SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 1936903 FitnessBrowser__Keio:1936903 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

Q9YHT2 Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; Iron-sulfur subunit of complex II; Ip; EC 1.3.5.1 from Gallus gallus (Chicken) (see 2 papers)
30% identity, 21% coverage: 22:105/407 of query aligns to 193:277/290 of Q9YHT2

query
sites
Q9YHT2

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C196 (= C25) binding
C199 (= C28) binding
C202 (= C31) binding
C206 (= C35) binding
W211 (≠ L40) binding
C253 (= C75) binding
C259 (= C81) binding
C263 (= C85) binding

Sites not aligning to the query:

103 binding
108 binding
111 binding
123 binding

Q007T0 Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; Iron-sulfur subunit of complex II; Ip; EC 1.3.5.1 from Sus scrofa (Pig) (see paper)
30% identity, 21% coverage: 22:105/407 of query aligns to 183:267/280 of Q007T0

query
sites
Q007T0

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C186 (= C25) binding
C189 (= C28) binding
C192 (= C31) binding
C196 (= C35) binding
W201 (≠ L40) binding
C243 (= C75) binding
C249 (= C81) binding
C253 (= C85) binding

Sites not aligning to the query:

93 binding
98 binding
101 binding
113 binding

Query Sequence

>1936903 FitnessBrowser__Keio:1936903
MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLE
GNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIVEQKVKRPLPERILREGLR
QVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKPRPPLRHKRRVLMLEGCAQPT
LSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNAQEKGLARARNNIDAWWPAIEAG
AEAILQTASGCGAFVKEYGQMLKNDALYADKARQVSELAVDLVELLREEPLEKLAIRGDK
KLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDVPDSHLCCGSAGTYALTHPDLARQLRD
NKMNALESGKPEMIVTANIGCQTHLASAGRTSVRHWIEIVEQALEKE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory