SitesBLAST

A0QUX7 Acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS; EC 2.2.1.6 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
31% identity, 98% coverage: 1:94/96 of query aligns to 1:94/170 of A0QUX7

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A0QUX7

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K46 (≠ S47) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

P9WKJ3 Putative acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS; EC 2.2.1.6 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
35% identity, 81% coverage: 1:78/96 of query aligns to 1:76/168 of P9WKJ3

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P9WKJ3

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K44 (≠ S47) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

2pc6A Crystal structure of putative acetolactate synthase- small subunit from nitrosomonas europaea (see paper)
36% identity, 72% coverage: 10:78/96 of query aligns to 5:74/164 of 2pc6A

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2pc6A

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binding calcium ion: N66 (≠ D70), L68 (= L72), V71 (= V75)

Q9FFF4 Acetolactate synthase small subunit 2, chloroplastic; ALS-interacting protein 3; Acetohydroxy-acid synthase small subunit 2; Protein valine-tolerant 1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
27% identity, 78% coverage: 11:85/96 of query aligns to 79:152/477 of Q9FFF4

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Q9FFF4

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G88 (= G20) mutation to D: Resistance to valine ihibition.
T119 (≠ I52) mutation to I: In vat1-1; resistance to valine ihibition.

Sites not aligning to the query:

453 R→Q: In vat1-3; resistance to valine ihibition.

6vz8F Arabidopsis thaliana acetohydroxyacid synthase complex with valine bound (see paper)
35% identity, 74% coverage: 15:85/96 of query aligns to 11:80/159 of 6vz8F

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6vz8F

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binding valine: D13 (≠ N17), E14 (≠ H18), G16 (= G20), M17 (≠ V21), I18 (≠ M22), N31 (= N35), I32 (≠ V36)

Q93YZ7 Acetolactate synthase small subunit 1, chloroplastic; ALS-interacting protein 1; Acetohydroxyacid synthase small subunit 1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
32% identity, 78% coverage: 11:85/96 of query aligns to 89:162/491 of Q93YZ7

query
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Q93YZ7

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D95 (≠ N17) binding
G98 (= G20) mutation to E: Increased feedback inhibition by valine/isoleucine.
M99 (≠ V21) binding
I100 (≠ M22) binding
F107 (= F29) mutation to A: Decreased feedback inhibition by leucine and valine/isoleucine.
Y112 (≠ F34) mutation to A: Increased feedback inhibition by leucine.
N113 (= N35) binding ; mutation to A: Decreased feedback inhibition by valine/isoleucine.
I114 (≠ V36) binding

Sites not aligning to the query:

328 binding
331 G→D: Decreased feedback inhibition by leucine and valine/isoleucine.
332 binding
333 binding
340 F→A: Decreased feedback inhibition by valine/isoleucine.
345 Y→A: No effect.
346 binding ; mutation N->A,H: Decreased feedback inhibition by leucine and increased feedback inhibition by valine/isoleucine.
347 binding

Query Sequence

>17730 FitnessBrowser__Keio:17730
MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPIQDSDKSHIWLLVNDDQ
RLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ

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SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory