SitesBLAST
Comparing 17824 FitnessBrowser__Keio:17824 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P04968 L-threonine dehydratase biosynthetic IlvA; Threonine deaminase; EC 4.3.1.19 from Escherichia coli (strain K12) (see paper)
100% identity, 100% coverage: 1:514/514 of query aligns to 1:514/514 of P04968
- K62 (= K62) modified: N6-(pyridoxal phosphate)lysine
- N89 (= N89) binding
- GGGGL 188:192 (= GGGGL 188:192) binding
- S315 (= S315) binding
1tdjA Threonine deaminase (biosynthetic) from e. Coli (see paper)
97% identity, 99% coverage: 5:514/514 of query aligns to 1:494/494 of 1tdjA
- active site: K58 (= K62), A83 (= A87), E209 (= E213), S213 (= S217), C215 (= C219), G237 (= G241), L310 (= L314), S311 (= S315)
- binding pyridoxal-5'-phosphate: F57 (= F61), K58 (= K62), N85 (= N89), G184 (= G188), G185 (= G189), G186 (= G190), G187 (= G191), G237 (= G241), E282 (= E286), S311 (= S315), G312 (= G316)
2gn2A Crystal structure of tetrameric biodegradative threonine deaminase (tdcb) from salmonella typhimurium in complex with cmp at 2.5a resolution (hexagonal form) (see paper)
38% identity, 58% coverage: 32:328/514 of query aligns to 26:322/326 of 2gn2A
- active site: K56 (= K62), A81 (= A87), Q207 (≠ E213), V211 (≠ S217), G213 (≠ C219), G235 (= G241), I308 (≠ L314), S309 (= S315)
- binding cytidine-5'-monophosphate: R51 (≠ P57), T52 (≠ V58), G53 (≠ H59), A114 (= A120), D117 (≠ G123), Y118 (≠ F124), N312 (= N318)
2zr8A Crystal structure of modified serine racemase complexed with serine (see paper)
34% identity, 58% coverage: 29:326/514 of query aligns to 20:313/319 of 2zr8A
- active site: K53 (= K62), S78 (≠ A87), E204 (= E213), G208 (≠ S217), D210 (≠ C219), G232 (= G241), I303 (≠ L314), S304 (= S315)
- binding magnesium ion: E204 (= E213), G208 (≠ S217), D210 (≠ C219)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F61), K53 (= K62), S77 (= S86), S78 (≠ A87), N80 (= N89), H81 (= H90), P147 (= P156), G179 (= G188), G180 (= G189), G181 (= G190), G182 (= G191), G232 (= G241), E277 (= E286), T279 (≠ S288), S304 (= S315)
- binding serine: S78 (≠ A87), R129 (≠ A138), D231 (≠ E240), G232 (= G241), A233 (≠ V242), Q234 (≠ A243), T235 (≠ V244)
2zpuA Crystal structure of modified serine racemase from s.Pombe. (see paper)
34% identity, 58% coverage: 29:326/514 of query aligns to 20:313/319 of 2zpuA
- active site: K53 (= K62), S78 (≠ A87), E204 (= E213), G208 (≠ S217), D210 (≠ C219), G232 (= G241), I303 (≠ L314), S304 (= S315)
- binding magnesium ion: E204 (= E213), G208 (≠ S217), D210 (≠ C219)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F61), K53 (= K62), S77 (= S86), S78 (≠ A87), N80 (= N89), H81 (= H90), P147 (= P156), G179 (= G188), G180 (= G189), G181 (= G190), G182 (= G191), G232 (= G241), E277 (= E286), T279 (≠ S288), S304 (= S315)
O59791 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 4.3.1.17; EC 4.3.1.18; EC 5.1.1.18 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
34% identity, 58% coverage: 29:326/514 of query aligns to 24:317/323 of O59791
- S82 (≠ A87) mutation to A: Loss of racemase activity. Reduces D-serine dehydratase activity by 99%. Slightly reduced L-serine dehydratase activity.
1wtcA Crystal structure of s.Pombe serine racemase complex with amppcp (see paper)
34% identity, 58% coverage: 29:326/514 of query aligns to 19:312/318 of 1wtcA
- active site: K52 (= K62), S77 (≠ A87), E203 (= E213), G207 (≠ S217), D209 (≠ C219), G231 (= G241), I302 (≠ L314), S303 (= S315)
- binding phosphomethylphosphonic acid adenylate ester: N20 (≠ Q30), K47 (≠ P57), M48 (≠ V58), A109 (≠ D119), A110 (= A120), Y114 (≠ F124)
- binding magnesium ion: E203 (= E213), G207 (≠ S217), D209 (≠ C219)
- binding pyridoxal-5'-phosphate: F51 (= F61), K52 (= K62), N79 (= N89), G178 (= G188), G179 (= G189), G180 (= G190), G181 (= G191), G231 (= G241), E276 (= E286), T278 (≠ S288), S303 (= S315)
1v71A Crystal structure of s.Pombe serine racemase
34% identity, 58% coverage: 29:326/514 of query aligns to 19:312/318 of 1v71A
- active site: K52 (= K62), S77 (≠ A87), E203 (= E213), G207 (≠ S217), D209 (≠ C219), G231 (= G241), I302 (≠ L314), S303 (= S315)
- binding magnesium ion: E203 (= E213), G207 (≠ S217), D209 (≠ C219)
- binding pyridoxal-5'-phosphate: F51 (= F61), K52 (= K62), N79 (= N89), G178 (= G188), G179 (= G189), G180 (= G190), G181 (= G191), G231 (= G241), E276 (= E286), T278 (≠ S288), S303 (= S315), G304 (= G316)
Q7XSN8 Serine racemase; D-serine dehydratase; D-serine ammonia-lyase; L-serine dehydratase; L-serine ammonia-lyase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Oryza sativa subsp. japonica (Rice) (see paper)
35% identity, 56% coverage: 32:321/514 of query aligns to 38:329/339 of Q7XSN8
- E219 (= E213) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-225.
- D225 (= D216) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-219.
A4F2N8 L-threo-3-hydroxyaspartate ammonia-lyase; L-threo-3-hydroxyaspartate dehydratase; L-THA DH; EC 4.3.1.16 from Pseudomonas sp. (see paper)
33% identity, 58% coverage: 29:328/514 of query aligns to 20:315/319 of A4F2N8
- K53 (= K62) mutation to A: Loss of enzymatic activity.
1ve5A Crystal structure of t.Th. Hb8 threonine deaminase
40% identity, 52% coverage: 53:321/514 of query aligns to 41:306/308 of 1ve5A
- active site: K50 (= K62), S56 (≠ A68), S72 (≠ A87), E200 (= E213), A204 (≠ S217), D206 (≠ C219), G229 (= G241), L299 (= L314), S300 (= S315)
- binding calcium ion: E200 (= E213), A204 (≠ S217), D206 (≠ C219)
- binding pyridoxal-5'-phosphate: F49 (= F61), K50 (= K62), N74 (= N89), G175 (= G188), G176 (= G189), G177 (= G190), G178 (= G191), E274 (= E286), T276 (≠ S288), S300 (= S315), G301 (= G316)
5cvcA Structure of maize serine racemase (see paper)
34% identity, 56% coverage: 32:321/514 of query aligns to 22:313/329 of 5cvcA
- active site: K52 (= K62), S77 (≠ A87), E203 (= E213), A207 (= A214), D209 (= D216), G231 (= G241), V306 (≠ L314), S307 (= S315)
- binding magnesium ion: E203 (= E213), A207 (= A214), D209 (= D216)
- binding pyridoxal-5'-phosphate: F51 (= F61), K52 (= K62), N79 (= N89), S178 (≠ G188), G179 (= G189), G180 (= G190), G181 (= G191), L232 (≠ V242), E275 (= E286), S307 (= S315), G308 (= G316)
7nbhAAA structure of human serine racemase in complex with DSiP fragment Z26781964, XChem fragment screen (see paper)
30% identity, 58% coverage: 25:321/514 of query aligns to 16:316/320 of 7nbhAAA
- active site: K53 (= K62), S81 (≠ A87), E207 (= E213), A211 (≠ S217), D213 (≠ A218), G236 (= G241), L309 (= L314), S310 (= S315)
- binding calcium ion: E207 (= E213), A211 (≠ S217), D213 (≠ A218)
- binding N-[(1H-benzimidazol-2-yl)methyl]furan-2-carboxamide: S81 (≠ A87), G85 (≠ A91), Q86 (= Q92), K111 (= K117), I115 (≠ V121), Y118 (≠ F124), D235 (≠ E240), P281 (= P287), N313 (= N318), V314 (= V319), D315 (≠ N320)
7nbgAAA structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
30% identity, 58% coverage: 25:321/514 of query aligns to 16:316/322 of 7nbgAAA
- active site: K53 (= K62), S81 (≠ A87), E207 (= E213), A211 (≠ S217), D213 (≠ A218), G236 (= G241), L309 (= L314), S310 (= S315)
- binding calcium ion: E207 (= E213), A211 (≠ S217), D213 (≠ A218)
- binding pyridoxal-5'-phosphate: F52 (= F61), K53 (= K62), N83 (= N89), G182 (= G188), G183 (= G189), G184 (= G190), G185 (= G191), M186 (≠ L192), G236 (= G241), V237 (= V242), T282 (≠ S288), S310 (= S315), G311 (= G316)
- binding ~{N}-[2-(2-methylphenyl)ethyl]ethanamide: S81 (≠ A87), G85 (≠ A91), Q86 (= Q92), I101 (= I107), K111 (= K117), I115 (≠ V121), Y118 (≠ F124)
7nbfAAA structure of human serine racemase in complex with DSiP fragment Z126932614, XChem fragment screen (see paper)
30% identity, 58% coverage: 25:321/514 of query aligns to 16:316/323 of 7nbfAAA
- active site: K53 (= K62), S81 (≠ A87), E207 (= E213), A211 (≠ S217), D213 (≠ A218), G236 (= G241), L309 (= L314), S310 (= S315)
- binding calcium ion: E207 (= E213), A211 (≠ S217), D213 (≠ A218)
- binding magnesium ion: N244 (≠ E250)
- binding pyridoxal-5'-phosphate: F52 (= F61), K53 (= K62), N83 (= N89), G182 (= G188), G183 (= G189), G184 (= G190), G185 (= G191), M186 (≠ L192), G236 (= G241), V237 (= V242), T282 (≠ S288), S310 (= S315), G311 (= G316)
- binding 2-[(methylsulfonyl)methyl]-1H-benzimidazole: H21 (≠ Q30), L22 (≠ V31), T23 (= T32), P24 (= P33), L26 (≠ Q35), T27 (≠ K36), F46 (≠ R55)
Sites not aligning to the query:
7nbdAAA structure of human serine racemase in complex with DSiP fragment Z235449082, XChem fragment screen (see paper)
30% identity, 58% coverage: 25:321/514 of query aligns to 16:316/323 of 7nbdAAA
- active site: K53 (= K62), S81 (≠ A87), E207 (= E213), A211 (≠ S217), D213 (≠ A218), G236 (= G241), L309 (= L314), S310 (= S315)
- binding calcium ion: E207 (= E213), A211 (≠ S217), D213 (≠ A218)
- binding [4-(1H-benzimidazol-1-yl)phenyl]methanol: W272 (≠ F278), L278 (≠ V284), V314 (= V319), L316 (≠ F321)
- binding magnesium ion: N244 (≠ E250)
- binding pyridoxal-5'-phosphate: F52 (= F61), K53 (= K62), N83 (= N89), G182 (= G188), G183 (= G189), G184 (= G190), G185 (= G191), M186 (≠ L192), G236 (= G241), V237 (= V242), E280 (= E286), T282 (≠ S288), S310 (= S315), G311 (= G316)
Sites not aligning to the query:
7nbcCCC structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
30% identity, 58% coverage: 25:321/514 of query aligns to 16:316/323 of 7nbcCCC
- active site: K53 (= K62), S81 (≠ A87), E207 (= E213), A211 (≠ S217), D213 (≠ A218), G236 (= G241), L309 (= L314), S310 (= S315)
- binding biphenyl-4-ylacetic acid: T78 (= T84), H79 (≠ A85), H84 (= H90), V148 (= V154), H149 (≠ P155), P150 (= P156)
- binding calcium ion: E207 (= E213), A211 (≠ S217), D213 (≠ A218)
- binding pyridoxal-5'-phosphate: F52 (= F61), K53 (= K62), N83 (= N89), G182 (= G188), G183 (= G189), G184 (= G190), G185 (= G191), M186 (≠ L192), G236 (= G241), V237 (= V242), T282 (≠ S288), S310 (= S315), G311 (= G316)
7nbcAAA structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
30% identity, 58% coverage: 25:321/514 of query aligns to 16:316/323 of 7nbcAAA
- active site: K53 (= K62), S81 (≠ A87), E207 (= E213), A211 (≠ S217), D213 (≠ A218), G236 (= G241), L309 (= L314), S310 (= S315)
- binding calcium ion: E207 (= E213), A211 (≠ S217), D213 (≠ A218)
- binding magnesium ion: N244 (≠ E250)
- binding pyridoxal-5'-phosphate: F52 (= F61), K53 (= K62), N83 (= N89), G182 (= G188), G183 (= G189), G184 (= G190), G185 (= G191), M186 (≠ L192), G236 (= G241), V237 (= V242), T282 (≠ S288), S310 (= S315), G311 (= G316)
Sites not aligning to the query:
7nbgDDD structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
31% identity, 58% coverage: 25:320/514 of query aligns to 16:310/310 of 7nbgDDD
- active site: K53 (= K62), S76 (≠ A87), E202 (= E213), A206 (≠ S217), D208 (≠ A218), G231 (= G241), L304 (= L314), S305 (= S315)
- binding calcium ion: E202 (= E213), A206 (≠ S217), D208 (≠ A218)
- binding magnesium ion: N239 (≠ E250)
- binding ortho-xylene: S76 (≠ A87), Q81 (= Q92), I96 (= I107), Y113 (≠ F124)
- binding pyridoxal-5'-phosphate: F52 (= F61), K53 (= K62), N78 (= N89), G177 (= G188), G178 (= G189), G179 (= G190), G180 (= G191), M181 (≠ L192), G231 (= G241), V232 (= V242), E275 (= E286), T277 (≠ S288), S305 (= S315), G306 (= G316)
Sites not aligning to the query:
3l6bA X-ray crystal structure of human serine racemase in complex with malonate a potent inhibitor (see paper)
30% identity, 58% coverage: 25:321/514 of query aligns to 17:312/322 of 3l6bA
- active site: K54 (= K62), S77 (≠ A87), E203 (= E213), A207 (≠ S217), D209 (≠ A218), G232 (= G241), T278 (≠ S288), L305 (= L314), S306 (= S315)
- binding malonate ion: K54 (= K62), S76 (= S86), S77 (≠ A87), N79 (= N89), H80 (= H90), R128 (≠ A138), G232 (= G241)
- binding manganese (ii) ion: E203 (= E213), A207 (≠ S217), D209 (≠ A218)
- binding pyridoxal-5'-phosphate: F53 (= F61), K54 (= K62), N79 (= N89), G178 (= G188), G179 (= G189), G180 (= G190), G181 (= G191), M182 (≠ L192), V233 (= V242), E276 (= E286), T278 (≠ S288), S306 (= S315), G307 (= G316)
Query Sequence
>17824 FitnessBrowser__Keio:17824
MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRLDNVILVKREDRQPVHS
FKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLGVKALIVMPTATADIKVDA
VRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHPMVIAGQGTLALELLQQDAHL
DRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSACLKAALDAGHPVDLPRVGLFAE
GVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKDLFEDVRAVAEPSGALALAGMKKYI
ALHNIRGERLAHILSGANVNFHGLRYVSERCELGEQREALLAVTIPEEKGSFLKFCQLLG
GRSVTEFNYRFADAKNACIFVGVRLSRGLEERKEILQMLNDGGYSVVDLSDDEMAKLHVR
YMVGGRPSHPLQERLYSFEFPESPGALLRFLNTLGTYWNISLFHYRSHGTDYGRVLAAFE
LGDHEPDFETRLNELGYDCHDETNNPAFRFFLAG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory