SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 1937119 FitnessBrowser__Keio:1937119 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
100% identity, 98% coverage: 5:308/309 of query aligns to 1:304/304 of 1iyeA

query
sites
1iyeA

K

K

A

A

D

D

Y

Y

I

I

W

W

F

F

N

N

G

G

E

E

M

M

V

V

R

R

W

W

E

E

D

D

A

A

K

K

V

V

H

H

V

V

M

M

S

S

H

H

A

A

L

L

H

H

Y

Y

G

G

T

T

S

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

C

C

Y

Y

D

D

S

S

H

H

K

K

G

G

P

P

V

V

V

V

F

F

R

R

H

H

R

R

E

E

H

H

M

M

Q

Q

R
|
R

L

L

H

H

D

D

S

S

A

A

K

K

I

I

Y

Y

R

R

F

F

P

P

V

V

S

S

Q

Q

S

S

I

I

D

D

E

E

L

L

M

M

E

E

A

A

C

C

R

R

D

D

V

V

I

I

R

R

K

K

N

N

N

N

L

L

T

T

S

S

A

A

Y
|
Y

I

I

R

R

P

P

L

L

I

I

F

F

V

V

G

G

D

D

V

V

G

G

M

M

G

G

V

V

N

N

P

P

P

P

A

A

G

G

Y

Y

S

S

T

T

D

D

V

V

I

I

I

I

A

A

A

A

F

F

P

P

W

W

G

G

A

A

Y
|
Y

L

L

G

G

A

A

E

E

A

A

L

L

E

E

Q

Q

G

G

I

I

D

D

A

A

M

M

V

V

S

S

S

S

W

W

N

N

R

R

A

A

A

A

P

P

N

N

T

T

I

I

P

P

T

T

A

A

A

A

K
|
K

A
|
A

G

G

G

G

N

N

Y
|
Y

L

L

S

S

S

S

L

L

L

L

V

V

G

G

S

S

E

E

A

A

R

R

R

R

H

H

G

G

Y

Y

Q

Q

E

E

G

G

I

I

A

A

L

L

D

D

V

V

N

N

G

G

Y

Y

I

I

S

S

E
|
E

G

G

A

A

G
|
G

E
|
E

N
|
N

L

L

F

F

E

E

V

V

K

K

D

D

G

G

V

V

L

L

F

F

T

T

P

P

P

P

F

F

T

T

S

S

S

S

A

A

L
|
L

P

P

G
|
G

I
|
I

T
|
T

R

R

D

D

A

A

I

I

I

I

K

K

L

L

A

A

K

K

E

E

L

L

G

G

I

I

E

E

V

V

R

R

E

E

Q

Q

V

V

L

L

S

S

R

R

E

E

S

S

L

L

Y

Y

L

L

A

A

D

D

E

E

V

V

F

F

M

M

S

S

G
|
G

T
|
T

A
|
A

A

A

E

E

I

I

T

T

P

P

V

V

R

R

S

S

V

V

D

D

G

G

I

I

Q

Q

V

V

G

G

E

E

G

G

R

R

C

C

G

G

P

P

V

V

T

T

K

K

R

R

I

I

Q

Q

Q

Q

A

A

F

F

F

F

G

G

L

L

F

F

T

T

G

G

E

E

T

T

E

E

D

D

K

K

W

W

G

G

W

W

L

L

D

D

Q

Q

V

V

N

N

active site: F33 (= F37), G35 (= G39), K156 (= K160), A157 (= A161), E190 (= E194), L214 (= L218)
binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R60), Y92 (= Y96), Y126 (= Y130), K156 (= K160), Y161 (= Y165), E190 (= E194), G193 (= G197), E194 (= E198), N195 (= N199), L214 (= L218), G216 (= G220), I217 (= I221), T218 (= T222), G253 (= G257), T254 (= T258), A255 (= A259)

1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
100% identity, 98% coverage: 5:308/309 of query aligns to 1:304/304 of 1iydA

query
sites
1iydA

K

K

A

A

D

D

Y

Y

I

I

W

W

F

F

N

N

G

G

E

E

M

M

V

V

R

R

W

W

E

E

D

D

A

A

K

K

V

V

H

H

V

V

M

M

S

S

H

H

A

A

L

L

H

H

Y

Y

G

G

T

T

S

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

C

C

Y

Y

D

D

S

S

H

H

K

K

G

G

P

P

V

V

V

V

F

F

R

R

H

H

R

R

E

E

H

H

M

M

Q

Q

R
|
R

L

L

H

H

D

D

S

S

A

A

K

K

I

I

Y

Y

R

R

F

F

P

P

V

V

S

S

Q

Q

S

S

I

I

D

D

E

E

L

L

M

M

E

E

A

A

C

C

R

R

D

D

V

V

I

I

R

R

K

K

N

N

N

N

L

L

T

T

S

S

A

A

Y
|
Y

I

I

R

R

P

P

L

L

I

I

F

F

V

V

G

G

D

D

V

V

G

G

M

M

G

G

V

V

N

N

P

P

P

P

A

A

G

G

Y

Y

S

S

T

T

D

D

V

V

I

I

I

I

A

A

A

A

F

F

P

P

W

W

G

G

A

A

Y
|
Y

L

L

G

G

A

A

E

E

A

A

L

L

E

E

Q

Q

G

G

I

I

D

D

A

A

M

M

V

V

S

S

S

S

W

W

N

N

R

R

A

A

A

A

P

P

N

N

T

T

I

I

P

P

T

T

A

A

A

A

K
|
K

A
|
A

G

G

G

G

N

N

Y
|
Y

L

L

S

S

S

S

L

L

L

L

V

V

G

G

S

S

E

E

A

A

R

R

R

R

H

H

G

G

Y

Y

Q

Q

E

E

G

G

I

I

A

A

L

L

D

D

V

V

N

N

G

G

Y

Y

I

I

S

S

E
|
E

G

G

A

A

G
|
G

E
|
E

N

N

L

L

F

F

E

E

V

V

K

K

D

D

G

G

V

V

L

L

F

F

T

T

P

P

P

P

F

F

T

T

S

S

S

S

A

A

L
|
L

P

P

G
|
G

I
|
I

T
|
T

R

R

D

D

A

A

I

I

I

I

K

K

L

L

A

A

K

K

E

E

L

L

G

G

I

I

E

E

V

V

R

R

E

E

Q

Q

V

V

L

L

S

S

R

R

E

E

S

S

L

L

Y

Y

L

L

A

A

D

D

E

E

V

V

F

F

M

M

S

S

G

G

T
|
T

A
|
A

A

A

E

E

I

I

T

T

P

P

V

V

R

R

S

S

V

V

D

D

G

G

I

I

Q

Q

V

V

G

G

E

E

G

G

R

R

C

C

G

G

P

P

V

V

T

T

K

K

R

R

I

I

Q

Q

Q

Q

A

A

F

F

F

F

G

G

L

L

F

F

T

T

G

G

E

E

T

T

E

E

D

D

K

K

W

W

G

G

W

W

L

L

D

D

Q

Q

V

V

N

N

active site: F33 (= F37), G35 (= G39), K156 (= K160), A157 (= A161), E190 (= E194), L214 (= L218)
binding glutaric acid: Y92 (= Y96), Y126 (= Y130), A255 (= A259)
binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K160), Y161 (= Y165), E190 (= E194), G193 (= G197), E194 (= E198), L214 (= L218), G216 (= G220), I217 (= I221), T218 (= T222), T254 (= T258)

1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
100% identity, 98% coverage: 5:308/309 of query aligns to 1:304/304 of 1i1mA

query
sites
1i1mA

K

K

A

A

D

D

Y

Y

I

I

W

W

F

F

N

N

G

G

E

E

M

M

V

V

R

R

W

W

E

E

D

D

A

A

K

K

V

V

H

H

V

V

M

M

S

S

H

H

A

A

L

L

H

H

Y

Y

G

G

T

T

S

S

V

V

F

F

E

E

G

G

I

I

R

R

C

C

Y

Y

D

D

S

S

H

H

K

K

G

G

P

P

V

V

V

V

F

F

R

R

H

H

R

R

E

E

H

H

M

M

Q

Q

R
|
R

L

L

H

H

D

D

S

S

A

A

K

K

I

I

Y

Y

R

R

F

F

P

P

V

V

S

S

Q

Q

S

S

I

I

D

D

E

E

L

L

M

M

E

E

A

A

C

C

R

R

D

D

V

V

I

I

R

R

K

K

N

N

N

N

L

L

T

T

S

S

A

A

Y
|
Y

I

I

R

R

P

P

L

L

I

I

F

F

V

V

G

G

D

D

V

V

G

G

M

M

G

G

V

V

N

N

P

P

P

P

A

A

G

G

Y

Y

S

S

T

T

D

D

V

V

I

I

I

I

A

A

A

A

F

F

P

P

W

W

G

G

A

A

Y

Y

L

L

G

G

A

A

E

E

A

A

L

L

E

E

Q

Q

G

G

I

I

D

D

A

A

M

M

V

V

S

S

S

S

W

W

N

N

R

R

A

A

A

A

P

P

N

N

T

T

I

I

P

P

T

T

A

A

A

A

K
|
K

A

A

G

G

G

G

N

N

Y
|
Y

L

L

S

S

S

S

L

L

L

L

V

V

G

G

S

S

E

E

A

A

R

R

R

R

H

H

G

G

Y

Y

Q

Q

E

E

G

G

I

I

A

A

L

L

D

D

V

V

N

N

G

G

Y

Y

I

I

S

S

E
|
E

G

G

A

A

G
|
G

E
|
E

N

N

L

L

F

F

E

E

V

V

K

K

D

D

G

G

V

V

L

L

F

F

T

T

P

P

P

P

F

F

T

T

S

S

S

S

A

A

L
|
L

P

P

G
|
G

I
|
I

T
|
T

R

R

D

D

A

A

I

I

I

I

K

K

L

L

A

A

K

K

E

E

L

L

G

G

I

I

E

E

V

V

R

R

E

E

Q

Q

V

V

L

L

S

S

R

R

E

E

S

S

L

L

Y

Y

L

L

A

A

D

D

E

E

V

V

F

F

M

M

S

S

G
|
G

T
|
T

A
|
A

A

A

E

E

I

I

T

T

P

P

V

V

R

R

S

S

V

V

D

D

G

G

I

I

Q

Q

V

V

G

G

E

E

G

G

R

R

C

C

G

G

P

P

V

V

T

T

K

K

R

R

I

I

Q

Q

Q

Q

A

A

F

F

F

F

G

G

L

L

F

F

T

T

G

G

E

E

T

T

E

E

D

D

K

K

W

W

G

G

W

W

L

L

D

D

Q

Q

V

V

N

N

active site: K156 (= K160)
binding 4-methyl valeric acid: Y92 (= Y96), K156 (= K160), T254 (= T258), A255 (= A259)
binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K160), Y161 (= Y165), E190 (= E194), G193 (= G197), E194 (= E198), L214 (= L218), G216 (= G220), I217 (= I221), T218 (= T222), G253 (= G257), T254 (= T258)

1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
100% identity, 98% coverage: 5:308/309 of query aligns to 1:304/304 of 1i1lA

query
sites
1i1lA

K

K

A

A

D

D

Y

Y

I

I

W

W

F

F

N

N

G

G

E

E

M

M

V

V

R

R

W

W

E

E

D

D

A

A

K

K

V

V

H

H

V

V

M

M

S

S

H

H

A

A

L

L

H

H

Y

Y

G

G

T

T

S

S

V

V

F

F

E

E

G

G

I

I

R

R

C

C

Y

Y

D

D

S

S

H

H

K

K

G

G

P

P

V

V

V

V

F

F

R

R

H

H

R

R

E

E

H

H

M

M

Q

Q

R
|
R

L

L

H

H

D

D

S

S

A

A

K

K

I

I

Y

Y

R

R

F

F

P

P

V

V

S

S

Q

Q

S

S

I

I

D

D

E

E

L

L

M

M

E

E

A

A

C

C

R

R

D

D

V

V

I

I

R

R

K

K

N

N

N

N

L

L

T

T

S

S

A

A

Y
|
Y

I

I

R

R

P

P

L

L

I

I

F

F

V

V

G

G

D

D

V

V

G

G

M

M

G

G

V

V

N

N

P

P

P

P

A

A

G

G

Y

Y

S

S

T

T

D

D

V

V

I

I

I

I

A

A

A

A

F

F

P

P

W

W

G

G

A

A

Y

Y

L

L

G

G

A

A

E

E

A

A

L

L

E

E

Q

Q

G

G

I

I

D

D

A

A

M

M

V

V

S

S

S

S

W

W

N

N

R

R

A

A

A

A

P

P

N

N

T

T

I

I

P

P

T

T

A

A

A

A

K
|
K

A

A

G

G

G

G

N

N

Y
|
Y

L

L

S

S

S

S

L

L

L

L

V

V

G

G

S

S

E

E

A

A

R

R

R

R

H

H

G

G

Y

Y

Q

Q

E

E

G

G

I

I

A

A

L

L

D

D

V

V

N

N

G

G

Y

Y

I

I

S

S

E
|
E

G

G

A

A

G
|
G

E

E

N

N

L

L

F

F

E

E

V

V

K

K

D

D

G

G

V

V

L

L

F

F

T

T

P

P

P

P

F

F

T

T

S

S

S

S

A

A

L

L

P

P

G
|
G

I
|
I

T
|
T

R

R

D

D

A

A

I

I

I

I

K

K

L

L

A

A

K

K

E

E

L

L

G

G

I

I

E

E

V

V

R

R

E

E

Q

Q

V

V

L

L

S

S

R

R

E

E

S

S

L

L

Y

Y

L

L

A

A

D

D

E

E

V

V

F

F

M

M

S

S

G

G

T
|
T

A
|
A

A

A

E

E

I

I

T

T

P

P

V

V

R

R

S

S

V

V

D

D

G

G

I

I

Q

Q

V

V

G

G

E

E

G

G

R

R

C

C

G

G

P

P

V

V

T

T

K

K

R

R

I

I

Q

Q

Q

Q

A

A

F

F

F

F

G

G

L

L

F

F

T

T

G

G

E

E

T

T

E

E

D

D

K

K

W

W

G

G

W

W

L

L

D

D

Q

Q

V

V

N

N

active site: K156 (= K160)
binding 2-methylleucine: Y92 (= Y96), K156 (= K160), T254 (= T258), A255 (= A259)
binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K160), Y161 (= Y165), E190 (= E194), G193 (= G197), G216 (= G220), I217 (= I221), T218 (= T222), T254 (= T258)

2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
48% identity, 98% coverage: 5:308/309 of query aligns to 3:305/305 of 2ej0B

query
sites
2ej0B

K

K

A

A

D

G

Y

L

I

I

W

W

F

M

N

N

G

G

E

A

M

F

V

V

R

P

W

Q

E

E

D

E

A

A

K

K

V

T

H

S

V

V

M

L

S

S

H

H

A

A

L

L

H

H

Y

Y

G

G

T

T

S

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

C

A

Y

Y

D

E

S

T

H

A

K

K

G

G

P

P

V

A

V

I

F

F

R

R

H

L

R

K

E

E

H

H

M

V

Q

K

R
|
R

L

F

H

Y

D

N

S

S

A

A

K

K

I

V

Y

L

R

R

F

M

P

E

V

I

S

P

Q

F

S

A

I

P

D

E

E

E

L

L

M

E

E

E

A

A

C

I

R

K

D

E

V

V

I

V

R

R

K

R

N

N

N

G

L

Y

T

R

S

S

A

C

Y

Y

I

I

R

R

P

P

L

L

I

A

F

W

V

M

G

G

D

A

V

K

G

A

M

L

G

G

V

V

N

N

P

P

P

L

A

P

G

N

Y

N

S

P

T

A

D

E

V

V

I

M

I

V

A

A

A

A

F

W

P

E

W

W

G

G

A

A

Y

Y

L

L

G

G

A

E

E

E

A

A

L

V

E

R

Q

K

G

G

I

A

D

R

A

L

M

I

V

T

S

S

S

S

W

W

N

A

R

R

A

F

A

P

P

A

N

N

T

V

I

M

P

P

T

G

A

K

A

A

K
|
K

A

V

G

G

G

G

N

N

Y
|
Y

L

V

S

N

S

S

L

A

L

L

V

A

G

K

S

M

E

E

A

A

R

V

R

A

H

A

G

G

Y

A

Q

D

E

E

G

A

I

L

A

L

L

L

D

D

V

E

N

E

G

G

Y

Y

I

V

S

A

E
|
E

G

G

A

S

G
|
G

E
|
E

N

N

L

L

F

F

E

F

V

V

K

R

D

D

G

G

V

V

L

I

F

Y

T

A

P

L

P

E

F

H

T

S

S

V

S

N

A

-

L
|
L

P

E

G
|
G

I
|
I

T
|
T

R

R

D

D

A

S

I

V

I

I

K

R

L

I

A

A

K

K

E

D

L

L

G

G

I

Y

E

E

V

V

R

Q

E

V

Q

V

V

R

L

A

S

T

R

R

E

D

S

Q

L

L

Y

Y

L

M

A

A

D

D

E

E

V

V

F

F

M

M

S

T

G
|
G

T
|
T

A

A

A

A

E

E

I

V

T

T

P

P

V

V

R

S

S

M

V

I

D

D

G

W

I

R

Q

P

V

I

G

G

E

K

G

G

R

T

C

A

G

G

P

P

V

V

T

A

K

L

R

R

I

L

Q

R

Q

E

A

V

F

Y

F

L

G

E

L

A

F

V

T

T

G

G

E

R

T

R

E

P

D

E

K

Y

W

E

G

G

W

W

L

L

D

T

Q

Y

V

V

N

N

active site: F35 (= F37), G37 (= G39), K158 (= K160), E192 (= E194), L215 (= L218)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R60), Y163 (= Y165), E192 (= E194), G195 (= G197), E196 (= E198), L215 (= L218), G217 (= G220), I218 (= I221), T219 (= T222), G254 (= G257), T255 (= T258)

4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
47% identity, 97% coverage: 9:307/309 of query aligns to 9:306/306 of 4whxA

query
sites
4whxA

I

I

W

W

F

M

N

D

G

G

E

K

M

L

V

I

R

E

W

W

E

R

D

D

A

A

K

K

V

I

H

H

V

V

M

L

S

T

H

H

A

T

L

L

H

H

Y

Y

G

G

T

M

S

G

V

V

F
|
F

E

E

G

G

I

V

R

R

C

A

Y

Y

D

K

S

T

H

A

K

D

G

G

-

G

P

T

V

A

V

I

F

F

R

R

H

L

R

K

E

E

H

H

M

T

Q

K

R

R

L

L

H

L

D

N

S

S

A

A

K

K

I

I

Y

F

R

Q

F

M

P

D

V

V

S

P

Q

F

S

D

I

Q

D

E

E

T

L

L

M

E

E

A

A

A

C

Q

R

R

D

D

V

V

I

V

R

R

K

E

N

N

N

K

L

L

T

E

S

S

A

C

Y
|
Y

I

L

R

R

P

P

L

I

I

I

F

W

V

I

G

G

D

S

V

E

G

K

M

L

G

G

V

V

N

S

P

A

P

K

A

-

G

G

Y

N

S

T

T

I

D

H

V

V

I

A

I

I

A

A

A

A

F

W

P

P

W

W

G

G

A

A

Y

Y

L

L

G

G

A

E

E

E

A

G

L

L

E

A

Q

K

G

G

I

I

D

R

A

V

M

K

V

T

S

S

S

S

W

F

N

T

R

R

A

H

A

H

P

V

N

N

T

V

I

S

P

M

T

V

A

R

A

A

K
|
K

A

A

G

S

G

G

N

W

Y

Y

L

V

S

N

S

S

L

I

L

L

V

A

G

N

S

Q

E

E

A

A

R

T

R

A

H

D

G

G

Y

Y

Q

D

E

E

G

A

I

L

A

L

L

L

D

D

V

V

N

D

G

G

Y

Y

I

V

S

S

E
|
E

G

G

A

S

G

G

E

E

N

N

L

F

F

F

E

L

V

V

K

N

D

R

G

G

V

K

L

L

F

Y

T

T

P

P

P

D

F

L

T

-

S

A

S

S

A

C

L
|
L

P

D

G

G

I

I

T

T

R

R

D

D

A

T

I

V

I

I

K

T

L

L

A

A

K

K

E

E

L

A

G

G

I

I

E

E

V

V

R

I

E

E

Q

K

V

R

L

I

S

T

R

R

E

D

S

E

L

V

Y

Y

L

T

A

A

D

D

E

E

V

A

F

F

M

F

S

T

G

G

T

T

A
|
A

A

A

E

E

I

V

T

T

P

P

V

I

R

R

S

E

V

L

D

D

G

N

I

R

Q

T

V

I

G

G

E

G

G

G

R

A

C

R

G

G

P

P

V

I

T

T

K

E

R

K

I

L

Q

Q

Q

S

A

A

F

F

F

F

G

D

L

V

F

V

T

N

G

G

E

K

T

S

E

A

D

K

K

H

W

A

G

D

W

W

L

L

D

T

Q

K

V

I

active site: F37 (= F37), K160 (= K160), E194 (= E194), L217 (= L218)
binding alanine: Y97 (= Y96), K160 (= K160), A258 (= A259)

2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
46% identity, 98% coverage: 5:308/309 of query aligns to 3:294/294 of 2ej2A

query
sites
2ej2A

K

K

A

A

D

G

Y

L

I

I

W

W

F

M

N

N

G

G

E

A

M

F

V

V

R

P

W

Q

E

E

D

E

A

A

K

K

V

T

H

S

V

V

M

L

S

S

H

H

A

A

L

L

H

H

Y

Y

G

G

T

T

S

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

C

A

Y

Y

D

E

S

T

H

A

K

K

G

G

P

P

V

A

V

I

F

F

R

R

H

L

R

K

E

E

H

H

M

V

Q

K

R
|
R

L

F

H

Y

D

N

S

S

A

A

K

K

I

V

Y

L

R

R

F

M

P

E

V

I

S

P

Q

F

S

A

I

P

D

E

E

E

L

L

M

E

E

E

A

A

C

I

R

K

D

E

V

V

I

V

R

R

K

R

N

N

N

G

L

Y

T

R

S

S

A

C

Y
|
Y

I

I

R

R

P

P

L

L

I

A

F

W

V

M

G

G

D

A

V

K

G

A

M

L

G

G

V

V

N

N

P

P

P

L

A

P

G

N

Y

N

S

P

T

A

D

E

V

V

I

M

I

V

A

A

A

A

F

W

P

E

W

W

G

G

A

A

Y

R

L

L

G

-

A

-

E

-

A

-

L

-

E

-

Q

-

G

-

I

-

D

-

A

-

M

I

V

T

S

S

S

S

W

W

N

A

R

R

A

F

A

P

P

A

N

N

T

V

I

M

P

P

T

G

A

K

A

A

K
|
K

A

V

G

G

G

G

N

N

Y
|
Y

L

V

S

N

S

S

L

A

L

L

V

A

G

K

S

M

E

E

A

A

R

V

R

A

H

A

G

G

Y

A

Q

D

E

E

G

A

I

L

A

L

L

L

D

D

V

E

N

E

G

G

Y

Y

I

V

S

A

E
|
E

G

G

A

S

G
|
G

E
|
E

N

N

L

L

F

F

E

F

V

V

K

R

D

D

G

G

V

V

L

I

F

Y

T

A

P

L

P

E

F

H

T

S

S

V

S

N

A

-

L
|
L

P

E

G
|
G

I
|
I

T
|
T

R

R

D

D

A

S

I

V

I

I

K

R

L

I

A

A

K

K

E

D

L

L

G

G

I

Y

E

E

V

V

R

Q

E

V

Q

V

V

R

L

A

S

T

R

R

E

D

S

Q

L

L

Y

Y

L

M

A

A

D

D

E

E

V

V

F

F

M

M

S

T

G

G

T
|
T

A
|
A

A

A

E

E

I

V

T

T

P

P

V

V

R

S

S

M

V

I

D

D

G

W

I

R

Q

P

V

I

G

G

E

K

G

G

R

T

C

A

G

G

P

P

V

V

T

A

K

L

R

R

I

L

Q

R

Q

E

A

V

F

Y

F

L

G

E

L

A

F

V

T

T

G

G

E

R

T

R

E

P

D

E

K

Y

W

E

G

G

W

W

L

L

D

T

Q

Y

V

V

N

N

active site: F35 (= F37), G37 (= G39), K147 (= K160), E181 (= E194), L204 (= L218)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R60), Y94 (= Y96), Y152 (= Y165), E181 (= E194), G184 (= G197), E185 (= E198), L204 (= L218), G206 (= G220), I207 (= I221), T208 (= T222), T244 (= T258), A245 (= A259)

2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
46% identity, 98% coverage: 5:308/309 of query aligns to 3:297/297 of 2ej3A

query
sites
2ej3A

K

K

A

A

D

G

Y

L

I

I

W

W

F

M

N

N

G

G

E

A

M

F

V

V

R

P

W

Q

E

E

D

E

A

A

K

K

V

T

H

S

V

V

M

L

S

S

H

H

A

A

L

L

H

H

Y

Y

G

G

T

T

S

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

C

A

Y

Y

D

E

S

T

H

A

K

K

G

G

P

P

V

A

V

I

F

F

R

R

H

L

R

K

E

E

H

H

M

V

Q

K

R
|
R

L

F

H

Y

D

N

S

S

A

A

K

K

I

V

Y

L

R

R

F

M

P

E

V

I

S

P

Q

F

S

A

I

P

D

E

E

E

L

L

M

E

E

E

A

A

C

I

R

K

D

E

V

V

I

V

R

R

K

R

N

N

N

G

L

Y

T

R

S

S

A

C

Y

Y

I

I

R

R

P

P

L

L

I

A

F

W

V

M

G

G

D

A

V

K

G

A

M

L

G

G

V

V

N

N

P

P

P

L

A

P

G

N

Y

N

S

P

T

A

D

E

V

V

I

M

I

V

A

A

A

A

F

W

P

E

W

W

G

-

A

-

Y

-

L

-

G

-

A

-

E

-

A

-

L

V

E

R

Q

K

G

G

I

A

D

R

A

L

M

I

V

T

S

S

S

S

W

W

N

A

R

R

A

F

A

P

P

A

N

N

T

V

I

M

P

P

T

G

A

K

A

A

K
|
K

A

V

G

G

G

G

N

N

Y
|
Y

L

V

S

N

S

S

L

A

L

L

V

A

G

K

S

M

E

E

A

A

R

V

R

A

H

A

G

G

Y

A

Q

D

E

E

G

A

I

L

A

L

L

L

D

D

V

E

N

E

G

G

Y

Y

I

V

S

A

E
|
E

G

G

A

S

G
|
G

E

E

N

N

L

L

F

F

E

F

V

V

K

R

D

D

G

G

V

V

L

I

F

Y

T

A

P

L

P

E

F

H

T

S

S

V

S

N

A

-

L
|
L

P

E

G
|
G

I
|
I

T
|
T

R

R

D

D

A

S

I

V

I

I

K

R

L

I

A

A

K

K

E

D

L

L

G

G

I

Y

E

E

V

V

R

Q

E

V

Q

V

V

R

L

A

S

T

R

R

E

D

S

Q

L

L

Y

Y

L

M

A

A

D

D

E

E

V

V

F

F

M

M

S

T

G
|
G

T
|
T

A
|
A

A

A

E

E

I

V

T

T

P

P

V

V

R

S

S

M

V

I

D

D

G

W

I

R

Q

P

V

I

G

G

E

K

G

G

R

T

C

A

G

G

P

P

V

V

T

A

K

L

R

R

I

L

Q

R

Q

E

A

V

F

Y

F

L

G

E

L

A

F

V

T

T

G

G

E

R

T

R

E

P

D

E

K

Y

W

E

G

G

W

W

L

L

D

T

Q

Y

V

V

N

N

active site: F35 (= F37), G37 (= G39), K150 (= K160), E184 (= E194), L207 (= L218)
binding [1-(aminomethyl)cyclohexyl]acetic acid: G187 (= G197), G246 (= G257), T247 (= T258), A248 (= A259)
binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K160), Y155 (= Y165), E184 (= E194), G187 (= G197), L207 (= L218), G209 (= G220), I210 (= I221), T211 (= T222), G246 (= G257), T247 (= T258)

2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
46% identity, 98% coverage: 5:308/309 of query aligns to 3:297/297 of 2eiyA

query
sites
2eiyA

K

K

A

A

D

G

Y

L

I

I

W

W

F

M

N

N

G

G

E

A

M

F

V

V

R

P

W

Q

E

E

D

E

A

A

K

K

V

T

H

S

V

V

M

L

S

S

H

H

A

A

L

L

H

H

Y

Y

G

G

T

T

S

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

C

A

Y

Y

D

E

S

T

H

A

K

K

G

G

P

P

V

A

V

I

F

F

R

R

H

L

R

K

E

E

H

H

M

V

Q

K

R
|
R

L

F

H

Y

D

N

S

S

A

A

K

K

I

V

Y

L

R

R

F

M

P

E

V

I

S

P

Q

F

S

A

I

P

D

E

E

E

L

L

M

E

E

E

A

A

C

I

R

K

D

E

V

V

I

V

R

R

K

R

N

N

N

G

L

Y

T

R

S

S

A

C

Y
|
Y

I

I

R

R

P

P

L

L

I

A

F

W

V

M

G

G

D

A

V

K

G

A

M

L

G

G

V

V

N

N

P

P

P

L

A

P

G

N

Y

N

S

P

T

A

D

E

V

V

I

M

I

V

A

A

A

A

F

W

P

E

W

W

G

-

A

-

Y

-

L

-

G

-

A

-

E

-

A

-

L

V

E

R

Q

K

G

G

I

A

D

R

A

L

M

I

V

T

S

S

S

S

W

W

N

A

R

R

A

F

A

P

P

A

N

N

T

V

I

M

P

P

T

G

A

K

A

A

K
|
K

A

V

G

G

G

G

N

N

Y
|
Y

L

V

S

N

S

S

L

A

L

L

V

A

G

K

S

M

E

E

A

A

R

V

R

A

H

A

G

G

Y

A

Q

D

E

E

G

A

I

L

A

L

L

L

D

D

V

E

N

E

G

G

Y

Y

I

V

S

A

E
|
E

G

G

A

S

G
|
G

E
|
E

N

N

L

L

F

F

E

F

V

V

K

R

D

D

G

G

V

V

L

I

F

Y

T

A

P

L

P

E

F

H

T

S

S

V

S

N

A

-

L
|
L

P

E

G
|
G

I
|
I

T
|
T

R

R

D

D

A

S

I

V

I

I

K

R

L

I

A

A

K

K

E

D

L

L

G

G

I

Y

E

E

V

V

R

Q

E

V

Q

V

V

R

L

A

S

T

R

R

E

D

S

Q

L

L

Y

Y

L

M

A

A

D

D

E

E

V

V

F

F

M

M

S

T

G
|
G

T
|
T

A
|
A

A

A

E

E

I

V

T

T

P

P

V

V

R

S

S

M

V

I

D

D

G

W

I

R

Q

P

V

I

G

G

E

K

G

G

R

T

C

A

G

G

P

P

V

V

T

A

K

L

R

R

I

L

Q

R

Q

E

A

V

F

Y

F

L

G

E

L

A

F

V

T

T

G

G

E

R

T

R

E

P

D

E

K

Y

W

E

G

G

W

W

L

L

D

T

Q

Y

V

V

N

N

active site: F35 (= F37), G37 (= G39), K150 (= K160), E184 (= E194), L207 (= L218)
binding 4-methyl valeric acid: F35 (= F37), Y94 (= Y96), T247 (= T258), A248 (= A259)
binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K160), Y155 (= Y165), E184 (= E194), G187 (= G197), E188 (= E198), L207 (= L218), G209 (= G220), I210 (= I221), T211 (= T222), G246 (= G257), T247 (= T258)

1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
46% identity, 98% coverage: 5:308/309 of query aligns to 3:297/297 of 1wrvA

query
sites
1wrvA

K

K

A

A

D

G

Y

L

I

I

W

W

F

M

N

N

G

G

E

A

M

F

V

V

R

P

W

Q

E

E

D

E

A

A

K

K

V

T

H

S

V

V

M

L

S

S

H

H

A

A

L

L

H

H

Y

Y

G

G

T

T

S

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

C

A

Y

Y

D

E

S

T

H

A

K

K

G

G

P

P

V

A

V

I

F

F

R

R

H

L

R

K

E

E

H

H

M

V

Q

K

R
|
R

L

F

H

Y

D

N

S

S

A

A

K

K

I

V

Y

L

R

R

F

M

P

E

V

I

S

P

Q

F

S

A

I

P

D

E

E

E

L

L

M

E

E

E

A

A

C

I

R

K

D

E

V

V

I

V

R

R

K

R

N

N

N

G

L

Y

T

R

S

S

A

C

Y

Y

I

I

R

R

P

P

L

L

I

A

F

W

V

M

G

G

D

A

V

K

G

A

M

L

G

G

V

V

N

N

P

P

P

L

A

P

G

N

Y

N

S

P

T

A

D

E

V

V

I

M

I

V

A

A

A

A

F

W

P

E

W

W

G

-

A

-

Y

-

L

-

G

-

A

-

E

-

A

-

L

V

E

R

Q

K

G

G

I

A

D

R

A

L

M

I

V

T

S

S

S

S

W

W

N

A

R

R

A

F

A

P

P

A

N

N

T

V

I

M

P

P

T

G

A

K

A

A

K
|
K

A

V

G

G

G

G

N

N

Y
|
Y

L

V

S

N

S

S

L

A

L

L

V

A

G

K

S

M

E

E

A

A

R

V

R

A

H

A

G

G

Y

A

Q

D

E

E

G

A

I

L

A

L

L

L

D

D

V

E

N

E

G

G

Y

Y

I

V

S

A

E
|
E

G

G

A

S

G
|
G

E

E

N

N

L

L

F

F

E

F

V

V

K

R

D

D

G

G

V

V

L

I

F

Y

T

A

P

L

P

E

F

H

T

S

S

V

S

N

A

-

L
|
L

P

E

G
|
G

I
|
I

T
|
T

R

R

D

D

A

S

I

V

I

I

K

R

L

I

A

A

K

K

E

D

L

L

G

G

I

Y

E

E

V

V

R

Q

E

V

Q

V

V

R

L

A

S

T

R

R

E

D

S

Q

L

L

Y

Y

L

M

A

A

D

D

E

E

V

V

F

F

M

M

S

T

G

G

T
|
T

A

A

A

A

E

E

I

V

T

T

P

P

V

V

R

S

S

M

V

I

D

D

G

W

I

R

Q

P

V

I

G

G

E

K

G

G

R

T

C

A

G

G

P

P

V

V

T

A

K

L

R

R

I

L

Q

R

Q

E

A

V

F

Y

F

L

G

E

L

A

F

V

T

T

G

G

E

R

T

R

E

P

D

E

K

Y

W

E

G

G

W

W

L

L

D

T

Q

Y

V

V

N

N

active site: F35 (= F37), G37 (= G39), K150 (= K160), E184 (= E194), L207 (= L218)
binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K160), Y155 (= Y165), E184 (= E194), G187 (= G197), L207 (= L218), G209 (= G220), I210 (= I221), T211 (= T222), T247 (= T258)

6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
42% identity, 97% coverage: 9:307/309 of query aligns to 9:300/301 of 6thqB

query
sites
6thqB

I

V

W

W

F

L

N

D

G

G

E

R

M

L

V

V

R

D

W

E

E

E

D

E

A

A

K

K

V

V

H

T

V

V

M

L

S

S

H

P

A

S

L

L

H

N

Y

Y

G

G

T

F

S

G

V

V

F
|
F

E

E

G

G

I

I

R

R

C

A

Y

Y

D

W

S

N

H

G

K

E

G

N

P

L

V

Y

V

V

F

F

R

R

H

L

R

R

E

D

H

H

M

M

Q

E

R
|
R

L

L

H

L

D

R

S

S

A

A

K

K

I

I

Y

I

R

G

F

L

P

D

V

V

S

P

Q

Y

S

T

I

A

D

E

E

E

L

L

M

S

E

K

A

A

C

V

R

V

D

E

V

T

I

V

R

R

K

A

N

N

N

G

L

F

T

K

S

E

-

D

A

L

Y
|
Y

I

I

R

R

P

P

L

V

I

A

F

Y

V

I

G

S

D

K

V

P

G

Q

M

I

G

S

V

L

N

D

P

V

P

-

A

R

G

G

Y

L

S

Q

T

A

D

S

V

V

I

A

I

I

A

A

A

A

F

I

P

P

W

F

G

G

A

K

Y

Y

L

L

G

K

A

V

E

E

A

-

L

-

E

-

Q

-

G

G

I

V

D

R

A

A

M

A

V

V

S

V

S

S

W

W

N

R

R

R

A

V

A

H

P

T

N

S

T

M

I

M

P

P

T

V

A

M

A

A

K
|
K

A

A

G

T

G

G

N

I

Y
|
Y

L

L

S

N

S

S

L

I

L

M

V

A

G

A

S

V

E

E

A

A

R

R

R

A

H

R

G

G

Y

Y

Q

D

E

E

G

A

I

I

A

M

L

L

D

N

V

A

N

E

G

G

Y

K

I

V

S

V

E
|
E

G

G

A

S

G
|
G

E
|
E

N
|
N

L

I

F

F

E

I

V

V

K

R

D

R

G

G

V

V

L

L

F

M

T

T

P

P

P

P

F

L

T

E

S

D

S

G

A

I

L
|
L

P

E

G
|
G

I
|
I

T
|
T

R

R

D

E

A

T

I

V

I

I

K

S

L

I

A

A

K

G

E

D

L

L

G

G

I

I

E

P

V

L

R

L

E

E

Q

K

V

S

L

I

S

T

R

R

E

E

S

E

L

L

Y

Y

L

A

A

A

D

D

E

E

V

A

F

F

M

F

S

V

G
|
G

T
|
T

A
|
A

A

A

E

E

I

I

T

T

P

P

V

I

R

I

S

E

V

I

D

D

G

G

I

R

Q

V

V

L

G

-

E

-

G

-

R

Q

C

R

G

G

P

P

V

I

T

T

K

Q

R

K

I

I

Q

A

Q

E

A

T

F

Y

F

R

G

R

L

I

F

V

T

L

G

G

E

K

T

E

E

E

D

K

K

Y

W

L

G

P

W

W

L

L

D

T

Q

P

V

V

active site: F37 (= F37), K156 (= K160), E190 (= E194), L214 (= L218)
binding pyridoxal-5'-phosphate: R60 (= R60), K156 (= K160), Y161 (= Y165), E190 (= E194), N195 (= N199), L214 (= L218), G216 (= G220), I217 (= I221), T218 (= T222), T254 (= T258)
binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R60), Y97 (= Y96), K156 (= K160), Y161 (= Y165), E190 (= E194), G193 (= G197), E194 (= E198), N195 (= N199), G216 (= G220), I217 (= I221), T218 (= T222), G253 (= G257), T254 (= T258), A255 (= A259)

5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
40% identity, 94% coverage: 8:296/309 of query aligns to 3:284/290 of 5mr0D

query
sites
5mr0D

Y

Y

I

V

W

Y

F

M

N

D

G

G

E

E

M

F

V

V

R

P

W

E

E

N

D

E

A

A

K

K

V

V

H

S

V

I

M

F

S

D

H

H

A

G

L

F

H

L

Y

Y

G

G

T

D

S

G

V

V

F
|
F

E

E

G
|
G

I

I

R

R

C

A

Y

Y

D

N

S

G

H

R

K

-

G

-

P

-

V

-

V

V

F

F

R

R

H

L

R

K

E

E

H

H

M

I

Q

D

R
|
R

L

L

H

Y

D

D

S

S

A

A

K

K

I

A

Y

I

R

D

F

L

P

E

V

I

S

P

Q

I

S

T

I

K

D

E

E

E

L

F

M

M

E

E

A

I

C

I

R

L

D

E

V

T

I

L

R

R

K

K

N

N

N

N

L

L

T

R

S

D

A

A

Y

Y

I

I

R

R

P

P

L

I

I

V

F

T

V

R

G

G

D

I

V

G

G

D

M

L

G
|
G

V
x
L

N

D

P

P

P

R

A

K

G

C

Y

Q

S

N

T

P

D

S

V

I

I

I

I

V

A

I

A

T

F

K

P

P

W

W

G

G

A

K

Y

L

L

Y

G

G

A

-

E

D

A

L

L

Y

E

E

Q

K

G

G

I

L

D

T

A

A

M

I

V

T

S

V

S

A

W

V

N

R

R

R

A

N

A

S

P

F

N

D

T

A

I

L

P

P

T

P

A

N

A

I

K
|
K

A

S

G

L

G

-

N

N

Y
|
Y

L

L

S

N

S

N

L

I

L

L

V

A

G

K

S

I

E

E

A

A

R

N

R

A

H

K

G

G

Y

G

Q

D

E

E

G

A

I

I

A

F

L

L

D

D

V

R

N

N

G

G

Y

Y

I

V

S

S

E
|
E

G

G

A

S

G
|
G

E
x
D

N

N

L

I

F

F

E

V

V

V

K

K

D

N

G

G

V

A

L

I

F

T

T

T

P

P

P

P

F

-

T

T

S

I

S

N

A

N

L
|
L

P

R

G

G

I
|
I

T
|
T

R

R

D

E

A

A

I

V

I

I

K

E

L

I

A

I

K

N

E

R

L

L

G

G

I

I

E

P

V

F

R

K

E

E

Q

T

V

N

L

I

S

G

R

L

E

Y

S

D

L

L

Y

Y

L

T

A

A

D

D

E

E

V

V

F

F

M

V

S

T

G
|
G

T
|
T

A

A

A

A

E

E

I

I

T

A

P

P

V

I

R

V

S

V

V

I

D

D

G

G

I

R

Q

K

V

I

G

G

E

D

G

G

R

K

C

P

G

G

P

E

V

I

T

T

K

R

R

K

I

L

Q

M

Q

E

A

E

F

F

F

S

G

K

L

L

F

T

T

E

G

S

E

E

active site: F32 (= F37), G34 (= G39), K150 (= K160), E183 (= E194), L206 (= L218)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R60), G100 (= G109), L101 (≠ V110), K150 (= K160), Y154 (= Y165), E183 (= E194), G186 (= G197), D187 (≠ E198), L206 (= L218), I209 (= I221), T210 (= T222), G245 (= G257), T246 (= T258)

5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
43% identity, 91% coverage: 9:289/309 of query aligns to 5:278/290 of 5e25A

query
sites
5e25A

I

V

W

Y

F

M

N

N

G

G

E

E

M

F

V

V

R

P

W

E

E

S

D

Q

A

A

K

K

V

V

H

S

V

V

M

F

S

D

H

H

A

G

L

F

H

L

Y

Y

G

G

T

D

S

G

V

V

F
|
F

E

E

G
|
G

I

I

R

R

C

A

Y

Y

D

N

S

G

H

K

K

-

G

-

P

-

V

-

V

V

F

F

R

K

H

L

R

Y

E

E

H

H

M

I

Q

D

R
|
R

L

L

H

Y

D

D

S

C

A

A

K

R

I

V

Y

I

R

D

F

L

P

K

V

I

S

P

Q

L

S

S

I

K

D

E

E

E

L

F

M

A

E

E

A

A

C

I

R

L

D

E

V

T

I

L

R

R

K

R

N

N

N

N

L

L

T

R

S

D

A

A

Y
|
Y

I

I

R

R

P

P

L

I

I

V

F

T

V

R

G

G

D

A

V

G

G

D

M

L

G

G

V

L

N

D

P

P

P

R

A

K

G

C

Y

P

S

S

T

P

D

N

V

V

I

I

I

I

A

I

A

T

F

K

P

P

W

W

G

G

A

K

Y

L

L

Y

G

G

A

-

E

D

A

L

L

Y

E

E

Q

K

G

G

I

L

D

K

A

A

M

I

V

T

S

V

S

A

W

I

N

R

R

R

A

N

A

A

P

I

N

D

T

S

I

L

P

P

T

P

A

N

A

I

K
|
K

A

S

G

L

G

-

N

N

Y
|
Y

L

L

S

N

S

N

L

I

L

L

V

A

G

K

S

I

E

E

A

A

R

N

R

A

H

K

G

G

Y

G

Q

D

E

E

G

A

I

I

A

F

L

L

D

D

V

H

N

N

G

G

Y

Y

I

I

S

S

E
|
E

G

G

A

S

G
|
G

E
x
D

N

N

L

I

F

F

E

I

V

V

K

K

D

N

G

G

V

T

L

I

F

T

T

T

P

P

P

P

F

-

T

T

S

L

S

N

A

N

L
|
L

P

K

G
|
G

I
|
I

T
|
T

R

R

D

Q

A

V

I

V

I

I

K

E

L

L

A

I

K

N

E

E

L

L

G

E

I

I

E

P

V

F

R

R

E

E

Q

A

V

N

L

I

S

G

R

L

E

F

S

D

L

L

Y

Y

L

S

A

A

D

D

E

E

V

I

F

F

M

V

S

T

G
|
G

T
|
T

A
|
A

A

A

E

E

I

I

T

A

P

P

V

V

R

T

S

Y

V

I

D

D

G

G

I

R

Q

T

V

V

G

G

E

N

G

G

R

K

C

P

G

G

P

K

V

V

T

T

K

K

R

M

I

L

Q

M

Q

E

A

K

F

F

active site: F33 (= F37), G35 (= G39), K151 (= K160), E184 (= E194), L207 (= L218)
binding 2-oxoglutaric acid: Y88 (= Y96), K151 (= K160), T247 (= T258), A248 (= A259)
binding pyridoxal-5'-phosphate: R52 (= R60), K151 (= K160), Y155 (= Y165), E184 (= E194), G187 (= G197), D188 (≠ E198), L207 (= L218), G209 (= G220), I210 (= I221), T211 (= T222), G246 (= G257), T247 (= T258)

6h65C Crystal structure of the branched-chain-amino-acid aminotransferase from haliangium ochraceum
36% identity, 96% coverage: 8:304/309 of query aligns to 6:303/308 of 6h65C

query
sites
6h65C

Y

Y

I

G

W

W

F

M

N

N

G

G

E

Q

M

C

V

I

R

P

W

W

E

D

D

Q

A

C

K

S

V

L

H

H

V

V

M

S

S

T

H

Q

A

A

L

A

H

F

Y

F

G

G

T

A

S

S

V

L

F
|
F

E

E

G

G

I

V

R

R

C

A

Y

Y

-

W

-

N

D

A

S

E

H

R

K

E

G

Q

P

L

V

Y

V

V

F

F

R

R

H

L

R

D

E

E

H

H

M

L

Q

R

R
|
R

L

L

H

E

D

Q

S

S

A

A

K

K

I

M

Y

L

R

R

F

M

P

K

V

L

S

S

Q

M

S

P

I

I

D

A

E

D

L

I

M

R

E

Q

A

G

C

V

R

L

D

E

V

L

I

L

R

R

K

A

N

N

N

E

L

F

T

R

S

S

A

D

Y

V

I

-

R

-

P

H

L

L

I

Y

F

V

V

A

G

S

D

Y

V

F

G

G

M

I

G

N

V

H

N

D

P

P

P

D

A

P

G

L

Y

F

S

P

T

T

D

D

V

D

I

T

I

-

A

-

A

-

F

-

P

-

W

-

G

G

A

V

Y

Y

L

V

G

T

A

G

E

T

A

A

L

V

E

S

Q

R

-

L

-

P

-

L

-

V

-

H

-

T

G

G

I

I

D

S

A

A

M

C

V

M

S

S

S

S

W

W

N

R

R

R

A

I

A

S

P

D

N

D

T

S

I

V

P

P

T

P

A

R

A

I

K
|
K

A

I

G

G

G

A

N

N

Y
|
Y

L

Q

S

N

S

S

L

R

L

L

V

A

G

Q

S

T

E

E

A

A

R

R

R

V

H

N

G

G

Y

Y

Q

H

E

T

G

S

I

V

A

L

L

L

D

N

V

S

N

R

G

G

Y

K

I

V

S

S

E
|
E

G

T

A

P

G

G

E
x
A

N

C

L

L

F

L

E

M

V

V

K

R

D

D

G

G

V

R

L

V

F

I

T

S

P

P

P

P

F

V

T

T

S

A

S

D

A

I

L
|
L

P

E

G
x
S

I
x
V

T
|
T

R

R

D

K

A

T

I

L

I

M

K

S

L

L

A

S

K

E

-

A

E

E

L

L

G

D

I

S

E

P

V

V

R

I

E

E

Q

R

V

D

L

M

S

D

R

R

E

T

S

E

L

L

Y

Y

L

I

A

A

D

E

E

E

V

V

F

F

M

L

S

C

G
|
G

T
|
T

A

I

A

A

E

E

I

I

T

L

P

P

V

V

R

T

S

T

V

I

D

D

G

R

I

I

Q

Q

V

V

G

G

E

D

G

G

R

E

C

V

G

G

P

P

V

V

T

T

K

R

R

R

I

L

Q

Q

Q

E

A

L

F

Y

F

F

G

G

L

V

F

T

T

S

G

G

E

Q

T

L

E

E

D

A

K

Y

W

K

G

S

W

W

L

L

active site: F35 (= F37), K158 (= K160), E192 (= E194), L216 (= L218)
binding pyridoxal-5'-phosphate: R60 (= R60), K158 (= K160), Y163 (= Y165), E192 (= E194), A196 (≠ E198), L216 (= L218), S218 (≠ G220), V219 (≠ I221), T220 (= T222), G256 (= G257), T257 (= T258)

6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
36% identity, 97% coverage: 8:307/309 of query aligns to 5:304/307 of 6q8eA

query
sites
6q8eA

Y

Y

I

L

W

W

F

W

N

N

G

H

E

R

M

I

V

I

R

P

W

W

E

E

D

E

A

A

K

T

V

V

H

H

V

L

M

T

S

D

H

Y

A

W

L

W

H

A

Y

S

G

V

T

T

S

A

V

V

F
|
F

E

E

G

G

I

I

R

R

C

G

Y

Y

-

W

D

N

S

N

H

A

K

E

G

G

P

E

V

M

-

Y

V

I

F

F

R

R

H

L

R

E

E

D

H

H

M

A

Q

R

R
|
R

L

L

H

E

D

Q

S

S

A

M

K