SitesBLAST
Comparing 200843 FitnessBrowser__MR1:200843 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4zz7A Crystal structure of methylmalonate-semialdehyde dehydrogenase (dddc) from oceanimonas doudoroffii (see paper)
47% identity, 96% coverage: 3:481/499 of query aligns to 2:481/489 of 4zz7A
- active site: N149 (= N151), K172 (= K174), L246 (≠ F248), C280 (= C282), E382 (= E382), A462 (= A462)
- binding nicotinamide-adenine-dinucleotide: T146 (= T148), P147 (= P149), F148 (= F150), N149 (= N151), K172 (= K174), E175 (= E177), K205 (= K207), V208 (= V210), F222 (= F224), V223 (= V225), G224 (= G226), S225 (= S227), I228 (≠ V230), L246 (≠ F248), G247 (≠ A249), C280 (= C282), E382 (= E382), F384 (= F384)
5tjrD X-ray crystal structure of a methylmalonate semialdehyde dehydrogenase from pseudomonas sp. Aac (see paper)
49% identity, 96% coverage: 3:481/499 of query aligns to 1:455/468 of 5tjrD
- active site: N144 (= N151), K167 (= K174), L241 (≠ F248), C270 (= C282), E356 (= E382), A436 (= A462)
- binding adenosine-5'-diphosphate: I140 (= I147), T141 (= T148), F143 (= F150), K167 (= K174), E170 (= E177), K200 (= K207), F217 (= F224), S220 (= S227), I223 (≠ V230)
4iymC Crystal structure of putative methylmalonate-semialdehyde dehydrogenase from sinorhizobium meliloti 1021 complexed with NAD, target 011934
48% identity, 96% coverage: 1:481/499 of query aligns to 3:485/491 of 4iymC
- active site: N153 (= N151), K176 (= K174), F250 (= F248), C284 (= C282), E386 (= E382), Q466 (≠ A462)
- binding nicotinamide-adenine-dinucleotide: I149 (= I147), T150 (= T148), P151 (= P149), F152 (= F150), N153 (= N151), F154 (= F152), K176 (= K174), K209 (= K207), V212 (= V210), F226 (= F224), V227 (= V225), G228 (= G226), S229 (= S227), I232 (≠ V230), G251 (≠ A249), C284 (= C282), E386 (= E382), F388 (= F384)
1t90A Crystal structure of methylmalonate semialdehyde dehydrogenase from bacillus subtilis
44% identity, 96% coverage: 4:481/499 of query aligns to 4:479/484 of 1t90A
- active site: N151 (= N151), K174 (= K174), L248 (≠ F248), C282 (= C282), E380 (= E382), A460 (= A462)
- binding nicotinamide-adenine-dinucleotide: I147 (= I147), A148 (≠ T148), P149 (= P149), F150 (= F150), N151 (= N151), W159 (= W159), K174 (= K174), E177 (= E177), R178 (≠ Q178), H207 (≠ K207), V225 (= V225), G226 (= G226), S227 (= S227), V230 (= V230), L248 (≠ F248), T249 (≠ A249), C282 (= C282), E380 (= E382), F382 (= F384)
P42412 Malonate-semialdehyde dehydrogenase; MSA dehydrogenase; Methylmalonate-semialdehyde dehydrogenase; MMSA dehydrogenase; MMSDH; MSDH; EC 1.2.1.27 from Bacillus subtilis (strain 168) (see 3 papers)
44% identity, 96% coverage: 4:481/499 of query aligns to 6:481/487 of P42412
- C36 (≠ A34) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-160; A-287; A-351 and A-413.
- R107 (= R105) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- A150 (≠ T148) binding
- F152 (= F150) binding
- C160 (≠ L158) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-287; A-351 and A-413.
- K176 (= K174) binding
- E179 (= E177) binding
- R180 (≠ Q178) binding
- S229 (= S227) binding
- T251 (≠ A249) binding
- R283 (= R281) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- C287 (≠ I285) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-351 and A-413.
- C351 (≠ L348) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-413.
- E382 (= E382) binding
- C413 (≠ S413) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-351.
