SitesBLAST

Q7WTT9 Arsenate respiratory reductase iron-sulfur subunit ArrB; Arsenate respiratory reductase small subunit; ARR small subunit from Shewanella sp. (strain ANA-3) (see paper)
34% identity, 81% coverage: 35:214/223 of query aligns to 1:225/234 of Q7WTT9

query
sites
Q7WTT9

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C12 (= C46) binding
C15 (= C49) binding
C18 (= C52) binding
C22 (= C56) binding
C57 (= C92) binding
C60 (= C95) binding
C65 (= C100) binding
C69 (= C104) binding
C89 (= C125) binding
C92 (= C128) binding
C95 (= C131) binding
C99 (= C135) binding
C164 (= C152) binding
C167 (= C155) binding
C179 (= C168) binding
C183 (= C172) binding

2ivfB Ethylbenzene dehydrogenase from aromatoleum aromaticum (see paper)
34% identity, 62% coverage: 78:215/223 of query aligns to 122:259/337 of 2ivfB

query
sites
2ivfB

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binding fe3-s4 cluster: C148 (= C104), T150 (= T106), I153 (≠ S109), C169 (= C125), K170 (≠ V126), G171 (= G127), H172 (≠ C128), R173 (≠ Q129), H174 (≠ Y130), C175 (= C131), S193 (≠ A149)
binding protoporphyrin ix containing fe: T150 (= T106), K170 (≠ V126), H172 (≠ C128)
binding iron/sulfur cluster: M135 (≠ S91), C136 (= C92), N137 (≠ Q93), H138 (= H94), C139 (= C95), P142 (≠ A98), C144 (= C100), V162 (= V118), C179 (= C135), A183 (≠ V139), I184 (≠ R140), K195 (= K151), C196 (= C152), I197 (≠ D153), L198 (≠ F154), C199 (= C155), N209 (≠ P166), C211 (= C168), C215 (= C172), V219 (≠ A176), R220 (≠ L177)

Sites not aligning to the query:

binding iron/sulfur cluster: 13, 14, 15, 16, 17, 19, 23, 27, 38, 39, 41

5ch7B Crystal structure of the perchlorate reductase pcrab - phe164 gate switch intermediate - from azospira suillum ps (see paper)
35% identity, 62% coverage: 78:215/223 of query aligns to 119:256/329 of 5ch7B

query
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5ch7B

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binding fe3-s4 cluster: C145 (= C104), I150 (≠ S109), C166 (= C125), K167 (≠ V126), G168 (= G127), A169 (≠ C128), Q170 (= Q129), A171 (≠ Y130), C172 (= C131), A190 (= A149)
binding iron/sulfur cluster: P130 (≠ R89), C133 (= C92), N134 (≠ Q93), C136 (= C95), P139 (≠ A98), C141 (= C100), C176 (= C135), C193 (= C152), G195 (≠ F154), C196 (= C155), C208 (= C168), C212 (= C172), V213 (≠ P173), G214 (≠ T174)

Sites not aligning to the query:

binding iron/sulfur cluster: 17, 18, 19, 20, 21, 23, 27, 31, 43, 45

P18776 Anaerobic dimethyl sulfoxide reductase chain B; DMSO reductase iron-sulfur subunit from Escherichia coli (strain K12) (see 2 papers)
34% identity, 81% coverage: 35:215/223 of query aligns to 3:185/205 of P18776

query
sites
P18776

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C102 (= C128) mutation C->F,S,W,Y: Loss of electron transfer from menaquinol to DMSO.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P0AAJ3 Formate dehydrogenase, nitrate-inducible, iron-sulfur subunit; Anaerobic formate dehydrogenase iron-sulfur subunit; Formate dehydrogenase-N subunit beta; FDH-N subunit beta from Escherichia coli (strain K12) (see paper)
31% identity, 70% coverage: 42:196/223 of query aligns to 35:199/294 of P0AAJ3

query
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P0AAJ3

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C39 (= C46) binding
C42 (= C49) binding
C45 (= C52) binding
C49 (= C56) binding
C100 (= C92) binding
C103 (= C95) binding
C108 (= C100) binding
C112 (= C104) binding
C133 (= C125) binding
C136 (= C128) binding
C139 (= C131) binding
C143 (= C135) binding
C160 (= C152) binding
C163 (= C155) binding
C175 (= C168) binding
C179 (= C172) binding

1kqfB Formate dehydrogenase n from e. Coli (see paper)
31% identity, 70% coverage: 42:196/223 of query aligns to 34:198/289 of 1kqfB

query
sites
1kqfB

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R

binding protoporphyrin ix containing fe: Y137 (= Y130)
binding iron/sulfur cluster: C38 (= C46), I39 (= I47), G40 (= G48), C41 (= C49), K42 (≠ T50), C44 (= C52), C48 (= C56), N52 (= N60), T77 (= T70), M79 (≠ I72), C99 (= C92), M100 (≠ Q93), H101 (= H94), C102 (= C95), P105 (≠ A98), C107 (= C100), C111 (= C104), P112 (= P105), I117 (≠ F110), V125 (= V118), C132 (= C125), I133 (≠ V126), G134 (= G127), C135 (= C128), G136 (≠ Q129), Y137 (= Y130), C138 (= C131), C142 (= C135), I146 (≠ V139), P147 (≠ R140), V156 (≠ A149), K158 (= K151), C159 (= C152), L161 (≠ F154), C162 (= C155), P172 (= P166), C174 (= C168), C178 (= C172), P179 (= P173), I183 (≠ L177)

Sites not aligning to the query:

6lodB Cryo-em structure of the air-oxidized photosynthetic alternative complex iii from roseiflexus castenholzii (see paper)
29% identity, 80% coverage: 33:211/223 of query aligns to 679:914/929 of 6lodB

query
sites
6lodB

N

N

G

G

V

Y

R

K

Y

W

G

G

M

M

V

S

H

I

D

D

E

L

S

N

L

V

C
|
C

I
x
N

G
x
S

C
|
C

T
x
N

A
|
A

C
|
C

M

V

D

V

A

A

C
|
C

R

Q

E

S

V

E

N
|
N

K

N

V

I

P

P

E

V

G

V

V

G

S

K

R

D

L

E

T

V

I

W

I

L

R

G

S

R

E

E

P

M

Q

H

G
x
W

E

I

F

R

P
x
I

D

D

V

Q

K

Y

Y

Y

R

V

F

G

F

D

R

E

K

H

S

T

-

P

-

N

-

V

-

Y

-

N

-

M

-

V

-

M

-
x
L

C
|
C

Q
|
Q

H
x
Q

C
|
C

D

E

H

H

A

A

P
|
P

C
|
C

V

E

D

I

V
|
V

C
|
C

P
|
P

T

V

G

A

A

A

S

T

F

V

R

H

D

D

A

-

A

A

S

E

G

G

I

L

V
x
N

D

N

V

M

N

V

P

Y

D
x
N

L
x
R

C
|
C

V
|
V

G
|
G

C
x
T

Q

K

Y

Y

C
|
C

I

S

A

N

C
|
C

P

P

Y
|
Y

R

K

V
|
V

R
|
R

-

R

-

F

-

N

F

F

I

L

H

Q

-

Y

-

Q

-

D

-

V

-

P

-

Y

-

R

-

S

-

P

-

I

-

D

-

A

-

S

-

T

-

E

-

N

-

D

-

S

-

I

P

P

V

V

T

L

K

K

-

M

-

R

-

N

-

P

-

D

-

V

-

T

-

V

-

R

-

A

-

R

-

G

T

V

A
x
M

D

E

K
|
K

C
|
C

D

T

F
|
F

C
|
C

-

V

-

Q

-

R

-

I

R

N

K

E

T

A

N
x
R

L
x
I

Q

Q

A

A

G
x
R

K

T

-

E

-

N

-

R

-

I

-

A

-

D

-

G

-

E

-

I

L

M

P
x
T

A

A

C
|
C

V

Q

E

Q

A

V

C
|
C

P

P

T

T

K

Q

A

A

L
x
I

T

V

F

F

G

G

N

D

L

L

D

N

D

D

P

P

N

Q

S

A

E

R

I

V

S

V

Q

D

L

L

L

K

R

E

Q

Q

K

P

P

L

T

K

Y

Y

R

T

Y

S

K

L

L

D

A

K

L

L

G

N

T

T

K

K

P

P

K

R

L

V

binding fe3-s4 cluster: V758 (= V103), C759 (= C104), P760 (= P105), C779 (= C125), V780 (= V126), G781 (= G127), T782 (≠ C128), C785 (= C131), M838 (≠ A149)
binding heme c: N777 (≠ D123), R778 (≠ L124), R852 (≠ N159), I853 (≠ L160), R856 (≠ G163)
binding iron/sulfur cluster: C692 (= C46), N693 (≠ I47), S694 (≠ G48), C695 (= C49), N696 (≠ T50), A697 (= A51), C698 (= C52), C702 (= C56), N706 (= N60), W724 (≠ G78), I727 (≠ P81), L746 (vs. gap), C747 (= C92), Q748 (= Q93), Q749 (≠ H94), C750 (= C95), P754 (= P99), C755 (= C100), N772 (≠ V118), C789 (= C135), Y791 (= Y137), V793 (= V139), R794 (= R140), K840 (= K151), C841 (= C152), F843 (= F154), C844 (= C155), T869 (≠ P166), C871 (= C168), C875 (= C172), I880 (≠ L177)

6f0kB Alternative complex iii (see paper)
28% identity, 78% coverage: 36:210/223 of query aligns to 723:944/961 of 6f0kB

query
sites
6f0kB

R

Q

Y

W

G

A

M

M

V

V

H

I

D

D

E

L

S

N

L

A

C
|
C

I
x
T

G
|
G

C
|
C

T
x
N

A
|
A

C
|
C

M

I

D

V

A

A

C
|
C

R

D

E

S

V

E

N

N

K

N

V

I

P

P

-

M

-

V

-

G

-

K

-

N

-

E

-

V

-

G

-

R

-

G

E

R

G

E

V

M

S

H

R
x
W

L

L

T

R

I
|
I

I

D

R

R

-

Y

-

F

-

V

-

S

S

D

E

E

P

A

Q

H

G

A

E

D

F

D

P

P

D

Q

V

I

K

V

Y

V

R

Q

F

P

F

V

R

-

K

-

S

P

C
|
C

Q
x
M

H
|
H

C
|
C

D

E

H

N

A

A

P
|
P

C
|
C

V

E

D

S

V

V

C
|
C

P

P

T
x
V

G

A

A

A

S

T

F

V

R

H

D

-

A

S

A

P

S

D

G

G

I

L

V
x
N

D

E

V
x
M

N

V

P

Y

D
x
N

L
x
R

C
|
C

V
x
I

G
|
G

C

T

Q

R

Y

Y

C
|
C

I

S

A

N

C
|
C

P

P

Y

Y

R

K

V

V

R

R

F

R

I

F

H

N

-

W

-

F

-

N

-

W

-

V

-

K

-

T

-

L

P

P

V

I

T

Q

-

V

-

Q

-

M

-

A

-

Q

-

N

-

P

-

D

-

V

-

T

-

V

-

R

-

F

-

R

K

G

T

V

A
x
M

D

E

K

K

C
|
C

D

T

F
x
Y

C
|
C

-

V

-

Q

-

R

-

I

-

R

-

E

-

A

-

Q

R
|
R

K

Q

T

A

N
|
N

L

I

Q

E

A

K

G

R

K

P

L

L

-

R

-

D

-

G

-

E

-

V

-

K

P

T

A
|
A

C
|
C

V

Q

E

Q

A

A

C
|
C

P

P

T

A

K

E

A

A

L

I

T

T

F

F

G

G

N

D

L

L

D

N

D

D

P

P

N

N

S

N

E

A

I

V

S

V

Q

K

L

Q

L

R

R

Q

Q

N

K

A

P

R

T

R

Y

Y

R

E

Y

M

K

L

L

A

A

A

L

L

G

N

T

V

K

K

P

P

K

R

binding fe3-s4 cluster: C803 (= C104), V805 (≠ T106), M818 (≠ V120), C823 (= C125), I824 (≠ V126), G825 (= G127), C829 (= C131), M869 (≠ A149)
binding heme c: N821 (≠ D123), R822 (≠ L124), R884 (= R156), N887 (= N159)
binding iron/sulfur cluster: C733 (= C46), T734 (≠ I47), G735 (= G48), C736 (= C49), N737 (≠ T50), A738 (= A51), C739 (= C52), C743 (= C56), W765 (≠ R68), I768 (= I71), C791 (= C92), M792 (≠ Q93), H793 (= H94), C794 (= C95), P798 (= P99), C799 (= C100), N816 (≠ V118), C833 (= C135), C872 (= C152), Y874 (≠ F154), C875 (= C155), A901 (= A167), C902 (= C168), C906 (= C172)

Sites not aligning to the query:

binding heme c: 719

8cm6D W-formate dehydrogenase c872a from desulfovibrio vulgaris - with formamide (see paper)
33% identity, 63% coverage: 42:181/223 of query aligns to 7:165/214 of 8cm6D

query
sites
8cm6D

D

D

E

L

S

S

L

R

C
|
C

I
x
T

G
x
A

C
|
C

T
x
R

A

G

C
|
C

M

Q

D

I

A

A

C
|
C

R

K

E

Q

V

W

N
x
K

K

N

V

L

P

P

E

A

G

E

V

E

S

T

R

R

-

N

-

T

-

G

-

S

-

H

-

Q

-

N

-

P

-

D

-

L

-

S

-

Y

-

V

-

T

L

L

T
x
K

I

T

I

V

R

R

-

F

S

T

E

E

P

K

Q

S

G
x
R

E

K

F

G

P
|
P

D

G

V

I

K

D

Y

W

R

L

F

F

R

P

K

E

S

Q

C
|
C

Q
x
R

H

H

C
|
C

D

V

H

E

A
x
P

P
|
P

C
|
C

-

K

-

G

-

Q

V

A

D

D

V

V

C

D

P

L

T

E

G

G

A

A

S

V

F

V

R

K

D

D

A

E

A

T

S

T

G

G

I

A

V
|
V

D

L

V

F

N

T

P

E

D

L

L

T

C

A

-

K

V

V

G

D

C

G

Q

E

Y

S

C

V

I

R

A

S

A

A

C
|
C

P

P

Y

Y

R

D

V
x
I

R
x
P

F

R

I

I

H

D

P

P

V

V

T

T

K

K

T

R

A

L

D

S

K
|
K

C
|
C

D
|
D

F
x
M

C
|
C

R

N

K

D

T

-

N

R

L

V

Q

Q

A

N

G

G

K

L

L

L

P

P

A

A

C
|
C

V

V

E

K

A

T

C
|
C

P
|
P

T
|
T

K

G

A
x
T

L
x
M

T

N

F

F

G

G

N

D

binding formamide: R59 (≠ G78), P62 (= P81)
binding iron/sulfur cluster: C11 (= C46), T12 (≠ I47), A13 (≠ G48), C14 (= C49), R15 (≠ T50), C17 (= C52), C21 (= C56), K25 (≠ N60), K50 (≠ T70), C73 (= C92), R74 (≠ Q93), C76 (= C95), P79 (≠ A98), P80 (= P99), C81 (= C100), V102 (= V118), C120 (= C135), I124 (≠ V139), P125 (≠ R140), K136 (= K151), C137 (= C152), D138 (= D153), M139 (≠ F154), C140 (= C155), C152 (= C168), C156 (= C172), P157 (= P173), T158 (= T174), T160 (≠ A176), M161 (≠ L177)

Sites not aligning to the query:

binding iron/sulfur cluster: 4

8bqgB W-formate dehydrogenase from desulfovibrio vulgaris - soaking with formate 1 min (see paper)
33% identity, 63% coverage: 42:181/223 of query aligns to 7:165/214 of 8bqgB

query
sites
8bqgB

D

D

E

L

S

S

L

R

C
|
C

I
x
T

G
x
A

C
|
C

T
x
R

A

G

C
|
C

M

Q

D

I

A

A

C
|
C

R

K

E

Q

V

W

N
x
K

K

N

V

L

P

P

E

A

G

E

V

E

S

T

R

R

-

N

-

T

-

G

-

S

-

H

-

Q

-

N

-

P

-

D

-

L

-

S

-

Y

-

V

-

T

L

L

T
x
K

I

T

I

V

R

R

-

F

S

T

E

E

P

K

Q

S

G

R

E

K

F

G

P

P

D

G

V

I

K

D

Y

W

R

L

F

F

R

P

K

E

S

Q

C
|
C

Q
x
R

H

H

C
|
C

D

V

H

E

A

P

P
|
P

C
|
C

-

K

-

G

-

Q

V

A

D

D

V

V

C

D

P

L

T

E

G

G

A

A

S

V

F

V

R

K

D

D

A

E

A

T

S

T

G

G

I

A

V
|
V

D

L

V

F

N

T

P

E

D

L

L

T

C

A

-

K

V

V

G

D

C

G

Q

E

Y

S

C

V

I

R

A

S

A

A

C
|
C

P

P

Y

Y

R

D

V
x
I

R
x
P

F

R

I

I

H

D

P

P

V

V

T

T

K

K

T

R

A

L

D

S

K
|
K

C
|
C

D

D

F
x
M

C
|
C

R

N

K

D

T

-

N

R

L

V

Q

Q

A

N

G

G

K

L

L

L

P

P

A

A

C
|
C

V

V

E

K

A

T

C
|
C

P
|
P

T
|
T

K

G

A
x
T

L
x
M

T

N

F

F

G

G

N

D

binding iron/sulfur cluster: C11 (= C46), T12 (≠ I47), A13 (≠ G48), C14 (= C49), R15 (≠ T50), C17 (= C52), C21 (= C56), K25 (≠ N60), K50 (≠ T70), C73 (= C92), R74 (≠ Q93), C76 (= C95), P80 (= P99), C81 (= C100), V102 (= V118), C120 (= C135), I124 (≠ V139), P125 (≠ R140), K136 (= K151), C137 (= C152), M139 (≠ F154), C140 (= C155), C152 (= C168), C156 (= C172), P157 (= P173), T158 (= T174), T160 (≠ A176), M161 (≠ L177)

Sites not aligning to the query:

binding iron/sulfur cluster: 4

7qv7B Cryo-em structure of hydrogen-dependent co2 reductase. (see paper)
31% identity, 63% coverage: 39:179/223 of query aligns to 7:161/183 of 7qv7B

query
sites
7qv7B

M

V

V

V

H

A

D

N

E

P

S

A

L

K

C
|
C

I

I

G
|
G

C
|
C

T
x
K

A
|
A

C
|
C

M

E

D

V

A

A

C
|
C

R

F

E

A

V

V

-
x
H

-

N

-

R

-

N

N

N

K

H

V

V

P

G

E

A

G

T

V

V

S

G

R

T

L

V

T

S

I

I

I

-

R

P

S

V

E

I

P

P

Q
x
R

G

L

E

H

F

L

P

I

D

K

V

T

K

E

Y

H

R

G

F

T

F

M

R

P

K

I

S

Q

C
|
C

Q
x
R

H
|
H

C
|
C

D

E

H

D

A
|
A

P

P

C
|
C

V

A

D

N

V

V

C
|
C

P

T

T

V

G

G

A
|
A

S
x
I

F

K

R

R

D

E

A

G

A

N

S

A

G

I

I

V

V

V

D

D

V

-

N

-

P

E

D

K

L

L

C
|
C

V
x
I

G

G

C
|
C

Q

K

Y

S

C
|
C

I

L

A

L

A

A

C
|
C

P

P

Y

F

-

G

-

A

-
x
I

-

E

-

L

-

P

-

Q

-

Y

-

E

-

D

-

G

-

R

-

E

-

V

-

F

-

Q

-

I

R

N

V

L

R

K

F

L

I

V

H

Q

P

E

V

P

T

R

K

I

T

I

A

A

D

Y

K

K

C
|
C

D

D

F
x
L

C
|
C

R

N

K

D

T

L

N

G

L

-

Q

-

A

-

G

-

K

-

L

E

P
|
P

A

A

C
|
C

V

V

E

K

A

A

C
|
C

P
|
P

T

E

K

N

A

A

L
|
L

T

T

F

L

binding iron/sulfur cluster: C14 (= C46), G16 (= G48), C17 (= C49), K18 (≠ T50), A19 (= A51), C20 (= C52), C24 (= C56), H28 (vs. gap), R48 (≠ Q77), C63 (= C92), R64 (≠ Q93), H65 (= H94), C66 (= C95), A69 (= A98), C71 (= C100), C75 (= C104), A79 (= A108), I80 (≠ S109), C94 (= C125), I95 (≠ V126), C97 (= C128), C100 (= C131), C104 (= C135), I109 (vs. gap), C139 (= C152), L141 (≠ F154), C142 (= C155), P148 (= P166), C150 (= C168), C154 (= C172), P155 (= P173), L159 (= L177)

6btmB Structure of alternative complex iii from flavobacterium johnsoniae (wild type) (see paper)
25% identity, 80% coverage: 34:212/223 of query aligns to 681:941/948 of 6btmB

query
sites
6btmB

G

G

V

H

R

H

Y

F

G

N

M

L

V

S

H

I

D

D

E

L

S

N

L

A

C

C

I

T

G

G

C

C

T

G

A

A

C

C

M

V

D

I

A

A

C

C

R

H

E

A

V

E

N

N

K

N

V

V

P

P

-

V

-

G

-

K

-

A

-

E

-

V

-

R

-

S

-

R

-

D

-

M

-

H

-

W

-

L

-

R

-

I

-

D

-

R

-

Y

-

S

-

E

-

S

-

T

-

F

-

E

-

G

-

D

-

N

-

E

-

R

-

K

E

E

G

G

V

I

S

A

R

G

L

L

-

S

-

L

-

S

T

T

I

F

I

N

R

E

S

M

E

E

P

K

Q

P

G

G

E

D

F

N

P

P

D

Q

V

V

K

A

Y

F

R

Q

F

P

F

V

R

M

K

-

S

-

C
|
C

Q
|
Q

H

H

C
|
C

D

N

H

H

A

A

P

P

C
|
C

V

E

D

T

V

V

C
|
C

P

P

T

V

G

A

A

A

S

T

F

-

R

S

D

H

A

G

A

R

S

Q

G

G

I

Q

V
x
N

D

H

V

M

N

A

P

Y

D

N

L

R

C
|
C

V
|
V

G

G

C
x
T

Q

R

Y

Y

C
|
C

I

A

A

N

C
|
C

P

P

Y

Y

R

K

V

V

R
|
R

-

R

-

F

-

N

-

W

F

F

I

L

H

Y

P

N

V

K

T

N

K

S

T

E

A

F

D

D

-

Y

-

H

-

M

-

N

-

D

-

L

-

G

-

R

-

M

-

V

-

L

-

N

-

P

-

D

-

V

-

N

-

V

-

R

-

S

-

R

-

G

-

V

-
x
M

-

E

K

K

C

C

D

S

F

F

C

C

R

I

K

Q

T

S

N

T

-

Q

-

A

-

V

-

I

L

L

Q

E

A

A

G

K

K

R

L

Q

P

G

A

R

C

V

V

V

-

G

-

K

-

D

-

E

-

F

-

N

-

A

-

C

-

A

-

C

-

S

E

A

A

A

C

C

P

S

T

S

K

G

A

A

L

M

T

V

F

F

G

G

N

D

L

V

D

N

D

D

P

K

N

E

S

S

E

E

I

V

S

A

Q

K

L

L

L

A

R

E

Q

S

K

E

P

R

T

M

Y

Y

R

H

Y

L

K

L

L

E

A

H

L

V

G

G

T

T

K

K

P

P

K

N

L

V

Y

F

binding fe3-s4 cluster: C789 (= C104), C809 (= C125), V810 (= V126), T812 (≠ C128), C815 (= C131), M862 (vs. gap)
binding iron/sulfur cluster: C777 (= C92), Q778 (= Q93), C780 (= C95), C785 (= C100), N802 (≠ V118), C819 (= C135), R824 (= R140)

Sites not aligning to the query:

binding (2R)-3-{[(2R)-2-(decanoylamino)-3-hydroxypropyl]sulfanyl}propane-1,2-diyl ditetradecanoate: 1, 2

8c0zC Cryoem structure of a tungsten-containing aldehyde oxidoreductase from aromatoleum aromaticum (see paper)
32% identity, 64% coverage: 42:183/223 of query aligns to 8:137/158 of 8c0zC

query
sites
8c0zC

D

D

E

P

S

A

L

K

C
|
C

I
x
T

G

G

C
|
C

T

L

A

Q

C
|
C

M

E

D

M

A

A

C
|
C

-

S

-

Y

R

E

E

H

V

T

N

G

K

V

V

I

P

N

E

P

G

S

V

K

S

S

R

R

L

I

T

K

I

V

I

F

R

S

S

F

E

E

P

H

Q

E

G

G

E

-

F

-

P

-

D

-

V

-

K

-

Y

-

R

R

F

K

F

V

R

P

K

Y

S

T

C
|
C

Q

T

H

Q

C
|
C

D

T

H

E

A

A

P

W

C
|
C

V

L

D

H

V

S

C
|
C

P

P

T

V

G

D

A

A

S
x
I

F

R

R

L

D

D

A

L

A

T

S

T

G

G

I

A

V

K

D

M

V

V

N

F

P

E

D

D

L

T

C
|
C

V

V

G

G

C
|
C

Q

K

Y

V

C
|
C

I

T

A

I

A

A

C
|
C

P

P

Y

F

R

G

V
x
T

R

I

F

N

I

Y

H

N

P

Q

V

D

T

T

K

G

T

K

A

V

D

Q

K
|
K

C
|
C

D

D

F

L

C
|
C

R

E

K

G

T

D

N

-

L

-

Q

-

A

-

G

-

K

-

L

-

P

P

A

A

C
|
C

V

A

E

K

A

A

C
|
C

P

P

T
|
T

K

A

A

A

L

I

T

T

F

Y

G

I

N

D

L

A

D

D

binding iron/sulfur cluster: C12 (= C46), T13 (≠ I47), C15 (= C49), C18 (= C52), C22 (= C56), C53 (= C92), C56 (= C95), C61 (= C100), C65 (= C104), I70 (≠ S109), C86 (= C125), C89 (= C128), C92 (= C131), C96 (= C135), T100 (≠ V139), K112 (= K151), C113 (= C152), C116 (= C155), C122 (= C168), C126 (= C172), T128 (= T174)

3egwB The crystal structure of the narghi mutant narh - c16a
31% identity, 47% coverage: 79:182/223 of query aligns to 171:273/509 of 3egwB

query
sites
3egwB

E

Q

F

F

P

E

D

N

V

T

K

F

Y

M

R

M

F

Y

F

L

R
x
P

K

R

S

L

C
|
C

Q
x
E

H
|
H

C
|
C

D

L

H

N

A
x
P

P

A

C
|
C

V

V

D

A

V

T

C
|
C

P

P

T
x
S

G

G

A

A

S
x
I

F

Y

R

K

D

R

A

E

S

D

G

G

I

I

V

V

D

L

V

I

N

D

P

Q

D

D

L

K

C
|
C

V
x
R

G
|
G

C
x
W

Q
x
R

Y

M

C
|
C

I

I

A

T

A

G

C
|
C

P

P

Y

Y

R

K

V

K

R
x
I

F

Y

I

F

H

N

P

W

V

K

T

S

K

G

T

K

A
x
S

D

E

K
|
K

C
|
C

D

I

F
|
F

C
|
C

R

Y

K

-

T

P

N

R

L

I

Q

E

A

A

G

G

K

Q

L

P

P
x
T

A

V

C
|
C

V

S

E

E

A

T

C
|
C

P

V

T
x
G

K

R

A

I

L
x
R

T

Y

F

L

G

G

N

V

L

L

binding fe3-s4 cluster: P181 (≠ R89), C196 (= C104), S198 (≠ T106), I201 (≠ S109), C217 (= C125), R218 (≠ V126), G219 (= G127), W220 (≠ C128), R221 (≠ Q129), C223 (= C131), S241 (≠ A149), C263 (= C172), G265 (≠ T174), R268 (≠ L177)
binding protoporphyrin ix containing fe: W220 (≠ C128), R221 (≠ Q129)
binding iron/sulfur cluster: C184 (= C92), E185 (≠ Q93), H186 (= H94), C187 (= C95), P190 (≠ A98), C192 (= C100), C227 (= C135), I232 (≠ R140), K243 (= K151), C244 (= C152), F246 (= F154), C247 (= C155), T257 (≠ P166), C259 (= C168)

Sites not aligning to the query:

binding fe3-s4 cluster: 17, 18, 19, 20, 22
binding protoporphyrin ix containing fe: 88, 89
binding iron/sulfur cluster: 26, 30, 41, 42

P11349 Respiratory nitrate reductase 1 beta chain; Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta; EC 1.7.5.1 from Escherichia coli (strain K12) (see 2 papers)
31% identity, 47% coverage: 79:182/223 of query aligns to 171:273/512 of P11349

query
sites
P11349

E

Q

F

F

P

E

D

N

V

T

K

F

Y

M

R

M

F

Y

F

L

R

P

K

R

S

L

C
|
C

Q

E

H

H

C
|
C

D

L

H

N

A

P

P

A

C
|
C

V

V

D

A

V

T

C
|
C

P

P

T

S

G

G

A

A

S

I

F

Y

R

K

D

R

A

E

S

D

G

G

I

I

V

V

D

L

V

I

N

D

P

Q

D

D

L

K

C
|
C

V

R

G

G

C

W

Q

R

Y

M

C
|
C

I

I

A

T

A

G

C
|
C

P

P

Y

Y

R

K

V

K

R

I

F

Y

I

F

H

N

P

W

V

K

T

S

K

G

T

K

A

S

D

E

K

K

C
|
C

D

I

F

F

C
|
C

R

Y

K

-

T

P

N

R

L

I

Q

E

A

A

G

G

K

Q

L

P

P

T

A

V

C
|
C

V

S

E

E

A

T

C
|
C

P

V

T

G

K

R

A

I

L

R

T

Y

F

L

G

G

N

V

L

L

C184 (= C92) binding
C187 (= C95) binding
C192 (= C100) binding
C196 (= C104) binding
C217 (= C125) binding
C223 (= C131) binding
C227 (= C135) binding
C244 (= C152) binding
C247 (= C155) binding
C259 (= C168) binding
C263 (= C172) binding

Sites not aligning to the query:

16 binding
19 binding
22 binding
26 binding

7b04A of Nitrite oxidoreductase (Nxr) from the anammox bacterium Kuenenia stuttgartiensis.
27% identity, 87% coverage: 26:219/223 of query aligns to 118:310/409 of 7b04A

query
sites
7b04A

V

V

A

A

K

K

T

K

L

K

N

N

I

I

N

N

G

Q

V

W

R

A

Y

V

G

G

M

Y

V

I

H

P

D

E

E

D

S

K

L

E

C

W

I

R

G

S

C

P

T

N

A

F

C

G

M

E

D

D

A

T

C

A

R

K

E

S

V

S

N

N

K

Q

V

-

P

P

E

G

G

E

V

Y

S

S

R

T

L

L

T

-

I

-

R

-

S

-

E

-

P

-

Q

-

G

-

E

-

F

-

P

P

D

E

V

H

K

S

Y

R

R

W

F

F

-

Y

-

L

R
x
Q

K

R

S
x
I

C
|
C

Q
x
N

H
|
H

C
|
C

D

T

H

Y

A
x
P

P

G

C
|
C

V

L

D

A

V

A

C
|
C

P

P

T
x
R

G

K

A

A

S
x
I

F

Y

R

K

D

R

A

K

A

E

S

D

G

G

I

I

V

V

D

L

V

I

N

D

P

Q

D

K

L

R

C
|
C

V
x
R

G
|
G

C
x
Y

Q
x
R

Y
x
K

C
|
C

I

V

A

E

A

Q

C
|
C

P

P

Y

Y

R

K

V

K

R
x
P

F

M

I

Y

H

R

P

G

V

L

T

T

K

R

T

V

A
x
S

D

E

K
|
K

C
|
C

D

I

F
x
A

C
|
C

R

Y

-

P

-

R

-

I

-

E

-

G

K

R

T

D

N

S

L

L

Q

T

A

D

G

G

K

R

-

P

-

M

L

E

P
x
T

A
x
R

C
|
C

V

M

E

S

A

A

C
|
C

P
x
V

T

G

K

Q

A
x
I

L
x
R

T

L

F

Q

G

G

N

F

L

L

D

D

-

N

P

P

N

K

S

N

E

P

I

I

S

T

Q

W

L

L

L

I

R

R

-

H

Q

Q

K

K

P

I

T

A

Y

L

R

P

Y

L

K

Y

L

P

A

Q

L

F

G

G

T

T

K

E

P

P

K

N

L

I

Y

Y

R

Y

V

I

P

P

F

P

K

R

Y

W

G

A

binding fe3-s4 cluster: C186 (= C104), I191 (≠ S109), C207 (= C125), R208 (≠ V126), G209 (= G127), Y210 (≠ C128), R211 (≠ Q129), K212 (≠ Y130), C213 (= C131), S231 (≠ A149)
binding protoporphyrin ix containing fe: R188 (≠ T106), R208 (≠ V126), Y210 (≠ C128)
binding iron/sulfur cluster: Q171 (≠ R89), I173 (≠ S91), C174 (= C92), N175 (≠ Q93), H176 (= H94), C177 (= C95), P180 (≠ A98), C182 (= C100), C217 (= C135), P222 (≠ R140), K233 (= K151), C234 (= C152), A236 (≠ F154), C237 (= C155), T255 (≠ P166), R256 (≠ A167), C257 (= C168), C261 (= C172), V262 (≠ P173), I265 (≠ A176), R266 (≠ L177)

Sites not aligning to the query:

binding iron/sulfur cluster: 34, 35, 36, 37, 38, 40, 44, 48, 59, 60, 62

4v4cB Pyrogallol hydroxytransferase small subunit (see paper)
29% identity, 79% coverage: 36:212/223 of query aligns to 3:187/274 of 4v4cB

query
sites
4v4cB

R

Q

Y

Y

G

Y

M

M

V

V

H

I

D

D

E

V

S

A

L

K

C
|
C

I
x
Q

G
x
D

C
|
C

T
x
N

A
x
N

C
|
C

M

F

D

M

A

G

C
|
C

-

M

-

D

-

E

R

H

E

E

V

L

N

N

K

E

V

W

P

P

-

G

-

Y

-

T

-

A

-

S

-

M

-

Q

E

R

G

G

V

H

S

R

R

W

L

M

T

N

I

I

I

E

R

R

S

R

E

E

P

-

Q

R

G

G

E

T

F

Y

P

P

-

R

-

N

D

D

V
x
I

K
x
N

Y

Y

R

R

F

-

R

P

K

T

S

P

C
|
C

Q
x
M

H
|
H

C
|
C

D

E

H

N

A

A

P

P

C
|
C

V

V

D

-

V

A

C

K

P

G

T

N

G

G

A

A

S

V

F

Y

R

Q

D

-

A

R

A

E

S

D

G

G

I

I

V
|
V

D

L

V

I

N

D

P

P

D

E

L

K

C

A

V

K

G

G

C

K

Q

K

Y

E

C

L

I

L

A

D

A

T

C
|
C

P

P

Y

Y

R

G

V
|
V

R

M

F

Y

I

W

H

N

P

E

V

E

T

E

K

N

T

V

A

A

D

Q

K

K

C
|
C

D

T

F
x
M

C
|
C

R

A

K

H

-

L

-

D

T

D

N

E

L

S

Q

W

A

A

G

P

K

K

L

M

P
|
P

A

R

C
|
C

V

A

E

H

A

N

C
|
C

P

G

T
x
S

K

F

A
x
V

L
x
Y

T

E

F

F

G

-

N

-

L

L

D

K

D

T

P

T

N

P

S

E

E

A

I

M

S

A

Q

K

L

K

L

V

R

E

Q

E

K

E

P

G

T

L

Y

E

R

V

Y

I

K

K

L

P

A

E

L

L

G

G

T

T

K

K

P

P

K

R

L

V

Y

Y

binding calcium ion: I61 (≠ V83), N62 (≠ K84)
binding iron/sulfur cluster: C13 (= C46), Q14 (≠ I47), D15 (≠ G48), C16 (= C49), N17 (≠ T50), N18 (≠ A51), C19 (= C52), C23 (= C56), C68 (= C92), M69 (≠ Q93), H70 (= H94), C71 (= C95), C76 (= C100), V92 (= V118), C109 (= C135), V113 (= V139), C126 (= C152), M128 (≠ F154), C129 (= C155), P143 (= P166), C145 (= C168), C149 (= C172), S151 (≠ T174), V153 (≠ A176), Y154 (≠ L177)

Query Sequence

>18100 FitnessBrowser__Keio:18100
MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDESLCIGCTACMDACREVN
KVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVDVCPTGASFRDAASGIVDV
NPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNLQAGKLPACVEACPTKALTFG
NLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKYGEVSQ

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SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory