SitesBLAST
Comparing 18382 FitnessBrowser__Keio:18382 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4ilkA Crystal structure of short chain alcohol dehydrogenase (rspb) from e. Coli cft073 (efi target efi-506413) complexed with cofactor nadh
32% identity, 100% coverage: 2:340/340 of query aligns to 2:337/337 of 4ilkA
- active site: C40 (= C40), G41 (= G41), S42 (≠ T42), H45 (= H45), H59 (= H62), E60 (= E63), C89 (= C92), C92 (= C95), C95 (= C98), C103 (= C106), A107 (≠ S110), P145 (= P147), A149 (≠ S151), K332 (= K335)
- binding manganese (ii) ion: C40 (= C40), H59 (= H62), E60 (= E63), E144 (= E146)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G168 (= G170), G170 (= G172), P171 (= P173), I172 (= I174), D193 (= D193), R194 (≠ T194), R198 (= R198), N213 (≠ Q221), A235 (= A237), A236 (≠ T238), C237 (≠ G239), I241 (≠ A243), M258 (≠ V260), F260 (≠ L262)
- binding zinc ion: C89 (= C92), C92 (= C95), C95 (= C98), C103 (= C106)
1e3jA Ketose reductase (sorbitol dehydrogenase) from silverleaf whitefly (see paper)
33% identity, 91% coverage: 14:324/340 of query aligns to 12:330/348 of 1e3jA
- active site: C38 (= C40), G39 (= G41), S40 (≠ T42), H43 (= H45), H63 (= H62), E64 (= E63), C93 (= C92), C96 (= C95), C99 (= C98), C107 (= C106), T111 (≠ S110), P150 (= P147), G154 (≠ S151)
- binding phosphate ion: A174 (= A171), A196 (≠ D193), R197 (≠ T194), S198 (= S195), R201 (= R198)
- binding zinc ion: C38 (= C40), H63 (= H62), E64 (= E63), C93 (= C92), C96 (= C95), C99 (= C98), C107 (= C106)
Sites not aligning to the query:
2dfvA Hyperthermophilic threonine dehydrogenase from pyrococcus horikoshii (see paper)
32% identity, 91% coverage: 14:324/340 of query aligns to 16:331/346 of 2dfvA
- active site: C40 (= C40), G41 (= G41), T42 (= T42), H45 (= H45), H65 (= H62), E66 (= E63), C95 (= C92), C98 (= C95), C101 (= C98), C109 (= C106), K113 (≠ S110), P151 (= P147), A155 (≠ S151)
- binding nicotinamide-adenine-dinucleotide: G175 (= G172), P176 (= P173), L177 (≠ I174), E197 (≠ D193), P198 (≠ T194), R202 (= R198), F241 (≠ A237), S242 (≠ T238), A244 (≠ N240), L264 (≠ V260), G265 (= G261), L266 (= L262), I289 (≠ G283), T290 (≠ S284)
- binding zinc ion: C95 (= C92), C101 (= C98), C109 (= C106)
Sites not aligning to the query:
O58389 L-threonine 3-dehydrogenase; L-ThrDH; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 2 papers)
32% identity, 91% coverage: 14:324/340 of query aligns to 18:333/348 of O58389
- C42 (= C40) binding
- T44 (= T42) mutation to A: Total loss of enzymatic activity.
- H67 (= H62) binding
- E68 (= E63) binding
- C97 (= C92) binding
- C100 (= C95) binding
- C103 (= C98) binding
- C111 (= C106) binding
- E152 (= E146) mutation E->A,Q: Almost complete loss of enzymatic activity.; mutation to C: 120-fold decrease in catalytic efficiency.; mutation to D: Shows 3-fold higher turnover rate and reduced affinities toward L-threonine and NAD(+), compared to wild-type.; mutation to K: Total loss of enzymatic activity.
- L179 (≠ I174) binding
- E199 (≠ D193) binding ; mutation to A: Large decrease in affinity for NAD(+).
- R204 (= R198) binding ; mutation to A: Large decrease in affinity for NAD(+).
- LGL 266:268 (≠ VGL 260:262) binding
- IT 291:292 (≠ GS 283:284) binding
- R294 (≠ N286) mutation to A: 4000-fold decrease in catalytic efficiency.
4ejmA Crystal structure of a putative zinc-binding dehydrogenase (target psi-012003) from sinorhizobium meliloti 1021 bound to NADP
33% identity, 97% coverage: 2:330/340 of query aligns to 2:329/342 of 4ejmA
- active site: C40 (= C40), G41 (= G41), T42 (= T42), H45 (= H45), H61 (= H62), E62 (= E63), C91 (= C92), C94 (= C95), C97 (= C98), C105 (= C106), R109 (≠ S110), P147 (= P147), C151 (≠ S151)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G170 (= G170), G172 (= G172), V173 (≠ P173), I174 (= I174), T194 (= T194), R195 (≠ S195), Q196 (≠ P196), K199 (≠ R199), C240 (≠ A237), E245 (≠ H242), T246 (≠ A243), L263 (≠ V260), V265 (≠ L262), I291 (≠ A287)
- binding zinc ion: C91 (= C92), C94 (= C95), C97 (= C98), C105 (= C106)
Sites not aligning to the query:
4ej6A Crystal structure of a putative zinc-binding dehydrogenase (target psi-012003) from sinorhizobium meliloti 1021
33% identity, 97% coverage: 2:330/340 of query aligns to 2:329/343 of 4ej6A
- active site: C40 (= C40), G41 (= G41), T42 (= T42), H45 (= H45), H61 (= H62), E62 (= E63), C91 (= C92), C94 (= C95), C97 (= C98), C105 (= C106), R109 (≠ S110), P147 (= P147), C151 (≠ S151)
- binding zinc ion: C91 (= C92), C94 (= C95), C97 (= C98), C105 (= C106)
Sites not aligning to the query:
Q5JI69 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) (see paper)
32% identity, 91% coverage: 14:324/340 of query aligns to 18:333/350 of Q5JI69
3gfbA L-threonine dehydrogenase (tktdh) from the hyperthermophilic archaeon thermococcus kodakaraensis (see paper)
32% identity, 91% coverage: 14:324/340 of query aligns to 16:331/347 of 3gfbA
- active site: C40 (= C40), G41 (= G41), T42 (= T42), H45 (= H45), H65 (= H62), E66 (= E63), C95 (= C92), C98 (= C95), C101 (= C98), C109 (= C106), K113 (≠ S110), P151 (= P147), A155 (≠ S151)
- binding nicotinamide-adenine-dinucleotide: G173 (= G170), G175 (= G172), P176 (= P173), L177 (≠ I174), S196 (≠ A192), E197 (≠ D193), P198 (≠ T194), R202 (= R198), F241 (≠ A237), S242 (≠ T238), A244 (≠ N240), L264 (≠ V260), G265 (= G261), L266 (= L262), I289 (≠ M282), T290 (≠ G283)
Sites not aligning to the query:
2ejvA Crystal structure of threonine 3-dehydrogenase complexed with NAD+
30% identity, 94% coverage: 4:323/340 of query aligns to 1:326/343 of 2ejvA
- active site: C38 (= C40), G39 (= G41), T40 (= T42), H43 (= H45), H63 (= H62), E64 (= E63), C93 (= C92), C96 (= C95), C99 (= C98), C107 (= C106), Q111 (≠ S110), P149 (= P147), A153 (≠ S151)
- binding nicotinamide-adenine-dinucleotide: G172 (= G170), G174 (= G172), P175 (= P173), I176 (= I174), S195 (≠ A192), D196 (= D193), P197 (≠ T194), R201 (= R198), F238 (≠ A237), S239 (≠ T238), N241 (= N240), A244 (= A243), L261 (≠ V260), G262 (= G261), I263 (≠ L262)
- binding zinc ion: C38 (= C40), H63 (= H62), E64 (= E63), C96 (= C95), C99 (= C98), C107 (= C106)
Sites not aligning to the query:
2dq4A Crystal structure of threonine 3-dehydrogenase
30% identity, 94% coverage: 4:323/340 of query aligns to 1:326/343 of 2dq4A
- active site: C38 (= C40), G39 (= G41), T40 (= T42), H43 (= H45), H63 (= H62), E64 (= E63), C93 (= C92), C96 (= C95), C99 (= C98), C107 (= C106), Q111 (≠ S110), P149 (= P147), A153 (≠ S151)
- binding zinc ion: C38 (= C40), H63 (= H62), E64 (= E63), C93 (= C92), C96 (= C95), C107 (= C106)
Sites not aligning to the query:
Q5SKS4 L-threonine 3-dehydrogenase; TDH; EC 1.1.1.103 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
30% identity, 94% coverage: 4:323/340 of query aligns to 1:326/343 of Q5SKS4
P07846 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Ovis aries (Sheep) (see paper)
34% identity, 94% coverage: 7:324/340 of query aligns to 10:333/354 of P07846
- C43 (= C40) binding
- Y49 (≠ A46) binding
- H67 (= H62) binding
- E68 (= E63) binding
- E153 (= E146) binding
- R296 (vs. gap) binding
- Y297 (vs. gap) binding
3qe3A Sheep liver sorbitol dehydrogenase (see paper)
34% identity, 94% coverage: 7:324/340 of query aligns to 6:330/351 of 3qe3A
- active site: C39 (= C40), G40 (= G41), S41 (≠ T42), H44 (= H45), H64 (= H62), E65 (= E63), R94 (≠ C92), D97 (≠ C95), C100 (= C98), S108 (≠ C106), F112 (≠ S110), P151 (= P147), G155 (≠ S151)
- binding glycerol: Y45 (≠ A46), F54 (vs. gap), T116 (≠ V114), R293 (vs. gap)
- binding zinc ion: C39 (= C40), H64 (= H62), E65 (= E63)
Sites not aligning to the query:
1pl6A Human sdh/nadh/inhibitor complex (see paper)
32% identity, 94% coverage: 7:324/340 of query aligns to 11:335/356 of 1pl6A
- active site: C44 (= C40), G45 (= G41), S46 (≠ T42), H49 (= H45), H69 (= H62), E70 (= E63), R99 (≠ C92), D102 (≠ C95), C105 (= C98), S113 (≠ C106), F117 (≠ S110), P156 (= P147), G160 (≠ S151)
- binding 4-[2-(hydroxymethyl)pyrimidin-4-yl]-n,n-dimethylpiperazine-1-sulfonamide: C44 (= C40), S46 (≠ T42), I56 (vs. gap), F59 (≠ Q51), H69 (= H62), E155 (= E146), L274 (= L262), F297 (≠ R285)
- binding nicotinamide-adenine-dinucleotide: G181 (= G172), P182 (= P173), I183 (= I174), D203 (= D193), L204 (≠ T194), R208 (= R198), C249 (≠ A237), T250 (= T238), V272 (= V260), G273 (= G261), L274 (= L262), F297 (≠ R285), R298 (vs. gap)
- binding zinc ion: C44 (= C40), H69 (= H62)
Sites not aligning to the query:
P07913 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Escherichia coli (strain K12) (see paper)
30% identity, 94% coverage: 4:324/340 of query aligns to 1:326/341 of P07913
- C38 (= C40) mutation to D: Shows only 1% of wild-type catalytic activity. This mutant can be stimulated to the wild-type activity level after incubation with Zn(+).; mutation to S: Loss of catalytic activity. This mutant cannot be stimulated to the wild-type activity level after incubation with Zn(+).
Q00796 Sorbitol dehydrogenase; SDH; (R,R)-butanediol dehydrogenase; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Ribitol dehydrogenase; RDH; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.4; EC 1.1.1.14; EC 1.1.1.56; EC 1.1.1.9 from Homo sapiens (Human) (see 10 papers)
32% identity, 94% coverage: 7:324/340 of query aligns to 12:336/357 of Q00796
- C45 (= C40) binding
- H70 (= H62) binding
- E71 (= E63) binding
- R110 (= R102) to P: in SORDD; results in protein aggregation
- H135 (≠ Y123) to R: in SORDD; results in protein aggregation
- A153 (= A143) to D: in SORDD; uncertain significance; results in protein aggregation; dbSNP:rs145813597
- I184 (= I174) binding
- D204 (= D193) binding
- R209 (= R198) binding
- Q239 (= Q225) to L: in dbSNP:rs1042079
- N269 (≠ T256) to T: in dbSNP:rs930337
- VGL 273:275 (= VGL 260:262) binding
- VFR 297:299 (≠ SR- 284:285) binding
- V322 (≠ L310) to I: in SORDD; uncertain significance; dbSNP:rs149975952
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
7y9pA Xylitol dehydrogenase s96c/s99c/y102c mutant(thermostabilized form) from pichia stipitis (see paper)
31% identity, 94% coverage: 7:324/340 of query aligns to 6:341/357 of 7y9pA
5vm2A Crystal structure of eck1772, an oxidoreductase/dehydrogenase of unknown specificity involved in membrane biogenesis from escherichia coli
29% identity, 97% coverage: 8:338/340 of query aligns to 7:343/347 of 5vm2A
- active site: C39 (= C40), G40 (= G41), S41 (≠ T42), H44 (= H45), H65 (= H62), E66 (= E63), C95 (= C92), C98 (= C95), C101 (= C98), C109 (= C106), D113 (≠ S110), P153 (= P147), G157 (≠ S151), K340 (= K335)
- binding magnesium ion: H65 (= H62), E66 (= E63), E152 (= E146)
- binding zinc ion: C95 (= C92), C98 (= C95), C101 (= C98), C109 (= C106)
P27867 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Rattus norvegicus (Rat) (see paper)
30% identity, 94% coverage: 7:324/340 of query aligns to 12:336/357 of P27867
4uejA Closed state of galactitol-1-phosphate 5-dehydrogenase from e. Coli in complex with glycerol. (see paper)
30% identity, 90% coverage: 24:330/340 of query aligns to 22:336/346 of 4uejA
- active site: C38 (= C40), G39 (= G41), S40 (≠ T42), P43 (≠ H45), H59 (= H62), E60 (= E63), C89 (= C92), C92 (= C95), C95 (= C98), C103 (= C106), D107 (≠ S110), P145 (= P147), G149 (≠ S151)
- binding glycerol: H59 (= H62), E144 (= E146)
- binding zinc ion: C89 (= C92), C92 (= C95), C95 (= C98), C103 (= C106)
Sites not aligning to the query:
Query Sequence
>18382 FitnessBrowser__Keio:18382
MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDIHAWGGNQPFFSYPRVL
GHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGRTNCCEKISVIGVHQDGGF
SEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAIAPGEQVLVVGAGPIGLGAAA
IAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDFDAQLRAQFGGSLAQKVIDATGN
QHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHKKETTMMGSRNATPEDFAKVGRLMA
EGKITADMMLTHRYPFATLAETYERDVINNRELIKGVITF
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory