SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 200453 FitnessBrowser__MR1:200453 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
71% identity, 100% coverage: 1:482/482 of query aligns to 1:482/482 of P25526

query
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P25526

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WN 156:157 (= WN 156:157) binding NADP(+)
KPAS 180:183 (≠ KPAP 180:183) binding NADP(+)
GS 233:234 (= GS 233:234) binding NADP(+)
L256 (= L256) binding NADP(+)
E386 (= E386) binding NADP(+)

3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
71% identity, 100% coverage: 3:482/482 of query aligns to 2:481/481 of 3jz4A

query
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3jz4A

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active site: N156 (= N157), K179 (= K180), E254 (= E255), C288 (= C289), E385 (= E386), E462 (= E463)
binding nadp nicotinamide-adenine-dinucleotide phosphate: P154 (= P155), W155 (= W156), K179 (= K180), A181 (= A182), S182 (≠ P183), A212 (= A213), G216 (= G217), G232 (= G233), S233 (= S234), I236 (≠ V237), C288 (= C289), K338 (= K339), E385 (= E386), F387 (= F388)

8c54A Cryo-em structure of nadh bound sla dehydrogenase rlgabd from rhizobium leguminosarum bv. Trifolii srd1565 (see paper)
62% identity, 99% coverage: 3:480/482 of query aligns to 2:479/482 of 8c54A

query
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8c54A

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L

I

I

N

D

A

E

A

K

A

A

V

V

E

A

K

K

V

M

Q

E

S

E

H

H

L

I

E

A

D

D

A

A

I

M

K

A

K

N

G

G

A

G

T

T

V

V

L

L

A

T

G

G

K

K

V

R

H

S

E

V

L

L

G

G

N

T

F

F

E

E

P

P

T

T

V

V

L

V

T

T

N

G

A

V

D

T

K

Q

S

T

M

M

R

K

V

V

A

A

R

K

E

E

T

T

F

F

G

A

P

P

L

L

A

A

P

P

L

I

F

I

K

S

F

F

N

K

D

H

V

E

D

N

D

D

V

V

I

I

K

A

Q

M

A

A

N

N

D

D

T

S

E

E

F

F

G

G

L

L

A

A

A

S

Y

Y

F

F

Y

Y

G

A

R

K

D

D

I

M

S

A

L

R

V

I

W

W

K

R

V

V

A

A

E

E

S

A

L

L

E

E

Y

A

G

G

M

M

V

V

G

G

V

V

N

N

T

T

G

G

L

M

I

I

S

A

T

N

E

E

V

M

A

A

P

P

F

F

G

G

M

I

K

K

S

Q

S

S

G

G

L

T

G

G

R

R

E

E

G

G

S

S

K

K

Y

Y

G

G

I

I

E

E

E

G

Y

F

L

L

E

E

I

L

K

K

Y

Y

I

V

C

A

M

L

binding 1,4-dihydronicotinamide adenine dinucleotide: I152 (= I153), T153 (= T154), P154 (= P155), K179 (= K180), A212 (= A213), K213 (≠ I214), F230 (= F231), T231 (= T232), G232 (= G233), S233 (= S234), V236 (= V237), W239 (≠ K240), G256 (= G257)

8of1A Structure of aldh5f1 from moss physcomitrium patens in complex with NAD+ in the contracted conformation
59% identity, 99% coverage: 3:480/482 of query aligns to 20:497/505 of 8of1A

query
sites
8of1A

L

L

N

N

D

E

P

A

S

G

L

L

L

F

R

K

Q

S

Q

Q

C

G

Y

L

I

I

N

G

G

D

Q

K

W

W

C

V

D

D

A

A

N

E

S

N

K

G

E

H

T

T

V

L

A

P

I

V

T

N

N

N

P

P

A

A

T

T

G

G

A

E

V

I

I

L

A

T

C

S

V

V

P

P

V

F

M

M

G

G

Q

K

A

R

E

E

T

A

Q

E

A

K

A

A

I

I

A

A

E

S

A

Q

A

A

L

F

P

T

A

S

W

W

R

S

A

K

L

R

T

T

A

A

K

N

E

D

R

R

G

S

A

K

K

I

L

L

R

R

R

Q

W

W

F

F

E

N

L

L

N

I

E

K

N

N

S

K

D

D

L

L

A

G

L

K

L

L

M

I

T

V

S

L

E

E

Q

Q

G

G

K

K

P

P

L

L

T

A

E

E

A

A

K

V

G

G

E

E

V

I

T

V

Y

Y

A

G

A

A

S

A

F

F

I

V

E

E

W

Y

F

Y

A

A

E

E

A

A

K

K

R

R

I

V

Y

Y

G

G

D

D

T

I

I

I

P

P

G

S

H

P

Q

F

G

P

D

E

K

K

R

R

I

M

M

L

V

V

I

M

K

K

Q

Q

P

P

V

V

G

G

V

V

T

V

A

A

I
|
I

T
x
A

P
|
P

W
|
W

N

N

F

F

P

P

A

L

A

A

M

M

I

I

T

T

R

R

K

K

A

V

A

A

P

P

A

A

L

L

A

A

G

G

C

C

T

T

M

V

V

V

V

I

K
|
K

P

P

A

S

P

E

Q

L

T

T

P

P

F

L

T

T

A

A

L

L

A

A

L

A

A

A

V

E

L

L

A

A

E

L

R

Q

A

A

G

G

I

I

P

P

A

P

G

G

V

V

F

V

S

N

V

V

I

V

T

M

G

G

D

D

A
|
A

I

K

A

G

I

I

G

G

N

D

E

A

M

M

C

L

T

D

N

S

P

T

I

E

V

V

R

R

K

K

L

I

S

T

F
|
F

T

T

G
|
G

S
|
S

T

T

N

G

V
|
V

G

G

I

K

K
x
M

L

L

M

L

A

A

Q

G

C

A

A

G

P

K

T

T

L

V

K

K

L

V

S

S

L

L

E

E

L
|
L

G

G

N

N

A

A

P

P

F

C

I

I

V

V

F

F

D

D

A

A

N

N

I

L

D

D

A

V

A

A

V

V

E

K

G

G

A

V

M

L

I

A

A

G

K

K

Y

Y

R

R

N

N

A

S

G

G

Q

Q

T

T

C
|
C

V

V

C

C

A

I

N

N

R

K

I

I

Y

F

V

V

Q

Q

A

D

G

G

V

I

Y

Y

D

D

E

K

F

F

A

A

E

E

K

A

L

F

S

A

M

K

A

A

V

V

A

S

K

G

L

L

K

R

V

A

G

G

E

N

G

G

I

L

I

E

A

P

G

G

V

I

T

T

T

Q

G

G

P

P

L

L

I

I

N

N

A

E

A

T

A

A

V

L

E

E

K
|
K

V

V

Q

E

S

R

H

H

L

V

E

Q

D

D

A

A

I

V

K

S

K

K

G

G

A

A

T

K

V

V

L

L

A

V

G

G

K

K

V

R

H

H

E

S

L

L

G

G

G

R

N

T

F

F

F

Y

E

E

P

P

T

T

V

I

L

L

T

G

N

N

A

A

D

S

K

D

S

E

M

M

R

L

V

I

A

F

R

R

E

E

E
|
E

T

V

F
|
F

G

G

P

P

L

V

A

A

P

P

L

L

F

V

K

R

F

F

N

N

D

T

V

D

D

E

V

A

I

I

K

K

Q

L

A

A

N

N

D

N

T

S

E

E

F

F

G

G

L

L

A

A

Y

Y

F

A

Y

F

G

T

R

E

D

N

I

I

S

T

L

R

V

G

W

W

K

R

V

V

A

A

E

E

S

S

L

L

E

E

Y

F

G

G

M

M

V

V

G

G

V

L

N

N

T

E

G

G

L

L

I

I

S

S

T

T

E

E

V

V

A

A

P

P

F

F

G

G

M

M

K

K

S

Q

S

S

G

G

L

L

G

G

R

R

E

E

G

G

S

S

K

K

Y

Y

G

G

I

L

E

D

E

E

Y

Y

L

L

E

E

I

M

K

K

Y

Y

I

V

C

C

M

L

binding nicotinamide-adenine-dinucleotide: I170 (= I153), A171 (≠ T154), P172 (= P155), W173 (= W156), K197 (= K180), A230 (= A213), F248 (= F231), G250 (= G233), S251 (= S234), V254 (= V237), M257 (≠ K240), L273 (= L256), C306 (= C289), K356 (= K339), E403 (= E386), F405 (= F388)

P51649 Succinate-semialdehyde dehydrogenase, mitochondrial; Aldehyde dehydrogenase family 5 member A1; NAD(+)-dependent succinic semialdehyde dehydrogenase; EC 1.2.1.24 from Homo sapiens (Human) (see 5 papers)
55% identity, 98% coverage: 7:479/482 of query aligns to 57:531/535 of P51649

query
sites
P51649

S

A

L

L

R

R

Q

T

Q

D

C

S

Y

F

I

V

N

G

G

G

Q

R

W

W

C

L

D

P

A

A

N

A

S

A

K

-

E

-

T

T

V

F

A

P

I

V

T

Q

N

D

P

P

A

A

T

S

G

G

A

A

V

A

I

L

A

G

C

M

V

V

P

A

V

D

M
x
C

G

G

Q

V

A

R

E

E

T

A

Q

R

A

A

I

V

A

R

A

A

E

Y

A

E

A

A

L

F

P

C

A

R

W

W

R

R

A

E

L

V

T

S

A

A

K

K

E

E

R

R

G

S

A

S

K

L

L

L

R

R

R

K

W

W

F

Y

E

N

L

L

L

M

N

I

E

Q

N

N

S

K

D

D

L

L

A

A

L

R

L

I

M

I

T

T

S

A

E

E

Q

S

G

G

K

K

P

P

L

L

T

K

E

E

A

A

K

H

G

G

E

E

V

I

T

L

Y

Y

A

S

A

A

S

F

F

F

I

L

E

E

W

W

F

F

A

S

E

E

A

A

K

R

R

R

I

V

Y

Y

G
|
G

D

D

T

I

I

I

P
x
H

G

T

H
x
P

Q

A

G

K

D

D

K

R

R

R

I

A

M

L

V

V

I

L

K

K

Q

Q

P

P

V

I

G

G

V

V

T

A

A

A

A

V

I

I

T

T

P

P

W

W

N

N

F

F

P

P

A

S

A

A

M

M

I

I

T

T

R
|
R

K

K

A

V

A

G

P

A

A

A

L

L

A

A

G

G

C
|
C

T

T

M

V

V

V

K
|
K

P
|
P

A
|
A

P
x
E

Q

D

T
|
T

P

P

F

F

T

S

A
|
A

L

L

A

A

L

L

A

A

V

E

L

L

A

A

E

S

R

Q

A

A

G

G

I

I

P

P

A

S

G

G

V

V

F

Y

S
x
N

V

V

I

I

-

P

-

C

-

S

T

R

G

K

D

N

A

A

I

K

A

E

I

V

G
|
G

N

E

E

A

M

I

C

C

T

T

N

D

P

P

I

L

V

V

R

S

K

K

L

I

S

S

F

F

T

T

G
|
G

S
|
S

T
|
T

N
x
T

V
x
T

G
|
G

I

K

K

I

L

L

M

L

A

H

Q

H

C

A

A

A

P

N

T

S

L

V

K

K

K

R

L

V

S

S

L

M

E

E

L

L

G

G

N

L

A

A

P

P

F

F

I

I

V

V

F

F

D

D

D

S

A

A

N

N

I

V

D

D

A

Q

A

A

V

V

E

A

G

G

A

A

M

M

I

A

A

S

K

K

Y

F

R
|
R

N
|
N

A

T

G

G

Q

Q

T

T

C
|
C

V

V

C
|
C

A

S

N

N

R

Q

I

F

Y

L

V

V

Q

Q

A

R

G

G

V

I

Y

H

D

D

E

A

F

F

A

V

E

K

K

A

L

F

S

A

M

E

A

A

V

M

A

K

K

K

-

N

L

L

K

R

V

V

G

G

E
x
N

G

G

I

F

I

E

A

E

G

G

V

T

T

T

T

Q

G

G

P
|
P

L

L

I

I

N

N

A

E

A

K

A

A

V

V

E

E

K

K

V

V

Q

E

S

K

H

Q

L

V

E

N

D

D

A

A

I

V

K

S

K

K

G

G

A

A

T

T

V
|
V

L

V

A

T

G
|
G

G

G

K

K

V

R

H

H

E

Q

L

L

G

G

G

K

N

N

F

F

E

E

P

P

T

T

V

L

L

L

T

C

N

N

A

V

D

T

K

Q

S

D

M

M

R

L

V

C

A

T

R

H

E

E

T

T

F

F

G

G

P

P

L

L

A

A

P

P

L

V

F

I

K

K

F

F

N

D

D

T

V

E

D

E

V

A

I

I

K

A

Q

I

A

A

N

N

D

A

T

A

E

D

F

V

G

G

L

L

A

A

A

G

Y

Y

F

F

Y

Y

G

S

R

Q

D

D

I

P

S

A

L

Q

V

I

W

W

K

R

V

V

A

A

E

E

S

Q

L

L

E

E

Y

V

G

G

M

M

V

V

G

G

V

V

N

N

T

E

G

G

L

L

I

I

S
|
S

T

S

E

V

V

E

A

C

P

P

F

F

G

G

M

V

K

K

S

Q

S

S

G

G

L

L

G

G

R

R

E

E

G

G

S

S

K

K

Y

Y

G

G

I

I

E

D

E

E

Y

Y

L

L

E

E

I

L

K

K

Y

Y

I

V

C

C

C93 (≠ M45) to F: in SSADHD; 3% of activity; dbSNP:rs765561257
G176 (= G128) to R: in SSADHD; <1% of activity; dbSNP:rs72552281
H180 (≠ P132) to Y: 83% of activity; dbSNP:rs2760118
P182 (≠ H134) to L: 48% of activity; dbSNP:rs3765310
R213 (= R165) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
C223 (= C175) to Y: in SSADHD; 5% of activity; dbSNP:rs72552282
KPAE 228:231 (≠ KPAP 180:183) binding NAD(+)
T233 (= T185) to M: in SSADHD; 4% of activity; dbSNP:rs1326526453
A237 (= A189) to S: 65% of activity; dbSNP:rs62621664
N255 (≠ S207) to S: in SSADHD; 17% of activity; dbSNP:rs145087265
G268 (= G217) to E: in SSADHD; <1% of activity; dbSNP:rs375628463
GSTTTG 284:289 (≠ GSTNVG 233:238) binding NAD(+)
R334 (= R283) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
N335 (= N284) to K: in SSADHD; 1% of activity; dbSNP:rs72552283
C340 (= C289) modified: Disulfide link with 342, In inhibited form
C342 (= C291) modified: Disulfide link with 340, In inhibited form; mutation to A: Loss of regulation by redox state.
N372 (≠ E320) natural variant: N -> S
P382 (= P330) to L: in SSADHD; 2% of activity
V406 (= V354) to I: in dbSNP:rs143741652
G409 (= G357) to D: in SSADHD; <1% of activity; dbSNP:rs118203984
S498 (= S446) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.

Sites not aligning to the query:

36 G → R: no effect on succinate-semialdehyde dehydrogenase activity; dbSNP:rs4646832
533 G → R: in SSADHD; <1% of activity; dbSNP:rs72552284

2w8rA The crystal structure of human ssadh in complex with NAD+ (see paper)
55% identity, 98% coverage: 7:479/482 of query aligns to 7:481/485 of 2w8rA

query
sites
2w8rA

S

A

L

L

R

R

Q

T

Q

D

C

S

Y

F

I

V

N

G

G

G

Q

R

W

W

C

L

D

P

A

A

N

A

S

A

K

-

E

-

T

T

V

F

A

P

I

V

T

Q

N

D

P

P

A

A

T

S

G

G

A

A

V

A

I

L

A

G

C

M

V

V

P

A

V

D

M

C

G

G

Q

V

A

R

E

E

T

A

Q

R

A

A

I

V

A

R

A

A

E

Y

A

E

A

A

L

F

P

C

A

R

W

W

R

R

A

E

L

V

T

S

A

A

K

K

E

E

R

R

G

S

A

S

K

L

L

L

R

R

R

K

W

W

F

Y

E

N

L

L

L

M

N

I

E

Q

N

N

S

K

D

D

L

L

A

A

L

R

L

I

M

I

T

T

S

A

E

E

Q

S

G

G

K

K

P

P

L

L

T

K

E

E

A

A

K

H

G

G

E

E

V

I

T

L

Y

Y

A

S

A

A

S

F

F

F

I

L

E

E

W

W

F

F

A

S

E

E

A

A

K

R

R

R

I

V

Y

Y

G

G

D

D

T

I

I

I

P

H

G

T

H

P

Q

A

G

K

D

D

K

R

R

R

I

A

M

L

V

V

I

L

K

K

Q

Q

P

P

V

I

G

G

V

V

T

A

A

A

A

V

I

I

T
|
T

P

P

W

W

N
|
N

F

F

P

P

A

S

A

A

M

M

I

I

T

T

R

R

K

K

A

V

A

G

P

A

A

A

L

L

A

A

G

G

C

C

T

T

M

V

V

V

K
|
K

P

P

A

A

P

E

Q

D

T

T

P

P

F

F

T

S

A

A

L

L

A

A

L

L

A

A

V

E

L

L

A

A

E

S

R

Q

A

A

G

G

I

I

P

P

A

S

G

G

V

V

F

Y

S

N

V

V

I

I

-

P

-

C

-

S

T

R

G

K

D

N

A
|
A

I

K

A

E

I

V

G

G

N

E

E

A

M

I

C

C

T

T

N

D

P

P

I

L

V

V

R

S

K

K

L

I

S

S

F

F

T

T

G

G

S
|
S

T

T

N

T

V
x
T

G

G

I

K

K

I

L

L

M

L

A

H

Q

H

C

A

A

A

P

N

T

S

L

V

K

K

K

R

L

V

S

S

L

M

E
|
E

L

L

G

G

N

L

A

A

P

P

F

F

I

I

V

V

F

F

D

D

D

S

A

A

N

N

I

V

D

D

A

Q

A

A

V

V

E

A

G

G

A

A

M

M

I

A

A

S

K

K

Y

F

R

R

N

N

A

T

G

G

Q

Q

T

T

C
x
A

V

V

C

C

A

S

N

N

R

Q

I

F

Y

L

V

V

Q

Q

A

R

G

G

V

I

Y

H

D

D

E

A

F

F

A

V

E

K

K

A

L

F

S

A

M

E

A

A

V

M

A

K

K

K

-

N

L

L

K

R

V

V

G

G

E

N

G

G

I

F

I

E

A

E

G

G

V

T

T

T

T

Q

G

G

P

P

L

L

I

I

N

N

A

E

A

K

A

A

V

V

E

E

K

K

V

V

Q

E

S

K

H

Q

L

V

E

N

D

D

A

A

I

V

K

S

K

K

G

G

A

A

T

T

V

V

L

V

A

T

G

G

K

K

V

R

H

H

E

Q

L

L

G

G

G

K

N

N

F

F

E

E

P

P

T

T

V

L

L

L

T

C

N

N

A

V

D

T

K

Q

S

D

M

M

R

L

V

C

A

T

R

H

E

E

E
|
E

T

T

F

F

G

G

P

P

L

L

A

A

P

P

L

V

F

I

K

K

F

F

N

D

D

T

V

E

D

E

V

A

I

I

K

A

Q

I

A

A

N

N

D

A

T

A

E

D

F

V

G

G

L

L

A

A

A

G

Y

Y

F

F

Y

Y

G

S

R

Q

D

D

I

P

S

A

L

Q

V

I

W

W

K

R

V

V

A

A

E

E

S

Q

L

L

E

E

Y

V

G

G

M

M

V

V

G

G

V

V

N

N

T

E

G

G

L

L

I

I

S

S

T

S

E

V

V

E

A

C

P

P

F

F

G

G

M

V

K

K

S

Q

S

S

G

G

L

L

G

G

R

R

E
|
E

G

G

S

S

K

K

Y

Y

G

G

I

I

E

D

E

E

Y

Y

L

L

E

E

I

L

K

K

Y

Y

I

V

C

C

active site: N155 (= N157), K178 (= K180), E256 (= E255), A290 (≠ C289), E388 (= E386), E465 (= E463)
binding adenosine-5'-diphosphate: T152 (= T154), K178 (= K180), A214 (= A213), S235 (= S234), T238 (≠ V237)

2w8qA The crystal structure of human ssadh in complex with ssa. (see paper)
55% identity, 98% coverage: 7:479/482 of query aligns to 7:481/485 of 2w8qA

query
sites
2w8qA

S

A

L

L

R

R

Q

T

Q

D

C

S

Y

F

I

V

N

G

G

G

Q

R

W

W

C

L

D

P

A

A

N

A

S

A

K

-

E

-

T

T

V

F

A

P

I

V

T

Q

N

D

P

P

A

A

T

S

G

G

A

A

V

A

I

L

A

G

C

M

V

V

P

A

V

D

M

C

G

G

Q

V

A

R

E

E

T

A

Q

R

A

A

I

V

A

R

A

A

E

Y

A

E

A

A

L

F

P

C

A

R

W

W

R

R

A

E

L

V

T

S

A

A

K

K

E

E

R

R

G

S

A

S

K

L

L

L

R

R

R

K

W

W

F

Y

E

N

L

L

L

M

N

I

E

Q

N

N

S

K

D

D

L

L

A

A

L

R

L

I

M

I

T

T

S

A

E

E

Q

S

G

G

K

K

P

P

L

L

T

K

E

E

A

A

K

H

G

G

E

E

V

I

T

L

Y

Y

A

S

A

A

S

F

F

F

I

L

E

E

W

W

F

F

A

S

E

E

A

A

K

R

R

R

I

V

Y

Y

G

G

D

D

T

I

I

I

P

H

G

T

H

P

Q

A

G

K

D

D

K

R

R

R

I

A

M

L

V

V

I

L

K

K

Q

Q

P

P

V

I

G

G

V

V

T

A

A

A

A

V

I

I

T

T

P

P

W

W

N
|
N

F

F

P

P

A

S

A

A

M

M

I

I

T

T

R
|
R

K

K

A

V

A

G

P

A

A

A

L

L

A

A

G

G

C

C

T

T

M

V

V

V

K
|
K

P

P

A

A

P

E

Q

D

T

T

P

P

F

F

T

S

A

A

L

L

A

A

L

L

A

A

V

E

L

L

A

A

E

S

R

Q

A

A

G

G

I

I

P

P

A

S

G

G

V

V

F

Y

S

N

V

V

I

I

-

P

-

C

-

S

T

R

G

K

D

N

A

A

I

K

A

E

I

V

G

G

N

E

E

A

M

I

C

C

T

T

N

D

P

P

I

L

V

V

R

S

K

K

L

I

S

S

F

F

T

T

G

G

S

S

T

T

N

T

V

T

G

G

I

K

K

I

L

L

M

L

A

H

Q

H

C

A

A

A

P

N

T

S

L

V

K

K

K

R

L

V

S

S

L

M

E
|
E

L

L

G

G

N

L

A

A

P

P

F

F

I

I

V

V

F

F

D

D

D

S

A

A

N

N

I

V

D

D

A

Q

A

A

V

V

E

A

G

G

A

A

M

M

I

A

A

S

K

K

Y

F

R
|
R

N

N

A

T

G

G

Q

Q

T

T

C
x
A

V

V

C

C

A

S

N

N

R

Q

I

F

Y

L

V

V

Q

Q

A

R

G

G

V

I

Y

H

D

D

E

A

F

F

A

V

E

K

K

A

L

F

S

A

M

E

A

A

V

M

A

K

K

K

-

N

L

L

K

R

V

V

G

G

E

N

G

G

I

F

I

E

A

E

G

G

V

T

T

T

T

Q

G

G

P

P

L

L

I

I

N

N

A

E

A

K

A

A

V

V

E

E

K

K

V

V

Q

E

S

K

H

Q

L

V

E

N

D

D

A

A

I

V

K

S

K

K

G

G

A

A

T

T

V

V

L

V

A

T

G

G

K

K

V

R

H

H

E

Q

L

L

G

G

G

K

N

N

F

F

E

E

P

P

T

T

V

L

L

L

T

C

N

N

A

V

D

T

K

Q

S

D

M

M

R

L

V

C

A

T

R

H

E

E

E
|
E

T

T

F

F

G

G

P

P

L

L

A

A

P

P

L

V

F

I

K

K

F

F

N

D

D

T

V

E

D

E

V

A

I

I

K

A

Q

I

A

A

N

N

D

A

T

A

E

D

F

V

G

G

L

L

A

A

A

G

Y

Y

F

F

Y

Y

G

S

R

Q

D

D

I

P

S

A

L

Q

V

I

W

W

K

R

V

V

A

A

E

E

S

Q

L

L

E

E

Y

V

G

G

M

M

V

V

G

G

V

V

N

N

T

E

G

G

L

L

I

I

S
|
S

T

S

E

V

V

E

A

C

P

P

F

F

G

G

M

V

K

K

S

Q

S

S

G

G

L

L

G

G

R

R

E
|
E

G

G

S

S

K

K

Y

Y

G

G

I

I

E

D

E

E

Y

Y

L

L

E

E

I

L

K

K

Y

Y

I

V

C

C

active site: N155 (= N157), K178 (= K180), E256 (= E255), A290 (≠ C289), E388 (= E386), E465 (= E463)
binding succinic acid: R163 (= R165), R284 (= R283), A290 (≠ C289), S448 (= S446)

5x5uA Crystal structure of alpha-ketoglutarate-semialdehyde dehydrogenase (kgsadh) complexed with NAD (see paper)
41% identity, 97% coverage: 12:480/482 of query aligns to 6:474/476 of 5x5uA

query
sites
5x5uA

Q

Q

C

L

Y

L

I

I

N

D

G

G

Q

E

W

W

C

V

D
|
D

A
|
A

N
x
A

S

S

K
x
G

E

K

T

T

V

I

A

D

I

V

T

V

N

N

P

P

A

A

T

T

G

G

A

K

V

P

I

I

A

G

C

R

V

V

P

A

V

H

M

A

G

G

Q

I

A

A

E

D

T

L

Q

D

A

R

A

A

I

L

A

A

E

Q

A

S

A

G

L

F

P

E

A

A

W

W

R

R

A

K

L

V

T

P

A

A

K

H

E

E

R

R

G

A

A

A

K

T

L

M

R

R

R

K

W

A

F

A

E

A

L

L

L

V

N

R

E

E

N

R

S

A

D

D

D

A

L

I

A

A

L

Q

L

L

M

M

T

T

S

Q

E

E

Q

Q

G

G

K

K

P

P

L

L

T

T

E

E

A

A

K

R

G

V

E

E

V

V

T

L

Y

S

A

A

S

D

F

I

I

I

E

E

W

W

F

F

A

A

E

D

E

E

A

G

K

R

R

R

I

V

Y

Y

G

G

D

R

T

I

I

V

P

P

G

P

H

R

Q

N

G

L

D

G

K

A

R

Q

I

Q

M

T

V

V

I

V

K

K

Q

E

P

P

V

V

G

G

V

P

T

V

A

A

I

F

T

T

P
|
P

W

W

N
|
N

F

F

P

P

A

V

A

N

M

Q

I

V

T

V

R

R

K

K

A

L

A

S

P

A

A

A

L

L

A

A

A

T

G

G

C

C

T

S

M

F

V

L

V

V

K
|
K

P

A

A

P

P

E

Q

E

T

T

P

P

F

A

T

S

A

P

L

A

A

A

L

L

A

L

V

R

L

A

A

F

E

V

R

D

A

A

G

G

I

V

P

P

A

A

G

G

V

V

F

I

S

G

V

L

I

V

T

Y

G

G

D

D

A
x
P

I
x
A

A

E

I

I

G
x
S

N

S

E

Y

M

L

C

I

T

P

N

H

P

P

I

V

V

I

R

R

K

K

L

V

S

T

F

F

T

T

G
|
G

S
|
S

T

T

N

P

V
|
V

G

G

I

K

K

Q

L

L

M

A

A

S

Q

L

C

A

A

G

P

L

T

H

L

M

K

K

K

R

L

A

S

T

L

M

E
|
E

L

L

G

G

N

H

A

A

P

P

F

V

I

I

V

V

F

A

D

E

D

D

A

A

N

D

I

V

D

A

A

L

A

A

V

V

E

K

G

A

A

A

M

G

I

G

A

A

K

K

Y

F

R

R

N

N

A

A

G

G

Q

Q

T

V

C
|
C

V

I

C

S

A

P

N

T

R

R

I

F

Y

L

V

V

Q

H

A

N

G

S

V

I

Y

R

D

D

E

E

F

F

A

T

E

R

K

A

L

L

S

V

M

K

A

H

V

A

A

E

K

G

L

L

K

K

V

V

G

G

E

N

G

G

I

L

I

E

A

E

G

G

V

T

T

T

T

L

G

G

P

A

L

L

I

A

N

N

A

P

A
x
R

V

L

E

T

K

A

V

M

Q

A

S

S

H

V

L

I

E

D

D

N

A

A

I

R

K

K

K

V

G

G

A

A

T

S

V

I

L

E

A

T

G

G

K

E

V

R

H

I

E

G

L

S

G

E

G

G

N

N

F

F

E

A

P

P

T

T

V

V

L

I

T

A

N

N

A

V

D

P

K

L

S

D

M

A

R

D

V

V

A

F

R

N

E

N

E
|
E

T

P

F
|
F

G

G

P

P

L

V

A

A

P

A

L

I

F

R

K

G

F

F

N

D

D

K

V

L

D

E

V

A

I

I

K

A

Q

E

A

A

N

N

D

R

T

L

E

P

F

F

G

G

L

L

A

A

A

G

Y

Y

F

A

Y

F

G

T

R

R

D

S

I

F

S

A

L

N

V

V

W

H

K

L

V

L

A

T

E

Q

S

R

L

L

E

E

Y

V

G

G

M

M

V

L

G

W

V

I

N

N

T

Q

G

P

L

A

I

T

S

P

T

W

E

P

V

E

A

M

P

P

F

F

G

G

M

V

K

K

S

D

S

S

G

G

L

Y

G

G

R

S

E
|
E

G

G

S

G

K

P

Y

E

G

A

I

L

E

E

E

P

Y

Y

L

L

E

V

I

T

K

K

Y

S

I

V

C

T

M

V

active site: N151 (= N157), K174 (= K180), E249 (= E255), C283 (= C289), E380 (= E386), E457 (= E463)
binding glycerol: D15 (= D21), A16 (= A22), A17 (≠ N23), G19 (≠ K25)
binding nicotinamide-adenine-dinucleotide: P149 (= P155), P207 (≠ A213), A208 (≠ I214), S211 (≠ G217), G227 (= G233), S228 (= S234), V231 (= V237), R329 (≠ A335), R330 (≠ A336), E380 (= E386), F382 (= F388)

5x5tA Crystal structure of alpha-ketoglutarate semialdehyde dehydrogenase (kgsadh) from azospirillum brasilense (see paper)
41% identity, 97% coverage: 12:480/482 of query aligns to 6:474/476 of 5x5tA

query
sites
5x5tA

Q

Q

C

L

Y

L

I

I

N

D

G

G

Q

E

W

W

C

V

D

D

A

A

N

A

S

S

K

G

E

K

T

T

V

I

A

D

I

V

T

V

N

N

P

P

A

A

T

T

G

G

A

K

V

P

I

I

A

G

C

R

V

V

P

A

V

H

M

A

G

G

Q

I

A

A

E

D

T

L

Q

D

A

R

A

A

I

L

A

A

E

Q

A

S

A

G

L

F

P

E

A

A

W

W

R

R

A

K

L

V

T

P

A

A

K

H

E

E

R

R

G

A

A

A

K

T

L

M

R

R

R

K

W

A

F

A

E

A

L

L

L

V

N

R

E

E

N

R

S

A

D

D

D

A

L

I

A

A

L

Q

L

L

M

M

T

T

S

Q

E

E

Q

Q

G

G

K

K

P

P

L

L

T

T

E

E

A

A

K

R

G

V

E

E

V

V

T

L

Y

S

A

A

S

D

F

I

I

I

E

E

W

W

F

F

A

A

E

D

E

E

A

G

K

R

R

R

I

V

Y

Y

G

G

D

R

T

I

I

V

P

P

G

P

H

R

Q

N

G

L

D

G

K

A

R

Q

I

Q

M

T

V

V

I

V

K

K

Q

E

P

P

V

V

G

G

V

P

T

V

A

A

I

F

T

T

P

P

W

W

N
|
N

F

F

P

P

A

V

A

N

M

Q

I

V

T

V

R

R

K

K

A

L

A

S

P

A

A

A

L

L

A

A

A

T

G

G

C

C

T

S

M

F

V

L

V

V

K
|
K

P

A

A

P

P

E

Q

E

T

T

P

P

F

A

T

S

A

P

L

A

A

A

L

L

A

L

V

R

L

A

A

F

E

V

R

D

A

A

G

G

I

V

P

P

A

A

G

G

V

V

F

I

S

G

V

L

I

V

T

Y

G

G

D

D

A

P

I

A

A

E

I

I

G

S

N

S

E

Y

M

L

C

I

T

P

N

H

P

P

I

V

V

I

R

R

K

K

L

V

S

T

F

F

T

T

G

G

S

S

T

T

N

P

V

V

G

G

I

K

K

Q

L

L

M
x
A

A

S

Q

L

C

A

A

G

P

L

T

H

L

M

K

K

K

R

L

A

S

T

L

M

E
|
E

L

L

G

G

N

H

A

A

P

P

F

V

I

I

V

V

F

A

D

E

D

D

A

A

N

D

I

V

D

A

A

L

A

A

V

V

E

K

G

A

A

A

M

G

I

G

A

A

K

K

Y

F

R

R

N

N

A

A

G

G

Q

Q

T

V

C
|
C

V

I

C

S

A

P

N

T

R

R

I

F

Y

L

V

V

Q

H

A

N

G

S

V

I

Y

R

D

D

E

E

F

F

A

T

E

R

K

A

L

L

S

V

M

K

A

H

V

A

A

E

K

G

L

L

K

K

V

V

G

G

E

N

G

G

I

L

I

E

A

E

G

G

V

T

T

T

T

L

G

G

P

A

L

L

I

A

N

N

A

P

A

R

V

L

E

T

K

A

V

M

Q

A

S

S

H

V

L

I

E

D

D

N

A

A

I

R

K

K

K

V

G

G

A

A

T

S

V

I

L

E

A

T

G

G

K

E

V

R

H

I

E

G

L

S

G

E

G

G

N

N

F

F

E

A

P

P

T

T

V

V

L

I

T

A

N

N

A

V

D

P

K

L

S

D

M

A

R

D

V

V

A

F

R

N

E

N

E
|
E

T

P

F

F

G

G

P

P

L

V

A

A

P

A

L

I

F

R

K

G

F

F

N

D

D

K

V

L

D

E

V

A

I

I

K

A

Q

E

A

A

N

N

D

R

T

L

E

P

F

F

G

G

L

L

A

A

A

G

Y

Y

F

A

Y

F

G

T

R

R

D

S

I

F

S

A

L

N

V

V

W

H

K

L

V

L

A

T

E

Q

S

R

L

L

E

E

Y

V

G

G

M

M

V

L

G

W

V

I

N

N

T

Q

G

P

L

A

I

T

S

P

T

W

E

P

V

E

A

M

P

P

F

F

G

G

M

V

K

K

S

D

S

S

G

G

L
x
Y

G

G

R

S

E
|
E

G

G

S

G

K

P

Y

E

G

A

I

L

E

E

E

P

Y

Y

L

L

E

V

I

T

K

K

Y

S

I

V

C

T

M

V

active site: N151 (= N157), K174 (= K180), E249 (= E255), C283 (= C289), E380 (= E386), E457 (= E463)
binding glycerol: A236 (≠ M242), Y454 (≠ L460)

6j76A Structure of 3,6-anhydro-l-galactose dehydrogenase in complex with nap (see paper)
39% identity, 96% coverage: 12:476/482 of query aligns to 3:471/477 of 6j76A

query
sites
6j76A

Q

Q

C

M

Y

Y

I

V

N

G

G

G

Q

E

W

W

C

I

D

D

A

A

N

S

S

N

K

G

E

Q

T

T

V

E

A

P

I

V

T

V

N

S

P

P

A

I

T

N

G

E

A

E

V

V

I

L

A

A

C

Y

V

I

P

Q

V

D

M

A

G

D

Q

A

A

S

E

D

T

A

Q

E

A

R

A

V

I

L

A

A

A

V

A

A

E

T

A

T

A

A

L

Q

P

K

A

E

W

W

R

A

A

K

L

Q

T

P

A

A

K

R

E

Q

R

R

G

A

A

E

K

V

L

L

R

R

R

K

W

F

F

A

E

Q

L

L

L

I

N

R

E

D

N

N

S

K

D

Q

D

Y

L

L

A

A

L

E

L

L

M

L

T

V

S

K

E

E

Q

Q

G

G

K

K

P

L

L

L

T

K

E

V

A

A

K

L

G

G

E

E

V

V

T

E

Y

A

A

T

A

S

S

T

F

F

I

I

E

E

W

Y

F

A

A

C

E

D

E

W

A

A

K

R

R

Q

I

M

Y

D

G

G

D

D

T

I

I

V

P

K

G

S

H

D

Q

N

G

A

D

N

K

E

R

Q

I

I

M

M

V

I

I

H

K

K

Q

I

P

P

V

R

G

G

V

V

T

V

A

V

A

A

I
|
I

T
|
T

P
x
A

W
|
W

N
|
N

F

F

P

P

A

L

A

A

M

L

I

A

T

G

R

R

K

K

A

I

A

G

P

P

A

A

L

L

A

V

A

A

G

G

C

N

T

S

M

I

V

V

K
|
K

P

P

A
x
T

P
x
S

Q

E

T

T

P

P

F

L

T

A

A

T

L

L

A

E

L

L

A

G

V

Y

L

L

A

A

E

E

R

Q

A

A

G

G

I

I

P

P

A

A

G

G

V

V

F

L

S

N

V

I

I

V

T

T

G

G

D

G

A
x
G

I

R

A

T

I

L

G
|
G

N

N

E

E

M

L

C

V

T

G

N

H

P

R

I

M

V

T

R

N

K

M

L

V

S

S

F

M

T
|
T

G
|
G

S
|
S

T

T

N

P

V
x
A

G

G

I

Q

K
x
S

L
x
I

M

I

A

R

Q

A

C

S

A

A

P

N

T

N

L

M

K

A

K

H

L

V

S

Q

L

L

E
|
E

L
|
L

G

G

N

K

A

A

P

P

F

F

I

I

V

V

F

M

D

E

D

D

A

A

N

D

I

L

D

E

A

Q

A

A

V

A

E

A

G

A

A

A

M

L

I

H

A

S

K

R

Y

F

R

D

N

N

A

C

G

G

Q

Q

T

V

C
|
C

V

T

C

C

A

N

N

E

R

R

I

M

Y

Y

V

V

Q

H

A

G

G

A

V

V

Y

Y

D

D

E

E

F

F

A

M

E

R

K

I

L

F

S

M

M

G

A

K

V

V

A

E

K

A

L

I

K

K

V

V

G

G

E

D

G

P

I

M

I

D

A

P

G

A

V

S

T

D

T

M

G

G

P

P

L

K

I

V

N

N

A

A

A

N

A

E

V

L

E

A

K

H

V

M

Q

E

S

E

H

L

L

V

E

A

D

E

A

A

I

V

K

D

K

E

G

G

A

A

T

T

V

V

L

L

A

F

G

G

K

K

V

K

H

L

E

E

-

G

-

P

-

E

-

F

L

E

G

K

G

G

N

F

F

W

F

F

E

E

P

P

T

T

V

V

L

L

T

T

N

N

A

V

D

T

K

Q

S

D

M

M

R

T

V

I

A

V

R

H

E

E

E
|
E

T

S

F
|
F

G

G

P

P

L

I

A

L

P

P

L

V

F

I

K

K

F

F

N

D

D

S

V

F

D

D

D

E

V

V

I

I

K

E

Q

Y

A

A

N

N

D

D

T

S

E

D

F

Y

G

G

L

L

A

A

Y

M

F

I

Y

C

G

T

R

Q

D

N

I

M

S

H

L

Y

V

I

W

N

K

R

V

L

A

L

E

T

S

E

L

L

E

E

Y

S

G

G

M

E

V

I

G

Y

V

V

N

N

T

R

G

G

L

H

I

G

S

E

T

Q

E

H

V

Q

A

G

P

F

F
x
H

G

N

G

G

M

Y

K

K

S

L

S

S

G

G

L

T

G

G

R

G

E
|
E

G

D

S

G

K

K

Y

Y

G

G

I

F

E

E

E

Q

Y

Y

L

L

E

E

I

K

K

K

active site: N148 (= N157), E246 (= E255), C280 (= C289), E458 (= E463)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I144 (= I153), T145 (= T154), A146 (≠ P155), W147 (= W156), N148 (= N157), K171 (= K180), T173 (≠ A182), S174 (≠ P183), G204 (≠ A213), G208 (= G217), T223 (= T232), G224 (= G233), S225 (= S234), A228 (≠ V237), S231 (≠ K240), I232 (≠ L241), E246 (= E255), L247 (= L256), C280 (= C289), E381 (= E386), F383 (= F388), H447 (≠ F452)

2impA Crystal structure of lactaldehyde dehydrogenase from e. Coli: the ternary complex with lactate (occupancy 0.5) and nadh. Crystals soaked with (l)-lactate. (see paper)
38% identity, 98% coverage: 9:481/482 of query aligns to 3:476/477 of 2impA

query
sites
2impA

L

V

R

Q

Q

H

Q

P

C

M

Y

Y

I

I

N

D

G

G

Q

Q

W

F

C

V

D

T

A

W

N

R

S

G

K

D

E

A

T

W

V

I

A

D

I

V

T

V

N

N

P

P

A

A

T

T

G

E

A

A

V

V

I

I

A

S

C

R

V

I

P

P

V

-

M

D

G

G

Q

Q

A

A

E

E

-

D

T

A

Q

R

A

K

A

A

I

I

A

D

A

A

E

E

A

R

A

A

L

Q

P

P

A

E

W

W

R

E

A

A

L

L

T

P

A

A

K

I

E

E

R

R

G

A

A

S

K

W

L

L

R

R

R

K

W

I

F

S

E

A

L

G

L

I

N

R

E

E

N

R

S

A

D

S

D

E

L

I

A

S

L

A

L

L

M

I

T

V

S

E

E

E

Q

G

G

G

K

K

P

I

L

Q

T

Q

E

L

A

A

K

E

G

V

E

E

V

V

T

A

Y

F

A

T

A

A

S

D

F

Y

I

I

E

D

W

Y

F

M

A

A

E

E

E

W

A

A

K

R

R

R

I

Y

Y

E

G

G

D

E

T

I

I

I

P

Q

G

S

H

D

Q

R

G

P

D

G

K

E

R

N

I

I

M

L

V

L

I

F

K

K

Q

R

P

A

V

L

G

G

V

V

T

T

A

T

A

G

I
|
I

T
x
L

P
|
P

W
|
W

N
|
N

F

F

P

P

A

F

M

L

I

I

T

A

R

R

K

K

A

M

A

A

P

P

A

A

L

L

A

L

A

T

G

G

C

N

T

T

M

I

V

V

V

I

K
|
K

P

P

A

S

P
x
E

Q
x
F

T

T

P

P

F

N

T

N

A

A

L

I

A

A

L

F

A

A

V

K

L

I

A

V

E

D

R

E

A

I

G

G

I

L

P

P

A

R

G

G

V

V

F

F

S

N

V

L

I

V

T

L

G

G

D

R

A
x
G

I

E

A

T

I

V

G
|
G

N
x
Q

E

E

M

L

C

A

T

G

N

N

P

P

I

K

V

V

R

A

K

M

L

V

S

S

F

M

T

T

G

G

S
|
S

T

V

N

S

V
x
A

G

G

I

E

K
|
K

L

I

M

M

A

A

Q

T

C

A

A

A

P

K

T

N

L

I

K

T

K

K

L

V

S

C

L

L

E
|
E

L

L

G

G

N

K

A

A

P

P

F

A

I

I

V

V

F

M

D

D

A

A

N

D

I

L

D

E

A

L

A

A

V

V

E

K

G

A

A

I

M

V

I

D

A

S

K

R

Y

V

R

I

N

N

A

S

G

G

Q

Q

T

V

C
|
C

V

N

C

C

A

A

N

E

R

R

I

V

Y

Y

V

V

Q

Q

A

K

G

G

V

I

Y

Y

D

D

E

Q

F

F

A

V

E

N

K

R

L

L

S

G

M

E

A

A

V

M

A

Q

K

A

L

V

K

Q

V

F

G

G

E

N

-

P

G

A

I

E

I

R

A

N

G

D

V

I

T

A

T

M

G

G

P

P

L

L

I

I

N

N

A

A

V

L

E

E

K
x
R

V

V

Q

E

S

Q

H

K

L

V

E

A

D

R

A

A

I

V

K

E

G

G

A

A

T

R

V

V

L

A

A

F

G

G

K

K

V

A

H

V

E

E

L

G

G

K

G

G

N

Y

F

Y

E

P

P

P

T

T

V

L

L

L

T

L

N

D

A

V

D

R

K

Q

S

E

M

M

R

S

V

I

A

M

R

H

E

E

E
|
E

T

T

F

F

G

G

P

P

L

V

A

L

P

P

L

V

F

V

K

A

F

F

N

D

D

T

V

L

D

E

D

D

V

A

I

I

K

S

Q

M

A

A

N

N

D

D

T

S

E

D

F

Y

G

G

L

L

A

T

A

S

Y

S

F

I

Y

Y

G

T

R

Q

D

N

I

L

S

N

L

V

V

A

W

M

K

K

V

A

A

I

E

K

S

G

L

L

E

K

Y

F

G

G

M

E

V

T

G

Y

V

I

N

N

T

R

G

E

L

N

I

F

S

E

T

A

E

M

V

Q

A

G

P

F

F

H

G

A

G

G

M

W

K

R

S

K

S

S

G

G

L

I

G

G

R

G

E
x
A

G

D

S

G

K

K

Y

H

G

G

I

L

E

H

E

E

Y

Y

L

L

E

Q

I

T

K

Q

Y

V

I

V

C

Y

M

L

S

Q

active site: N151 (= N157), K174 (= K180), E249 (= E255), C283 (= C289), E381 (= E386), A458 (≠ E463)
binding 1,4-dihydronicotinamide adenine dinucleotide: I147 (= I153), L148 (≠ T154), P149 (= P155), W150 (= W156), K174 (= K180), E177 (≠ P183), F178 (≠ Q184), G207 (≠ A213), G211 (= G217), Q212 (≠ N218), S228 (= S234), A231 (≠ V237), K234 (= K240), R334 (≠ K339)

2iluA Crystal structure of lactaldehyde dehydrogenase from e. Coli: the binary complex with NADPH (see paper)
38% identity, 98% coverage: 9:481/482 of query aligns to 3:476/477 of 2iluA

query
sites
2iluA

L

V

R

Q

Q

H

Q

P

C

M

Y

Y

I

I

N

D

G

G

Q

Q

W

F

C

V

D

T

A

W

N

R

S

G

K

D

E

A

T

W

V

I

A

D

I

V

T

V

N

N

P

P

A

A

T

T

G

E

A

A

V

V

I

I

A

S

C

R

V

I

P

P

V

-

M

D

G

G

Q

Q

A

A

E

E

-

D

T

A

Q

R

A

K

A

A

I

I

A

D

A

A

E

E

A

R

A

A

L

Q

P

P

A

E

W

W

R

E

A

A

L

L

T

P

A

A

K

I

E

E

R

R

G

A

A

S

K

W

L

L

R

R

R

K

W

I

F

S

E

A

L

G

L

I

N

R

E

E

N

R

S

A

D

S

D

E

L

I

A

S

L

A

L

L

M

I

T

V

S

E

E

E

Q

G

G

G

K

K

P

I

L

Q

T

Q

E

L

A

A

K

E

G

V

E

E

V

V

T

A

Y

F

A

T

A

A

S

D

F

Y

I

I

E

D

W

Y

F

M

A

A

E

E

E

W

A

A

K

R

R

R

I

Y

Y

E

G

G

D

E

T

I

I

I

P

Q

G

S

H

D

Q

R

G

P

D

G

K

E

R

N

I

I

M

L

V

L

I

F

K

K

Q

R

P

A

V

L

G

G

V

V

T

T

A

T

A

G

I
|
I

T
x
L

P
|
P

W
|
W

N
|
N

F

F

P

P

A

F

M

L

I

I

T

A

R

R

K

K

A

M

A

A

P

P

A

A

L

L

A

L

A

T

G

G

C

N

T

T

M

I

V

V

V

I

K
|
K

P

P

A
x
S

P
x
E

Q

F

T

T

P

P

F

N

T

N

A

A

L

I

A

A

L

F

A

A

V

K

L

I

A

V

E

D

R

E

A

I

G

G

I

L

P

P

A

R

G

G

V

V

F

F

S

N

V

L

I

V

T

L

G

G

D
x
R

A
x
G

I

E

A

T

I

V

G
|
G

N
x
Q

E

E

M

L

C

A

T

G

N

N

P

P

I

K

V

V

R

A

K

M

L

V

S

S

F

M

T

T

G

G

S
|
S

T

V

N

S

V
x
A

G

G

I

E

K
|
K

L
x
I

M

M

A

A

Q

T

C

A

A

A

P

K

T

N

L

I

K

T

K

K

L

V

S

C

L

L

E
|
E

L

L

G

G

N

K

A

A

P

P

F

A

I

I

V

V

F

M

D

D

A

A

N

D

I

L

D

E

A

L

A

A

V

V

E

K

G

A

A

I

M

V

I

D

A

S

K

R

Y

V

R

I

N

N

A

S

G

G

Q

Q

T

V

C
|
C

V

N

C

C

A

A

N

E

R

R

I

V

Y

Y

V

V

Q

Q

A

K

G

G

V

I

Y

Y

D

D

E

Q

F

F

A

V

E

N

K

R

L

L

S

G

M

E

A

A

V

M

A

Q

K

A

L

V

K

Q

V

F

G

G

E

N

-

P

G

A

I

E

I

R

A

N

G

D

V

I

T

A

T

M

G

G

P

P

L

L

I

I

N
|
N

A

A

V

L

E

E

K
x
R

V

V

Q

E

S

Q

H

K

L

V

E

A

D

R

A

A

I

V

K

E

G

G

A

A

T

R

V

V

L

A

A

F

G

G

K

K

V

A

H

V

E

E

L

G

G

K

G

G

N

Y

F

Y

E

P

P

P

T

T

V

L

L

L

T

L

N

D

A

V

D

R

K

Q

S

E

M

M

R

S

V

I

A

M

R

H

E

E

E
|
E

T

T

F
|
F

G

G

P

P

L

V

A

L

P

P

L

V

F

V

K

A

F

F

N

D

D

T

V

L

D

E

D

D

V

A

I

I

K

S

Q

M

A

A

N

N

D

D

T

S

E

D

F

Y

G

G

L

L

A

T

A

S

Y

S

F

I

Y

Y

G

T

R

Q

D

N

I

L

S

N

L

V

V

A

W

M

K

K

V

A

A

I

E

K

S

G

L

L

E

K

Y

F

G

G

M

E

V

T

G

Y

V

I

N

N

T

R

G

E

L

N

I

F

S

E

T

A

E

M

V

Q

A

G

P

F

F

H

G

A

G

G

M

W

K

R

S

K

S

S

G

G

L

I

G

G

R

G

E
x
A

G

D

S

G

K

K

Y

H

G

G

I

L

E

H

E

E

Y

Y

L

L

E

Q

I

T

K

Q

Y

V

I

V

C

Y

M

L

S

Q

active site: N151 (= N157), K174 (= K180), E249 (= E255), C283 (= C289), E381 (= E386), A458 (≠ E463)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I147 (= I153), L148 (≠ T154), P149 (= P155), W150 (= W156), K174 (= K180), S176 (≠ A182), E177 (≠ P183), R206 (≠ D212), G207 (≠ A213), G211 (= G217), Q212 (≠ N218), S228 (= S234), A231 (≠ V237), K234 (= K240), I235 (≠ L241), N328 (= N333), R334 (≠ K339), F383 (= F388)

P25553 Lactaldehyde dehydrogenase; Aldehyde dehydrogenase A; Glycolaldehyde dehydrogenase; EC 1.2.1.22; EC 1.2.1.21 from Escherichia coli (strain K12) (see 5 papers)
38% identity, 98% coverage: 9:481/482 of query aligns to 5:478/479 of P25553

query
sites
P25553

L

V

R

Q

Q

H

Q

P

C

M

Y

Y

I

I

N

D

G

G

Q

Q

W

F

C

V

D

T

A

W

N

R

S

G

K

D

E

A

T

W

V

I

A

D

I

V

T

V

N

N

P

P

A

A

T

T

G

E

A

A

V

V

I

I

A

S

C

R

V

I

P

P

V

-

M

D

G

G

Q

Q

A

A

E

E

-

D

T

A

Q

R

A

K

A

A

I

I

A

D

A

A

E

E

A

R

A

A

L

Q

P

P

A

E

W

W

R

E

A

A

L

L

T

P

A

A

K

I

E

E

R

R

G

A

A

S

K

W

L

L

R

R

R

K

W

I

F

S

E

A

L

G

L

I

N

R

E

E

N

R

S

A

D

S

D

E

L

I

A

S

L

A

L

L

M

I

T

V

S

E

E

E

Q

G

G

G

K

K

P

I

L

Q

T

Q

E

L

A

A

K

E

G

V

E

E

V

V

T

A

Y

F

A

T

A

A

S

D

F

Y

I

I

E

D

W

Y

F

M

A

A

E

E

E

W

A

A

K

R

R

R

I

Y

Y

E

G

G

D

E

T

I

I

I

P

Q

G

S

H

D

Q

R

G

P

D

G

K

E

R

N

I

I

M

L

V

L

I

F

K

K

Q

R

P

A

V

L

G

G

V

V

T

T

A

T

A

G

I

I

T
x
L

P

P

W

W

N

N

F

F

P

P

A

F

M

L

I

I

T

A

R
|
R

K

K

A

M

A

A

P

P

A

A

L

L

A

L

A

T

G

G

C

N

T

T

M

I

V

V

V

I

K
|
K

P
|
P

A
x
S

P
x
E

Q
x
F

T

T

P

P

F

N

T

N

A

A

L

I

A

A

L

F

A

A

V

K

L

I

A

V

E

D

R

E

A

I

G

G

I

L

P

P

A

R

G

G

V

V

F

F

S

N

V

L

I

V

T

L

G

G

D

R

A

G

I

E

A

T

I

V

G

G

N
x
Q

E

E

M

L

C

A

T

G

N

N

P

P

I

K

V

V

R

A

K

M

L

V

S

S

F

M

T

T

G

G

S
|
S

T

V

N

S

V

A

G

G

I

E

K

K

L

I

M

M

A

A

Q

T

C

A

A

A

P

K

T

N

L

I

K

T

K

K

L

V

S

C

L

L

E
|
E

L

L

G

G

N

K

A

A

P

P

F

A

I

I

V

V

F

M

D

D

A

A

N

D

I

L

D

E

A

L

A

A

V

V

E

K

G

A

A

I

M

V

I

D

A

S

K

R

Y

V

R

I

N

N

A

S

G

G

Q

Q

T

V

C

C

V
x
N

C

C

A

A

N

E

R

R

I

V

Y

Y

V

V

Q

Q

A

K

G

G

V

I

Y

Y

D

D

E

Q

F

F

A

V

E

N

K

R

L

L

S

G

M

E

A

A

V

M

A

Q

K

A

L

V

K

Q

V

F

G

G

E

N

-

P

G

A

I

E

I

R

A

N

G

D

V

I

T

A

T

M

G

G

P

P

L

L

I

I

N

N

A

A

V

L

E

E

K
x
R

V

V

Q

E

S

Q

H

K

L

V

E

A

D

R

A

A

I

V

K

E

G

G

A

A

T

R

V

V

L

A

A

F

G

G

K

K

V

A

H

V

E

E

L

G

G

K

G

G

N

Y

F

Y

E

P

P

P

T

T

V

L

L

L

T

L

N

D

A

V

D

R

K

Q

S

E

M

M

R

S

V

I

A

M

R

H

E

E

T

T

F

F

G

G

P

P

L

V

A

L

P

P

L

V

F

V

K

A

F

F

N

D

D

T

V

L

D

E

D

D

V

A

I

I

K

S

Q

M

A

A

N

N

D

D

T

S

E

D

F

Y

G

G

L

L

A

T

A

S

Y

S

F

I

Y

Y

G

T

R

Q

D

N

I

L

S

N

L

V

V

A

W

M

K

K

V

A

A

I

E

K

S

G

L

L

E

K

Y

F

G

G

M

E

V

T

G

Y

V

I

N

N

T

R

G

E

L

N

I

F

S
x
E

T

A

E

M

V

Q

A

G

P

F

F
x
H

G

A

G

G

M

W

K

R

S

K

S

S

G

G

L

I

G

G

R

G

E

A

G

D

S

G

K

K

Y

H

G

G

I

L

E

H

E

E

Y

Y

L

L

E

Q

I

T

K

Q

Y

V

I

V

C

Y

M

L

S

Q

L150 (≠ T154) binding NAD(+)
R161 (= R165) binding (S)-lactate
KPSE 176:179 (≠ KPAP 180:183) binding NAD(+)
F180 (≠ Q184) mutation to T: Can bind and use NADP(+) as coenzyme. 16-fold increase in catalytic efficiency with NAD(+) as coenzyme.
Q214 (≠ N218) binding NAD(+)
S230 (= S234) binding NAD(+)
E251 (= E255) binding (S)-lactate
N286 (≠ V290) binding (S)-lactate; mutation to E: 4-fold increase in catalytic efficiency with L-lactaldehyde as substrate. Shows expanded substrate specificity.; mutation to H: 15-fold increase in catalytic efficiency with L-lactaldehyde as substrate. Shows expanded substrate specificity.; mutation to T: 6-fold increase in catalytic efficiency with L-lactaldehyde as substrate. Shows expanded substrate specificity.
R336 (≠ K339) binding NAD(+)
E443 (≠ S446) binding (S)-lactate
H449 (≠ F452) binding (S)-lactate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2opxA Crystal structure of lactaldehyde dehydrogenase from escherichia coli
37% identity, 98% coverage: 9:481/482 of query aligns to 3:476/477 of 2opxA

query
sites
2opxA

L

V

R

Q

Q

H

Q

P

C

M

Y

Y

I

I

N

D

G

G

Q

Q

W

F

C

V

D

T

A

W

N

R

S

G

K

D

E

A

T

W

V

I

A

D

I

V

T

V

N

N

P

P

A

A

T

T

G

E

A

A

V

V

I

I

A

S

C

R

V

I

P

P

V

-

M

D

G

G

Q

Q

A

A

E

E

-

D

T

A

Q

R

A

K

A

A

I

I

A

D

A

A

E

E

A

R

A

A

L

Q

P

P

A

E

W

W

R

E

A

A

L

L

T

P

A

A

K

I

E

E

R

R

G

A

A

S

K

W

L

L

R

R

R

K

W

I

F

S

E

A

L

G

L

I

N

R

E

E

N

R

S

A

D

T

D

E

L

I

A

S

L

A

L

L

M

I

T

V

S

E

E

E

Q

G

G

G

K

K

P

I

L

Q

T

Q

E

L

A

A

K

E

G

V

E

E

V

V

T

A

Y
x
F

A

T

A

A

S

D

F

Y

I

I

E

D

W

Y

F

M

A

A

E

E

E

W

A

A

K

R

R

R

I

Y

Y

E

G

G

D

E

T

I

I

I

P

Q

G

S

H

D

Q

R

G

P

D

G

K

E

R

N

I

I

M

L

V

L

I

F

K

K

Q

R

P

A

V

L

G

G

V

V

T

T

A

T

A

G

I

I

T

L

P

P

W

W

N
|
N

F
|
F

P

P

A

F

M

L

I

I

T

A

R

R

K

K

A

M

A

A

P

P

A

A

L

L

A

L

A

T

G

G

C

N

T

T

M

I

V

V

V

I

K
|
K

P

P

A

S

P

E

Q

F

T

T

P

P

F

N

T

N

A

A

L

I

A

A

L

F

A

A

V

K

L

I

A

V

E

D

R

E

A

I

G

G

I

L

P

P

A

R

G

G

V

V

F

F

S

N

V

L

I

V

T

L

G

G

D

R

A

G

I

E

A

T

I

V

G

G

N

Q

E

E

M

L

C

A

T

G

N

N

P

P

I

K

V

V

R

A

K

M

L

V

S

S

F

M

T

T

G

G

S

S

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N

S

V

A

G

G

I

E

K

K

L

I

M

M

A

A

Q

T

C

A

A

A

P

K

T

N

L

I

K

T

K

K

L

V

S

C

L

L

E
|
E

L

L

G

G

N

K

A

A

P

P

F

A

I

I

V

V

F

M

D

D

A

A

N

D

I

L

D

E

A

L

A

A

V

V

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K

G

A

A

I

M

V

I

D

A

S

K

R

Y

V

R

I

N

N

A

S

G

G

Q

Q

T

V

C
|
C

V
x
N

C

C

A

A

N

E

R

R

I

V

Y

Y

V

V

Q

Q

A

K

G

G

V

I

Y

Y

D

D

E

Q

F

F

A

V

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N

K

R

L

L

S

G

M

E

A

A

V

M

A

Q

K

A

L

V

K

Q

V
x
F

G
|
G

E

N

-

P

G

A

I

E

I
x
R

A

N

G
x
D

V
x
I

T
x
A

T

M

G

G

P

P

L

L

I

I

N

N

A

A

V

L

E

E

K

R

V

V

Q

E

S

Q

H

K

L

V

E

A

D

R

A

A

I

V

K

E

G

G

A

A

T

R

V

V

L

A

A

L

G

G

K

K

V

A

H

V

E

E

L

G

G

K

G

G

N

Y

F
x
Y

F

Y

E

P

P

P

T

T

V

L

L

L

T

L

N

D

A

V

D

R

K

Q

S

E

M

M

R

S

V

I

A

M

R

H

E

E

E
|
E

T

T

F

F

G

G

P

P

L

V

A

L

P

P

L

V

F

V

K

A

F

F

N

D

D

T

V

L

D

E

V

A

I

I

K

S

Q

M

A

A

N

N

D

D

T

S

E

D

F

Y

G

G

L

L

A

T

A

S

Y

S

F

I

Y

Y

G

T

R

Q

D

N

I

L

S

N

L

V

V

A

W

M

K

K

V

A

A

I

E

K

S

G

L

L

E

K

Y

F

G

G

M

E

V

T

G

Y

V

I

N

N

T

R

G

E

L

N

I
x
F

S
x
E

T

A

E

M

V

Q

A

G

P

F

F

H

G

A

G

G

M

W

K

R

S

K

S

S

G

G

L

I

G

G

R

G

E
x
A

G

D

S

G

K

K

Y

H

G

G

I

L

E

H

E

E

Y

Y

L

L

E

Q

I

T

K

Q

Y

V

I

V

C

Y

M

L

S

Q

active site: N151 (= N157), K174 (= K180), E249 (= E255), C283 (= C289), E381 (= E386), A458 (≠ E463)
binding (3alpha,5beta,12alpha)-3,12-dihydroxycholan-24-oic acid: F105 (≠ Y111), F152 (= F158), N284 (≠ V290), F312 (≠ V318), G313 (= G319), R318 (≠ I323), D320 (≠ G325), I321 (≠ V326), A322 (≠ T327), Y362 (≠ F367), F440 (≠ I445), F440 (≠ I445), E441 (≠ S446)

4pz2B Structure of zm aldh2-6 (rf2f) in complex with NAD (see paper)
40% identity, 99% coverage: 1:478/482 of query aligns to 1:484/494 of 4pz2B

query
sites
4pz2B

M

L

L

L

L

F

N

D

D

V

P

P

S

E

L

I

L

R

R

F

Q

T

Q

K

C

L

Y

F

I

I

N

N

G

G

Q

S

W

F

C

V

D

D

A

A

N

V

S

S

K

G

E

R

T

T

V

F

A

D

I

T

T

R

N

D

P

P

A

R

T

T

G

G

A

G

V

V

I

I

A

A

C

S

V

V

P

A

V

E

M

A

G

D

Q

K

A

E

E

D

T

V

Q

D

A

L

A

A

I

V

A

R

A

A

E

R

A

A

L

F

P

D

A

H

-

G

-

E

W

W

R

P

A

R

L

M

T

S

A

G

K

S

E

E

R

R

G

G

A

R

K

I

L

M

R

A

R

R

W

L

F

A

E

D

L

L

L

V

N

E

E

E

N

H

S

A

D

G

D

E

L

L

A

A

L

A

L

L

M

E

T

S

S

L

E

D

Q

A

G

G

K

K

-

H

P

P

L

A

T

V

E

T

A

R

K

A

G

V

E

D

V

V

T

G

Y

N

A

A

S

G

F

S

I

L

E

R

W

Y

F

F

A

A

E

G

E

A

A

A

K

D

R

K

I

I

Y

H

G

G

D

E

T

T

I

L

-

K

-

M

P

P

G

G

H

Q

-

F

Q

Q

G

G

D

H

K

-

R

-

I

-

M

-

V

T

I

L

K

R

Q

E

P

P

V

L

G

G

V

V

T

A

A

G

A

V

I
|
I

T
x
I

P
|
P

W
|
W

N
|
N

F

F

P

P

A

S

A

T

M
|
M

I

F

T

A

R

V

K

K

A

V

A

A

P

P

A

A

L

L

A

A

G

G

C

C

T

A

M

L

V

V

K
|
K

P

P

A
|
A

P
x
E

Q

Q

T

T

P

P

F

L

T

S

A

A

L

L

A

Y

L

L

A

A

V

Q

L

L

A

A

E

K

R

Q

A

A

G

G

I

V

P

P

A

D

G

G

V

V

F

I

S

N

V

V

I

V

T

P

G

G

D

F

A
x
G

I

P

A

T

I

A

G
|
G

N

A

E

A

M

L

C

A

T

S

N

H

P

M

I

D

V

V

R

D

K

M

L

V

S

S

F
|
F

T
|
T

G
|
G

S
|
S

T

T

N

E

V
|
V

G

G

I

R

K

L

L

I

M

M

A

K

Q

A

C

S

A

A

P

E

T

S

-

N

L

L

K

K

K

P

L

V

S

Y

L

L

E
|
E

L
|
L

G

G

N

K

A

S

P

P

F

L

I

I

V

V

F

F

D

D

A

A

N

D

I

L

D

D

A

M

A

A

V

V

E

E

G

L

A

A

M

V

I

G

A

A

K

S

Y

F

R

F

N

N

A

K

G

G

Q

E

T

A

C
|
C

V

V

C

A

A

A

N

S

R

R

I

V

Y

Y

V

V

Q

Q

A

E

G

R

V

V

Y

Y

D

D

E

R

F

F

A

E

E

E

K

R

L

L

S

A

M

E

A

R

V

M

A

R

K

S

L

W

K

V

V

V

G

G

E

D

G

P

I

F

I

S

-

D

A

P

G

R

V

A

T

D

T

Q

G

G

P

P

L

Q

I

V

N

D

A

K

A

A

A

Q

V

Y

E

E

K

R

V

V

Q

L

S

S

H

Y

L

I

E

D

D

H

A

G

I

K

K

R

K

E

G

G

A

A

T

T

V

L

L

L

A

T

G

G

K

R

-

P

-

C

V

A

H

P

E

E

L

G

G

K

G

G

N

Y

F

Y

F

I

E

E

P

P

T

T

V

V

L

F

T

T

N

N

A

V

D

K

K

E

S

D

M

M

R

I

V

I

A

A

R

K

E

E

E
|
E

T

I

F
|
F

G

G

P

P

L

V

A

M

P

C

L

L

F

M

K

K

F

F

N

K

D

T

V

V

D

E

V

A

I

I

K

A

Q

R

A

A

N

N

D

D

T

T

E

R

F

Y

G

G

L

L

A

G

A

A

Y

G

F

V

Y

V

G

T

R

R

D

D

I

L

S

D

L

V

V

A

W

N

K

R

V

V

A

V

E

R

S

S

L

V

E

R

Y

A

G

G

M

V

V

V

G

W

V

V

N

N

T

C

G

Y

L

F

I

A

S

M

T

G

E

S

V

D

A

C

P

P

F

F

G

G

M

R

K

K

S

M

S

S

G

G

L

F

G

G

R

K

E
x
D

G

E

S

G

K

M

Y

H

G

A

I

L

E

D

E

K

Y

Y

L

L

E

A

I

V

K

K

Y

S

I

V

active site: N159 (= N157), K182 (= K180), E258 (= E255), C292 (= C289), E392 (= E386), D469 (≠ E463)
binding nicotinamide-adenine-dinucleotide: I155 (= I153), I156 (≠ T154), P157 (= P155), W158 (= W156), N159 (= N157), M164 (= M162), K182 (= K180), A184 (= A182), E185 (≠ P183), G215 (≠ A213), G219 (= G217), F233 (= F231), T234 (= T232), G235 (= G233), S236 (= S234), V239 (= V237), E258 (= E255), L259 (= L256), C292 (= C289), E392 (= E386), F394 (= F388)

Q3SY69 Mitochondrial 10-formyltetrahydrofolate dehydrogenase; Mitochondrial 10-FTHFDH; mtFDH; Aldehyde dehydrogenase family 1 member L2; EC 1.5.1.6 from Homo sapiens (Human) (see 2 papers)
39% identity, 97% coverage: 12:480/482 of query aligns to 444:921/923 of Q3SY69

query
sites
Q3SY69

Q

Q

C

C

Y

F

I

I

N

N

G

G

Q

Q

W

F

C

T

D

D

A

A

N

D

S

D

K

G

E

K

T

T

V

Y

A

D

I

T

T

I

N

N

P

P

A

T

T

D

G

G

A

S

V

T

I

I

A

C

C

K

V

V

P

S

V

Y

M

A

G

S

Q

L

A

A

E

D

T

V

Q

D

A

K

A

A

I

V

A

A

E

K

A

D

A

A

L

F

P

E

-

N

-

G

A

E

W

W

R

G

A

R

L

M

T

N

A

A

K

R

E

E

R

R

G

G

A

R

K

L

L

M

R

Y

R

R

W

L

F

A

E

D

L

L

N

E

E

E

N

N

S

Q

D

E

L

L

A

A

L

T

L

I

M

E

T

A

S

L

E

D

Q

S

G

G

K

A

P

V

L

Y

T

T

E

L

A

A

-

L

K

K

G

T

E

H

V

I

T

G

Y

M

A

S

A

V

S

Q

F

T

I

F

E

R

W

Y

F

F

A

A

E

G

E

W

A

C

K

D

R

K

I

I

Y

Q

G

G

D

S

T

T

I

I

P

P

G

I

H

N

Q

Q

G

A

D

R

K

P

R

N

-

R

-

N

-

L

I

T

M

F

V

T

I

K

K

K

Q

E

P

P

V

L

G

G

V

V

T

C

A

A

A

I

I

I

T

I

P

P

W

W

N

N

F

Y

P

P

A

L

A

M

M

M

I

L

T

A

R

W

K

K

A

S

A

A

P

A

A

C

L

L

A

A

G

G

C

N

T

T

M

L

V

V

V

L

K

K

P

P

A

A

P

Q

Q

V

T

T

P

P

F

L

T

T

A

A

L

L

A

K

L

F

A

A

V

E

L

L

A

S

E

V

R

K

A

A

G

G

I

F

P

P

A

K

G

G

V

V

F

I

S

N

V

I

I

I

T

P

G

G

D

S

A

G

I

G

A

I

I

A

G

G

N

Q

E

R

M

L

C

S

T

E

N

H

P

P

I

D

V

I

R

R

K

K

L

L

S

G

F

F

T

T

G

G

S

S

T

T

N

P

V

I

G

G

I

K

K

Q

L

I

M

M

A

K

Q

S

C

C

A

A

-

V

P

S

T

N

L

L

K

K

L

V

S

S

L

L

E

E

L

L

G

G

N

K

A

S

P

P

F

L

I

I

V

I

F

F

D

N

D

D

A

C

N

E

I

L

D

D

A

K

A

A

V

V

E

R

G

M

A

G

M

M

I

G

A

A

K

V

Y

F

R

F

N

N

A

K

G

G

Q

E

T

N

C

C

V

I

C

A

A

A

N

G

R

R

I

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Y

F

V

V

Q

E

A

E

G

S

V

I

Y

H

D

D

E

E

F

F

A

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T

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S

V

M

E

A

E

V

I

A

K

K

K

L

M

K

K

V

I

G

G

E

D

G

P

I

L

I

D

A

R

G

S

V

T

T

D

T

H

G

G

P

P

L

Q

I

N

N

H

A

K

A

A

A

H

V

L

E

E

K

K

V

L

Q

L

S

Q

H

Y

L

C

E

E

D

T

A

G

I

V

K

K

K

E

G

G

A

A

T

T

V

L

L

V

A

Y

G

G

K

R

V

Q

H

V

E

Q

L

R

G

P

G

G

N

F

F

F

F

M

E

E

P

P

T

T

V

V

L

F

T

T

N

D

A

V

D

E

K

D

S

Y

M

M

R

Y

V

L

A

A

R

K

E

E

T

S

F

F

G

G

P

P

L

I

A

M

P

V

L

I

F

S

K

K

F

F

N

Q

-

N

-

G

D

D

V

I

D

D

D

G

V

V

I

L

K

Q

Q

R

A

A

N

N

D

S

T

T

E

E

F

Y

G

G

L

L

A

A

A

S

Y

G

F

V

Y

F

G

T

R

R

D

D

I

I

S

N

L

K

V

A

W

M

K

Y

V

V

A

S

E

E

S

K

L

L

E

E

Y

A

G

G

M

T

V

V

G

F

V

I

N

N

T

T

G

Y

L

N

I

K

S

T

T

D

E

V

V

A

A

A

P

P

F

F

G

G

M

V

K

K

S

Q

S

S

G

G

L

F

G

G

R

K

E

D

G

L

S

G

K

E

Y

E

G

A

I

L

E

N

E

E

Y

Y

L

L

E

K

I

T

K

K

Y

T

I

V

C

T

M

L

Sites not aligning to the query:

133 P → H: found in a patient with neurodevelopmental disorder; uncertain significance; loss of formyltetrahydrofolate dehydrogenase activity
374 S→A: No effect on phosphopantetheinylation.
375 modified: O-(pantetheine 4'-phosphoryl)serine; S→A: Loss of phosphopantetheinylation.

5izdA Wild-type glyceraldehyde dehydrogenase from thermoplasma acidophilum in complex with NADP
37% identity, 98% coverage: 12:481/482 of query aligns to 4:476/494 of 5izdA

query
sites
5izdA

Q

K

C

L

Y

Y

I

I

N

D

G

G

Q

Q

W

W

C

V

D

N

A

S

N

S

S

S

K

G

E

K

T

T

V

V

A

D

I

K

T

Y

N

S

P

P

A

V

T

T

G

G

A

Q

V

V

I

I

A

G

C

R

V

F

P

E

V

A

M

A

G

T

Q

R

A

D

E

D

T

V

Q

D

A

R

A

A

I

I

A

D

A

A

E

E

A

D

A

A

L

F

P

W

A

A

W

W

R

N

A

D

L

L

T

G

A

S

K

V

E

E

R

R

G

S

A

K

K

I

L

I

R

Y

R

R

W

A

F

K

E

E

L

L

L

I

N

E

E

K

N

N

S

R

D

A

D

E

L

L

A

E

L

N

L

I

M

I

T

M

S

E

E

E

Q

N

G

G

K

K

P

P

L

V

T

K

E

E

A

A

K

K

G

E

E

E

V

V

T

D

Y

G

A

V

A

I

S

D

F

Q

I

I

E

Q

W

Y

F

Y

A

A

E

E

E

W

A

A

K

R

R

K

I

L

Y

N

G

G

D

E

T

V

I

V

P

E

G

G

H

T

Q

S

G

S

D

H

K

R

R

K

I

I

M

F

V

Q

I

Y

K

K

Q

V

P

P

V

Y

G

G

V

I

T

V

A

V

A

A

I
x
L

T
|
T

P

P

W
|
W

N
|
N

F

F

P

P

A

A

A

G

M

M

I

V

T

A

R

R

K

K

A

L

A

A

P

P

A

A

L

L

A

L

A

T

G

G

C

N

T

T

M

V

V

V

V

L

K
|
K

P
|
P

A
x
S

P
x
S

Q

D

T

T

P

P

F

G

T

S

A

A

L

E

A

W

L

I

A

V

V

R

L

K

A

F

E

V

R

E

A

A

G

G

I

V

P

P

A

K

G

G

V

V

F

L

S

N

V

F

I

I

T

T

G

G

D
x
R

A
x
G

I

S

A

E

I

I

G
|
G

N
x
D

E

Y

M

I

C

V

T

E

N

H

P

K

I

K

V

V

R

N

K

L

L

I

S

T

F

M

T

T

G
|
G

S
|
S

T

T

N

A

V
x
T

G

G

I

Q

K

R

L

I

M

M

A

Q

Q

K

C

A

A

S

P

A

T

N

L

M

K

A

K

K

L

L

S

I

L

L

E
|
E

L

L

G

G

N

K

A

A

P

P

F

F

I

M

V

V

F

W

D

K

D

D

A

A

N

D

I

M

D

D

A

N

A

A

V

L

E

K

G

T

A

L

M

L

I

W

A

A

K

K

Y

Y

R

W

N

N

A

A

G

G

Q

Q

T

S

C
|
C

V

I

C

A

A

A

N

E

R

R

I

L

Y

Y

V

V

Q

H

A

E

G

D

V

I

Y

Y

D

D

E

T

F

F

A

M

E

S

K

R

L

F

S

V

M

E

A

L

V

S

A

R

K

K

L

L

K

A

V

L

G

G

E

D

G

P

I

K

I

N

A

A

G

D

V

M

T

-

G

G

P

P

L

L

I

I

N

N

A

K

A

G

A

A

V

L

E

Q

K

A

V

T

Q

S

S

E

H

I

L

V

E

E

D

E

A

A

I

K

K

E

K

S

G

G

A

A

T

K

V

I

L

L

A

F

G

G

K

S

V

Q

H

P

E

S

L

L

-

S

-

G

-

P

-

Y

-

R

G

N

G

G

N

Y

F

F

E

L

P

P

T

T

V

I

L

I

T

G

N

N

A

A

D

D

K

Q

S

K

M

S

R

K

V

I

A

F

R

Q

E

E

E
|
E

T

I

F

F

G

A

P

P

L

V

A

I

P

G

L

A

F

R

K

K

F

I

N

S

D

S

V

V

D

E

V

M

I

Y

K

D

Q

L

A

A

N

N

D

D

T

S

E

K

F

Y

G

G

L

L

A

A

A

S

Y

Y

F

L

Y

F

G

T

R

K

D

D

I

P

S

N

L

I

V

I

W

F

K

E

V

A

A

S

E

E

S

R

L

I

E

R

Y

F

G

G

M

E

V

L

G

Y

V

V

N

N

T

M

G

P

L

G

I

P

S

E

T

A

E

S

V

Q

A

G

P

Y

F

H

G

T

G

G

M

F

K

R

S

M

S

T

G

G

L

Q

G

A

R

G

E
|
E

G

G

S

S

K

K

Y

Y

G

G

I

I

E

S

E

E

Y

Y

L

L

E

K

I

L

K

K

Y

N

I

I

C

Y

M

V

S

D

active site: N149 (= N157), K172 (= K180), E247 (= E255), C281 (= C289), E381 (= E386), E458 (= E463)
binding nadp nicotinamide-adenine-dinucleotide phosphate: L145 (≠ I153), T146 (= T154), W148 (= W156), K172 (= K180), P173 (= P181), S174 (≠ A182), S175 (≠ P183), R204 (≠ D212), G205 (≠ A213), G209 (= G217), D210 (≠ N218), G225 (= G233), S226 (= S234), T229 (≠ V237)

4pxlA Structure of zm aldh2-3 (rf2c) in complex with NAD (see paper)
39% identity, 97% coverage: 12:479/482 of query aligns to 7:477/486 of 4pxlA

query
sites
4pxlA

Q

K

C

L

Y

F

I

I

N

D

G

G

Q

K

W

F

C

V

D

D

A

A

N

V

S

S

K

G

E

K

T

T

V

F

A

E

I

T

T

R

N

D

P

P

A

R

T

T

G

G

A

E

V

V

I

I

A

A

C

S

V

I

P

A

V

E

M

G

G

D

Q

K

A

A

E

D

T

V

Q

D

A

L

A

A

I

V

A

K

A

A

E

R

A

E

A

A

L

F

P

D

-

N

-

G

A

P

W

W

R

P

A

R

L

M

T

T

A

G

K

Y

E

E

R

R

G

G

A

R

K

I

L

L

R

H

R

R

W

F

F

A

E

D

L

L

L

I

N

D

E

E

N

H

S

V

D

E

L

L

A

A

L

A

L

L

M

D

T

T

S

V

E

D

Q

A

G

G

K

K

P

L

L

F

T

A

E

V

A

G

K

K

G

A

-

R

E

D

V

I

T

P

Y

G

A

A

S

H

F

L

I

L

E

R

W

Y

F

Y

A

A

E

G

E

A

A

A

K

D

R

K

I

V

Y

H

G

G

D

A

T

T

I

L

P

K

G

M

H

A

Q

Q

G

-

D

R

K

M

R

H

I

G

M

Y

V

T

I

L

K

K

Q

E

P

P

V

V

G

G

V

V

T

V

A

G

A

H

I
|
I

T
x
V

P
|
P

W
|
W

N
|
N

F

Y

P

P

A

T

M

M

I

F

T

F

R

F

K

K

A

V

A

G

P

P

A

A

L

L

A

A

G

G

C

C

T

A

M

V

V

V

K
|
K

P

P

A

A

P
x
E

Q

Q

T

T

P

P

F

L

T

S

A

A

L

L

A

F

L

Y

A

A

V

H

L

L

A

A

E

R

R

E

A

A

G

G

I

V

P

P

A

A

G

G

V

V

F

L

S

N

V

V

I

V

T

P

G

G

D

F

A
x
G

I

P

A

T

I

A

G
|
G

N
x
A

E

A

M

V

C

A

T

A

N

H

P

M

I

D

V

V

R

D

K

K

L

V

S

S

F
|
F

T

T

G
|
G

S
|
S

T

T

N

E

V
|
V

G

G

I

R

K

L

L

V

M

M

A

R

Q

A

C

A

A

A

P

E

T

S

-

N

L

L

K

K

K

P

L

V

S

S

L

L

E
|
E

L

L

G
|
G

G

G

N

K

A

S

P

P

F

V

I

I

V

V

F

F

D

D

A

A

N

D

I

L

D

D

A

M

A

A

V

V

E

N

G

L

A

V

M

N

I

F

A

A

K

T

Y

Y

R

T

N

N

A

K

G

G

Q

E

T

I

C
|
C

V

V

C

A

A

G

N

T

R

R

I

I

Y

Y

V

V

Q

Q

A

E

G

G

V

I

Y

Y

D

D

E

E

F

F

A

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E

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A

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E

A

L

V

A

A

S

K

K

L

S

K

V

V

V

G

G

E

D

G

P

I

F

I

N

A

P

G

S

V

V

T

S

T

Q

G

G

P

P

L

Q

I

V

N

D

A

K

A

D

A
x
Q

V

Y

E

E

K
|
K

V

V

Q

L

S

R

H

Y

L

I

E

D

D

I

A

G

I

K

K

R

K

E

G

G

A

A

T

T

V

L

L

V

A

T

G

G

K

K

V

P

H

C

E

G

L

D

G

K

G

G

N

Y

F

Y

F

I

E

E

P

P

T

T

V

I

L

F

T

T

N

D

A

V

D

K

K

D

S

D

M

M

R

T

V

I

A

A

R

Q

E

D

E
|
E

T

I

F
|
F

G

G

P

P

L

V

A

M

P

A

L

L

F

M

K

K

F

F

N

K

D

T

V

V

D

E

V

V

I

I

K

Q

Q

K

A

A

N

N

D

N

T

T

E

R

F

Y

G

G

L

L

A

A

Y

G

F

I

Y

V

G

T

R

K

D

N

I

I

S

D

L

V

V

A

W

N

K

T

V

V

A

S

E

R

S

S

L

I

E

R

Y

A

G

G

M

A

V

I

G

W

V

I

N

N

T

C

G

Y

L

F

I

A

S

F

T

D

E

P

V

D

A

A

P

P

F

F

G

G

M

Y

K

K

S

M

S

S

G

G

L

F

G

G

R

K

E
x
D

G

M

S

G

K

M

Y

D

G

A

I

L

E

D

E

K

Y

Y

L

L

E

Q

I

T

K

K

Y

T

I

V

C

V

active site: N154 (= N157), K177 (= K180), E253 (= E255), C287 (= C289), E384 (= E386), D461 (≠ E463)
binding nicotinamide-adenine-dinucleotide: I150 (= I153), V151 (≠ T154), P152 (= P155), W153 (= W156), K177 (= K180), E180 (≠ P183), G210 (≠ A213), G214 (= G217), A215 (≠ N218), F228 (= F231), G230 (= G233), S231 (= S234), V234 (= V237), E253 (= E255), G255 (= G257), C287 (= C289), Q334 (≠ A336), K337 (= K339), E384 (= E386), F386 (= F388)

Q56YU0 Aldehyde dehydrogenase family 2 member C4; ALDH1a; Protein REDUCED EPIDERMAL FLUORESCENCE 1; EC 1.2.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
37% identity, 99% coverage: 6:480/482 of query aligns to 15:493/501 of Q56YU0

query
sites
Q56YU0

P

P

S

E

L

I

L

K

R

F

Q

T

Q

K

C

L

Y

F

I

I

N

N

G

G

Q

Q

W

F

C

I

D

D

A

A

N

A

S

S

K

G

E

K

T

T

V

F

A

E

I

T

T

I

N

D

P

P

A

R

T

N

G

G

A

E

V

V

I

I

A

A

C

T

V

I

P

A

V

E

M

G

G

D

Q

K

A

E

E

D

T

V

Q

D

A

L

A

A

I

V

A

N

A

A

E

R

A

Y

A

A

L

F

P

D

A

H

-

G

-

P

W

W

R

P

A

R

L

M

T

T

A

G

K

F

E

E

R

R

G

A

A

K

K

L

L

I

R

N

R

K

W

F

F

A

E

D

L

L

L

I

N

E

E

E

N

N

S

I

D

E

L

L

A

A

L

K

L

L

M

D

T

A

S

V

E

D

Q

G

G

G

K

K

P

L

L

F

T

Q

E

L

A

G

K

K

-

Y

G

A

E

D

V

I

T

P

Y

A

A

T

A

A

S

G

F

H

I

F

E

R

W

Y

F

N

A

A

E

G

E

A

A

A

K

D

R

K

I

I

Y

H

G

G

D

E

T

T

I

L

P

K

G

M

H

T

Q

R

G

Q

D

S

K

L

R

F

I
x
G

M

Y

V

T

I

L

K

K

Q

E

P

P

V

I

G

G

V

V

T

V

A

G

A

N

I

I

T

I

P

P

W

W

N

N

F

F

P

P

A

S

A

I

M

M

I

F

T

A

R

T

K

K

A

V

A

A

P

P

A

A

L

M

A

A

G

G

C

C

T

T

M

M

V

V

K

K

P

P

A

A

P

E

Q

Q

T

T

P

S

F

L

T

S

A

A

L

L

A

F

L

Y

A

A

V

H

L

L

A

S

E

K

R

E

A

A

G

G

I

I

P

P

A

D

G

G

V

V

F

L

S

N

V

I

I

V

T

T

G

G

D

F

A

G

I

S

A

T

I

A

G

G

N

A

E

A

M

I

C

A

T

S

N

H

P

M

I

D

V

V

R

D

K

K

L

V

S

S

F

F

T

T

G

G

S

S

T

T

N

D

V

V

G

G

I

R

K

K

L

I

M

M

-

Q

A

A

Q

A

C

A

A

A

P

S

T

N

L

L

K

K

L

V

S

S

L

L

E

E

L

L

G

G

N

K

A

S

P

P

F

L

I

L

V

I

F

F

D

N

D

D

A

A

N

D

I

I

D

D

A

K

A

A

V

A

E

D

G

L

A

A

M

L

I

L

A

G

K

C

Y

F

R

Y

N

N

A

K

G

G

Q

E

T

I

C

C

V

V

C

A

A

S

N

S

R

R

I

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Y

F

V

V

Q

Q

A

E

G

G

V

I

Y

Y

D

D

E

K

F

V

A

V

E

E

K

K

L

L

S

V

M

E

A

K

V

A

A

K

K

D

L

W

K

T

V

V

G

G

E

D

G

P

I

F

I

D

A

S

G

T

V

A

T

R

T

Q

G

G

P

P

L

Q

I

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N

D

A

K

A

R

A

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F

E

E

K

K

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I

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Y

L

I

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E

D

H

A

G

I

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N

K

E

G

G

A

A

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V

L

L

L

A

T

G

G

K

K

V

A

H

I

E

G

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D

G

K

G

G

N

Y

F

F

F

I

E

Q

P

P

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T

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I

L

F

T

A

N

D

A

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T

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E

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I

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M

K

K

F

F

N

K

D

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V

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E

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x
G

I

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K

Q

C

A

A

N

N

D

N

T

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E

K

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Y

G

G

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L

A

A

Y

G

F

I

Y

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Q

D

D

I

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D

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N

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T

V

V

A

S

E

R

S

S

L

I

E

K

Y

A

G

G

M

I

V

I

G

W

V

V

N

N

T

C

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Y

L

F

I

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F

T

D

E

L

V

D

A

C

P

P

F

Y

G

G

M

Y

K

K

S

M

S

S

G

G

L

N

G

C

R

R

E

E

G

S

S

G

K

M

Y

D

G

A

I

L

E

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N

Y

Y

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Y

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C

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M

M

G152 (≠ I140) mutation to E: In ref1-7; reduced activity on sinapaldehyde.
G416 (≠ V403) mutation to R: In ref1-6; reduced activity on sinapaldehyde.

7rluA Structure of aldh1l1 (10-formyltetrahydrofolate dehydrogenase) in complex with NADP (see paper)
37% identity, 97% coverage: 12:478/482 of query aligns to 104:579/583 of 7rluA

query
sites
7rluA

Q

Q

C

L

Y

F

I

I

N

G

G

G

Q

E

W

F

C

V

D

D

A

A

N

E

S

G

K

S

E

K

T

T

V

Y

A

N

I

T

T

I

N

N

P

P

A

T

T

D

G

G

A

S

V

V

I

I

A

C

C

Q

V

V

P

S

V

L

M

A

G

Q

Q

V

A

S

E

D

T

V

Q

D

A

K

A

A

I

V

A

A

E

K

A

E

A

A

L

F

P

E

-

N

-

G

A

L

W

W

R

G

A

K

L

I

T

N

A

A

K

R

E

D

R

R

G

G

A

R

K

L

L

L

R

Y

R

R

W

L

F

A

E

D

L

V

L

M

N

E

E

Q

N

H

S

Q

D

E

L

L

A

A

L

T

L

I

M

E

T

A

S

L

E

D

Q

A

G

G

K

A

P

V

L

Y

T

T

E

L

A

A

-

L

K
|
K

G

T

E

H

V

V

T

G

Y

M

A

S

A

I

S

Q

F

T

I

F

E

R

W

Y

F

F

A

A

E

G

E

W

A

C

K

D

R

K

I

I

Y

Q

G

G

D

A

T

T

I

I

P

P

G

I

H

N

Q

Q

G

A

D

R

K

P

R

N

-

R

-

N

-

L

I

T

M

L

V

T

I

K

K

K

Q

E

P

P

V

V

G

G

V

V

T

C

A

G

A

I

I

V

T

I

P

P

W

W

N

N

F

Y

P

P

A

L

A

M

M

M

I

L

T

S

R

W

K

K

A

T

A

A

P

A

A

C

L

L

A

A

G

G

C

N

T

T

M

V

V

V

V

I

K
|
K

P

P

A

A

P

Q

Q

V

T

T

P

P

F

L

T

T

A

A

L

L

A

K

L

F

A

A

V

E

L

L

A

T

E

L

R

K

A

A

G

G

I

I

P

P

A

K

G

G

V

V

F

V

S

N

V

I

I

L

T

P

G

G

D
x
S

A
x
G

I

S

A

L

I

V

G
|
G

N

Q

E

R

M

L

C

S

T

D

N

H

P

P

I

D

V

V

R

R

K

K

L

I

S

G

F

F

T

T

G
|
G

S
|
S

T

T

N

E

V
|
V

G

G

I

K

K

H

L

I

M

M

A

K

Q

S

C

C

A

A

-

L

P

S

T

N

L

V

K

K

L

V

S

S

L

L

E

E

L

L

G

G

N

K

A

S

P

P

F

L

I

I

V

I

F

F

D

A

D

D

A

C

N

D

I

L

D

N

A

K

A

A

V

V

E

Q

G

M

A

G

M

M

I

S

A

S

K

V

Y

F

R
x
F

N

N

A

K

G

G

Q

E

T
x
N

C
|
C

V

I

C

A

A

A

N

G

R

R

I

L

Y

F

V

V

Q

E

A

E

G

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V

I

Y

H

D

N

E

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F

F

A

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E

Q

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K

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V

M

E

A

E

V

V

A

E

K

K

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M

K

K

V

I

G

G

E

N

G

P

I

L

I

E

A

R

G

D

V

T

T

N

T

H

G

G

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P

L

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I

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N

H

A

E

A

A

A

H

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E

R

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E

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C

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G

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V

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H

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R

G

P

G

G

N

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F

F

E

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T

V

V

L

F

T

T

N

D

A

V

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E

K

D

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H

M

M

R

Y

V

I

A

A

R

K

E

E

T

S

F

F

G

G

P

P

L

I

A

M

P

I

L

I

F

S

K

R

F

F

-

A

-

D

N

G

D

D

V

V

D

D

D

A

V

V

I

L

K

S

Q

R

A

A

N

N

D

A

T

T

E

E

F

F

G

G

L

L

A

A

A

S

Y

G

F

V

Y

F

G

T

R

R

D

D

I

I

S

N

L

K

V

A

W

L

K

Y

V

V

A

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E

D

S

K

L

L

E

Q

Y

A

G

G

M

T

V

V

G

F

V

I

N

N

T

T

G

Y

L
x
N

I

K

S

T

T

D

E

V

V

A

A

A

P

P

F

F

G

G

M

F

K

K

S

Q

S

S

G

G

L

F

G

G

R

K

E

D

G

L

S

G

K

E

Y

A

G

A

I

L

E

N

E

E

Y

Y

L

L

E

R

I

I

K

K

Y

T

I

V

binding nadp nicotinamide-adenine-dinucleotide phosphate: K278 (= K180), S310 (≠ D212), G311 (≠ A213), G315 (= G217), G331 (= G233), S332 (= S234), V335 (= V237)
binding 4'-phosphopantetheine: K201 (= K106), F382 (≠ R283), N387 (≠ T288), C388 (= C289), N545 (≠ L444)

Query Sequence

>200453 FitnessBrowser__MR1:200453
MLLNDPSLLRQQCYINGQWCDANSKETVAITNPATGAVIACVPVMGQAETQAAIAAAEAA
LPAWRALTAKERGAKLRRWFELLNENSDDLALLMTSEQGKPLTEAKGEVTYAASFIEWFA
EEAKRIYGDTIPGHQGDKRIMVIKQPVGVTAAITPWNFPAAMITRKAAPALAAGCTMVVK
PAPQTPFTALALAVLAERAGIPAGVFSVITGDAIAIGNEMCTNPIVRKLSFTGSTNVGIK
LMAQCAPTLKKLSLELGGNAPFIVFDDANIDAAVEGAMIAKYRNAGQTCVCANRIYVQAG
VYDEFAEKLSMAVAKLKVGEGIIAGVTTGPLINAAAVEKVQSHLEDAIKKGATVLAGGKV
HELGGNFFEPTVLTNADKSMRVAREETFGPLAPLFKFNDVDDVIKQANDTEFGLAAYFYG
RDISLVWKVAESLEYGMVGVNTGLISTEVAPFGGMKSSGLGREGSKYGIEEYLEIKYICM
SV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory