SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 18311 FitnessBrowser__Keio:18311 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
43% identity, 99% coverage: 2:254/255 of query aligns to 11:264/265 of P07821

query
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P07821

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K50 (= K41) mutation to Q: Lack of activity.
D172 (= D163) mutation to E: Lack of activity.
E173 (= E164) mutation to A: Lack of activity.

5x40A Structure of a cbio dimer bound with amppcp (see paper)
38% identity, 88% coverage: 3:227/255 of query aligns to 5:229/280 of 5x40A

query
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5x40A

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binding phosphomethylphosphonic acid adenylate ester: F14 (≠ Y12), V18 (≠ D15), A20 (≠ V17), N40 (= N37), G41 (= G38), G43 (= G40), K44 (= K41), S45 (= S42), T46 (= T43), Q88 (= Q83), H139 (≠ T137), M140 (≠ E138), L141 (= L139), S142 (= S140), G144 (= G142), Q145 (= Q143), Q166 (≠ E164), H198 (= H196)
binding magnesium ion: S45 (= S42), Q88 (= Q83)

6mjpA Lptb(e163q)fgc from vibrio cholerae (see paper)
30% identity, 91% coverage: 3:235/255 of query aligns to 3:233/240 of 6mjpA

query
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6mjpA

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binding calcium ion: E111 (vs. gap), D115 (vs. gap)

6b8bA E. Coli lptb in complex with adp and a novobiocin derivative (see paper)
32% identity, 92% coverage: 2:235/255 of query aligns to 2:233/233 of 6b8bA

query
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6b8bA

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binding adenosine-5'-diphosphate: Y12 (= Y12), R15 (≠ D15), V17 (= V17), G38 (= G38), G40 (= G40), K41 (= K41), T42 (≠ S42), T43 (= T43)
binding (3s,5s,7s)-N-{7-[(3-O-carbamoyl-6-deoxy-5-methyl-4-O-methyl-beta-D-gulopyranosyl)oxy]-4-hydroxy-8-methyl-2-oxo-2H-1-benzopyran-3-yl}tricyclo[3.3.1.1~3,7~]decane-1-carboxamide: F89 (≠ V93), R90 (≠ Q94), R91 (≠ E95)

6s8nB Cryo-em structure of lptb2fgc in complex with lipopolysaccharide (see paper)
32% identity, 92% coverage: 2:235/255 of query aligns to 2:233/238 of 6s8nB

query
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6s8nB

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F

Y

binding 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione: R90 (≠ Q94), M133 (≠ R135), G134 (≠ L136), Q135 (≠ T137)

6s8gA Cryo-em structure of lptb2fgc in complex with amp-pnp (see paper)
32% identity, 92% coverage: 2:235/255 of query aligns to 2:233/238 of 6s8gA

query
sites
6s8gA

T

T

L

L

R

T

T

A

E

K

N

N

L

L

T

A

V

K

S

A

Y
|
Y

G

K

T

G

D
x
R

K

R

V

V

L

V

N

E

D

D

V

V

S

S

L

L

S

T

L

V

P

N

T

S

G

G

K

E

I

I

T

V

A

G

L

L

I

L

G

G

P

P

N
|
N

G

G

C

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

F

N

Y

C

M

F

V

S

V

R

G

L

I

L

V

M

P

P

R

Q

D

S

A

G

G

T

N

V

I

F

I

L

I

G

D

D

D

N

D

P

D

I

I

N

S

M

L

L

L

S

P

S

L

R

H

Q

A

L

R

A

A

R

R

-

G

L

I

S

G

L

Y

L

L

P

P

Q
|
Q

H

-

L

-

T

-

P

-

E

E

G

A

I

S

T

I

V

F

Q

R

E

R

L

L

V

S

S

V

Y

Y

G

D

R

N

-

L

N

M

P

A

W

V

L

L

S

Q

L

I

W

R

G

D

R

D

L

L

S

S

A

A

E

E

D

Q

N

R

A

E

-

D

R

R

V

A

N

N

V

E

A

L

M

M

N

E

Q

E

T

F

R

H

I

I

N

E

H

H

L

L

A

R

V

D

R

S

R

M

L

G

T

Q

E
x
S

L

L

S
|
S

G

G

Q
x
E

R

R

Q

R

R

R

A

V

F

E

L

I

A

A

M

R

V

A

L

L

A

A

Q

A

N

N

T

P

P

K

V

F

V

I

L

L

D

D

E

E

P

P

T

F

T

A

Y

G

L

V

D

D

I

P

N

I

H

S

Q

V

V

I

D

D

L

I

M

K

R

R

L

I

M

I

G

E

E

H

L

L

R

R

T

D

Q

S

G

G

K

L

T

G

V

V

V

L

A

I

V

T

L

D

H

H

D

N

L

V

N

R

Q

E

A

T

S

L

R

A

Y

V

C

C

D

E

Q

R

L

A

V

Y

V

I

M

V

A

S

N

Q

G

G

H

H

V

L

M

I

A

A

Q

H

G

G

T

T

P

P

E

T

E

E

V

I

M

L

T

Q

P

D

G

E

L

H

L

V

R

K

T

R

V

V

F

Y

binding phosphoaminophosphonic acid-adenylate ester: Y12 (= Y12), R15 (≠ D15), N37 (= N37), G40 (= G40), K41 (= K41), T42 (≠ S42), T43 (= T43), Q84 (= Q83), S136 (≠ E138), S138 (= S140), E141 (≠ Q143)

6b89A E. Coli lptb in complex with adp and novobiocin (see paper)
32% identity, 92% coverage: 2:235/255 of query aligns to 2:233/234 of 6b89A

query
sites
6b89A

T

T

L

L

R

T

T

A

E

K

N

N

L

L

T

A

V

K

S

A

Y
|
Y

G

K

T

G

D
x
R

K

R

V
|
V

L

V

N

E

D

D

V

V

S

S

L

L

S

T

L

V

P

N

T

S

G

G

K

E

I

I

T

V

A

G

L

L

I

L

G

G

P

P

N
|
N

G
|
G

C

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

F

N

Y

C

M

F

V

S

V

R

G

L

I

L

V

M

P

P

R

Q

D

S

A

G

G

T

N

V

I

F

I

L

I

G

D

D

D

N

D

P

D

I

I

N

S

M

L

L

L

S

P

S
x
L

R
x
H

Q

A

L

R

A

A

R

R

-

G

L

I

S

G

L

Y

L

L

P
|
P

Q
|
Q

H

-

L

-

T

-

P

-

E

E

G
x
A

I
x
S

T

I

V
x
F

Q
x
R

E
x
R

L
|
L

V

S

S

V

Y

Y

G

D

R

N

-

L

N

M

P

A

W
x
V

L

L

S

Q

L

I

W

R

G

D

R

D

L

L

S

S

A

A

E

E

D

Q

N

R

A

E

-

D

R

R

V

A

N

N

V

E

A

L

M

M

N

E

Q

E

T

F

R

H

I

I

N

E

H

H

L

L

A

R

V

D

R

S

R

M

L

G

T
x
Q

E

S

L

L

S

S

G

G

Q

E

R

R

Q

R

R

R

A

V

F

E

L

I

A

A

M
x
R

V

A

L

L

A

A

Q

A

N

N

T

P

P

K

V

F

V

I

L

L

D

D

E

E

P

P

T

F

T

A

Y

G

L

V

D

D

I

P

N

I

H

S

Q

V

V

I

D

D

L

I

M

K

R

R

L

I

M

I

G

E

E

H

L

L

R

R

T

D

Q

S

G

G

K

L

T

G

V

V

V

L

A

I

V

T

L

D

H

H

D

N

L

V

N

R

Q

E

A

T

S

L

R

A

Y

V

C

C

D

E

Q

R

L

A

V

Y

V

I

M

V

A

S

N

Q

G

G

H

H

V

L

M

I

A

A

Q

H

G

G

T

T

P

P

E

T

E

E

V

I

M

L

T

Q

P

D

G

E

L

H

L

V

R

K

T

R

V

V

F

Y

binding adenosine-5'-diphosphate: Y12 (= Y12), R15 (≠ D15), V17 (= V17), N37 (= N37), G38 (= G38), G40 (= G40), K41 (= K41), T42 (≠ S42), T43 (= T43)
binding magnesium ion: T42 (≠ S42), Q84 (= Q83)
binding novobiocin: L71 (≠ S71), H72 (≠ R72), P83 (= P82), A86 (≠ G90), S87 (≠ I91), F89 (≠ V93), R90 (≠ Q94), R91 (≠ E95), L92 (= L96), V101 (≠ W104), Q135 (≠ T137), R149 (≠ M151)

4p31A Crystal structure of a selenomethionine derivative of e. Coli lptb in complex with adp-magensium (see paper)
32% identity, 92% coverage: 2:235/255 of query aligns to 2:233/234 of 4p31A

query
sites
4p31A

T

T

L

L

R

T

T

A

E

K

N

N

L

L

T

A

V

K

S

A

Y
|
Y

G

K

T

G

D
x
R

K

R

V
|
V

L

V

N

E

D

D

V

V

S

S

L

L

S

T

L

V

P

N

T

S

G

G

K

E

I

I

T

V

A

G

L

L

I

L

G

G

P

P

N

N

G
|
G

C

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

F

N

Y

C

M

F

V

S

V

R

G

L

I

L

V

M

P

P

R

Q

D

S

A

G

G

T

N

V

I

F

I

L

I

G

D

D

D

N

D

P

D

I

I

N

S

M

L

L

L

S

P

S

L

R

H

Q

A

L

R

A

A

R

R

-

G

L

I

S

G

L

Y

L

L

P

P

Q
|
Q

H

-

L

-

T

-

P

-

E

E

G

A

I

S

T

I

V

F

Q

R

E

R

L

L

V

S

S

V

Y

Y

G

D

R

N

-

L

N

M

P

A

W

V

L

L

S

Q

L

I

W

R

G

D

R

D

L

L

S

S

A

A

E

E

D

Q

N

R

A

E

-

D

R

R

V

A

N

N

V

E

A

L

M

M

N

E

Q

E

T

F

R

H

I

I

N

E

H

H

L

L

A

R

V

D

R

S

R

M

L

G

T

Q

E

S

L

L

S

S

G

G

Q

E

R

R

Q

R

R

R

A

V

F

E

L

I

A

A

M

R

V

A

L

L

A

A

Q

A

N

N

T

P

P

K

V

F

V

I

L

L

D

D

E

E

P

P

T

F

T

A

Y

G

L

V

D

D

I

P

N

I

H

S

Q

V

V

I

D

D

L

I

M

K

R

R

L

I

M

I

G

E

E

H

L

L

R

R

T

D

Q

S

G

G

K

L

T

G

V

V

V

L

A

I

V

T

L

D

H

H

D

N

L

V

N

R

Q

E

A

T

S

L

R

A

Y

V

C

C

D

E

Q

R

L

A

V

Y

V

I

M

V

A

S

N

Q

G

G

H

H

V

L

M

I

A

A

Q

H

G

G

T

T

P

P

E

T

E

E

V

I

M

L

T

Q

P

D

G

E

L

H

L

V

R

K

T

R

V

V

F

Y

binding adenosine-5'-diphosphate: Y12 (= Y12), R15 (≠ D15), V17 (= V17), G38 (= G38), G40 (= G40), K41 (= K41), T42 (≠ S42), T43 (= T43)
binding magnesium ion: T42 (≠ S42), Q84 (= Q83)

