SitesBLAST
Comparing 199996 FitnessBrowser__MR1:199996 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4xckA Vibrio cholerae o395 ribokinase complexed with adp, ribose and cesium ion. (see paper)
51% identity, 99% coverage: 1:301/303 of query aligns to 1:298/306 of 4xckA
- active site: A249 (= A252), A250 (= A253), G251 (= G254), D252 (= D255)
- binding adenosine-5'-diphosphate: T220 (= T223), G222 (= G225), S223 (= S226), V242 (= V245), T247 (= T250), A250 (= A253), F254 (= F257), H276 (≠ N279), A279 (≠ S282), V283 (= V286)
- binding alpha-D-ribofuranose: N11 (= N11), D13 (= D13), G39 (= G39), K40 (= K40), N43 (= N43), A95 (= A98), I107 (= I110), I109 (= I112), E140 (= E143), T248 (≠ V251), D252 (= D255)
1gqtB Activation of ribokinase by monovalent cations (see paper)
49% identity, 98% coverage: 4:301/303 of query aligns to 6:300/307 of 1gqtB
- active site: A251 (= A252), A252 (= A253), G253 (= G254), D254 (= D255)
- binding phosphomethylphosphonic acid adenylate ester: N186 (= N187), T222 (= T223), G224 (= G225), S225 (= S226), A252 (= A253), G253 (= G254), H278 (≠ N279), A281 (≠ S282)
- binding cesium ion: D248 (= D249), I250 (≠ V251), A284 (≠ T285), R287 (= R288), S293 (≠ A294)
- binding alpha-D-ribofuranose: N13 (= N11), D15 (= D13), G41 (= G39), N45 (= N43), E142 (= E143), D254 (= D255)
P0A9J6 Ribokinase; RK; EC 2.7.1.15 from Escherichia coli (strain K12) (see 3 papers)
49% identity, 98% coverage: 4:301/303 of query aligns to 7:301/309 of P0A9J6
1rk2A E. Coli ribokinase complexed with ribose and adp, solved in space group p212121 (see paper)
49% identity, 98% coverage: 4:301/303 of query aligns to 4:298/305 of 1rk2A
- active site: A249 (= A252), A250 (= A253), G251 (= G254), D252 (= D255)
- binding adenosine-5'-diphosphate: T220 (= T223), G222 (= G225), S223 (= S226), A250 (= A253), G251 (= G254), H276 (≠ N279), A279 (≠ S282)
- binding tetrafluoroaluminate ion: G213 (= G216), R215 (≠ T218)
- binding magnesium ion: D246 (= D249), A282 (≠ T285), R285 (= R288), S291 (≠ A294)
- binding alpha-D-ribofuranose: N11 (= N11), D13 (= D13), G38 (= G38), G39 (= G39), K40 (= K40), N43 (= N43), E140 (= E143), D252 (= D255)
8cqxA Ribokinase from t.Sp mutant a92g
40% identity, 98% coverage: 4:301/303 of query aligns to 2:294/300 of 8cqxA
- binding adenosine-5'-diphosphate: N179 (= N187), T217 (= T223), G219 (= G225), A220 (≠ S226), G222 (= G228), F250 (= F257), N272 (= N279), G275 (≠ S282), A276 (= A283), T279 (≠ V286)
- binding magnesium ion: D242 (= D249), T244 (≠ V251), A278 (≠ T285), S287 (≠ A294)
6wk0B Crystal structure of human ribokinase in complex with amppcp and ribose
35% identity, 98% coverage: 4:301/303 of query aligns to 5:304/311 of 6wk0B
- binding phosphomethylphosphonic acid adenylate ester: N186 (= N187), T222 (= T223), G224 (= G225), A225 (≠ S226), G227 (= G228), T243 (≠ A242), V246 (= V245), A254 (= A253), G255 (= G254), N282 (= N279), A285 (≠ S282), A286 (= A283), V289 (= V286)
- binding alpha-D-ribofuranose: D14 (= D13), G40 (= G39), K41 (= K40), N44 (= N43), A96 (= A98), E141 (= E143), D256 (= D255)
5c41A Crystal structure of human ribokinase in complex with amppcp in p21 spacegroup and with 4 protomers
35% identity, 98% coverage: 4:301/303 of query aligns to 5:304/317 of 5c41A
- active site: G253 (≠ A252), A254 (= A253), G255 (= G254), D256 (= D255)
- binding phosphomethylphosphonic acid adenylate ester: N186 (= N187), T222 (= T223), G224 (= G225), A225 (≠ S226), G227 (= G228), V246 (= V245), G255 (= G254), N282 (= N279), A285 (≠ S282), A286 (= A283)
2fv7A Crystal structure of human ribokinase
35% identity, 98% coverage: 4:301/303 of query aligns to 4:303/308 of 2fv7A
- active site: G252 (≠ A252), A253 (= A253), G254 (= G254), D255 (= D255)
- binding adenosine-5'-diphosphate: N185 (= N187), T221 (= T223), G223 (= G225), G226 (= G228), T242 (≠ A242), V245 (= V245), A253 (= A253), G254 (= G254), N281 (= N279), A284 (≠ S282), A285 (= A283), V288 (= V286)
5c3yA Structure of human ribokinase crystallized with amppnp
35% identity, 98% coverage: 4:301/303 of query aligns to 4:303/306 of 5c3yA
- active site: G252 (≠ A252), A253 (= A253), G254 (= G254), D255 (= D255)
- binding amp phosphoramidate: T221 (= T223), G223 (= G225), V245 (= V245), T250 (= T250), G254 (= G254), N281 (= N279), A284 (≠ S282), A285 (= A283)
6wjzA Crystal structure of human ribokinase in complex with ampcp
35% identity, 98% coverage: 4:301/303 of query aligns to 5:304/315 of 6wjzA
- binding phosphomethylphosphonic acid adenosyl ester: N186 (= N187), T222 (= T223), G224 (= G225), A225 (≠ S226), G227 (= G228), T243 (≠ A242), V246 (= V245), A254 (= A253), G255 (= G254), N282 (= N279), A285 (≠ S282), A286 (= A283), V289 (= V286)
6a8cA Ribokinase from leishmania donovani with adp (see paper)
35% identity, 98% coverage: 4:301/303 of query aligns to 16:322/327 of 6a8cA
- binding adenosine-5'-diphosphate: G245 (= G225), A246 (≠ S226), T271 (= T250), A274 (= A253), G275 (= G254), N300 (= N279), A303 (≠ S282)
- binding glycerol: D25 (= D13), S42 (= S30), S44 (= S32), G50 (= G38), G51 (= G39), N55 (= N43)
6a8bA Ribokinase from leishmania donovani with amppcp (see paper)
35% identity, 98% coverage: 4:301/303 of query aligns to 16:322/327 of 6a8bA
- binding phosphomethylphosphonic acid adenylate ester: G245 (= G225), A246 (≠ S226), T271 (= T250), A274 (= A253), G275 (= G254), N300 (= N279), A303 (≠ S282), V307 (= V286)
- binding glycerol: D25 (= D13), G50 (= G38), G51 (= G39), N55 (= N43), N157 (≠ L142), I159 (≠ T144), E190 (≠ A171)
6a8aA Ribokinase from leishmania donovani with atp (see paper)
35% identity, 98% coverage: 4:301/303 of query aligns to 16:322/327 of 6a8aA
- binding adenosine-5'-triphosphate: N207 (= N187), T243 (= T223), G245 (= G225), A246 (≠ S226), G248 (= G228), T271 (= T250), G273 (≠ A252), A274 (= A253), G275 (= G254), N300 (= N279), A303 (≠ S282), V307 (= V286)
- binding glycerol: D25 (= D13), G50 (= G38), G51 (= G39), N55 (= N43)
5byfA Crystal structure of human ribokinase in complex with amp
35% identity, 97% coverage: 8:301/303 of query aligns to 10:305/313 of 5byfA
6znxC Ribokinase from thermus species
38% identity, 98% coverage: 4:301/303 of query aligns to 2:259/265 of 6znxC
Q9H477 Ribokinase; RK; EC 2.7.1.15 from Homo sapiens (Human)
35% identity, 97% coverage: 8:301/303 of query aligns to 22:317/322 of Q9H477
A1A6H3 Ribokinase; AtRBSK; RK; EC 2.7.1.15 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
33% identity, 98% coverage: 4:301/303 of query aligns to 71:371/379 of A1A6H3
Sites not aligning to the query:
- 1:74 mutation Missing: Loss of ribokinase activity.
6ilsB Structure of arabidopsis thaliana ribokinase complexed with ribose and atp (see paper)
33% identity, 99% coverage: 4:303/303 of query aligns to 5:307/313 of 6ilsB
- binding adenosine-5'-triphosphate: N189 (= N187), K225 (≠ T223), G227 (= G225), I246 (= I240), A248 (= A242), A257 (= A253), G258 (= G254), F261 (= F257), A286 (≠ S282), S287 (≠ A283)
- binding alpha-D-ribofuranose: N12 (= N11), D14 (= D13), G40 (= G39), K41 (= K40), N44 (= N43), E144 (= E143), D259 (= D255)
6xk2A Crystal structure of ribokinase from cryptococcus neoformans var. Grubii serotype a in complex with adp
31% identity, 97% coverage: 2:296/303 of query aligns to 4:311/320 of 6xk2A
3ikhA Crystal structure of ribokinase in complex with atp and glycerol in the active site from klebsiella pneumoniae
29% identity, 99% coverage: 3:302/303 of query aligns to 3:282/286 of 3ikhA
Query Sequence
>199996 FitnessBrowser__MR1:199996
MSHILVIGSANADHVMNFEYLPVPGQTLKSRSYRLEHGGKGANQAVACARLCLPDSRVDF
ICHLGQDSIGNEMRDSWLKDGIQAEGITLVDNVSTGTAMIFVADNGENAIGIAAGANAHL
TPLELEKHYALFANAQYLLIQLETPTETVSNALQMAKRLGITTVLNPAPAATQELDYLKW
VDIITPNETEAEALTGIEVKHEEDAKLAAQWLHQQGITTVVITLGSKGAFISSPGFTGLI
PALTVQAIDTVAAGDTFNGALVVGLSEGMSIAAAVSFANAASAITVTREGAQRAIPYRSE
VSR
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory