SitesBLAST

G51 (≠ V76) mutation to S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
A54 (≠ Y79) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
K81 (≠ G106) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.
S202 (≠ G237) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
I332 (≠ V370) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
M342 (≠ I380) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.

1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
23% identity, 71% coverage: 93:397/428 of query aligns to 57:359/364 of 1ng3A

query
sites
1ng3A

E

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F

binding acetylamino-acetic acid: Y246 (= Y285), R302 (≠ F342), R329 (≠ G367)
binding flavin-adenine dinucleotide: V174 (= V210), S202 (≠ G237), G203 (≠ N238), W205 (≠ Y240), F209 (≠ L244), G300 (= G340), R302 (≠ F342), H327 (≠ C365), R329 (≠ G367), N330 (≠ H368), G331 (= G369), I332 (≠ V370)
binding phosphate ion: R89 (≠ D121), R254 (= R297)

Sites not aligning to the query:

active site: 47, 48, 49
binding flavin-adenine dinucleotide: 11, 13, 15, 33, 34, 35, 41, 42, 43, 46, 47, 48, 49

O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
23% identity, 71% coverage: 93:397/428 of query aligns to 57:359/369 of O31616

query
sites
O31616

E

E

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Y

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V174 (= V210) binding FAD
H244 (≠ Y278) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
R302 (≠ F342) binding substrate
327:333 (vs. 365:371, 14% identical) binding FAD
R329 (≠ G367) binding substrate

Sites not aligning to the query:

14:15 binding FAD
34:35 binding FAD
42:43 binding FAD
47:49 binding FAD
51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.

3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
23% identity, 71% coverage: 93:397/428 of query aligns to 57:359/364 of 3if9A

query
sites
3if9A

E

E

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F

binding flavin-adenine dinucleotide: P173 (≠ A209), V174 (= V210), S202 (≠ G237), G203 (≠ N238), W205 (≠ Y240), F209 (≠ L244), G300 (= G340), R302 (≠ F342), H327 (≠ C365), F328 (≠ S366), R329 (≠ G367), N330 (≠ H368), G331 (= G369), I332 (≠ V370)
binding glycolic acid: Y246 (= Y285), R302 (≠ F342), R329 (≠ G367)

Sites not aligning to the query:

active site: 47, 48, 49
binding flavin-adenine dinucleotide: 11, 13, 15, 34, 35, 42, 43, 46, 47, 48, 49

5i39A High resolution structure of l-amino acid deaminase from proteus vulgaris with the deletion of the specific insertion sequence (see paper)
22% identity, 85% coverage: 29:392/428 of query aligns to 24:374/383 of 5i39A

query
sites
5i39A

E

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G

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G

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K

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L

P

L

F

D

K

G

T

K

R

G

I

I

I

R

E

S

G

V

A

V

E

N

L

T

N

E

Q

R

R

Y

L

V

R

G

G

G

A

M

T

L

T

D

D

-

W

-

K

-

I

-

A

-

G

-

F

-

E

K

E

S

D

G

S

G

G

H

S

I

F

H

D

P

P

L

E

N

V

L

A

A

T

L

F

G

V

E

M

A

A

R

E

A

Y

V

A

E

K

S

K

L

M

G

G

G

V

K

R

I

I

F

Y

E

T

D

Q

S

C

A
|
A

V
x
A

-

R

-

G

L

L

R

E

V

T

D

Q

E

A

G

G

D

V

N

I

P

S

V

D

V

V

H

V

T

T

A

E

T

K

G

G

S

A

V

I

K

K

A

T

K

S

F

Q

V

V

A

A

G
|
G

N
x
G

A

V

Y
x
W

L

S

G

R

K

L

L

F

M

M

P

Q

E

N

L

L

Q

N

-

V

-

D

A

V

K

P

S

T

M

L

P

P

C

A

-

Y

-
x
Q

G

S

T
x
Q

Q

Q

V

L

I

I

T

S

T

G

E

S

P

P

L

-

D

-

E

-

D

-

L

-

A

-

S

-

L

-

P

-

Q

-

D

-

Y

-

C

T

V

A

E

P

D

G

C

G

N

N

Y

V

L

A

L

L

D
x
P

-

G

-

I

Y

F

F

F

R

R

L

E

S

Q

G

A

D

D

K

G

R

T

M

Y

I

A

Y

T

G

S

G

P

G

R

V

V

V

I

Y

V

G

A

A

L

R

P

D

D

P

L

A

P

D

E

I

L

E

N

S

A

L

S

I

-

I

L

P

E

N

K

M

L

L

K

K

A

T

E

F

F

P

P

Q

A

L

F

K

K

G

E

V

S

K

K

V

L

D

I

Y

D

A

Q

W

W

T

S

G
|
G

N

A

F
x
M

L

A

L

I

T

A

L

P

S

D

R

E

L

N

P

P

Q

I

V

I

G

S

R

E

I

V

G

K

K

E

N

Y

I

P

-

G

Y

L

Y

V

S

I

Q

N

G

T

C

A

S
x
T

G
|
G

H
x
W

G
|
G

V
x
M

T
|
T

Y

E

T

S

H

P

L

V

A

S

G

A

K

E

L

L

I

T

A

A

E

D

M

L

L

L

N

L

G

G

Q

K

A

K

T

P

R

V

F

L

D

D

active site: F66 (≠ N71), Q69 (= Q74), A70 (≠ I75), Q248 (vs. gap), P267 (≠ D288)
binding flavin-adenine dinucleotide: V30 (≠ I35), G31 (= G36), G33 (= G38), I34 (≠ Y39), L35 (≠ T40), V53 (≠ L58), E54 (≠ D59), K55 (≠ A60), Q62 (≠ A67), S63 (= S68), F66 (≠ N71), Y67 (≠ G72), Q69 (= Q74), A196 (= A209), A197 (≠ V210), G226 (= G237), G227 (≠ N238), W229 (≠ Y240), Q248 (vs. gap), Q250 (≠ T257), G321 (= G340), M323 (≠ F342), T348 (≠ S366), G349 (= G367), W350 (≠ H368), G351 (= G369), M352 (≠ V370), T353 (= T371)

5hxwA L-amino acid deaminase from proteus vulgaris (see paper)
25% identity, 55% coverage: 29:264/428 of query aligns to 16:249/433 of 5hxwA

query
sites
5hxwA

E

Q

A

A

D

D

V

V

C

V

V

V

I
x
V

G
|
G

A

A

G
|
G

Y
x
I

T
x
L

G

G

L

I

S

M

A

T

A

A

L

I

H

N

L

L

L

V

E

E

L

R

G

G

F

L

S

S

V

V

V

I

L

V

D
x
E

A
x
K

A

G

R

N

I

I

G

A

W

G

G
x
E

A
x
Q

S
|
S

G

S

R
|
R

N
x
F

G
x
Y

G
|
G

Q
|
Q

I
x
A

V

I

N

S

S

Y

Y

K

S

M

R

P

D

D

I

E

D

T

T

F

I

L

E

L

K

H

T

H

V

L

G

G

K

K

E

H

Q

R

A

-

K

-

L

-

F

-

G

-

K

-

M

-

A

-

F

-

E

-

G

-

R

-

I

-

I

W

R

R

E

E

R

M

I

N

A

A

K

K

Y

V

N

G

I

I

S

D

C

T

D

T

L

Y

K

R

-

T

D

Q

G

G

G

R

V

V

F

E

A

V

A

P

M

L

N

D

E

E

K

E

Q

D

M

L

G

V

H

N

L

V

R

R

H

-

Q

-

K

K

Q

W

L

I

W

D

E

E

S

R

H

S

G

K

H

N

M

V

G

G

Q

S

L

D

E

I

L

P

L

F

D

K

G

T

K

R

G

I

I

I

R

E

S

G

V

A

V

E

N

L

T

N

E

Q

R

R

Y

L

V

R

G

G

G

A

M

T

L

T

D

D

-

W

-

K

-

I

-

A

-

G

-

F

-

E

K

E

S

D

G

S

G

G

H

S

I

F

H

D

P

P

L

E

N

V

L

A

A

T

L

F

G

V

E

M

A

A

R

E

A

Y

V

A

E

K

S

K

L

M

G

G

G

V

K

R

I

I

F

Y

E

T

D

Q

S

C

A
|
A

V
x
A

-

R

-

G

L

L

R

E

V

T

D

Q

E

A

G

G

D

V

N

I

P

S

V

D

V

V

H

V

T

T

A

E

T

K

G

G

S

A

V

I

K

K

A

T

K

S

F

Q

V

V

A

A

G
|
G

N
x
G

A

V

Y
x
W

L

S

G

R

K

L

L

F

M

M

P

Q

E

N

L

L

Q

N

-

V

-

D

A

V

K

P

S

T

M

L

P

P

C

A

-

Y

-
x
Q

G

S

T
x
Q

Q

Q

V

L

I

I

T

S

T

G

E

S

P

P

active site: F58 (≠ N71), Q61 (= Q74), A62 (≠ I75), Q240 (vs. gap)
binding flavin-adenine dinucleotide: V22 (≠ I35), G23 (= G36), G25 (= G38), I26 (≠ Y39), L27 (≠ T40), E46 (≠ D59), K47 (≠ A60), E53 (≠ G66), Q54 (≠ A67), S55 (= S68), R57 (= R70), F58 (≠ N71), Y59 (≠ G72), G60 (= G73), Q61 (= Q74), A188 (= A209), A189 (≠ V210), G218 (= G237), G219 (≠ N238), W221 (≠ Y240), Q240 (vs. gap), Q242 (≠ T257)

Sites not aligning to the query:

active site: 284, 288
binding cetyl-trimethyl-ammonium: 291, 294, 310, 311, 317, 318, 320, 373, 379, 399, 400
binding flavin-adenine dinucleotide: 331, 371, 373, 398, 399, 400, 401, 402, 403

Query Sequence

>200452 FitnessBrowser__MR1:200452
MSAIPHTGSYYAASANDKVERARLQESIEADVCVIGAGYTGLSAALHLLELGFSVVVLDA
ARIGWGASGRNGGQIVNSYSRDIDTIEKTVGKEQAKLFGKMAFEGGRIIRERIAKYNISC
DLKDGGVFAAMNEKQMGHLRHQKQLWESHGHMGQLELLDGKGIRSVVNTERYVGGMLDKS
GGHIHPLNLALGEARAVESLGGKIFEDSAVLRVDEGDNPVVHTATGSVKAKFVVVAGNAY
LGKLMPELQAKSMPCGTQVITTEPLDEDLAASLLPQDYCVEDCNYLLDYFRLSGDKRMIY
GGGVVYGARDPADIESLIIPNMLKTFPQLKGVKVDYAWTGNFLLTLSRLPQVGRIGKNIY
YSQGCSGHGVTYTHLAGKLIAEMLNGQATRFDAFAALPHYPFPGGHALRVPFSALGAWYY
SLRDKLGV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory