4,5-tris(oxidanyl)oxan-2-yl]phenyl]methyl]phenyl]butanoylamino]propanamide: 508

P13866 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Homo sapiens (Human) (see 6 papers)
25% identity, 87% coverage: 9:426/483 of query aligns to 32:486/664 of P13866

query
sites
P13866

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