8vr1A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (ctp bound)
33% identity, 94% coverage: 8:477/499 of query aligns to 8:477/488 of 8vr1A
8vr0A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (gmp bound)
33% identity, 94% coverage: 8:477/499 of query aligns to 8:477/488 of 8vr0A
8vqzA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (cmp bound)
33% identity, 94% coverage: 8:477/499 of query aligns to 8:477/488 of 8vqzA
8vqwC Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (coa bound)
33% identity, 94% coverage: 8:477/499 of query aligns to 8:477/488 of 8vqwC
- binding coenzyme a: I147 (= I147), W150 (≠ F150), K174 (= K174), S176 (= S176), E177 (= E177), G207 (≠ K207), G211 (≠ D211), F225 (= F224), G227 (= G226), G228 (≠ S227), S231 (≠ V230), H331 (≠ A328), F387 (= F384)
8vj3A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (fad bound)
33% identity, 94% coverage: 8:477/499 of query aligns to 8:477/488 of 8vj3A
8uzoA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (adp bound)
33% identity, 94% coverage: 8:477/499 of query aligns to 8:477/488 of 8uzoA
8uznA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (amp bound)
33% identity, 94% coverage: 8:477/499 of query aligns to 8:477/488 of 8uznA
- binding adenosine monophosphate: I147 (= I147), G148 (≠ T148), K174 (= K174), S176 (= S176), E177 (= E177), G207 (≠ K207), G211 (≠ D211), F225 (= F224), G228 (≠ S227), S231 (≠ V230), K234 (≠ Y233)
8uzmA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (NADPH bound)
33% identity, 94% coverage: 8:477/499 of query aligns to 8:477/488 of 8uzmA
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G148 (≠ T148), W150 (≠ F150), K174 (= K174), S176 (= S176), E177 (= E177), G207 (≠ K207), G211 (≠ D211), F225 (= F224), T226 (≠ V225), G227 (= G226), G228 (≠ S227), S231 (≠ V230), E250 (≠ F248), G252 (= G250), C284 (= C282), E385 (= E382), F387 (= F384)
8uzkA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (NADP+ bound)
33% identity, 94% coverage: 8:477/499 of query aligns to 8:477/488 of 8uzkA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I147 (= I147), G148 (≠ T148), W150 (≠ F150), N151 (= N151), K174 (= K174), S176 (= S176), E177 (= E177), G207 (≠ K207), G211 (≠ D211), F225 (= F224), G227 (= G226), G228 (≠ S227), S231 (≠ V230), E250 (≠ F248), F387 (= F384)
8skfA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (lattice translocation disorder)
33% identity, 94% coverage: 8:477/499 of query aligns to 17:486/497 of 8skfA
- binding calcium ion: T33 (≠ V24), I34 (≠ T25), D100 (≠ E91), V187 (≠ Q178)
- binding nicotinamide-adenine-dinucleotide: I156 (= I147), G157 (≠ T148), A158 (≠ P149), W159 (≠ F150), K183 (= K174), E186 (= E177), G216 (≠ K207), G220 (≠ D211), T235 (≠ V225), G236 (= G226), G237 (≠ S227), S240 (≠ V230), K243 (≠ Y233), E259 (≠ F248), C293 (= C282), F396 (= F384)
6wsbA Crystal structure of a betaine aldehyde dehydrogenase from burkholderia pseudomallei bound to cofactor NAD (see paper)
32% identity, 95% coverage: 6:477/499 of query aligns to 7:477/489 of 6wsbA
- active site: N152 (= N151), E250 (≠ F248), C284 (= C282), E462 (≠ Q460)
- binding nicotinamide-adenine-dinucleotide: I148 (= I147), G149 (≠ T148), A150 (≠ P149), W151 (≠ F150), N152 (= N151), K175 (= K174), E178 (= E177), G208 (≠ K207), G211 (≠ V210), A212 (≠ D211), F225 (= F224), T226 (≠ V225), G227 (= G226), G228 (≠ S227), T231 (≠ V230), V235 (≠ I234), E250 (≠ F248), L251 (≠ A249), G252 (= G250), C284 (= C282), E385 (= E382), F387 (= F384)
5gtlA NADPH complex structure of aldehyde dehydrogenase from bacillus cereus
32% identity, 96% coverage: 1:477/499 of query aligns to 13:486/491 of 5gtlA
- active site: N165 (= N151), K188 (= K174), E263 (≠ F248), C297 (= C282), E394 (= E382), E471 (≠ Q460)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I161 (= I147), P163 (= P149), K188 (= K174), A190 (≠ S176), E191 (= E177), Q192 (= Q178), G221 (≠ D206), G225 (≠ V210), G241 (= G226), S242 (= S227), T245 (≠ V230), L264 (≠ A249), C297 (= C282), E394 (= E382), F396 (= F384)
5gtkA NAD+ complex structure of aldehyde dehydrogenase from bacillus cereus
32% identity, 96% coverage: 1:477/499 of query aligns to 13:486/491 of 5gtkA
- active site: N165 (= N151), K188 (= K174), E263 (≠ F248), C297 (= C282), E394 (= E382), E471 (≠ Q460)
- binding nicotinamide-adenine-dinucleotide: I161 (= I147), I162 (≠ T148), P163 (= P149), W164 (≠ F150), K188 (= K174), E191 (= E177), G221 (≠ D206), G225 (≠ V210), A226 (≠ D211), F239 (= F224), G241 (= G226), S242 (= S227), T245 (≠ V230), Y248 (= Y233), L264 (≠ A249), C297 (= C282), Q344 (≠ A328), R347 (= R331), E394 (= E382), F396 (= F384)
4cazA Crystal structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa in complex with nadh
32% identity, 95% coverage: 6:477/499 of query aligns to 7:478/489 of 4cazA
- active site: N152 (= N151), K175 (= K174), E251 (≠ F248), C285 (= C282), E386 (= E382), E463 (≠ Q460)
- binding [[(2R,3S,4R,5R)-5-[(3R)-3-aminocarbonyl-3,4-dihydro-2H-pyridin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanidyl-phosphoryl] [(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl phosphate: I148 (= I147), G149 (≠ T148), W151 (≠ F150), N152 (= N151), K175 (= K174), E178 (= E177), G208 (≠ K207), G212 (≠ D211), F226 (= F224), T227 (≠ V225), G228 (= G226), G229 (≠ S227), T232 (≠ V230), V236 (≠ I234), E251 (≠ F248), L252 (≠ A249), C285 (= C282), E386 (= E382), F388 (= F384)
2woxA Betaine aldehyde dehydrogenase from pseudomonas aeruginosa with NAD(p) h-catalytic thiol adduct. (see paper)
32% identity, 95% coverage: 6:477/499 of query aligns to 7:478/489 of 2woxA
- active site: N152 (= N151), K175 (= K174), E251 (≠ F248), C285 (= C282), E386 (= E382), E463 (≠ Q460)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I148 (= I147), G149 (≠ T148), W151 (≠ F150), N152 (= N151), K175 (= K174), S177 (= S176), E178 (= E177), G208 (≠ K207), G212 (≠ D211), F226 (= F224), T227 (≠ V225), G228 (= G226), G229 (≠ S227), T232 (≠ V230), V236 (≠ I234), E251 (≠ F248), L252 (≠ A249), C285 (= C282), E386 (= E382), F388 (= F384)
Query Sequence
>200843 FitnessBrowser__MR1:200843
MTTQVKHYIDGEFTAGTGTSQIVVTNPANNATIAVINSATADEVHAAIASAKAAFKTWKE
VPVSERARVMLRYQHLLKEHHDELATILAHETGKTFEDAKGDVWRGIEVAEHACNIASLL
MGETVENVARSIDTYSYTQPLGVCAGITPFNFPAMIPLWMFPLAIACGNTFILKPSEQDP
MTPQRLVELFVEAGAPKGVLQLIHGDKTAVDILLADPAVKAISFVGSVAVGQYIYKTGTD
NLKRVQAFAGAKNHCVIMPDANKQQVINNLVGASVGAAGQRCMAISVAVFVGAAKEWIPE
LKEALAKVRPGLWDDKDAGYGPLISPAAKVRVLKLIAQGKEEGAQCLLDGSDFTVAGFES
GNWVGPTMFTKVTTDMSIYKEEIFGPVLCCMESDSLEDAIELVNASPYGNGTSIFTASGA
AARKYQHEIEVGQVGINVPIPVPLPFFSFTGWKGSFYGDQHAYGKQAVRFYTETKTITAR
WFESDIAVAAGPNMSINLR
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory