SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 17519 b3458 leucine transporter subunit (NCBI) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1uskA L-leucine-binding protein with leucine bound (see paper)
100% identity, 93% coverage: 24:368/369 of query aligns to 1:345/345 of 1uskA

query
sites
1uskA

D

D

D

D

I

I

K

K

V

V

A

A

V

V

V

V

G

G

A

A

M

M

S

S

G

G

P

P

I

I

A

A

Q

Q

W
|
W

G

G

D

D

M

M

E

E

F

F

N

N

G

G

A

A

R

R

Q

Q

A

A

I

I

K

K

D

D

I

I

N

N

A

A

K

K

G

G

G

G

I

I

K

K

G

G

D

D

K

K

L

L

V

V

G

G

V

V

E

E

Y

Y

D

D

D

D

A

A

C

C

D

D

P

P

K

K

Q

Q

A

A

V

V

A

A

V

V

A

A

N

N

K

K

I

I

V

V

N

N

D

D

G

G

I

I

K

K

Y

Y

V

V

I

I

G

G

H

H

L
|
L

C
|
C

S
|
S

S

S

S

S

T

T

Q

Q

P

P

A

A

S

S

D

D

I

I

Y

Y

E

E

D

D

E

E

G

G

I

I

L

L

M

M

I

I

S

S

P

P

G
|
G

A
|
A

T
|
T

N

N

P

P

E

E

L

L

T

T

Q

Q

R

R

G

G

Y

Y

Q

Q

H

H

I

I

M

M

R

R

T

T

A

A

G

G

L

L

D

D

S

S

S

S

Q

Q

G

G

P

P

T

T

A

A

A

A

K

K

Y

Y

I

I

L

L

E

E

T

T

V

V

K

K

P

P

Q

Q

R

R

I

I

A

A

I

I

I

I

H

H

D

D

K

K

Q

Q

Q

Q

Y
|
Y

G

G

E

E

G

G

L

L

A

A

R

R

S

S

V

V

Q

Q

D

D

G

G

L

L

K

K

A

A

A

A

N

N

A

A

N

N

V

V

V

V

F

F

F

F

D

D

G

G

I

I

T

T

A

A

G

G

E

E

K

K

D

D

F

F

S

S

A

A

L

L

I

I

A

A

R

R

L

L

K

K

K

K

E

E

N

N

I

I

D

D

F

F

V

V

Y

Y

Y

Y

G

G

G

G

Y
|
Y

Y

Y

P

P

E

E

M

M

G

G

Q

Q

M

M

L

L

R

R

Q

Q

A

A

R

R

S

S

V

V

G

G

L

L

K

K

T

T

Q

Q

F

F

M

M

G

G

P

P

E
|
E

G
|
G

V

V

G

G

N

N

A

A

S

S

L

L

S

S

N

N

I

I

A

A

G

G

D

D

A

A

A

A

E

E

G

G

M

M

L

L

V

V

T

T

M

M

P

P

K

K

R

R

Y

Y

D

D

Q

Q

D

D

P

P

A

A

N

N

Q

Q

G

G

I

I

V

V

D

D

A

A

L

L

K

K

A

A

D

D

K

K

K

K

D

D

P

P

S

S

G

G

P

P

Y
|
Y

V

V

W

W

I

I

T

T

Y

Y

A

A

A

A

V

V

Q

Q

S

S

L

L

A

A

T

T

A

A

L

L

E

E

R

R

T

T

G

G

S

S

D

D

E

E

P

P

L

L

A

A

L

L

V

V

K

K

D

D

L

L

K

K

A

A

N

N

G

G

A

A

N

N

T

T

V

V

I

I

G

G

P

P

L

L

N

N

W

W

D

D

E

E

K

K

G

G

D

D

L

L

K

K

G

G

F

F

D

D

F

F

G

G

V

V

F

F

Q

Q

W

W

H

H

A

A

D

D

G

G

S

S

S

S

T

T

A

K

A

A

binding leucine: W18 (= W41), L77 (= L100), C78 (= C101), S79 (= S102), G100 (= G123), A101 (= A124), T102 (= T125), Y150 (= Y173), Y202 (= Y225), E226 (= E249), G227 (= G250), Y276 (= Y299)

1usiA L-leucine-binding protein with phenylalanine bound (see paper)
100% identity, 93% coverage: 24:368/369 of query aligns to 1:345/345 of 1usiA

query
sites
1usiA

D

D

D

D

I

I

K

K

V

V

A

A

V

V

V

V

G

G

A

A

M

M

S

S

G

G

P

P

I

I

A

A

Q

Q

W
|
W

G

G

D

D

M

M

E

E

F

F

N

N

G

G

A

A

R

R

Q

Q

A

A

I

I

K

K

D

D

I

I

N

N

A

A

K

K

G

G

G

G

I

I

K

K

G

G

D

D

K

K

L

L

V

V

G

G

V

V

E

E

Y

Y

D

D

D

D

A

A

C

C

D

D

P

P

K

K

Q

Q

A

A

V

V

A

A

V

V

A

A

N

N

K

K

I

I

V

V

N

N

D

D

G

G

I

I

K

K

Y

Y

V

V

I

I

G

G

H

H

L
|
L

C
|
C

S
|
S

S

S

S

S

T

T

Q

Q

P

P

A

A

S

S

D

D

I

I

Y

Y

E

E

D

D

E

E

G

G

I

I

L

L

M

M

I

I

S

S

P

P

G
|
G

A
|
A

T
|
T

N

N

P

P

E

E

L

L

T

T

Q

Q

R

R

G

G

Y

Y

Q

Q

H

H

I

I

M

M

R

R

T

T

A

A

G

G

L

L

D

D

S

S

S

S

Q

Q

G

G

P

P

T

T

A

A

A

A

K

K

Y

Y

I

I

L

L

E

E

T

T

V

V

K

K

P

P

Q

Q

R

R

I

I

A

A

I

I

I

I

H

H

D

D

K

K

Q

Q

Q

Q

Y
|
Y

G

G

E

E

G

G

L

L

A

A

R

R

S

S

V

V

Q

Q

D

D

G

G

L

L

K

K

A

A

A

A

N

N

A

A

N

N

V

V

V

V

F

F

F

F

D

D

G

G

I

I

T

T

A

A

G

G

E

E

K

K

D

D

F

F

S

S

A

A

L

L

I

I

A

A

R

R

L

L

K

K

K

K

E

E

N

N

I

I

D

D

F

F

V

V

Y

Y

Y

Y

G

G

G

G

Y
|
Y

Y

Y

P

P

E

E

M

M

G

G

Q

Q

M

M

L

L

R

R

Q

Q

A

A

R

R

S

S

V

V

G

G

L

L

K

K

T

T

Q

Q

F

F

M

M

G

G

P

P

E
|
E

G
|
G

V

V

G

G

N

N

A

A

S

S

L

L

S

S

N

N

I

I

A

A

G

G

D

D

A

A

A

A

E

E

G

G

M

M

L

L

V

V

T

T

M

M

P

P

K

K

R

R

Y

Y

D

D

Q

Q

D

D

P

P

A

A

N

N

Q

Q

G

G

I

I

V

V

D

D

A

A

L

L

K

K

A

A

D

D

K

K

K

K

D

D

P

P

S

S

G

G

P

P

Y
|
Y

V

V

W

W

I

I

T

T

Y

Y

A

A

A

A

V

V

Q

Q

S

S

L

L

A

A

T

T

A

A

L

L

E

E

R

R

T

T

G

G

S

S

D

D

E

E

P

P

L

L

A

A

L

L

V

V

K

K

D

D

L

L

K

K

A

A

N

N

G

G

A

A

N

N

T

T

V

V

I

I

G

G

P

P

L

L

N

N

W

W

D

D

E

E

K

K

G

G

D

D

L

L

K

K

G

G

F

F

D

D

F

F

G

G

V

V

F

F

Q

Q

W

W

H

H

A

A

D

D

G

G

S

S

S

S

T

T

A

K

A

A

binding phenylalanine: W18 (= W41), L77 (= L100), C78 (= C101), S79 (= S102), G100 (= G123), A101 (= A124), T102 (= T125), Y150 (= Y173), Y202 (= Y225), E226 (= E249), G227 (= G250), Y276 (= Y299)

1z18A Crystal structure analysis of periplasmic leu/ile/val-binding protein with bound valine (see paper)
79% identity, 94% coverage: 24:369/369 of query aligns to 1:344/344 of 1z18A

query
sites
1z18A

D

E

D

D

I

I

K

K

V

V

A

A

V

V

V

V

G

G

A

A

M

M

S

S

G

G

P

P

I

V

A

A

Q

Q

W
x
Y

G

G

D

D

M

Q

E

E

F

F

N

T

G

G

A

A

R

E

Q

Q

A

A

I

V

K

A

D

D

I

I

N

N

A

A

K

K

G

G

G

G

I

I

K

K

G

G

D

N

K

K

L

L

V

Q

G

I

V

V

E

K

Y

Y

D

D

D

D

A

A

C

C

D

D

P

P

K

K

Q

Q

A

A

V

V

A

A

V

V

A

A

N

N

K

K

I

V

V

V

N

N

D

D

G

G

I

I

K

K

Y

Y

V

V

I

I

G

G

H

H

L
|
L

C
|
C

S
|
S

S

S

S

S

T

T

Q

Q

P

P

A

A

S

S

D

D

I

I

Y

Y

E

E

D

D

E

E

G

G

I

I

L

L

M

M

I

I

S

T

P

P

G
x
A

A
|
A

T
|
T

N

A

P

P

E

E

L

L

T

T

Q

A

R

R

G

G

Y

Y

Q

Q

H

L

I

I

M

L

R

R

T

T

A

T

G

G

L

L

D

D

S

S

S

D

Q

Q

G

G

P

P

T

T

A

A

A

A

K

K

Y

Y

I

I

L

L

E

E

T

K

V

V

K

K

P

P

Q

Q

R

R

I

I

A

A

I

I

I

V

H

H

D

D

K

K

Q

Q

Q

Q

Y
|
Y

G

G

E

E

G

G

L

L

A

A

R

R

S

A

V

V

Q

Q

D

D

G

G

L

L

K

K

A

K

A

G

N

N

A

A

N

N

V

V

V

V

F

F

F

F

D

D

G

G

I

I

T

T

A

A

G

G

E

E

K

K

D

D

F

F

S

S

A

T

L

L

I

V

A

A

R

R

L

L

K

K

K

K

E

E

N

N

I

I

D

D

F

F

V

V

Y

Y

Y

Y

G

G

G

G

Y
|
Y

Y

H

P

P

E

E

M

M

G

G

Q

Q

M

I

L

L

R

R

Q

Q

A

A

R

R

S

A

V

A

G

G

L

L

K

K

T

T

Q

Q

F

F

M

M

G

G

P

P

E
|
E

G

G

V

V

G

A

N

N

A

V

S

S

L

L

S

S

N

N

I

I

A

A

G

G

D

E

A

S

A

A

E

E

G

G

M

L

L

L

V

V

T

T

M

K

P

P

K

K

R

N

Y

Y

D

D

Q

Q

D

V

P

P

A

A

N

N

Q

K

G

P

I

I

V

V

D

D

A

A

L

I

K

K

A

A

D

K

K

K

K

Q

D

D

P

P

S

S

G

G

P

A

Y
x
F

V

V

W

W

I

T

T

T

Y

Y

A

A

A

A

V

L

Q

Q

S

S

L

L

A

Q

T

A

A

G

L

L

E

N

R

Q

T

-

G

-

S

S

D

D

E

D

P

P

L

A

A

E

L

I

V

A

K

K

D

Y

L

L

K

K

A

A

N

N

G

S

A

V

N

D

T

T

V

V

I

M

G

G

P

P

L

L

N

T

W

W

D

D

E

E

K

K

G

G

D

D

L

L

K

K

G

G

F

F

D

E

F

F

G

G

V

V

F

F

Q

D

W

W

H

H

A

A

D

N

G

G

S

T

S

A

T

T

A

D

A

A

K

K

binding valine: Y18 (≠ W41), L77 (= L100), C78 (= C101), S79 (= S102), A100 (≠ G123), A101 (= A124), T102 (= T125), Y150 (= Y173), Y202 (= Y225), E226 (= E249), F276 (≠ Y299)

1z17A Crystal structure analysis of periplasmic leu/ile/val-binding protein with bound ligand isoleucine (see paper)
79% identity, 94% coverage: 24:369/369 of query aligns to 1:344/344 of 1z17A

query
sites
1z17A

D

E

D

D

I

I

K

K

V

V

A

A

V

V

V

V

G

G

A

A

M

M

S

S

G

G

P

P

I

V

A

A

Q

Q

W
x
Y

G

G

D

D

M

Q

E

E

F

F

N

T

G

G

A

A

R

E

Q

Q

A

A

I

V

K

A

D

D

I

I

N

N

A

A

K

K

G

G

G

G

I

I

K

K

G

G

D

N

K

K

L

L

V

Q

G

I

V

V

E

K

Y

Y

D

D

D

D

A

A

C

C

D

D

P

P

K

K

Q

Q

A

A

V

V

A

A

V

V

A

A

N

N

K

K

I

V

V

V

N

N

D

D

G

G

I

I

K

K

Y

Y

V

V

I

I

G

G

H

H

L
|
L

C
|
C

S
|
S

S

S

S

S

T

T

Q

Q

P

P

A

A

S

S

D

D

I

I

Y

Y

E

E

D

D

E

E

G

G

I

I

L

L

M

M

I

I

S

T

P

P

G
x
A

A
|
A

T
|
T

N

A

P

P

E

E

L

L

T

T

Q

A

R

R

G

G

Y

Y

Q

Q

H

L

I

I

M

L

R

R

T

T

A

T

G

G

L

L

D

D

S

S

S

D

Q

Q

G

G

P

P

T

T

A

A

A

A

K

K

Y

Y

I

I

L

L

E

E

T

K

V

V

K

K

P

P

Q

Q

R

R

I

I

A

A

I

I

I

V

H

H

D

D

K

K

Q

Q

Q

Q

Y
|
Y

G

G

E

E

G

G

L

L

A

A

R

R

S

A

V

V

Q

Q

D

D

G

G

L

L

K

K

A

K

A

G

N

N

A

A

N

N

V

V

V

V

F

F

F

F

D

D

G

G

I

I

T

T

A

A

G

G

E

E

K

K

D

D

F

F

S

S

A

T

L

L

I

V

A

A

R

R

L

L

K

K

K

K

E

E

N

N

I

I

D

D

F

F

V

V

Y

Y

Y

Y

G

G

G

G

Y
|
Y

Y

H

P

P

E

E

M

M

G

G

Q

Q

M

I

L

L

R

R

Q

Q

A

A

R

R

S

A

V

A

G

G

L

L

K

K

T

T

Q

Q

F

F

M

M

G

G

P

P

E
|
E

G
|
G

V

V

G

A

N

N

A

V

S

S

L

L

S

S

N

N

I

I

A

A

G

G

D

E

A

S

A

A

E

E

G

G

M

L

L

L

V

V

T

T

M

K

P

P

K

K

R

N

Y

Y

D

D

Q

Q

D

V

P

P

A

A

N

N

Q

K

G

P

I

I

V

V

D

D

A

A

L

I

K

K

A

A

D

K

K

K

K

Q

D

D

P

P

S

S

G

G

P

A

Y
x
F

V

V

W

W

I

T

T

T

Y

Y

A

A

A

A

V

L

Q

Q

S

S

L

L

A

Q

T

A

A

G

L

L

E

N

R

Q

T

-

G

-

S

S

D

D

E

D

P

P

L

A

A

E

L

I

V

A

K

K

D

Y

L

L

K

K

A

A

N

N

G

S

A

V

N

D

T

T

V

V

I

M

G

G

P

P

L

L

N

T

W

W

D

D

E

E

K

K

G

G

D

D

L

L

K

K

G

G

F

F

D

E

F

F

G

G

V

V

F

F

Q

D

W

W

H

H

A

A

D

N

G

G

S

T

S

A

T

T

A

D

A

A

K

K

binding isoleucine: Y18 (≠ W41), L77 (= L100), C78 (= C101), S79 (= S102), A100 (≠ G123), A101 (= A124), T102 (= T125), Y150 (= Y173), Y202 (= Y225), E226 (= E249), G227 (= G250), F276 (≠ Y299)

1z16A Crystal structure analysis of periplasmic leu/ile/val-binding protein with bound leucine (see paper)
79% identity, 94% coverage: 24:369/369 of query aligns to 1:344/344 of 1z16A

query
sites
1z16A

D

E

D

D

I

I

K

K

V

V

A

A

V

V

V

V

G

G

A

A

M

M

S

S

G

G

P

P

I

V

A

A

Q

Q

W
x
Y

G

G

D

D

M

Q

E

E

F

F

N

T

G

G

A

A

R

E

Q

Q

A

A

I

V

K

A

D

D

I

I

N

N

A

A

K

K

G

G

G

G

I

I

K

K

G

G

D

N

K

K

L

L

V

Q

G

I

V

V

E

K

Y

Y

D

D

D

D

A

A

C

C

D

D

P

P

K

K

Q

Q

A

A

V

V

A

A

V

V

A

A

N

N

K

K

I

V

V

V

N

N

D

D

G

G

I

I

K

K

Y

Y

V

V

I

I

G

G

H

H

L
|
L

C
|
C

S
|
S

S

S

S

S

T

T

Q

Q

P

P

A

A

S

S

D

D

I

I

Y

Y

E

E

D

D

E

E

G

G

I

I

L

L

M

M

I

I

S

T

P

P

G
x
A

A
|
A

T
|
T

N

A

P

P

E

E

L

L

T

T

Q

A

R

R

G

G

Y

Y

Q

Q

H

L

I

I

M

L

R

R

T

T

A

T

G

G

L

L

D

D

S

S

S

D

Q

Q

G

G

P

P

T

T

A

A

A

A

K

K

Y

Y

I

I

L

L

E

E

T

K

V

V

K

K

P

P

Q

Q

R

R

I

I

A

A

I

I

I

V

H

H

D

D

K

K

Q

Q

Q

Q

Y
|
Y

G

G

E

E

G

G

L

L

A

A

R

R

S

A

V

V

Q

Q

D

D

G

G

L

L

K

K

A

K

A

G

N

N

A

A

N

N

V

V

V

V

F

F

F

F

D

D

G

G

I

I

T

T

A

A

G

G

E

E

K

K

D

D

F

F

S

S

A

T

L

L

I

V

A

A

R

R

L

L

K

K

K

K

E

E

N

N

I

I

D

D

F

F

V

V

Y

Y

Y

Y

G

G

G

G

Y
|
Y

Y

H

P

P

E

E

M

M

G

G

Q

Q

M

I

L

L

R

R

Q

Q

A

A

R

R

S

A

V

A

G

G

L

L

K

K

T

T

Q

Q

F

F

M

M

G

G

P

P

E
|
E

G
|
G

V

V

G

A

N

N

A

V

S

S

L

L

S

S

N

N

I

I

A

A

G

G

D

E

A

S

A

A

E

E

G

G

M

L

L

L

V

V

T

T

M

K

P

P

K

K

R

N

Y

Y

D

D

Q

Q

D

V

P

P

A

A

N

N

Q

K

G

P

I

I

V

V

D

D

A

A

L

I

K

K

A

A

D

K

K

K

K

Q

D

D

P

P

S

S

G

G

P

A

Y
x
F

V

V

W

W

I

T

T

T

Y

Y

A

A

A

A

V

L

Q

Q

S

S

L

L

A

Q

T

A

A

G

L

L

E

N

R

Q

T

-

G

-

S

S

D

D

E

D

P

P

L

A

A

E

L

I

V

A

K

K

D

Y

L

L

K

K

A

A

N

N

G

S

A

V

N

D

T

T

V

V

I

M

G

G

P

P

L

L

N

T

W

W

D

D

E

E

K

K

G

G

D

D

L

L

K

K

G

G

F

F

D

E

F

F

G

G

V

V

F

F

Q

D

W

W

H

H

A

A

D

N

G

G

S

T

S

A

T

T

A

D

A

A

K

K

binding leucine: Y18 (≠ W41), L77 (= L100), C78 (= C101), S79 (= S102), A100 (≠ G123), A101 (= A124), T102 (= T125), Y150 (= Y173), Y202 (= Y225), E226 (= E249), G227 (= G250), F276 (≠ Y299)

4n0qB Crystal structure of an abc transporter, substrate-binding protein from brucella melitensis 16m in complex with l-leucine using a crystal grown in a crystal former (microlytic)
40% identity, 91% coverage: 25:359/369 of query aligns to 2:337/345 of 4n0qB

query
sites
4n0qB

D

D

I

I

K

T

V

I

A

G

V

V

V

I

G

A

A

P

M

L

S

T

G

G

P

P

I

V

A

A

Q

A

W
x
F

G

G

D

D

M

Q

E

V

F

K

N

K

G

G

A

A

R

E

Q

T

A

A

I

V

K

E

D

V

I

I

N

N

A

K

K

A

G

G

G

G

I

I

K

K

G

G

D

E

K

K

L

V

V

V

G

L

V

K

E

F

Y

A

D

D

D

D

A

A

C

G

D

E

P

P

K

K

Q

Q

A

G

V

V

A

S

V

A

A

A

N

N

K

Q

I

I

V

V

N

G

D

D

G

G

I

I

K

K

Y

F

V

V

I

V

G

G

H

L

L
x
V

C
x
T

S
x
T

S

G

S

V

T

A

Q

V

P

P

A

V

S

S

D

D

I

V

Y

L

E

S

D

E

E

N

G

G

I

V

L

L

M

M

I

V

S

T

P

P

G
x
T

A
|
A

T
|
T

N

G

P

P

E

D

L

L

T

T

Q

A

R

R

G

G

Y

L

Q

E

H

N

I

V

M

F

R

R

T

T

A

C

G

G

L

R

D

D

S

G

S

Q

Q

Q

G

A

P

E

T

V

A

M

A

A

K

D

Y

Y

I

V

L

L

E

K

T

N

V

M

K

K

P

D

Q

K

R

K

I

V

A

A

I

V

I

I

H

H

D

D

K

K

Q

G

Q

A

Y
|
Y

G

G

E

K

G

G

L

L

A

A

R

D

S

A

V

F

Q

K

D

A

G

A

L

I

K

N

A

K

A

G

N

G

A

I

N

T

V

E

V

V

F

H

F

Y

D

D

G

S

I

V

T

T

A

P

G

G

E

D

K

K

D

D

F

F

S

S

A

A

L

L

I

V

A

T

R

K

L

L

K

K

K

S

E

A

N

G

I

A

D

E

F

V

V

V

Y

Y

Y

F

G

G

G

G

Y
|
Y

Y

H

P

A

E

E

M

G

G

G

Q

L

M

L

L

S

R

R

Q

Q

A

L

R

H

S

D

V

A

G

G

L

M

K

Q

T

A

Q

L

F

V

M

L

G

G

P

G

E
|
E

G
|
G

V

L

G

S

N

N

A

T

S

E

L

Y

S

W

N

A

I

I

A

G

G

G

D

T

A

N

A

A

E

Q

G

G

M

T

L

L

V

F

T

T

M

N

P

A

K

K

R

D

Y

A

D

T

Q

K

D

N

P

P

A

A

N

A

Q

K

G

D

I

A

V

I

D

Q

A

A

L

L

K

K

A

A

D

-

K

K

K

N

D

I

P

P

S

A

G

E

P

A

Y
x
F

V

T

W

M

I

N

T

A

Y

Y

A

A

A

A

V

V

Q

E

S

V

L

I

A

K

T

A

A

G

L

I

E

E

R

R

T

A

G

G

S

S

-

T

D

D

E

D

P

S

L

A

A

A

L

V

V

A

K

K

D

A

L

L

K

H

-

D

A

G

N

K

G

P

A

I

N

E

T

T

V

A

I

I

G

G

P

T

L

L

N

T

W

Y

D

S

E

E

K

T

G

G

D

D

L

L

K

S

G

S

F

P

D

S

F

F

G

D

V

I

F

F

Q

K

W

W

binding leucine: F18 (≠ W41), V77 (≠ L100), T78 (≠ C101), T79 (≠ S102), T100 (≠ G123), A101 (= A124), T102 (= T125), Y150 (= Y173), Y202 (= Y225), E226 (= E249), G227 (= G250), F275 (≠ Y299)

3ip9A Structure of atu2422-gaba receptor in complex with gaba (see paper)
41% identity, 91% coverage: 25:359/369 of query aligns to 2:335/348 of 3ip9A

query
sites
3ip9A

D

D

I

V

K

V

V

I

A

A

V

V

V

G

G

A

A

P

M

L

S

T

G

G

P

P

I

N

A

A

Q

A

W

F

G

G

D

A

M

Q

E

I

F

Q

N

K

G

G

A

A

R

E

Q

Q

A

A

I

A

K

K

D

D

I

I

N

N

A

A

K

A

G

G

G

G

I

I

K

N

G

G

D

E

K

Q

L

I

V

K

G

I

V

V

E

L

Y

G

D

D

D

D

A

V

C

S

D

D

P

P

K

K

Q

Q

A

G

V

I

A

S

V

V

A

A

N

N

K

K

I

F

V

V

N

A

D

D

G

G

I

V

K

K

Y

F

V

V

I

V

G

G

H

H

L
x
F

C
x
N

S
|
S

S

G

S

V

T

S

Q

I

P

P

A

A

S

S

D

E

I

V

Y

Y

E

A

D

E

E

N

G

G

I

I

L

L

M

E

I

I

S

T

P

P

G
x
A

A
|
A

T
|
T

N

N

P

P

E

V

L

F

T

T

Q

E

R

R

G

G

Y

L

Q

W

H

N

I

T

M

F

R

R

T

T

A

C

G

G

L

R

D

D

S

D

S

Q

Q

Q

G

G

P

G

T

I

A

A

A

G

K

K

Y

Y

I

L

L

A

E

D

T

H

V

F

K

K

P

D

Q

A

R

K

I

V

A

A

I

I

I

I

H

H

D

D

K

K

Q

T

Q

P

Y
|
Y

G

G

E

Q

G

G

L

L

A

A

R

D

S

E

V

T

Q

K

D

K

G

A

L

A

K

N

A

A

A

A

N

G

A

V

N

T

V

E

V

V

F

M

F

Y

D

E

G

G

I

V

T

N

A

V

G

G

E

D

K

K

D

D

F

F

S

S

A

A

L

L

I

I

A

S

R

K

L

M

K

K

K

E

E

A

N

G

I

V

D

S

F

I

V

I

Y

Y

Y

W

G

G

G

G

Y

L

Y

H

P

T

E

E

M

A

G

G

Q

L

M

I

L

I

R

R

Q

Q

A

A

R

A

S

D

V

Q

G

G

L

L

K

K

T

A

Q

K

F

L

M

V

G

S

P

G

E
x
D

G
|
G

V

I

G

V

N

S

A

N

S

E

L

L

S

A

N

S

I

I

A

A

G

G

D

D

A

A

A

V

E

E

G

G

M

T

L

L

V

N

T

T

M

F

P

G

K

P

R

D

Y

P

D

T

Q

L

D

R

P

P

A

E

N

N

Q

K

G

E

I

L

V

V

D

E

A

K

L

F

K

K

A

A

D

A

K

G

K

F

D

N

P

P

S

E

G

A

P

-

Y
|
Y

V

T

W

L

I

Y

T

S

Y

Y

A

A

A

A

V

M

Q

Q

S

A

L

I

A

A

T

G

A

A

L

A

E

K

R

A

T

A

G

G

S

S

D

V

E

E

P

P

L

E

A

K

L

V

V

A

K

E

D

A

L

L

K

K

A

K

N

G

G

S

A

F

N

P

T

T

V

A

I

L

G

G

P

E

L

I

N

S

W

F

D

D

E

E

K

K

G

G

D

D

L

P

K

K

G

L

F

P

D

G

F

Y

G

V

V

M

F

Y

Q

E

W

W

binding gamma-amino-butanoic acid: F77 (≠ L100), N78 (≠ C101), S79 (= S102), A100 (≠ G123), A101 (= A124), T102 (= T125), Y150 (= Y173), D226 (≠ E249), G227 (= G250), Y275 (= Y299)

3ip7A Structure of atu2422-gaba receptor in complex with valine (see paper)
41% identity, 91% coverage: 25:359/369 of query aligns to 2:335/348 of 3ip7A

query
sites
3ip7A

D

D

I

V

K

V

V

I

A

A

V

V

V

G

G

A

A

P

M

L

S

T

G

G

P

P

I

N

A

A

Q

A

W

F

G

G

D

A

M

Q

E

I

F

Q

N

K

G

G

A

A

R

E

Q

Q

A

A

I

A

K

K

D

D

I

I

N

N

A

A

K

A

G

G

G

G

I

I

K

N

G

G

D

E

K

Q

L

I

V

K

G

I

V

V

E

L

Y

G

D

D

D

D

A

V

C

S

D

D

P

P

K

K

Q

Q

A

G

V

I

A

S

V

V

A

A

N

N

K

K

I

F

V

V

N

A

D

D

G

G

I

V

K

K

Y

F

V

V

I

V

G

G

H

H

L
x
F

C
x
N

S
|
S

S
x
G

S

V

T

S

Q

I

P

P

A

A

S

S

D

E

I

V

Y

Y

E

A

D

E

E

N

G

G

I

I

L

L

M

E

I

I

S

T

P

P

G
x
A

A
|
A

T
|
T

N

N

P

P

E

V

L

F

T

T

Q

E

R

R

G

G

Y

L

Q

W

H

N

I

T

M

F

R

R

T

T

A

C

G

G

L

R

D

D

S

D

S

Q

Q

Q

G

G

P

G

T

I

A

A

A

G

K

K

Y

Y

I

L

L

A

E

D

T

H

V

F

K

K

P

D

Q

A

R

K

I

V

A

A

I

I

I

I

H

H

D

D

K

K

Q
x
T

Q
x
P

Y
|
Y

G

G

E

Q

G

G

L

L

A

A

R

D

S

E

V

T

Q

K

D

K

G

A

L

A

K

N

A

A

A

A

N

G

A

V

N

T

V

E

V

V

F

M

F

Y

D

E

G

G

I

V

T

N

A

V

G

G

E

D

K

K

D

D

F

F

S

S

A

A

L

L

I

I

A

S

R

K

L

M

K

K

K

E

E

A

N

G

I

V

D

S

F

I

V

I

Y

Y

Y

W

G

G

G

G

Y
x
L

Y

H

P

T

E

E

M

A

G

G

Q

L

M

I

L

I

R

R

Q

Q

A

A

R

A

S

D

V

Q

G

G

L

L

K

K

T

A

Q

K

F

L

M

V

G

S

P

G

E
x
D

G
|
G

V

I

G

V

N

S

A

N

S

E

L

L

S

A

N

S

I

I

A

A

G

G

D

D

A

A

A

V

E

E

G

G

M

T

L

L

V

N

T

T

M

F

P

G

K

P

R

D

Y

P

D

T

Q

L

D

R

P

P

A

E

N

N

Q

K

G

E

I

L

V

V

D

E

A

K

L

F

K

K

A

A

D

A

K

G

K

F

D

N

P

P

S

E

G

A

P

-

Y

Y

V

T

W

L

I

Y

T

S

Y

Y

A

A

A

A

V

M

Q

Q

S

A

L

I

A

A

T

G

A

A

L

A

E

K

R

A

T

A

G

G

S

S

D

V

E

E

P

P

L

E

A

K

L

V

V

A

K

E

D

A

L

L

K
|
K

A

K

N

G

G

S

A

F

N

P

T

T

V

A

I

L

G

G

P

E

L

I

N

S

W
x
F

D

D

E

E

K

K

G

G

D

D

L

P

K
|
K

G

L

F

P

D

G

F

Y

G

V

V

M

F

Y

Q

E

W

W

binding calcium ion: S79 (= S102), G80 (≠ S103), T148 (≠ Q171), P149 (≠ Q172), Y150 (= Y173), K306 (= K330), F319 (≠ W343), K326 (= K350)
binding valine: F77 (≠ L100), N78 (≠ C101), S79 (= S102), A100 (≠ G123), A101 (= A124), T102 (= T125), Y150 (= Y173), L202 (≠ Y225), D226 (≠ E249), G227 (= G250)

3ip6A Structure of atu2422-gaba receptor in complex with proline (see paper)
41% identity, 91% coverage: 25:359/369 of query aligns to 2:335/348 of 3ip6A

query
sites
3ip6A

D

D

I

V

K

V

V

I

A

A

V

V

V

G

G

A

A

P

M

L

S

T

G

G

P

P

I

N

A

A

Q

A

W

F

G

G

D

A

M

Q

E

I

F

Q

N

K

G

G

A

A

R

E

Q

Q

A

A

I

A

K

K

D

D

I

I

N

N

A

A

K

A

G

G

G

G

I

I

K

N

G

G

D

E

K

Q

L

I

V

K

G

I

V

V

E

L

Y

G

D

D

D

D

A

V

C

S

D

D

P

P

K

K

Q

Q

A

G

V

I

A

S

V

V

A

A

N

N

K

K

I

F

V

V

N

A

D

D

G

G

I

V

K

K

Y

F

V

V

I

V

G

G

H

H

L
x
F

C
x
N

S
|
S

S

G

S

V

T

S

Q

I

P

P

A

A

S

S

D

E

I

V

Y

Y

E

A

D

E

E

N

G

G

I

I

L

L

M

E

I

I

S

T

P

P

G
x
A

A
|
A

T
|
T

N

N

P

P

E

V

L

F

T

T

Q

E

R

R

G

G

Y

L

Q

W

H

N

I

T

M

F

R

R

T

T

A

C

G

G

L

R

D

D

S

D

S

Q

Q

Q

G

G

P

G

T

I

A

A

A

G

K

K

Y

Y

I

L

L

A

E

D

T

H

V

F

K

K

P

D

Q

A

R

K

I

V

A

A

I

I

I

I

H

H

D

D

K

K

Q

T

Q

P

Y
|
Y

G

G

E

Q

G

G

L

L

A

A

R

D

S

E

V

T

Q

K

D

K

G

A

L

A

K

N

A

A

A

A

N

G

A

V

N

T

V

E

V

V

F

M

F

Y

D

E

G

G

I

V

T

N

A

V

G

G

E

D

K

K

D

D

F

F

S

S

A

A

L

L

I

I

A

S

R

K

L

M

K

K

K

E

E

A

N

G

I

V

D

S

F

I

V

I

Y

Y

Y

W

G

G

G

G

Y

L

Y

H

P

T

E

E

M

A

G

G

Q

L

M

I

L

I

R

R

Q

Q

A

A

R

A

S

D

V

Q

G

G

L

L

K

K

T

A

Q

K

F

L

M

V

G

S

P

G

E
x
D

G
|
G

V

I

G

V

N

S

A

N

S

E

L

L

S

A

N

S

I

I

A

A

G

G

D

D

A

A

A

V

E

E

G

G

M

T

L

L

V

N

T

T

M

F

P

G

K

P

R

D

Y

P

D

T

Q

L

D

R

P

P

A

E

N

N

Q

K

G

E

I

L

V

V

D

E

A

K

L

F

K

K

A

A

D

A

K

G

K

F

D

N

P

P

S

E

G

A

P

-

Y
|
Y

V

T

W

L

I

Y

T

S

Y

Y

A

A

A

A

V

M

Q

Q

S

A

L

I

A

A

T

G

A

A

L

A

E

K

R

A

T

A

G

G

S

S

D

V

E

E

P

P

L

E

A

K

L

V

V

A

K

E

D

A

L

L

K

K

A

K

N

G

G

S

A

F

N

P

T

T

V

A

I

L

G

G

P

E

L

I

N

S

W

F

D

D

E

E

K

K

G

G

D

D

L

P

K

K

G

L

F

P

D

G

F

Y

G

V

V

M

F

Y

Q

E

W

W

binding proline: F77 (≠ L100), N78 (≠ C101), S79 (= S102), A100 (≠ G123), A101 (= A124), T102 (= T125), Y150 (= Y173), D226 (≠ E249), G227 (= G250), Y275 (= Y299)

3ip5A Structure of atu2422-gaba receptor in complex with alanine (see paper)
41% identity, 91% coverage: 25:359/369 of query aligns to 2:335/348 of 3ip5A

query
sites
3ip5A

D

D

I

V

K

V

V

I

A

A

V

V

V

G

G

A

A

P

M

L

S

T

G

G

P

P

I

N

A

A

Q

A

W

F

G

G

D

A

M

Q

E

I

F

Q

N

K

G

G

A

A

R

E

Q

Q

A

A

I

A

K

K

D

D

I

I

N

N

A

A

K

A

G

G

G

G

I

I

K

N

G

G

D

E

K

Q

L

I

V

K

G

I

V

V

E

L

Y

G

D

D

D

D

A

V

C

S

D

D

P

P

K

K

Q

Q

A

G

V

I

A

S

V

V

A

A

N

N

K

K

I

F

V

V

N

A

D

D

G

G

I

V

K

K

Y

F

V

V

I

V

G

G

H

H

L
x
F

C
x
N

S
|
S

S

G

S

V

T

S

Q

I

P

P

A

A

S

S

D

E

I

V

Y

Y

E

A

D

E

E

N

G

G

I

I

L

L

M

E

I

I

S

T

P

P

G
x
A

A
|
A

T
|
T

N

N

P

P

E

V

L

F

T

T

Q

E

R

R

G

G

Y

L

Q

W

H

N

I

T

M

F

R

R

T

T

A

C

G

G

L

R

D

D

S

D

S

Q

Q

Q

G

G

P

G

T

I

A

A

A

G

K

K

Y

Y

I

L

L

A

E

D

T

H

V

F

K

K

P

D

Q

A

R

K

I

V

A

A

I

I

I

I

H

H

D

D

K

K

Q

T

Q

P

Y
|
Y

G

G

E

Q

G

G

L

L

A

A

R

D

S

E

V

T

Q

K

D

K

G

A

L

A

K

N

A

A

A

A

N

G

A

V

N

T

V

E

V

V

F

M

F

Y

D

E

G

G

I

V

T

N

A

V

G

G

E

D

K

K

D

D

F

F

S

S

A

A

L

L

I

I

A

S

R

K

L

M

K

K

K

E

E

A

N

G

I

V

D

S

F

I

V

I

Y

Y

Y

W

G

G

G

G

Y

L

Y

H

P

T

E

E

M

A

G

G

Q

L

M

I

L

I

R

R

Q

Q

A

A

R

A

S

D

V

Q

G

G

L

L

K

K

T

A

Q

K

F

L

M

V

G

S

P

G

E
x
D

G

G

V

I

G

V

N

S

A

N

S

E

L

L

S

A

N

S

I

I

A

A

G

G

D

D

A

A

A

V

E

E

G

G

M

T

L

L

V

N

T

T

M

F

P

G

K

P

R

D

Y

P

D

T

Q

L

D

R

P

P

A

E

N

N

Q

K

G

E

I

L

V

V

D

E

A

K

L

F

K

K

A

A

D

A

K

G

K

F

D

N

P

P

S

E

G

A

P

-

Y

Y

V

T

W

L

I

Y

T

S

Y

Y

A

A

A

A

V

M

Q

Q

S

A

L

I

A

A

T

G

A

A

L

A

E

K

R

A

T

A

G

G

S

S

D

V

E

E

P

P

L

E

A

K

L

V

V

A

K

E

D

A

L

L

K

K

A

K

N

G

G

S

A

F

N

P

T

T

V

A

I

L

G

G

P

E

L

I

N

S

W

F

D

D

E

E

K

K

G

G

D

D

L

P

K

K

G

L

F

P

D

G

F

Y

G

V

V

M

F

Y

Q

E

W

W

binding alanine: F77 (≠ L100), N78 (≠ C101), S79 (= S102), A100 (≠ G123), A101 (= A124), T102 (= T125), Y150 (= Y173), D226 (≠ E249)

3ipcA Structure of atu2422-gaba f77a mutant receptor in complex with leucine (see paper)
41% identity, 91% coverage: 25:359/369 of query aligns to 2:335/348 of 3ipcA

query
sites
3ipcA

D

D

I

V

K

V

V

I

A

A

V

V

V

G

G

A

A

P

M

L

S

T

G

G

P

P

I

N

A

A

Q

A

W
x
F

G

G

D

A

M

Q

E

I

F

Q

N

K

G

G

A

A

R

E

Q

Q

A

A

I

A

K

K

D

D

I

I

N

N

A

A

K

A

G

G

G

G

I

I

K

N

G

G

D

E

K

Q

L

I

V

K

G

I

V

V

E

L

Y

G

D

D

D

D

A

V

C

S

D

D

P

P

K

K

Q

Q

A

G

V

I

A

S

V

V

A

A

N

N

K

K

I

F

V

V

N

A

D

D

G

G

I

V

K

K

Y

F

V

V

I

V

G

G

H

H

L
x
A

C
x
N

S
|
S

S

G

S

V

T

S

Q

I

P

P

A

A

S

S

D

E

I

V

Y

Y

E

A

D

E

E

N

G

G

I

I

L

L

M

E

I

I

S

T

P

P

G
x
A

A
|
A

T
|
T

N

N

P

P

E

V

L

F

T

T

Q

E

R

R

G

G

Y

L

Q

W

H

N

I

T

M

F

R

R

T

T

A

C

G

G

L

R

D

D

S

D

S

Q

Q

Q

G

G

P

G

T

I

A

A

A

G

K

K

Y

Y

I

L

L

A

E

D

T

H

V

F

K

K

P

D

Q

A

R

K

I

V

A

A

I

I

I

I

H

H

D

D

K

K

Q

T

Q

P

Y
|
Y

G

G

E

Q

G

G

L

L

A

A

R

D

S

E

V

T

Q

K

D

K

G

A

L

A

K

N

A

A

A

A

N

G

A

V

N

T

V

E

V

V

F

M

F

Y

D

E

G

G

I

V

T

N

A

V

G

G

E

D

K

K

D

D

F

F

S

S

A

A

L

L

I

I

A

S

R

K

L

M

K

K

K

E

E

A

N

G

I

V

D

S

F

I

V

I

Y

Y

Y

W

G

G

G

G

Y

L

Y

H

P

T

E

E

M

A

G

G

Q

L

M

I

L

I

R

R

Q

Q

A

A

R

A

S

D

V

Q

G

G

L

L

K

K

T

A

Q

K

F

L

M

V

G

S

P

G

E
x
D

G
|
G

V

I

G

V

N

S

A

N

S

E

L

L

S

A

N

S

I

I

A

A

G

G

D

D

A

A

A

V

E

E

G

G

M

T

L

L

V

N

T

T

M

F

P

G

K

P

R

D

Y

P

D

T

Q

L

D

R

P

P

A

E

N

N

Q

K

G

E

I

L

V

V

D

E

A

K

L

F

K

K

A

A

D

A

K

G

K

F

D

N

P

P

S

E

G

A

P

-

Y
|
Y

V

T

W

L

I

Y

T

S

Y

Y

A

A

A

A

V

M

Q

Q

S

A

L

I

A

A

T

G

A

A

L

A

E

K

R

A

T

A

G

G

S

S

D

V

E

E

P

P

L

E

A

K

L

V

V

A

K

E

D

A

L

L

K

K

A

K

N

G

G

S

A

F

N

P

T

T

V

A

I

L

G

G

P

E

L

I

N

S

W

F

D

D

E

E

K

K

G

G

D

D

L

P

K

K

G

L

F

P

D

G

F

Y

G

V

V

M

F

Y

Q

E

W

W

binding leucine: F18 (≠ W41), A77 (≠ L100), N78 (≠ C101), S79 (= S102), A100 (≠ G123), A101 (= A124), T102 (= T125), Y150 (= Y173), D226 (≠ E249), G227 (= G250), Y275 (= Y299)

4mlcA Abc transporter substrate-binding protein fromdesulfitobacterium hafniense
32% identity, 85% coverage: 37:348/369 of query aligns to 10:317/336 of 4mlcA

query
sites
4mlcA

P

P

I

F

A

A

Q

S

W

L

G

D

D

D

M

L

E

F

F

A

N

E

G

G

A

L

R

E

Q

L

A

A

I

V

K

Q

D

E

I

I

N

N

A

E

K

Q

G

G

G

G

I

V

K

Q

G

G

D

R

K

K

L

L

V

S

G

L

V

V

E

K

Y

A

D

D

D

D

A

E

C

A

D

E

P

L

K

E

Q

K

A

G

V

L

A

A

V

I

A

A

N

Q

K

A

I

F

V

A

-

D

N

N

D

A

G

G

I

I

K

Q

Y

A

V

V

I

I

G

G

H

H

L

R

C

N

S

S

S

F

S

I

T

S

Q

I

P

P

A

A

S

A

D

S

I

I

Y

Y

E

D

D

Q

E

A

G

G

I

L

L

V

M

M

I

L

S

S

P

P

G

A

A

S

T

T

N

S

P

P

E

D

L

L

T

T

Q

D

R

H

G

G

Y

Y

Q

I

H

H

I

V

M

F

R

R

T

N

A

I

G

P

L

S

D

D

S

Q

S

E

Q

I

G

A

P

R

T

Q

A

L

A

A

K

I

Y

Y

I

L

L

A

E

E

T

Q

V

-

K

G

P

H

Q
x
E

R

R

I

M

A

V

I

I

I

Y

H

Y

D

T

K

D

Q

D

Q

S

Y

Y

G

G

E

N

G

G

L

L

A

A

R

N

S

A

V

F

Q

E

D

D

G

Y

L

A

K

R

A

A

A

Q

N
x
G

A

I

N

T

V

I

V

V

F

D

F

R

D

F

G

N

I

Y

T

Y

A

G

G

N

E

L

K

K

D

D

F

L

S

E

A

R

L

L

I

Y

A

D

R

K

L

W

K

Q

K

A

E

F

N

G

I

M

D

D

F

G

V

I

Y

F

Y

I

G

A

G

K

Y

T

Y

A

P

T

E

G

M

G

G

G

-

T

Q

E

M

F

L

L

R

V

Q

D

A

A

R

K

S

S

V

V

G

G

L

I

K

E

T

V

Q

-

F

-

M

-

G

-

P

P

E

L

G

I

V

A

G

G

N

N

A

-

S

-

L

-

S

S

N

W

I

D

A

A

G

G

D

M

A

T

A

A

E

E

G

G

M

L

L

L

V

V

-

G

-

S

-

F

T

F

M

N

P

P

K

Q

R

R

Y

-

D

-

Q

P

D

D

P

S

A

R

N

T

Q

Q

G

D

I

F

V

V

D

E

A

A

L

F

K

R

A

R

D

E

K

Y

K

G

D

Q

P

P

S

P

G

T

P

S

Y

Y

V

A

W

A

I

A

T

G

Y

Y

A

D

A

A

V

V

Q

I

S

L

L

L

A

A

T

E

A

A

L

L

E

E

R

K

T

S

G

D

S

L

D

T

E

H

P

P

L

A

A

T

L

L

V

A

K

Q

D

G

L

L

K

R

A

D

N

L

G

G

A

P

-

W

N

E

T

G

V

V

I

M

G

G

P

M

L

H

N

R

W

F

D

D

E

G

K

R

G

G

D

D

binding calcium ion: E135 (≠ Q162), G162 (≠ N189)

4q6bA Crystal structure of abc transporter substrate-binding protein fromdesulfitobacterium hafniense complex with leu
31% identity, 85% coverage: 37:348/369 of query aligns to 10:316/335 of 4q6bA

query
sites
4q6bA

P

P

I

F

A

A

Q

S

W

L

G

D

D

D

M

L

E

F

F

A

N

E

G

G

A

L

R

E

Q

L

A

A

I

V

K

Q

D

E

I

I

N

N

A

E

K

Q

G

G

G

G

I

V

K

Q

G

G

D

R

K

K

L

L

V

S

G

L

V

V

E

K

Y

A

D

D

D

D

A

E

C

A

D

E

P

L

K

E

Q

K

A

G

V

L

A

A

V

I

A

A

N

Q

K

A

I

F

V

A

-

D

N

N

D

A

G

G

I

I

K

Q

Y

A

V

V

I

I

G

G

H

H

L
x
R

C
x
N

S
|
S

S

F

S

I

T

S

Q

I

P

P

A

A

S

A

D

S

I

I

Y

Y

E

D

D

Q

E

A

G

G

I

L

L

V

M

M

I

L

S

S

P

P

G
x
A

A
x
S

T
|
T

N

S

P

P

E

D

L

L

T

T

Q

D

R

H

G

G

Y

Y

Q

I

H

H

I

V

M

F

R

R

T

N

A

I

G

P

L

S

D

D

S

Q

S

E

Q

I

G

A

P

R

T

Q

A

L

A

A

K

I

Y

Y

I

L

L

A

E

E

T

Q

V

-

K

G

P

H

Q

E

R

R

I

M

A

V

I

I

I

Y

H

Y

D

T

K

D

Q

D

Q

S

Y

Y

G

G

E

N

G

G

L

L

A

A

R

N

S

A

V

F

Q

E

D

D

G

Y

L

A

K

R

A

A

A

Q

N

G

A

I

N

T

V

I

V

V

F
x
D

F
x
R

D

F

G

N

I

Y

T

Y

A

G

G

N

E

L

K

K

D

D

F

L

S

E

A

R

L

L

I

Y

A

D

R

K

L

W

K

Q

K

A

E

F

N

G

I

M

D

D

F

G

V

I

Y

F

Y

I

G

A

G

K

Y

T

Y

A

P

T

E

G

M

G

G

G

-

T

Q

E

M

F

L

L

R

V

Q

D

A

A

R

K

S

S

V

V

G

G

L

I

K

E

T

V

Q

-

F

-

M

-

G

-

P

P

E

L

G

I

V

A

G

G

N
|
N

A

S

S

W

L

D

S

A

N

M

I

-

A

-

G

-

D

-

A

T

A

A

E

E

G

G

M

L

L

L

V

V

-

G

-

S

-

F

T

F

M

N

P

P

K

Q

R

R

Y

-

D

-

Q

P

D

D

P

S

A

R

N

T

Q

Q

G

D

I

F

V

V

D

E

A

A

L

F

K

R

A

R

D

E

K

Y

K

G

D

Q

P

P

S

P

G

T

P

S

Y
|
Y

V

A

W

A

I

A

T

G

Y

Y

A

D

A

A

V

V

Q

I

S

L

L

L

A

A

T

E

A

A

L

L

E

E

R

K

T

S

G

D

S

L

D

T

E

H

P

P

L

A

A

T

L

L

V

A

K

Q

D

G

L

L

K

R

A

D

N

L

G

G

A

P

-

W

N

E

T

G

V

V

I

M

G

G

P

M

L

H

N

R

W

F

D

D

E

G

K

R

G

G

D

D

binding calcium ion: D167 (≠ F194), R168 (≠ F195)
binding leucine: R74 (≠ L100), N75 (≠ C101), S76 (= S102), A97 (≠ G123), S98 (≠ A124), T99 (= T125), N223 (= N253), Y266 (= Y299)

3td9A Crystal structure of a leucine binding protein livk (tm1135) from thermotoga maritima msb8 at 1.90 a resolution
28% identity, 88% coverage: 26:348/369 of query aligns to 2:324/350 of 3td9A

query
sites
3td9A

I

V

K

K

V

I

A

A

V

V

V

I

G

L

A

P

M

M

S

T

G

G

P

G

I

I

A

S

Q

A

W
x
F

G

G

D

R

M

M

E

V

F

W

N

E

G

G

A

I

R

Q

Q

I

A

A

I

H

K

E

D

E

I

-

N

-

A

-

K

K

G

P

G

T

I

V

K

L

G

G

D

E

K

E

L

V

V

E

G

L

V

V

E

L

Y

L

D

D

D

T

A

R

C

S

D

E

P

K

K

T

Q

E

A

A

V

A

A

N

V

A

A

A

N

A

K

R

I

A

V

I

N

D

-

K

D

E

G

K

I

V

K

L

Y

A

V

I

I

I

G

G

H

E

L
x
V

C
x
A

S
|
S

S

A

S

H

T

S

Q

L

P

A

A

I

S

A

D

P

I

I

Y

A

E

E

D

E

E

N

G

K

I

V

L

P

M

M

I

V

S

T

P

P

G
x
A

A
x
S

T
|
T

N

N

P

P

E

L

L

V

T

T

Q

Q

R

-

G

G

Y

R

Q

K

H

F

I

V

M

S

R

R

T

V

A

C

G

F

L

I

D

D

S

P

S

F

Q

Q

G

G

P

A

T

A

A

M

A

A

K

V

Y