7mdyC Lolcde nucleotide-bound
38% identity, 85% coverage: 3:219/255 of query aligns to 3:223/226 of 7mdyC

query
sites
7mdyC

L

L

R

Q

T

C

E

D

N

N

L

L

T

C

V

K

S

R

Y
|
Y

G

Q

-

E

-

G

-

S

-

V

-

Q

T

T

D

D

K

-

V

V

L

L

N

H

D

N

V

V

S

S

L

F

S

S

L

V

P

G

T

E

G

G

K

E

I

M

T

M

A

A

L

I

I

V

G

G

P

S

N

S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

N

H

C

L

F

L

S

G

R

G

L

L

L

D

M

T

P

P

Q

T

S

S

G

G

T

D

V

V

F

I

L

F

G

N

D

G

N

Q

P

P

I

M

N

S

M

K

L

L

S

S

R

A

Q

A

L

K

A

A

-

E

-

L

-

R

-

N

R

Q

R

K

L

L

S

G

L

F

L

I

P

Y

Q
|
Q

-

F

H

H

L

L

T

L

P

P

E

D

G

-

I

F

T

T

V

A

Q

L

E

E

L

N

V

V

S

A

Y

M

G

-

R

-

N

-

P

P

W

L

L

L

S

-

L

I

W

G

G

K

R

K

L

K

S

P

A

A

E

E

D

I

N

N

A

S

R

R

V

A

N

L

V

E

A

M

M

L

N

K

Q

A

T

V

R

G

I

L

N

D

H

H

L

R

A

A

V

N

R
x
H

R

R

L

P

T

S

E
|
E

L

L

S
|
S

G
|
G

Q

E

R

R

Q

Q

R

R

A

V

F

A

L

I

A

A

M

R

V

A

L

L

A

V

Q

N

N

N

T

P

P

R

V

L

V

V

L

L

L

A

D

D

E
|
E

P

P

T

T

T

G

Y
x
N

L

L

D

D

I

A

N

R

H

N

Q

A

V

D

D

S

L

I

M

F

R

Q

L

L

M

L

G

G

E

E

L

L

-

N

R

R

T

L

Q

Q

G

G

K

T

T

A

V

F

V

L

A

V

V

V

L

T

H
|
H

D

D

L

L

N

Q

Q

L

A

A

S

K

R

R

Y

M

C

S

D

R

Q

Q

L

L

V

-

V

E

M

M

A

R

N

D

G

G

H

R

V

L

M

T

A

A

Q

E

binding adp orthovanadate: Y12 (= Y12), G42 (= G38), S43 (≠ C39), G44 (= G40), K45 (= K41), S46 (= S42), T47 (= T43), Q91 (= Q83), H138 (≠ R134), E142 (= E138), S144 (= S140), G145 (= G141), G146 (= G142), E168 (= E164), N172 (≠ Y168), H201 (= H196)
binding magnesium ion: S46 (= S42), Q91 (= Q83)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
38% identity, 85% coverage: 3:219/255 of query aligns to 6:226/233 of P75957

query
sites
P75957

L

L

R

Q

T

C

E

D

N

N

L

L

T

C

V

K

S

R

Y

Y

G

Q

-

E

-

G

-

S

-

V

-

Q

T

T

D

D

K

-

V

V

L

L

N

H

D

N

V

V

S

S

L

F

S

S

L

V

P

G

T

E

G

G

K

E

I

M

T

M

A

A

L

I

I

V

G
|
G

P

S

N

S

G

G

C

S

G

G

K

K

S

S

T

T

L

L

N

H

C

L

F

L

S

G

R

G

L

L

L

D

M

T

P

P

Q

T

S

S

G

G

T

D

V

V

F

I

L

F

G

N

D

G

N

Q

P

P

I

M

N

S

M

K

L

L

S

S

R

A

Q

A

L

K

A

A

-

E

-

L

-

R

-

N

R

Q

R

K

L

L

S

G

L

F

L

I

P

Y

Q

Q

-

F

H

H

L

L

T

L

P

P

E

D

G

-

I

F

T

T

V

A

Q

L

E

E

L

N

V

V

S

A

Y

M

G

-

R

-

N

-

P

P

W

L

L

L

S

-

L

I

W

G

G

K

R

K

L

K

S

P

A

A

E

E

D

I

N

N

A

S

R

R

V

A

N

L

V

E

A

M

M

L

N

K

Q

A

T

V

R

G

I

L

N

D

H

H

L

R

A

A

V

N

R

H

R

R

L

P

T

S

E

E

L

L

S

S

G

G

Q

E

R

R

Q

Q

R

R

A

V

F

A

L

I

A

A

M

R

V

A

L

L

A

V

Q

N

N

N

T

P

P

R

V

L

V

V

L

L

L

A

D

D

E

E

P

P

T

T

T

G

Y

N

L

L

D

D

I

A

N

R

H

N

Q

A

V

D

D

S

L

I

M

F

R

Q

L

L

M

L

G

G

E

E

L

L

-

N

R

R

T

L

Q

Q

G

G

K

T

T

A

V

F

V

L

A

V

V

V

L

T

H

H

D

D

L

L

N

Q

Q

L

A

A

S

K

R

R

Y

M

C

S

D

R

Q

Q

L

L

V

-

V

E

M

M

A

R

N

D

G

G

H

R

V

L

M

T

A

A

Q

E

G42 (= G35) mutation to D: Loss of lipoprotein release when overexpressed.

6mhzA Vanadate trapped cryo-em structure of e.Coli lptb2fg transporter (see paper)
32% identity, 92% coverage: 2:235/255 of query aligns to 2:233/235 of 6mhzA

query
sites
6mhzA

T

T

L

L

R

T

T

A

E

K

N

N

L

L

T

A

V

K

S

A

Y
|
Y

G

K

T

G

D

R

K

R

V

V

L

V

N

E

D

D

V

V

S

S

L

L

S

T

L

V

P

N

T

S

G

G

K

E

I

I

T

V

A

G

L

L

I

L

G

G

P

P

N
|
N

G
|
G

C

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

F

N

Y

C

M

F

V

S

V

R

G

L

I

L

V

M

P

P

R

Q

D

S

A

G

G

T

N

V

I

F

I

L

I

G

D

D

D

N

D

P

D

I

I

N

S

M

L

L

L

S

P

S

L

R

H

Q

A

L

R

A

A

R

R

-

G

L

I

S

G

L

Y

L

L

P

P

Q
|
Q

H

-

L

-

T

-

P

-

E

E

G

A

I

S

T

I

V

F

Q

R

E

R

L

L

V

S

S

V

Y

Y

G

D

R

N

-

L

N

M

P

A

W

V

L

L

S

Q

L

I

W

R

G

D

R

D

L

L

S

S

A

A

E

E

D

Q

N

R

A

E

-

D

R

R

V

A

N

N

V

E

A

L

M

M

N

E

Q

E

T

F

R

H

I

I

N

E

H

H

L

L

A

R

V

D

R

S

R

M

L

G

T

Q

E
x
S

L

L

S
|
S

G
|
G

Q

E

R

R

Q

R

R

R

A

V

F

E

L

I

A

A

M

R

V

A

L

L

A

A

Q

A

N

N

T

P

P

K

V

F

V

I

L

L

D

D

E
|
E

P

P

T

F

T

A

Y
x
G

L

V

D

D

I

P

N

I

H

S

Q

V

V

I

D

D

L

I

M

K

R

R

L

I

M

I

G

E

E

H

L

L

R

R

T

D

Q

S

G

G

K

L

T

G

V

V

V

L

A

I

V

T

L

D

H
|
H

D

N

L

V

N

R

Q

E

A

T

S

L

R

A

Y

V

C

C

D

E

Q

R

L

A

V

Y

V

I

M

V

A

S

N

Q

G

G

H

H

V

L

M

I

A

A

Q

H

G

G

T

T

P

P

E

T

E

E

V

I

M

L

T

Q

P

D

G

E

L

H

L

V

R

K

T

R

V

V

F

Y

binding adp orthovanadate: Y12 (= Y12), N37 (= N37), G38 (= G38), G40 (= G40), K41 (= K41), T42 (≠ S42), T43 (= T43), Q84 (= Q83), S136 (≠ E138), S138 (= S140), G139 (= G141), G140 (= G142), E162 (= E164), G166 (≠ Y168), H194 (= H196)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
31% identity, 85% coverage: 11:226/255 of query aligns to 10:224/240 of 4ymuJ

query
sites
4ymuJ

S

N

Y
x
F

G

G

T

S

D

L

K

E

V
|
V

L

L

N

K

D

G

V

V

S

T

L

L

S

K

L

V

P

N

T

K

G

G

K

E

I

V

T

V

A

V

L

I

I

I

G

G

P

P

N
x
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

N

R

C

C

F

I

S

N

R

L

L

L

L

E

M

E

P

P

Q

T

S

K

G

G

T

E

V

V

F

F

L

I

G

D

D

G

N

V

P

K

I

I

N

N

-

N

-

G

M

K

L

V

S

N

S

I

R

N

Q

K

L

V

A

R

R

Q

R

K

L

V

S

G

L

M

L

V

P

F

Q

Q

H

H

H

-

L

-

T

-

P

-

E

-

G

-

I

-

T

-

V

-

Q

-

E

-

L

-

V

-

S

-

Y

-

G

-

R

-

N

-

P

-

W

-

L

F

S

N

L

L

W

F

G

P

R

H

L

L

S

T

A

A

E

I

D

E

N

N

-

I

-

T

-

L

A

A

R

P

V

V

N

K

V

V

A

K

-

K

M

M

N

N

Q

K

T

K

R

E

I

A

N

E

H

E

L

L

A

A

V

V

R

D

R

L

L

L

T

A

E

K

-

V

-

G

-

L

-

D

-

K

-

D

-

Q

-

Y

-

P

-

I

-

K

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

A

L

F

A

L

I

A

A

M

R

V

A

L

L

A

A

Q

M

N

Q

T

P

P

E

V

V

V

M

L

L

L

F

D

D

E
|
E

P

P

T

T

T

S

Y

A

L

L

D

D

I

P

N

E

H

M

Q

V

V

K

D

E

L

V

M

L

R

N

L

V

M

M

G

K

E

Q

L

L

R

A

T

N

Q

E

G

G

K

M

T

T

V

M

V

V

A

V

V

V

L

T

H
|
H

D

E

L

M

N

G

Q

F

A

A

S

R

R

E

Y

V

C

G

D

D

Q

R

L

V

V

I

V

F

M

M

A

D

N

D

G

G

H

V

V

I

M

V

A

E

Q

E

G

G

T

T

P

P

E

E

V

I

M

F

binding adenosine-5'-triphosphate: F11 (≠ Y12), V16 (= V17), S36 (≠ N37), G37 (= G38), S38 (≠ C39), G39 (= G40), K40 (= K41), S41 (= S42), T42 (= T43), E162 (= E164), H194 (= H196)
binding magnesium ion: S41 (= S42), E162 (= E164)

7arlD Lolcde in complex with lipoprotein and adp (see paper)
38% identity, 85% coverage: 3:218/255 of query aligns to 3:222/222 of 7arlD

query
sites
7arlD

L

L

R

Q

T

C

E

D

N

N

L

L

T

C

V

K

S

R

Y

Y

G

Q

-

E

-

G

-

S

-

V

-

Q

T

T

D

D

K

-

V

V

L

L

N

H

D

N

V

V

S

S

L

F

S

S

L

V

P

G

T

E

G

G

K

E

I

M

T

M

A

A

L

I

I

V

G

G

P

S

N

S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T

T

L

L

N

H

C

L

F

L

S

G

R

G

L

L

L

D

M

T

P

P

Q

T

S

S

G

G

T

D

V

V

F

I

L

F

G

N

D

G

N

Q

P

P

I

M

N

S

M

K

L

L

S

S

R

A

Q

A

L

K

A

A

-

E

-

L

-

R

-

N

R

Q

R

K

L

L

S

G

L

F

L

I

P

Y

Q

Q

-

F

H

H

L

L

T

L

P

P

E

D

G

-

I

F

T

T

V

A

Q

L

E

E

L

N

V

V

S

A

Y

M

G

-

R

-

N

-

P

P

W

L

L

L

S

-

L

I

W

G

G

K

R

K

L

K

S

P

A

A

E

E

D

I

N

N

A

S

R

R

V

A

N

L

V

E

A

M

M

L

N

K

Q

A

T

V

R

G

I

L

N

D

H

H

L

R

A

A

V

N

R

H

R

R

L

P

T

S

E

E

L

L

S

S

G

G

Q

E

R

R

Q

Q

R

R

A

V

F

A

L

I

A

A

M

R

V

A

L

L

A

V

Q

N

N

N

T

P

P

R

V

L

V

V

L

L

L

A

D

D

E

E

P

P

T

T

T

G

Y

N

L

L

D

D

I

A

N

R

H

N

Q

A

V

D

D

S

L

I

M

F

R

Q

L

L

M

L

G

G

E

E

L

L

-

N

R

R

T

L

Q

Q

G

G

K

T

T

A

V

F

V

L

A

V

V

V

L

T

H

H

D

D

L

L

N

Q

Q

L

A

A

S

K

R

R

Y

M

C

S

D

R

Q

Q

L

L

V

-

V

E

M

M

A

R

N

D

G

G

H

R

V

L

M

T

A

A

binding adenosine-5'-diphosphate: G42 (= G38), G44 (= G40), K45 (= K41), S46 (= S42)

3fvqB Crystal structure of the nucleotide binding domain fbpc complexed with atp (see paper)
37% identity, 88% coverage: 2:225/255 of query aligns to 3:225/350 of 3fvqB

query
sites
3fvqB

T

A

L

L

R

H

T

I

E

G

N

H

L

L

T

S

V

K

S

S

Y
x
F

G
x
Q

T

N

D
x
T

K

P

V
|
V

L

L

N

N

D

D

V

I

S

S

L

L

S

S

L

L

P

D

T

P

G

G

K

E

I

I

T

L

A

F

L

I

I

I

G

G

P

A

N
x
S

G
|
G

C
|
C

G
|
G

K
|
K

S
x
T

T
|
T

L

L

N

R

C

C

F

L

S

A

R

G

L

F

L

E

M

Q

P

P

Q

D

S

S

G

G

T

E

V

I

F

S

L

L

G

S

D

G

N

K

P

T

I

I

-

F

-

S

-

K

-

N

N

T

M

N

L

L

S

P

S

V

R

R

Q

E

L

-

A

-

R

R

L

L

S

G

L

Y

L

L

P

V

Q
|
Q

H

E

H

G

L

V

T

L

P

F

E

P

G

H

I

L

T

T

V

V

Q

Y

E

R

L

N

V

I

S

A

Y

Y

G

G

R

L

N

G

P

-

W

-

L

-

S

N

L

G

W

K

G

G

R

R

L

-

S

T

A

A

E

Q

D

E

N

R

A

Q

R

R

V

I

N

E

V

A

A

M

M

L

N

E

Q

L

T

T

R

G

I

I

N

S

H

E

L

L

A

A

V

G

R
|
R

R

Y

L

P

T

H

E
|
E

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

Q

Q

Q

R

R

A

A

F

A

L

L

A

A

M

R

V

A

L

L

A

A

Q

P

N

D

T

P

P

E

V

L

V

I

L

L

D

D

E

E

P

P

T

F

T

S

Y

A

L

L

D

D

I

E

N

Q

H

L

Q

R

V

R

D

Q

L

I

M

R

R

E

-

D

L

M

M

I

G

A

E

A

L

L

R

R

T

A

Q

N

G

G

K

K

T

S

V

A

V

V

A

F

V

V

L

S

H

H

D

D

L

R

N

E

Q

E

A

A

S

L

R

Q

Y

Y

C

A

D

D

Q

R

L

I

V

A

V

V

M

M

A

K

N

Q

G

G

H

R

V

I

M

L

A

Q

Q

T

G

A

T

S

P

P

E

H

E

E

V

L

binding adenosine-5'-triphosphate: F13 (≠ Y12), Q14 (≠ G13), T16 (≠ D15), V18 (= V17), S38 (≠ N37), G39 (= G38), C40 (= C39), G41 (= G40), K42 (= K41), T43 (≠ S42), T44 (= T43), R133 (= R134), E137 (= E138), S139 (= S140), G141 (= G142), Q142 (= Q143)
binding calcium ion: T43 (≠ S42), Q86 (= Q83)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
32% identity, 88% coverage: 2:225/255 of query aligns to 3:221/371 of P68187

query
sites
P68187

T

S

L

V

R

Q

T

L

E

Q

N

N

L

V

T

T

V

K

S

A

Y

W

G

G

T

E

D

V

K

V

V

V

L

S

N

K

D

D

V

I

S

N

L

L

S

D

L

I

P

H

T

E

G

G

K

E

I

F

T

V

A

V

L

F

I

V

G

G

P

P

N

S

G

G

C

C

G

G

K

K

S

S

T

T

L

L

N

R

C

M

F

I

S

A

R

G

L

L

L

E

M

T

P

I

Q

T

S

S

G

G

T

D

V

L

F

F

L

I

G

G

D

E

N

K

P

R

I

M

N

N

M

-

L

-

S

D

S

T

R

P

Q

P

L

A

A

E

R

R

R

G

L

V

S

G

L

M

L

V

P

F

Q

Q

H

S

H

Y

L
x
A

T

L

P

Y

E

P

G

H

I

L

T

S

V

V

Q

A

E

E

L

N

V

M

S

S

Y

F

G

G

R

-

N

-

P

-

W

-

L

L

S

K

L

L

W

A

G

G

R

A

L
x
K

S

K

A

E

E

V

D

I

N

N

A

Q

R

R

V
|
V

N

N

V

Q

A
x
V

M

A

N
x
E

Q

V

T

L

R

Q

I

L

N
x
A

H

H

L

L

A

L

V

D

R

R

R

K

L

P

T

K

E

A

L

L

S

S

G

G

G
|
G

Q

Q

R

R

Q

Q

R

R

A

V

F

A

L

I

A

G

M

R

V

T

L

L

A

V

Q

A

N

E

T

P

P

S

V

V

V

F

L

L

D
|
D

E

E

P

P

T

L

T

S

Y

N

L

L

D

D

-

A

-

L

-

R

I

V

N

Q

H

M

Q

R

V

I

D

E

L

I

M

S

R

R

L

L

M

H

G

K

E

R

L

L

R

-

T

-

Q

-

G

G

K

R

T

T

V

M

V

I

A

Y

V

V

L

T

H

H

D

D

L

Q

N

V

Q

E

A

A

S

M

R

T

Y

L

C

A

D

D

Q

K

L

I

V

V

M

L

A

D

N

A

G

G

H

R

V

V

M

A

A

Q

Q

V

G

G

T

K

P

P

E

L

E

E

V

L

A85 (≠ L86) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ L111) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V119) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ A122) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ N124) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ N129) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G142) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D163) mutation to N: Loss of maltose transport but retains ability to repress mal genes.

Sites not aligning to the query:

228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
32% identity, 88% coverage: 2:225/255 of query aligns to 2:220/371 of 3puyA

query
sites
3puyA

T

S

L

V

R

Q

T

L

E

Q

N

N

L

V

T

T

V

K

S

A

Y
x
W

G

G

T

E

D

V

K

V

V

V

L

S

N

K

D

D

V

I

S

N

L

L

S

D

L

I

P

H

T

E

G

G

K

E

I

F

T

V

A

V

L

F

I

V

G

G

P

P

N
x
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

N

R

C

M

F

I

S

A

R

G

L

L

L

E

M

T

P

I

Q

T

S

S

G

G

T

D

V

L

F

F

L

I

G

G

D

E

N

K

P

R

I

M

N

N

M

-

L

-

S

D

S

T

R

P

Q

P

L

A

A

E

R

R

R

G

L

V

S

G

L

M

L

V

P

F

Q
|
Q

H

S

H

Y

L

A

T

L

P

Y

E

P

G

H

I

L

T

S

V

V

Q

A

E

E

L

N

V

M

S

S

Y

F

G

G

R

-

N

-

P

-

W

-

L

L

S

K

L

L

W

A

G

G

R

A

L

K

S

K

A

E

E

V

D

I

N

N

A

Q

R

R

V

V

N

N

V

Q

A

V

M

A

N

E

Q

V

T

L

R

Q

I

L

N

A

H

H

L

L

A

L

V

D

R
|
R

R

K

L

P

T

K

E
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

A

V

F

A

L

I

A

G

M

R

V

T

L

L

A

V

Q

A

N

E

T

P

P

S

V

V

V

F

L

L

D

D

E
|
E

P

P

T

L

T

S

Y

N

L

L

D

D

-

A

-

L

-

R

I

V

N

Q

H

M

Q

R

V

I

D

E

L

I

M

S

R

R

L

L

M

H

G

K

E

R

L

L

R

-

T

-

Q

-

G

G

K

R

T

T

V

M

V

I

A

Y

V

V

L

T

H
|
H

D

D

L

Q

N

V

Q

E

A

A

S

M

R

T

Y

L

C

A

D

D

Q

K

L

I

V

V

M

L

A

D

N

A

G

G

H

R

V

V

M

A

A

Q

Q

V

G

G

T

K

P

P

E

L

E

E

V

L

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y12), S37 (≠ N37), G38 (= G38), C39 (= C39), G40 (= G40), K41 (= K41), S42 (= S42), T43 (= T43), Q81 (= Q83), R128 (= R134), A132 (≠ E138), S134 (= S140), G136 (= G142), Q137 (= Q143), E158 (= E164), H191 (= H196)
binding magnesium ion: S42 (= S42), Q81 (= Q83)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
32% identity, 88% coverage: 2:225/255 of query aligns to 2:220/371 of 3puxA

query
sites
3puxA

T

S

L

V

R

Q

T

L

E

Q

N

N

L

V

T

T

V

K

S

A

Y
x
W

G

G

T

E

D

V

K

V

V

V

L

S

N

K

D

D

V

I

S

N

L

L

S

D

L

I

P

H

T

E

G

G

K

E

I

F

T

V

A

V

L

F

I

V

G

G

P

P

N
x
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

N

R

C

M

F

I

S

A

R

G

L

L

L

E

M

T

P

I

Q

T

S

S

G

G

T

D

V

L

F

F

L

I

G

G

D

E

N

K

P

R

I

M

N

N

M

-

L

-

S

D

S

T

R

P

Q

P

L

A

A

E

R

R

R

G

L

V

S

G

L

M

L

V

P

F

Q
|
Q

H

S

H

Y

L

A

T

L

P

Y

E

P

G

H

I

L

T

S

V

V

Q

A

E

E

L

N

V

M

S

S

Y

F

G

G

R

-

N

-

P

-

W

-

L

L

S

K

L

L

W

A

G

G

R

A

L

K

S

K

A

E

E

V

D

I

N

N

A

Q

R

R

V

V

N

N

V

Q

A

V

M

A

N

E

Q

V

T

L

R

Q

I

L

N

A

H

H

L

L

A

L

V

D

R
|
R

R

K

L

P

T

K

E

A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

A

V

F

A

L

I

A

G

M

R

V

T

L

L

A

V

Q

A

N

E

T

P

P

S

V

V

V

F

L

L

D

D

E

E

P

P

T

L

T

S

Y

N

L

L

D

D

-

A

-

L

-

R

I

V

N

Q

H

M

Q

R

V

I

D

E

L

I

M

S

R

R

L

L

M

H

G

K

E

R

L

L

R

-

T

-

Q

-

G

G

K

R

T

T

V

M

V

I

A

Y

V

V

L

T

H
|
H

D

D

L

Q

N

V

Q

E

A

A

S

M

R

T

Y

L

C

A

D

D

Q

K

L

I

V

V

M

L

A

D

N

A

G

G

H

R

V

V

M

A

A

Q

Q

V

G

G

T

K

P

P

E

L

E

E

V

L

binding adenosine-5'-diphosphate: W12 (≠ Y12), G38 (= G38), C39 (= C39), G40 (= G40), K41 (= K41), S42 (= S42), T43 (= T43), R128 (= R134), S134 (= S140), Q137 (= Q143)
binding beryllium trifluoride ion: S37 (≠ N37), G38 (= G38), K41 (= K41), Q81 (= Q83), S134 (= S140), G136 (= G142), H191 (= H196)
binding magnesium ion: S42 (= S42), Q81 (= Q83)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
32% identity, 88% coverage: 2:225/255 of query aligns to 2:220/371 of 3puwA

query
sites
3puwA

T

S

L

V

R

Q

T

L

E

Q

N

N

L

V

T

T

V

K

S

A

Y
x
W

G

G

T

E

D

V

K

V

V
|
V

L

S

N

K

D

D

V

I

S

N

L

L

S

D

L

I

P

H

T

E

G

G

K

E

I

F

T

V

A

V

L

F

I

V

G

G

P

P

N
x
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

N

R

C

M

F

I

S

A

R

G

L

L

L

E

M

T

P

I

Q

T

S

S

G

G

T

D

V

L

F

F

L

I

G

G

D

E

N

K

P

R

I

M

N

N

M

-

L

-

S

D

S

T

R

P

Q

P

L

A

A

E

R

R

R

G

L

V

S

G

L

M

L

V

P

F

Q
|
Q

H

S

H

Y

L

A

T

L

P

Y

E

P

G

H

I

L

T

S

V

V

Q

A

E

E

L

N

V

M

S

S

Y

F

G

G

R

-

N

-

P

-

W

-

L

L

S

K

L

L

W

A

G

G

R

A

L

K

S

K

A

E

E

V

D

I

N

N

A

Q

R

R

V

V

N

N

V

Q

A

V

M

A

N

E

Q

V

T

L

R

Q

I

L

N

A

H

H

L

L

A

L

V

D

R
|
R

R

K

L

P

T

K

E
x
A

L

L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

A

V

F

A

L

I

A

G

M

R

V

T

L

L

A

V

Q

A

N

E

T

P

P

S

V

V

V

F

L

L

D

D

E
|
E

P

P

T

L

T

S

Y

N

L

L

D

D

-

A

-

L

-

R

I

V

N

Q

H

M

Q

R

V

I

D

E

L

I

M

S

R

R

L

L

M

H

G

K

E

R

L

L

R

-

T

-

Q

-

G

G

K

R

T

T

V

M

V

I

A

Y

V

V

L

T

H
|
H

D

D

L

Q

N

V

Q

E

A

A

S

M

R

T

Y

L

C

A

D

D

Q

K

L

I

V

V

M

L

A

D

N

A

G

G

H

R

V

V

M

A

A

Q

Q

V

G

G

T

K

P

P

E

L

E

E

V

L

binding adenosine-5'-diphosphate: W12 (≠ Y12), V17 (= V17), G38 (= G38), C39 (= C39), G40 (= G40), K41 (= K41), S42 (= S42), T43 (= T43), R128 (= R134), A132 (≠ E138), S134 (= S140), Q137 (= Q143)
binding tetrafluoroaluminate ion: S37 (≠ N37), G38 (= G38), K41 (= K41), Q81 (= Q83), S134 (= S140), G135 (= G141), G136 (= G142), E158 (= E164), H191 (= H196)
binding magnesium ion: S42 (= S42), Q81 (= Q83)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
32% identity, 88% coverage: 2:225/255 of query aligns to 2:220/371 of 3puvA

query
sites
3puvA

T

S

L

V

R

Q

T

L

E

Q

N

N

L

V

T

T

V

K

S

A

Y
x
W

G

G

T

E

D

V

K

V

V
|
V

L

S

N

K

D

D

V

I

S

N

L

L

S

D

L

I

P

H

T

E

G

G

K

E

I

F

T

V

A

V

L

F

I

V

G

G

P

P

N

S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

N

R

C

M

F

I

S

A

R

G

L

L

L

E

M

T

P

I

Q

T

S

S

G

G

T

D

V

L

F

F

L

I

G

G

D

E

N

K

P

R

I

M

N

N

M

-

L

-

S

D

S

T

R

P

Q

P

L

A

A

E

R

R

R

G

L

V

S

G

L

M

L

V

P

F

Q
|
Q

H

S

H

Y

L

A

T

L

P

Y

E

P

G

H

I

L

T

S

V

V

Q

A

E

E

L

N

V

M

S

S

Y

F

G

G

R

-

N

-

P

-

W

-

L

L

S

K

L

L

W

A

G

G

R

A

L

K

S

K

A

E

E

V

D

I

N

N

A

Q

R

R

V

V

N

N

V

Q

A

V

M

A

N

E

Q

V

T

L

R

Q

I

L

N

A

H

H

L

L

A

L

V

D

R
|
R

R

K

L

P

T

K

E
x
A

L

L

S
|
S

G

G

Q
|
Q

R

R

Q

Q

R

R

A

V

F

A

L

I

A

G

M

R

V

T

L

L

A

V

Q

A

N

E

T

P

P

S

V

V

V

F

L

L

D

D

E

E

P

P

T

L

T

S

Y

N

L

L

D

D

-

A

-

L

-

R

I

V

N

Q

H

M

Q

R

V

I

D

E

L

I

M

S

R

R

L

L

M

H

G

K

E

R

L

L

R

-

T

-

Q

-

G

G

K

R

T

T

V

M

V

I

A

Y

V

V

L

T

H

H

D

D

L

Q

N

V

Q

E

A

A

S

M

R

T

Y

L

C

A

D

D

Q

K

L

I

V

V

M

L

A

D

N

A

G

G

H

R

V

V

M

A

A

Q

Q

V

G

G

T

K

P

P

E

L

E

E

V

L

binding adenosine-5'-diphosphate: W12 (≠ Y12), V17 (= V17), G38 (= G38), C39 (= C39), G40 (= G40), K41 (= K41), S42 (= S42), T43 (= T43), R128 (= R134), A132 (≠ E138), S134 (= S140), Q137 (= Q143)
binding magnesium ion: S42 (= S42), Q81 (= Q83)

6mbnA Lptb e163q in complex with atp (see paper)
32% identity, 92% coverage: 2:235/255 of query aligns to 3:234/241 of 6mbnA

query
sites
6mbnA

T

T

L

L

R

T

T

A

E

K

N

N

L

L

T

A

V

K

S

A

Y
|
Y

G

K

T

G

D
x
R

K

R

V
|
V

L

V

N

E

D

D

V

V

S

S

L

L

S

T

L

V

P

N

T

S

G

G

K

E

I

I

T

V

A

G

L

L

I

L

G

G

P

P

N
|
N

G
|
G

C

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

F

N

Y

C

M

F

V

S

V

R

G

L

I

L

V

M

P

P

R

Q

D

S

A

G

G

T

N

V

I

F

I

L

I

G

D

D

D

N

D

P

D

I

I

N

S

M

L

L

L

S

P

S

L

R

H

Q

A

L

R

A

A

R

R

-

G

L

I

S

G

L

Y

L

L

P

P

Q

Q

H

-

L

-

T

-

P

-

E

E

G

A

I

S

T

I

V

F

Q

R

E

R

L

L

V

S

S

V

Y

Y

G

D

R

N

-

L

N

M

P

A

W

V

L

L

S

Q

L

I

W

R

G

D

R

D

L

L

S

S

A

A

E

E

D

Q

N

R

A

E

-

D

R

R

V

A

N

N

V

E

A

L

M

M

N

E

Q

E

T

F

R

H

I

I

N

E

H

H

L

L

A

R

V

D

R

S

R

M

L

G

T

Q

E

S

L

L

S

S

G

G

Q

E

R

R

Q

R

R

R

A

V

F

E

L

I

A

A

M

R

V

A

L

L

A

A

Q

A

N

N

T

P

P

K

V

F

V

I

L

L

D

D

E

Q

P

P

T

F

T

A

Y

G

L

V

D

D

I

P

N

I

H

S

Q

V

V

I

D

D

L

I

M

K

R

R

L

I

M

I

G

E

E

H

L

L

R

R

T

D

Q

S

G

G

K

L

T

G

V

V

V

L

A

I

V

T

L

D

H
|
H

D

N

L

V

N

R

Q

E

A

T

S

L

R

A

Y

V

C

C

D

E

Q

R

L

A

V

Y

V

I

M

V

A

S

N

Q

G

G

H

H

V

L

M

I

A

A

Q

H

G

G

T

T

P

P

E

T

E

E

V

I

M

L

T

Q

P

D

G

E

L

H

L

V

R

K

T

R

V

V

F

Y

binding adenosine-5'-triphosphate: Y13 (= Y12), R16 (≠ D15), V18 (= V17), N38 (= N37), G39 (= G38), G41 (= G40), K42 (= K41), T43 (≠ S42), T44 (= T43), H195 (= H196)

Query Sequence

>18311 FitnessBrowser__Keio:18311
MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTLLNCFSRLLMPQSGTVF
LGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRNPWLSLWGRLSAEDNARVN
VAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVVLLDEPTTYLDINHQVDLMRL
MGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMAQGTPEEVMTPGLLRTVFSVEAE
IHPEPVSGRPMCLMR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory