SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 200448 FitnessBrowser__MR1:200448 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 17 hits to proteins with known functional sites (download)

Q9I6J0 Spermidine-binding periplasmic protein SpuE from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
60% identity, 98% coverage: 9:367/367 of query aligns to 8:365/365 of Q9I6J0

query
sites
Q9I6J0

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T35 (≠ S35) binding
E181 (≠ D183) binding
D242 (= D244) binding
N269 (= N271) binding
W271 (= W273) mutation to F: Increases affinity for putrescine (to KD=1.12 uM).

3ttnA Crystal structures of polyamine receptors spud and spue from pseudomonas aeruginosa (see paper)
62% identity, 92% coverage: 29:364/367 of query aligns to 2:335/335 of 3ttnA

query
sites
3ttnA

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binding spermidine: W7 (= W34), T8 (≠ S35), Y10 (= Y37), S55 (= S82), E154 (≠ D183), Y212 (≠ F241), D215 (= D244), W244 (= W273), D246 (= D275)

Q9I6J1 Putrescine-binding periplasmic protein SpuD from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
58% identity, 100% coverage: 1:367/367 of query aligns to 2:367/367 of Q9I6J1

query
sites
Q9I6J1

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SD 36:37 (= SD 35:36) binding
C173 (= C173) modified: Disulfide link with 236
C236 (= C236) modified: Disulfide link with 173
D244 (= D244) binding
F273 (≠ W273) mutation to W: Significant decrease in affinity for putrescine (to KD=0.75 uM).
D275 (= D275) binding

3ttmA Crystal structure of spud in complex with putrescine (see paper)
59% identity, 93% coverage: 27:366/367 of query aligns to 3:341/341 of 3ttmA

query
sites
3ttmA

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F

F

H

H

S

S

R

K

Y

Y

I

I

S

S

D

D

L

L

A

A

N

N

G

G

D

N

I

I

C

C

V

V

A

A

Y

I

G

G

F
x
Y

S

S

G

G

D
|
D

V

I

F

Y

Q

Q

A

A

A

K

A

S

R

R

A

A

D

E

E

E

A

A

G

K

N

N

G

K

N

V

K

T

I

V

G

K

Y

Y

S

N

I

I

P

P

K

K

E

E

G

G

A

A

N

G

L

S

W
x
F

F

F

D
|
D

M

M

L

V

A

A

I

I

P

P

A

K

D

D

A

A

T

E

N

N

V

T

E

E

N

G

A

A

H

L

K

A

L

F

I

V

N

N

Y

F

L

L

L

M

R

K

P

P

E

E

V

I

I

M

A

A

P

E

I

I

S

T

N

D

Y

V

V

V

A

Q

Y
x
F

A

P

N

N

P

G

N

N

D

A

L

A

A

A

Q

T

P

P

L

L

V

V

D

S

E

E

A

A

I

I

R

R

N

N

D

D

P

P

A

G

I

I

Y

Y

P

P

P

S

K

E

E

E

V

V

L

M

D

K

K

K

L

L

Y

Y

I

T

G

F

E

P

I

D

R

L

P

P

L

A

K

K

I

T

Q

Q

R

R

V

A

L

M

T

T

R

R

V

S

W

W

T

T

K

K

V

I

K

K

S

S

G

G

binding 1,4-diaminobutane: W10 (= W34), S11 (= S35), D12 (= D36), Y13 (= Y37), Y216 (≠ F241), D219 (= D244), F248 (≠ W273), D250 (= D275), F286 (≠ Y311)

P31133 Putrescine-binding periplasmic protein PotF from Escherichia coli (strain K12) (see 3 papers)
55% identity, 98% coverage: 10:367/367 of query aligns to 17:370/370 of P31133

query
sites
P31133

L
|
L

A
x
M

L
x
A

V
|
V

T
x
S

A
x
V

G
|
G

T
|
T

L
|
L

A
|
A

S

A

V

-

A

-

V

-

Q

-

A

E

E

Q

E

K

V

T

V

L

R

H

M

I

Y

Y

N

N

W

W

S
|
S

D

D

Y

Y

I

I

A

A

E

P

D

D

T

T

L

V

E

A

N

N

F

F

K

E

K

K

E

E

T

T

G

G

I

I

R

K

V

V

I

V

Y

Y

D

D

V

V

F

F

D

D

S

S

N

N

E

E

V

V

V

L

E

E

A

G

K

K

L

L

L

M

S

A

G

G

R

S

S

T

G

G

Y

F

D

D

I

L

V

V

P

P

S

S

N

A

H

S

F

F

L

L

A

E

K

R

Q

Q

I

L

K

T

A

A

G

G

A

V

F

F

K

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

T

P

N

E

F

W

K

K

N

N

L

L

N

D

A

P

D

E

L

L

M

L

K

K

L

L

L

V

E

A

K

K

A

H

D

D

P

P

G

D

N

N

Q

K

Y

F

A

A

V

M

P

P

Y

Y

L

M

W

W

G

A

T

T

N

T

G

G

I

I

G

G

Y

Y

N

N

I

V

N

D

K

K

V

V

K

K

A

A

A

V

V

L

G

G

E

E

D

N

A

A

P

P

P

-

F

V

N

D

S

S

M

W

E

D

L

L

I

I

F

L

N

K

P

P

K

E

Y

N

A

L

E

E

K

K

I

L

S

K

K

S

C
|
C

G

G

F

V

A

S

M

F

L

L

D

D

S

A

A

P

D

E

M

V

V

F

P

A

Q

T

A

V

M

L

I

N

Y

Y

L

L

G

G

L

K

D

D

P

P

N

N

S

S

T

T

K

K

A

A

E

D

D

D

Y

Y

E

T

-

G

K

P

A

A

G

T

E

D

L

L

E

L

K

K

I

L

R

R

P

P

Y

N

V

I

T

R

Y

Y

F

F

H

H

S

S

R

Q

Y

Y

I

I

S

N

D

D

L

L

A

A

N

N

G

G

D

D

I

I

C
|
C

V

V

A

A

Y

I

G

G

F

W

S

A

G

G

D
|
D

V

V

F

W

Q

Q

A

A

A

S

A

N

R

R

A

A

D

K

E

E

A

A

G

K

N

N

G

G

N

V

K

N

I

V

G

S

Y

F

S

S

I

I

P

P

K

K

E

E

G

G

A

A

N

M

L

A

W

F

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

A

A

D

D

A

A

T

K

N

N

V

K

E

D

N

E

A

A

H

Y

K

Q

L

F

I

L

N

N

Y

Y

L

L

R

R

P

P

E

D

V

V

I

V

A

A

P

H

I

I

S

S

N

D

Y

H

V

V

A

F

Y

Y

A

A

N

N

P

A

N

N

D

K

L

A

A

A

Q

T

P

P

L

L

V

V

D

S

E

A

A

E

I

V

R

R

N

E

D

N

P

P

A

G

I

I

Y

Y

P

P

K

A

E

D

V

V

L

R

D

A

K

K

L

L

Y

F

I

T

G

L

E

K

I

V

R

Q

P

D

L

P

K

K

I

I

Q

D

R

R

V

V

L

R

T

T

R

R

V

A

W

W

T

T

K

K

V

V

K

K

S

S

G

G

Q

K

S38 (= S35) binding
C175 (= C173) modified: Disulfide link with 239
C239 (= C236) modified: Disulfide link with 175
D247 (= D244) binding
D278 (= D275) binding

Sites not aligning to the query:

1:26 signal peptide

7oywA E.Coli's putrescine receptor variant potf/d (4jdf) with mutations e39d f88l s247d in complex with spermidine (see paper)
55% identity, 93% coverage: 26:367/367 of query aligns to 1:342/348 of 7oywA

query
sites
7oywA

E

Q

E

K

V

T

V

L

R

H

M

I

Y

Y

N

N

W
|
W

S
x
T

D

D

Y
|
Y

I

I

A

A

E

P

D

D

T

T

L

V

E

A

N

N

F

F

K

E

K

K

E

E

T

T

G

G

I

I

R

K

V

V

I

V

Y

Y

D

D

V

V

F

F

D

D

S

S

N

N

E

E

V

V

V

L

E

E

A

G

K

K

L

L

L

M

S

A

G

G

R

S

S

T

G

G

Y

F

D

D

I

L

V

V

P

P

S
|
S

N

A

H

Y

F

L

L

L

A

E

K

R

Q

Q

I

L

K

T

A

A

G

G

A

V

F

F

K

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

T

P

N

E

F

W

K

K

N

N

L

L

N

D

A

P

D

E

L

L

M

L

K

K

L

L

L

V

E

A

K

K

A

H

D

D

P

P

G

D

N

N

Q

K

Y

F

A

A

V

M

P

P

Y

Y

L

M

W

W

G

A

T

T

N

T

G

G

I

I

G

G

Y

Y

N

N

I

V

N

D

K

K

V

V

K

K

A

A

A

V

V

L

G

G

E

E

D

N

A

A

P

P

P

-

F

V

N

D

S

S

M

W

E

D

L

L

I

I

F

L

N

K

P

P

K

E

Y

N

A

L

E

E

K

K

I

L

S

K

K

S

C

C

G

G

F

V

A

S

M

F

L

L

D

D

S

D

A

P

D

E

D
x
E

M

V

V

F

P

A

Q

T

A

V

M

L

I

N

Y

Y

L

L

G

G

L

K

D

D

P

P

N

N

S

S

T

T

K

K

A

A

E

D

D

D

Y

Y

E

T

-

G

K

P

A

A

G

T

E

D

L

L

E

L

K

K

I

L

R

R

P

P

Y

N

V

I

T

R

Y

Y

F

F

H

H

S

S

R

Q

Y

Y

I

I

S

N

D

D

L

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

Y

I

G

G

F
x
W

S

A

G

G

D
|
D

V

V

F

W

Q

Q

A

A

A

S

A

N

R

R

A

A

D

K

E

E

A

A

G

K

N

N

G

G

N

V

K

N

I

V

G

S

Y

F

S

S

I

I

P

P

K

K

E

E

G

G

A

A

N

M

L

A

W
|
W

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

A

A

D

D

A

A

T

K

N

N

V

K

E

D

N

E

A

A

H

Y

K

Q

L

F

I

L

N

N

Y

Y

L

L

R

R

P

P

E

D

V

V

I

V

A

A

P

H

I

I

S

S

N

D

Y

H

V

V

A

F

Y
|
Y

A

A

N

N

P

A

N

N

D

K

L

A

A

A

Q

T

P

P

L

L

V

V

D

S

E

A

A

E

I

V

R

R

N

E

D

N

P

P

A

G

I

I

Y

Y

P

P

P
|
P

K
x
A

E

D

V

V

L
x
R

D

A

K

K

L

L

Y

F

I

T

G

Q

E

K

I

V

R

Q

P

D

L

P

K

K

I

I

Q

D

R

R

V

V

L

R

T

T

R

R

V

A

W

W

T

T

K

K

V

V

K

K

S

S

G

G

Q

K

binding (2~{S})-1-(2-methoxyethoxy)propan-2-amine: P310 (= P335), A311 (≠ K336), R314 (≠ L339)
binding spermidine: W9 (= W34), T10 (≠ S35), Y12 (= Y37), S57 (= S82), E157 (≠ D183), W216 (≠ F241), D219 (= D244), W248 (= W273), D250 (= D275), Y286 (= Y311)

7oyxB E.Coli's putrescine receptor variant potf/d (4jdf) with mutations e39d y87s f88y s247d in complex with spermidine (see paper)
55% identity, 93% coverage: 26:367/367 of query aligns to 1:342/347 of 7oyxB

query
sites
7oyxB

E

Q

E
x
K

V
x
T

V

L

R

H

M

I

Y

Y

N

N

W
|
W

S
x
T

D
|
D

Y
|
Y

I

I

A

A

E

P

D

D

T

T

L

V

E

A

N

N

F

F

K

E

K

K

E

E

T

T

G

G

I

I

R

K

V

V

I

V

Y

Y

D

D

V
|
V

F

F

D

D

S

S

N

N

E

E

V

V

V

L

E

E

A

G

K

K

L

L

L

M

S

A

G

G

R

S

S

T

G

G

Y

F

D

D

I

L

V

V

P

P

S
|
S

N

A

H

S

F

Y

L

L

A

E

K

R

Q

Q

I

L

K

T

A
|
A

G
|
G

A

V

F

F

K

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

T

P

N

E

F

W

K

K

N

N

L

L

N

D

A

P

D

E

L

L

M

L

K

K

L

L

L

V

E

A

K
|
K

A

H

D

D

P

P

G

D

N

N

Q

K

Y

F

A

A

V

M

P

P

Y

Y

L

M

W
|
W

G

A

T

T

N

T

G

G

I

I

G

G

Y

Y

N

N

I

V

N

D

K

K

V

V

K

K

A

A

A

V

V

L

G

G

E

E

D

N

A

A

P

P

P

-

F

V

N

D

S

S

M

W

E

D

L

L

I

I

F

L

N

K

P

P

K

E

Y

N

A

L

E

E

K

K

I

L

S

K

K

S

C

C

G

G

F

V

A

S

M

F

L

L

D

D

S

D

A

P

D

E

D
x
E

M

V

V

F

P

A

Q

T

A

V

M

L

I

N

Y

Y

L

L

G

G

L

K

D

D

P

P

N

N

S

S

T

T

K

K

A

A

E

D

D

D

Y

Y

E

T

-

G

K

P

A

A

G

T

E

D

L

L

E

L

K

K

I

L

R

R

P

P

Y

N

V

I

T

R

Y

Y

F

F

H

H

S

S

R

Q

Y

Y

I

I

S

N

D

D

L

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

Y

I

G

G

F
x
W

S

A

G

G

D
|
D

V

V

F

W

Q

Q

A

A

A

S

A

N

R

R

A

A

D

K

E

E

A

A

G

K

N

N

G

G

N

V

K

N

I

V

G

S

Y

F

S

S

I

I

P

P

K

K

E

E

G

G

A

A

N

M

L

A

W
|
W

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

A

A

D

D

A

A

T
x
K

N
|
N

V

K

E

D

N

E

A

A

H

Y

K

Q

L

F

I

L

N

N

Y

Y

L

L

R

R

P

P

E

D

V

V

I

V

A

A

P

H

I

I

S

S

N

D

Y

H

V

V

A

F

Y
|
Y

A

A

N

N

P

A

N

N

D

K

L

A

A

A

Q

T

P

P

L

L

V

V

D

S

E
x
A

A
x
E

I

V

R

R

N

E

D

N

P

P

A

G

I

I

Y

Y

P

P

K

A

E

D

V

V

L
x
R

D

A

K

K

L

L

Y

F

I

T

G

Q

E

K

I
x
V

R

Q

P

D

L

P

K

K

I

I

Q

D

R

R

V

V

L

R

T

T

R

R

V

A

W

W

T

T

K

K

V

V

K

K

S

S

G

G

Q

K

binding (2~{S})-1-methoxypropan-2-amine: K2 (≠ E27), T3 (≠ V28), T10 (≠ S35), D11 (= D36), V33 (= V58), W107 (= W132), K259 (≠ T284), N260 (= N285), R314 (≠ L339)
binding (2~{R})-1-[(2~{R})-1-[(2~{S})-1-[(2~{S})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-yl]oxypropan-2-yl]oxypropan-2-amine: K94 (= K119), V322 (≠ I347)
binding (2~{R})-1-(2-methoxyethoxy)propan-2-amine: A299 (≠ E324), E300 (≠ A325)
binding (2~{S})-1-(2-methoxyethoxy)propan-2-amine: A67 (= A92), G68 (= G93)
binding spermidine: W9 (= W34), T10 (≠ S35), Y12 (= Y37), S57 (= S82), E157 (≠ D183), W216 (≠ F241), D219 (= D244), W248 (= W273), D250 (= D275), Y286 (= Y311)

7oyvA E.Coli's putrescine receptor variant potf/d (4jdf) with mutations e39d f88a s247d in complex with spermidine (see paper)
56% identity, 93% coverage: 26:366/367 of query aligns to 1:341/341 of 7oyvA

query
sites
7oyvA

E
x
Q

E
x
K

V
x
T

V

L

R

H

M

I

Y

Y

N

N

W
|
W

S
x
T

D

D

Y
|
Y

I

I

A

A

E

P

D

D

T

T

L

V

E

A

N

N

F

F

K
x
E

K
|
K

E

E

T

T

G

G

I

I

R
x
K

V

V

I

V

Y

Y

D

D

V

V

F

F

D

D

S

S

N

N

E

E

V

V

V

L

E

E

A

G

K

K

L

L

L

M

S

A

G

G

R

S

S

T

G

G

Y

F

D

D

I

L

V

V

P

P

S
|
S

N

A

H

Y

F

A

L

L

A

E

K

R

Q

Q

I

L

K

T

A

A

G

G

A

V

F

F

K

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

T

P

N

E

F

W

K

K

N

N

L

L

N

D

A

P

D

E

L

L

M

L

K

K

L

L

L

V

E

A

K

K

A

H

D

D

P

P

G

D

N

N

Q

K

Y

F

A

A

V

M

P

P

Y

Y

L

M

W

W

G

A

T

T

N

T

G

G

I

I

G

G

Y

Y

N

N

I

V

N

D

K

K

V

V

K

K

A

A

A

V

V

L

G

G

E

E

D

N

A

A

P

P

P

-

F

V

N

D

S

S

M

W

E

D

L

L

I

I

F

L

N

K

P

P

K

E

Y

N

A

L

E

E

K

K

I

L

S

K

K

S

C

C

G

G

F

V

A

S

M

F

L

L

D

D

S

D

A

P

D

E

D
x
E

M

V

V

F

P

A

Q

T

A

V

M

L

I

N

Y

Y

L

L

G

G

L

K

D

D

P

P

N

N

S

S

T

T

K

K

A

A

E

D

D

D

Y

Y

E

T

-

G

K

P

A

A

G

T

E

D

L

L

E

L

K

K

I

L

R

R

P

P

Y

N

V

I

T

R

Y

Y

F

F

H

H

S

S

R

Q

Y

Y

I

I

S

N

D

D

L

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

Y

I

G

G

F
x
W

S

A

G

G

D
|
D

V

V

F

W

Q

Q

A

A

A

S

A

N

R

R

A

A

D

K

E

E

A

A

G

K

N

N

G

G

N

V

K

N

I

V

G

S

Y

F

S

S

I

I

P

P

K

K

E

E

G

G

A

A

N

M

L

A

W
|
W

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

A

A

D

D

A

A

T

K

N
|
N

V

K

E

D

N

E

A

A

H

Y

K

Q

L

F

I

L

N

N

Y

Y

L

L

R

R

P

P

E

D

V

V

I

V

A

A

P

H

I

I

S

S

N

D

Y

H

V

V

A

F

Y
|
Y

A

A

N

N

P

A

N

N

D

K

L

A

A

A

Q

T

P

P

L

L

V

V

D

S

E

A

A

E

I

V

R

R

N

E

D

N

P

P

A

G

I

I

Y

Y

P

P

P
|
P

K
x
A

E

D

V

V

L
x
R

D

A

K

K

L

L

Y

F

I

T

G

Q

E

K

I

V

R

Q

P

D

L

P

K

K

I

I

Q

D

R

R

V

V

L

R

T

T

R

R

V

A

W

W

T

T

K

K

V

V

K

K

S

S

G

G

binding (2~{S})-1-methoxypropan-2-amine: K2 (≠ E27), T3 (≠ V28), N260 (= N285)
binding (2~{R})-1-[(2~{R})-1-[(2~{R})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-yl]oxypropan-2-amine: Q1 (≠ E26), E22 (≠ K47), K23 (= K48), K28 (≠ R53)
binding (2R)-1-methoxypropan-2-amine: P310 (= P335), A311 (≠ K336), R314 (≠ L339)
binding spermidine: W9 (= W34), T10 (≠ S35), Y12 (= Y37), S57 (= S82), E157 (≠ D183), W216 (≠ F241), D219 (= D244), W248 (= W273), D250 (= D275), Y286 (= Y311)

6ye7A E.Coli's putrescine receptor potf complexed with cadaverine (see paper)
56% identity, 93% coverage: 26:366/367 of query aligns to 1:341/341 of 6ye7A

query
sites
6ye7A

E

Q

E

K

V

T

V

L

R

H

M

I

Y

Y

N

N

W
|
W

S
|
S

D

D

Y
|
Y

I

I

A

A

E

P

D

D

T

T

L

V

E

A

N

N

F

F

K

E

K

K

E

E

T

T

G

G

I

I

R

K

V

V

I

V

Y

Y

D

D

V

V

F

F

D

D

S

S

N

N

E

E

V

V

V

L

E

E

A

G

K

K

L

L

L

M

S

A

G

G

R

S

S

T

G

G

Y

F

D

D

I

L

V

V

P

P

S

S

N

A

H

S

F

F

L

L

A

E

K

R

Q

Q

I

L

K

T

A

A

G

G

A

V

F

F

K

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

T

P

N

E

F

W

K

K

N

N

L

L

N

D

A

P

D

E

L

L

M

L

K

K

L

L

L

V

E

A

K

K

A

H

D

D

P

P

G

D

N

N

Q

K

Y

F

A

A

V

M

P

P

Y

Y

L

M

W

W

G

A

T

T

N

T

G

G

I

I

G

G

Y

Y

N

N

I

V

N

D

K

K

V

V

K

K

A

A

A

V

V

L

G

G

E

E

D

N

A

A

P

P

P

-

F

V

N

D

S

S

M

W

E

D

L

L

I

I

F

L

N

K

P

P

K

E

Y

N

A

L

E

E

K

K

I

L

S

K

K

S

C

C

G

G

F

V

A

S

M

F

L

L

D

D

S

A

A

P

D

E

M

V

V

F

P

A

Q

T

A

V

M

L

I

N

Y

Y

L

L

G

G

L

K

D

D

P

P

N

N

S

S

T

T

K

K

A

A

E

D

D

D

Y

Y

E

T

-

G

K

P

A

A

G

T

E

D

L

L

E

L

K

K

I

L

R

R

P

P

Y

N

V

I

T

R

Y

Y

F

F

H

H

S

S

R

Q

Y

Y

I

I

S

N

D

D

L

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

Y

I

G

G

F
x
W

S

A

G

G

D
|
D

V

V

F

W

Q

Q

A

A

A

S

A

N

R

R

A

A

D

K

E

E

A

A

G

K

N

N

G

G

N

V

K

N

I

V

G

S

Y

F

S

S

I

I

P

P

K

K

E

E

G

G

A

A

N

M

L

A

W
x
F

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

A

A

D

D

A

A

T

K

N

N

V

K

E

D

N

E

A

A

H

Y

K

Q

L

F

I

L

N

N

Y

Y

L

L

R

R

P

P

E

D

V

V

I

V

A

A

P

H

I

I

S

S

N

D

Y

H

V

V

A

F

Y
|
Y

A

A

N

N

P

A

N

N

D

K

L

A

A

A

Q

T

P

P

L

L

V

V

D

S

E

A

A

E

I

V

R

R

N

E

D

N

P

P

A

G

I

I

Y

Y

P

P

K

A

E

D

V

V

L

R

D

A

K

K

L

L

Y

F

I

T

G

L

E

K

I

V

R

Q

P

D

L

P

K

K

I

I

Q

D

R

R

V

V

L

R

T

T

R

R

V

A

W

W

T

T

K

K

V

V

K

K

S

S

G

G

binding pentane-1,5-diamine: W9 (= W34), S10 (= S35), Y12 (= Y37), W216 (≠ F241), D219 (= D244), F248 (≠ W273), D250 (= D275), Y286 (= Y311)

6ye6A E.Coli's putrescine receptor potf complexed with agmatine (see paper)
56% identity, 93% coverage: 26:366/367 of query aligns to 1:341/341 of 6ye6A

query
sites
6ye6A

E

Q

E

K

V

T

V

L

R

H

M

I

Y

Y

N

N

W
|
W

S
|
S

D

D

Y
|
Y

I

I

A

A

E

P

D

D

T

T

L

V

E

A

N

N

F

F

K
x
E

K

K

E

E

T
|
T

G

G

I

I

R
x
K

V

V

I

V

Y

Y

D

D

V

V

F

F

D

D

S

S

N

N

E

E

V

V

V

L

E

E

A

G

K

K

L

L

L

M

S

A

G

G

R

S

S

T

G

G

Y

F

D

D

I

L

V

V

P

P

S

S

N

A

H

S

F

F

L

L

A

E

K

R

Q

Q

I

L

K

T

A

A

G

G

A

V

F

F

K

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

T

P

N

E

F

W

K

K

N

N

L

L

N
x
D

A

P

D

E

L

L

M

L

K

K

L
|
L

L

V

E

A

K

K

A

H

D

D

P

P

G

D

N

N

Q

K

Y

F

A

A

V

M

P

P

Y

Y

L

M

W
|
W

G

A

T

T

N

T

G

G

I

I

G

G

Y

Y

N

N

I

V

N

D

K

K

V

V

K

K

A

A

A

V

V

L

G

G

E

E

D

N

A

A

P

P

P

-

F

V

N

D

S

S

M

W

E

D

L

L

I

I

F

L

N

K

P

P

K

E

Y

N

A

L

E

E

K

K

I

L

S

K

K

S

C

C

G

G

F

V

A

S

M

F

L

L

D

D

S

A

A

P

D

E

D
x
E

M

V

V

F

P

A

Q

T

A

V

M

L

I

N

Y

Y

L

L

G

G

L

K

D

D

P

P

N

N

S

S

T

T

K
|
K

A
|
A

E
x
D

D

D

Y

Y

E

T

-

G

K

P

A

A

G

T

E

D

L

L

E

L

K

K

I

L

R

R

P

P

Y

N

V

I

T

R

Y

Y

F

F

H

H

S

S

R

Q

Y

Y

I

I

S

N

D

D

L

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

Y

I

G

G

F
x
W

S

A

G

G

D
|
D

V

V

F

W

Q

Q

A

A

A

S

A

N

R

R

A

A

D

K

E

E

A

A

G

K

N

N

G

G

N

V

K

N

I

V

G

S

Y
x
F

S
|
S

I
|
I

P

P

K

K

E

E

G

G

A

A

N

M

L

A

W
x
F

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

A

A

D

D

A

A

T

K

N

N

V

K

E

D

N

E

A

A

H

Y

K
x
Q

L

F

I

L

N

N

Y

Y

L

L

R
|
R

P

P

E

D

V

V

I

V

A

A

P

H

I

I

S

S

N

D

Y

H

V

V

A

F

Y
|
Y

A

A

N
|
N

P

A

N

N

D
x
K

L

A

A

A

Q

T

P

P

L

L

V

V

D

S

E

A

A

E

I

V

R

R

N

E

D

N

P

P

A

G

I

I

Y
|
Y

P

P

K

A

E

D

V

V

L

R

D

A

K

K

L

L

Y

F

I

T

G

L

E

K

I

V

R

Q

P

D

L

P

K
|
K

I

I

Q

D

R

R

V

V

L

R

T

T

R

R

V

A

W

W

T

T

K

K

V

V

K

K

S

S

G

G

binding agmatine: W9 (= W34), S10 (= S35), Y12 (= Y37), E157 (≠ D183), W216 (≠ F241), D219 (= D244), F248 (≠ W273), D250 (= D275), Y286 (= Y311)
binding (2R)-1-methoxypropan-2-amine: T25 (= T50), D85 (≠ N110), W107 (= W132), K174 (= K200), A175 (= A201), D176 (≠ E202), F238 (≠ Y263), S239 (= S264), I240 (= I265), Q266 (≠ K291), R273 (= R298), N288 (= N313), K291 (≠ D316), Y308 (= Y333), K326 (= K351)
binding (2~{S})-1-(2-methoxyethoxy)propan-2-amine: L91 (= L116), W107 (= W132)
binding (2~{R})-1-[(2~{R})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-amine: E22 (≠ K47), K28 (≠ R53)

6ye0B E.Coli's putrescine receptor potf complexed with putrescine (see paper)
56% identity, 93% coverage: 26:366/367 of query aligns to 1:341/341 of 6ye0B

query
sites
6ye0B

E

Q

E

K

V

T

V

L

R

H

M

I

Y

Y

N

N

W
|
W

S
|
S

D

D

Y
|
Y

I

I

A

A

E

P

D

D

T

T

L

V

E

A

N

N

F

F

K

E

K

K

E

E

T

T

G

G

I

I

R

K

V

V

I

V

Y

Y

D

D

V

V

F
|
F

D
|
D

S

S

N

N

E

E

V

V

V

L

E

E

A

G

K

K

L

L

L

M

S

A

G

G

R

S

S

T

G

G

Y

F

D

D

I

L

V

V

P

P

S

S

N

A

H

S

F

F

L

L

A

E

K

R

Q

Q

I

L

K

T

A

A

G

G

A

V

F

F

K

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

T

P

N

E

F

W

K

K

N

N

L

L

N

D

A

P

D

E

L

L

M

L

K

K

L
|
L

L

V

E

A

K

K

A

H

D

D

P

P

G

D

N

N

Q

K

Y

F

A

A

V

M

P

P

Y

Y

L

M

W

W

G

A

T

T

N

T

G

G

I

I

G

G

Y

Y

N

N

I

V

N

D

K

K

V

V

K

K

A

A

A

V

V

L

G

G

E

E

D

N

A

A

P

P

P

-

F

V

N

D

S

S

M

W

E

D

L

L

I

I

F

L

N

K

P

P

K

E

Y

N

A

L

E

E

K

K

I

L

S

K

K

S

C

C

G

G

F

V

A

S

M

F

L

L

D

D

S

A

A

P

D

E

M

V

V

F

P

A

Q

T

A

V

M

L

I

N

Y

Y

L

L

G

G

L

K

D

D

P

P

N

N

S

S

T

T

K

K

A

A

E

D

D

D

Y

Y

E

T

-

G

K

P

A

A

G

T

E

D

L

L

E

L

K

K

I

L

R

R

P

P

Y

N

V

I

T

R

Y

Y

F

F

H

H

S

S

R

Q

Y

Y

I

I

S

N

D

D

L

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

Y

I

G

G

F
x
W

S

A

G

G

D
|
D

V

V

F

W

Q

Q

A

A

A

S

A

N

R

R

A

A

D

K

E

E

A

A

G

K

N

N

G

G

N

V

K

N

I

V

G

S

Y

F

S

S

I

I

P

P

K

K

E

E

G

G

A

A

N

M

L

A

W
x
F

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

A

A

D

D

A

A

T

K

N

N

V

K

E

D

N

E

A

A

H

Y

K

Q

L

F

I

L

N

N

Y

Y

L

L

R

R

P

P

E

D

V

V

I

V

A

A

P

H

I

I

S

S

N

D

Y

H

V

V

A

F

Y
|
Y

A

A

N

N

P

A

N

N

D

K

L

A

A

A

Q

T

P

P

L

L

V

V

D

S

E

A

A

E

I

V

R

R

N

E

D

N

P

P

A

G

I

I

Y

Y

P

P

K

A

E

D

V

V

L
x
R

D

A

K

K

L

L

Y

F

I

T

G

L

E

K

I

V

R

Q

P

D

L

P

K

K

I

I

Q

D

R

R

V

V

L

R

T

T

R

R

V

A

W

W

T

T

K

K

V

V

K

K

S

S

G

G

binding (2R)-1-methoxypropan-2-amine: F34 (= F59), D35 (= D60)
binding (2~{R})-1-[(2~{R})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-amine: L91 (= L116), R314 (≠ L339)
binding 1,4-diaminobutane: W9 (= W34), S10 (= S35), Y12 (= Y37), W216 (≠ F241), D219 (= D244), F248 (≠ W273), D250 (= D275), Y286 (= Y311)

1a99A Putrescine receptor (potf) from e. Coli (see paper)
56% identity, 93% coverage: 26:366/367 of query aligns to 1:341/341 of 1a99A

query
sites
1a99A

E

Q

E

K

V

T

V

L

R

H

M

I

Y

Y

N

N

W
|
W

S
|
S

D

D

Y
|
Y

I

I

A

A

E

P

D

D

T

T

L

V

E

A

N

N

F

F

K

E

K

K

E

E

T

T

G

G

I

I

R

K

V

V

I

V

Y

Y

D

D

V

V

F

F

D

D

S

S

N

N

E

E

V

V

V

L

E

E

A

G

K

K

L

L

L

M

S

A

G

G

R

S

S

T

G

G

Y

F

D

D

I

L

V

V

P

P

S

S

N

A

H

S

F

F

L

L

A

E

K

R

Q

Q

I

L

K

T

A

A

G

G

A

V

F

F

K

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

T

P

N

E

F

W

K

K

N

N

L

L

N

D

A

P

D

E

L

L

M

L

K

K

L

L

L

V

E

A

K

K

A

H

D

D

P

P

G

D

N

N

Q

K

Y

F

A

A

V

M

P

P

Y

Y

L

M

W

W

G

A

T

T

N

T

G

G

I

I

G

G

Y

Y

N

N

I

V

N

D

K

K

V

V

K

K

A

A

A

V

V

L

G

G

E

E

D

N

A

A

P

P

P

-

F

V

N

D

S

S

M

W

E

D

L

L

I

I

F

L

N

K

P

P

K

E

Y

N

A

L

E

E

K

K

I

L

S

K

K

S

C

C

G

G

F

V

A

S

M

F

L

L

D

D

S

A

A

P

D

E

M

V

V

F

P

A

Q

T

A

V

M

L

I

N

Y

Y

L

L

G

G

L

K

D

D

P

P

N

N

S

S

T

T

K

K

A

A

E

D

D

D

Y

Y

E

T

-

G

K

P

A

A

G

T

E

D

L

L

E

L

K

K

I

L

R

R

P

P

Y

N

V

I

T

R

Y

Y

F

F

H

H

S

S

R

Q

Y

Y

I

I

S

N

D

D

L

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

Y

I

G

G

F
x
W

S

A

G

G

D
|
D

V

V

F

W

Q

Q

A

A

A

S

A

N

R

R

A

A

D

K

E

E

A

A

G

K

N

N

G

G

N

V

K

N

I

V

G

S

Y

F

S

S

I

I

P

P

K

K

E

E

G

G

A

A

N

M

L

A

W
x
F

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

A

A

D

D

A

A

T

K

N

N

V

K

E

D

N

E

A

A

H

Y

K

Q

L

F

I

L

N

N

Y

Y

L

L

R

R

P

P

E

D

V

V

I

V

A

A

P

H

I

I

S

S

N

D

Y

H

V

V

A

F

Y
|
Y

A

A

N

N

P

A

N

N

D

K

L

A

A

A

Q

T

P

P

L

L

V

V

D

S

E

A

A

E

I

V

R

R

N

E

D

N

P

P

A

G

I

I

Y

Y

P

P

K

A

E

D

V

V

L

R

D

A

K

K

L

L

Y

F

I

T

G

L

E

K

I

V

R

Q

P

D

L

P

K

K

I

I

Q

D

R

R

V

V

L

R

T

T

R

R

V

A

W

W

T

T

K

K

V

V

K

K

S

S

G

G

binding 1,4-diaminobutane: W9 (= W34), S10 (= S35), Y12 (= Y37), W216 (≠ F241), D219 (= D244), F248 (≠ W273), D250 (= D275), Y286 (= Y311)

7xjnA Structure of vcpotd1 in complex with norspermidine
35% identity, 86% coverage: 26:340/367 of query aligns to 2:297/322 of 7xjnA

query
sites
7xjnA

E

D

E

Q

V

E

V

L

R

Y

M

F

Y

Y

N

N

W
|
W

S

S

D
x
E

Y
|
Y

I

I

A

P

E

S

D

E

T

V

L

L

E

E

N

D

F

F

K

T

K

K

E

E

T

T

G

G

I

I

R

K

V

V

I

I

Y

Y

D

S

V

T

F

Y

D

E

S

S

N

N

E

E

V

S

V

M

E

Y

A

A

K

K

L

L

L

K

S

T

G

Q

R

G

S

A

G

G

Y

Y

D

D

I

L

V

V

P

P

S
|
S

N

T

H
x
Y

F

F

L

V

A

S

K

K

Q

M

I

R

K

K

A

E

G

G

A

M

F

L

K

Q

P

E

L

I

D

D

R

H

S

S

K

K

L

L

T

S

N

H

F

F

K

K

N

D

L

L

N

D

A

P

D

N

L

Y

M

L

K

N

L

-

E

K

K

P

A

F

D

D

P

P

G

G

N

N

Q

K

Y

F

A

S

V

I

P

P

Y

Y

L

I

W

W

G

G

T

A

N

T

G

G

I

I

G

G

Y

I

N

N

I

T

N

D

K

M

V

L

K

-

A

-

V

-

G

-

E

-

D

D

A

K

P

K

P

S

F

L

N

K

S

N

M

W

E

G

L

D

I

L

F

W

N

D

P

A

K

K

Y

W

A

A

E

G

K

Q

I

L

S

-

K

-

C

-

G

-

F

-

A

M

M

L

L

M

D

D

S
x
D

A

A

D

R

D
x
E

M

V

V

F

P

H

Q

I

A

A

M

L

I

S

Y

K

L

L

G

G

L

Y

D

S

P

P

N

N

S

T

T

T

K

N

A

P

E

K

D

E

Y

I

E

K

K

A

A

A

G

Y

E

R

L

E

L

L

E

K

K

K

I

L

R

M

P

P

Y

N

V

V

T

L

Y

V

F

F

H

N

S

S

S

D

R

F

Y

P

I

A

S

N

D

P

L

Y

A

L

N

A

G

G

D

E

I

V

C

S

V

L

A

G

Y

M

G

L

F
x
W

S

N

G

G

D

S

V

A

F

Y

Q

M

A

A

A

R

A

Q

R

E

A

-

D

-

E

-

A

-

G

-

N

-

G

G

N

A

K

P

I

I

G

Q

Y

I

S

I

I

W

P

P

K

E

E

K

G

G

A

T

N

I

L

F

W
|
W

F

M

D
|
D

M

S

L

I

A

S

I

I

P

P

A

A

D

G

A

A

T

K

N

N

V

I

E

E

N

A

A

A

H

H

K

K

L

M

I

I

N

D

Y

F

L

L

R

R

P

P

E

E

V

N

I

A

A

A

P

K

I

I

S

A

N

L

Y

E

V

I

A

G

Y
|
Y

A

P

N

T

P

P

N

V

D

K

L

T

A

A

Q

H

P

D

L

L

V

L

D

P

E

K

A

E

I

F

R

A

N

N

D

D

P

P

A

S

I

I

Y

Y

P

P

K

Q

E

S

V

V

L

I

D

D

binding N-(3-aminopropyl)propane-1,3-diamine: W10 (= W34), E12 (≠ D36), Y13 (= Y37), S58 (= S82), Y60 (≠ H84), D143 (≠ S180), E146 (≠ D183), W204 (≠ F241), W230 (= W273), D232 (= D275), Y268 (= Y311)

Sites not aligning to the query:

binding N-(3-aminopropyl)propane-1,3-diamine: 302

P0AFK9 Spermidine/putrescine-binding periplasmic protein; SPBP from Escherichia coli (strain K12) (see 3 papers)
34% identity, 92% coverage: 1:339/367 of query aligns to 1:321/348 of P0AFK9

query
sites
P0AFK9

M
|
M

K
|
K

L
x
K

F
x
W

N
x
S

K
x
R

M
x
H

T
x
L

L
x
A

A
|
A

L
x
G

V
x
A

T
x
L

A
|
A

G
x
L

T
x
G

L
x
M

A
x
S

S
x
A

V
x
A

A
x
H

V
x
A

Q

D

A

D

E

N

V

T

V

L

R

Y

M

F

Y

Y

N

N

W

W

S

T

D
x
E

Y

Y

I

V

A

P

E

P

D

G

T

L

L

L

E

E

N

Q

F

F

K

T

K

K

E

E

T

T

G

G

I

I

R

K

V

V

I

I

Y

Y

D

S

V

T

F

Y

D

E

S

S

N

N

E

E

V

T

V

M

E

Y

A

A

K

K

L

L

L

K

S

T

G

Y

R

K

S

D

G

G

-

A

Y

Y

D

D

I

L

V

V

P

P

S

S

N

T

H
x
Y

F

Y

L

V

A

D

K

K

Q

M

I

R

K

K

A

E

G

G

A

M

F

I

K

Q

P

K

L

I

D

D

R

K

S

S

K

K

L

L

T

T

N

N

F

F

K

S

N

N

L

L

N

D

A

P

D

D

L

M

M

L

K

N

L

-

E

K

K

P

A

F

D

D

P

P

G

N

N

N

Q

D

Y

Y

A

S

V

I

P

P

Y

Y

L

I

W

W

G

G

T

A

N

T

G

A

I

I

G

G

Y

V

N

N

I

G

N

D

K

A

V

V

K

-

A

-

V

-

G

-

E

-

D

D

A

P

P

K

P

S

F

V

N

T

S

S

M

W

E

A

L

D

I

L

F

W

N

K

P

P

K

E

Y

Y

A

-

E

-

K

-

I

-

S

-

K

K

C

G

G

S

F

L

A

L

M

L

L

T

D

D

S
x
D

A
|
A

D
x
R

D
x
E

M

V

V

F

P

Q

Q

M

A

A

M

L

I

R

Y

K

L

L

G

G

L

Y

D

S

P

G

N

N

S

T

T

T

K

D

A

P

E

K

D

E

Y

I

E

E

K

A

A

A

G

Y

E

N

L

E

L

L

E

K

K

K

I

L

R

M

P

P

Y

N

V

V

T

A

Y

A

F

F

H

N

S

S

S

D

R

N

Y

P

I

A

S

N

D

P

L

Y

A

M

N

E

G

G

D

E

I

V

C

N

V

L

A

G

Y

M

G

I

F

W

S

N

G

G

D

S

V

A

F

F

Q

V

A

A

A

R

A

Q

R

A

A

-

D

-

E

-

A

-

G

-

N

-

G

G

N

T

K

P

I

I

G

D

Y

V

S

V

I

W

P

P

K

K

E

E

G

G

A

G

N

I

L

F

W

W

F

M

D

D

M

S

L

L

A

A

I

I

P

P

A

A

D

N

A

A

T

K

N

N

V

K

E

E

N

G

A

A

H

L

K

K

L

L

I

I

N

N

Y

F

L

L

R

R

P

P

E

D

V

V

I

A

A

K

P

Q

I

V

S

A

N

E

Y

T

V

I

A

G

Y

Y

A

P

N

T

P

P

N

N

D

L

L

A

A

A

Q

R

P

K

L

L

V

L

D

S

E

P

A

E

I

V

R

A

N

N

D

D

P

K

A

T

I

L

Y

Y

P

P

P

D

K

A

E

E

V

T

L

I

1:23 (vs. 1:23, 17% identical) signal peptide
E36 (≠ D36) binding
Y85 (≠ H84) binding
DARE 168:171 (≠ SADD 180:183) binding

Sites not aligning to the query:

327 binding

1poy1 Spermidine/putrescine-binding protein complexed with spermidine (dimer form) (see paper)
36% identity, 85% coverage: 28:339/367 of query aligns to 3:296/323 of 1poy1

query
sites
1poy1

V

T

V

L

R

Y

M

F

Y

Y

N

N

W
|
W

S

T

D
x
E

Y
|
Y

I

V

A

P

E

P

D

G

T

L

L

L

E

E

N

Q

F

F

K

T

K

K

E

E

T

T

G

G

I

I

R

K

V

V

I

I

Y

Y

D

S

V

T

F

Y

D

E

S

S

N

N

E

E

V

T

V

M

E

Y

A

A

K

K

L

L

L

K

S

T

G

Y

R

K

S

D

G

G

-

A

Y

Y

D

D

I

L

V

V

P

P

S

S

N

T

H
x
Y

F

Y

L

V

A

D

K

K

Q

M

I

R

K

K

A

E

G

G

A

M

F

I

K

Q

P

K

L

I

D

D

R

K

S

S

K

K

L

L

T

T

N

N

F

F

K

S

N

N

L

L

N

D

A

P

D

D

L

M

M

L

K

N

L

-

E

K

K

P

A

F

D

D

P

P

G

N

N

N

Q

D

Y

Y

A

S

V

I

P

P

Y

Y

L

I

W

W

G

G

T

A

N

T

G

A

I

I

G

G

Y

V

N

N

I

G

N

D

K

A

V

V

K

-

A

-

V

-

G

-

E

-

D

D

A

P

P

K

P

S

F

V

N

T

S

S

M

W

E

A

L

D

I

L

F

W

N

K

P

P

K

E

Y

Y

A

-

E

-

K

-

I

-

S

-

K

K

C

G

G

S

F

L

A

L

M

L

L

T

D

D

S

D

A

A

D

R

D
x
E

M

V

V

F

P

Q

Q

M

A

A

M

L

I

R

Y

K

L

L

G

G

L

Y

D

S

P

G

N

N

S

T

T

T

K

D

A

P

E

K

D

E

Y

I

E

E

K

A

A

A

G

Y

E

N

L

E

L

L

E

K

K

K

I

L

R

M

P

P

Y

N

V

V

T

A

Y

A

F

F

H

N

S

S

S

D

R

N

Y

P

I

A

S

N

D

P

L

Y

A

M

N

E

G

G

D

E

I

V

C

N

V

L

A

G

Y

M

G

I

F

W

S

N

G

G

D

S

V

A

F

F

Q

V

A

A

A

R

A

Q

R

A

A

-

D

-

E

-

A

-

G

-

N

-

G

G

N

T

K

P

I

I

G

D

Y

V

S

V

I

W

P

P

K

K

E

E

G

G

A

G

N

I

L

F

W
|
W

F

M

D
|
D

M

S

L

L

A

A

I

I

P

P

A

A

D

N

A

A

T

K

N

N

V

K

E

E

N

G

A

A

H

L

K

K

L

L

I

I

N

N

Y

F

L

L

R

R

P

P

E

D

V

V

I

A

A

K

P

Q

I

V

S

A

N

E

Y

T

V

I

A

G

Y

Y

A

P

N

T

P

P

N

N

D

L

L

A

A

A

Q

R

P

K

L

L

V

L

D

S

E

P

A

E

I

V

R

A

N

N

D

D

P

K

A

T

I

L

Y

Y

P

P

P

D

K

A

E

E

V

T

L

I

binding spermidine: W9 (= W34), E11 (≠ D36), Y12 (= Y37), Y60 (≠ H84), E146 (≠ D183), W230 (= W273), D232 (= D275)

Sites not aligning to the query:

binding spermidine: 302

4eqbA 1.5 angstrom crystal structure of spermidine/putrescine abc transporter substrate-binding protein potd from streptococcus pneumoniae strain canada mdr_19a in complex with calcium and hepes
30% identity, 86% coverage: 31:346/367 of query aligns to 6:301/323 of 4eqbA

query
sites
4eqbA

M

I

Y

Y

N

N

W

W

S

G

D

D

Y

Y

I

I

A

D

E

P

D

E

T

L

L

L

E

T

N
x
Q

F

F

K

T

K

E

E

E

T

T

G

G

I

I

R

Q

V

V

I

Q

Y

Y

D

E

V

T

F

F

D

D

S

S

N

N

E

E

V

A

V

M

E

Y

A

T

K

K

L

I

L

K

S

Q

G

G

R

G

S

T

G

T

Y

Y

D

D

I

I

V

A

V

I

P

P

S

S

N

E

H

Y

F

M

L

I

A

N

K

K

Q

M

I

K

K

D

A

E

G

D

A

L

F

L

K

V

P

P

L

L

D

D

R

Y

S

S

K

K

L

I

T

E

N

G

F

I

K

E

N

N

L

I

N

G

A

P

D

E

L

F

M

L

K

N

L

-

E

Q

K

S

A

F

D

D

P

P

G

G

N

N

Q

K

Y

F

A

S

V

I

P

P

Y

Y

L

F

W

W

G

G

T

T

N

L

G

G

I

I

G

V

Y

Y

N

N

I

E

N

T

K

M

V

V

K

D

A

E

A

A

V

-

G

-

E

-

D

-

A

-

P

-

F

-

N

-

S

-

M

-

E

-

L

-

I

-

F

-

N

-

P

P

K

E

Y

H

A

W

E

D

K

D

I

L

S

W

K

K

C

L

G

E

F

Y

A

K

-

N

-

S

-

I

-

M

M

L

L

F

D

D

S

G

A

A

D

R

D

E

M

V

V

L

P

G

Q

L

A

G

M

L

I

N

Y

S

L

L

G

G

L

Y

D

S

P

L

N

N

S

S

T

K

K

D

A

P

E

Q

D

Q

Y

L

E

E

K

E

A

T

G

V

E

D

L

K

L

L

E

Y

K

K

I

L

R

T

P

P

Y

N

V

I

T

K

Y

A

F

I

H

V

S

A

S

D

R

E

Y

M

I

K

S

G

D

Y

L

M

A

I

N

Q

G

N

D

N

I

V

C

A

V

I

A

G

Y

V

G

T

F

F

S

S

G

G

D

E

V

A

F

S

Q

Q

A

M

A

L

A

E

R

K

A

N

D

E

E

-

A

-

G

-

N

-

G

-

N

-

K

N

I

L

G

R

Y

Y

S

V

I

V

P

P

K

T

E

E

G

A

A

S

N

N

L

L

W

W

F

F

D

D

M

N

L

M

A

V

I

I

P

P

A

K

D

T

A

V

T

K

N

N

V

Q

E

D

N

S

A

A

H

Y

K

A

L

F

I

I

N

N

Y

F

L

M

L

L

R

K

P

P

E

E

V
x
N

I

A

A

L

P

Q

I

N

S

A

N

E

Y

Y

V

V

A

G

Y

Y

A

S

N

T

P

P

N

N

D

L

L

P

A

A

Q

K

P

E

L

L

V

L

D

P

E

E

A

E

I

T

R

K

N

E

D

D

P

K

A

A

I

F

Y

Y

P

P

P

D

K

V

E

E

V

T

L

M

D

K

K

H

L

L

Y

E

I

V

G

Y

E

E

binding calcium ion: Q20 (≠ N45), N256 (≠ V301)

Sites not aligning to the query:

binding calcium ion: 321, 323

6hlyA Structure in p212121 form of the pbp agtb in complex with agropinic acid from a.Tumefacien r10 (see paper)
23% identity, 80% coverage: 43:334/367 of query aligns to 24:285/320 of 6hlyA

query
sites
6hlyA

L

F

E

D

N

P

F

Y

K

A

K

K

E

A

T

S

G

G

I

V

R

K

V

V

I

T

Y

G

D

T

V

T

F

G

D

T

S

G

N

Y

E

A

V

K

V

V

E

K

A

A

K

M

L

V

L

E

S

S

G

G

R

N

S

V

G

T

Y

W

D

D

I

V

V

I

V

S

P

A

S
x
E

N

S

H

P

F

A

L

F

A

A

K

S

Q

E

I

V

K

K

A

D

G

G

A

L

F

L

K

E

P

P

L

I

D

D

R

Y

S

S

K

V

L

V

T

-

N

-

F

-

K

-

N

-

L

-

N

-

A

-

D

-

L

-

M

-

K

-

L

-

E

-

K

K

A

A

D

D

-

N

-

V

P

P

G

E

N

N

-

F

-

R

-

T

Q

K

Y

Y

A

G

V

V

P

G

Y

Y

L
x
M

-

V

W
x
F

G

G

T

T

N

N

G

-

I

L

G

A

Y

W

N

N

I

K

N

D

K

K

V

F

K

P

A

N

A

G

V

V

G

-

E

-

D

-

A

-

P

-

F

-

N

-

S

T

M

P

E

A

L

Q

I

F

F

F

N

D

P

P

K

N

Y

V

A

-

E

-

K

-

I

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S

-

K

K

C

G

G

R

F

R

A

V

M

L

L

P

D

S

S

D

A

A

D

T

D

Y

M

S

V

L

P

E

Q

F

A

A

M

L

I

M

Y

G

L

D

G

G

L

V

D

K

P

P

N

A

S

D

T

L

K

Y

A

P

E

L

D

D

Y

V

E

K

K

R

A

A

G

L

E

K

L

V

L

I

E

D

K

R

I

V

R

K

P

D

Y

Q

V

V

T

I

Y

G

F

Y

H

K

S

G

S
x
A

R

S

Y

D

I

I

S

Q

D

A

L

L

-

M

A

Q

N

Q

G

G

D

E

I

A

C

D

V

I

A

V

Y

Y

G

A

F

G

S

T

G

G

D
x
R

V

I

F

-

Q

-

A

-

A

K

R

N

A

A

D

I

E

K

A

A

G

G

N

-

G

-

N

-

K

-

I

A

G

N

Y

W

S

S

I

Y

P

S

K

W

E

E

G

G

A

A

N

L

L

A

W

D

F

T

D
x
E

M

Y

L

W

A

A

I

V

P

P

A

K

D

G

A

A

T

P

N

H

V

A

E

A

N

E

A

A

H

M

K

K

L

F

I

I

N

N

Y

F

L

A

L

V

R

Q

P

A

E

E

V

P

I

Q

A

A

P

E

I

L

S

T

N

R

Y

V

V

I

A

A

Y
|
Y

A

G

N

P

P

T

N

N

D

V

L

D

A

A

Q

L

P

R

L

L

V

L

D

D

E

P

A

A

I

V

R

A

N

K

D

D

P

L

A

P

I

S

Y

Y

P

P

binding agropinic acid: E63 (≠ S82), M102 (≠ L131), F104 (≠ W132), A182 (≠ S224), R203 (≠ D244), E226 (≠ D275), Y262 (= Y311)

Sites not aligning to the query:

binding agropinic acid: 11, 15

Query Sequence

>200448 FitnessBrowser__MR1:200448
MKLFNKMTTLALVTAGTLASVAVQAEEVVRMYNWSDYIAEDTLENFKKETGIRVIYDVFD
SNEVVEAKLLSGRSGYDIVVPSNHFLAKQIKAGAFKPLDRSKLTNFKNLNADLMKLLEKA
DPGNQYAVPYLWGTNGIGYNINKVKAAVGEDAPPFNSMELIFNPKYAEKISKCGFAMLDS
ADDMVPQAMIYLGLDPNSTKAEDYEKAGELLEKIRPYVTYFHSSRYISDLANGDICVAYG
FSGDVFQAAARADEAGNGNKIGYSIPKEGANLWFDMLAIPADATNVENAHKLINYLLRPE
VIAPISNYVAYANPNDLAQPLVDEAIRNDPAIYPPKEVLDKLYIGEIRPLKIQRVLTRVW
TKVKSGQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory