SitesBLAST
Comparing 199535 FitnessBrowser__MR1:199535 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3ht5A Crystal structure of ilve a branched chain amino acid transaminase from mycobacterium tuberculosis (see paper)
39% identity, 90% coverage: 31:358/363 of query aligns to 3:334/335 of 3ht5A
- active site: K171 (= K198)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R68 (= R95), K171 (= K198), Y176 (= Y203), E207 (= E233), G210 (≠ A236), M211 (= M237), N212 (= N238), L235 (= L258), G237 (≠ S260), I238 (= I261), T239 (= T262), T281 (= T304)
A0R066 Branched-chain-amino-acid aminotransferase; BCAT; EC 2.6.1.42 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see 2 papers)
38% identity, 98% coverage: 3:358/363 of query aligns to 10:367/368 of A0R066
- R101 (= R95) binding
- Y209 (= Y203) binding
- K299 (≠ A289) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
- T314 (= T304) binding
3dtgA Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design
38% identity, 98% coverage: 3:358/363 of query aligns to 5:362/363 of 3dtgA
- active site: K199 (= K198)
- binding O-benzylhydroxylamine: F72 (= F71), Y171 (= Y169), Y204 (= Y203)
- binding pyridoxal-5'-phosphate: R96 (= R95), R189 (= R188), K199 (= K198), Y204 (= Y203), E235 (= E233), G238 (≠ A236), L263 (= L258), I266 (= I261), T267 (= T262), T309 (= T304)
3dtfA Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design (see paper)
38% identity, 98% coverage: 3:358/363 of query aligns to 5:362/363 of 3dtfA
5u3fA Structure of mycobacterium tuberculosis ilve, a branched-chain amino acid transaminase, in complex with d-cycloserine derivative (see paper)
37% identity, 90% coverage: 31:358/363 of query aligns to 2:325/326 of 5u3fA
- active site: K162 (= K198)
- binding (5-hydroxy-6-methyl-4-{[(3-oxo-2,3-dihydro-1,2-oxazol-4-yl)amino]methyl}pyridin-3-yl)methyl dihydrogen phosphate: G45 (= G73), R67 (= R95), Y110 (= Y137), K162 (= K198), Y167 (= Y203), E198 (= E233), G201 (≠ A236), M202 (= M237), N203 (= N238), L226 (= L258), G228 (≠ S260), I229 (= I261), T230 (= T262), T272 (= T304)
3uzoA Crystal structures of branched-chain aminotransferase from deinococcus radiodurans complexes with alpha-ketoisocaproate and l-glutamate suggest its radio-resistance for catalysis (see paper)
37% identity, 93% coverage: 23:358/363 of query aligns to 5:335/335 of 3uzoA
- active site: K179 (= K198)
- binding glutamic acid: Y48 (= Y66), F53 (= F71), R122 (= R139), V134 (= V151), Y152 (= Y169), T281 (= T304), A282 (= A305)
- binding pyridoxal-5'-phosphate: R77 (= R95), K179 (= K198), Y184 (= Y203), E215 (= E233), G217 (= G235), A218 (= A236), A219 (≠ M237), N220 (= N238), L240 (= L258), S242 (= S260), I243 (= I261), T244 (= T262), G280 (= G303), T281 (= T304)
3uzbA Crystal structures of branched-chain aminotransferase from deinococcus radiodurans complexes with alpha-ketoisocaproate and l-glutamate suggest its radio-resistance for catalysis (see paper)
37% identity, 93% coverage: 23:358/363 of query aligns to 5:335/335 of 3uzbA
- active site: K179 (= K198)
- binding 2-oxo-4-methylpentanoic acid: Y120 (= Y137), K179 (= K198), T281 (= T304), A282 (= A305)
- binding pyridoxal-5'-phosphate: R77 (= R95), K179 (= K198), Y184 (= Y203), E215 (= E233), G217 (= G235), A218 (= A236), A219 (≠ M237), N220 (= N238), S242 (= S260), I243 (= I261), T244 (= T262), G280 (= G303), T281 (= T304)
6jifB Crystal structures of branched-chain aminotransferase from pseudomonas sp. Uw4 (see paper)
36% identity, 92% coverage: 24:358/363 of query aligns to 6:334/334 of 6jifB
- binding pyridoxal-5'-phosphate: R77 (= R95), R169 (= R188), K179 (= K198), Y184 (= Y203), E215 (= E233), S218 (≠ A236), A219 (≠ M237), N220 (= N238), L239 (= L258), G241 (≠ S260), I242 (= I261), T243 (= T262), G279 (= G303), T280 (= T304)
3uyyB Crystal structures of branched-chain aminotransferase from deinococcus radiodurans complexes with alpha-ketoisocaproate and l-glutamate suggest its radio-resistance for catalysis (see paper)
37% identity, 93% coverage: 23:358/363 of query aligns to 7:337/337 of 3uyyB
- active site: K181 (= K198)
- binding pyridoxal-5'-phosphate: R79 (= R95), R171 (= R188), K181 (= K198), Y186 (= Y203), E217 (= E233), G219 (= G235), A220 (= A236), A221 (≠ M237), S244 (= S260), I245 (= I261), T246 (= T262), G282 (= G303), T283 (= T304)
5i5tA X-ray crystal structure at 2.31a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a tetrahydroquinoline compound and an internal aldimine linked plp cofactor. (see paper)
34% identity, 90% coverage: 19:346/363 of query aligns to 19:351/361 of 5i5tA
- active site: K198 (= K198)
- binding (3R)-3-methyl-1,2,3,4-tetrahydroquinoline-8-sulfonamide: F26 (= F26), F71 (= F71), Y169 (= Y169), K198 (= K198), T236 (≠ A236), A310 (= A305)
- binding pyridoxal-5'-phosphate: R95 (= R95), R188 (= R188), K198 (= K198), Y203 (= Y203), E233 (= E233), T236 (≠ A236), M237 (= M237), N238 (= N238), L262 (= L258), G264 (≠ S260), V265 (≠ I261), V266 (≠ T262), G308 (= G303), T309 (= T304)
5i5vA X-ray crystal structure at 1.94a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a thienopyrimidine compound and an internal aldimine linked plp cofactor. (see paper)
34% identity, 90% coverage: 19:346/363 of query aligns to 18:350/360 of 5i5vA
- active site: K197 (= K198)
- binding 3,5-dimethyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-6-carboxylic acid: F25 (= F26), K74 (= K75), Y136 (= Y137), Y168 (= Y169), A309 (= A305)
- binding pyridoxal-5'-phosphate: R94 (= R95), R187 (= R188), K197 (= K198), Y202 (= Y203), E232 (= E233), T235 (≠ A236), M236 (= M237), L261 (= L258), G263 (≠ S260), V264 (≠ I261), V265 (≠ T262), G307 (= G303), T308 (= T304)
5i5sA X-ray crystal structure at 2.06a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a benzisoxazole compound and an internal aldimine linked plp cofactor. (see paper)
34% identity, 90% coverage: 19:346/363 of query aligns to 19:351/361 of 5i5sA
- active site: K198 (= K198)
- binding 2-(1,2-benzoxazol-3-yl)ethanoic acid: F26 (= F26), F71 (= F71), K198 (= K198), T236 (≠ A236), T309 (= T304), A310 (= A305)
- binding pyridoxal-5'-phosphate: R95 (= R95), R188 (= R188), K198 (= K198), Y203 (= Y203), E233 (= E233), T236 (≠ A236), M237 (= M237), N238 (= N238), L262 (= L258), G264 (≠ S260), V265 (≠ I261), V266 (≠ T262), G308 (= G303), T309 (= T304)
2hdkB Crystal structure of cys315ala-cys318ala mutant of human mitochondrial branched chain aminotransferase (see paper)
34% identity, 88% coverage: 26:346/363 of query aligns to 28:353/363 of 2hdkB
- active site: K200 (= K198)
- binding 2-oxo-4-methylpentanoic acid: G310 (= G303), T311 (= T304), A312 (= A305)
- binding pyridoxal-5'-phosphate: R97 (= R95), K200 (= K198), Y205 (= Y203), E235 (= E233), T238 (≠ A236), L264 (= L258), G266 (≠ S260), V267 (≠ I261), V268 (≠ T262), G310 (= G303), T311 (= T304)
5i5yA X-ray crystal structure at 1.81a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with an aryl acetate compound and an internal aldimine linked plp cofactor. (see paper)
34% identity, 88% coverage: 26:346/363 of query aligns to 28:353/363 of 5i5yA
- active site: K200 (= K198)
- binding {2-[(5-methyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-6-carbonyl)amino]phenyl}acetic acid: F28 (= F26), Y68 (= Y66), F73 (= F71), K77 (= K75), Y139 (= Y137), V153 (= V151), Y171 (= Y169), T238 (≠ A236), T311 (= T304), A312 (= A305)
- binding pyridoxal-5'-phosphate: R97 (= R95), R190 (= R188), K200 (= K198), Y205 (= Y203), E235 (= E233), T238 (≠ A236), L264 (= L258), G266 (≠ S260), V267 (≠ I261), V268 (≠ T262), T311 (= T304)
5i5uA X-ray crystal structure at 2.40a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a tetrahydronaphthalenyl compound and an internal aldimine linked plp cofactor. (see paper)
34% identity, 88% coverage: 26:346/363 of query aligns to 28:353/363 of 5i5uA
- active site: K200 (= K198)
- binding 2-hydroxy-N-[(1R)-1,2,3,4-tetrahydronaphthalen-1-yl]acetamide: F28 (= F26), Y68 (= Y66), F73 (= F71), V153 (= V151), K200 (= K198), Y205 (= Y203), T238 (≠ A236), G310 (= G303), T311 (= T304), A312 (= A305)
- binding pyridoxal-5'-phosphate: R97 (= R95), R190 (= R188), K200 (= K198), Y205 (= Y203), E235 (= E233), T238 (≠ A236), M239 (= M237), L264 (= L258), G266 (≠ S260), V267 (≠ I261), V268 (≠ T262), G310 (= G303), T311 (= T304)
O15382 Branched-chain-amino-acid aminotransferase, mitochondrial; BCAT(m); Placental protein 18; PP18; EC 2.6.1.42 from Homo sapiens (Human) (see 6 papers)
34% identity, 88% coverage: 26:346/363 of query aligns to 57:382/392 of O15382
- Y168 (= Y137) binding
- R170 (= R139) to Q: in HVLI; reduced catalytic activity; dbSNP:rs749866079
- T186 (≠ S155) to R: in dbSNP:rs11548193
- K229 (= K198) modified: N6-(pyridoxal phosphate)lysine
- E264 (= E233) to K: in HVLI; reduced catalytic activity; dbSNP:rs767653663
- C342 (≠ A306) mutation to A: Reduces activity about 6-fold.
- C345 (≠ S309) mutation to A: Slight reduction of activity.
5i60A X-ray crystal structure at 2.12a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a biarl amide compound and an internal aldimine linked plp cofactor. (see paper)
34% identity, 88% coverage: 26:346/363 of query aligns to 24:349/359 of 5i60A
- active site: K196 (= K198)
- binding (2-{[4-(1H-pyrazol-3-yl)phenyl]carbamoyl}phenyl)acetic acid: F24 (= F26), Y135 (= Y137), G165 (= G167), Y167 (= Y169), T234 (≠ A236), G306 (= G303), A308 (= A305)
- binding pyridoxal-5'-phosphate: R93 (= R95), R186 (= R188), K196 (= K198), Y201 (= Y203), E231 (= E233), T234 (≠ A236), M235 (= M237), L260 (= L258), G262 (≠ S260), V263 (≠ I261), V264 (≠ T262), T307 (= T304)
5hneA X-ray crystal structure of human mitochondrial branched chain aminotransferase (bcatm) complexed with a 2-aryl benzimidazole compound and an internal aldimine linked plp cofactor (see paper)
34% identity, 88% coverage: 26:346/363 of query aligns to 24:349/359 of 5hneA
- active site: K196 (= K198)
- binding 1-[(1R,3S)-3-{[(5-bromothiophen-2-yl)carbonyl]amino}cyclohexyl]-N-methyl-2-(pyridin-2-yl)-1H-benzimidazole-5-carboxamide: F24 (= F26), F69 (= F71), K73 (= K75), Y167 (= Y169), Q218 (= Q220), V232 (≠ A234), G233 (= G235), T234 (≠ A236), M235 (= M237), A308 (= A305), C312 (≠ S309)
- binding pyridoxal-5'-phosphate: R93 (= R95), K196 (= K198), Y201 (= Y203), E231 (= E233), T234 (≠ A236), N236 (= N238), L260 (= L258), G262 (≠ S260), V263 (≠ I261), V264 (≠ T262), G306 (= G303), T307 (= T304)
5cr5A X-ray crystal structure at 1.61a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a biphenyl pyrrolidine ether compound and an internal aldimine linked plp cofactor. (see paper)
34% identity, 88% coverage: 26:346/363 of query aligns to 23:348/358 of 5cr5A
- active site: K195 (= K198)
- binding 3-({(3R)-1-[(5-bromothiophen-2-yl)carbonyl]pyrrolidin-3-yl}oxy)-N-methyl-2'-[(methylsulfonyl)amino]biphenyl-4-carboxamide: F23 (= F26), F68 (= F71), Y166 (= Y169), V175 (≠ I178), Q217 (= Q220), V231 (≠ A234), G232 (= G235), T233 (≠ A236), M234 (= M237), G305 (= G303), A307 (= A305), C311 (≠ S309)
- binding pyridoxal-5'-phosphate: R92 (= R95), K195 (= K198), Y200 (= Y203), E230 (= E233), T233 (≠ A236), N235 (= N238), L259 (= L258), G261 (≠ S260), V262 (≠ I261), V263 (≠ T262), T306 (= T304)
2a1hA X-ray crystal structure of human mitochondrial branched chain aminotransferase (bcatm) complexed with gabapentin (see paper)
34% identity, 88% coverage: 26:346/363 of query aligns to 28:353/363 of 2a1hA
- active site: K200 (= K198)
- binding [1-(aminomethyl)cyclohexyl]acetic acid: Y68 (= Y66), R141 (= R139), V153 (= V151), T238 (≠ A236), G310 (= G303), T311 (= T304), A312 (= A305)
- binding pyridoxal-5'-phosphate: R97 (= R95), R190 (= R188), K200 (= K198), Y205 (= Y203), E235 (= E233), T238 (≠ A236), M239 (= M237), N240 (= N238), L264 (= L258), G266 (≠ S260), V267 (≠ I261), V268 (≠ T262), T311 (= T304)
Query Sequence
>199535 FitnessBrowser__MR1:199535
MEIKYDLKPASERRTEQFKPEGNVGFGSLRTDHMFLMDYHDGEWRDPRIVPYGPFEMAPG
AMALHYGQSIFEGAKAFMHEDGEIYTFRINKNAERMNRSADIVCIPNIDEQMQIDAINAL
IDVDRLWFPMQEGACLYIRPFIFATEDRLSVSPSSRYTFCVVLSPSGAYYAAGVNKGIRL
LISKTYHRAVSGGTGASKAAGNYAASLRAGKAAAQFGASQVLYLDANNQQIEEAGAMNHF
HILKDGTVIIPTFTDTILKSITSQSIMELSELLGCEVRQETVMLDKFIADIESGEIIEAG
GFGTAAVVSAVGSYIFEDGRVVTVGNGEVGEHIQRIYKLYTDIQKGHVQGPEGWVKRVER
VAP
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory