SitesBLAST

C38 (= C39) mutation to S: No activity.
T40 (≠ S41) mutation to A: No activity.; mutation to S: Little decrease in activity.
H43 (≠ K44) mutation to A: No activity.; mutation to R: Higher level of activity at pH 9.

3meqA Crystal structure of alcohol dehydrogenase from brucella melitensis
24% identity, 82% coverage: 1:349/425 of query aligns to 3:323/341 of 3meqA

query
sites
3meqA

M

M

K

K

T

A

K

A

V

V

A

V

A

R

I

A

Y

F

G

G

K

K

R

-

D

P

V

L

R

T

L

I

R

D

V

E

F

V

E

P

L

I

P

P

E

Q

I

P

T

G

D

P

N

G

E

Q

L

I

L

Q

V

V

S

A

V

I

I

Q

S

A

D

S

S

G

V

V

C
|
C

L
x
H

S
x
T

T

D

W

L

K
x
H

A

A

L

E

L

G

G

D

S

W

E

P

H

V

K

K

R

-

V

-

P

-

D

-

L

-

E

P

N

N

H

P

P

P

V

F

I

I

T

P

G

G

H
|
H

E
|
E

C

G

A

V

G

G

V

F

I

V

V

S

E

A

V

V

G

G

K

S

N

G

L

V

T

K

G

-

K

H

Y

V

K

K

K

E

G

G

Q

D

R

R

F

-

V

-

L

-

Q

-

P

-

A

-

M

V

G

G

L

I

P

P

S

W

G

L

Y

Y

S

T

A

A

-
x
C

G

G

Y

H

S
x
C

Y

R

E

H

Y
x
C

F

L

G

G

N

-

A

-

T

-

Y

W

M

E

I

T

I

L

P
x
C

E

E

I

E

A

Q

I
x
L

N

N

L

T

G

G

C

Y

V

S

L

V

P

-

Y

-

H

-

G

N

S

G

Y

G

F

F

A

A

A

E

A

Y

S

V

L

V

A

A

E

D

P

P

M

N

C

-

I

F

I

V

G

G

A

H

Y

L

H

P

A

K

N

N

Y

I

H

D

-

F

-

N

-

E

-

I

-

A

-

P

-

V

-

L

-
x
C

-

A

-

G

T

V

T
|
T

Q

V

Y

Y

V

K

Y

G

E

L

H

K

R

V

M

T

G

D

V

T

K

K

P

P

G

G

G

-

N

D

I

W

A

V

L

V

L

I

A

S

C

G

A

I

G
|
G

P
x
G

M
x
L

G

G

I

H

G

M

A

A

I

V

D

Q

Y

Y

A

A

I

R

N

A

G

M

G

G

I

L

Q

N

P

-

S

-

R

-

V

V

V

A

V

V

D
|
D

I
|
I

D

D

K

R

R
x
K

L

L

A

D

Q

L

V

A

Q

R

K

R

L

L

L

G

P

A

V

T

E

-

L

-

A

-

S

-

K

-

G

-

I

-

E

-

L

-

V

V

Y

T

V

V

N

N

T

A

K

K

G

T

M

V

S

A

D

D

P

P

V

A

Q

A

M

Y

L

I

R

R

A

K

L

E

T

T

G

-

D

D

A

G

G

G

F

A

D

Q

D

G

I

V

F

L

V

V

Y
x
T

A
|
A

A
x
V

V
x
S

P

P

A

K

V
x
A

V

F

E

E

M

Q

A

A

D

L

E

G

L

M

L

V

A

A

E

R

D

G

G

G

C

T

L

V

N

S

F

L

F
x
N

A
x
G

G
x
L

P

P

T

P

D

G

K

-

N

-

F

-

K

D

V

F

P

P

F

L

N

S

F

I

Y

F

N

N

V

M

H

V

Y

L

N

N

S

G

T

V

H

T

V

V

V

R

G

G

T

S

S
x
I

G
x
V

G

G

S

T

T

R

D

L

D

D

M

L

K

Q

E

E

A

S

I

L

A

D

L

F

S

A

A

A

T

D

G

G

Q

K

L

V

Q

K

P

A

S

T

F

I

M

Q

V

T

T

G

H

K

I

L

G

E

G

D

L

I

D

N

A

A

V

I

active site: C40 (= C39), H41 (≠ L40), T42 (≠ S41), H45 (≠ K44), H63 (= H68), E64 (= E69), C94 (vs. gap), C97 (≠ S108), C100 (≠ Y111), C108 (≠ P122), L112 (≠ I126), C150 (vs. gap), T154 (= T162)
binding 1,4-dihydronicotinamide adenine dinucleotide: C40 (= C39), H41 (≠ L40), T42 (≠ S41), H45 (≠ K44), C150 (vs. gap), T154 (= T162), G176 (= G185), G177 (≠ P186), L178 (≠ M187), D197 (= D209), I198 (= I210), K202 (≠ R214), T241 (≠ Y264), A242 (= A265), V243 (≠ A266), S244 (≠ V267), A247 (≠ V270), N264 (≠ F287), G265 (≠ A288), L266 (≠ G289), I289 (≠ S315), V290 (≠ G316)
binding zinc ion: C40 (= C39), H63 (= H68), C94 (vs. gap), C97 (≠ S108), C100 (≠ Y111), C108 (≠ P122), C150 (vs. gap)

Sites not aligning to the query:

active site: 335

P27867 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Rattus norvegicus (Rat) (see paper)
26% identity, 50% coverage: 8:221/425 of query aligns to 14:216/357 of P27867

query
sites
P27867

I

V

Y

H

G

G

K

P

R

G

D

D

V

I

R

R

L

L

R

E

V

N

F

Y

E

P

L

I

P

P

E

E

I

L

T

G

D

P

N

N

E

D

L

V

L

L

V

L

S

K

V

M

I

H

S

S

D

V

S

G

V

I

C
|
C

L

G

S

S

T

D

-

V

-

H

-

Y

W

W

K

-

A

-

L

-

G

-

S

-

E

E

H

H

K

G

R

R

V

I

P

G

D

D

D

F

L

V

E

V

N

K

H

K

P

P

V

M

I

V

T

L

G

G

H
|
H

E
|
E

C

A

A

A

G

G

V

T

I

V

V

T

E

K

V

V

G

G

K

P

N

-

L

M

T

V

G

K

K

H

Y

L

K

K

K

P

G

G

Q

D

R

R

F

V

V

A

L

I

Q

E

P

P

A

-

M

-

G

G

L

V

P

P

S

R

G

-

Y

-

S

-

A

-

G

-

Y

-

S

E

Y

I

E

D

Y

E

F

F

G

C

G

K

N

I

A

G

T

R

Y

Y

M

N

I

L

I

T

P

P

E

S

I

I

A

F

I

F

-

C

-

A

-

T

-

P

-

D

-

G

N

N

L

L

G

-

C

C

V

R

L

F

P

Y

Y

K

H

H

G

S

S

A

-

D

-

F

-

C

-

Y

-

K

-

L

-

P

-

D

-

S

-

V

Y

T

F

F

A

E

A

G

S

A

L

L

A

I

E
|
E

P

P

M

L

C

-

I

-

G

-

A

-

Y

-

H

-

A

-

N

-

Y

-

H

-

T

-

T

S

Q

V

Y

G

V

I

Y

Y

E

A

H

C

R

R

M

R

G

G

V

S

K

V

P

S

G

L

G

G

N

N

I

K

A

V

L

L

L

V

A

C

C

G

A

A

G

G

P

P

M

I

G

G

I

I

G

-

A

-

I

V

D

T

Y

L

A

L

I

V

N

A

G

K

G

A

I

M

Q

G

P

A

S

S

R

Q

V

V

V

I

D
|
D

I

L

D

S

D

A

K

S

R
|
R

L

L

A

A

Q

K

V

A

Q

K

K

E

L

V

C45 (= C39) binding
H70 (= H68) binding
E71 (= E69) binding
E156 (= E146) binding
D204 (= D209) binding
R209 (= R214) binding

Sites not aligning to the query:

273:275 binding
297:299 binding

6iqdA Crystal structure of alcohol dehydrogenase from geobacillus stearothermophilus (see paper)
26% identity, 77% coverage: 11:336/425 of query aligns to 10:306/336 of 6iqdA

query
sites
6iqdA

K

K

R

K

D

A

V

L

R

E

L

I

R

K

V

E

F

V

E

E

L

R

P

P

E

K

I

L

T

E

D

E

N

G

E

E

L

V

L

L

V

V

S

K

V

I

I

E

S

A

D

C

S

G

V

V

C
|
C

L

H

S
x
T

T

D

W

L

K
x
H

A

A

L

-

G

-

S

-

E

-

H

H

K

G

R

D

V

W

P

P

D

I

D

K

L

-

E

P

N

K

H

L

P

P

V

L

I

I

T

P

G

G

H
|
H

E
|
E

C

G

A

V

G

G

V

I

I

V

V

V

E

E

V

V

G

A

K

K

N

G

L

V

T

K

G

-

K

S

Y

I

K

K

K

V

G

G

Q

D

R

R

F

-

V

-

L

-

Q

-

P

-

A

-

M

V

G

G

L

I

P

P

S

W

G

L

Y

Y

S

S

A

A

G
x
C

Y

G

S

E

Y
x
C

E

E

Y

Y

-
x
C

F

L

G

T

G

G

N

Q

A

E

T

T

Y

L

M

-

I

-

P

-

E

-

I

-

A

-

I

-

N

-

L

-

G

-

C
|
C

V

P

L

H

P

Q

Y

L

H

N

G

G

S

G

Y

Y

F

S

A

V

A

D

A

G

S

G

L

Y

A

A

E

E

P

-

M

Y

C

C

C

K

I

A

I

P

G

A

A

D

Y

Y

H

V

A

A

N

K

Y

I

-

P

-

D

-

N

-

L

-

D

-

P

-

V

-

E

-

V

-

A

-

P

-

I

-

L

-
x
C

-

A

H

G

T

V

T

T

Q

T

Y

Y

V

K

Y

A

E

L

H

K

R

V

M

S

G

G

V

A

K

R

P

P

G

G

G

E

N

W

I

V

A

A

L

I

L

Y

A

G

C

I

A

G

G

G

P

-

M

L

G

G

I

H

G

I

A

A

I

L

D

Q

Y

Y

A

A

I

K

N

A

G

M

G

G

I

L

Q

N

P

-

S

-

R

-

V

V

V

A

V

V

D

D

I

I

D

S

D

D

K

E

R

K

L

-

A

-

Q

-

V

-

Q

-

K

-

L

-

P

-

V

S

E

K

L

L

A

A

A

K

S

D

K

L

G

G

I

A

E

D

L

I

V

A

Y

I

V

N

N

G

T

L

K

K

G

-

M

-

S

E

D

D

P

P

V

V

Q

K

M

A

L

I

R

H

A

D

L

Q

T

V

G

G

D

G

A

-

G

-

F

-

D

-

I

-

F

-

V

V

Y

H

A

A

V

I

P

S

A

V

V

A

V

V

-

N

-

K

-

A

-

F

E

E

M

Q

A

A

D

Y

E

Q

L

S

L

V

A

K

E

R

D

G

G

G

C

T

L

L

N

V

F

V

A

G

G

L

P

P

T

-

D

-

K

-

N

N

F

A

K

D

V

L

P

P

F

I

N

P

F

I

Y

F

N

D

V

T

H

V

Y

L

N

N

S

G

T

V

H

S

V

V

V

K

G

G

T

S

S

I

G

V

G

G

S

T

T

R

D

K

D

D

M

M

K

Q

E

E

A

A

I

L

A

D

L

F

S

A

A

A

T

R

G

G

Q

K

L

V

Q

R

P

P

active site: C38 (= C39), T40 (≠ S41), H43 (≠ K44), H61 (= H68), C148 (vs. gap)
binding zinc ion: C38 (= C39), H61 (= H68), E62 (= E69), C92 (≠ G106), C95 (≠ Y109), C98 (vs. gap), C106 (= C130), C148 (vs. gap)

2eerB Structural study of project id st2577 from sulfolobus tokodaii strain7
22% identity, 82% coverage: 12:358/425 of query aligns to 11:336/347 of 2eerB

query
sites
2eerB

R

K

D

P

V

L

R

K

L

L

R

E

V

D

F

I

E

P

L

I

P

P

E

K

I

P

T

K

D

G

N

S

E

Q

L

V

L

L

V

I

S

K

V

I

I

E

S

A

D

A

S

G

V

V

C
|
C

L
x
H

S
|
S

T

D

W

V

K
x
H

A

M

A

R

L

Q

L

G

G

R

S

F

E

G

H

N

K

L

R

R

V

I

P

V

D

E

D

D

L

L

E

G

-

V

N

K

H

L

P

P

V

V

I

T

T

L

G

G

H
|
H

E
|
E

C

I

A

A

G

G

V

R

I

I

V

E

E

E

V

V

G

G

K

D

N

E

L

V

T

V

G

G

K

-

Y

Y

K

S

K

K

G

G

Q

D

R

L

F

V

V

A

L

V

Q

N

P

P

A

W

M

E

G

G

L
x
E

P

G

S

N

G
x
C

Y

Y

S

Y

A
x
C

G

R

Y

I

S

G

Y

E

E

E

Y

H

F

L

G
x
C

G

D

N

S

A

P

T
x
R

Y

W

M

L

I

G

I

I

P

N

E

Y

I

D

A

G

I

A

N

Y

L

A

G

E

C

Y

V

V

L

L

P

V

Y

P

H

H

G

Y

S

K

Y

Y

F

L

-

Y

-

K

-

L

-

R

A

L

A

S

A

A

S

V

L

E

A

A

E

A

P

P

M

L

C

T

C
|
C

I

-

G

-

A

-

Y

-

H

-

A

-

N

-

Y

S

H

G

T

V

T
|
T

Q

T

Y

Y

V

R

Y

A

E

V

H

R

R

K

M

A

G

S

V

L

K

D

P

P

G

S

G

K

N

T

I

L

A

V

L

V

L

I

A
x
G

C

A

A

G

G
|
G

P

-

M

-

G
|
G

I
x
L

G

G

A

T

I

M

D

A

Y

I

A

Q

I

I

N

A

G

K

G

A

I

V

Q

S

P

G

S

A

R

T

V

I

V

G

V

V

D
|
D

I
x
V

D
x
R

D

E

K

E

R

A

L

L

A

-

Q

-

V

-

Q

-

K

-

L

-

P

-

V

-

E

E

L

A

A

A

A

K

S

R

K

A

G

G

I

A

E

D

L

-

V

-

Y

Y

V

V

N

I

T

N

K

A

G

S

M

S

S

Q

D

D

P

P

V

V

Q

S

M

E

L

I

R

R

A

R

L

I

T

T

G

Q

D

G

A

K

G

G

F

A

D

D

D

A

I

V

F

I

V

D

Y
x
L

A
x
N

A

N

V

S

P

E

A

K

V

T

V

L

E

S

M

I

A

Y

D

P

E

Y

L

V

L

L

A

A

E

K

D

Q

G

G

-

K

-

Y

-

V

C

M

L
x
V

N
x
G

F
x
L

F
|
F

A

G

G

-

P

-

T

A

D

D

K

L

N

K

F

Y

K

H

V

A

P

P

F

L

N

-

F

-

Y

-

N

-

V

I

H

T

Y

L

N

N

S

E

T

V

H

Q

V

F

V

I

G

G

T

S

S
x
L

G
x
V

G

G

S

N

T

Q

D

S

D

D

M

F

K

L

E

G

A

I

I

M

A

S

L

L

S

A

A

E

T

A

G

G

Q

K

L

V

Q

K

P

P

S

-

F

-

M

M

V

V

T

T

H

K

I

T

G

M

G

K

L

L

D

E

A

E

V

A

P

N

E

E

T

A

V

I

L

D

N

N

L

L

P

E

D

N

active site: C38 (= C39), H39 (≠ L40), S40 (= S41), H43 (≠ K44), H68 (= H68), E69 (= E69), E98 (≠ L99), C101 (≠ G102), C104 (≠ A105), C112 (≠ G113), R116 (≠ T117), C154 (= C150), T158 (= T162)
binding nicotinamide-adenine-dinucleotide: C38 (= C39), H39 (≠ L40), S40 (= S41), H43 (≠ K44), C154 (= C150), T158 (= T162), G178 (≠ A182), G181 (= G185), G182 (= G188), L183 (≠ I189), D203 (= D209), V204 (≠ I210), R205 (≠ D211), L247 (≠ Y264), N248 (≠ A265), V270 (≠ L284), G271 (≠ N285), L272 (≠ F286), F273 (= F287), L295 (≠ S315), V296 (≠ G316)
binding zinc ion: E98 (≠ L99), C101 (≠ G102), C104 (≠ A105), C112 (≠ G113)

Sites not aligning to the query:

active site: 342
binding nicotinamide-adenine-dinucleotide: 342

1rjwA Crystal structure of NAD(+)-dependent alcohol dehydrogenase from bacillus stearothermophilus strain lld-r (see paper)
25% identity, 84% coverage: 1:355/425 of query aligns to 1:325/339 of 1rjwA

query
sites
1rjwA

M

M

K

K

T

A

K

A

V

V

A

V

A

E

I

Q

Y

F

G

-

K

K

R

E

D

P

V

L

R

K

L

I

R

K

V

E

F

V

E

E

L

K

P

P

E

T

I

I

T

S

D

Y

N

G

E

E

L

V

L

L

V

V

S

R

V

I

I

K

S

A

D

C

S

G

V

V

C
|
C

L
x
H

S
x
T

T

D

W

L

K
x
H

A

A

L

-

G

-

S

-

E

-

H

H

K

G

R

D

V

W

P

P

D

V

D

K

L

-

E

P

N

K

H

L

P

P

V

L

I

I

T

P

G

G

H
|
H

E
|
E

C

G

A

V

G

G

V

I

I

V

V

E

E

E

V

V

G

G

K

P

N

G

L

V

T

T

G

-

K

H

Y

L

K

K

K

V

G

G

Q

D

R

R

F

-

V

-

L

-

Q

-

P

-

A

-

M

V

G

G

L

I

P

P

S

W

G

L

Y

Y

S

S

A

A

-
x
C

-

G

-

H

-
x
C

-

D

-

Y

-
x
C

-

L

-

S

-

G

-

Q

-

E

-

T

-

L

-
x
C

-

E

-

H

-

Q

-
x
K

-

N

-

A

G

G

Y

Y

S

S

Y

V

E

D

Y

-

F

-

G

G

N

Y

A

A

T

E

Y

Y

M

C

I

-

P

-

E

R

I

A

A

A

I

A

N

D

L

Y

G

V

C

V

V

K

L

I

P

P

Y

D

H

N

G

L

S

S

Y

F

F

E

A

E

A

A

S

P

L

I

A

-

E

-

P

-

M

F

C
|
C

C

A

I

G

I

V

G
x
T

A

T

Y

Y

H

K

A

A

N

L

Y

K

H

V

T

T

T

-

Q

-

Y

-

V

-

Y

-

E

-

H

-

R

-

M

-

G

G

V

A

K

K

P

P

G

G

G

E

N

W

I

V

A

A

L

I

L

Y

A

G

C

I

A

G

G

G

P

-

M

L

G

G

I

H

G

V

A

A

I

V

D

Q

Y

Y

A

A

I

K

N

A

G

M

G

G

I

L

Q

N

P

-

S

-

R

-

V

V

V

A

V

V

D

D

I

I

D

G

D

D

K

E

R

K

L

L

A

-

Q

-

V

-

Q

-

K

-

L

-

P

-

V

-

E

E

L

L

A

A

A

K

S

E

K

L

G

G

I

A

E

D

L

L

V

V

Y

V

V

N

N

P

T

L

K

K

G

-

M

-

S

E

D

D

P

A

V

A

Q

K

M

F

L

M

R

K

A

E

L

K

T

V

G

G

D

-

A

-

G

G

F

V

D

H

D

A

I

A

F

V

V

V

Y

T

A

A

A

V

V

S

P

K

A

P

V

A

V

F

E

Q

M

S

A

A

D

Y

E

N

L

S

L

I

A

R

E

R

D

G

G

G

C

A

L

C

N

V

F

L

F

V

A

G

G

L

P

P

T

P

D

E

K

E

N

-

F

-

K

-

V

M

P

P

F

I

N

P

F

I

Y

F

N

D

V

T

H

V

Y

L

N

N

S

G

T

I

H

K

V

I

G

G

T

S

S
x
I

G

V

G

G

S

T

T

R

D

K

D

D

M

L

K

Q

E

E

A

A

I

L

A

Q

L

F

S

A

A

A

T

E

G

G

Q

K

L

V

Q

K

P

T

S

I

F

I

M

E

V

V

T

Q

H

P

I

L

G

E

G

K

L

I

D

N

A

E

V

V

P

F

E

D

T

R

V

M

L

L

N

K

active site: C38 (= C39), H39 (≠ L40), T40 (≠ S41), H43 (≠ K44), H61 (= H68), E62 (= E69), C92 (vs. gap), C95 (vs. gap), C98 (vs. gap), C106 (vs. gap), K110 (vs. gap), C148 (= C149), T152 (≠ G153)
binding trifluoroethanol: T40 (≠ S41), C148 (= C149), I285 (≠ S315)
binding zinc ion: C38 (= C39), H61 (= H68), C92 (vs. gap), C95 (vs. gap), C98 (vs. gap), C106 (vs. gap)

Sites not aligning to the query:

active site: 331

3piiA Crystal structure of mutant of ht- alcohol dehydrogenase with substrate analogue butyramide
25% identity, 84% coverage: 1:355/425 of query aligns to 1:325/337 of 3piiA

query
sites
3piiA

M

M

K

K

T

A

K

A

V

V

A

V

A

E

I

Q

Y

F

G

-

K

K

R

E

D

P

V

L

R

K

L

I

R

K

V

E

F

V

E

E

L

K

P

P

E

T

I

I

T

S

D

Y

N

G

E

E

L

V

L

L

V

V

S

R

V

I

I

K

S

A

D

C

S

G

V

V

C
|
C

L
x
H

S
x
T

T

D

W

L

K
x
H

A

A

L

-

G

-

S

-

E

-

H

H

K

G

R

D

V

W

P

P

D

V

D

K

L

-

E

P

N

K

H

L

P

P

V

L

I

I

T

P

G

G

H
|
H

E
|
E

C

G

A

V

G

G

V

I

I

V

V

E

E

E

V

V

G

G

K

P

N

G

L

V

T

T

G

-

K

H

Y

L

K

K

K

V

G

G

Q

D

R

R

F

-

V

-

L

-

Q

-

P

-

A

-

M

V

G

G

L

I

P

P

S
x
W

G

L

Y

Y

S

S

A

A

-
x
C

-

G

-

H

-
x
C

-

D

-

Y

-
x
C

-

L

-

S

-

G

-

Q

-

E

-

T

-

L

-
x
C

-

E

-

H

-

Q

-
x
K

-

N

-

A

G

G

Y

Y

S

S

Y

V

E

D

Y

-

F

-

G

G

N

Y

A

A

T

E

Y

Y

M

C

I

-

P

-

E

R

I

A

A

A

I

A

N

D

L

Y

G

V

C

V

V

K

L

I

P

P

Y

D

H

N

G

L

S

S

Y

F

F

E

A

E

A

A

S

P

L

I

A

-

E

-

P

-

M

F

C
|
C

C

A

I

G

I

V

G
x
T

A

T

Y

Y

H

K

A

A

N

L

Y

K

H

V

T

T

T

-

Q

-

Y

-

V

-

Y

-

E

-

H

-

R

-

M

-

G

G

V

A

K

K

P

P

G

G

G

E

N

W

I

V

A

A

L

I

L

Y

A

G

C

I

A

G

G

G

P

-

M

F

G

G

I

H

G

V

A

A

I

V

D

Q

Y

Y

A

A

I

K

N

A

G

M

G

G

I

L

Q

N

P

-

S

-

R

-

V

V

V

A

V

V

D

D

I

I

D

G

D

D

K

E

R

K

L

L

A

-

Q

-

V

-

Q

-

K

-

L

-

P

-

V

-

E

E

L

L

A

A

A

K

S

E

K

L

G

G

I

A

E

D

L

L

V

V

Y

V

V

N

N

P

T

L

K

K

G

-

M

-

S

E

D

D

P

A

V

A

Q

K

M

F

L

M

R

K

A

E

L

K

T

V

G

G

D

-

A

-

G

G

F

V

D

H

D

A

I

A

F

V

V

V

Y

T

A

A

A

V

V

S

P

K

A

P

V

A

V

F

E

Q

M

S

A

A

D

Y

E

N

L

S

L

I

A

R

E

R

D

G

G

G

C

A

L

C

N

V

F

L

F

V

A

G

G

L

P

P

T

P

D

E

K

E

N

-

F

-

K

-

V

M

P

P

F

I

N

P

F

I

Y

F

N

D

V

T

H

V

Y

L

N

N

S

G

T

I

H

K

V

I

G

G

T

S

S

I

G

V

G

G

S

T

T

R

D

K

D

D

M

L

K

Q

E

E

A

A

I

L

A

Q

L

F

S

A

A

A

T

E

G

G

Q

K

L

V

Q

K

P

T

S

I

F

I

M

E

V

V

T

Q

H

P

I

L

G

E

G

K

L

I

D

N

A

E

V

V

P

F

E

D

T

R

V

M

L

L

N

K

active site: C38 (= C39), H39 (≠ L40), T40 (≠ S41), H43 (≠ K44), H61 (= H68), E62 (= E69), C92 (vs. gap), C95 (vs. gap), C98 (vs. gap), C106 (vs. gap), K110 (vs. gap), C148 (= C149), T152 (≠ G153)
binding butyramide: T40 (≠ S41), H61 (= H68), W87 (≠ S101), C148 (= C149)
binding zinc ion: C38 (= C39), H61 (= H68), E62 (= E69), C92 (vs. gap), C95 (vs. gap), C98 (vs. gap), C106 (vs. gap), C148 (= C149)

Sites not aligning to the query:

active site: 331

3qe3A Sheep liver sorbitol dehydrogenase (see paper)
25% identity, 50% coverage: 8:221/425 of query aligns to 8:210/351 of 3qe3A

query
sites
3qe3A

I

V

Y

H

G

G

K

P

R

G

D

D

V

L

R

R

L

L

R

E

V

N

F

Y

E

P

L

I

P

P

E

E

I

P

T

G

D

P

N

N

E

E

L

V

L

L

V

L

S

K

V

M

I

H

S

S

D

V

S

G

V

I

C
|
C

L
x
G

S
|
S

T

D

-

V

-
x
H

-
x
Y

W

W

K

-

A

-

L

-

G

-

S

-

E

Q

H

H

K

G

R

R

V

I

P

G

D

D

D
x
F

L

V

E

V

N

K

H

K

P

P

V

M

I

V

T

L

G

G

H
|
H

E
|
E

C

A

A

S

G

G

V

T

I

V

V

V

E

K

V

V

G

G

K

-

N

S

L

L

T

V

G

R

K

H

Y

L

K

Q

K

P

G

G

Q

D

R

R

F

V

V

A

L

I

Q

Q

P

P

A

G

M

-

G

-

L

-

P

-

S

-

G

-

Y

-

S

-

A

A

G

P

Y
x
R

S

Q

Y

T

E
x
D

Y

E

F

F

G
x
C

G

K

N

I

A

G

T

R

Y

Y

M

N

I

L

I
x
S

P

P

E

T

I

I

A
x
F

I

F

-

C

-

A

-
x
T

-

P

-

D

-

G

N

N

L

L

G

C

C

R

V

F

L

Y

P

K

Y

H

H

N

G

A

S

N

Y

F

-

C

-

Y

-

K

-

L

-

P

-

D

-

N

-

V

-

T

F

F

A

E

A

G

S

A

L

L

A

I

E

E

P
|
P

M

L

C

S

C

V

I
x
G

I

I

G

H

A

A

Y

C

H

-

A

-

N

-

Y

-

H

-

T

-

Q

-

Y

-

V

-

Y

-

E

-

H

R

R

R

M

A

G

G

V

V

K

T

P

L

G

-

G

G

N

N

I

K

A

V

L

L

L

V

A

C

C

G

A

A

G

G

P

P

M

I

G

G

I

L

G

-

A

-

I

V

D

N

Y

L

A

L

I

A

N

A

G

K

G

A

I

M

Q

G

P

A

S

A

R

Q

V

V

V

T

D

D

I

L

D

S

D

A

K

S

R

R

L

L

A

S

Q

K

V

A

Q

K

K

E

L

V

active site: C39 (= C39), G40 (≠ L40), S41 (= S41), H44 (vs. gap), H64 (= H68), E65 (= E69), R94 (≠ Y107), D97 (≠ E110), C100 (≠ G113), S108 (≠ I121), F112 (≠ A125), P151 (= P147), G155 (≠ I151)
binding glycerol: Y45 (vs. gap), F54 (≠ D58), T116 (vs. gap)
binding zinc ion: C39 (= C39), H64 (= H68), E65 (= E69)

