SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 199618 FitnessBrowser__MR1:199618 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0A9P0 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein; EC 1.8.1.4 from Escherichia coli (strain K12) (see 2 papers)
86% identity, 100% coverage: 1:475/475 of query aligns to 1:474/474 of P0A9P0

query
sites
P0A9P0

M
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M

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L

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M1 (= M1) modified: Initiator methionine, Removed
K220 (≠ R221) modified: N6-acetyllysine

4jdrA Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase from escherichia coli (see paper)
86% identity, 99% coverage: 2:473/475 of query aligns to 1:471/471 of 4jdrA

query
sites
4jdrA

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active site: P15 (= P16), L40 (= L41), C44 (= C45), C49 (= C50), S52 (= S53), E77 (= E78), P78 (= P79), I184 (= I186), E188 (= E190), V324 (= V326), H442 (= H444), H444 (= H446), E449 (= E451), N467 (= N469), P468 (= P470)
binding flavin-adenine dinucleotide: G12 (= G13), G14 (= G15), P15 (= P16), A16 (= A17), E35 (= E36), R36 (= R37), Y37 (≠ F38), V43 (= V44), C44 (= C45), G48 (= G49), C49 (= C50), K53 (= K54), L115 (≠ F116), G116 (= G117), A144 (= A146), G145 (= G147), I185 (= I187), G311 (= G313), D312 (= D314), M318 (= M320), L319 (= L321), A320 (= A322), H321 (= H323)

1bhyA Low temperature middle resolution structure of p64k from masc data (see paper)
64% identity, 95% coverage: 19:471/475 of query aligns to 19:481/482 of 1bhyA

query
sites
1bhyA

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G

I

V

A

A

Y

Y

T

T

D

S

P

P

E

E

V

V

A

A

W

W

V

V

G

G

L

E

T

T

E

E

K

L

E

S

A

A

K

K

E

A

Q

S

G

A

I

R

A

K

Y

I

E

T

T

K

A

A

T

N

F

F

P

P

W

W

A

A

S

S

G

G

R

R

A

A

I

I

A

A

S

N

D

G

C

C

S

D

E

K

G

P

M

F

T

T

K

K

L

L

I

I

F

F

D

D

K

A

D

E

T

T

H

G

R

R

V

I

I

I

G

G

A

G

I

I

V

V

G

G

V

P

N

N

G

G

E

D

L

M

L

I

G

G

E

E

I

V

G

Y

L

L

A

A

I

I

E

E

M

M

G

G

C

C

D

D

A

A

E

A

D

D

L

I

A

G

L

K

T

T

I

I

H
|
H

A

P

H
|
H

P

P

T

T

L

L

H

G

E
|
E

S

S

V

I

G

G

L

M

A

A

E

E

I

V

Y

A

E

L

G

G

S

T

I

C

T

T

D

D

L

L

P

P

N
x
P

P
x
Q

K

K

active site: L41 (= L41), C45 (= C45), C50 (= C50), S53 (= S53), I195 (= I186), E199 (= E190), H454 (= H444), H456 (= H446), E461 (= E451), P479 (≠ N469), Q480 (≠ P470)
binding flavin-adenine dinucleotide: E36 (= E36), R37 (= R37), Y38 (≠ F38), G43 (= G43), V44 (= V44), C45 (= C45), G49 (= G49), C50 (= C50), K54 (= K54), D116 (≠ F116), G117 (= G117), Y135 (vs. gap), A156 (= A146), G157 (= G147), D324 (= D314), L331 (= L321), A332 (= A322)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 12, 16, 17

6uziC Crystal structure of dihydrolipoyl dehydrogenase from elizabethkingia anophelis nuhp1
44% identity, 95% coverage: 9:457/475 of query aligns to 9:459/470 of 6uziC

query
sites
6uziC

V

V

V

A

V

V

L
x
I

G
|
G

A

S

G
|
G

P
|
P

A
x
G

G

G

Y

Y

S

V

A

A

F

I

R

R

A

C

A

A

D

Q

L

L

G

G

L

F

E

K

T

T

V

V

I

I

V

I

E
|
E

R
x
K

F

Y

S

S

T

T

L

L

G

G

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

H

D

V

S

A

S

K

E

V

H

I

F

E

E

E

N

A

A

K

K

-

H

A

T

V

F

A

A

A

T

H

H

G

G

V

I

V

L

F

I

G

D

E

E

P

P

T

K

I

V

D

D

L

I

D

A

K

Q

L

M

R

I

S

S

F

R

K

K

Q

N

K

D

V

V

I

V

S

D

Q

Q

L

T

T

T

G

K

G

G

L

I

G

N

G

F

M

L

S

M

K

D

M

K

R

N

K

K

V

I

N

T

V

V

V

L

N

Q

G

G

F
x
V

G
|
G

K

S

F

F

S

E

G

S

P

A

N

T

S

Q

L

I

E

K

V

V

T

T

A

K

E

A

D

D

G

G

T

S

V

S

T

E

V

V

V

I

K

E

F

A

D

K

Q

N

A

T

I

I

A

A

A
x
T

G
|
G

S

S

R

K

P

P

I

S

K

S

L

L

P

P

F

F

I

I

P

T

H

L

E

D

D

K

P

E

R

R

I

V

W

I

D

T

S

S

T

T

D

E

A

A

L

L

E

N

L

L

K

K

E

E

V

V

P

P

G

K

K

H

L

L

L

I

V

V

M

I

G

G

I
x
V

I
|
I

G

G

L

L

E
|
E

M

L

G

G

T

S

V

V

Y

Y

S

L

S

R

L

L

G

G

S

S

E

D

I

V

D

T

V

V

E

E

M

Y

F

L

D

D

Q

K

V

I

I

I

P

P

A

G

A

M

D

D

K

G

D

T

V

L

V

S

R

K

V

E

F

L

T

Q

K

K

Q

T

I

L

K

K

K

Q

-

G

F

M

N

K

L

F

I

M

L

L

E

S

T

T

K

A

V

V

T

S

A

G

V

V

E

E

A

R

R

N

E

G

D

D

G

T

I

V

Y

K

V

V

S

T

M

A

E

K

G

D

K

K

S

K

A

G

P

E

A

D

E

V

P

V

V

V

R

E

Y

G

D

D

A

Y

V

C

L

L

V

V

A

S

I

V

G

G

R
|
R

T

R

P

P

N

Y

G

T

K

D

L

G

I

L

D

G

A

L

E

E

K

K

A

A

G

G

V

V

K

E

I

L

D

D

E

E

R

R

G

G

F

R

I

V

N

K

V

T

D

N

K

D

Q

H

L

L

R

Q

T

T

N

N

V

V

P

P

H

N

I

I

Y

Y

A

A

I

I

G

G

D
|
D

I

V

V

V

G

K

Q

G

P

A

M
|
M

L
|
L

A
|
A

H

H

K

K

G

A

V

E

H

E

E

E

G

G

H

V

V

F

A

V

A

A

E

E

V

T

I

L

A

A

G

G

M

E

K

K

H

P

Y

H

F

V

D

N

P

Y

K

N

V

L

I

I

P

P

S

G

I

V

A

V

Y

Y

T

T

D

W

P

P

E

E

V

V

A

A

W

G

V

V

G

G

L

K

T

T

E

E

K

E

E

Q

A

L

K

K

E

E

Q

A

G

G

I

V

A

A

Y

Y

E

K

T

T

A

G

T

S

F

F

P

P

W

M

A

R

A

A

S

L

G

G

R

R

A

S

I

R

A

A

S

S

D

M

C

D

S

T

E

D

G

G

M

V

T

I

K

K

L

I

I

L

F

A

D

D

K

E

D

K

T

T

H

D

R

E

V

I

I

L

G

G

G

V

A

H

I

M

V

I

G

G

V

A

N

R

G

A

E

D

L

M

L

I

G

A

E

E

I

A

G

V

L

V

A

A

I

M

E

E

M

F

G

R

C

A

D

S

A

A

E

E

D

D

L

I

A

A

L

R

T

I

I

S

H

H

A

A

H
|
H

P

P

T

T

L

Y

H

T

E
|
E

S

A

V

I

G

K

L

E

A

A

active site: C45 (= C45), C50 (= C50), S53 (= S53), V187 (≠ I186), E191 (= E190), H448 (= H446), E453 (= E451)
binding flavin-adenine dinucleotide: I12 (≠ L12), G13 (= G13), G15 (= G15), P16 (= P16), G17 (≠ A17), E36 (= E36), K37 (≠ R37), G43 (= G43), T44 (≠ V44), C45 (= C45), G49 (= G49), C50 (= C50), S53 (= S53), K54 (= K54), V117 (≠ F116), G118 (= G117), T147 (≠ A146), G148 (= G147), I188 (= I187), R276 (= R274), D316 (= D314), M322 (= M320), L323 (= L321), A324 (= A322)
binding zinc ion: H448 (= H446), E453 (= E451)

P11959 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; EC 1.8.1.4 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
45% identity, 97% coverage: 5:463/475 of query aligns to 8:463/470 of P11959

query
sites
P11959

I

I

K

E

T

T

Q

E

V

T

V

L

V

V

L

V

G

G

A

A

G

G

P

P

A

G

G

G

Y

Y

S

V

A

A

F

I

R

R

A

A

D

Q

L

L

G

G

L

Q

E

K

T

V

V

T

I

I

V

V

E
|
E

R
x
K

F

-

S
x
G

T
x
N

L
|
L

G
|
G

V
|
V

C
|
C

L

L

N

N

V

V

G

G

C

C

I

I

P

P

S

S

K
|
K

A

A

L

L

L

I

H

S

V

A

A

S

K

H

V

R

I

Y

E

E

E

Q

A

A

K

K

A

H

V

S

A

E

H

M

G

G

V

I

V

K

F

A

G

E

E

N

P

V

T

T

I

I

D

D

L

F

D

A

K

K

L

V

R

Q

S

E

F

W

K

K

Q

A

K

S

V

V

I

V

S

K

Q

K

L

L

T

T

G

G

L

V

G

E

G

G

M

L

S

L

K

K

M

G

R

N

K

K

V

V

N

E

V

I

V

V

N

K

G

G

F

E

G

A

K

Y

F

F

S

V

G

D

P

A

N

N

S

T

L

V

E

R

V

V

T

V

A

N

E

G

D

D

G

S

T

A

V

Q

T

T

V

Y

V

T

K

-

F

F

D

K

Q

N

A

A

I

I

A

A

A

T

G

G

S

S

R

R

P

P

I

I

K

E

L

L

P

P

F

N

I

F

P

K

H

F

E

S

D

N

P

-

R

R

I

I

W

L

D

D

S

S

T

T

D

G

A

A

L

L

E

N

L

L

K

G

E

E

V

V

P

P

G

K

K

S

L

L

L

V

V

V

M

I

G

G

I

Y

I

I

G

G

L

I

E

E

M

L

G

G

T

T

V

A

Y

Y

S

A

S

N

L

F

G

G

S

T

E

K

I

V

D

T

V

I

V

L

E

E

M

G

F

A

D

G

Q

E

V

I

I

L

P

S

A

G

A

F

D

E

K

K

D

Q

V

M

V

A

R

A

V

I

F

I

T

K

K

K

Q

R

I

L

K

K

-

G

F

V

N

E

L

V

I

V

L

T

E

N

T

A

K

L

V

A

T

K

A

G

V

A

E

E

A

E

R

R

E

E

D

D

G

G

I

V

Y

T

V

V

S

T

M

Y

E

E

G

A

K

-

S

N

A

G

P

E

A

T

E

K

P

T

V

I

R

D

Y

A

D

D

A

Y

V

V

L

L

V

V

A

T

I

V

G

G

R

R

T

R

P

P

N

N

G

T

K

D

L

E

I

L

D

G

A

L

E

E

K

Q

A

I

G

G

V

I

K

K

I

M

D

T

E

N

R

R

G

G

F

L

I

I

N

E

V

V

D

D

K

Q

Q

Q

L

C

R

R

T

T

N

S

V

V

P

P

H

N

I

I

Y

F

A

A

I

I

G

G

D
|
D

I

I

V

V

G

P

Q

G

P

P

M

A

L

L

A
|
A

H

H

K

K

G

A

V

S

H

Y

E

E

G

G

H

K

V

V

A

A

E

E

V

A

I

I

A

A

G

G

M

H

K

P

H

S

Y

A

F

V

D

D

P

Y

K

V

V

A

I

I

P

P

S

A

I

V

A

V

Y

F

T

S

D

D

P

P

E

E

V

C

A

A

W

S

V

V

G

G

L

Y

T

F

E

E

K

Q

E

Q

A

A

K

K

E

D

Q

E

G

G

I

I

A

D

Y

V

E

I

T

A

A

A

T

K

F

F

P

P

W

F

A

A

S

N

G

G

R

R

A

A

I

L

A

A

S

L

D

N

C

D

S

T

E

D

G

G

M

F

T

L

K

K

L

L

I

V

F

V

D

R

K

K

D

E

T

D

H

G

R

V

V

I

I

I

G

G

G

A

A

Q

I

I

V

I

G

G

V

P

N

N

G

A

G

S

E

D

L

M

L

I

G

A

E

E

I

L

G

G

L

L

A

A

I

I

E

E

M

A

G

G

C

M

D

T

A

A

E

E

D

D

L

I

A

A

L

L

T

T

I

I

H

H

A

A

H

H

P

P

T

T

L

L

H

G

E

E

S

I

V

A

G

M

L

E

A

A

E

E

I

V

Y

A

E

L

G

G

S

T

39:47 (vs. 36:45, 60% identical) binding
K56 (= K54) binding
D314 (= D314) binding
A322 (= A322) binding

1ebdA Dihydrolipoamide dehydrogenase complexed with the binding domain of the dihydrolipoamide acetylase (see paper)
45% identity, 96% coverage: 5:459/475 of query aligns to 2:453/455 of 1ebdA

query
sites
1ebdA

I

I

K

E

T

T

Q

E

V

T

V

L

V

V

L

V

G
|
G

A

A

G
|
G

P
|
P

A

G

G

G

Y

Y

S

V

A

A

F

I

R

R

A

A

D

Q

L

L

G

G

L

Q

E

K

T

V

V

T

I

I

V
|
V

E
|
E

R
x
K

F

-

S

G

T

N

L
|
L

G

G

G
|
G

V
|
V

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

L

I

H

S

V

A

A

S

K

H

V

R

I

Y

E

E

E

Q

A

A

K

K

A

H

V

S

A

E

H

M

G

G

V

I

V

K

F

A

G

E

E
x
N

P
x
V

T

T

I

I

D

D

L

F

D

A

K

K

L

V

R

Q

S

E

F

W

K

K

Q

A

K

S

V

V

I

V

S

K

Q

K

L

L

T

T

G

G

L

V

G

E

G

G

M

L

S

L

K

K

M

G

R

N

K

K

V

V

N

E

V

I

V

V

N

K

G

G

F
x
E

G
x
A

K

Y

F

F

S

V

G

D

P

A

N

N

S

T

L

V

E

R

V

V

T

V

A

N

E

G

D

D

G

S

T

A

V

Q

T

T

V

Y

V

T

K

-

F

F

D

K

Q

N

A

A

I

I

A

A

A
x
T

G
|
G

S

S

R

R

P

P

I

I

K

E

L

L

P

P

F

N

I

F

P

K

H

F

E

S

D

N

P

-

R

R

I

I

W

L

D

D

S

S

T

T

D

G

A

A

L

L

E

N

L

L

K

G

E

E

V

V

P

P

G

K

K

S

L

L

L

V

V

V

M

I

G

G

I
x
Y

I
|
I

G

G

L

I

E
|
E

M

L

G

G

T

T

V

A

Y

Y

S

A

S

N

L

F

G

G

S

T

E

K

I

V

D

T

V

I

V

L

E

E

M

G

F

A

D

G

Q

E

V

I

I

L

P

S

A

G

A

F

D

E

K

K

D

Q

V

M

V

A

R

A

V

I

F

I

T

K

K

K

Q

R

I

L

K

K

-

G

F

V

N

E

L

V

I

V

L

T

E

N

T

A

K

L

V

A

T

K

A

G

V

A

E

E

A

E

R

R

E

E

D

D

G

G

I

V

Y

T

V

V

S

T

M

Y

E

E

G

A

K

-

S

N

A

G

P

E

A

T

E

K

P

T

V

I

R

D

Y

A

D

D

A

Y

V

V

L

L

V

V

A

T

I

V

G

G

R
|
R

T

R

P

P

N

N

G

T

K

D

L

E

I

L

D

G

A

L

E

E

K

Q

A

I

G

G

V

I

K

K

I

M

D

T

E

N

R

R

G

G

F

L

I

I

N

E

V

V

D

D

K

Q

Q

Q

L

C

R

R

T

T

N

S

V

V

P

P

H

N

I

I

Y

F

A

A

I

I

G

G

D
|
D

I

I

V

V

G

P

Q

G

P

P

M
x
A

L
|
L

A
|
A

H

H

K

K

G

A

V
x
S

H

Y

E

E

G

G

H

K

V

V

A

A

E

E

V

A

I

I

A

A

G

G

M

H

K

P

H

S

Y

A

F

V

D

D

P

Y

K

V

V

A

I

I

P

P

S

A

I

V

A

V

Y

F

T

S

D

D

P

P

E

E

V

C

A

A

W

S

V

V

G

G

L

Y

T

F

E

E

K

Q

E

Q

A

A

K

K

E

D

Q

E

G

G

I

I

A

D

Y

V

E

I

T

A

A

A

T

K

F

F

P

P

W

F

A

A

S

N

G

G

R

R

A

A

I

L

A

A

S

L

D

N

C

D

S

T

E

D

G

G

M

F

T

L

K

K

L

L

I

V

F

V

D

R

K

K

D

E

T

D

H

G

R

V

V

I

I

I

G

G

G

A

A

Q

I

I

V

I

G

G

V

P

N

N

G

A

G

S

E

D

L

M

L

I

G

A

E

E

I

L

G

G

L

L

A

A

I

I

E

E

M

A

G

G

C

M

D

T

A

A

E

E

D

D

L

I

A

A

L

L

T

T

I

I

H
|
H

A

A

H
|
H

P

P

T

T

L

L

H

G

E
|
E

S

I

V

A

G

M

L

E

A

A

E

E

I

V

active site: P13 (= P16), L37 (= L41), C41 (= C45), C46 (= C50), S49 (= S53), N74 (≠ E78), V75 (≠ P79), Y180 (≠ I186), E184 (= E190), S320 (≠ V326), H438 (= H444), H440 (= H446), E445 (= E451)
binding flavin-adenine dinucleotide: G10 (= G13), G12 (= G15), P13 (= P16), V32 (= V35), E33 (= E36), K34 (≠ R37), G39 (= G43), V40 (= V44), C41 (= C45), G45 (= G49), C46 (= C50), K50 (= K54), E112 (≠ F116), A113 (≠ G117), T141 (≠ A146), G142 (= G147), Y180 (≠ I186), I181 (= I187), R268 (= R274), D308 (= D314), A314 (≠ M320), L315 (= L321), A316 (= A322)

P14218 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex; EC 1.8.1.4 from Pseudomonas fluorescens (see 2 papers)
42% identity, 99% coverage: 6:473/475 of query aligns to 4:478/478 of P14218

query
sites
P14218

K

K

T

F

Q

D

V

V

L

I

G

G

A

A

G

G

P

P

A

G

G

G

Y

Y

S

V

A

A

F

I

R

R

A

A

D

Q

L

L

G

G

L

L

E

K

T

T

V

A

I

C

V

I

E
|
E

R
x
K

F
x
Y

-
x
I

-
x
G

-
x
K

-
x
E

-
x
G

-
x
K

S
x
V

T
x
A

L
|
L

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G

G

C
|
C

I

I

P

P

S

S

K
|
K

A

A

L

L

H

D

V

S

A

S

K

Y

V

K

I

Y

E

H

E

E

A

A

K

K

-

E

A

A

V

F

A

K

A

V

H

H

G

G

V

I

V

E

F

A

G

K

E

G

P

V

T

T

I

I

D

D

L

V

D

P

K

A

L

M

R

V

S

A

F

R

K

K

Q

A

K

N

V

I

I

V

S

K

Q

N

L

L

T

T

G

G

L

I

G

A

G

T

M

L

S

F

K

K

M

A

R

N

K

G

V

V

N

T

V

S

V

F

N

E

G

G

F

H

G
|
G

K

K

F

L

S

L

G

A

P

N

N

K

S

Q

L

V

E

E

V

V

T

T

A

G

E

L

D

D

G

G

T

K

V

T

T

Q

V

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V

L

K

E

F

A

D

E

Q

N

A

V

I

I

A

A

A

S

G

G

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S

R

R

P

P

I

V

K

E

L

I

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F

P

I

A

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L

E

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D

P

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R

I

I

I

W

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D

D

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T

D

G

A

A

L

L

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E

L

F

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Q

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A

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M

L

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A

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M

A

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D

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K

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F

I

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P

A

A

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D

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E

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-

I

-

A

K

K

D

E

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A

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R

K

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F

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T

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Q

I

-

K

-

K

G

F

L

N

N

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I

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N

A

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A

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-

Y

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A

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V

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L

I

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A

A

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N

V

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T

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A

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A

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T

I

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F

A

A

I

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D
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D

I

V

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V

G

R

Q

G

P

A

M

M

L

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A
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A

H

H

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K

G

A

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H

E

E

E

G

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H

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A

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I

I

A

A

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A

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I

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A

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V

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T

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L

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A

Q

E

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Y

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T

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A

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N

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A

G

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M

F

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G

C

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A

A

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D

D

L

L

A

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L

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T

M

I

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F

A

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H

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P

P

T

T

L

L

H

S

E

E

S

A

V

L

G

H

L

E

A

A

E

L

I

A

Y

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E

N

G

G

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D

H

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I

P

A

N

N

P

R

K

K

A

K

K

R

34:49 (vs. 36:45, 31% identical) binding
C49 (= C45) modified: Disulfide link with 54, Redox-active
C54 (= C50) modified: Disulfide link with 49, Redox-active
K58 (= K54) binding
G122 (= G117) binding
D319 (= D314) binding
A327 (= A322) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

5u8vA Dihydrolipoamide dehydrogenase (lpdg) from pseudomonas aeruginosa bound to NAD+ (see paper)
43% identity, 98% coverage: 6:469/475 of query aligns to 2:472/472 of 5u8vA

query
sites
5u8vA

K

K

T

F

Q

D

V

V

L
x
I

G

G

A

A

G
|
G

P
|
P

A
x
G

G

G

Y

Y

S

V

A

A

F

I

R

R

A

A

D

Q

L

L

G

G

L

L

E

K

T

T

V

A

I

C

V

I

E
|
E

R

K

F

Y

-

I

-

G

-

K

-

E

-

G

-

K

S

V

T

A

L
|
L

G

G

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

H

D

V

S

A

S

K

Y

V

K

I

Y

E

H

E

E

A

A

K

K

-

E

A

A

V

F

A

K

A

V

H

H

G

G

V

I

V

E

F

A

G

K

E
x
G

P
x
V

T

T

I

I

D

D

L

V

D

P

K

A

L

M

R

V

S

A

F

R

K

K

Q

A

K

N

V

I

I

V

S

K

Q

N

L

L

T

T

G

G

L

I

G

A

G

T

M

L

S

F

K

K

M

A

R

N

K

G

V

V

N

T

V

S

V

F

N

E

G

G

F
x
H

G
|
G

K

K

F

L

S

L

G

A

P

N

N

K

S

Q

L

V

E

E

V

V

T

T

A

G

E

L

D

D

G

G

T

K

V

T

T

Q

V

V

V

L

K

E

F

A

D

E

Q

N

A

V

I

I

A
|
A

A
x
S

G
|
G

S

S

R

R

P

P

I

V

K

E

L

I

P

P

F

P

I

A

P

P

H

L

E

T

D

D

P

D

R

I

I

I

W

V

D

D

S
|
S

T

T

D

G

A

A

L

L

E

E

L

F

K

Q

E

A

V

V

P

P

G

K

K

K

L

L

L

G

V

V

M
x
I

G
|
G

G

A

G
|
G

I
x
V

I
|
I

G

G

L

L

E
|
E

M

L

G

G

T

S

V

V

Y

W

S

A

S

R

L

L

G

G

S

A

E

E

I

V

D

T

V

V

V
x
L

E
|
E

M
x
A

F

L

D

D

Q

K

V

F

I

L

P

P

A

A

D

D

-

E

-

Q

-

I

-

A

K

K

D

E

V

A

V

L

R

K

V

V

F

L

T

T

K

K

Q

Q

I

-

K

-

K

G

F

L

N

N

L

I

I

R

L

L

E

G

T

A

K

R

V
|
V

T

T

A

A

V

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E

E

A

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R

K

E

K

D

K

G

Q

I

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Y

T

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M

F

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T

G

D

K

A

S

N

A

G

P

E

A

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P

E

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T

R

-

Y

F

D

D

A

K

V

L

L

I

V

V

A

A

I
x
V

G
|
G

R
|
R

T

R

P

P

N

V

G

T

K

T

L

D

I

L

D

L

A

A

E

A

K

D

A

S

G

G

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V

K

T

I

L

D

D

E

E

R

R

G

G

F

F

I

I

N

Y

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D

D

K

D

Q

H

L

C

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K

T

T

N

S

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P

H

G

I

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Y

F

A

A

I

I

G
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G

D
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D

I

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V

G

R

Q

G

P

A

M
|
M

L
|
L

A
|
A

H
|
H

K

K

G

A

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x
S

H

E

E

E

G

G

H

V

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M

A

V

A

A

E

E

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I

I

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A

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Y

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P

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I

A

A

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W

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V

G

G

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T

E

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Q

E

T

A

L

K

K

E

A

Q

E

G

G

I

V

A

E

Y

V

E

N

T

V

A

G

T

T

F

F

P

P

W

F

A

A

S

S

G

G

R

R

A

A

I

M

A

A

S

A

D

N

C

D

S

T

E

T

G

G

M

L

T

V

K

K

L

V

I

I

F

A

D

D

K

A

D

K

T

T

H

D

R

R

V

V

I

L

G

G

G

V

A

H

I

V

V

I

G

G

V

P

N

S

G

A

E

E

L

L

L

V

G

Q

E

Q

I

G

G

A

L

I

A

G

I

M

E

E

M

F

G

G

C

T

D

S

A

A

E

E

D

D

L

L

A

G

L

M

T

M

I

V

H
x
F

A

S

H
|
H

P
|
P

T

T

L

L

H

S

E
|
E

S

A

V

L

G

H

L

E

A

A

E

L

I

A

Y

V

E

N

G

G

S

H

I

A

T

I

D

H

L

I

P

A

N
|
N

active site: P12 (= P16), L43 (= L41), C47 (= C45), C52 (= C50), S55 (= S53), G81 (≠ E78), V82 (≠ P79), V189 (≠ I186), E193 (= E190), S329 (≠ V326), F447 (≠ H444), H449 (= H446), E454 (= E451), N472 (= N469)
binding flavin-adenine dinucleotide: I8 (≠ L12), G11 (= G15), P12 (= P16), G13 (≠ A17), E32 (= E36), G45 (= G43), T46 (≠ V44), C47 (= C45), G51 (= G49), C52 (= C50), K56 (= K54), H119 (≠ F116), G120 (= G117), A148 (= A145), S149 (≠ A146), G150 (= G147), S169 (= S166), I190 (= I187), R277 (= R274), G316 (= G313), D317 (= D314), M323 (= M320), L324 (= L321), A325 (= A322), H326 (= H323), H449 (= H446), P450 (= P447)
binding nicotinamide-adenine-dinucleotide: I185 (≠ M182), G186 (= G183), G188 (= G185), V189 (≠ I186), I190 (= I187), L208 (≠ V205), E209 (= E206), A210 (≠ M207), V243 (= V239), V275 (≠ I272), G276 (= G273)

5u8wA Dihydrolipoamide dehydrogenase (lpdg) from pseudomonas aeruginosa bound to nadh (see paper)
43% identity, 98% coverage: 6:469/475 of query aligns to 3:473/473 of 5u8wA

query
sites
5u8wA

K

K

T

F

Q

D

V

V

L
x
I

G

G

A

A

G
|
G

P
|
P

A
x
G

G

G

Y

Y

S

V

A

A

F

I

R

R

A

A

D

Q

L

L

G

G

L

L

E

K

T

T

V

A

I

C

V

I

E
|
E

R
x
K

F

Y

-

I

-

G

-

K

-

E

-

G

-

K

S

V

T

A

L
|
L

G

G

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

H

D

V

S

A

S

K

Y

V

K

I

Y

E

H

E

E

A

A

K

K

-

E

A

A

V

F

A

K

A

V

H

H

G

G

V

I

V

E

F

A

G

K

E
x
G

P
x
V

T

T

I

I

D

D

L

V

D

P

K

A

L

M

R

V

S

A

F

R

K

K

Q

A

K

N

V

I

I

V

S

K

Q

N

L

L

T

T

G

G

L

I

G

A

G

T

M

L

S

F

K

K

M

A

R

N

K

G

V

V

N

T

V

S

V

F

N

E

G

G

F
x
H

G
|
G

K

K

F

L

S

L

G

A

P

N

N

K

S

Q

L

V

E

E

V

V

T

T

A

G

E

L

D

D

G

G

T

K

V

T

T

Q

V

V

V

L

K

E

F

A

D

E

Q

N

A

V

I

I

A
|
A

A
x
S

G
|
G

S

S

R

R

P

P

I

V

K

E

L

I

P

P

F

P

I

A

P

P

H

L

E

T

D

D

P

D

R

I

I

I

W

V

D

D

S
|
S

T

T

D

G

A

A

L

L

E

E

L

F

K

Q

E

A

V

V

P

P

G

K

K

K

L

L

L

G

V

V

M
x
I

G

G

G

A

G
|
G

I
x
V

I
|
I

G

G

L

L

E
|
E

M

L

G

G

T

S

V

V

Y

W

S

A

S

R

L

L

G

G

S

A

E

E

I

V

D

T

V

V

V

L

E
|
E

M
x
A

F
x
L

D

D

Q

K

V

F

I

L

P

P

A

A

D

D

-

E

-

Q

-

I

-

A

K

K

D

E

V

A

V

L

R

K

V

V

F

L

T

T

K

K

Q

Q

I

-

K

-

K

G

F

L

N

N

L

I

I

R

L

L

E

G

T

A

K

R

V

V

T

T

A

A

V

S

E

E

A

V

R

K

E

K

D

K

G

Q

I

V

Y

T

V

V

S

T

M

F

E

T

G

D

K

A

S

N

A

G

P

E

A

Q

E

K

P

E

V

T

R

-

Y

F

D

D

A

K

V

L

L

I

V

V

A
|
A

I
x
V

G
|
G

R
|
R

T

R

P

P

N

V

G

T

K

T

L

D

I

L

D

L

A

A

E

A

K

D

A

S

G

G

V

V

K

T

I

L

D

D

E

E

R

R

G

G

F

F

I

I

N

Y

V

V

D

D

K

D

Q

H

L

C

R

K

T

T

N

S

V

V

P

P

H

G

I

V

Y

F

A

A

I

I

G
|
G

D
|
D

I

V

V

V

G

R

Q

G

P

A

M
|
M

L
|
L

A
|
A

H
|
H

K

K

G

A

V
x
S

H

E

E

E

G

G

H

V

V

M

A

V

A

A

E

E

V

R

I

I

A

A

G

G

M

H

K

K

H

A

Y

Q

F

M

D

N

P

Y

K

D

V

L

I

I

P

P

S

S

I
x
V

A

I

Y
|
Y

T

T

D

H

P

P

E

E

V

I

A

A

W

W

V

V

G

G

L

K

T

T

E

E

K

Q

E

T

A

L

K

K

E

A

Q

E

G

G

I

V

A

E

Y

V

E

N

T

V

A

G

T

T

F

F

P

P

W

F

A

A

S

S

G

G

R

R

A

A

I

M

A

A

S

A

D

N

C

D

S

T

E

T

G

G

M

L

T

V

K

K

L

V

I

I

F

A

D

D

K

A

D

K

T

T

H

D

R

R

V

V

I

L

G

G

G

V

A

H

I

V

V

I

G

G

V

P

N

S

G

A

E

E

L

L

L

V

G

Q

E

Q

I

G

G

A

L

I

A

G

I

M

E

E

M

F

G

G

C

T

D

S

A

A

E

E

D

D

L

L

A

G

L

M

T

M

I

V

H
x
F

A

S

H
|
H

P
|
P

T

T

L

L

H

S

E
|
E

S

A

V

L

G

H

L

E

A

A

E

L

I

A

Y

V

E

N

G

G

S

H

I

A

T

I

D

H

L

I

P

A

N
|
N

active site: P13 (= P16), L44 (= L41), C48 (= C45), C53 (= C50), S56 (= S53), G82 (≠ E78), V83 (≠ P79), V190 (≠ I186), E194 (= E190), S330 (≠ V326), F448 (≠ H444), H450 (= H446), E455 (= E451), N473 (= N469)
binding flavin-adenine dinucleotide: I9 (≠ L12), G12 (= G15), P13 (= P16), G14 (≠ A17), E33 (= E36), K34 (≠ R37), G46 (= G43), T47 (≠ V44), C48 (= C45), G52 (= G49), C53 (= C50), K57 (= K54), H120 (≠ F116), G121 (= G117), A149 (= A145), S150 (≠ A146), G151 (= G147), S170 (= S166), G317 (= G313), D318 (= D314), M324 (= M320), L325 (= L321), A326 (= A322), H327 (= H323), Y357 (= Y353), H450 (= H446), P451 (= P447)
binding 1,4-dihydronicotinamide adenine dinucleotide: I186 (≠ M182), G189 (= G185), V190 (≠ I186), I191 (= I187), E194 (= E190), E210 (= E206), A211 (≠ M207), L212 (≠ F208), A275 (= A271), V276 (≠ I272), G277 (= G273), R278 (= R274), M324 (= M320), L325 (= L321), V355 (≠ I351), Y357 (= Y353)

5u8uD Dihydrolipoamide dehydrogenase (lpdg) from pseudomonas aeruginosa (see paper)
43% identity, 98% coverage: 6:469/475 of query aligns to 6:476/477 of 5u8uD

query
sites
5u8uD

K

K

T

F

Q

D

V

V

L
x
I

G

G

A

A

G
|
G

P
|
P

A
x
G

G

G

Y

Y

S

V

A

A

F

I

R

R

A

A

D

Q

L

L

G

G

L

L

E

K

T

T

V

A

I

C

V

I

E
|
E

R
x
K

F

Y

-

I

-

G

-

K

-

E

-

G

-

K

S

V

T

A

L
|
L

G

G

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

H

D

V

S

A

S

K

Y

V

K

I

Y

E

H

E

E

A

A

K

K

-

E

A

A

V

F

A

K

A

V

H

H

G

G

V

I

V

E

F

A

G

K

E
x
G

P
x
V

T

T

I

I

D

D

L

V

D

P

K

A

L

M

R

V

S

A

F

R

K

K

Q

A

K

N

V

I

I

V

S

K

Q

N

L

L

T

T

G

G

L

I

G

A

G

T

M

L

S

F

K

K

M

A

R

N

K

G

V

V

N

T

V

S

V

F

N

E

G

G

F
x
H

G
|
G

K

K

F

L

S

L

G

A

P

N

N

K

S

Q

L

V

E

E

V

V

T

T

A

G

E

L

D

D

G

G

T

K

V

T

T

Q

V

V

V

L

K

E

F

A

D

E

Q

N

A

V

I

I

A
|
A

A
x
S

G
|
G

S

S

R

R

P

P

I

V

K

E

L

I

P

P

F

P

I

A

P

P

H

L

E

T

D

D

P

D

R

I

I

I

W

V

D

D

S

S

T

T

D

G

A

A

L

L

E

E

L

F

K

Q

E

A

V

V

P

P

G

K

K

K

L

L

L

G

V

V

M

I

G

G

G

A

G

G

I
x
V

I
|
I

G

G

L

L

E
|
E

M

L

G

G

T

S

V

V

Y

W

S

A

S

R

L

L

G

G

S

A

E

E

I

V

D

T

V

V

V

L

E

E

M

A

F

L

D

D

Q

K

V

F

I

L

P

P

A

A

D

D

-

E

-

Q

-

I

-

A

K

K

D

E

V

A

V

L

R

K

V

V

F

L

T

T

K

K

Q

Q

I

-

K

-

K

G

F

L

N

N

L

I

I

R

L

L

E

G

T

A

K

R

V

V

T

T

A

A

V

S

E

E

A

V

R

K

E

K

D

K

G

Q

I

V

Y

T

V

V

S

T

M

F

E

T

G

D

K

A

S

N

A

G

P

E

A

Q

E

K

P

E

V

T

R

-

Y

F

D

D

A

K

V

L

L

I

V

V

A

A

I

V

G

G

R
|
R

T

R

P

P

N

V

G

T

K

T

L

D

I

L

D

L

A

A

E

A

K

D

A

S

G

G

V

V

K

T

I

L

D

D

E

E

R

R

G

G

F

F

I

I

N

Y

V

V

D

D

K

D

Q

H

L

C

R

K

T

T

N

S

V

V

P

P

H

G

I

V

Y

F

A

A

I

I

G
|
G

D
|
D

I

V

V

V

G

R

Q

G

P

A

M
|
M

L
|
L

A
|
A

H
|
H

K

K

G

A

V
x
S

H

E

E

E

G

G

H

V

V

M

A

V

A

A

E

E

V

R

I

I

A

A

G

G

M

H

K

K

H

A

Y

Q

F

M

D

N

P

Y

K

D

V

L

I

I

P

P

S

S

I

V

A

I

Y

Y

T

T

D

H

P

P

E

E

V

I

A

A

W

W

V

V

G

G

L

K

T

T

E

E

K

Q

E

T

A

L

K

K

E

A

Q

E

G

G

I

V

A

E

Y

V

E

N

T

V

A

G

T

T

F

F

P

P

W

F

A

A

S

S

G

G

R

R

A

A

I

M

A

A

S

A

D

N

C

D

S

T

E

T

G

G

M

L

T

V

K

K

L

V

I

I

F

A

D

D

K

A

D

K

T

T

H

D

R

R

V

V

I

L

G

G

G

V

A

H

I

V

V

I

G

G

V

P

N

S

G

A

E

E

L

L

L

V

G

Q

E

Q

I

G

G

A

L

I

A

G

I

M

E

E

M

F

G

G

C

T

D

S

A

A

E

E

D

D

L

L

A

G

L

M

T

M

I

V

H
x
F

A

S

H
|
H

P
|
P

T

T

L

L

H

S

E
|
E

S

A

V

L

G

H

L

E

A

A

E

L

I

A

Y

V

E

N

G

G

S

H

I

A

T

I

D

H

L

I

P

A

N
|
N

active site: P16 (= P16), L47 (= L41), C51 (= C45), C56 (= C50), S59 (= S53), G85 (≠ E78), V86 (≠ P79), V193 (≠ I186), E197 (= E190), S333 (≠ V326), F451 (≠ H444), H453 (= H446), E458 (= E451), N476 (= N469)
binding flavin-adenine dinucleotide: I12 (≠ L12), G15 (= G15), P16 (= P16), G17 (≠ A17), E36 (= E36), K37 (≠ R37), G49 (= G43), T50 (≠ V44), C51 (= C45), G55 (= G49), C56 (= C50), K60 (= K54), H123 (≠ F116), G124 (= G117), A152 (= A145), S153 (≠ A146), G154 (= G147), I194 (= I187), R281 (= R274), G320 (= G313), D321 (= D314), M327 (= M320), L328 (= L321), A329 (= A322), H330 (= H323), H453 (= H446), P454 (= P447)

Sites not aligning to the query:

active site: 477

P18925 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; EC 1.8.1.4 from Azotobacter vinelandii (see 2 papers)
41% identity, 98% coverage: 6:471/475 of query aligns to 4:476/477 of P18925

query
sites
P18925

K

K

T

F

Q

D

V

V

V

I

V

V

L

I

G

G

A

A

G

G

P

P

A

G

G

G

Y

Y

S

V

A

A

F

I

R

K

A

S

A

A

D

Q

L

L

G

G

L

L

E

K

T

T

V

A

I

L

V

I

E
|
E

R
x
K

F
x
Y

-
x
K

-
x
G

-
x
K

-
x
E

-
x
G

-
x
K

S
x
T

T
x
A

L
|
L

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G

G

C
|
C

I

I

P

P

S

S

K
|
K

A

A

L

L

H

D

V

S

A

S

K

Y

V

K

I

F

E

H

E

E

A

A

-

H

K

E

A

S

V

F

A

K

A

L

H

H

G

G

V

I

V

S

F

T

G

G

E

E

P

V

T

A

I

I

D

D

L

V

D

P

K

T

L

M

R

I

S

A

F

R

K

K

Q

D

K

Q

V

I

I

V

S

R

Q

N

L

L

T

T

G

G

L

V

G

A

G

S

M

L

S

I

K

K

M

A

R

N

K

G

V

V

N

T

V

L

V

F

N

E

G

G

F

H

G

G

K

K

F

L

S

L

G

A

P

G

N

K

S

K

L

V

E

E

V

V

T

T

A

A

E

A

D

D

G

G

T

S

V

S

T

Q

V

V

V

L

K

D

F

T

D

E

Q

N

A

V

I

I

I

L

A

A

A

S

G

G

S

S

R

K

P

P

I

V

K

E

L

I

P

P

F

P

I

A

P

P

H

V

E

D

D

Q

P

D

R

V

I

I

W

V

D

D

S

S

T

T

D

G

A

A

L

L

E

D

L

F

K

Q

E

N

V

V

P

P

G

G

K

K

L

L

L

G

V

V

M

I

G

G

G

A

G

G

I

V

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34:49 (vs. 36:45, 31% identical) binding
C49 (= C45) modified: Disulfide link with 54, Redox-active
C54 (= C50) modified: Disulfide link with 49, Redox-active
K58 (= K54) binding
D319 (= D314) binding
A327 (= A322) binding

6hg8B Crystal structure of the r460g disease-causing mutant of the human dihydrolipoamide dehydrogenase.
43% identity, 95% coverage: 5:456/475 of query aligns to 13:470/482 of 6hg8B

query
sites
6hg8B

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active site: C53 (= C45), C58 (= C50), S61 (= S53), V196 (≠ I186), E200 (= E190), H460 (= H446), E465 (= E451)
binding flavin-adenine dinucleotide: I20 (≠ L12), G23 (= G15), P24 (= P16), G25 (≠ A17), E44 (= E36), K45 (≠ R37), N46 (≠ F38), G51 (= G43), T52 (≠ V44), C53 (= C45), G57 (= G49), C58 (= C50), K62 (= K54), Y126 (≠ F116), G127 (= G117), T156 (≠ A146), G157 (= G147), I197 (= I187), R288 (= R274), F291 (≠ N277), G327 (= G313), D328 (= D314), M334 (= M320), L335 (= L321), A336 (= A322), H337 (= H323)

P09622 Dihydrolipoyl dehydrogenase, mitochondrial; Dihydrolipoamide dehydrogenase; Glycine cleavage system L protein; EC 1.8.1.4 from Homo sapiens (Human) (see 14 papers)
43% identity, 94% coverage: 5:452/475 of query aligns to 40:493/509 of P09622

query
sites
P09622

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71:80 (vs. 36:45, 60% identical) binding
K72 (≠ R37) to E: in DLDD; reduced dihydrolipoyl dehydrogenase activity; no effect on interaction with PDHX; dbSNP:rs121964987
K89 (= K54) binding ; mutation to E: Abolishes dihydrolipoyl dehydrogenase activity. Does not affect interaction with PDHX.
K104 (= K67) to T: in dbSNP:rs1130477
G154 (= G117) binding
TGS 183:185 (≠ AGS 146:148) binding
220:227 (vs. 183:190, 63% identical) binding
E243 (= E206) binding
V278 (= V239) binding
G314 (= G273) binding
D355 (= D314) binding
MLAH 361:364 (= MLAH 320:323) binding
E375 (= E334) to K: in DLDD; loss of enzyme activity; abolished interaction with PDHX; dbSNP:rs121964992
H383 (≠ Y342) mutation to A: Reduces dihydrolipoyl dehydrogenase activity.; mutation to L: Reduces dihydrolipoyl dehydrogenase activity.
D448 (≠ H407) Important for interaction with PDHX and activity of multienzyme pyruvate dehydrogenase complex; mutation to A: Reduces interaction with PDHX. Inhibits multienzyme pyruvate dehydrogenase complex activity. Does not affect dihydrolipoyl dehydrogenase activity.; mutation to N: Does not affect dihydrolipoyl dehydrogenase activity.
E466 (= E425) mutation to A: Decreases dehydrogenase activity. Loss of proteolytic activity.
Y473 (≠ M432) Important for interaction with PDHX and activity of multienzyme pyruvate dehydrogenase complex; mutation to A: Reduces interaction with PDHX. Inhibits multienzyme pyruvate dehydrogenase complex activity. Does not affect dihydrolipoyl dehydrogenase activity.; mutation to F: Does not affect dihydrolipoyl dehydrogenase activity.; mutation to H: Reduces interaction with PDHX. Inhibits multienzyme pyruvate dehydrogenase complex activity. Does not affect dihydrolipoyl dehydrogenase activity.
D479 (= D438) to V: in DLDD; reduced dehydrogenase activity; increased proteolytic activity; dbSNP:rs397514649
R482 (≠ L441) to G: in DLDD; reduced enzyme activity; dbSNP:rs397514650; mutation to A: Does not affect dihydrolipoyl dehydrogenase activity.; mutation to M: Does not affect interaction with PDHX.
H485 (= H444) mutation to A: Loss of dehydrogenase activity. Increases proteolytic activity.
P488 (= P447) to L: in DLDD; no effect on interaction with PDHX; dbSNP:rs121964988
S491 (≠ H450) mutation to A: Loss of proteolytic activity. Does not affect dehydrogenase activity.
E492 (= E451) mutation to Q: Reduces dihydrolipoyl dehydrogenase activity. Does not affect interaction with PDHX.

Sites not aligning to the query:

495 R → G: in DLDD; loss of enzyme activity; reduced interaction with PDHX; dbSNP:rs121964989
505 K→M: Reduces dihydrolipoyl dehydrogenase activity. Does not affect interaction with PDHX.

3ladA Refined crystal structure of lipoamide dehydrogenase from azotobacter vinelandii at 2.2 angstroms resolution. A comparison with the structure of glutathione reductase (see paper)
42% identity, 95% coverage: 6:457/475 of query aligns to 3:461/472 of 3ladA

query
sites
3ladA

K

K

T

F

Q

D

V

V

V

I

V

V

L
x
I

G
|
G

A

A

G
|
G

P
|
P

A

G

G

G

Y

Y

S

V

A

A

F

I

R

K

A

S

A

A

D

Q

L

L

G

G

L

L

E

K

T

T

V

A

I

L

V

I

E
|
E

R
x
K

F

Y

-

K

-

G

-

K

-

E

-

G

-

K

S

T

T

A

L
|
L

G

G

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K

K

A

A

L

L

H

D

V

S

A

S

K

Y

V

K

I

F

E

H

E

E

A

A

-

H

K

E

A

S

V

F

A

K

A

L

H

H

G

G

V

I

V

S

F

T

G

G

E

E

P

V

T

A

I

I

D

D

L

V

D

P

K

T

L

M

R

I

S

A

F

R

K

K

Q

D

K

Q

V

I

I

V

S

R

Q

N

L

L

T

T

G

G

L

V

G

A

G

S

M

L

S

I

K

K

M

A

R

N

K

G

V

V

N

T

V

L

V

F

N

E

G

G

F
x
H

G
|
G

K

K

F

L

S

L

G

A

P

G

N

K

S

K

L

V

E

E

V

V

T

T

A

A

E

A

D

D

G

G

T

S

V

S

T

Q

V

V

V

L

K

D

F

T

D

E

Q

N

A

V

I

I

I

L

A
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A

A
x
S

G
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G

S

S

R

K

P

P

I

V

K

E

L

I

P

P

F

P

I

A

P

P

H

V

E

D

D

Q

P

D

R

V

I

I

W

V

D

D

S

S

T

T

D

G

A

A

L

L

E

D

L

F

K

Q

E

N

V

V

P

P

G

G

K

K

L

L

L

G

V

V

M

I

G

G

G

A

G

G

I
x
V

I
|
I

G

G

L

L

E
|
E

M

L

G

G

T

S

V

V

Y

W

S

A

S

R

L

L

G

G

S

A

E

E

I

V

D

T

V

V

V

L

E

E

M

A

F

M

D

D

Q

K

V

F

I

L

P

P

A

A

A

V

D

D

K

E

D

Q

V

V

V

A

R

K

V

E

F

A

T

Q

K

K

Q

I

I

L

K

T

K

K

K

Q

-

G

F

L

N

K

L

I

I

L

L

L

E

G

T

A

K

R

V

V

T

T

A

G

V

T

E

E

A

V

R

K

E

N

D

K

G

Q

I

V

-

T

-

V

-

K

Y

F

V

V

S

D

M

A

E

E

G

G

K

E

S

K

A

S

P

Q

A

A

E

-

P

-

V

-

R

-

Y

F

D

D

A

K

V

L

L

I

V

V

A

A

I

V

G

G

R
|
R

T

R

P

P

N

V

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T

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D

I

L

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L

A

A

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A

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D

A

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G

G

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V

K

T

I

L

D

D

E

E

R

R

G

G

F

F

I

I

N

Y

V

V

D

D

K

D

Q

Y

L

C

R

A

T

T

N

S

V

V

P

P

H

G

I

V

Y

Y

A

A

I

I

G

G

D
|
D

I

V

V

V

G

R

Q

G

P

A

M

M

L
|
L

A
|
A

H

H

K

K

G

A

V

S

H

E

E

E

G

G

H

V

V

V

A

V

A

A

E

E

V

R

I

I

A

A

G

G

M

H

K

K

H

A

Y

Q

F

M

D

N

P

Y

K

D

V

L

I

I

P

P

S

A

I

V

A

I

Y

Y

T

T

D

H

P

P

E

E

V

I

A

A

W

G

V

V

G

G

L

K

T

T

E

E

K

Q

E

A

A

L

K

K

E

A

Q

E

G

G

I

V

A

A

Y

I

E

N

T

V

A

G

T

V

F

F

P

P

W

F

A

A

S

S

G

G

R

R

A

A

I

M

A

A

S

A

D

N

C

D

S

T

E

A

G

G

M

F

T

V

K

K

L

V

I

I

F

A

D

D

K

A

D

K

T

T

H

D

R

R

V

V

I

L

G

G

G

V

A

H

I

V

V

I

G

G

V

P

N

S

G

A

E

E

L

L

L

V

G

Q

E

Q

I

G

G

A

L

I

A

A

I

M

E

E

M

F

G

G

C

T

D

S

A

A

E

E

D

D

L

L

A

G

L

M

T

M

I

V

H
x
F

A

A

H
|
H

P

P

T

A

L

L

H

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E
|
E

S

A

V

L

G

H

L

E

A

A

active site: L44 (= L41), C48 (= C45), C53 (= C50), S56 (= S53), V190 (≠ I186), E194 (= E190), F448 (≠ H444), H450 (= H446), E455 (= E451)
binding flavin-adenine dinucleotide: I9 (≠ L12), G10 (= G13), G12 (= G15), P13 (= P16), E33 (= E36), K34 (≠ R37), G46 (= G43), T47 (≠ V44), C48 (= C45), G52 (= G49), C53 (= C50), H120 (≠ F116), G121 (= G117), A149 (= A145), S150 (≠ A146), G151 (= G147), I191 (= I187), R278 (= R274), D318 (= D314), L325 (= L321), A326 (= A322)

1zmdA Crystal structure of human dihydrolipoamide dehydrogenase complexed to nadh (see paper)
43% identity, 94% coverage: 5:452/475 of query aligns to 3:456/472 of 1zmdA

query
sites
1zmdA

I

I

K

D

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A

Q

D

V

V

V

T

V

V

L
x
I

G
|
G

A

S

G
|
G

P
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P

A
x
G

G

G

Y

Y

S

V

A

A

F

I

R

K

A

A

D

Q

L

L

G

G

L

F

E

K

T

T

V

V

I

C

V

I

E
|
E

R
x
K

F
x
N

S

E

T

T

L
|
L

G

G

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

H

N

V

N

A

S

K

H

V

Y

I

Y

E

H

E

M

A

A

K

H

A

G

V

T

-

D

-

F

A

A

A

S

H

R

G

G

V

I

V

E

F

M

G

S

E

E

P

V

T

R

I

L

D

N

L

L

D

D

K

K

L

M

R

M

S

E

F

Q

K

K

Q

S

K

T

V

A

I

V

S

K

Q

A

L

L

T

T

G

G

L

I

G

A

G

H

M

L

S

F

K

K

M

Q

R

N

K

K

V

V

N

V

V

H

V

V

N

N

G

G

F
x
Y

G
|
G

K

K

F

I

S

T

G

G

P

K

N

N

S

Q

L

V

E

T

V

A

T

T

A

K

E

A

D

D

G

G

T

G

V

T

T

Q

V

V

V

I

K

D

F

T

D

K

Q

N

A

I

I

L

I

I

A

A

A
x
T

G
|
G

S

S

R

E

P

V

I

T

K

P

L

F

P

P

F

G

I

I

P

T

H

I

E

D

D

E

P

D

R

T

I

I

W

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D

S

S
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S

T

T

D

G

A

A

L

L

E

S

L

L

K

K

E

K

V

V

P

P

G

E

K

K

L

M

L

V

V

V

M
x
I

G
|
G

G

A

G
|
G

I
x
V

I
|
I

G

G

L

V

E
|
E

M

L

G

G

T

S

V

V

Y

W

S

Q

S

R

L

L

G

G

S

A

E

D

I

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T

V

A

V

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|
E

M
x
F

F
x
L

D

G

Q

H

V

V

I

G

P

G

A

V

A

G

-

I

D

D

K

M

D

E

V

I

V

S

R

K

V

N

F

F

T

Q

K

R

Q

I

I

L

K

Q

K

K

K

Q

-

G

F

F

N

K

L

F

I

K

L

L

E

N

T

T

K

K

V

V

T

T

A

G

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A

E

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D

G

G

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K

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Y

D

V

V

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M

I

E

E

G

A

K

A

S

S

A

G

-

G

P

K

A

A

E

E

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Y

C

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D

A

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L

L

V

V

A

C

I
|
I

G
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G

R
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R

T

R

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P

N
x
F

G

T

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K

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N

I

L

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A

L

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E

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E

A

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E

I

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E

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F

R

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I

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N

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N

I

I

Y

Y

A

A

I

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G
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G

D
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D

I

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A

Q

G

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P

M
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M

L
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L

A
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A

H
|
H

K

K

G

A

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E

H

D

E

E

G

G

H

I

V

I

A

C

A

V

E

E

V

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I

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A

A

G

G

M

G

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A

H

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Y

H

F

I

D

D

P

Y

K

N

V

C

I

V

P

P

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S

I
x
V

A

I

Y
|
Y

T

T

D

H

P

P

E

E

V

V

A

A

W

W

V

V

G

G

L

K

T

S

E

E

K

E

E

Q

A

L

K

K

E

E

Q

E

G

G

I

I

A

E

Y

Y

E

K

T

V

A

G

T

K

F

F

P

P

W

F

A

A

S

N

G

S

R

R

A

A

I

K

A

T

S

N

D

A

C

D

S

T

E

D

G

G

M

M

T

V

K

K

L

I

I

L

F

G

D

Q

K

K

D

S

T

T

H

D

R

R

V

V

I

L

G

G

G

A

A

H

I

I

V

L

G

G

V

P

N

G

G

A

G

G

E

E

L

M

L

V

G

N

E

E

I

A

G

A

L

L

A

A

I

L

E

E

M

Y

G

G

C

A

D

S

A

C

E

E

D

D

L

I

A

A

L

R

T

V

I

C

H
|
H

A

A

H
|
H

P

P

T

T

L

L

H

S

E
|
E

S

A

active site: L39 (= L41), C43 (= C45), C48 (= C50), S51 (= S53), V186 (≠ I186), E190 (= E190), H448 (= H444), H450 (= H446), E455 (= E451)
binding flavin-adenine dinucleotide: I10 (≠ L12), G11 (= G13), G13 (= G15), P14 (= P16), G15 (≠ A17), E34 (= E36), K35 (≠ R37), N36 (≠ F38), G41 (= G43), T42 (≠ V44), C43 (= C45), G47 (= G49), C48 (= C50), K52 (= K54), Y116 (≠ F116), G117 (= G117), T146 (≠ A146), G147 (= G147), S166 (= S166), R278 (= R274), F281 (≠ N277), G317 (= G313), D318 (= D314), M324 (= M320), L325 (= L321), A326 (= A322), H327 (= H323)
binding 1,4-dihydronicotinamide adenine dinucleotide: I182 (≠ M182), G183 (= G183), G185 (= G185), V186 (≠ I186), I187 (= I187), E190 (= E190), E206 (= E206), F207 (≠ M207), L208 (≠ F208), I276 (= I272), G277 (= G273), R278 (= R274), M324 (= M320), L325 (= L321), V355 (≠ I351), Y357 (= Y353)

1zmcA Crystal structure of human dihydrolipoamide dehydrogenase complexed to NAD+ (see paper)
43% identity, 94% coverage: 5:452/475 of query aligns to 3:456/472 of 1zmcA

query
sites
1zmcA

I

I

K

D

T

A

Q

D

V

V

V

T

V

V

L
x
I

G
|
G

A

S

G
|
G

P
|
P

A
x
G

G

G

Y

Y

S

V

A

A

F

I

R

K

A

A

D

Q

L

L

G

G

L

F

E

K

T

T

V

V

I

C

V

I

E
|
E

R
x
K

F
x
N

S

E

T

T

L
|
L

G

G

G
|
G

V
x
T

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

H

N

V

N

A

S

K

H

V

Y

I

Y

E

H

E

M

A

A

K

H

A

G

V

T

-

D

-

F

A

A

A

S

H

R

G

G

V

I

V

E

F

M

G

S

E

E

P

V

T

R

I

L

D

N

L

L

D

D

K

K

L

M

R

M

S

E

F

Q

K

K

Q

S

K

T

V

A

I

V

S

K

Q

A

L

L

T

T

G

G

L

I

G

A

G

H

M

L

S

F

K

K

M

Q

R

N

K

K

V

V

N

V

V

H

V

V

N

N

G

G

F
x
Y

G
|
G

K

K

F

I

S

T

G

G

P

K

N

N

S

Q

L

V

E

T

V

A

T

T

A

K

E

A

D

D

G

G

T

G

V

T

T

Q

V

V

V

I

K

D

F

T

D

K

Q

N

A

I

I

L

I

I

A

A

A
x
T

G
|
G

S

S

R

E

P

V

I

T

K

P

L

F

P

P

F

G

I

I

P

T

H

I

E

D

D

E

P

D

R

T

I

I

W

V

D

S

S
|
S

T

T

D

G

A

A

L

L

E

S

L

L

K

K

E

K

V

V

P

P

G

E

K

K

L

M

L

V

V

V

M

I

G
|
G

G

A

G
|
G

I
x
V

I
|
I

G

G

L

V

E
|
E

M

L

G

G

T

S

V

V

Y

W

S

Q

S

R

L

L

G

G

S

A

E

D

I

V

D

T

V

A

V
|
V

E
|
E

M
x
F

F
x
L

D

G

Q

H

V

V

I

G

P

G

A

V

A

G

-

I

D

D

K

M

D

E

V

I

V

S

R

K

V

N

F

F

T

Q

K

R

Q

I

I

L

K

Q

K

K

K

Q

-

G

F

F

N

K

L

F

I

K

L

L

E

N

T

T

K
|
K

V
|
V

T

T

A

G

V

A

E

T

A

K

R

K

E

S

D

D

G

G

-

K

I

I

Y

D

V

V

S

S

M

I

E

E

G

A

K

A

S

S

A

G

-

G

P

K

A

A

E

E

P

V

V

I

R

T

Y

C

D

D

A

V

V

L

L

L

V

V

A

C

I
|
I

G
|
G

R
|
R

T

R

P

P

N
x
F

G

T

K

K

L

N

I

L

D

G

A

L

E

E

K

E

A

L

G

G

V

I

K

E

I

L

D

D

E

P

R
|
R

G

G

F

R

I

I

N

P

V

V

D

N

K

T

Q

R

L

F

R

Q

T

T

N

K

V

I

P

P

H

N

I

I

Y

Y

A

A

I

I

G
|
G

D
|
D

I

V

V

V

G

A

Q

G

P

P

M
|
M

L
|
L

A
|
A

H
|
H

K

K

G

A

V

E

H

D

E

E

G

G

H

I

V

I

A

C

A

V

E

E

V

G

I

M

A

A

G

G

M

G

K

A

H

V

Y

H

F

I

D

D

P

Y

K

N

V

C

I

V

P

P

S

S

I

V

A

I

Y

Y

T

T

D

H

P

P

E

E

V

V

A

A

W

W

V

V

G

G

L

K

T

S

E

E

K

E

E

Q

A

L

K

K

E

E

Q

E

G

G

I

I

A

E

Y

Y

E

K

T

V

A

G

T

K

F

F

P

P

W

F

A

A

S

N

G

S

R

R

A

A

I

K

A

T

S

N

D

A

C

D

S

T

E

D

G

G

M

M

T

V

K

K

L

I

I

L

F

G

D

Q

K

K

D

S

T

T

H

D

R

R

V

V

I

L

G

G

G

A

A

H

I

I

V

L

G

G

V

P

N

G

G

A

G

G

E

E

L

M

L

V

G

N

E

E

I

A

G

A

L

L

A

A

I

L

E

E

M

Y

G

G

C

A

D

S

A

C

E

E

D

D

L

I

A

A

L

R

T

V

I

C

H
|
H

A

A

H
|
H

P

P

T

T

L

L

H

S

E
|
E

S

A

active site: L39 (= L41), C43 (= C45), C48 (= C50), S51 (= S53), V186 (≠ I186), E190 (= E190), H448 (= H444), H450 (= H446), E455 (= E451)
binding flavin-adenine dinucleotide: I10 (≠ L12), G11 (= G13), G13 (= G15), P14 (= P16), G15 (≠ A17), E34 (= E36), K35 (≠ R37), N36 (≠ F38), G41 (= G43), T42 (≠ V44), C43 (= C45), G47 (= G49), C48 (= C50), K52 (= K54), Y116 (≠ F116), G117 (= G117), T146 (≠ A146), G147 (= G147), S166 (= S166), I187 (= I187), F281 (≠ N277), G317 (= G313), D318 (= D314), M324 (= M320), L325 (= L321), A326 (= A322), H327 (= H323)
binding nicotinamide-adenine-dinucleotide: G183 (= G183), G185 (= G185), V205 (= V205), E206 (= E206), F207 (≠ M207), L208 (≠ F208), K240 (= K238), V241 (= V239), I276 (= I272), G277 (= G273), R278 (= R274), R297 (= R293), M324 (= M320)

2eq6A Crystal structure of lipoamide dehydrogenase from thermus thermophilus hb8
44% identity, 95% coverage: 9:460/475 of query aligns to 6:451/460 of 2eq6A

query
sites
2eq6A

V

L

V

I

V

V

L
x
I

G
|
G

A

T

G
|
G

P
|
P

A
x
G

G

G

Y

Y

S

H

A

A

F

I

R

R

A

A

D

Q

L

L

G

G

L

L

E

K

T

V

V

L

I

A

V

V

E
|
E

R
x
A

F

-

S

G

T

E

L
x
V

G

G

G
|
G

V
|
V

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
x
T

K
|
K

A

A

L

L

H

H

V

A

A

A

K

E

V

T

I

L

E

H

A

L

K

K

A

V

V

A

A

E

A

G

H

F

G

G

V

L

V

K

F

-

G

A

E

K

P

P

T

E

I

L

D

D

L

L

D

K

K

K

L

L

R

G

S

G

F

W

K

R

Q

D

K

Q

V

V

I

V

S

K

Q

K

L

L

T

T

G

G

L

V

G

G

G

T

M

L

S

L

K

K

M

G

R

N

K

G

V

V

N

E

V

L

N

R

G

G

F
|
F

G
x
A

K

R

F

L

S

V

G

G

P

P

N

K

S

E

L

V

E

E

V

V

T

G

A

G

E

E

D

R

G

-

T

-

V

-

T

-

V

-

V

Y

K

G

F

A

D

K

Q

S

A

L

I

I

I

L

A
|
A

A
x
T

G
|
G

S

S

R

E

P

P

I

L

K

E

L

L

P

K

F

G

I

F

P

P

H

F

-

G

E

E

D

D

P

-

R

-

I

V

W

W

D

D

S
|
S

T

T

D

R

A

A

L

L

E

K

L

V

K

E

E

E

-

G

V

L

P

P

G

K

K

R

L

L

V

V

M

I

G

G

I
x
A

I

V

G

G

L

L

E
|
E

M

L

G

G

T

Q

V

V

Y

Y

S

R

L

L

G

G

S

A

E

E

I

V

D

T

V

L

V

I

E

E

M

Y

F

M

D

P

Q

E

V

I

I

L

P

P

A

Q

A

G

D

D

K

P

D

E

V

T

V

A

R

A

V

L

F

L

T

R

K

R

Q

A

I

L

K

E

K

K

K

E

-

G

F

I

N

R

L

V

I

R

L

T

E

K

T

T

K

K

V

A

T

V

A

G

V

Y

E

E

A

K

R

K

E

K

D

D

G

G

I

L

Y

H

V

V

S

R

M

L

E

E

-

P

G

A

K

E

S

G

A

G

P

E

A

G

E

E

P

E

V

V

R

V

Y

V

D

D

A

K

V

V

L

L

V

V

A

A

I

V

G

G

R

R

T

K

P

P

N
x
R

G

T

K

E

L

G

I

L

D

G

A

L

E

E

K

K

A

A

G

G

V

V

K

K

I

V

D

D

E

E

R

R

G

G

F

F

I

I

N

R

V

V

D

N

K

A

Q

R

L

M

R

E

T

T

N

S

V

V

P

P

H

G

I

V

Y

Y

A

A

I

I

G

G

D
|
D

I

A

V

A

G

R

Q

P

P

P

M
x
L

L
|
L

A
|
A

H
|
H

K

K

G

A

V

M

H

R

E

E

G

G

H

L

V

I

A

A

E

E

V

N

I

A

A

A

G

G

M

K

K

D

H

S

Y

A

F

F

D

D

P

Y

K

Q

V

V

I

-

P

P

S

S

I

V

A

V

Y
|
Y

T

T

D

S

P

P

E

E

V

W

A

A

W

G

V

V

G

G

L

L

T

T

E

E

K

E

E

E

A

A

K

K

E

R

Q

A

G

G

I

Y

A

K

Y

V

E

K

T

V

A

G

T

K

F

F

P

P

W

L

A

A

S

S

G

G

R

R

A

A

I

L

A

T

S

L

D

G

C

G

S

A

E

E

G

G

M

M

T

V

K

K

L

V

I

V

F

G

D

D

K

E

D

E

T

T

H

D

R

L

V

L

I

L

G

G

G

V

A

F

I

I

V

V

G

G

V

P

N

Q

G

A

G

G

E

E

L

L

L

I

G

A

E

E

I

A

G

A

L

L

A

A

I

L

E

E

M

M

G

G

C

A

D

T

A

L

E

T

D

D

L

L

A

A

L

L

T

T

I

V

H
|
H

A

P

H
|
H

P

P

T

T

L

L

H

S

E
|
E

S

S

V

L

G

M

L

E

A

A

E

E

I

A

Y

F

active site: V37 (≠ L41), C41 (= C45), C46 (= C50), T49 (≠ S53), A176 (≠ I186), E180 (= E190), H435 (= H444), H437 (= H446), E442 (= E451)
binding flavin-adenine dinucleotide: I9 (≠ L12), G10 (= G13), G12 (= G15), P13 (= P16), G14 (≠ A17), E33 (= E36), A34 (≠ R37), G39 (= G43), V40 (= V44), C41 (= C45), G45 (= G49), C46 (= C50), K50 (= K54), F111 (= F116), A112 (≠ G117), A135 (= A145), T136 (≠ A146), G137 (= G147), S155 (= S166), R269 (≠ N277), D306 (= D314), L312 (≠ M320), L313 (= L321), A314 (= A322), H315 (= H323), Y344 (= Y353)

Sites not aligning to the query:

active site: 460

6awaA 1.83 angstrom resolution crystal structure of dihydrolipoyl dehydrogenase from pseudomonas putida in complex with fad and adenosine-5'-monophosphate.
41% identity, 98% coverage: 6:469/475 of query aligns to 4:474/475 of 6awaA

query
sites
6awaA

K

K

T

F

Q

D

V

V

L
x
I

G

G

A

A

G
|
G

P
|
P

A
x
G

G

G

Y

Y

S

V

A

A

F

I

R

K

A

A

D

Q

L

L

G

G

L

L

E

K

T

T

V

A

I

C

V

I

E
|
E

R
x
K

F

Y

S

T

-

D

-

A

-

E

-

G

-

K

-

L

T

A

L
|
L

G

G

V
x
T

C
|
C

L

L

N

N

V

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

H

D

V

S

A

S

K

W

V

K

I

Y

E

K

E

E

A

A

K

K

-

E

A

S

V

F

A

N

A

V

H

H

G

G

V

I

V

S

F

T

G

G

E

E

P

V

T

K

I

M

D

D

L

V

D

A

K

A

L

M

R

V

S

G

F

R

K

K

Q

A

K

G

V

I

I

V

S

K

Q

N

L

L

T

T

G

G

L

V

G

A

G

T

M

L

S

F

K

K

M

A

R

N

K

G

V

V

N

T

V

S

V

I

N

Q

G

G

F
x
H

G
|
G

K

K

F

L

S

L

G

A

P

G

N

K

S

K

L

V

E

E

V

V

T

T

A

K

E

A

D

D

G

G

T

T

V

T

T

E

V

V

V

I

K

E

F

A

D

E

Q

N

A

V

I

I

I

L

A

A

A
x
S

G
|
G

S

S

R

R

P

P

I

I

K

D

L

I

P

P

F

P

I

A

P

P

H

V

E

D

D

Q

P

N

R

V

I

I

W

V

D

D

S

S

T

T

D

G

A

A

L

L

E

E

L

F

K

Q

E

A

V

V

P

P

G

K

K

R

L

L

L

G

V

V

M
x
I

G

G

G

A

G

G

I
x
V

I
|
I

G

G

L

L

E
|
E

M

L

G

G

T

S

V

V

Y

W

S

A

S

R

L

L

G

G

S

A

E

E

I

V

D

T

V

V

V

L

E
|
E

M
x
A

F
x
L

D

D

Q

T

V

F

I

L

P

M

A

A

D

D

K

T

D

A

V

V

V

S

R

K

V

E

F

A

T

Q

K

K

Q

T

I

L

K

T

K

K

K

Q

-

G

F

L

N

D

L

I

I

K

L

L

E

G

T

A

K

R

V
|
V

T

T

A

G

V

S

E

K

A

V

R

N

E

G

D

N

G

E

I

V

-

E

-

V

-

T

Y

Y

V

T

S

N

M

A

E

E

G

G

K

E

S

-

A

-

P

-

A

-

E

Q

P

K

V

I

R

T

Y

F

D

D

A

K

V

L

L

I

V

V

A

A

I
x
V

G

G

R
|
R

T

R

P

P

N

V

G

T

K

T

L

D

I

L

D

L

A

A

E

S

K

D

A

S

G

G

V

V

K

T

I

I

D

D

E

E

R

R

G

G

F

Y

I

I

N

F

V

V

D

D

K

D

Q

Y

L

C

R

A

T

T

N

S

V

V

P

P

H

G

I

V

Y

Y

A

A

I

I

G
|
G

D
|
D

I

V

V

V

G

R

Q

G

P

M

M
|
M

L
|
L

A
|
A

H

H

K

K

G

A

V

S

H

E

E

E

G

G

H

I

V

M

A

V

E

E

V

R

I

I

A

K

G

G

M

H

K

K

H

A

Y

Q

F

M

D

N

P

Y

K

D

V

L

I

I

P

P

S

S

I

V

A

I

Y
|
Y

T

T

D

H

P

P

E

E

V

I

A

A

W

W

V

V

G

G

L

K

T

T

E

E

K

Q

E

A

A

L

K

K

E

A

Q

E

G

G

I

V

A

E

Y

V

E

N

T

V

A

G

T

T

F

F

P

P

W

F

A

A

S

S

G

G

R

R

A

A

I

M

A

A

S

A

D

N

C

D

S

T

E

G

G

G

M

F

T

V

K

K

L

V

I

I

F

A

D

D

K

A

D

K

T

T

H

D

R

R

V

V

I

L

G

G

G

V

A

H

I

V

V

I

G

G

V

P

N

S

G

A

E

E

L

L

L

V

G

Q

E

Q

I

G

G

A

L

I

A

A

I

M

E

E

M

F

G

G

C

T

D

S

A

A

E

E

D

D

L

L

A

G

L

M

T

M

I

V

H
x
F

A

S

H
|
H

P

P

T

T

L

L

H

S

E
|
E

S

A

V

L

G

H

L

E

A

A

E

L

I

A

Y

V

E

N

G

G

S

G

I

A

T

I

D

H

L

V

P

A

N
|
N

active site: L45 (= L41), C49 (= C45), C54 (= C50), S57 (= S53), V191 (≠ I186), E195 (= E190), F449 (≠ H444), H451 (= H446), E456 (= E451), N474 (= N469)
binding adenosine monophosphate: I187 (≠ M182), E211 (= E206), A212 (≠ M207), L213 (≠ F208), V245 (= V239), V277 (≠ I272)
binding flavin-adenine dinucleotide: I10 (≠ L12), G13 (= G15), P14 (= P16), G15 (≠ A17), E34 (= E36), K35 (≠ R37), T48 (≠ V44), C49 (= C45), G53 (= G49), C54 (= C50), K58 (= K54), H121 (≠ F116), G122 (= G117), S151 (≠ A146), G152 (= G147), I192 (= I187), R279 (= R274), G318 (= G313), D319 (= D314), M325 (= M320), L326 (= L321), A327 (= A322), Y358 (= Y353)

Sites not aligning to the query:

active site: 475

P31023 Dihydrolipoyl dehydrogenase, mitochondrial; Dihydrolipoamide dehydrogenase; Glycine cleavage system L protein; Pyruvate dehydrogenase complex E3 subunit; E3; PDC-E3; EC 1.8.1.4 from Pisum sativum (Garden pea) (Lathyrus oleraceus) (see 2 papers)
42% identity, 95% coverage: 6:457/475 of query aligns to 37:491/501 of P31023

query
sites
P31023

K

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I

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x
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x
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x
A

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L

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x
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C

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L

N

N

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C

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67:76 (vs. 36:45, 50% identical) binding
C76 (= C45) modified: Disulfide link with 81, Redox-active
C81 (= C50) modified: Disulfide link with 76, Redox-active
G149 (= G117) binding
D348 (= D314) binding
MLAH 354:357 (= MLAH 320:323) binding

Sites not aligning to the query:

1:31 modified: transit peptide, Mitochondrion

1dxlA Dihydrolipoamide dehydrogenase of glycine decarboxylase from pisum sativum (see paper)
42% identity, 95% coverage: 6:457/475 of query aligns to 3:457/467 of 1dxlA

query
sites
1dxlA

K

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T

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Y

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I

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E

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M

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L

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R

T

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A

active site: L38 (= L41), C42 (= C45), C47 (= C50), S50 (= S53), Y184 (≠ I186), E188 (= E190), H444 (= H444), H446 (= H446), E451 (= E451)
binding flavin-adenine dinucleotide: I9 (≠ L12), P13 (= P16), G14 (≠ A17), E33 (= E36), K34 (≠ R37), R35 (≠ F38), G40 (= G43), T41 (≠ V44), C42 (= C45), G46 (= G49), C47 (= C50), K51 (= K54), Y114 (≠ F116), G115 (= G117), T144 (≠ A146), G145 (= G147), Y184 (≠ I186), I185 (= I187), R274 (= R274), D314 (= D314), M320 (= M320), L321 (= L321), A322 (= A322), H323 (= H323)

Query Sequence

>199618 FitnessBrowser__MR1:199618
MSNEIKTQVVVLGAGPAGYSAAFRAADLGLETVIVERFSTLGGVCLNVGCIPSKALLHVA
KVIEEAKAVAAHGVVFGEPTIDLDKLRSFKQKVISQLTGGLGGMSKMRKVNVVNGFGKFS
GPNSLEVTAEDGTVTVVKFDQAIIAAGSRPIKLPFIPHEDPRIWDSTDALELKEVPGKLL
VMGGGIIGLEMGTVYSSLGSEIDVVEMFDQVIPAADKDVVRVFTKQIKKKFNLILETKVT
AVEAREDGIYVSMEGKSAPAEPVRYDAVLVAIGRTPNGKLIDAEKAGVKIDERGFINVDK
QLRTNVPHIYAIGDIVGQPMLAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTDPEVAW
VGLTEKEAKEQGIAYETATFPWAASGRAIASDCSEGMTKLIFDKDTHRVIGGAIVGVNGG
ELLGEIGLAIEMGCDAEDLALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory