SitesBLAST

T31 (≠ A10) mutation to I: More active on the (S)-enantiomers of mandelamide and lactamide than the (R)-enantiomers; when associated with N-437.
K100 (= K84) mutation to A: Abolishes activity on mandelamide.
S180 (= S162) mutation to A: Significantly decreases activity on mandelamide.
S181 (= S163) mutation to A: Significantly decreases activity on mandelamide.
G202 (≠ W184) mutation to A: Increase in KM values for aromatic substrates, but not aliphatic substrates. Active against lactamide but not against mandelamide; when associated with H-207 and E-382.; mutation to V: Increase in KM values for aromatic substrates, but not aliphatic substrates.
S204 (= S186) mutation to A: Abolishes activity on mandelamide.
Q207 (≠ G189) mutation to H: Increases activity on lactamide, does not affect activity on mandelamide; when associated with E-382. Active against lactamide but not against mandelamide; when associated with A-202 and E-382. More active on the (S)-enantiomers of mandelamide and lactamide than the (R)-enantiomers; when associated with S-316 and N-437.
S316 (≠ N360) mutation to N: More active on the (S)-enantiomers of mandelamide and lactamide than the (R)-enantiomers; when associated with H-207 and N-437.
Q382 (≠ N428) mutation to H: Increases activity on lactamide, does not affect activity on mandelamide; when associated with H-207. Active against lactamide but not against mandelamide; when associated with A-202 and H-207.
I437 (≠ V500) mutation to N: More active on the (S)-enantiomers of mandelamide and lactamide than the (R)-enantiomers. More active on the (S)-enantiomers of mandelamide and lactamide than the (R)-enantiomers; when associated with I-31. More active on the (S)-enantiomers of mandelamide and lactamide than the (R)-enantiomers; when associated with H-207 and N-316.

6c6gA An unexpected vestigial protein complex reveals the evolutionary origins of an s-triazine catabolic enzyme. Inhibitor bound complex. (see paper)
35% identity, 44% coverage: 11:258/568 of query aligns to 5:247/457 of 6c6gA

query
sites
6c6gA

E

E

L

I

R

I

A

E

A

G

L

I

E

A

S

S

G

G

R

R

T

T

T

S

V

A

V

R

E

D

L

V

V

C

K

E

S

E

Y

A

L

L

A

A

R

T

V

I

A

G

A

A

Y

T

D

D

G

G

P

-

A

-

T

-

E

-

T

-

K

L

L

I

N

N

A

A

L

F

V

T

V

C

H

R

N

T

P

V

D

E

-

R

A

A

L

R

K

A

E

E

A

A

E

D

A

A

S

I

D

D

A

V

R

R

A

A

R

R

G

G

E

E

T

V

L

L

S

P

P

P

L

L

D

A

G

G

I

L

P

P

Y

Y

T

A

A

V

K

K

D

N

S

L

Y

F

L

D

V

I

K

E

G

G

L

V

T

T

A

T

A

L

S

A

G

G

S

S

P

K

A

I

F

N

K

R

D

T

L

L

-

P

V

P

A

A

Q

R

Y

A

D

D

A

A

F

V

A

L

V

V

E

Q

R

R

L

L

R

K

A

A

G

G

A

A

I

V

C

L

L

L

G

G

K

G

T

L

N

N

M

M

P

D

P

E

M

F

A

A

N
x
Y

G

G

-

F

G

T

M

T

Q

E

R

N

G

T

H

H

Y

Y

G

G

R

P

A

T

E

R

S

N

P

P

Y

H

N

D

A

-

E

-

Y

-

L

-

T

T

A

G

P

R

F

I

A

A

S

G

G

G

S

S

N

G

G

G

A

S

G

G

T

A

G

A

T

I

A

A

S

G

Y

Q

S

V

A

P

F

L

G

S

L

L

A

G

E

S

E

D

T

T

W
x
N

S

G

S

S

G

I

R

R

G

V

P

P

A

A

S

S

N

L

N

C

G

G

L

V

C

W

A

G

Y

L

T

K

P

P

S

T

R

F

G

G

V

R

I

L

S

S

V

R

R

R

G

G

N

T

W

Y

P

P

L
x
F

T

V

P

H

T

S

M

I

D

D

V

H

V

L

V

G

P

P

Y

L

T

A

R

D

T

S

M

V

A

E

D

G

M

L

L

A

E

L

V

A

L

Y

D

D

V

A

I

M

V

Q

A

G

D

P

D

D

P

P

I

L

T

D

R

P

G

G

-

C

D

S

L

A

W

S

R

R

L

I

Q

Q

P

P

W

S

V

V

Q

P

I

V

binding calcium ion: Y125 (≠ N134), N172 (≠ W184), F202 (≠ L214)

4gysB Granulibacter bethesdensis allophanate hydrolase co-crystallized with malonate (see paper)
25% identity, 89% coverage: 55:560/568 of query aligns to 46:443/461 of 4gysB

query
sites
4gysB

D

E

A

V

L

L

K

A

E

E

A

A

E

R

A

A

S

L

D

D

A

A

R

S

R

P

A

A

R

T

G

G

E

K

T

-

L

-

S

-

P

P

L

L

D

Y

G

G

I

V

P

P

Y

F

T

A

A

V

K
|
K

D

D

S

N

Y

I

L

D

V

V

K

A

G

G

L

L

T

P

A

C

A

S

S

A

G

A

S

C

P

P

A

A

F

F

K

T

D

-

L

Y

V

E

A

P

Q

D

Y

R

D

D

A

A

F

T

A

V

V

V

E

A

R

R

L

L

R

R

A

A

G

G

A

A

I

I

C

V

L

L

G

G

K

K

T

T

N

N

M

L

P

D

P

Q

M

F

A
|
A

N

T

G
|
G

G

L

M

V

-

G

Q

T

R

R

G

S

H

P

Y

F

G

G

R

A

A

P

E

R

S

C

P

V

Y

F

N

D

A

Q

E

D

Y

Y

L

I

T

S

A

-

P

-

F

-

A

-

S

G

G

G

S
|
S

N

S

G

G

A

S

G

A

T

V

G

A

T

V

A

A

S

G

Y

L

S

V

A

A

F

F

G

S

L

L

A

G

E
x
T

E

D

T
|
T

W
x
A

S
x
G

S
|
S

G

G

R

R

G
x
V

P

P

A

A

S

A

N

F

N

N

G

N

L

L

C

V

A

G

Y

V

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P

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R

K

G

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x
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|
G

N
x
V

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V

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P
x
R

T

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M

L

D

D

V

C

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V

V

T

P

V

Y

F

T

A

R

A

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S

M

V

A

A

D

E

-

G

-

T

-

L

M

I

L

R

E

R

V

I

L

A

D

E

V

G

I

Y

V

D

A

A

D

A

D

D

P

P

I

Y

T

S

R

R

G

P

D

S

L

Q

W

K

R

R

L

R

Q

L

P

P

W

H

V

V

Q

-

I

-

P

-

K

-

A

-

S

-

T

-

V

-

R

-

P

-

E

-

S

-

Y

-

L

-

A

-

L

-

A

-

S

-

A

-

D

-

T

-

L

-

K

-

G

G

K

L

R

R

F

V

G

G

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V

P

P

R

R

M

Q

Y

-

I

-

N

D

K

Q

D

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E

E

L

F

A

Y

G

G

T

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S

T

E

-

N

-

P

-

G

-

I

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G

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P

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T

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G

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Q

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R

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I

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Q

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S

A

V

Y

I

A

D

A

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Y

E

Q

A

R

A

A

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K

D

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M

E

I

A

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A

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G

D

A

A

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E

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L

I

V

E

E

V

I

D

D

F

F

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A

L

P

V

F

S

R

N

D

C

A

E

A

G

K

D

L

R

L

P
x
Y

G

G

A

G

P

P

T

W

V

V

F

A

N

E

R
|
R

G

-

I

-

V

-

T

-

P

-

Q

-

F

-

L

-

H

-

D

-

E

-

L

-

W

-

E

-

L

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S

-

G

-

W

-

A

-

F

-

D

-

E

-

F

-

L

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R

-

A

-

N

-

D

-

P

-

K

-

L

-

N

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K

-

L

L

E

E

D

A

V

V

V

G

G

D

H

H

L

L

I

-

F

-

P

-

H

-

D

-

L

-

C

-

T

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L

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P

-

N

S

R

R

E

A

G

P

D

D

L

-

A

-

G

S

M

F

E

D

E

P

Y

V

V

V

N

R

M

S

A

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E

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T

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A

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K

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T

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A

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D

D

S

A

I

-

P

-

D

-

G

-

L

F

R

R

G

G

L

Q

E

Y

K

E

T

L

R

A

K

A

L

L

D

T

L

Q

E

Q

D

A

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M

A

D

Q

G

W

L

A

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R

L

M

D

D

A

I

V

L

L

L

F

L

P

P

T

T

V

-

A

-

D

-

V

-

G

A

P

P

A

T

D

I

A

H

H

K

V

V

N

E

P

A

A

V

S

M

A

A

D

D

I

P

A

V

W

R

S

L

N

N

G

S

V

Q

W

L

V

G

A

H

N

Y

G

T

N

N

L

F

A

-

I

V

R

N

H

L

L

L

G

D

V

C

P

A

T

A

V

I

T

A

V

V

P

P

M

A

G

G

V

F

M

I

A

-

D

E

I

T

G

G

M

L

P

P

V

F

G

G

L

V

T

T

F

L

A

V

G

G

R

P

A

A

Y

F

D

S

D

D

N

D

N

S

L

M

L

A

K

L

F

I

A

A

G

D

A

R

F

L

E

-

S

-

T

-

G

-

N

H

R

R

I

L

I

E

P

P

active site: K72 (= K84), S146 (= S162), S147 (= S163), T165 (≠ E181), T167 (= T183), A168 (≠ W184), G169 (≠ S185), S170 (= S186), V173 (≠ G189)
binding malonate ion: A120 (= A133), G122 (= G135), S146 (= S162), T167 (= T183), A168 (≠ W184), S170 (= S186), S193 (≠ R209), G194 (= G210), V195 (≠ N211), R200 (≠ P216), Y297 (≠ P353), R305 (= R361)

3h0mA Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
31% identity, 41% coverage: 8:238/568 of query aligns to 6:223/478 of 3h0mA

query
sites
3h0mA

S

S

I

L

A

S

E

E

L

L

R

R

A

E

A

L

L

L

E

K

S

R

G

G

R

E

T

V

T

S

V

P

V

K

E

E

L

V

V

V

K

E

S

S

Y

F

L

Y

A

D

R

R

V

Y

A

N

A

Q

Y

-

D

-

G

-

P

-

A

-

T

T

E

E

T

E

K

K

L

V

N

K

A

A

L

Y

V

I

V

T

-

P

H

L

N

Y

P

G

D

K

A

A

L

L

K

K

E

Q

A

A

E

E

A

S

S

L

D

K

A

E

R

R

R

E

A

L

R

-

G

-

E

-

T

-

L

-

S

-

P

P

L

L

D

F

G

G

I

I

P

P

Y

I

T

A

A

V

K
|
K

D

D

S

N

Y

I

L

L

V

V

K

E

G

G

L

E

T

K

A

T

S

C

G

A

S

S

P

K

A

I

F

L

K

E

D

N

L

F

V

V

A

A

Q

P

Y

Y

D

D

A

A

F

T

A

V

V

I

E

E

R

R

L

L

R

K

A

K

A

A

G

G

A

A

I

L

C

I

L

V

G

G

K

K

T

T

N

N

M

L

P

D

P

E

M

F

A

A

N
x
M

G
|
G

-

S

G

S

M

T

Q

E

R

Y

G

S

H

A

Y

F

G

F

R

P

A

T

E

K

S

N

P

P

Y

W

N

D

A

L

E

E

Y

R

L

V

T

P

A

G

P

G

F
x
S

A
x
S

S

G

G

G

S

S

S

A

N

A

G

S

A

V

G

A

T

V

G

L

T

S

A

A

A

-

S

-

Y

-

S

-

A

P

F

V

G

S

L

L

A

G

E
x
S

E
x
D

T
|
T

W
x
G

S
x
G

S
|
S

G

I

R

R

G
x
Q

P

P

A

A

S

S

N

F

N

C

G

G

L

V

C

I

A

G

Y

I

T

K

P

P

S

T

R

Y

G

G

V

R

I

V

S

S

V

R

R

Y

G

G

N

L

W

V

P

A

L
x
F

T

A

P

S

T

S

M

L

D

D

V

Q

V

I

V

G

P

V

Y

F

T

G

R

R

T

R

M

T

A

E

D

D

M

V

L

A

E

L

V

V

L

L

D

E

V

V

I

I

active site: K72 (= K84), S147 (≠ F158), S148 (≠ A159), S166 (≠ E181), T168 (= T183), G169 (≠ W184), G170 (≠ S185), S171 (= S186), Q174 (≠ G189)
binding glutamine: M122 (≠ N134), G123 (= G135), D167 (≠ E182), T168 (= T183), G169 (≠ W184), G170 (≠ S185), S171 (= S186), F199 (≠ L214)

Sites not aligning to the query:

binding glutamine: 302, 351, 418

3h0lA Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
31% identity, 41% coverage: 8:238/568 of query aligns to 6:223/478 of 3h0lA

query
sites
3h0lA

S

S

I

L

A

S

E

E

L

L

R

R

A

E

A

L

L

L

E

K

S

R

G

G

R

E

T

V

T

S

V

P

V

K

E

E

L

V

V

V

K

E

S

S

Y

F

L

Y

A

D

R

R

V

Y

A

N

A

Q

Y

-

D

-

G

-

P

-

A

-

T

T

E

E

T

E

K

K

L

V

N

K

A

A

L

Y

V

I

V

T

-

P

H

L

N

Y

P

G

D

K

A

A

L

L

K

K

E

Q

A

A

E

E

A

S

S

L

D

K

A

E

R

R

R

E

A

L

R

-

G

-

E

-

T

-

L

-

S

-

P

P

L

L

D

F

G

G

I

I

P

P

Y

I

T

A

A

V

K
|
K

D

D

S

N

Y

I

L

L

V

V

K

E

G

G

L

E

T

K

A

T

S

C

G

A

S

S

P

K

A

I

F

L

K

E

D

N

L

F

V

V

A

A

Q

P

Y

Y

D

D

A

A

F

T

A

V

V

I

E

E

R

R

L

L

R

K

A

K

A

A

G

G

A

A

I

L

C

I

L

V

G

G

K

K

T

T

N

N

M

L

P

D

P

E

M

F

A

A

N

M

G
|
G

-

S

G

S

M

T

Q

E

R

Y

G

S

H

A

Y

F

G

F

R

P

A

T

E

K

S

N

P

P

Y

W

N

D

A

L

E

E

Y

R

L

V

T

P

A

G

P

G

F
x
S

A
x
S

S

G

G

G

S

S

S

A

N

A

G

S

A

V

G

A

T

V

G

L

T

S

A

A

A

-

S

-

Y

-

S

-

A

P

F

V

G

S

L

L

A

G

E
x
S

E

D

T
|
T

W
x
G

S
x
G

S
|
S

G

I

R

R

G
x
Q

P

P

A

A

S

S

N

F

N

C

G

G

L

V

C

I

A

G

Y

I

T

K

P

P

S

T

R

Y

G

G

V

R

I

V

S

S

V

R

R

Y

G

G

N

L

W

V

P

A

L

F

T

A

P

S

T

S

M

L

D

D

V

Q

V

I

V

G

P

V

Y

F

T

G

R

R

T

R

M

T

A

E

D

D

M

V

L

A

E

L

V

V

L

L

D

E

V

V

I

I

active site: K72 (= K84), S147 (≠ F158), S148 (≠ A159), S166 (≠ E181), T168 (= T183), G169 (≠ W184), G170 (≠ S185), S171 (= S186), Q174 (≠ G189)
binding asparagine: G123 (= G135), S147 (≠ F158), G169 (≠ W184), G170 (≠ S185), S171 (= S186)

Sites not aligning to the query:

binding asparagine: 302, 351, 418

5h6sC Crystal structure of hydrazidase s179a mutant complexed with a substrate (see paper)
29% identity, 48% coverage: 3:277/568 of query aligns to 1:274/457 of 5h6sC

query
sites
5h6sC

E

E

V

T

T

T

E

R

V

L

S

T

I

A

A

T

E

E

L

I

R

R

A

A

A

R

L

I

E

S

S

E

G

G

R

A

T

A

T

S

V

R

V

E

E

E

L

V

V

V

K

H

S

E

Y

H

L

L

A

D

R

R

V

I

A

D

A

E

Y

F

D

N

G

-

P

-

A

-

T

-

E

-

T

A

K

L

L

T

N

N

A

S

L

F

V

V

V

E

H

L

N

R

P

A

D

D

-

Q

A

V

L

L

K

E

E

E

A

A

E

R

A

A

S

A

D

D

A

-

R

-

R

R

A

E

R

F

G

G

E

S

T

T

L

L

S

G

-

G

P

P

L

L

D

D

G

G

I

V

P

P

Y

L

T

S

A

I

K
|
K

D

D

S

S

Y

Y

L

S

V

V

K

A

G

G

L

L

T

H

A

R

A

T

S

D

G

G

S

L

P

P

A

V

F

N

K

A

D

D

L

V

V

L

A

D

Q

A

Y

Q

D

D

A

D

F

V

A

A

V

T

E

A

R

R

L

L

R

R

A

A

G

G

A

G

I

L

C

V

L

L

G

G

K

H

T

A

N

G

M

I

P

P

P

D

M

L

A
x
C

-

I

N
x
R

G
x
W

G

N

M

S

Q

V

R

S

G

G

H

L

Y

Y

G

G

R

A

A

V

E

R

S

N

P

P

Y

R

N

D

A

-

E

-

Y

-

L

-

T

L

A

S

P

R

F

T

A

A

S

G

G

G

S
|
S

N

G

G

G

A

D

G

A

T

A

G

N

T

V

A

A

S

G

Y

F

S

A

A

T

F

I

G

G

L

L

A

G

E

G

E

D

T
x
L

W
x
G

S
x
G

S
|
S

G

I

R

R

G

V

P

P

A

A

S

S

N

W

N

C

G

G

L

V

C

Y

A

G

Y

F

T

R

P

T

S

G

R

P

G

G

V

R

I

I

-

P

-

D

-

V

-

N

-

P

-

N

S

G

V

G

R

R

G

S

N

R

W

N

P

V

L

V

T

M

P

E

T

L

M

M

D

A

V

Q

V

I

V

G

P

P

Y

I

T

A

R

R

T

S

M

I

A

D

D

D

M

I

L

E

E

L

V

A

L

F

D

R

V

I

I

M

V

T

A

G

D

V

D

D

-

R

P

D

I

T

T

M

R

S

G

S

D

P

L

L

W

G

R

L

L

I

Q

E

P

P

W

-

V

I

Q

E

I

A

P

P

K

R

A

V

S

A

T

V

V

L

R

R

P

H

E

E

S

T

Y

G

L

A

A

V

L

L

A

D

A

S

S

S

A

V

D

E

active site: K77 (= K84), S152 (= S162), S153 (= S163), L173 (≠ T183), G174 (≠ W184), G175 (≠ S185), S176 (= S186)
binding 4-oxidanylbenzohydrazide: C126 (≠ A133), R128 (≠ N134), W129 (≠ G135), S152 (= S162), L173 (≠ T183), G174 (≠ W184), S176 (= S186)

Sites not aligning to the query:

binding 4-oxidanylbenzohydrazide: 306, 338

Q936X2 Allophanate hydrolase; EC 3.5.1.54 from Pseudomonas sp. (strain ADP) (see paper)
36% identity, 32% coverage: 66:246/568 of query aligns to 74:252/605 of Q936X2

query
sites
Q936X2

R

R

R

K

A

D

R

K

G

G

E

E

T

A

L

L

S

-

P

P

L

L

D

F

G

G

I

I

P

P

Y

F

T

G

A

V

K
|
K

D

D

S

N

Y

I

L

D

V

V

K

A

G

G

L

L

T

P

A

T

S

A

G

G

S

C

P

T

A

G

F

F

K

A

D

R

L

T

V

P

A

R

Q

Q

Y

H

D

-

A

A

F

F

A

V

V

V

E

Q

R

R

L

L

R

V

A

D

A

A

G

G

A

A

I

I

C

P

L

I

G

G

K

K

T

T

N

N

M

L

P

D

P

Q

M

F

A

A

N

T

G

G

-

L

G

N

M

G

Q

T

R

R

G

T

H

P

Y

F

G

G

R

I

A

P

E

R

S

C

P

V

Y

F

N

N

A

E

E

N

Y

Y

L

V

T

S

A

G

P

G

F
x
S

A

S

S

S

G

G

S

S

S

A

N

V

G

A

A

V

G

A

T

N

G

G

T

T

A

V

A

P

S

-

Y

-

S

-

A

-

F

F

G

S

L

L

A

G

E

T

E

D

T

T

W

A

S

G

S
|
S

G

G

R

R

G

I

P

P

A

A

S

A

N

F

N

N

G

N

L

L

C

V

A

G

Y

L

T

K

P

P

S

T

R

K

G

G

V

L

I

F

S

S

V

G

R

S

G

G

N

L

W

V

P

P

L

A

T

A

P

R

T

S

M

L

D

D

V

C

V

I

V

S

P

V

Y

L

T

A

R

H

T

T

M

V

A

D

D

D

M

A

L

L

E

A

V

V

L

A

D

R

V

V

I

A

V

A

-

G

-

Y

-

D

A

A

D

D

P

A

I

F

T

S

R

R

K91 (= K84) mutation to A: Loss of activity.
S165 (≠ F158) mutation to A: Loss of activity.
S189 (= S186) mutation to A: Loss of activity.

3kfuE Crystal structure of the transamidosome (see paper)
28% identity, 41% coverage: 11:244/568 of query aligns to 4:222/468 of 3kfuE

query
sites
3kfuE

E

E

L

I

R

R

A

A

A

R

L

V

E

A

S

R

G

G

R

E

T

V

T

S

V

P

V

L

E

E

L

V

V

A

K

Q

S

A

Y

Y

L

L

A

K

R

R

V

V

A

Q

A

E

Y

L

D

D

G

-

P

-

A

-

T

-

E

-

T

P

K

G

L

L

N

G

A

A

L

F

V

L

V

S

H

L

N

N

P

E

D

R

A

L

L

L

K

E

E

E

A

A

E

E

A

A

S

V

D

D

A

P

R

G

R

-

A

-

R

-

G

-

E

-

T

-

L

-

S

L

P

P

L

L

D

A

G

G

I

L

P

V

Y

V

T

A

A

V

K
|
K

D

D

S

N

Y

I

L

A

V

T

K

R

G

G

L

L

T

R

A

T

S

A

G

G

S

S

P

R

A

L

F

L

K

E

D

N

L

F

V

V

A

P

Q

P

Y

Y

D

E

A

A

F

T

A

A

V

V

E

A

R

R

L

L

R

K

A

A

A

L

G

G

A

A

I

L

C

V

L

L

G

G

K

K

T

T

N

N

M

L

P

D

P

E

M

F

A

G

N

M

G

G

-

S

G

S

M

T

Q

E

R

H

G

S

H

A

Y

F

G

F

R

P

A

T

E

K

S

N

P

P

Y

F

N

D

A

P

E

D

Y

R

L

V

T

P

A

G

P

G

F
x
S

A
x
S

S

G

G

G

S

S

S

A

N

A

G

A

A

L

G

A

T

A

G

D

T

L

A

A

A

P

S

L

Y

A

S

L

A

-

F

-

G

-

L

-

A

G

E
x
S

E

D

T
|
T

W
x
G

S
x
G

S
|
S

G

V

R

R

G
x
Q

P

P

A

A

S

A

N

F

N

C

G

G

L

V

C

Y

A

G

Y

L

T

K

P

P

S

T

R

Y

G

G

V

R

I

V

S

S

V

R

R

F

G

G

N

L

W

I

P

A

L

Y

T

A

P

S

T

S

M

L

D

D

V

Q

V

I

V

G

P

P

Y

M

T

A

R

R

T

S

M

V

A

R

D

D

M

L

L

A

E

L

V

L

L

M

D

D

V

A

I

A

V

A

A

G

D

P

D

D

P

P

I

L

active site: K65 (= K84), S140 (≠ F158), S141 (≠ A159), S159 (≠ E181), T161 (= T183), G162 (≠ W184), G163 (≠ S185), S164 (= S186), Q167 (≠ G189)

Sites not aligning to the query:

binding : 351, 353

2f2aA Structure of tRNA-dependent amidotransferase gatcab complexed with gln (see paper)
24% identity, 51% coverage: 49:337/568 of query aligns to 44:355/485 of 2f2aA

query
sites
2f2aA

L

L

V

A

V

L

H

D

N

K

P

E

D

N

A

A

L

I

K

K

E

K

A

A

E

Q

A

E

S

L

D

D

A

E

R

L

R

Q

A

A

R

K

G

D

E

Q

T

M

L

D

S

G

P

K

L

L

D

F

G

G

I

I

P

P

Y

M

T

G

A

I

K
|
K

D

D

S

N

Y

I

L

I

V

T

K

N

G

G

L

L

T

E

A

T

S

C

G

A

S

S

P

K

A

M

F

L

K

E

D

G

L

F

V

V

A

P

Q

I

Y

Y

D

E

A

S

F

T

A

V

V

M

E

E

R

K

L

L

R

H

A

K

A

E

G

N

A

A

I

V

C

L

L

I

G

G

K

K

T

L

N

N

M

M

P

D

P

E

M

F

A

A

N

M

G
|
G

G

G

-

S

M

T

Q

E

R

T

G

S

H

Y

Y

F

G

K

R

K

A

T

E

V

S

N

P

P

Y

F

N

D

A

H

E

K

Y

A

L

V

T

-

A

-

P

-

F

-

A

P

S

G

G

G

S
|
S

N

G

G

G

A

S

G

A

T

A

G

A

T

V

A

A

S

G

Y

L

S

V

A

P

F

L

G

S

L

L

A

G

E
x
S

E
x
D

T
|
T

W
x
G

S
x
G

S
|
S

G

I

R

R

G
x
Q

P

P

A

A

S

A

N

Y

N

C

G

G

L

V

C

V

A

G

Y

M

T

K

P

P

S

T

R

Y

G

G

V

R

I

V

S

S

V

R

R

F

G

G

N

L

W

V

P

A

L
x
F

T

A

P

S

T

S

M

L

D

D

V

Q

V

I

V

G

P

P

Y

L

T

T

R

R

T

N

M

V

A

K

D

D

M

N

L

A

E

I

V

V

L

L

D

E

V

A

I

I

V

S

A

G

D

A

D

D

-

V

-

N

-

D

-

S

-

T

-

S

-

A

P

P

I

V

T

D

R

D

G

V

D

D

L

-

W

F

R

T

L

S

Q

E

P

I

W

G

V

K

Q

D

I

I

P

K

K

G

A

L

S

K

T

V

V

A

R

L

P

P

E

K

S

E

Y

Y

L

L

-

G

-

E

-

G

-

V

-

A

-

D

-

V

-

K

-

E

A

A

L

V

A

Q

A

N

S

A

A

V

D

E

T

T

L

L

K

K

-

S

-

L

-

G

-

A

-

V

-

E

-

V

-

S

-

L

-

P

G

N

K

T

R

K

F

F

G

G

V

I

P

P

R

S

M
x
Y

Y
|
Y

I

V

N

I

K

A

D

S

E

S

L

E

A

A

G

S

T

S

S

N

E

L

N

S

P

R

G

F

I

D

G

G

G

I

P

R

T

Y

G

G

Q

Y

R

H

I

S

H

K

T

E

R

A

Q

H

S

S

V

L

I

E

D

E

L

L

W

Y

E

K

A

M

A

S

R

R

K

S

A

-

L

-

E

E

A

G

A

F

G

G

A

K

E

E

V

V

active site: K79 (= K84), S154 (= S162), S155 (= S163), S173 (≠ E181), T175 (= T183), G176 (≠ W184), G177 (≠ S185), S178 (= S186), Q181 (≠ G189)
binding glutamine: G130 (= G135), S154 (= S162), D174 (≠ E182), T175 (= T183), G176 (≠ W184), S178 (= S186), F206 (≠ L214), Y309 (≠ M289), Y310 (= Y290)

Sites not aligning to the query:

binding glutamine: 358, 425

2dqnA Structure of tRNA-dependent amidotransferase gatcab complexed with asn (see paper)
24% identity, 51% coverage: 49:337/568 of query aligns to 44:355/485 of 2dqnA

query
sites
2dqnA

L

L

V

A

V

L

H

D

N

K

P

E

D

N

A

A

L

I

K

K

E

K

A

A

E

Q

A

E

S

L

D

D

A

E

R

L

R

Q

A

A

R

K

G

D

E

Q

T

M

L

D

S

G

P

K

L

L

D

F

G

G

I

I

P

P

Y

M

T

G

A

I

K
|
K

D

D

S

N

Y

I

L

I

V

T

K

N

G

G

L

L

T

E

A

T

S

C

G

A

S

S

P

K

A

M

F

L

K

E

D

G

L

F

V

V

A

P

Q

I

Y

Y

D

E

A

S

F

T

A

V

V

M

E

E

R

K

L

L

R

H

A

K

A

E

G

N

A

A

I

V

C

L

L

I

G

G

K

K

T

L

N

N

M

M

P

D

P

E

M

F

A

A

N
x
M

G
|
G

G

G

-

S

M

T

Q

E

R

T

G

S

H

Y

Y

F

G

K

R

K

A

T

E

V

S

N

P

P

Y

F

N

D

A

H

E

K

Y

A

L

V

T

-

A

-

P

-

F

-

A

P

S

G

G

G

S
|
S

N

G

G

G

A

S

G

A

T

A

G

A

T

V

A

A

S

G

Y

L

S

V

A

P

F

L

G

S

L

L

A

G

E
x
S

E

D

T
|
T

W
x
G

S
x
G

S
|
S

G

I

R

R

G
x
Q

P

P

A

A

S

A

N

Y

N

C

G

G

L

V

C

V

A

G

Y

M

T

K

P

P

S

T

R

Y

G

G

V

R

I

V

S

S

V

R

R

F

G

G

N

L

W

V

P

A

L

F

T

A

P

S

T

S

M

L

D

D

V

Q

V

I

V

G

P

P

Y

L

T

T

R

R

T

N

M

V

A

K

D

D

M

N

L

A

E

I

V

V

L

L

D

E

V

A

I

I

V

S

A

G

D

A

D

D

-

V

-

N

-

D

-

S

-

T

-

S

-

A

P

P

I

V

T

D

R

D

G

V

D

D

L

-

W

F

R

T

L

S

Q

E

P

I

W

G

V

K

Q

D

I

I

P

K

K

G

A

L

S

K

T

V

V

A

R

L

P

P

E

K

S

E

Y

Y

L

L

-

G

-

E

-

G

-

V

-

A

-

D

-

V

-

K

-

E

A

A

L

V

A

Q

A

N

S

A

A

V

D

E

T

T

L

L

K

K

-

S

-

L

-

G

-

A

-

V

-

E

-

V

-

S

-

L

-

P

G

N

K

T

R

K

F

F

G

G

V

I

P

P

R

S

M
x
Y

Y
|
Y

I

V

N

I

K

A

D

S

E

S

L

E

A

A

G

S

T

S

S

N

E

L

N

S

P

R

G

F

I

D

G

G

G

I

P

R

T

Y

G

G

Q

Y

R

H

I

S

H

K

T

E

R

A

Q

H

S

S

V

L

I

E

D

E

L

L

W

Y

E

K

A

M

A

S

R

R

K

S

A

-

L

-

E

E

A

G

A

F

G

G

A

K

E

E

V

V

active site: K79 (= K84), S154 (= S162), S155 (= S163), S173 (≠ E181), T175 (= T183), G176 (≠ W184), G177 (≠ S185), S178 (= S186), Q181 (≠ G189)
binding asparagine: M129 (≠ N134), G130 (= G135), T175 (= T183), G176 (≠ W184), S178 (= S186), Y309 (≠ M289), Y310 (= Y290)

Sites not aligning to the query:

binding asparagine: 358, 425

Q7XJJ7 Fatty acid amide hydrolase; AtFAAH; N-acylethanolamine amidohydrolase; EC 3.5.1.99 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
26% identity, 43% coverage: 9:254/568 of query aligns to 131:370/607 of Q7XJJ7

query
sites
Q7XJJ7

I

I

A

R

E

D

L

Y

R

A

A

Y

A

A

L

Y

E

R

S

S

G

K

R

L

T

T

V

P

V

L

E

Q

L

V

V

A

K

K

S

R

Y

I

L

I

A

S

R

I

V

I

A

E

-

E

-

F

A

G

Y

Y

D

D

G

K

P

P

A

P

T

T

E

P

T

F

K

L

L

I

N

R

A

-

L

-

V

-

V

F

H

D

N

A

P

N

D

E

A

V

L

I

K

K

E

Q

A

A

E

E

A

A

S

S

D

T

A

R

R

R

R

F

A

E

R

Q

G

G

E

N

T

P

L

I

S

S

P

V

L

L

D

D

G

G

I

I

P

F

Y

V

T

T

A

I

K
|
K

D

D

S

D

Y

I

-

D

L

C

V

L

K

P

G

H

L

P

T

T

A

N

A

G

S

G

G

T

S

T

P

W

A

L

F

H

K

E

D

D

L

R

V

S

A

V

Q

E

Y

K

D

D

A

S

F

A

A

V

V

V

E

S

R

K

L

L

R

R

A

S

A

C

G

G

A

A

I

I

C

L

L

L

G

G

K

K

T

A

N

N

M

M

P

H

P

E

M

L

A

G

N

M

G

G

G

T

M

T

-

G

Q

N

R

N

G

S

H

N

Y

Y

G

G

R

T

A

T

E

R

S

N

P

P

Y

H

N

D

A

P

E

K

Y

R

L

Y

T

T

A

G

P

-

F

-

A

-

S

-

G

G

S
|
S

N

S

G

G

A

S

G

A

T

A

G

I

T

V

A

A

S

G

Y

L

S

C

A

S

F

A

G

A

L

L

A

G

E

T

E

D

T
x
G

W
x
G

S
x
G

S
|
S

G

V

R
|
R

G

I

P

P

A

S

S

A

N

L

N

C

G

G

L

I

C

T

A

G

Y

L

T

K

P

T

S

T

R

Y

G

G

V

R

I

T

S

D

V

M

R

T

G

G

N

S

W

L

P

C

L

E

T

G

P

G

T

T

M

V

D

E

V

I

V

G

P

P

Y

L

T

A

R

S

T

S

M

L

A

E

D

D

M

A

L

F

E

L

V

V

L

Y

D

A

V

A

I

I

V

L

A

G

D
x
S

D

-

P

-

I

-

T

S

R

S

G

A

D

D

L

R

W

Y

R

N

L

L

Q

K

P

P

K205 (= K84) mutation to A: Loss of activity.
SS 281:282 (= SS 162:163) mutation to AA: Loss of activity.
GGGS 302:305 (≠ TWSS 183:186) binding substrate
S305 (= S186) mutation to A: Loss of activity.
R307 (= R188) mutation to A: Loss of activity.
S360 (≠ D241) mutation to A: No effect.

6diiH Structure of arabidopsis fatty acid amide hydrolase in complex with methyl linolenyl fluorophosphonate (see paper)
26% identity, 43% coverage: 9:254/568 of query aligns to 131:370/616 of 6diiH

query
sites
6diiH

I

I

A

R

E

D

L

Y

R

A

A

Y

A

A

L

Y

E

R

S

S

G

K

R

L

T

T

V

P

V

L

E

Q

L

V

V

A

K

K

S

R