Sites not aligning to the query:

active site: 339
binding glycerol: 293

1e3jA Ketose reductase (sorbitol dehydrogenase) from silverleaf whitefly (see paper)
29% identity, 28% coverage: 6:124/425 of query aligns to 5:110/348 of 1e3jA

query
sites
1e3jA

A

A

A

V

I

L

Y

Y

G

K

K

Q

R

N

D

D

V

L

R

R

L

L

R

E

V

Q

F

R

E

P

L

I

P

P

E

E

I

P

T

K

D

E

N

D

E

E

L

V

L

L

V

L

S

Q

V

M

I

A

S

Y

D

V

S

G

V

I

C
|
C

L
x
G

S
|
S

T

D

W

V

K
x
H

A

Y

A

-

L

-

G

-

S

Y

E

E

H

H

K

G

R

R

V

I

P

A

D

D

D

F

L

I

E

V

N

K

H

D

P

P

V

M

I

V

T

I

G

G

H
|
H

E
|
E

C

A

A

S

G

G

V

T

I

V

V

V

E

K

V

V

G

G

K

K

N

N

L

V

T

K

G

-

K

H

Y

L

K

K

G

G

Q

D

R

R

F

V

V

A

L

V

Q

E

P

P

A

-

M

-

G

G

L

V

P

P

S
x
C

G

R

Y

R

S
x
C

A

-

G

-

Y

-

S

-

Y

-

E

-

Y

Q

F

F

G
x
C

G

K

N

E

A

G

T

K

Y

Y

M

N

I

L

I
x
C

P

P

E

D

I

L

active site: C38 (= C39), G39 (≠ L40), S40 (= S41), H43 (≠ K44), H63 (= H68), E64 (= E69), C93 (≠ S101), C96 (≠ S104), C99 (≠ G113), C107 (≠ I121)
binding zinc ion: C38 (= C39), H63 (= H68), E64 (= E69), C93 (≠ S101), C96 (≠ S104), C99 (≠ G113), C107 (≠ I121)

Sites not aligning to the query:

active site: 111, 150, 154, 341
binding phosphate ion: 174, 196, 197, 198, 201

4z6kA Alcohol dehydrogenase from the antarctic psychrophile moraxella sp. Tae 123
25% identity, 79% coverage: 1:337/425 of query aligns to 1:307/345 of 4z6kA

query
sites
4z6kA

M

M

K

K

T

A

K

A

V

V

A

L

A

H

I

E

Y

F

G

G

K

-

R

Q

D

S

V

L

R

Q

L

I

R

E

V

E

F

V

E

D

L

I

P

P

E

T

I

P

T

G

D

A

N

G

E

E

L

I

L

V

V

V

S

K

V

M

I

Q

S

A

D

S

S

G

V

V

C
|
C

L
x
H

S
x
T

T

D

W

L

K
x
H

A

A

A

V

L

E

L

G

G

D

S

W

E

P

H

V

K

K

R

P

V

S

P

P

D

-

L

-

E

-

N

-

H

-

P

P

V

F

I

I

T

P

G

G

H
|
H

E
|
E

C

G

A

V

G

G

V

L

I

I

V

T

E

A

V

V

G

G

K

E

N

G

L

V

T

T

G

-

K

H

Y

V

K

K

K

E

G

G

Q

D

R

R

F

V

-

G

-

V

-

A

V

W

L

L

Q

Y

P

S

A

A

M
x
C

G

G

-

H

-
x
C

-

T

-

H

-
x
C

-

L

-

G

-

W

-

E

-

T

-

L

-
x
C

-

E

-

S

-

Q

L
x
Q

P

N

S

S

G

G

Y

Y

S

S

A

V

G

N

Y

G

S

S

Y

F

-

A

E

E

Y

Y

F

V

G

L

G

A

N

N

A

A

T

N

Y

Y

M

V

-

G

I

I

P

P

E

E

I

S

A

V

I

D

N

S

L

I

G

-

C

-

V

-

L

-

P

-

Y

-

H

-

G

-

S

-

Y

-

F

-

A

-

S

E

L

I

A

A

E

P

P

V

M

L

C
|
C

C

A

I

G

I

V

G
x
T

A

V

Y

Y

H

K

A

G

N

L

Y

K

H

M

T

T

T

D

Q

-

Y

-

V

-

Y

-

E

-

H

-

R

-

M

-

G

-

V

T

K

K

P

P

G

-

G

G

N

D

I

W

A

V

L

V

L

I

A

S

C

G

A

I

G

G

P

G

M

L

G

G

I

H

G

M

A

A

I

V

D

Q

Y

Y

A

A

I

I

N

A

G

M

G

G

I

L

Q

N

P

-

S

-

R

-

V

V

V

A

V

V

D

D

I

I

D

D

K

D

R

K

L

L

A

A

Q

F

V

A

Q

K

K

K

L

L

L

-

P

-

V

-

E

-

L

-

A

-

A

G

S

A

K

K

G

-

I

-

E

-

L

-

V

V

Y

T

V

V

N

N

T

A

K

K

G

N

M

-

S

T

D

D

P

P

V

A

Q

E

M

Y

L

L

R

Q

A

K

L

E

T

I

G

G

D

-

A

-

G

G

F

A

D

H

D

G

I

A

F

L

V

V

Y

T

A

A

A

V

V

S

P

A

A

K

V

A

V

F

E

D

M

Q

A

A

D

L

E

S

L

M

L

L

A

R

E

R

D

G

G

G

C

T

L

L

N

V

F

C

F

N

A

G

G

L

P

P

T

P

D

G

K

-

N

-

F

-

K

D

V

F

P

P

F

V

N

S

F

I

Y

F

N

D

V

T

H

V

Y

L

N

N

S

G

T

I

H

T

V

I

V

R

G

G

T

S

S

I

G

V

G

G

S

T

T

R

D

L

D

D

M

L

K

Q

E

E

A

S

I

L

A

D

L

M

S

A

A

A

T

A

G

G

Q

K

L

V

Q

K

P

A

S

T

active site: C38 (= C39), H39 (≠ L40), T40 (≠ S41), H43 (≠ K44), H61 (= H68), E62 (= E69), C92 (≠ M97), C95 (vs. gap), C98 (vs. gap), C106 (vs. gap), Q110 (≠ L99), C148 (= C149), T152 (≠ G153)
binding zinc ion: C38 (= C39), H61 (= H68), C92 (≠ M97), C95 (vs. gap), C98 (vs. gap), C106 (vs. gap), C148 (= C149)

Sites not aligning to the query:

active site: 331

Q8GIX7 Alcohol dehydrogenase; ADH; EC 1.1.1.1 from Moraxella sp. (strain TAE123) (see paper)
25% identity, 79% coverage: 1:337/425 of query aligns to 1:307/338 of Q8GIX7

query
sites
Q8GIX7

M

M

K

K

T

A

K

A

V

V

A

L

A

H

I

E

Y

F

G

G

K

-

R

Q

D

S

V

L

R

Q

L

I

R

E

V

E

F

V

E

D

L

I

P

P

E

T

I

P

T

G

D

A

N

G

E

E

L

I

L

V

V

V

S

K

V

M

I

Q

S

A

D

S

S

G

V

V

C
|
C

L

H

S

T

T

D

W

L

K

H

A

A

A

V

L

E

L

G

G

D

S

W

E

P

H

V

K

K

R

P

V

S

P

P

D

-

L

-

E

-

N

-

H

-

P

P

V

F

I

I

T

P

G

G

H
|
H

E
|
E

C

G

A

V

G

G

V

L

I

I

V

T

E

A

V

V

G

G

K

E

N

G

L

V

T

T

G

-

K

H

Y

V

K

K

K

E

G

G

Q

D

R

R

F

V

-

G

-

V

-

A

V

W

L

L

Q

Y

P

S

A

A

M
x
C

G

G

-

H

-
x
C

-

T

-

H

-
x
C

-

L

-

G

-

W

-

E

-

T

-

L

-
x
C

-

E

-

S

-

Q

L

Q

P

N

S

S

G

G

Y

Y

S

S

A

V

G

N

Y

G

S

S

Y

F

-

A

E

E

Y

Y

F

V

G

L

G

A

N

N

A

A

T

N

Y

Y

M

V

-

G

I

I

P

P

E

E

I

S

A

V

I

D

N

S

L

I

G

-

C

-

V

-

L

-

P

-

Y

-

H

-

G

-

S

-

Y

-

F

-

A

-

S

E

L

I

A

A

E

P

P

V

M

L

C
|
C

C

A

I

G

I

V

G

T

A

V

Y

Y

H

K

A

G

N

L

Y

K

H

M

T

T

T

D

Q

-

Y

-

V

-

Y

-

E

-

H

-

R

-

M

-

G

-

V

T

K

K

P

P

G

-

G

G

N

D

I

W

A

V

L

V

L

I

A

S

C

G

A

I

G

G

P

G

M

L

G

G

I

H

G

M

A

A

I

V

D

Q

Y

Y

A

A

I

I

N

A

G

M

G

G

I

L

Q

N

P

-

S

-

R

-

V

V

V

A

V

V

D

D

I

I

D

D

K

D

R

K

L

L

A

A

Q

F

V

A

Q

K

K

K

L

L

L

-

P

-

V

-

E

-

L

-

A

-

A

G

S

A

K

K

G

-

I

-

E

-

L

-

V

V

Y

T

V

V

N

N

T

A

K

K

G

N

M

-

S

T

D

D

P

P

V

A

Q

E

M

Y

L

L

R

Q

A

K

L

E

T

I

G

G

D

-

A

-

G

G

F

A

D

H

D

G

I

A

F

L

V

V

Y

T

A

A

A

V

V

S

P

A

A

K

V

A

V

F

E

D

M

Q

A

A

D

L

E

S

L

M

L

L

A

R

E

R

D

G

G

G

C

T

L

L

N

V

F

C

F

N

A

G

G

L

P

P

T

P

D

G

K

-

N

-

F

-

K

D

V

F

P

P

F

V

N

S

F

I

Y

F

N

D

V

T

H

V

Y

L

N

N

S

G

T

I

H

T

V

I

V

R

G

G

T

S

S

I

G

V

G

G

S

T

T

R

D

L

D

D

M

L

K

Q

E

E

A

S

I

L

A

D

L

M

S

A

A

A

T

A

G

G

Q

K

L

V

Q

K

P

A

S

T

C38 (= C39) binding
H61 (= H68) binding
E62 (= E69) binding
C92 (≠ M97) binding
C95 (vs. gap) binding
C98 (vs. gap) binding
C106 (vs. gap) binding
C148 (= C149) binding

P07846 Sorbitol dehydrogenase; SDH; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.14; EC 1.1.1.9 from Ovis aries (Sheep) (see paper)
24% identity, 50% coverage: 8:221/425 of query aligns to 12:213/354 of P07846

query
sites
P07846

I

V

Y

H

G

G

K

P

R

G

D

D

V

L

R

R

L

L

R

E

V

N

F

Y

E

P

L

I

P

P

E

E

I

P

T

G

D

P

N

N

E

E

L

V

L

L

V

L

S

K

V

M

I

H

S

S

D

V

S

G

V

I

C
|
C

L

G

S

S

T

D

-

V

-

H

-
x
Y

W

W

K

Q

A

G

A

-

L

-

G

-

S

-

E

-

H

-

K

-

R

R

V

I

P

G

D

D

D

F

L

V

E

V

N

K

H

K

P

P

V

M

I

V

T

L

G

G

H
|
H

E
|
E

C

A

A

S

G

G

V

T

I

V

V

V

E

K

V

V

G

G

K

-

N

S

L

L

T

V

G

R

K

H

Y

L

K

Q

K

P

G

G

Q

D

R

R

F

V

V

A

L

I

Q

Q

P

P

A

G

M

-

G

-

L

-

P

-

S

-

G

-

Y

-

S

-

A

A

G

P

Y

R

S

Q

Y

T

E

D

Y

E

F

F

G

C

G

K

N

I

A

G

T

R

Y

Y

M

N

I

L

I

S

P

P

E

T

I

I

A

F

I

F

-

C

-

A

-

T

-

P

-

D

-

G

N

N

L

L

G

C

C

R

V

F

L

Y

P

K

Y

H

H

N

G

A

S

N

Y

F

-

C

-

Y

-

K

-

L

-

P

-

D

-

N

-

V

-

T

F

F

A

E

A

G

S

A

L

L

A

I

E
|
E

P

P

M

L

C

S

C

V

I

G

I

I

G

H

A

A

Y

C

H

-

A

-

N

-

Y

-

H

-

T

-

Q

-

Y

-

V

-

Y

-

E

-

H

R

R

R

M

A

G

G

V

V

K

T

P

L

G

-

G

G

N

N

I

K

A

V

L

L

L

V

A

C

C

G

A

A

G

G

P

P

M

I

G

G

I

L

G

-

A

-

I

V

D

N

Y

L

A

L

I

A

N

A

G

K

G

A

I

M

Q

G

P

A

S

A

R

Q

V

V

V

T

D

D

I

L

D

S

D

A

K

S

R

R

L

L

A

S

Q

K

V

A

Q

K

K

E

L

V

C43 (= C39) binding
Y49 (vs. gap) binding
H67 (= H68) binding
E68 (= E69) binding
E153 (= E146) binding

Sites not aligning to the query:

296 binding
297 binding

5o9dA Crystal structure of r. Ruber adh-a, mutant y294f, w295a, y54f, f43h, h39y (see paper)
24% identity, 64% coverage: 63:334/425 of query aligns to 57:313/346 of 5o9dA

query
sites
5o9dA

P

P

V

L

I

T

T

L

G

G

H
|
H

E
|
E

C

G

A

V

G

G

V

T

I

V

V

A

E

E

V

L

G

G

K

E

N

G

L

V

T

T

G

G

K

-

Y

-

K

-

G

-

Q

-

R

-

F

F

V

G

L

V

Q

G

P

D

A

A

M

V

G

A

L

V

P

Y

S

G

G

P

Y

W

S

G

A
x
C

G

G

Y

A

S
x
C

Y

H

E

A

Y
x
C

F

A

G

R

G

G

N

R

A

E

T

N

Y

Y

M
x
C

I

-

P

-

E

T

I

R

A

A

I
x
A

N

D

L

L

G

G

C

I

V

T

L

P

P

P

Y

G

H

L

G

G

S

S

Y

-

F

-

A

-

A

P

A

G

S

S

L

M

A

A

E

E

P

Y

M

M

C

I

C

V

-

D

-

S

-

A

-

R

-

H

-

L

-

V

-

P

-

I

-

G

-

D

-

L

-

D

-

P

-

V

-

A

-

P

I

L

I

T

G
x
D

A

A

Y

G

H

L

A
x
T

N

P

Y

Y

H

H

T

A

T

I

Q

S

Y

R

V

V

Y

L

E

P

H

L

R

-

M

-

G

-

V

L

K

G

P

P

G

G

G

S

N

T

I

A

A

V

L

V

L

I

A

-

C

-

A

-

G

G

P
x
V

M
x
G

G
|
G

I
x
L

G

G

A

H

I

V

D

G

Y

I

A

Q

I

I

N

L

G

R

G

A

I

V

Q

S

P

A

S

A

R

R

V

V

V

I

V

A

V

V

D
|
D

I
x
L

D

D

K

D

R
|
R

L

L

A

A

Q

-

V

-

Q

-

K

-

L

-

P

-

V

-

E

-

L

L

A

A

A

R

S

E

K

V

G

G

I

A

E

D

L

A

V

A

Y

V

V

K

N
x
S

T

G

K

A

G

G

M

A

S

A

D

D

P

-

V

-

Q

-

M

A

L

I

R

R

A

E

L

L

T

T

G

G

D

G

A

Q

G

G

F

A

D

T

D

A

I

V

F

F

V

D

Y
x
F

A
x
V

A

G

V

A

P

Q

A

S

V
x
T

V

I

E

D

M

T

A

A

D

Q

E

Q

L

V

A

A

E

V

D

D

G

G

C

H

L

I

N

S

F

V

F
x
V

A
x
G

G
x
I

P

H

T

A

D

G

K

A

N

H

F

A

K

K

V

V

P

-

F

-

N

G

F

F

Y

F

N

M

V

I

H

P

Y

F

N

G

S

A

T

S

H

-

V

V

G

T

T

P

S
x
F

G

A

G

G

S

T

T

R

D

S

D

E

M

L

K

M

E

E

A

V

I

V

A

A

L

L

S

A

A

R

T

A

G

G

Q

R

L

L

active site: H62 (= H68), E63 (= E69), C92 (≠ A105), C95 (≠ S108), C98 (≠ Y111), C106 (≠ M119), A110 (≠ I126), D153 (≠ G153), T157 (≠ A157)
binding (2~{S})-2-methylpentanedioic acid: H62 (= H68), D153 (≠ G153), F294 (≠ S315)
binding nicotinamide-adenine-dinucleotide: D153 (≠ G153), T157 (≠ A157), V180 (≠ P186), G181 (≠ M187), G182 (= G188), L183 (≠ I189), D203 (= D209), L204 (≠ I210), R208 (= R214), S223 (≠ N238), F246 (≠ Y264), V247 (≠ A265), T252 (≠ V270), V269 (≠ F287), G270 (≠ A288), I271 (≠ G289), F294 (≠ S315)
binding zinc ion: H62 (= H68), C92 (≠ A105), C95 (≠ S108), C98 (≠ Y111), C106 (≠ M119), D153 (≠ G153)

Sites not aligning to the query:

active site: 38, 39, 40, 43, 340
binding (2~{S})-2-methylpentanedioic acid: 40
binding nicotinamide-adenine-dinucleotide: 38, 39, 40, 43, 340
binding zinc ion: 38

6ffxA Crystal structure of r. Ruber adh-a, mutant f43h (see paper)
24% identity, 64% coverage: 63:334/425 of query aligns to 57:313/346 of 6ffxA

query
sites
6ffxA

P

P

V

L

I

T

T

L

G

G

H
|
H

E

E

C

G

A

V

G

G

V

T

I

V

V

A

E

E

V

L

G

G

K

E

N

G

L

V

T

T

G

G

K

-

Y

-

K

-

G

-

Q

-

R

-

F

F

V

G

L

V

Q

G

P

D

A

A

M

V

G

A

L

V

P

Y

S

G

G

P

Y

W

S

G

A
x
C

G

G

Y

A

S
x
C

Y

H

E

A

Y
x
C

F

A

G

R

G

G

N

R

A

E

T

N

Y

Y

M
x
C

I

-

P

-

E

T

I

R

A

A

I

A

N

D

L

L

G

G

C

I

V

T

L

P

P

P

Y

G

H

L

G

G

S

S

Y

-

F

-

A

-

A

P

A

G

S

S

L

M

A

A

E

E

P

Y

M

M

C

I

C

V

-

D

-

S

-

A

-

R

-

H

-

L

-

V

-

P

-

I

-

G

-

D

-

L

-

D

-

P

-

V

-

A

-

P

I

L

I

T

G
x
D

A

A

Y

G

H

L

A
x
T

N

P

Y

Y

H

H

T

A

T

I

Q

S

Y

R

V

V

Y

L

E

P

H

L

R

-

M

-

G

-

V

L

K

G

P

P

G

G

G

S

N

T

I

A

A

V

L

V

L

I

A

-

C

-

A

-

G

G

P
x
V

M
x
G

G
|
G

I
x
L

G

G

A

H

I

V

D

G

Y

I

A

Q

I

I

N

L

G

R

G

A

I

V

Q

S

P

A

S

A

R

R

V

V

V

I

V

A

V

V

D
|
D

I
x
L

D

D

K

D

R
|
R

L

L

A

A

Q

-

V

-

Q

-

K

-

L

-

P

-

V

-

E

-

L

L

A

A

A

R

S

E

K

V

G

G

I

A

E

D

L

A

V

A

Y

V

V

K

N
x
S

T

G

K

A

G

G

M

A

S

A

D

D

P

-

V

-

Q

-

M

A

L

I

R

R

A

E

L

L

T

T

G

G

D

G

A

Q

G

G

F

A

D

T

D

A

I

V

F

F

V

D

Y
x
F

A
x
V

A

G

V

A

P

Q

A

S

V

T

V

I

E

D

M

T

A

A

D

Q

E

Q

L

V

A

A

E

V

D

D

G

G

C

H

L

I

N

S

F

V

F
x
V

A

G

G

I

P

H

T

A

D

G

K

A

N

H

F

A

K

K

V

V

P

-

F

-

N

G

F

F

Y

F

N

M

V

I

H

P

Y

F

N

G

S

A

T

S

H

-

V

V

G

T

T

P

S

Y

G

W

G

G

S

T

T

R

D

S

D

E

M

L

K

M

E

E

A

V

I

V

A

A

L

L

S

A

A

R

T

A

G

G

Q

R

L

L

active site: H62 (= H68), D153 (≠ G153)
binding nicotinamide-adenine-dinucleotide: D153 (≠ G153), T157 (≠ A157), V180 (≠ P186), G181 (≠ M187), G182 (= G188), L183 (≠ I189), D203 (= D209), L204 (≠ I210), R208 (= R214), S223 (≠ N238), F246 (≠ Y264), V247 (≠ A265), V269 (≠ F287)
binding zinc ion: H62 (= H68), C92 (≠ A105), C95 (≠ S108), C98 (≠ Y111), C106 (≠ M119), D153 (≠ G153)

Sites not aligning to the query:

active site: 38, 40, 43
binding nicotinamide-adenine-dinucleotide: 38, 39, 40, 43, 340
binding zinc ion: 38

6z42A The low resolution structure of a zinc-dependent alcohol dehydrogenase from halomonas elongata.
23% identity, 82% coverage: 1:349/425 of query aligns to 4:319/336 of 6z42A

query
sites
6z42A

M

M

K

K

T

S

K

A

V

V

A

V

A

R

I

T

Y

F

G

-

K

R

R

E

D

P

V

L

R

V

L

I

R

E

V

E

F

V

E

P

L

V

P

P

E

R

I

P

T

G

D

P

N

G

E

E

L

I

L

L

V

V

S

K

V

V

I

A

S

A

D

S

S

G

V

V

C
|
C

L

H

S
x
T

T

D

W

L

K
x
H

A

A

L

R

L

G

G

D

S

W

E

P

H

V

K

K

R

P

V

E

P

P

D

-

L

-

E

-

N

-

H

-

P

P

V

F

I

I

T

P

G

G

H
|
H

E
|
E

C

G

A

V

G

G

V

H

I

V

V

V

E

A

V

V

G

G

K

R

N

G

L

V

T

Q

G

-

K

H

Y

V

K

K

K

E

G

G

Q

D

R

R

F

-

V

-

L

-

Q

-

P

-

A

-

M

V

G

G

L

V

P

P

S

W

G

L

Y

Y

S

S

-

T

A
x
C

G

G

Y

H

S
x
C

Y

E

E

H

Y
x
C

F

L

G

G

-

W

-

E

-

T

-

L

-
x
C

-

E

-

S

-

Q

N

N

A

T

T

G

Y

Y

M

S

I

V

I

N

P

G

E

E

I

Y

A

T

I

L

N

A

L

Q

G

A

C

D

V

Y

L

V

P

G

Y

K

H

L

G

P

S

D

Y

N

F

V

A

G

A

F

A

V

S

E

L

I

A

A

E

P

P

V

M

L

C
|
C

C

A

I

G

I

V

G

T

A

V

Y

Y

H

K

A

G

N

L

Y

K

H

M

T

T

T

D

Q

-

Y

-

V

-

Y

-

E

-

H

-

R

-

M

-

G

-

V

T

K

R

P

P

G

G

G

Q

N

W

I

V

A

V

L

I

L

S

A

G

C

I

A

G

G

G

P

-

M

L

G

G

I

H

G

M

A

A

I

V

D

Q

Y

Y

A

A

I

R

N

A

G

M

G

G

I

L

Q

N

P

-

S

-

R

-

V

V

V

A

V

V

D

D

I

V

D

D

K

A

R

K

L

L

A

A

Q

L

V

A

Q

E

K

R

L

L

L

G

P

A

V

T

E

-

L

-

A

-

S

-

K

-

G

-

I

-

E

-

L

-

V

I

Y

T

V

V

N

N

T

A

K

M

G

-

M

Q

S

T

D

D

P

P

V

A

Q

R

M

Y

L

L

R

K

A

Q

L

T

T

I

G

G

D

-

A

-

G

G

F

A

D

H

D

G

I

A

F

L

V

V

Y

T

A

A

A

V

V

S

P

P

A

K

V

A

V

F

E

E

M

Q

A

A

D

L

E

G

L

M

L

I

A

R

E

R

D

G

G

G

C

T

L

V

N

A

F

L

F

N

A

G

G

L

P

P

T

P

D

G

K

-

N

-

F

-

K

D

V

F

P

P

F

L

N

P

F

I

Y

F

N

D

V

M

H

V

Y

L

N

N

S

G

T

I

H

T

V

V

V

R

G

G

T

S

S

I

G

V

G

G

S

T

T

R

D

L

D

D

M

L

K

Q

E

E

A

A

I

L

A

D

L

F

S

A

A

G

T

E

G

G

Q

K

L

V

Q

Q

P

A

S

T

F

V

M

K

V

T

T

E

H

K

I

L

G

E

G

N

L

I

D

N

A

T

V

V

active site: C41 (= C39), T43 (≠ S41), H46 (≠ K44), H64 (= H68), C148 (= C149)
binding zinc ion: C41 (= C39), H64 (= H68), E65 (= E69), C95 (≠ A105), C98 (≠ S108), C101 (≠ Y111), C109 (vs. gap), C148 (= C149)

1pl6A Human sdh/nadh/inhibitor complex (see paper)
23% identity, 59% coverage: 8:257/425 of query aligns to 13:240/356 of 1pl6A

query
sites
1pl6A

I

V

Y

H

G

G

K

P

R

G

D

D

V

L

R

R

L

L

R

E

V

N

F

Y

E

P

L

I

P

P

E

E

I

P

T

G

D

P

N

N

E

E

L

V

L

L

V

L

S

R

V

M

I

H

S

S

D

V

S

G

V

I

C
|
C

L
x
G

S
|
S

T

D

-

V

-
x
H

-

Y

W

W

K

-

A

-

L

-

G

-

S

-

E

E

H

Y

K

G

R

R

V
x
I

P

G

D

N

D
x
F

L

I

E

V

N

K

H

K

P

P

V

M

I

V

T

L

G

G

H
|
H

E
|
E

C

A

A

S

G

G

V

T

I

V

V

E

E

K

V

V

G

G

K

S

N

S

L

V

T

K

G

-

K

H

Y

L

K

K

K

P

G

G

Q

D

R

R

F

V

V

A

L

I

Q

E

P

P

A

G

M

-

G

-

L

-

P

-

S

-

G

-

Y

-

S

-

A

A

G

P

Y
x
R

S

E

Y

N

E
x
D

Y

E

F

F

G
x
C

G

K

N

M

A

G

T

R

Y

Y

M

N

I

L

I
x
S

P

P

E

S

I

I

A
x
F

I

F

-

C

-

A

-

T

-

P

-

D

-

G

N

N

L

L

G

C

C

R

V

F

L

Y

P

K

Y

H

H

N

G

A

S

A

Y

F

-

C

-

Y

-

K

-

L

-

P

-

D

-

N

-

V

-

T

F

F

A

E

A

G

S

A

L

L

A

I

E
|
E

P
|
P

M

L

C

S

C

V

I
x
G

I

I

G

H

A

A

Y

C

H

-

A

-

N

-

Y

-

H

-

T

-

Q

-

Y

-

V

-

Y

-

E

-

H

R

R

R

M

G

G

G

V

V

K

T

P

L

G

G

G

H

N

K

I

V

A

-

L

L

L

V

A

C

C

G

A

A

G
|
G

P
|
P

M

-

G

-

I
|
I

G

G

A

M

I

V

D

T

Y

L

A

L

I

V

N

A

G

K

G

A

I

M

Q

G

P

A

S

A

R

Q

V

V

V

T

D
|
D

I
x
L

D

S

D

A

K

T

R
|
R

L

L

A

S

Q

K

V

A

Q

K

K

E

L

I

L

-

P

-

V

-

E

-

L

-

A

-

S

-

K

-

G

G

I

A

E

D

L

L

V

V

Y

L

V

Q

N

I

T

S

K

K

G

-

M

-

S

E

D

S

P

P

V

Q

Q

E

M

I

L

A

R

R

A

K

L

V

T

E

G

G

D

Q

A

L

G

G

active site: C44 (= C39), G45 (≠ L40), S46 (= S41), H49 (vs. gap), H69 (= H68), E70 (= E69), R99 (≠ Y107), D102 (≠ E110), C105 (≠ G113), S113 (≠ I121), F117 (≠ A125), P156 (= P147), G160 (≠ I151)
binding 4-[2-(hydroxymethyl)pyrimidin-4-yl]-n,n-dimethylpiperazine-1-sulfonamide: C44 (= C39), S46 (= S41), I56 (≠ V55), F59 (≠ D58), H69 (= H68), E155 (= E146)
binding nicotinamide-adenine-dinucleotide: G181 (= G185), P182 (= P186), I183 (= I189), D203 (= D209), L204 (≠ I210), R208 (= R214)
binding zinc ion: C44 (= C39), H69 (= H68)

Sites not aligning to the query:

active site: 344
binding 4-[2-(hydroxymethyl)pyrimidin-4-yl]-n,n-dimethylpiperazine-1-sulfonamide: 274, 297
binding nicotinamide-adenine-dinucleotide: 249, 250, 272, 273, 274, 297, 298

Q00796 Sorbitol dehydrogenase; SDH; (R,R)-butanediol dehydrogenase; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Ribitol dehydrogenase; RDH; Xylitol dehydrogenase; XDH; EC 1.1.1.-; EC 1.1.1.4; EC 1.1.1.14; EC 1.1.1.56; EC 1.1.1.9 from Homo sapiens (Human) (see 10 papers)
23% identity, 59% coverage: 8:257/425 of query aligns to 14:241/357 of Q00796

query
sites
Q00796

I

V

Y

H

G

G

K

P

R

G

D

D

V

L

R

R

L

L

R

E

V

N

F

Y

E

P

L

I

P

P

E

E

I

P

T

G

D

P

N

N

E

E

L

V

L

L

V

L

S

R

V

M

I

H

S

S

D

V

S

G

V

I

C
|
C

L

G

S

S

T

D

-

V

-

H

-

Y

W

W

K

-

A

-

L

-

G

-

S

-

E

E

H

Y

K

G

R

R

V

I

P

G

D

N

D

F

L

I

E

V

N

K

H

K

P

P

V

M

I

V

T

L

G

G

H
|
H

E
|
E

C

A

A

S

G

G

V

T

I

V

V

E

E

K

V

V

G

G

K

S

N

S

L

V

T

K

G

-

K

H

Y

L

K

K

K

P

G

G

Q

D

R

R

F

V

V

A

L

I

Q

E

P

P

A

G

M

-

G

-

L

-

P

-

S

-

G

-

Y

-

S

-

A

A

G

P

Y

R

S

E

Y

N

E

D

Y

E

F

F

G

C

G

K

N

M

A

G

T
x
R

Y

Y

M

N

I

L

I

S

P

P

E

S

I

I

A

F

I

F

-

C

-

A

-

T

-

P

-

D

-

G

N

N

L

L

G

C

C

R

V

F

L

Y

P

K

Y
x
H

H

N

G

A

S

A

Y

F

-

C

-

Y

-

K

-

L

-

P

-

D

-

N

-

V

-

T

F

F

A

E

A

G

S
x
A

L

L

A

I

E

E

P

P

M

L

C

S

C

V

I

G

I

I

G

H

A

A

Y

C

H

-

A

-

N

-

Y

-

H

-

T

-

Q

-

Y

-

V

-

Y

-

E

-

H

R

R

R

M

G

G

G

V

V

K

T

P

L

G

G

G

H

N

K

I

V

A

-

L

L

L

V

A

C

C

G

A

A

G

G

P

P

M

-

G

-

I
|
I

G

G

A

M

I

V

D

T

Y

L

A

L

I

V

N

A

G

K

G

A

I

M

Q

G

P

A

S

A

R

Q

V

V

V

T

D
|
D

I

L

D

S

D

A

K

T

R
|
R

L

L

A

S

Q

K

V

A

Q

K

K

E

L

I

L

-

P

-

V

-

E

-

L

-

A

-

S

-

K

-

G

G

I

A

E

D

L

L

V

V

Y

L

V

Q

N

I

T

S

K

K

G

-

M

-

S

E

D

S

P

P

V

Q

Q

E

M

I

L

A

R

R

A

K

L

V

T

E

G

G

D
x
Q

A

L

G

G

C45 (= C39) binding
H70 (= H68) binding
E71 (= E69) binding
R110 (≠ T117) to P: in SORDD; results in protein aggregation
H135 (≠ Y134) to R: in SORDD; results in protein aggregation
A153 (≠ S143) to D: in SORDD; uncertain significance; results in protein aggregation; dbSNP:rs145813597
I184 (= I189) binding
D204 (= D209) binding
R209 (= R214) binding
Q239 (≠ D255) to L: in dbSNP:rs1042079

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
269 N → T: in dbSNP:rs930337
273:275 binding
297:299 binding
322 V → I: in SORDD; uncertain significance; dbSNP:rs149975952

7y9pA Xylitol dehydrogenase s96c/s99c/y102c mutant(thermostabilized form) from pichia stipitis (see paper)
24% identity, 49% coverage: 13:221/425 of query aligns to 13:214/357 of 7y9pA

query
sites
7y9pA

D

D

V

I

R

S

L

F

R

E

V

T

F

Y

E

D

L

A

P

P

E

E

I

I

T

S

D

E

-

P

N

T

E

D

L

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L

L

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V

V

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S

K

D

T

S

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C
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C

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G

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D

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I

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H

A

F

A

-

L

-

G

-

S

Y

E

A

H

H

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R

R

V

I

P

G

D

N

D

F

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E

L

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M

I

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G

H
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H

E
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E

C

S

A

A

G

G

V

T

I

V

V

V

E

Q

V

V

G

G

K

K

N

G

L

V

T

T

G

-

K

S

Y

L

K

K

K

V

G

G

Q

D

R

N

F

V

V

A

L

I

Q

E

P

P

A

-

M

-

G

G

L

I

P

P

S
x
C

G

R

Y

F

S
x
C

A

D

G

E

Y
x
C

S

K

Y

S

E

G

Y

H

F

Y

-

N

-

L

-
x
C

-

P

-

H

-

M

-

A

-

F

-

A

-

T

-

P

-

N

-

E

-

P

-

N

-

P

G

G

G

T

N

L

A

C

T

K

Y

Y

M

F

I

K

I

S

P

P

E

E

I

-

A

-

I

-

N

D

L

F

G

L

C

V

V

K

L

L

P

P

Y

D

H

H

G

V

S

S

Y

-

F

L

A

E

A

L

A

G

S

A

L

L

A

V

E

E

P

P

M

L

C

-

I

-

G

-

A

-

Y

-

H

-

A

-

N

-

Y

-

H

-

T

-

T

S

Q

V

Y

G

V

V

Y

H

E

A

H

S

R

K

M

L

G

G

V

S

K

V

P

A

G

F

G

G

N

D

I

Y

A

V

L

A

L

V

A

F

C

G

A

A

G

G

P

P

M

V

G

G

I

L

G

L

A

A

I

A

D

A

Y

V

A

A

I

K

N

T

G

F

G

G

I

A

Q

K

P

-

S

-

R

G

V

V

V

I

V

V

D

D

I

I

D

F

D

D

K

N

R

K

L

L

A

K

Q

M

V

A

Q

K

K

D

L

I

binding zinc ion: C40 (= C39), H65 (= H68), E66 (= E69), C95 (≠ S101), C98 (≠ S104), C101 (≠ Y107), C109 (vs. gap)

1r37A Alcohol dehydrogenase from sulfolobus solfataricus complexed with NAD(h) and 2-ethoxyethanol (see paper)
22% identity, 82% coverage: 12:358/425 of query aligns to 11:336/347 of 1r37A

query
sites
1r37A

R

K

D

P

V

L

R

S

L

L

R

Q

V

E

F

I

E

G

L

V

P

P

E

K

I

P

T

K

D

G

N

P

E

Q

L

V

L

L

V

I

S

K

V

V

I

E

S

A

D

A

S

G

V

V

C
|
C

L
x
H

S
|
S

T

D

W

V

K
x
H

A

M

A

R

L

Q

L

G

G

R

S
x
F

E

G

H

N

K

L

R

R

V

I

P

V

D

E

D

D

L

L

E

G

-

V

N

K

H

L

P

P

V

V

I

T

T

L

G

G

H
|
H

E
|
E

C

I

A

A

G

G

V

K

I

I

V

E

E

E

V

V

G

G

K

D

N

E

L

V

T

V

G

G

K

-

Y

Y

K

S

K

K

G

G

Q

D

R

L

F

V

V

A

L

V

Q

N

P

P

A

W

M

Q

G

G

L
x
E

P

G

S

N

G
x
C

Y

Y

S

Y

A
x
C

G

R

Y

I

S

G

Y

E

E

E

Y

H

F

L

G
x
C

G

D

N

S

A

P

T
x
R

Y

W

M

L

I

G

I

I

P

N

-

F

E

D

I

G

A

A

I

Y

N

A

L

E

G

Y

C

V

V

I

L

V

P

P

Y

H

H

Y

G

K

S

Y

Y

M

F

Y

A

K

A

L

A

R

S

R

L

L

-

N

-

A

-

V

-

E

A

A

E

A

P

P

M

L

C

T

C
|
C

I

-

G

-

A

-

Y

-

H

-

A

-

N

-

Y

S

H

G

T

I

T
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T

Q

T

Y

Y

V

R

Y

A

E

V

H

R

R

K

M

A

G

S

V

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P

P

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G

K

N

T

I

L

A

L

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V

A

G

C

A

A

G

G
|
G

P
x
G

M
x
L

G

G

I

T

G

M

A

A

I

V

D

Q

Y

I

A

A

I

-

N

-

G

K

G

A

I

V

Q

S

P

G

S

A

R

T

V

I

V

G

V

V

D
|
D

I
x
V

D
x
R

D

E

K

E

R

-

L

-

A

-

Q

-

V

-

Q

-

K

-

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-

P

A

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V

E

E

L

A

A

A

A

K

S

R

K

A

G

G

I

A

E

D

L

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-

Y

Y

V

V

N

I

T

N

K

A

G

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M

M

S

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D

P

P

V

L

Q

A

M

E

L

I

R

R

A

R

L

I

T

T

G

E

D

S

A

K

G

G

F

V

D

D

D

A

I

V

F

I

V

D

Y
x
L

A
x
N

A

N

V

S

P

E

A

K

V

T

V

L

E

S

M

V

A

Y

D

P

E

K

L

A

L

L

A

A

E

K

D

Q

G

G

-

K

-

Y

-

V

C

M

L
x
V

N
x
G

F
x
L

F
|
F

A

G

G

A

P

-

T

-

D

D

K

L

N

H

F

Y

K

H

V

A

P

P

F

L

N

-

F

-

Y

-

N

-

V

I

H

T

Y

L

N

S

S

E

T

I

H

Q

V

F

V

V

G

G

T

S

S
x
L

G
x
V

G

G

S

N

T

Q

D

S

D

D

M

F

K

L

E

G

A

I

I

M

A

R

L

L

S

A

A

E

T

A

G

G

Q

K

L

V

Q

K

P

P

S

-

F

-

M

M

V

I

T

T

H

K

I

T

G

M

G

K

L

L

D

E

A

E

V

A

P

N

E

E

T

A

V

I

L

D

N

N

L
|
L

P

E

D

N

active site: C38 (= C39), H39 (≠ L40), S40 (= S41), H43 (≠ K44), H68 (= H68), E69 (= E69), E98 (≠ L99), C101 (≠ G102), C104 (≠ A105), C112 (≠ G113), R116 (≠ T117), C154 (= C150), T158 (= T162)
binding 2-ethoxyethanol: S40 (= S41), F49 (≠ S50)
binding nicotinamide-adenine-dinucleotide: C38 (= C39), H39 (≠ L40), S40 (= S41), H43 (≠ K44), C154 (= C150), T158 (= T162), G181 (= G185), G182 (≠ P186), L183 (≠ M187), D203 (= D209), V204 (≠ I210), R205 (≠ D211), L247 (≠ Y264), N248 (≠ A265), V270 (≠ L284), G271 (≠ N285), L272 (≠ F286), F273 (= F287), L295 (≠ S315), V296 (≠ G316), L334 (= L356)
binding zinc ion: C38 (= C39), H68 (= H68), E98 (≠ L99), C101 (≠ G102), C104 (≠ A105), C112 (≠ G113), C154 (= C150)

Sites not aligning to the query:

active site: 342
binding nicotinamide-adenine-dinucleotide: 337, 342

1jvbA Alcohol dehydrogenase from the archaeon sulfolobus solfataricus (see paper)
22% identity, 82% coverage: 12:358/425 of query aligns to 11:336/347 of 1jvbA

query
sites
1jvbA

R

K

D

P

V

L

R

S

L

L

R

Q

V

E

F

I

E

G

L

V

P

P

E

K

I

P

T

K

D

G

N

P

E

Q

L

V

L

L

V

I

S

K

V

V

I

E

S

A

D

A

S

G

V

V

C
|
C

L
x
H

S
|
S

T

D

W

V

K
x
H

A

M

A

R

L

Q

L

G

G

R

S

F

E

G

H

N

K

L

R

R

V

I

P

V

D

E

D

D

L

L

E

G

-

V

N

K

H

L

P

P

V

V

I

T

T

L

G

G

H
|
H

E
|
E

C

I

A

A

G

G

V

K

I

I

V

E

E

E

V

V

G

G

K

D

N

E

L

V

T

V

G

G

K

-

Y

Y

K

S

K

K

G

G

Q

D

R

L

F

V

V

A

L

V

Q

N

P

P

A

W

M

Q

G

G

L
x
E

P

G

S

N

G
x
C

Y

Y

S

Y

A
x
C

G

R

Y

I

S

G

Y

E

E

E

Y

H

F

L

G
x
C

G

D

N

S

A

P

T
x
R

Y

W

M

L

I

G

I

I

P

N

-

F

E

D

I

G

A

A

I

Y

N

A

L

E

G

Y

C

V

V

I

L

V

P

P

Y

H

H

Y

G

K

S

Y

Y

M

F

Y

A

K

A

L

A

R

S

R

L

L

-

N

-

A

-

V

-

E

A

A

E

A

P

P

M

L

C

T

C
|
C

I

-

G

-

A

-

Y

-

H

-

A

-

N

-

Y

S

H

G

T

I

T
|
T

Q

T

Y

Y

V

R

Y

A

E

V

H

R

R

K

M

A

G

S

V

L

K

D

P

P

G

T

G

K

N

T

I

L

A

L

L

V

A

G

C

A

A

G

G

G

P

G

M

L

G

G

I

T

G

M

A

A

I

V

D

Q

Y

I

A

A

I

-

N

-

G

K

G

A

I

V

Q

S

P

G

S

A

R

T

V

I

V

G

V

V

D

D

I

V

D

R

D

E

K

E

R

-

L

-

A

-

Q

-

V

-

Q

-

K

-

L

-

P

A

V

V

E

E

L

A

A

A

A

K

S

R

K

A

G

G

I

A

E

D

L

-

V

-

Y

Y

V

V

N

I

T

N

K

A

G

S

M

M

S

Q

D

D

P

P

V

L

Q

A

M

E

L

I

R

R

A

R

L

I

T

T

G

E

D

S

A

K

G

G

F

V

D

D

D

A

I

V

F

I

V

D

Y

L

A

N

V

S

P

E

A

K

V

T

V

L

E

S

M

V

A

Y

D

P

E

K

L

A

L

L

A

A

E

K

D

Q

G

G

-

K

-

Y

-

V

C

M

L

V

N

G

F

L

F

F

A

G

G

A

P

-

T

-

D

D

K

L

N

H

F

Y

K

H

V

A

P

P

F

L

N

-

F

-

Y

-

N

-

V

I

H

T

Y

L

N

S

S

E

T

I

H

Q

V

F

V

V

G

G

T

S

S

L

G

V

G

G

S

N

T

Q

D

S

D

D

M

F

K

L

E

G

A

I

I

M

A

R

L

L

S

A

A

E

T

A

G

G

Q

K

L

V

Q

K

P

P

S

-

F

-

M

M

V

I

T

T

H

K

I

T

G

M

G

K

L

L

D

E

A

E

V

A

P

N

E

E

T

A

V

I

L

D

N

N

L

L

P

E

D

N

active site: C38 (= C39), H39 (≠ L40), S40 (= S41), H43 (≠ K44), H68 (= H68), E69 (= E69), E98 (≠ L99), C101 (≠ G102), C104 (≠ A105), C112 (≠ G113), R116 (≠ T117), C154 (= C150), T158 (= T162)
binding zinc ion: C38 (= C39), H68 (= H68), E69 (= E69), E98 (≠ L99), C101 (≠ G102), C104 (≠ A105), C112 (≠ G113), C154 (= C150)

Sites not aligning to the query:

active site: 342

Query Sequence

>1936889 FitnessBrowser__Keio:1936889
MKTKVAAIYGKRDVRLRVFELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLE
NHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNIAL
LACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVELAASKGIELVYVNTK
GMSDPVQMLRALTGDAGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGPTDKNFKVPFN
FYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPETVLNLPDIP
GGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEKYLLAQFGVDI
GEAAQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory