SitesBLAST
Comparing 18292 FitnessBrowser__Keio:18292 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6dkhC The crystal structure of l-idonate 5-dehydrogenase from escherichia coli str. K-12 substr. Mg1655
100% identity, 100% coverage: 1:343/343 of query aligns to 4:346/346 of 6dkhC
1e3jA Ketose reductase (sorbitol dehydrogenase) from silverleaf whitefly (see paper)
33% identity, 89% coverage: 31:334/343 of query aligns to 29:339/348 of 1e3jA
- active site: C38 (= C40), G39 (= G41), S40 (= S42), H43 (= H45), H63 (= H65), E64 (= E66), C93 (= C93), C96 (= C96), C99 (= C99), C107 (= C107), T111 (≠ R111), P150 (= P154), G154 (≠ A158)
- binding phosphate ion: A174 (≠ V178), A196 (≠ D201), R197 (≠ V202), S198 (= S203), R201 (≠ S206)
- binding zinc ion: C38 (= C40), H63 (= H65), E64 (= E66), C93 (= C93), C96 (= C96), C99 (= C99), C107 (= C107)
Sites not aligning to the query:
3qe3A Sheep liver sorbitol dehydrogenase (see paper)
33% identity, 87% coverage: 28:327/343 of query aligns to 27:329/351 of 3qe3A
- active site: C39 (= C40), G40 (= G41), S41 (= S42), H44 (= H45), H64 (= H65), E65 (= E66), R94 (≠ C93), D97 (≠ C96), C100 (= C99), S108 (≠ C107), F112 (≠ R111), P151 (= P154), G155 (≠ A158)
- binding glycerol: Y45 (= Y46), F54 (= F55), T116 (≠ S115), R293 (= R291)
- binding zinc ion: C39 (= C40), H64 (= H65), E65 (= E66)
Sites not aligning to the query:
1pl6A Human sdh/nadh/inhibitor complex (see paper)
32% identity, 87% coverage: 28:327/343 of query aligns to 32:334/356 of 1pl6A
- active site: C44 (= C40), G45 (= G41), S46 (= S42), H49 (= H45), H69 (= H65), E70 (= E66), R99 (≠ C93), D102 (≠ C96), C105 (= C99), S113 (≠ C107), F117 (≠ R111), P156 (= P154), G160 (≠ A158)
- binding 4-[2-(hydroxymethyl)pyrimidin-4-yl]-n,n-dimethylpiperazine-1-sulfonamide: C44 (= C40), S46 (= S42), I56 (≠ V52), F59 (= F55), H69 (= H65), E155 (= E153), L274 (≠ M267), F297 (= F290)
- binding nicotinamide-adenine-dinucleotide: G181 (= G179), P182 (= P180), I183 (= I181), D203 (= D201), L204 (≠ V202), R208 (≠ S206), C249 (≠ V242), T250 (≠ S243), V272 (= V265), G273 (= G266), L274 (≠ M267), F297 (= F290), R298 (= R291)
- binding zinc ion: C44 (= C40), H69 (= H65)
Sites not aligning to the query:
Q00796 Sorbitol dehydrogenase; SDH; (R,R)-butanediol dehydrogenase; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Ribitol dehydrogenase; RDH; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.4; EC 1.1.1.14; EC 1.1.1.56; EC 1.1.1.9 from Homo sapiens (Human) (see 10 papers)
32% identity, 87% coverage: 28:327/343 of query aligns to 33:335/357 of Q00796
- C45 (= C40) binding
- H70 (= H65) binding
- E71 (= E66) binding
- R110 (≠ N103) to P: in SORDD; results in protein aggregation
- H135 (≠ V132) to R: in SORDD; results in protein aggregation
- A153 (= A150) to D: in SORDD; uncertain significance; results in protein aggregation; dbSNP:rs145813597
- I184 (= I181) binding
- D204 (= D201) binding
- R209 (≠ S206) binding
- Q239 (≠ Y235) to L: in dbSNP:rs1042079
- N269 (≠ V261) to T: in dbSNP:rs930337
- VGL 273:275 (≠ VGM 265:267) binding
- VFR 297:299 (≠ SFR 289:291) binding
- V322 (≠ L314) to I: in SORDD; uncertain significance; dbSNP:rs149975952
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
P07846 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Ovis aries (Sheep) (see paper)
33% identity, 87% coverage: 28:327/343 of query aligns to 31:332/354 of P07846
- C43 (= C40) binding
- Y49 (= Y46) binding
- H67 (= H65) binding
- E68 (= E66) binding
- E153 (= E153) binding
- R296 (= R291) binding
- Y297 (≠ F292) binding
P27867 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Rattus norvegicus (Rat) (see paper)
32% identity, 94% coverage: 7:330/343 of query aligns to 11:338/357 of P27867
7y9pA Xylitol dehydrogenase s96c/s99c/y102c mutant(thermostabilized form) from pichia stipitis (see paper)
31% identity, 86% coverage: 31:326/343 of query aligns to 31:339/357 of 7y9pA
5vm2A Crystal structure of eck1772, an oxidoreductase/dehydrogenase of unknown specificity involved in membrane biogenesis from escherichia coli
29% identity, 87% coverage: 31:327/343 of query aligns to 30:328/347 of 5vm2A
- active site: C39 (= C40), G40 (= G41), S41 (= S42), H44 (= H45), H65 (= H65), E66 (= E66), C95 (= C93), C98 (= C96), C101 (= C99), C109 (= C107), D113 (≠ R111), P153 (= P154), G157 (≠ A158)
- binding magnesium ion: H65 (= H65), E66 (= E66), E152 (= E153)
- binding zinc ion: C95 (= C93), C98 (= C96), C101 (= C99), C109 (= C107)
Sites not aligning to the query:
B6HI95 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum) (see paper)
29% identity, 91% coverage: 32:342/343 of query aligns to 46:370/385 of B6HI95
- DI 212:213 (≠ DV 201:202) mutation to SR: Alters cofactor specificity from NAD to NADP; when associated with T-358.
- S358 (≠ G331) mutation to T: Alters cofactor specificity from NAD to NADP; when associated with 212-SR-213.
3m6iA L-arabinitol 4-dehydrogenase (see paper)
28% identity, 90% coverage: 32:341/343 of query aligns to 41:355/358 of 3m6iA
- active site: C49 (= C40), G50 (= G41), S51 (= S42), H54 (= H45), H74 (= H65), E75 (= E66), C104 (= C93), C107 (= C96), C110 (= C99), C118 (= C107), D122 (≠ R111), P160 (= P154), A164 (= A158), K352 (= K338)
- binding nicotinamide-adenine-dinucleotide: C49 (= C40), V163 (= V157), G185 (= G179), P186 (= P180), I187 (= I181), D207 (= D201), R212 (≠ S206), C255 (≠ V242), T256 (≠ S243), I278 (≠ V265), G279 (= G266), V280 (≠ M267), R304 (= R291)
- binding zinc ion: C49 (= C40), H74 (= H65), C104 (= C93), C107 (= C96), C110 (= C99), C118 (= C107)
Q7SI09 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) (see 2 papers)
28% identity, 90% coverage: 32:341/343 of query aligns to 45:359/363 of Q7SI09
- C53 (= C40) binding
- F59 (≠ Y46) mutation F->A,S,Y: No effect.
- H78 (= H65) binding
- E79 (= E66) binding
- C108 (= C93) binding
- C111 (= C96) binding
- C114 (= C99) binding
- C122 (= C107) binding
- E163 (= E153) binding
- PI 190:191 (= PI 180:181) binding
- D211 (= D201) binding
- DI 211:212 (≠ DV 201:202) mutation to SR: Alters cofactor specificity from NAD to NADP; when associated with T-348.
- R216 (≠ S206) binding
- I282 (≠ V265) binding
- QYR 306:308 (≠ SFR 289:291) binding
- S348 (≠ G331) mutation to T: Alters cofactor specificity from NAD to NADP; when associated with 211-SR-212.
4ilkA Crystal structure of short chain alcohol dehydrogenase (rspb) from e. Coli cft073 (efi target efi-506413) complexed with cofactor nadh
26% identity, 99% coverage: 6:343/343 of query aligns to 5:337/337 of 4ilkA
- active site: C40 (= C40), G41 (= G41), S42 (= S42), H45 (= H45), H59 (= H65), E60 (= E66), C89 (= C93), C92 (= C96), C95 (= C99), C103 (= C107), A107 (≠ R111), P145 (= P154), A149 (= A158), K332 (= K338)
- binding manganese (ii) ion: C40 (= C40), H59 (= H65), E60 (= E66), E144 (= E153)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G168 (= G177), G170 (= G179), P171 (= P180), I172 (= I181), D193 (= D201), R194 (≠ V202), R198 (≠ S206), N213 (≠ P221), A235 (≠ V242), A236 (≠ S243), C237 (≠ G244), I241 (≠ S248), M258 (≠ V265), F260 (≠ M267)
- binding zinc ion: C89 (= C93), C92 (= C96), C95 (= C99), C103 (= C107)
A2QAC0 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Aspergillus niger (strain ATCC MYA-4892 / CBS 513.88 / FGSC A1513) (see 2 papers)
27% identity, 91% coverage: 32:342/343 of query aligns to 47:371/386 of A2QAC0
- M70 (≠ F55) mutation to F: Abolishes enzyme activity.
- DI 213:214 (≠ DV 201:202) mutation to SR: Alters cofactor specificity from NAD to NADP; when associated with T-359.
- Y318 (≠ F290) mutation to F: Increases affinity for D-sorbitol.
- A359 (≠ G331) mutation to T: Alters cofactor specificity from NAD to NADP; when associated with 213-SR-214.
Q96V44 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Hypocrea jecorina (Trichoderma reesei) (see paper)
27% identity, 90% coverage: 32:341/343 of query aligns to 58:373/377 of Q96V44
- DI 224:225 (≠ DV 201:202) mutation to SR: Alters cofactor specificity from NAD to NADP; when associated with T-362.
- A362 (≠ G331) mutation to T: Alters cofactor specificity from NAD to NADP; when associated with 224-SR-225.
4uejA Closed state of galactitol-1-phosphate 5-dehydrogenase from e. Coli in complex with glycerol. (see paper)
29% identity, 91% coverage: 32:342/343 of query aligns to 30:345/346 of 4uejA
- active site: C38 (= C40), G39 (= G41), S40 (= S42), P43 (vs. gap), H59 (= H65), E60 (= E66), C89 (= C93), C92 (= C96), C95 (= C99), C103 (= C107), D107 (≠ R111), P145 (= P154), G149 (≠ A158), K341 (= K338)
- binding glycerol: H59 (= H65), E144 (= E153)
- binding zinc ion: C89 (= C93), C92 (= C96), C95 (= C99), C103 (= C107)
4a2cA Crystal structure of galactitol-1-phosphate dehydrogenase from escherichia coli (see paper)
29% identity, 91% coverage: 32:342/343 of query aligns to 30:345/346 of 4a2cA
- active site: C38 (= C40), G39 (= G41), S40 (= S42), P43 (vs. gap), H59 (= H65), E60 (= E66), C89 (= C93), C92 (= C96), C95 (= C99), C103 (= C107), D107 (≠ R111), P145 (= P154), G149 (≠ A158), K341 (= K338)
- binding zinc ion: C89 (= C93), C92 (= C96), C95 (= C99), C103 (= C107)
P07913 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Escherichia coli (strain K12) (see paper)
27% identity, 92% coverage: 27:341/343 of query aligns to 25:338/341 of P07913
- C38 (= C40) mutation to D: Shows only 1% of wild-type catalytic activity. This mutant can be stimulated to the wild-type activity level after incubation with Zn(+).; mutation to S: Loss of catalytic activity. This mutant cannot be stimulated to the wild-type activity level after incubation with Zn(+).
Q5JI69 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) (see paper)
27% identity, 87% coverage: 31:329/343 of query aligns to 33:334/350 of Q5JI69
- L179 (≠ I181) binding
- E199 (≠ D201) binding
- R204 (≠ S206) binding
- LGL 266:268 (≠ VGM 265:267) binding
- IT 291:292 (vs. gap) binding
3gfbA L-threonine dehydrogenase (tktdh) from the hyperthermophilic archaeon thermococcus kodakaraensis (see paper)
27% identity, 87% coverage: 31:329/343 of query aligns to 31:332/347 of 3gfbA
- active site: C40 (= C40), G41 (= G41), T42 (≠ S42), H45 (= H45), H65 (= H65), E66 (= E66), C95 (= C93), C98 (= C96), C101 (= C99), C109 (= C107), K113 (≠ R111), P151 (= P154), A155 (= A158)
- binding nicotinamide-adenine-dinucleotide: G173 (= G177), G175 (= G179), P176 (= P180), L177 (≠ I181), S196 (≠ A200), E197 (≠ D201), P198 (≠ V202), R202 (≠ S206), F241 (≠ V242), S242 (= S243), A244 (≠ H245), L264 (≠ V265), G265 (= G266), L266 (≠ M267), I289 (vs. gap), T290 (vs. gap)
Sites not aligning to the query:
Query Sequence
>18292 FitnessBrowser__Keio:18292
MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDLHYYQEGKVGNFMIKAP
MVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEHNENQCTDMRFFGSAMYFP
HVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIHAAHQAGELQGKRVFISGVGP
IGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVLVNPQNDDMDHWKAEKGYFDVSF
EVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPMMTLIGKEISLRGSFRFTSEFNTAV
SWLANGVINPLPLLSAEYPFTDLEEALRFAGDKTQAAKVQLVF
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory