SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 17840 FitnessBrowser__Keio:17840 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P61887 Glucose-1-phosphate thymidylyltransferase 2; G1P-TT 2; dTDP-glucose pyrophosphorylase 2; dTDP-glucose synthase 2; EC 2.7.7.24 from Escherichia coli (strain K12) (see paper)
100% identity, 100% coverage: 1:293/293 of query aligns to 1:293/293 of P61887

query
sites
P61887

M

M

K

K

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L

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D
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Y

D108 (= D108) binding
D223 (= D223) binding

1mc3A Crystal structure of rffh (see paper)
100% identity, 99% coverage: 1:290/293 of query aligns to 2:291/291 of 1mc3A

query
sites
1mc3A

M

M

K

K

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L

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P

binding magnesium ion: D109 (= D108), D224 (= D223)
binding thymidine-5'-triphosphate: L7 (= L6), G9 (= G8), G10 (= G9), G12 (= G11), T13 (= T12), R14 (= R13), K24 (= K23), Q25 (= Q24), Q81 (= Q80), P84 (= P83), L87 (= L86), D109 (= D108)

5ifyA Crystal structure of glucose-1-phosphate thymidylyltransferase from burkholderia vietnamiensis in complex with 2 -deoxyuridine-5'- monophosphate and 2'-deoxy-thymidine-b-l-rhamnose
65% identity, 97% coverage: 2:286/293 of query aligns to 3:287/293 of 5ifyA

query
sites
5ifyA

K

K

G

G

I

I

L
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L

A

A

G
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G

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G

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S

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G

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T

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D

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A

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F

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N

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T

N

G

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Y

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Q

Y

Y

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L

L

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E

N

L

L

binding 2'-deoxy-thymidine-beta-l-rhamnose: L7 (= L6), G9 (= G8), Q81 (= Q80), P84 (= P83), G86 (= G85), L87 (= L86), D109 (= D108), Y144 (≠ F143), G145 (= G144), E160 (= E159), K161 (= K160), V171 (= V170), Y175 (= Y174), R193 (= R192)
binding 2'-deoxyuridine 5'-monophosphate: Y113 (≠ F112), G114 (= G113), H115 (≠ Q114), D116 (≠ G115), G217 (= G216), R218 (= R217), G219 (= G218), E254 (= E253), I255 (= I254)

4b2xB Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
66% identity, 97% coverage: 2:286/293 of query aligns to 13:297/302 of 4b2xB

query
sites
4b2xB

K

K

G

G

I

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

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D

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A

F

F

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F

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G

G

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E

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L

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C

A

L

L

V

V

L

L

G

G

D

D

N

N

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L

F
x
Y

F

Y

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G

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H

G

D

F

F

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E

K

L

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H

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L

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P

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R

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Y

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F

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D

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N

G

F

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A

A

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I

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S

L

L

E

E

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K

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E

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P

K

K

S

S

N

N

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Y

A

A

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T

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G

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L

Y

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F

F

Y

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D

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K

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L

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K

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G

G

E

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A

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A

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F

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K

N

R

R

Q

Q

G

G

F

L

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K

I

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A

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C

C

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E

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E
|
E

I

I

A

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W

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R

R

N
x
Q

G

K

W

W

L

I

D

D

D

A

E

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G

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V

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K

E

R

K

A

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A

A

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A

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P

L

L

A

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

L

K

E

R

L

L

binding 6-amino-1-butyl-5-(cyclopentylamino)pyrimidine-2,4(1H,3H)-dione: L54 (= L43), Y122 (≠ F111), E264 (= E253), Q269 (≠ N258)

3zllA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
66% identity, 97% coverage: 2:286/293 of query aligns to 8:292/297 of 3zllA

query
sites
3zllA

K

K

G

G

I

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

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K

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Q

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L

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D

K

K

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M

M

I

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Y

Y

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P

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T

L

L

M

M

L
|
L

A

A

G

G

I

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E

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I

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D

K

T

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R

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F

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Q

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L

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D

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F

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L

L

E

Q

Y

Y

A

A

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V

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Q

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P

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S

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P

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D

G

G

L

L

A

A

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Q

A

A

F

F

I

L

I

I

G

G

E

E

T

S

F

F

L

I

N

G

G

N

E

D

P

L

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S

C

A

L

L

V

V

L

L

G

G

D

D

N

N

I

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F

Y

G

G

Q

H

G

D

F

F

S

H

P

E

K

L

L

L

R

G

H

S

V

A

A

S

A

Q

R

R

T

Q

E

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

Q

H

V

V

M

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

D

Q

N

G

F

G

R

K

A

A

I

I

S

S

L

L

E

E

K

K

P

P

K

L

Q

E

P

P

K

K

S

S

N

N

W

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

S

Q

K

Q

V

V

E

D

Y

I

A

A

K

R

Q

D

V

L

K

K

P

P

S

S

E

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

S

D

I

V

N

N

Q

R

M

A

Y

Y

L

L

E

E

A

R

G

G

N

Q

L

L

T

S

V

V

E

E

L

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

I

L

E

E

A

A

S

G

T

Q

F

F

V

I

Q

A

T

T

V

L

E

E

K

N

R

R

Q

Q

G

G

F

L

K

K

I

V

A

A

C

C

L

P

E

E

E
|
E

I
|
I

A

A

W

Y

R
|
R

N

Q

G

K

W

W

L

I

D

D

D

A

E

A

G

Q

V

L

K

E

R

K

A

L

A

A

S

A

S

P

L

L

A

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

L

K

E

R

L

L

binding 5-azanyl-6-oxidanyl-1-(phenylmethyl)pyrimidine-2,4-dione: L49 (= L43), E259 (= E253), I260 (= I254), R263 (= R257)

3zlkA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
66% identity, 97% coverage: 2:286/293 of query aligns to 8:292/297 of 3zlkA

query
sites
3zlkA

K

K

G

G

I

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

I

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S
|
S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

E

E

I

I

L

L

I

I

T

S

T

T

P

P

E

Q

D

D

K

T

G

P

Y

R

F

F

Q

Q

R

Q

L

L

G

G

D

D

G

G

S

S

E

N

F

W

G

G

I

L

Q

D

L

L

E

Q

Y

Y

A

A

E

V

Q

Q

P

P

S

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

T

S

F

F

L

I

N

G

G

N

E

D

P

L

S

S

C

A

L

L

V

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

G
|
G

Q

H

G

D

F

F

S

H

P

E

K

L

L

L

R

G

H

S

V

A

A

S

A

Q

R

R

T

Q

E

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

Q

H

V

V

M

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

D

Q

N

G

F

G

R

K

A

A

I

I

S

S

L

L

E

E

K

K

P

P

K

L

Q

E

P

P

K

K

S

S

N

N

W

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

S

Q

K

Q

V

V

E

D

Y

I

A

A

K

R

Q

D

V

L

K

K

P

P

S

S

E

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

S

D

I

V

N

N

Q

R

M

A

Y

Y

L

L

E

E

A

R

G

G

N

Q

L

L

T

S

V

V

E

E

L

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

I

L

E

E

A

A

S

G

T

Q

F

F

V

I

Q

A

T

T

V

L

E

E

K

N

R

R

Q

Q

G

G

F

L

K

K

I
x
V

A
|
A

C

C

L

P

E

E

E
|
E

I
|
I

A

A

W

Y

R

R

N

Q

G

K

W

W

L

I

D

D

D

A

E

A

G

Q

V

L

K

E

R

K

A

L

A

A

S

A

S

P

L

L

A

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

L

K

E

R

L

L

binding n-(6-amino-1-benzyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)benzenesulfonamide: S45 (= S39), L49 (= L43), Y117 (≠ F111), Y118 (≠ F112), G119 (= G113), V254 (≠ I248), A255 (= A249), E259 (= E253), I260 (= I254)

4ho9A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with udp-galactose and utp
66% identity, 97% coverage: 1:285/293 of query aligns to 1:285/294 of 4ho9A

query
sites
4ho9A

M

M

K

K

G

G

I

I

L
|
L

A

S

G
|
G

S

S

G

G

T

T

R

R

L

L

H

Y

P

P

I

L

T

T

R

K

G

V

V

V

S

S

K

K

Q

Q

L

L

P

P

I

V

Y

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

L

L

S

S

V

V

L

L

M

M

L
|
L

A

A

G

G

I

I

R

K

E

D

I

I

L

L

I

I

T

S

T

T

P

P

E

E

D

D

K

T

G

P

Y

R

F

F

Q

E

R

Q

L

L

G

G

D

G

G

G

S

S

E

E

F

L

G

G

I

I

Q

S

L

L

E

S

Y

Y

A

A

E

V

Q
|
Q

P

S

S

S

P
|
P

D

D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

T

E

F

F

L

I

N

G

G

D

E

D

P

N

S

V

C

A

L

L

V

V

L
|
L

G

G

D
|
D

N

N

I

I

F

F

F

Y

G
|
G

Q
x
H

G
|
G

F

F

S
x
T

P

E

K

L

L

L

R

Q

H

R

V

A

A

A

A

N

R

R

T

K

E

S

G

G

A

A

T

T

V

I

F

F

G

G

Y

Y

Q

N

V

V

M

K

D

D

P

P

E

Q

R

R

F

F

G
|
G

V

V

E

E

F

F

D

D

D

E

N

K

F

G

R

K

A

V

I

I

S

S

L

I

E

E

E
|
E

K
|
K

P

P

K

E

Q

E

P

P

K

K

S

S

N

S

W

Y

A

A

V
|
V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

S

N

K

R

V

V

E

D

Y

I

A

A

K

K

Q

N

V

I

K

T

P

P

S

S

E

A

R
|
R

G

G

E

E

L

L

E

E

I

I

T

T

S

D

I

V

N

N

Q

K

M

A

Y

Y

L

L

E

E

A

L

G

G

N

E

L

L

T

H

V

V

E

E

L

L

G
|
G

R
|
R

G
|
G

F

F

A

A

W
|
W

L

L

D

D

T

T

G

G

T

T

H

H

D

E

S

S

L

L

I

L

E

Q

A

A

S

S

T

Q

F

F

V

I

Q

E

T

T

V

I

E

E

K

K

R

R

Q

Q

G

S

F

L

K

K

I

V

A

A

C

C

L

L

E

E

I
|
I

A

A

W

Y

R

R

N

M

G

G

W

Y

L

I

D

S

D

R

E

E

G

Q

V

L

K

I

R

K

A

L

A

A

S

E

S

P

L

L

A

M

K

K

T

N

G

E

Y

Y

G

G

Q

Q

Y

Y

L

L

L

M

E

N

L

L

binding galactose-uridine-5'-diphosphate: L6 (= L6), G8 (= G8), G9 (= G9), Q80 (= Q80), P83 (= P83), G85 (= G85), L86 (= L86), L106 (= L106), D108 (= D108), G144 (= G144), E159 (= E159), K160 (= K160), V170 (= V170), R192 (= R192), W221 (= W221)
binding uridine 5'-triphosphate: L43 (= L43), G113 (= G113), H114 (≠ Q114), G115 (= G115), T117 (≠ S117), G216 (= G216), R217 (= R217), G218 (= G218), I254 (= I254)

5fuhA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor
66% identity, 97% coverage: 2:286/293 of query aligns to 9:293/298 of 5fuhA

query
sites
5fuhA

K

K

G

G

I

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

I

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

V
x
T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

E

E

I

I

L

L

I

I

T

S

T

T

P

P

E

Q

D

D

K

T

G

P

Y

R

F

F

Q

Q

R

Q

L

L

G

G

D

D

G

G

S

S

E

N

F

W

G

G

I

L

Q

D

L

L

E

Q

Y

Y

A

A

E

V

Q

Q

P

P

S

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

T

S

F

F

L

I

N

G

G

N

E

D

P

L

S

S

C

A

L

L

V

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

F

Y

G
|
G

Q

H

G

D

F
|
F

S

H

P

E

K

L

L

L

R

G

H

S

V

A

A

S

A

Q

R

R

T

Q

E

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

Q

H

V

V

M

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

D

Q

N

G

F

G

R

K

A

A

I

I

S

S

L

L

E

E

K

K

P

P

K

L

Q

E

P

P

K

K

S

S

N

N

W

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

S

Q

K

Q

V

V

E

D

Y

I

A

A

K

R

Q

D

V

L

K

K

P

P

S

S

E

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

S

D

I

V

N

N

Q

R

M

A

Y

Y

L

L

E

E

A

R

G

G

N

Q

L

L

T

S

V

V

E

E

L

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

I

L

E

E

A

A

S

G

T

Q

F

F

V

I

Q

A

T

T

V

L

E

E

K

N

R

R

Q

Q

G

G

F

L

K

K

I

V

A
|
A

C

C

L

P

E

E

I

I

A

A

W

Y

R
|
R

N
x
Q

G

K

W

W

L

I

D

D

D

A

E

A

G

Q

V

L

K

E

R

K

A

L

A

A

S

A

S

P

L

L

A

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

L

K

E

R

L

L

binding N-[6-amino-1-(3-bromobenzyl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl]-N-methylbenzenesulfonamide: T47 (≠ V40), L50 (= L43), Y118 (≠ F111), G120 (= G113), F123 (= F116), A256 (= A249), R264 (= R257), Q265 (≠ N258)

4b4mA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
66% identity, 97% coverage: 2:286/293 of query aligns to 7:291/296 of 4b4mA

query
sites
4b4mA

K

K

G

G

I

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

I

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S
|
S

V
x
T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

E

E

I

I

L

L

I

I

T

S

T

T

P

P

E

Q

D

D

K

T

G

P

Y

R

F

F

Q

Q

R

Q

L

L

G

G

D

D

G

G

S

S

E

N

F

W

G

G

I

L

Q

D

L

L

E

Q

Y

Y

A

A

E

V

Q

Q

P

P

S

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

T

S

F

F

L

I

N

G

G

N

E

D

P

L

S

S

C

A

L

L

V

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

F

Y

G
|
G

Q

H

G

D

F

F

S

H

P

E

K

L

L

L

R

G

H

S

V

A

A

S

A

Q

R

R

T

Q

E

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

Q

H

V

V

M

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

D

Q

N

G

F

G

R

K

A

A

I

I

S

S

L

L

E

E

K

K

P

P

K

L

Q

E

P

P

K

K

S

S

N

N

W

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

S

Q

K

Q

V

V

E

D

Y

I

A

A

K

R

Q

D

V

L

K

K

P

P

S

S

E

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

S

D

I

V

N

N

Q

R

M

A

Y

Y

L

L

E

E

A

R

G

G

N

Q

L

L

T

S

V

V

E

E

L

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

I

L

E

E

A

A

S

G

T

Q

F

F

V

I

Q

A

T

T

V

L

E

E

K

N

R

R

Q

Q

G

G

F

L

K

K

I
x
V

A
|
A

C

C

L

P

E

E

E
|
E

I
|
I

A

A

W

Y

R
|
R

N

Q

G

K

W

W

L

I

D

D

D

A

E

A

G

Q

V

L

K

E

R

K

A

L

A

A

S

A

S

P

L

L

A

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

L

K

E

R

L

L

binding N-(6-amino-1-benzyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-4-fluoro-N-methylbenzenesulfonamide: S44 (= S39), T45 (≠ V40), L48 (= L43), Y116 (≠ F111), G118 (= G113), V253 (≠ I248), A254 (= A249), E258 (= E253), I259 (= I254), R262 (= R257)

4b4bA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
66% identity, 97% coverage: 2:286/293 of query aligns to 9:293/298 of 4b4bA

query
sites
4b4bA

K

K

G

G

I

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

I

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

V

T

L

L

M

M

L

L

A

A

G

G

I

I

R

R

E

E

I

I

L

L

I

I

T

S

T

T

P

P

E

Q

D

D

K

T

G

P

Y

R

F

F

Q

Q

R

Q

L

L

G

G

D

D

G

G

S

S

E

N

F

W

G

G

I

L

Q

D

L

L

E

Q

Y

Y

A

A

E

V

Q

Q

P

P

S

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

T

S

F

F

L

I

N

G

G

N

E

D

P

L

S

S

C

A

L

L

V

V

L

L

G

G

D

D

N

N

I

L

F

Y

G

G

Q

H

G

D

F

F

S

H

P

E

K

L

L

L

R

G

H

S

V

A

A

S

A

Q

R

R

T

Q

E

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

Q

H

V

V

M

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

D

Q

N

G

F

G

R

K

A

A

I

I

S

S

L

L

E

E

K

K

P

P

K

L

Q

E

P

P

K

K

S

S

N

N

W

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

S

Q

K

Q

V

V

E

D

Y

I

A

A

K

R

Q

D

V

L

K

K

P

P

S

S

E

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

S

D

I

V

N

N

Q

R

M

A

Y

Y

L

L

E

E

A

R

G

G

N

Q

L

L

T

S

V

V

E

E

L

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

I

L

E

E

A

A

S

G

T

Q

F

F

V

I

Q

A

T

T

V

L

E

E

K

N

R

R

Q

Q

G

G

F

L

K

K

I
x
V

A
|
A

C

C

L

P

E

E

E
|
E

I
|
I

A

A

W

Y

R
|
R

N

Q

G

K

W

W

L

I

D

D

D

A

E

A

G

Q

V

L

K

E

R

K

A

L

A

A

S

A

S

P

L

L

A

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

L

K

E

R

L

L

binding 1-(2-phenylethyl)pyrimidine-2,4,6(1H,3H,5H)-trione: V255 (≠ I248), A256 (= A249), E260 (= E253), I261 (= I254), R264 (= R257)

4ho4A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with thymidine and glucose-1-phosphate
66% identity, 97% coverage: 1:285/293 of query aligns to 1:285/289 of 4ho4A

query
sites
4ho4A

M

M

K

K

G

G

I

I

L

L

A

S

G

G

S

S

G

G

T

T

R

R

L

L

H

Y

P

P

I

L

T

T

R

K

G

V

V

V

S

S

K

K

Q

Q

L

L

P

P

I

V

Y

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

L

L

S

S

V

V

L

L

M

M

L

L

A

A

G

G

I

I

R

K

E

D

I

I

L

L

I

I

T

S

T

T

P

P

E

E

D

D

K

T

G

P

Y

R

F

F

Q

E

R

Q

L

L

G

G

D

G

G

G

S

S

E

E

F

L

G

G

I

I

Q

S

L

L

E

S

Y

Y

A

A

E

V

Q

Q

P

S

S

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

T

E

F

F

L

I

N

G

G

D

E

D

P

N

S

V

C

A

L

L

V

V

L
|
L

G

G

D

D

N

N

I

I

F

F

F

Y

G

G

Q

H

G

G

F

F

S

T

P

E

K

L

L

L

R

Q

H

R

V

A

A

A

A

N

R

R

T

K

E

S

G

G

A

A

T

T

V

I

F

F

G

G

Y

Y

Q

N

V

V

M

K

D

D

P

P

E

Q

R

R

F
|
F

G
|
G

V

V

E

E

F

F

D

D

D

E

N

K

F

G

R

K

A

V

I

I

S

S

L

I

E

E

E
|
E

K
|
K

P

P

K

E

Q

E

P

P

K

K

S

S

N

S

W

Y

A

A

V
|
V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

S

N

K

R

V

V

E

D

Y

I

A

A

K

K

Q

N

V

I

K

T

P

P

S

S

E

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

S

D

I

V

N

N

Q

K

M

A

Y

Y

L

L

E

E

A

L

G

G

N

E

L

L

T

H

V

V

E

E

L

L

G

G

R

R

G

G

F

F

A

A

W
|
W

L

L

D

D

T

T

G

G

T

T

H

H

D

E

S

S

L

L

I

L

E

Q

A

A

S

S

T

Q

F

F

V

I

Q

E

T

T

V

I

E

E

K

K

R

R

Q

Q

G

S

F

L

K

K

I

V

A

A

C

C

L

L

E

E

I

I

A

A

W

Y

R

R

N

M

G

G

W

Y

L

I

D

S

D

R

E

E

G

Q

V

L

K

I

R

K

A

L

A

A

S

E

S

P

L

L

A

M

K

K

T

N

G

E

Y

Y

G

G

Q

Q

Y

Y

L

L

L

M

E

N

L

L

binding 1-O-phosphono-alpha-D-glucopyranose: L106 (= L106), F143 (= F143), G144 (= G144), E159 (= E159), K160 (= K160), V170 (= V170), W221 (= W221)

5fyeA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor
66% identity, 97% coverage: 2:286/293 of query aligns to 7:291/296 of 5fyeA

query
sites
5fyeA

K

K

G

G

I

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

I

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S
|
S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

E

E

I

I

L

L

I

I

T

S

T

T

P

P

E

Q

D

D

K

T

G

P

Y

R

F

F

Q

Q

R

Q

L

L

G

G

D

D

G

G

S

S

E

N

F

W

G

G

I

L

Q

D

L

L

E

Q

Y

Y

A

A

E

V

Q

Q

P

P

S

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

T

S

F

F

L

I

N

G

G

N

E

D

P

L

S

S

C

A

L

L

V

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

F

Y

G
|
G

Q

H

G

D

F

F

S

H

P

E

K

L

L

L

R

G

H

S

V

A

A

S

A

Q

R

R

T

Q

E

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

Q

H

V

V

M

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

D

Q

N

G

F

G

R

K

A

A

I

I

S

S

L

L

E

E

K

K

P

P

K

L

Q

E

P

P

K

K

S

S

N

N

W

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

S

Q

K

Q

V

V

E

D

Y

I

A

A

K

R

Q

D

V

L

K

K

P

P

S

S

E

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

S

D

I

V

N

N

Q

R

M

A

Y

Y

L

L

E

E

A

R

G

G

N

Q

L

L

T

S

V

V

E

E

L

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

I

L

E

E

A

A

S

G

T

Q

F

F

V

I

Q

A

T

T

V

L

E

E

K

N

R

R

Q

Q

G

G

F

L

K

K

I
x
V

A
|
A

C

C

L

P

E

E

E
|
E

I

I

A

A

W

Y

R
|
R

N

Q

G

K

W

W

L

I

D

D

D

A

E

A

G

Q

V

L

K

E

R

K

A

L

A

A

S

A

S

P

L

L

A

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

L

K

E

R

L

L

binding N-(6-Amino-1-(3-fluorobenzyl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylbenzenesulfonamide: S44 (= S39), L48 (= L43), Y116 (≠ F111), G118 (= G113), V253 (≠ I248), A254 (= A249), E258 (= E253), R262 (= R257)

5fu0A Pseudomonas aeruginosa rmla in complex with allosteric inhibitor
66% identity, 97% coverage: 2:286/293 of query aligns to 7:291/296 of 5fu0A

query
sites
5fu0A

K

K

G

G

I

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

I

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S
|
S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

E

E

I

I

L

L

I

I

T

S

T

T

P

P

E

Q

D

D

K

T

G

P

Y

R

F

F

Q

Q

R

Q

L

L

G

G

D

D

G

G

S

S

E

N

F

W

G

G

I

L

Q

D

L

L

E

Q

Y

Y

A

A

E

V

Q

Q

P

P

S

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

T

S

F

F

L

I

N

G

G

N

E

D

P

L

S

S

C

A

L

L

V

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

G
|
G

Q

H

G

D

F

F

S
x
H

P

E

K

L

L

L

R

G

H

S

V

A

A

S

A

Q

R

R

T

Q

E

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

Q

H

V

V

M

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

D

Q

N

G

F

G

R

K

A

A

I

I

S

S

L

L

E

E

K

K

P

P

K

L

Q

E

P

P

K

K

S

S

N

N

W

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

S

Q

K

Q

V

V

E

D

Y

I

A

A

K

R

Q

D

V

L

K

K

P

P

S

S

E

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

S

D

I

V

N

N

Q

R

M

A

Y

Y

L

L

E

E

A

R

G

G

N

Q

L

L

T

S

V

V

E

E

L

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

I

L

E

E

A

A

S

G

T

Q

F

F

V

I

Q

A

T

T

V

L

E

E

K

N

R

R

Q

Q

G

G

F

L

K

K

I
x
V

A
|
A

C

C

L

P

E

E

E
|
E

I

I

A

A

W

Y

R

R

N
x
Q

G

K

W

W

L

I

D

D

D

A

E

A

G

Q

V

L

K

E

R

K

A

L

A

A

S

A

S

P

L

L

A

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

L

K

E

R

L

L

binding n-(6-amino-1-(3-methylbenzyl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-n-methylbenzenesulfonamide: S44 (= S39), L48 (= L43), Y116 (≠ F111), Y117 (≠ F112), G118 (= G113), H122 (≠ S117), V253 (≠ I248), A254 (= A249), E258 (= E253), Q263 (≠ N258)

5ftvA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor
66% identity, 97% coverage: 2:286/293 of query aligns to 7:291/296 of 5ftvA

query
sites
5ftvA

K

K

G

G

I

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

I

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S
|
S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

E

E

I

I

L

L

I

I

T

S

T

T

P

P

E

Q

D

D

K

T

G

P

Y

R

F

F

Q

Q

R

Q

L

L

G

G

D

D

G

G

S

S

E

N

F

W

G

G

I

L

Q

D

L

L

E

Q

Y

Y

A

A

E

V

Q

Q

P

P

S

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

T

S

F

F

L

I

N

G

G

N

E

D

P

L

S

S

C

A

L

L

V

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

G
|
G

Q

H

G

D

F
|
F

S

H

P

E

K

L

L

L

R

G

H

S

V

A

A

S

A

Q

R

R

T

Q

E

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

Q

H

V

V

M

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

D

Q

N

G

F

G

R

K

A

A

I

I

S

S

L

L

E

E

K

K

P

P

K

L

Q

E

P

P

K

K

S

S

N

N

W

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

S

Q

K

Q

V

V

E

D

Y

I

A

A

K

R

Q

D

V

L

K

K

P

P

S

S

E

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

S

D

I

V

N

N

Q

R

M

A

Y

Y

L

L

E

E

A

R

G

G

N

Q

L

L

T

S

V

V

E

E

L

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

I

L

E

E

A

A

S

G

T

Q

F

F

V

I

Q

A

T

T

V

L

E

E

K

N

R

R

Q

Q

G

G

F

L

K

K

I
x
V

A
|
A

C

C

L

P

E

E

E
|
E

I

I

A

A

W

Y

R
|
R

N
x
Q

G

K

W

W

L

I

D

D

D

A

E

A

G

Q

V

L

K

E

R

K

A

L

A

A

S

A

S

P

L

L

A

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

L

K

E

R

L

L

binding n-(6-amino-1-(4-bromobenzyl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-n-methylbenzenesulfonamide: S44 (= S39), L48 (= L43), Y116 (≠ F111), Y117 (≠ F112), G118 (= G113), F121 (= F116), V253 (≠ I248), A254 (= A249), E258 (= E253), R262 (= R257), Q263 (≠ N258)

5ftsA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor
66% identity, 97% coverage: 2:286/293 of query aligns to 7:291/296 of 5ftsA

query
sites
5ftsA

K

K

G

G

I

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

I

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S
|
S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

E

E

I

I

L

L

I

I

T

S

T

T

P

P

E

Q

D

D

K

T

G

P

Y

R

F

F

Q

Q

R

Q

L

L

G

G

D

D

G

G

S

S

E

N

F

W

G

G

I

L

Q

D

L

L

E

Q

Y

Y

A

A

E

V

Q

Q

P

P

S

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

T

S

F

F

L

I

N

G

G

N

E

D

P

L

S

S

C

A

L

L

V

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

F

Y

G
|
G

Q

H

G

D

F

F

S
x
H

P

E

K

L

L

L

R

G

H

S

V

A

A

S

A

Q

R

R

T

Q

E

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

Q

H

V

V

M

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

D

Q

N

G

F

G

R

K

A

A

I

I

S

S

L

L

E

E

K

K

P

P

K

L

Q

E

P

P

K

K

S

S

N

N

W

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

S

Q

K

Q

V

V

E

D

Y

I

A

A

K

R

Q

D

V

L

K

K

P

P

S

S

E

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

S

D

I

V

N

N

Q

R

M

A

Y

Y

L

L

E

E

A

R

G

G

N

Q

L

L

T

S

V

V

E

E

L

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

I

L

E

E

A

A

S

G

T

Q

F

F

V

I

Q

A

T

T

V

L

E

E

K

N

R

R

Q

Q

G

G

F

L

K

K

I
x
V

A
|
A

C

C

L

P

E

E

E
|
E

I
|
I

A

A

W

Y

R

R

N

Q

G

K

W

W

L

I

D

D

D

A

E

A

G

Q

V

L

K

E

R

K

A

L

A

A

S

A

S

P

L

L

A

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

L

K

E

R

L

L

binding N-(6-Amino-1-(2-bromobenzyl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylbenzenesulfonamide: S44 (= S39), L48 (= L43), Y116 (≠ F111), G118 (= G113), H122 (≠ S117), V253 (≠ I248), A254 (= A249), E258 (= E253), I259 (= I254)

5fu8A Pseudomonas aeruginosa rmla in complex with allosteric inhibitor
66% identity, 97% coverage: 2:286/293 of query aligns to 8:292/297 of 5fu8A

query
sites
5fu8A

K

K

G

G

I

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

I

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S
|
S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

E

E

I

I

L

L

I

I

T

S

T

T

P

P

E

Q

D

D

K

T

G

P

Y

R

F

F

Q

Q

R

Q

L

L

G

G

D

D

G

G

S

S

E

N

F

W

G

G

I

L

Q

D

L

L

E

Q

Y

Y

A

A

E

V

Q

Q

P

P

S

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

T

S

F

F

L

I

N

G

G

N

E

D

P

L

S

S

C

A

L

L

V

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

G
|
G

Q

H

G

D

F

F

S
x
H

P

E

K

L

L

L

R

G

H

S

V

A

A

S

A

Q

R

R

T

Q

E

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

Q

H

V

V

M

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

D

Q

N

G

F

G

R

K

A

A

I

I

S

S

L

L

E

E

K

K

P

P

K

L

Q

E

P

P

K

K

S

S

N

N

W

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

S

Q

K

Q

V

V

E

D

Y

I

A

A

K

R

Q

D

V

L

K

K

P

P

S

S

E

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

S

D

I

V

N

N

Q

R

M

A

Y

Y

L

L

E

E

A

R

G

G

N

Q

L

L

T

S

V

V

E

E

L

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

I

L

E

E

A

A

S

G

T

Q

F

F

V

I

Q

A

T

T

V

L

E

E

K

N

R

R

Q

Q

G

G

F

L

K

K

I
x
V

A

A

C

C

L

P

E

E

E
|
E

I

I

A

A

W

Y

R
|
R

N
x
Q

G

K

W

W

L

I

D

D

D

A

E

A

G

Q

V

L

K

E

R

K

A

L

A

A

S

A

S

P

L

L

A

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

L

K

E

R

L

L

binding N-(6-amino-1-(4-bromo-3-methylbenzyl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylbenzenesulfonamide: S45 (= S39), L49 (= L43), Y117 (≠ F111), Y118 (≠ F112), G119 (= G113), H123 (≠ S117), V254 (≠ I248), E259 (= E253), R263 (= R257), Q264 (≠ N258)

4b2wA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
66% identity, 97% coverage: 2:286/293 of query aligns to 8:292/297 of 4b2wA

query
sites
4b2wA

K

K

G

G

I

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

I

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S
|
S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

E

E

I

I

L

L

I

I

T

S

T

T

P

P

E

Q

D

D

K

T

G

P

Y

R

F

F

Q

Q

R

Q

L

L

G

G

D

D

G

G

S

S

E

N

F

W

G

G

I

L

Q

D

L

L

E

Q

Y

Y

A

A

E

V

Q

Q

P

P

S

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

T

S

F

F

L

I

N

G

G

N

E

D

P

L

S

S

C

A

L

L

V

V

L

L

G

G

D

D

N

N

I

L

F

Y

G

G

Q

H

G

D

F

F

S

H

P

E

K

L

L

L

R

G

H

S

V

A

A

S

A

Q

R

R

T

Q

E

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

Q

H

V

V

M

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

D

Q

N

G

F

G

R

K

A

A

I

I

S

S

L

L

E

E

K

K

P

P

K

L

Q

E

P

P

K

K

S

S

N

N

W

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

S

Q

K

Q

V

V

E

D

Y

I

A

A

K

R

Q

D

V

L

K

K

P

P

S

S

E

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

S

D

I

V

N

N

Q

R

M

A

Y

Y

L

L

E

E

A

R

G

G

N

Q

L

L

T

S

V

V

E

E

L

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

I

L

E

E

A

A

S

G

T

Q

F

F

V

I

Q

A

T

T

V

L

E

E

K

N

R

R

Q

Q

G

G

F

L

K

K

I

V

A

A

C

C

L

P

E

E

E
|
E

I
|
I

A

A

W

Y

R
|
R

N

Q

G

K

W

W

L

I

D

D

D

A

E

A

G

Q

V

L

K

E

R

K

A

L

A

A

S

A

S

P

L

L

A

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

L

K

E

R

L

L

binding N-(6-amino-1-benzyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)benzamide: S45 (= S39), L49 (= L43), E259 (= E253), I260 (= I254), R263 (= R257)

4asjA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
66% identity, 97% coverage: 2:286/293 of query aligns to 3:287/292 of 4asjA

query
sites
4asjA

K

K

G

G

I

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

I

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S
|
S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

E

E

I

I

L

L

I

I

T

S

T

T

P

P

E

Q

D

D

K

T

G

P

Y

R

F

F

Q

Q

R

Q

L

L

G

G

D

D

G

G

S

S

E

N

F

W

G

G

I

L

Q

D

L

L

E

Q

Y

Y

A

A

E

V

Q

Q

P

P

S

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

T

S

F

F

L

I

N

G

G

N

E

D

P

L

S

S

C

A

L

L

V

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

F

Y

G
|
G

Q

H

G

D

F
|
F

S

H

P

E

K

L

L

L

R

G

H

S

V

A

A

S

A

Q

R

R

T

Q

E

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

Q

H

V

V

M

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

D

Q

N

G

F

G

R

K

A
|
A

I

I

S

S

L

L

E

E

K

K

P

P

K

L

Q

E

P

P

K

K

S

S

N

N

W

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

S

Q

K

Q

V

V

E

D

Y

I

A

A

K

R

Q

D

V

L

K

K

P

P

S

S

E

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

S

D

I

V

N

N

Q

R

M

A

Y

Y

L

L

E

E

A

R

G

G

N

Q

L

L

T

S

V
|
V

E

E

L

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

I

L

E

E

A

A

S

G

T

Q

F

F

V

I

Q

A

T

T

V

L

E

E

K

N

R

R

Q

Q

G

G

F

L

K

K

I
x
V

A

A

C

C

L

P

E

E

E
|
E

I

I

A

A

W

Y

R

R

N

Q

G

K

W

W

L

I

D

D

D

A

E

A

G

Q

V

L

K

E

R

K

A

L

A

A

S

A

S

P

L

L

A

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

L

K

E

R

L

L

binding N-(6-amino-1-benzyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylbenzenesulfonamide: S40 (= S39), L44 (= L43), Y112 (≠ F111), G114 (= G113), F117 (= F116), V249 (≠ I248), E254 (= E253)
binding phosphate ion: A155 (= A154), V213 (= V212)

1g3lA The structural basis of the catalytic mechanism and regulation of glucose-1-phosphate thymidylyltransferase (rmla). Tdp-l-rhamnose complex. (see paper)
66% identity, 97% coverage: 2:286/293 of query aligns to 3:287/292 of 1g3lA

query
sites
1g3lA

K

K

G

G

I

I

L
|
L

A

A

G
|
G

S

S

G

G

T

T

R

R

L

L

H

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

I

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

V

T

L

L

M

M

L

L

A

A

G

G

I

I

R

R

E

E

I

I

L

L

I

I

T

S

T

T

P

P

E

Q

D

D

K

T

G

P

Y

R

F

F

Q

Q

R

Q

L

L

G

G

D

D

G

G

S

S

E

N

F

W

G

G

I

L

Q

D

L

L

E

Q

Y

Y

A

A

E

V

Q
|
Q

P

P

S

S

P
|
P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

T

S

F

F

L

I

N

G

G

N

E

D

P

L

S

S

C

A

L

L

V

V

L

L

G

G

D
|
D

N

N

I

L

F

Y

F
x
Y

G
|
G

Q
x
H

G
x
D

F
|
F

S
x
H

P

E

K

L

L

L

R

G

H

S

V

A

A

S

A

Q

R

R

T

Q

E

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

Q

H

V

V

M

L

D

D

P

P

E

E

R

R

F
x
Y

G
|
G

V

V

E

E

F

F

D

D

D

Q

N

G

F

G

R

K

A

A

I

I

S

S

L

L

E

E

E
|
E

K
|
K

P

P

K

L

Q

E

P

P

K

K

S

S

N

N

W

Y

A

A

V
|
V

T
|
T

G
|
G

L

L

Y
|
Y

F

F

Y

Y

D

D

S

Q

K

Q

V

V

E

D

Y

I

A

A

K

R

Q

D

V

L

K

K

P

P

S

S

E

P

R
|
R

G

G

E

E

L

L

E

E

I

I

T

T

S

D

I

V

N

N

Q

R

M

A

Y

Y

L

L

E

E

A

R

G

G

N

Q

L

L

T

S

V

V

E

E

L

I

L

M

G
|
G

R
|
R

G
|
G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

I

L

E

E

A

A

S

G

T

Q

F

F

V

I

Q

A

T

T

V

L

E

E

K

N

R

R

Q

Q

G

G

F

L

K

K

I

V

A

A

C

C

L

P

E

E

E
|
E

I
|
I

A

A

W

Y

R
|
R

N

Q

G

K

W

W

L

I

D

D

D

A

E

A

G

Q

V

L

K

E

R

K

A

L

A

A

S

A

S

P

L

L

A

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

L

K

E

R

L

L

binding 2'-deoxy-thymidine-beta-l-rhamnose: L7 (= L6), G9 (= G8), G10 (= G9), Q81 (= Q80), P84 (= P83), D109 (= D108), Y113 (≠ F112), G114 (= G113), H115 (≠ Q114), D116 (≠ G115), F117 (= F116), H118 (≠ S117), Y144 (≠ F143), G145 (= G144), E160 (= E159), K161 (= K160), V171 (= V170), T172 (= T171), G173 (= G172), Y175 (= Y174), R193 (= R192), G217 (= G216), R218 (= R217), G219 (= G218), E254 (= E253), I255 (= I254), R258 (= R257)

1g2vA The structural basis of the catalytic mechanism and regulation of glucose-1-phosphate thymidylyltransferase (rmla). Ttp complex. (see paper)
66% identity, 97% coverage: 2:286/293 of query aligns to 3:287/292 of 1g2vA

query
sites
1g2vA

K

K

G

G

I

I

L
|
L

A
|
A

G
|
G

S

S

G
|
G

T
|
T

R
|
R

L

L

H

H

P

P

I

A

T

T

R

L

G

A

V

I

S

S

K

K

Q
|
Q

L

L

P

P

I

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

V

T

L

L

M

M

L

L

A

A

G

G

I

I

R

R

E

E

I

I

L

L

I

I

T

S

T

T

P

P

E

Q

D

D

K

T

G

P

Y

R

F

F

Q

Q

R

Q

L

L

G

G

D

D

G

G

S

S

E

N

F

W

G

G

I

L

Q

D

L

L

E

Q

Y

Y

A

A

E

V

Q
|
Q

P

P

S

S

P
|
P

D

D

G

G

L
|
L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

T

S

F

F

L

I

N

G

G

N

E

D

P

L

S

S

C

A

L

L

V

V

L

L

G

G

D
|
D

N

N

I

L

F

Y

F
x
Y

G
|
G

Q
x
H

G
x
D

F
|
F

S
x
H

P

E

K

L

L

L

R

G

H

S

V

A

A

S

A

Q

R

R

T

Q

E

T

G

G

A

A

T

S

V

V

F

F

G

A

Y

Y

Q

H

V

V

M

L

D

D

P

P

E

E

R

R

F

Y

G

G

V

V

E

E

F

F

D

D

D

Q

N

G

F

G

R

K

A

A

I

I

S

S

L

L

E

E

K

K

P

P

K

L

Q

E

P

P

K

K

S

S

N

N

W

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

S

Q

K

Q

V

V

E

D

Y

I

A

A

K

R

Q

D

V

L

K

K

P

P

S

S

E

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

S

D

I

V

N

N

Q

R

M

A

Y

Y

L

L

E

E

A

R

G

G

N

Q

L

L

T

S

V

V

E

E

L

I

L

M

G
|
G

R
|
R

G
|
G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

I

L

E

E

A

A

S

G

T

Q

F

F

V

I

Q

A

T

T

V

L

E

E

K

N

R

R

Q

Q

G

G

F

L

K

K

I

V

A

A

C

C

L

P

E

E

E
|
E

I
|
I

A

A

W

Y

R

R

N

Q

G

K

W

W

L

I

D

D

D

A

E

A

G

Q

V

L

K

E

R

K

A

L

A

A

S

A

S

P

L

L

A

A

K

K

T

N

G

G

Y

Y

G

G

Q

Q

Y

Y

L

L

L

K

E

R

L

L

binding thymidine-5'-triphosphate: L7 (= L6), A8 (= A7), G9 (= G8), G10 (= G9), G12 (= G11), T13 (= T12), R14 (= R13), Q25 (= Q24), Q81 (= Q80), P84 (= P83), L87 (= L86), D109 (= D108), Y113 (≠ F112), G114 (= G113), H115 (≠ Q114), D116 (≠ G115), F117 (= F116), H118 (≠ S117), G217 (= G216), R218 (= R217), G219 (= G218), E254 (= E253), I255 (= I254)

Query Sequence

>17840 FitnessBrowser__Keio:17840
MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLAGIREILIITTPEDKGY
FQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPSCLVLGDNIFFGQGFSPKL
RHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEKPKQPKSNWAVTGLYFYDSKV
VEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRGFAWLDTGTHDSLIEASTFVQTV
EKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKTGYGQYLLELLRARPRQY

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory